TP53

UniProt ID: P04637
Organism: Homo sapiens
Review Status: COMPLETE
Aliases:
p53 tumor protein p53 cellular tumor antigen p53 phosphoprotein p53
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Gene Description

Tumor suppressor p53 is a multifunctional transcription factor that acts as a guardian of the genome, coordinating cellular responses to diverse stress signals including DNA damage, oxidative stress, hypoxia, and metabolic stress. It regulates cell fate decisions through transcriptional control of genes involved in cell cycle arrest, apoptosis, senescence, DNA repair, and metabolism. TP53 is the most frequently mutated gene in human cancers (~50%), underlining its critical role in preventing malignant transformation. Beyond its canonical tumor suppressive functions, p53 also regulates ferroptosis, autophagy, immune responses, and stemness.

Existing Annotations Review

GO Term Evidence Action Reason
GO:0000976 transcription cis-regulatory region binding
IEA
GO_REF:0000002 		ACCEPT
Summary: TP53 is a well-established DNA-binding transcription factor that directly binds to p53 response elements in target gene promoters. This is one of its core molecular functions, supported by extensive experimental evidence including the IDA annotations from PMID:15710329 and PMID:17996705.
Reason: This annotation accurately represents a core molecular function of p53. Multiple lines of experimental evidence confirm p53 directly binds cis-regulatory regions to regulate transcription.
Supporting Evidence:
PMID:15710329
Human MUC1 oncoprotein regulates p53-responsive gene transcription
PMID:17996705
An acetylation switch in p53 mediates holo-TFIID recruitment
file:human/TP53/TP53-deep-research-falcon.md
model: Edison Scientific Literature
GO:0000122 negative regulation of transcription by RNA polymerase II
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 functions as a transcriptional repressor for genes including BCL2 and cell cycle genes.
Reason: Core function - transcriptional repression is part of p53's regulatory activity.
Supporting Evidence:
TP53-deep-research
Repression: Downregulates anti-apoptotic genes (BCL2), cell cycle progression genes
PMID:10329733
p53 suppresses the activation of the Bcl-2 promoter by the Brn-3a POU family transcription factor.
PMID:19749791
Sep 14. Repression of SHP-1 expression by p53 leads to trkA tyrosine phosphorylation and suppression of breast cancer cell proliferation.
GO:0000423 mitophagy
IEA
GO_REF:0000107 		ACCEPT
Summary: TP53 has complex roles in autophagy and mitophagy regulation, with both promoting and inhibiting functions depending on context. There is also a negative regulation of mitophagy annotation (GO:1901525), suggesting context-dependent regulation.
Reason: p53 regulates mitophagy through multiple mechanisms including transcriptional control of mitophagy-related genes. This is part of its broader role in cellular stress response and metabolism.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
GO; GO:0006914; P:autophagy; IMP:CAFA
TP53-deep-research
Accumulating evidence has shown that p53 also regulates cell metabolism, ferroptosis, tumor microenvironment, autophagy and so on
GO:0001701 in utero embryonic development
IEA
GO_REF:0000107 		KEEP AS NON CORE
Summary: TP53 plays roles in embryonic development, though p53 knockout mice are viable, indicating it is not absolutely essential. Development-related functions are context-specific rather than core functions.
Reason: While p53 has developmental roles, these are not its primary functions. p53-null mice can complete embryonic development, though with increased cancer susceptibility.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
GO; GO:0001701; P:in utero embryonic development; IEA:Ensembl ... GO; GO:0048568; P:embryonic organ development; IEA:Ensembl
TP53-deep-research
Although p53 plays roles in development and embryogenesis, these are often secondary to its core tumor suppressor functions and may represent over-annotation in certain contexts
GO:0001756 somitogenesis
IEA
GO_REF:0000107 		KEEP AS NON CORE
Summary: Somitogenesis is a specific developmental process. While p53 may influence developmental processes, this is a highly specific annotation that is not a core function.
Reason: Very specific developmental process that is not central to p53 function. p53 knockout mice complete somitogenesis successfully.
Supporting Evidence:
TP53-deep-research
Although p53 plays roles in development and embryogenesis, these are often secondary to its core tumor suppressor functions
GO:0001836 release of cytochrome c from mitochondria
IEA
GO_REF:0000107 		ACCEPT
Summary: TP53 promotes apoptosis through the mitochondrial pathway, including regulation of cytochrome c release via transcriptional targets like BAX and PUMA, as well as direct mitochondrial functions.
Reason: Core component of p53-mediated intrinsic apoptotic pathway. p53 induces pro-apoptotic BCL2 family members that trigger cytochrome c release.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression
TP53-deep-research
Transcriptional: Activation of pro-apoptotic genes (PUMA, BAX, NOXA, FAS); Non-transcriptional: Direct mitochondrial translocation and interaction with BCL-2 family proteins
GO:0002309 T cell proliferation involved in immune response
IEA
GO_REF:0000107 		KEEP AS NON CORE
Summary: TP53 has emerging roles in immune regulation, but specific T cell proliferation is not a core function. This may relate to non-cell-autonomous tumor suppression through immune modulation.
Reason: While p53 influences immune responses, direct regulation of T cell proliferation is not a primary function. This is a context-specific, non-core role.
GO:0002326 B cell lineage commitment
IEA
GO_REF:0000107 		KEEP AS NON CORE
Summary: B cell lineage commitment is a specific hematopoietic developmental process. While p53 may influence hematopoiesis, this is not a core function.
Reason: Highly specific developmental process in the immune system. Not a primary function of p53 as a tumor suppressor.
GO:0002360 T cell lineage commitment
IEA
GO_REF:0000107 		KEEP AS NON CORE
Summary: T cell lineage commitment is a specific developmental process in hematopoiesis. Not a core p53 function.
Reason: Specific immune system developmental process that is not central to p53 tumor suppressor function.
GO:0002931 response to ischemia
IEA
GO_REF:0000107 		ACCEPT
Summary: TP53 responds to hypoxic stress and ischemia as part of its cellular stress response functions. This involves HIF pathway interactions and metabolic regulation.
Reason: Part of p53s broader cellular stress response function. Ischemia/hypoxia is a well-documented p53-activating stress signal.
Supporting Evidence:
GO:1990144
intrinsic apoptotic signaling pathway in response to hypoxia
GO:0006302 double-strand break repair
IEA
GO_REF:0000107 		ACCEPT
Summary: TP53 coordinates DNA damage response including regulation of DNA repair genes. It promotes both repair and apoptosis depending on damage severity.
Reason: Core function of p53 as guardian of the genome. p53 regulates multiple DNA repair pathways through transcriptional control of repair genes.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
induces cell cycle arrest, DNA repair or apoptosis
GO:0006357 regulation of transcription by RNA polymerase II
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 regulates RNA polymerase II transcription as both activator and repressor.
Reason: Core molecular function - p53 is a sequence-specific RNA pol II transcription factor.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:30089260
p53 Regulates the Expression of LRP1 and Apoptosis through a Stress Intensity-Dependent MicroRNA Feedback Loop.
GO:0006606 protein import into nucleus
IEA
GO_REF:0000107 		REMOVE
Summary: This likely refers to p53s own nuclear import rather than it regulating import of other proteins. p53 nuclear localization is critical for its function but this annotation is unclear.
Reason: Ambiguous annotation - p53 undergoes nuclear import but does not regulate protein import into nucleus as a general function. This appears to be a misannotation.
Supporting Evidence:
TP53-deep-research
Nuclear Localization Signals (NLS): Location: Residues 305-322 and 369-375; Function: Nuclear import and localization [refers to p53s own import, not regulation of other proteins]
GO:0006974 DNA damage response
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 is central to the DNA damage response, being activated by and coordinating responses to DNA damage.
Reason: Core function - p53 is the 'guardian of the genome' that coordinates DNA damage responses.
Supporting Evidence:
TP53-deep-research
p53 serves as a central node in the DNA damage response network
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:30089260
p53 Regulates the Expression of LRP1 and Apoptosis through a Stress Intensity-Dependent MicroRNA Feedback Loop.
PMID:24356969
2013 Dec 19. Rbm24, an RNA-binding protein and a target of p53, regulates p21 expression via mRNA stability.
PMID:17938203
Oct 15. Protein kinase C delta induces transcription of the TP53 tumor suppressor gene by controlling death-promoting factor Btf in the apoptotic response to DNA damage.
PMID:15710329
Human MUC1 oncoprotein regulates p53-responsive gene transcription in the genotoxic stress response.
GO:0006979 response to oxidative stress
IEA
GO_REF:0000120 		ACCEPT
Summary: TP53 is activated by oxidative stress and regulates antioxidant responses, ROS metabolism, and cell fate decisions under oxidative conditions.
Reason: Core stress response function. p53 responds to oxidative stress and regulates redox balance through multiple target genes.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type
GO:0007179 transforming growth factor beta receptor signaling pathway
IEA
GO_REF:0000107 		KEEP AS NON CORE
Summary: TP53 has complex interactions with TGF-beta signaling, but this is not a core function. There is also annotation for negative regulation of this pathway (GO:0030512).
Reason: While p53 interacts with TGF-beta signaling, this is a context-specific crosstalk rather than a core p53 function.
GO:0007346 regulation of mitotic cell cycle
IEA
GO_REF:0000107 		ACCEPT
Summary: Cell cycle regulation is a fundamental function of TP53. It induces cell cycle arrest through p21/CDKN1A and other cell cycle regulators.
Reason: Core tumor suppressor function. p53 induces cell cycle checkpoints in response to stress, preventing damaged cells from proliferating.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Negatively regulates cell division by controlling expression of a set of genes required for this process
GO:0007369 gastrulation
IEA
GO_REF:0000107 		KEEP AS NON CORE
Summary: Gastrulation is a specific early developmental process. p53 is not essential for gastrulation as p53-null mice complete this process.
Reason: Highly specific developmental process that is not a core p53 function. p53 knockout mice undergo normal gastrulation.
GO:0007405 neuroblast proliferation
IEA
GO_REF:0000107 		KEEP AS NON CORE
Summary: Neuroblast proliferation is a specific neural developmental process. Not a core p53 function.
Reason: Specific developmental process in nervous system. p53 may regulate proliferation broadly but neuroblast-specific regulation is not core.
GO:0007406 negative regulation of neuroblast proliferation
IEA
GO_REF:0000107 		KEEP AS NON CORE
Summary: p53 negatively regulates proliferation broadly, but neuroblast-specific regulation is a specialized context.
Reason: While p53 inhibits proliferation generally, neuroblast-specific regulation is not a core function but rather a tissue-specific manifestation.
GO:0007417 central nervous system development
IEA
GO_REF:0000107 		KEEP AS NON CORE
Summary: CNS development is a broad developmental process. p53 has roles but is not essential as p53-null mice have functional CNS.
Reason: Developmental process that is not core to p53 tumor suppressor function. p53-null mice develop functional nervous systems.
GO:0007507 heart development
IEA
GO_REF:0000107 		KEEP AS NON CORE
Summary: Heart development is a specific organ developmental process. Not essential as p53-null mice have normal hearts.
Reason: Organ-specific developmental process that is not a core p53 function. p53 knockout mice develop normal hearts.
GO:0008156 negative regulation of DNA replication
IEA
GO_REF:0000107 		ACCEPT
Summary: TP53 inhibits DNA replication as part of cell cycle arrest, preventing replication of damaged DNA.
Reason: Core function related to cell cycle control and preventing propagation of damaged DNA. Part of p53s tumor suppressor activity.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Negatively regulates cell division
GO:0008283 cell population proliferation
IEA
GO_REF:0000107 		REMOVE
Summary: This is a very general term. p53 primarily NEGATIVELY regulates proliferation. The annotation GO:0008285 (negative regulation) is more accurate.
Reason: Too general and potentially misleading. p53 primarily inhibits rather than promotes proliferation. The negative regulation annotation is more appropriate.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Negatively regulates cell division by controlling expression of a set of genes required for this process
GO:0009303 rRNA transcription
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 can regulate ribosomal biogenesis and rRNA transcription as part of metabolic control, typically inhibiting it under stress.
Reason: p53 regulates ribosome biogenesis and protein synthesis capacity, typically suppressing rRNA transcription under stress conditions.
GO:0009410 response to xenobiotic stimulus
IEA
GO_REF:0000120 		ACCEPT
Summary: TP53 responds to various xenobiotic stresses including drugs and toxins that cause DNA damage or cellular stress.
Reason: Part of p53s broad stress response function. Many xenobiotics activate p53 through DNA damage or other stress pathways.
GO:0009411 response to UV
IEA
GO_REF:0000120 		ACCEPT
Summary: UV radiation causes DNA damage that strongly activates p53. This is a well-characterized p53-activating stress.
Reason: Core stress response function. UV-induced DNA damage is a classic p53 activator leading to repair, arrest, or apoptosis.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type
GO:0009651 response to salt stress
IEA
GO_REF:0000107 		REMOVE
Summary: Salt stress response is not a well-characterized p53 function in mammalian cells. This may be an over-annotation.
Reason: No strong evidence for salt stress as a p53-activating stimulus in mammalian cells. Likely computational over-annotation.
Supporting Evidence:
TP53-deep-research
p53 serves as a central node in the DNA damage response network ... It integrates signals from upstream kinases (ATM, ATR, DNA-PK) [no mention of salt stress as an activating signal]
GO:0009792 embryo development ending in birth or egg hatching
IEA
GO_REF:0000107 		KEEP AS NON CORE
Summary: Very broad developmental term. p53-null mice are viable, so p53 is not essential for embryo development to birth.
Reason: While p53 has developmental roles, it is not essential for embryo development to birth. This is a non-core function.
GO:0010165 response to X-ray
IEA
GO_REF:0000107 		ACCEPT
Summary: X-ray radiation causes DNA damage that activates p53. Part of p53s DNA damage response function.
Reason: Core function - ionizing radiation like X-rays cause DNA damage that strongly activates p53.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type
GO:0010332 response to gamma radiation
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 responds to gamma radiation-induced DNA damage.
Reason: Core function - ionizing radiation strongly activates p53.
Supporting Evidence:
TP53-deep-research
p53 responds to DNA damage from ionizing radiation
PMID:7958916
DNA damage triggers a prolonged p53-dependent G1 arrest and long-term induction of Cip1 in normal human fibroblasts.
GO:0010629 negative regulation of gene expression
IEA
GO_REF:0000107 		ACCEPT
Summary: TP53 functions as both activator and repressor of gene expression. It represses anti-apoptotic and proliferation genes.
Reason: Core transcriptional function. p53 represses multiple genes including BCL2, survivin, and cell cycle genes.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
repression of Bcl-2 expression
GO:0010659 cardiac muscle cell apoptotic process
IEA
GO_REF:0000107 		KEEP AS NON CORE
Summary: Cell type-specific apoptosis. While p53 induces apoptosis broadly, cardiac-specific regulation is context-dependent.
Reason: Tissue-specific manifestation of p53s apoptotic function. Not a core function but a specialized context.
GO:0010666 positive regulation of cardiac muscle cell apoptotic process
IEA
GO_REF:0000107 		KEEP AS NON CORE
Summary: Tissue-specific apoptotic regulation. p53 can induce apoptosis in cardiac cells but this is not a core function.
Reason: Cell type-specific apoptotic function. The general apoptotic function is core, but cardiac-specific regulation is context-dependent.
GO:0014009 glial cell proliferation
IEA
GO_REF:0000107 		KEEP AS NON CORE
Summary: Cell type-specific proliferation. Not a core p53 function.
Reason: Tissue-specific proliferation regulation in nervous system. Not central to p53 tumor suppressor function.
GO:0019661 glucose catabolic process to lactate via pyruvate
IEA
GO_REF:0000107 		MODIFY
Summary: TP53 regulates glucose metabolism and suppresses glycolysis (Warburg effect). There is also annotation for negative regulation (GO:1904024).
Reason: p53 primarily SUPPRESSES glycolysis/Warburg effect, not promotes it. The negative regulation annotation is more accurate.
Supporting Evidence:
TP53-deep-research
Wild-type p53: Inhibits glycolysis, promotes oxidative phosphorylation and mitochondrial respiration ... Specific targets: TIGAR (inhibits glycolysis), SCO2 (promotes oxidative phosphorylation)
GO:0021549 cerebellum development
IEA
GO_REF:0000107 		KEEP AS NON CORE
Summary: Specific brain region development. Not essential as p53-null mice have functional cerebellum.
Reason: Region-specific developmental process that is not core to p53 function.
GO:0030330 DNA damage response, signal transduction by p53 class mediator
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 mediates DNA damage response signal transduction as THE p53 class mediator.
Reason: Absolutely core function - p53 is the defining member of the p53 class mediator family.
Supporting Evidence:
TP53-deep-research
p53 serves as a central node in the DNA damage response network
PMID:29681526
A Designed Peptide Targets Two Types of Modifications of p53 with Anti-cancer Activity.
PMID:15149599
Telomere shortening triggers senescence of human cells through a pathway involving ATM, p53, and p21(CIP1), but not p16(INK4a).
PMID:20160708
Feedback between p21 and reactive oxygen production is necessary for cell senescence.
PMID:7958916
DNA damage triggers a prolonged p53-dependent G1 arrest and long-term induction of Cip1 in normal human fibroblasts.
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:16213212
Regulation of p53 translation and induction after DNA damage by ribosomal protein L26 and nucleolin.
GO:0030512 negative regulation of transforming growth factor beta receptor signaling pathway
IEA
GO_REF:0000107 		KEEP AS NON CORE
Summary: p53 has complex crosstalk with TGF-beta signaling but this is not a core function.
Reason: Context-specific signaling crosstalk rather than core p53 function.
GO:0031571 mitotic G1 DNA damage checkpoint signaling
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 mediates G1 DNA damage checkpoint signaling through p21 induction.
Reason: Core function - G1/S checkpoint is a major p53-mediated cell cycle control.
Supporting Evidence:
TP53-deep-research
Cell cycle arrest is mediated by transcriptional upregulation of p21
PMID:7958916
DNA damage triggers a prolonged p53-dependent G1 arrest and long-term induction of Cip1 in normal human fibroblasts.
GO:0033077 T cell differentiation in thymus
IEA
GO_REF:0000107 		KEEP AS NON CORE
Summary: Specific immune system developmental process. Not a core p53 function.
Reason: Tissue-specific developmental process in immune system. Not central to p53 tumor suppressor function.
GO:0033554 cellular response to stress
IEA
GO_REF:0000107 		ACCEPT
Summary: Broad term encompassing p53s fundamental role as a stress sensor and responder. Covers DNA damage, oxidative stress, hypoxia, etc.
Reason: Core function - p53 is the master cellular stress response coordinator, responding to diverse stress signals.
Supporting Evidence:
PMID:14744935
The tumor suppressor p53, a sensor of multiple forms of cellular stress, is regulated by post-translational mechanisms to induce cell-cycle arrest, senescence, or apoptosis
GO:0034103 regulation of tissue remodeling
IEA
GO_REF:0000107 		KEEP AS NON CORE
Summary: Tissue remodeling is not a well-characterized p53 function. This appears to be an over-annotation.
Reason: Not a core p53 function. May relate to indirect effects through apoptosis or senescence but not a primary role.
GO:0035264 multicellular organism growth
IEA
GO_REF:0000107 		KEEP AS NON CORE
Summary: Very broad developmental term. p53 influences growth through proliferation control but is not essential for organism growth.
Reason: Broad developmental process. p53-null mice show normal growth patterns despite cancer susceptibility.
GO:0035794 positive regulation of mitochondrial membrane permeability
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 promotes mitochondrial outer membrane permeabilization during apoptosis through BAX/BAK activation. Core apoptotic function.
Reason: Core component of p53-mediated intrinsic apoptosis. p53 target genes like BAX and PUMA promote MOMP.
Supporting Evidence:
GO:1902108
regulation of mitochondrial membrane permeability involved in apoptotic process
GO:0042127 regulation of cell population proliferation
IEA
GO_REF:0000107 		ACCEPT
Summary: General term for proliferation regulation. p53 primarily negatively regulates proliferation through cell cycle arrest.
Reason: Core function - p53 is a key regulator of proliferation, primarily through negative regulation via cell cycle checkpoints.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Negatively regulates cell division
GO:0043065 positive regulation of apoptotic process
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 positively regulates apoptosis as a major tumor suppressor mechanism.
Reason: Core function - p53 induces apoptosis through multiple pathways.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression
PMID:12667443
p53 has a direct apoptogenic role at the mitochondria.
PMID:15565177
A novel mitochondrial protein DIP mediates E2F1-induced apoptosis independently of p53.
PMID:20959462
2010 Oct 19. Aurora B interacts with NIR-p53, leading to p53 phosphorylation in its DNA-binding domain and subsequent functional suppression.
GO:0043066 negative regulation of apoptotic process
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 can negatively regulate apoptosis in certain contexts through p21-mediated survival.
Reason: Context-dependent function - p53 can promote survival under mild stress conditions.
Supporting Evidence:
PMID:14744935
We demonstrate that endoplasmic reticulum (ER) stress inhibits p53-mediated apoptosis
GO:0043504 mitochondrial DNA repair
IEA
GO_REF:0000107 		KEEP AS NON CORE
Summary: p53 has mitochondrial localization and can contribute to mitochondrial genome stability, though this is not a primary function.
Reason: While p53 can localize to mitochondria and influence mtDNA stability, this is not a core tumor suppressor function.
GO:0043516 regulation of DNA damage response, signal transduction by p53 class mediator
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 both executes and regulates its own DNA damage response pathway. This is a core function.
Reason: Core function - p53 is the central regulator of its own pathway, with multiple feedback loops and regulatory mechanisms.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type
GO:0043523 regulation of neuron apoptotic process
IEA
GO_REF:0000107 		KEEP AS NON CORE
Summary: Cell type-specific apoptotic regulation. p53 can regulate neuronal apoptosis but this is context-specific.
Reason: Tissue-specific manifestation of p53s apoptotic function. Not a core function but relevant in neurodegeneration contexts.
GO:0043525 positive regulation of neuron apoptotic process
IEA
GO_REF:0000107 		KEEP AS NON CORE
Summary: Neuron-specific pro-apoptotic function. While p53 induces apoptosis broadly, neuron-specific regulation is context-dependent.
Reason: Cell type-specific apoptotic function relevant in neurodegeneration but not a core p53 function.
GO:0045861 negative regulation of proteolysis
IEA
GO_REF:0000107 		KEEP AS NON CORE
Summary: p53 can regulate proteolysis through various mechanisms including MDM2 regulation and proteasome activity, but this is not a primary function.
Reason: While p53 influences protein stability and degradation pathways, negative regulation of proteolysis is not a core function.
GO:0045893 positive regulation of DNA-templated transcription
IEA
GO_REF:0000120 		ACCEPT
Summary: p53 positively regulates DNA-templated transcription of target genes.
Reason: Core transcriptional function - p53 activates transcription of numerous target genes.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:20378837
Glutaminase 2, a novel p53 target gene regulating energy metabolism and antioxidant function.
PMID:16322561
NIR is a novel INHAT repressor that modulates the transcriptional activity of p53.
PMID:17403783
HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53.
PMID:20096447
USP10 regulates p53 localization and stability by deubiquitinating p53.
GO:0045930 negative regulation of mitotic cell cycle
IEA
GO_REF:0000107 		ACCEPT
Summary: Core function - p53 induces cell cycle arrest at multiple checkpoints to prevent proliferation of damaged cells.
Reason: Fundamental tumor suppressor function. p53 arrests cell cycle through p21 and other CDK inhibitors.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Negatively regulates cell division by controlling expression of a set of genes required for this process
GO:0045944 positive regulation of transcription by RNA polymerase II
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 is a sequence-specific transcriptional activator that upregulates target genes including p21, PUMA, BAX.
Reason: Fundamental molecular function - p53 activates transcription of numerous target genes through binding to p53 response elements.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:16061649
Ckap2 regulates aneuploidy, cell cycling, and cell death in a p53-dependent manner.
PMID:17599062
Jun 28. CDIP, a novel pro-apoptotic gene, regulates TNFalpha-mediated apoptosis in a p53-dependent manner.
PMID:30089260
p53 Regulates the Expression of LRP1 and Apoptosis through a Stress Intensity-Dependent MicroRNA Feedback Loop.
PMID:7958916
DNA damage triggers a prolonged p53-dependent G1 arrest and long-term induction of Cip1 in normal human fibroblasts.
PMID:11672523
hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase.
PMID:17145718
Brn-3b enhances the pro-apoptotic effects of p53 but not its induction of cell cycle arrest by cooperating in trans-activation of bax expression.
PMID:17146433
Dec 4. RGC32, a novel p53-inducible gene, is located on centrosomes during mitosis and results in G2/M arrest.
PMID:18549481
TATA binding protein associated factor 3 (TAF3) interacts with p53 and inhibits its function.
PMID:24356969
2013 Dec 19. Rbm24, an RNA-binding protein and a target of p53, regulates p21 expression via mRNA stability.
PMID:17310983
Feb 19. Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function.
PMID:24652652
2014 Mar 20. DRAGO (KIAA0247), a new DNA damage-responsive, p53-inducible gene that cooperates with p53 as oncosuppressor.
PMID:20959462
2010 Oct 19. Aurora B interacts with NIR-p53, leading to p53 phosphorylation in its DNA-binding domain and subsequent functional suppression.
GO:0048144 fibroblast proliferation
IEA
GO_REF:0000107 		REMOVE
Summary: Cell type-specific proliferation. p53 generally inhibits rather than promotes proliferation.
Reason: p53 primarily inhibits proliferation. This positive proliferation annotation appears incorrect. The negative regulation annotation (GO:0048147) is accurate.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Negatively regulates cell division
GO:0048147 negative regulation of fibroblast proliferation
IEA
GO_REF:0000107 		KEEP AS NON CORE
Summary: p53 negatively regulates fibroblast proliferation as part of its anti-proliferative function.
Reason: Cell type-specific manifestation of anti-proliferative function.
GO:0048568 embryonic organ development
IEA
GO_REF:0000107 		KEEP AS NON CORE
Summary: Broad developmental term. p53 has developmental roles but is not essential for organ development.
Reason: Developmental process that is not core to p53 function. p53-null mice develop functional organs.
GO:0050821 protein stabilization
IEA
GO_REF:0000107 		KEEP AS NON CORE
Summary: p53 can stabilize some proteins through transcriptional targets or protein interactions, but this is not a primary function.
Reason: Not a core p53 function. p53 itself is heavily regulated by stability but protein stabilization of other proteins is not primary.
GO:0051276 chromosome organization
IEA
GO_REF:0000107 		REMOVE
Summary: Very broad term. p53 influences chromosome stability through DNA repair and checkpoints but does not directly organize chromosomes.
Reason: Too broad and indirect. p53 maintains genome stability but chromosome organization per se is not its function.
Supporting Evidence:
TP53-deep-research
p53 serves as a central node in the DNA damage response network ... Cell cycle checkpoint activation; DNA repair pathway stimulation [maintains genome stability but not chromosome organization per se]
GO:0051402 neuron apoptotic process
IEA
GO_REF:0000107 		KEEP AS NON CORE
Summary: Neuron-specific apoptosis. While p53 can induce neuronal apoptosis, this is context-specific.
Reason: Cell type-specific apoptotic process. Relevant in neurodegeneration but not a core p53 function.
GO:0051726 regulation of cell cycle
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 is a master regulator of the cell cycle, inducing arrest at G1/S and G2/M checkpoints.
Reason: Core function - cell cycle regulation is fundamental to p53's tumor suppressor activity.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
induces cell cycle arrest
PMID:30514107
CELF1/p53 axis: a sustained antiproliferative signal leading to villus atrophy under total parenteral nutrition.
PMID:12203124
The Brn-3a transcription factor inhibits the pro-apoptotic effect of p53 and enhances cell cycle arrest by differentially regulating the activity of the p53 target genes encoding Bax and p21(CIP1/Waf1).
PMID:12433990
Distinct promoter elements mediate the co-operative effect of Brn-3a and p53 on the p21 promoter and their antagonism on the Bax promoter.
PMID:15149599
Telomere shortening triggers senescence of human cells through a pathway involving ATM, p53, and p21(CIP1), but not p16(INK4a).
PMID:7958916
DNA damage triggers a prolonged p53-dependent G1 arrest and long-term induction of Cip1 in normal human fibroblasts.
GO:0060253 negative regulation of glial cell proliferation
IEA
GO_REF:0000107 		KEEP AS NON CORE
Summary: Cell type-specific anti-proliferative function in nervous system.
Reason: Tissue-specific manifestation of p53s anti-proliferative function. Not a core function.
GO:0060411 cardiac septum morphogenesis
IEA
GO_REF:0000107 		KEEP AS NON CORE
Summary: Very specific cardiac developmental process. Not a core p53 function.
Reason: Highly specific developmental process. p53-null mice have normal cardiac development.
GO:0070242 thymocyte apoptotic process
IEA
GO_REF:0000107 		KEEP AS NON CORE
Summary: Cell type-specific apoptosis in immune system development.
Reason: Tissue-specific apoptotic process in immune system. Not a core p53 function.
GO:0070243 regulation of thymocyte apoptotic process
IEA
GO_REF:0000107 		KEEP AS NON CORE
Summary: Immune cell-specific apoptotic regulation during T cell development.
Reason: Tissue-specific process in immune system development. Not a core p53 function.
GO:0070266 necroptotic process
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 has emerging roles in regulating necroptosis, a form of programmed necrosis, though apoptosis is its primary death pathway.
Reason: Emerging function of p53 in non-apoptotic cell death. p53 can regulate necroptosis through various mechanisms.
GO:0071480 cellular response to gamma radiation
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 responds to gamma radiation-induced DNA damage.
Reason: Core function - ionizing radiation is a classic p53 activator.
Supporting Evidence:
TP53-deep-research
gamma radiation causes DNA damage that strongly activates p53
PMID:16213212
Regulation of p53 translation and induction after DNA damage by ribosomal protein L26 and nucleolin.
GO:0071494 cellular response to UV-C
IEA
GO_REF:0000107 		ACCEPT
Summary: UV-C radiation causes severe DNA damage that activates p53. Part of DNA damage response.
Reason: Core function - UV-C induces DNA lesions that strongly activate p53-mediated responses.
Supporting Evidence:
GO:0009411
response to UV
GO:0072089 stem cell proliferation
IEA
GO_REF:0000107 		REMOVE
Summary: p53 generally suppresses rather than promotes proliferation, including in stem cells.
Reason: p53 typically inhibits stem cell proliferation to maintain genomic stability. The negative regulation annotation (GO:2000647) is more accurate.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Negatively regulates cell division by controlling expression of a set of genes required for this process
GO:0072331 signal transduction by p53 class mediator
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 IS the p53 class mediator that transduces signals in response to stress.
Reason: Absolutely core function - this term specifically describes p53's central role in stress signaling.
Supporting Evidence:
TP53-deep-research
p53 coordinates cellular responses to diverse stress signals
PMID:25384516
Rad54B serves as a scaffold in the DNA damage response that limits checkpoint strength.
PMID:18549481
TATA binding protein associated factor 3 (TAF3) interacts with p53 and inhibits its function.
PMID:29681526
A Designed Peptide Targets Two Types of Modifications of p53 with Anti-cancer Activity.
PMID:15314173
Ribosomal protein L23 activates p53 by inhibiting MDM2 function in response to ribosomal perturbation but not to translation inhibition.
GO:0072332 intrinsic apoptotic signaling pathway by p53 class mediator
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 mediates intrinsic apoptotic signaling through BAX, PUMA, NOXA activation.
Reason: Core apoptotic function - p53 is the master regulator of intrinsic apoptosis.
Supporting Evidence:
TP53-deep-research
Transcriptional: Activation of pro-apoptotic genes (PUMA, BAX, NOXA)
PMID:17310983
Feb 19. Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function.
PMID:16322561
NIR is a novel INHAT repressor that modulates the transcriptional activity of p53.
PMID:12172011
Chk2 regulates irradiation-induced, p53-mediated apoptosis in Drosophila.
GO:0072593 reactive oxygen species metabolic process
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 regulates ROS through multiple mechanisms including antioxidant genes and metabolic regulation. Important for redox balance.
Reason: Important function - p53 regulates cellular redox state through multiple target genes affecting ROS production and scavenging.
Supporting Evidence:
GO:2000378
negative regulation of reactive oxygen species metabolic process
GO:1901525 negative regulation of mitophagy
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 has complex bidirectional effects on autophagy/mitophagy - can both promote and inhibit depending on context.
Reason: p53 can negatively regulate mitophagy in certain contexts while promoting it in others. This dual role is well-documented.
GO:1902108 regulation of mitochondrial membrane permeability involved in apoptotic process
IEA
GO_REF:0000107 		ACCEPT
Summary: Core apoptotic function - p53 regulates mitochondrial outer membrane permeabilization through BAX/BAK activation.
Reason: Core function in intrinsic apoptosis. p53 target genes like BAX and PUMA promote mitochondrial membrane permeabilization.
Supporting Evidence:
GO:0035794
positive regulation of mitochondrial membrane permeability
GO:1902253 regulation of intrinsic apoptotic signaling pathway by p53 class mediator
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 both executes and regulates its own intrinsic apoptotic pathway. Core function.
Reason: Core function - p53 is the master regulator of its own apoptotic pathway with multiple feedback mechanisms.
Supporting Evidence:
GO:0072332
intrinsic apoptotic signaling pathway by p53 class mediator
GO:1903799 negative regulation of miRNA processing
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 has complex effects on miRNA processing - can both promote and inhibit. There is also positive regulation annotation (GO:1902895).
Reason: p53 regulates miRNA processing through interaction with Drosha complex and other mechanisms. Can have both positive and negative effects.
GO:1904024 negative regulation of glucose catabolic process to lactate via pyruvate
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 suppresses glycolysis/Warburg effect as part of its metabolic regulatory and tumor suppressor functions.
Reason: Important metabolic function - p53 suppresses aerobic glycolysis (Warburg effect) that is characteristic of cancer cells.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
GO; GO:0019661; P:glucose catabolic process to lactate via pyruvate; IEA:Ensembl ... GO; GO:1904024; P:negative regulation of glucose catabolic process to lactate via pyruvate; IEA:Ensembl
GO:1990144 intrinsic apoptotic signaling pathway in response to hypoxia
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 mediates apoptosis in response to severe hypoxia. Part of its stress response repertoire.
Reason: Core stress response function - p53 induces apoptosis under severe hypoxic stress through HIF interactions and target genes.
Supporting Evidence:
GO:0002931
response to ischemia
GO:2000269 regulation of fibroblast apoptotic process
IEA
GO_REF:0000107 		KEEP AS NON CORE
Summary: Cell type-specific apoptotic regulation in fibroblasts.
Reason: Tissue-specific apoptotic function. The general apoptotic function is core, but fibroblast-specific regulation is context-dependent.
GO:2000378 negative regulation of reactive oxygen species metabolic process
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 regulates cellular redox balance and can suppress ROS through antioxidant gene expression.
Reason: Important function - p53 maintains redox homeostasis by inducing antioxidant genes like GPX1, SOD2, and others.
Supporting Evidence:
GO:0072593
reactive oxygen species metabolic process
GO:2000647 negative regulation of stem cell proliferation
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 maintains stem cell genomic stability by limiting proliferation. Important for preventing cancer stem cells.
Reason: Important function - p53 restricts stem cell self-renewal to maintain genomic integrity and prevent transformation.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
induces growth arrest or apoptosis depending on the physiological circumstances and cell type ... Negatively regulates cell division by controlling expression of a set of genes required for this process
GO:2000772 regulation of cellular senescence
IEA
GO_REF:0000107 		ACCEPT
Summary: Senescence induction is a core p53 tumor suppressor mechanism, alternative to apoptosis for preventing damaged cell proliferation.
Reason: Core function - p53 induces senescence through p21 and other targets as an irreversible cell cycle arrest mechanism.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence ... Negatively regulates cell division
GO:0160020 positive regulation of ferroptosis
TAS
file:human/TP53/TP53-deep-research.md 		NEW
Summary: p53 promotes ferroptosis through multiple mechanisms including repression of SLC7A11 (cystine/glutamate antiporter), activation of ALOX12, and promotion of lipid peroxidation. This is an important non-apoptotic cell death mechanism.
Reason: Ferroptosis regulation is a well-established p53 function documented in recent literature but missing from current GO annotations. p53 acts as a positive regulator of ferroptosis.
Supporting Evidence:
TP53-deep-research
Pro-ferroptotic: Represses SLC7A11, activates ALOX12, promotes lipid peroxidation
TP53-deep-research
p53 has a dual role in ferroptosis (iron-dependent programmed cell death) ... p53 act as positive regulators of ferroptosis by promotion of ROS production and inhibition of expression of SLC7A11
GO:1902895 positive regulation of miRNA transcription
IMP
PMID:30089260
p53 Regulates the Expression of LRP1 and Apoptosis through a... 		ACCEPT
Summary: p53 transcriptionally regulates multiple miRNAs including miR-34 family.
Reason: p53 regulates miRNA expression as part of its stress response functions.
Supporting Evidence:
PMID:30089260
we only observed significant Dox dose- and time-dependent increases in the expression levels of miR-103 and miR-107... we found p53-dependent induction of these miRNAs upon nutlin-3a treatment
PMID:30089260
Using our CRISPR p53KO HCT116 cell lines, we found p53-dependent induction of these miRNAs upon nutlin-3a treatment
GO:0008285 negative regulation of cell population proliferation
ISS
PMID:30514107
CELF1/p53 axis: a sustained antiproliferative signal leading... 		ACCEPT
Summary: p53 negatively regulates cell proliferation as a fundamental tumor suppressor mechanism.
Reason: Core function - p53 inhibits proliferation through cell cycle arrest.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Negatively regulates cell division
PMID:30514107
CELF1/p53 axis: a sustained antiproliferative signal leading to villus atrophy under total parenteral nutrition.
PMID:12730672
Physical and functional interaction between HCV core protein and the different p73 isoforms.
PMID:22783376
Induction of apoptosis by cytoplasmically localized wild-type p53 and the S121F mutant super p53.
GO:0051726 regulation of cell cycle
ISS
PMID:30514107
CELF1/p53 axis: a sustained antiproliferative signal leading... 		ACCEPT
Summary: p53 is a master regulator of the cell cycle, inducing arrest at G1/S and G2/M checkpoints.
Reason: Core function - cell cycle regulation is fundamental to p53's tumor suppressor activity.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
induces cell cycle arrest
PMID:30514107
CELF1/p53 axis: a sustained antiproliferative signal leading to villus atrophy under total parenteral nutrition.
PMID:12203124
The Brn-3a transcription factor inhibits the pro-apoptotic effect of p53 and enhances cell cycle arrest by differentially regulating the activity of the p53 target genes encoding Bax and p21(CIP1/Waf1).
PMID:12433990
Distinct promoter elements mediate the co-operative effect of Brn-3a and p53 on the p21 promoter and their antagonism on the Bax promoter.
PMID:15149599
Telomere shortening triggers senescence of human cells through a pathway involving ATM, p53, and p21(CIP1), but not p16(INK4a).
PMID:7958916
DNA damage triggers a prolonged p53-dependent G1 arrest and long-term induction of Cip1 in normal human fibroblasts.
GO:1902749 regulation of cell cycle G2/M phase transition
IMP
PMID:10962037
Overexpression of MYC causes p53-dependent G2 arrest of norm... 		ACCEPT
Summary: p53 regulates the G2/M checkpoint, preventing mitosis of damaged cells. Core cell cycle control function.
Reason: Core cell cycle checkpoint function with experimental evidence. p53 induces G2 arrest through multiple mechanisms.
Supporting Evidence:
PMID:10962037
Overexpression of MYC causes p53-dependent G2 arrest of normal fibroblasts
GO:0045944 positive regulation of transcription by RNA polymerase II
IGI
PMID:16061649
Ckap2 regulates aneuploidy, cell cycling, and cell death in ... 		ACCEPT
Summary: p53 is a sequence-specific transcriptional activator that upregulates target genes including p21, PUMA, BAX.
Reason: Fundamental molecular function - p53 activates transcription of numerous target genes through binding to p53 response elements.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:16061649
Ckap2 regulates aneuploidy, cell cycling, and cell death in a p53-dependent manner.
PMID:17599062
Jun 28. CDIP, a novel pro-apoptotic gene, regulates TNFalpha-mediated apoptosis in a p53-dependent manner.
PMID:30089260
p53 Regulates the Expression of LRP1 and Apoptosis through a Stress Intensity-Dependent MicroRNA Feedback Loop.
PMID:7958916
DNA damage triggers a prolonged p53-dependent G1 arrest and long-term induction of Cip1 in normal human fibroblasts.
PMID:11672523
hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase.
PMID:17145718
Brn-3b enhances the pro-apoptotic effects of p53 but not its induction of cell cycle arrest by cooperating in trans-activation of bax expression.
PMID:17146433
Dec 4. RGC32, a novel p53-inducible gene, is located on centrosomes during mitosis and results in G2/M arrest.
PMID:18549481
TATA binding protein associated factor 3 (TAF3) interacts with p53 and inhibits its function.
PMID:24356969
2013 Dec 19. Rbm24, an RNA-binding protein and a target of p53, regulates p21 expression via mRNA stability.
PMID:17310983
Feb 19. Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function.
PMID:24652652
2014 Mar 20. DRAGO (KIAA0247), a new DNA damage-responsive, p53-inducible gene that cooperates with p53 as oncosuppressor.
PMID:20959462
2010 Oct 19. Aurora B interacts with NIR-p53, leading to p53 phosphorylation in its DNA-binding domain and subsequent functional suppression.
GO:0000976 transcription cis-regulatory region binding
IDA
PMID:15710329
Human MUC1 oncoprotein regulates p53-responsive gene transcr... 		ACCEPT
Summary: Direct experimental evidence for p53 DNA binding activity. Core molecular function with IDA support.
Reason: Core molecular function with direct experimental evidence of p53 binding to cis-regulatory regions.
Supporting Evidence:
PMID:15710329
Human MUC1 oncoprotein regulates p53-responsive gene transcription in the genotoxic stress response
GO:0042771 intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
IDA
PMID:14654789
A member of the Pyrin family, IFI16, is a novel BRCA1-associ... 		ACCEPT
Summary: p53 is THE p53 class mediator that induces intrinsic apoptosis in response to DNA damage through BAX, PUMA, NOXA activation.
Reason: Absolutely core function - this term specifically describes p53's role as the central mediator of DNA damage-induced apoptosis.
Supporting Evidence:
TP53-deep-research
Transcriptional: Activation of pro-apoptotic genes (PUMA, BAX, NOXA, FAS)
PMID:14654789
A member of the Pyrin family, IFI16, is a novel BRCA1-associated protein involved in the p53-mediated apoptosis pathway.
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:16213212
Regulation of p53 translation and induction after DNA damage by ribosomal protein L26 and nucleolin.
PMID:17403783
HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53.
GO:0006974 DNA damage response
IDA
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localiz... 		ACCEPT
Summary: p53 is central to the DNA damage response, being activated by and coordinating responses to DNA damage.
Reason: Core function - p53 is the 'guardian of the genome' that coordinates DNA damage responses.
Supporting Evidence:
TP53-deep-research
p53 serves as a central node in the DNA damage response network
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:30089260
p53 Regulates the Expression of LRP1 and Apoptosis through a Stress Intensity-Dependent MicroRNA Feedback Loop.
PMID:24356969
2013 Dec 19. Rbm24, an RNA-binding protein and a target of p53, regulates p21 expression via mRNA stability.
PMID:17938203
Oct 15. Protein kinase C delta induces transcription of the TP53 tumor suppressor gene by controlling death-promoting factor Btf in the apoptotic response to DNA damage.
PMID:15710329
Human MUC1 oncoprotein regulates p53-responsive gene transcription in the genotoxic stress response.
GO:0006983 ER overload response
IDA
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localiz... 		ACCEPT
Summary: p53 responds to ER stress. The cited paper shows ER stress affects p53 localization and function.
Reason: p53 responds to ER stress as part of its cellular stress response repertoire. Supported by experimental evidence.
Supporting Evidence:
PMID:14744935
endoplasmic reticulum (ER) stress inhibits p53-mediated apoptosis... ER stress induces GSK-3beta binding to p53
GO:0042771 intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
IDA
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localiz... 		ACCEPT
Summary: p53 is THE p53 class mediator that induces intrinsic apoptosis in response to DNA damage through BAX, PUMA, NOXA activation.
Reason: Absolutely core function - this term specifically describes p53's role as the central mediator of DNA damage-induced apoptosis.
Supporting Evidence:
TP53-deep-research
Transcriptional: Activation of pro-apoptotic genes (PUMA, BAX, NOXA, FAS)
PMID:14654789
A member of the Pyrin family, IFI16, is a novel BRCA1-associated protein involved in the p53-mediated apoptosis pathway.
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:16213212
Regulation of p53 translation and induction after DNA damage by ribosomal protein L26 and nucleolin.
PMID:17403783
HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53.
GO:0042981 regulation of apoptotic process
IDA
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localiz... 		ACCEPT
Summary: p53 is a master regulator of apoptosis, both promoting and (in some contexts) inhibiting apoptotic processes.
Reason: Core function - p53 regulates apoptosis through multiple mechanisms including transcriptional activation of pro-apoptotic genes.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
GO:0016604 nuclear body
IDA
PMID:10360174
Mutations in human ARF exon 2 disrupt its nucleolar localiza... 		ACCEPT
Summary: p53 localizes to nuclear bodies including PML bodies and nucleoli under certain conditions. This is related to its regulatory functions.
Reason: p53 localizes to nuclear bodies like PML bodies, especially during stress responses and senescence. This subcellular localization is functionally relevant.
Supporting Evidence:
PMID:10360174
ARF exon 2 disrupt its nucleolar localization and impair its ability to block nuclear export of MDM2 and p53
GO:0000976 transcription cis-regulatory region binding
IDA
PMID:17996705
An acetylation switch in p53 mediates holo-TFIID recruitment... 		ACCEPT
Summary: Additional IDA evidence for p53 DNA binding. Duplicate annotation with different experimental support.
Reason: Core molecular function with direct experimental evidence. p53 binds to consensus sequences in target gene promoters.
Supporting Evidence:
PMID:17996705
An acetylation switch in p53 mediates holo-TFIID recruitment
GO:0045944 positive regulation of transcription by RNA polymerase II
IDA
PMID:17599062
CDIP, a novel pro-apoptotic gene, regulates TNFalpha-mediate... 		ACCEPT
Summary: p53 is a sequence-specific transcriptional activator that upregulates target genes including p21, PUMA, BAX.
Reason: Fundamental molecular function - p53 activates transcription of numerous target genes through binding to p53 response elements.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:16061649
Ckap2 regulates aneuploidy, cell cycling, and cell death in a p53-dependent manner.
PMID:17599062
Jun 28. CDIP, a novel pro-apoptotic gene, regulates TNFalpha-mediated apoptosis in a p53-dependent manner.
PMID:30089260
p53 Regulates the Expression of LRP1 and Apoptosis through a Stress Intensity-Dependent MicroRNA Feedback Loop.
PMID:7958916
DNA damage triggers a prolonged p53-dependent G1 arrest and long-term induction of Cip1 in normal human fibroblasts.
PMID:11672523
hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase.
PMID:17145718
Brn-3b enhances the pro-apoptotic effects of p53 but not its induction of cell cycle arrest by cooperating in trans-activation of bax expression.
PMID:17146433
Dec 4. RGC32, a novel p53-inducible gene, is located on centrosomes during mitosis and results in G2/M arrest.
PMID:18549481
TATA binding protein associated factor 3 (TAF3) interacts with p53 and inhibits its function.
PMID:24356969
2013 Dec 19. Rbm24, an RNA-binding protein and a target of p53, regulates p21 expression via mRNA stability.
PMID:17310983
Feb 19. Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function.
PMID:24652652
2014 Mar 20. DRAGO (KIAA0247), a new DNA damage-responsive, p53-inducible gene that cooperates with p53 as oncosuppressor.
PMID:20959462
2010 Oct 19. Aurora B interacts with NIR-p53, leading to p53 phosphorylation in its DNA-binding domain and subsequent functional suppression.
GO:0042149 cellular response to glucose starvation
IDA
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localiz... 		ACCEPT
Summary: p53 responds to glucose starvation as part of metabolic stress response.
Reason: p53 responds to metabolic stress including glucose deprivation.
Supporting Evidence:
PMID:14744935
The tumor suppressor p53, a sensor of multiple forms of cellular stress, is regulated by post-translational mechanisms to induce cell-cycle arrest, senescence, or apoptosis
GO:0001666 response to hypoxia
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 is activated by hypoxic stress and mediates cellular responses to low oxygen through HIF pathway interactions.
Reason: Core stress response function. Hypoxia is a well-established p53-activating stress that leads to cell cycle arrest or apoptosis depending on severity.
Supporting Evidence:
TP53-deep-research
p53 coordinates cellular responses to diverse stress signals including DNA damage, oxidative stress, hypoxia, and metabolic stress
GO:0000785 chromatin
IBA
GO_REF:0000033 		ACCEPT
Summary: p53 associates with chromatin at target gene promoters to regulate transcription.
Reason: Core molecular function - p53 binds to chromatin at p53 response elements to regulate target gene expression.
Supporting Evidence:
PDB:3TS8
Crystal structure of a multidomain human p53 tetramer bound to the natural CDKN1A(p21) p53-response element
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:30089260
p53 Regulates the Expression of LRP1 and Apoptosis through a Stress Intensity-Dependent MicroRNA Feedback Loop.
PMID:23629966
Deacetylation of p53 induces autophagy by suppressing Bmf expression.
PMID:22521434
Hepatocyte growth factor (HGF) and 1,25-dihydroxyvitamin D together stimulate human bone marrow-derived stem cells toward the osteogenic phenotype by HGF-induced up-regulation of VDR.
PMID:15710329
Human MUC1 oncoprotein regulates p53-responsive gene transcription in the genotoxic stress response.
PMID:17805299
p53 is regulated by the lysine demethylase LSD1.
GO:0005634 nucleus
IBA
GO_REF:0000033 		ACCEPT
Summary: p53 is primarily a nuclear transcription factor, with nuclear localization essential for its function.
Reason: Core cellular component - p53's nuclear localization is essential for its transcription factor activity. Contains nuclear localization signals at residues 305-322 and 369-375.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Endoplasmic reticulum.
PMID:14963330
Direct activation of Bax by p53 mediates mitochondrial membrane permeabilization and apoptosis.
PMID:16322561
NIR is a novel INHAT repressor that modulates the transcriptional activity of p53.
PMID:36108750
Phase separation of p53 induced by its unstructured basic region and prevented by oncogenic mutations in tetramerization domain.
PMID:24625977
Ubiquitin-like (UBX)-domain-containing protein, UBXN2A, promotes cell death by interfering with the p53-Mortalin interactions in colon cancer cells.
PMID:26634371
Epub 2015 Dec 4. Structural studies of UBXN2A and mortalin interaction and the putative role of silenced UBXN2A in preventing response to chemotherapy.
PMID:21597459
2011 May 20. E3 ubiquitin ligase Hades negatively regulates the exonuclear function of p53.
PMID:19011621
Arginine methylation regulates the p53 response.
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:24101517
Mitochondrial disulfide relay mediates translocation of p53 and partitions its subcellular activity.
PMID:20810912
Hypoxia downregulates p53 but induces apoptosis and enhances expression of BAD in cultures of human syncytiotrophoblasts.
PMID:19234109
Induction of SOX4 by DNA damage is critical for p53 stabilization and function.
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:17403783
HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53.
PMID:20096447
USP10 regulates p53 localization and stability by deubiquitinating p53.
PMID:18756595
Prognostic significance of BMP and activin membrane-bound inhibitor in colorectal cancer.
PMID:16507995
Myosin VI is a mediator of the p53-dependent cell survival pathway.
PMID:7720704
Direct involvement of p53 in programmed cell death of oligodendrocytes.
GO:0042771 intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
IBA
GO_REF:0000033 		ACCEPT
Summary: p53 is THE p53 class mediator that induces intrinsic apoptosis in response to DNA damage through BAX, PUMA, NOXA activation.
Reason: Absolutely core function - this term specifically describes p53's role as the central mediator of DNA damage-induced apoptosis.
Supporting Evidence:
TP53-deep-research
Transcriptional: Activation of pro-apoptotic genes (PUMA, BAX, NOXA, FAS)
PMID:14654789
A member of the Pyrin family, IFI16, is a novel BRCA1-associated protein involved in the p53-mediated apoptosis pathway.
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:16213212
Regulation of p53 translation and induction after DNA damage by ribosomal protein L26 and nucleolin.
PMID:17403783
HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53.
GO:0042981 regulation of apoptotic process
IBA
GO_REF:0000033 		ACCEPT
Summary: p53 is a master regulator of apoptosis, both promoting and (in some contexts) inhibiting apoptotic processes.
Reason: Core function - p53 regulates apoptosis through multiple mechanisms including transcriptional activation of pro-apoptotic genes.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
GO:0045944 positive regulation of transcription by RNA polymerase II
IBA
GO_REF:0000033 		ACCEPT
Summary: p53 is a sequence-specific transcriptional activator that upregulates target genes including p21, PUMA, BAX.
Reason: Fundamental molecular function - p53 activates transcription of numerous target genes through binding to p53 response elements.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:16061649
Ckap2 regulates aneuploidy, cell cycling, and cell death in a p53-dependent manner.
PMID:17599062
Jun 28. CDIP, a novel pro-apoptotic gene, regulates TNFalpha-mediated apoptosis in a p53-dependent manner.
PMID:30089260
p53 Regulates the Expression of LRP1 and Apoptosis through a Stress Intensity-Dependent MicroRNA Feedback Loop.
PMID:7958916
DNA damage triggers a prolonged p53-dependent G1 arrest and long-term induction of Cip1 in normal human fibroblasts.
PMID:11672523
hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase.
PMID:17145718
Brn-3b enhances the pro-apoptotic effects of p53 but not its induction of cell cycle arrest by cooperating in trans-activation of bax expression.
PMID:17146433
Dec 4. RGC32, a novel p53-inducible gene, is located on centrosomes during mitosis and results in G2/M arrest.
PMID:18549481
TATA binding protein associated factor 3 (TAF3) interacts with p53 and inhibits its function.
PMID:24356969
2013 Dec 19. Rbm24, an RNA-binding protein and a target of p53, regulates p21 expression via mRNA stability.
PMID:17310983
Feb 19. Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function.
PMID:24652652
2014 Mar 20. DRAGO (KIAA0247), a new DNA damage-responsive, p53-inducible gene that cooperates with p53 as oncosuppressor.
PMID:20959462
2010 Oct 19. Aurora B interacts with NIR-p53, leading to p53 phosphorylation in its DNA-binding domain and subsequent functional suppression.
GO:1990841 promoter-specific chromatin binding
IBA
GO_REF:0000033 		ACCEPT
Summary: p53 binds to promoter-specific chromatin regions containing p53 response elements.
Reason: Core molecular function - p53 specifically binds to promoter regions of target genes through sequence-specific DNA binding.
Supporting Evidence:
PDB:3TS8
Crystal structure shows p53 binding to CDKN1A(p21) promoter response element
PMID:24652652
2014 Mar 20. DRAGO (KIAA0247), a new DNA damage-responsive, p53-inducible gene that cooperates with p53 as oncosuppressor.
PMID:20725088
2010 Aug 20. Primate-specific RFPL1 gene controls cell-cycle progression through cyclin B1/Cdc2 degradation.
PMID:24356969
2013 Dec 19. Rbm24, an RNA-binding protein and a target of p53, regulates p21 expression via mRNA stability.
GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific
IBA
GO_REF:0000033 		ACCEPT
Summary: p53 is a DNA-binding transcription factor that regulates RNA polymerase II transcription of target genes.
Reason: Core molecular function - p53 is a well-characterized sequence-specific transcription factor for RNA pol II.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:34381247
Epub 2021 Aug 11. The tumor suppressor folliculin inhibits lactate dehydrogenase A and regulates the Warburg effect.
PMID:35618207
Ser392 phosphorylation modulated a switch between p53 and transcriptional condensates.
PMID:36634798
Phosphorylation and specific DNA improved the incorporation ability of p53 into functional condensates.
PMID:38653238
2024 Apr 22. Alanyl-tRNA synthetase, AARS1, is a lactate sensor and lactyltransferase that lactylates p53 and contributes to tumorigenesis.
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:17310983
Feb 19. Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function.
PMID:24652652
2014 Mar 20. DRAGO (KIAA0247), a new DNA damage-responsive, p53-inducible gene that cooperates with p53 as oncosuppressor.
GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding
IBA
GO_REF:0000033 		ACCEPT
Summary: p53 binds sequence-specifically to p53 response elements in cis-regulatory regions of target genes.
Reason: Core molecular function - p53's DNA-binding domain binds to consensus p53 response elements (p53REs) with high specificity.
Supporting Evidence:
TP53-deep-research
TP53 is fundamentally a transcription factor that binds sequence-specifically to p53 response elements (p53REs) in target gene promoters
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:22578566
Apr 19. Oncosuppressive role of p53-induced miR-205 in triple negative breast cancer.
GO:0003677 DNA binding
IEA
GO_REF:0000120 		ACCEPT
Summary: p53 binds DNA through its central DNA-binding domain (residues 102-292) to p53 response elements.
Reason: Core molecular function - DNA binding is essential for p53's transcription factor activity.
Supporting Evidence:
TP53-deep-research
DNA-Binding Domain (DBD): Location: Residues 102-292; Structure: beta-sandwich core with DNA-binding loops
PMID:15358771
2004 Sep 9. Hsp90 regulates the activity of wild type p53 under physiological and elevated temperatures.
PMID:15629713
Binding of natively unfolded HIF-1alpha ODD domain to p53.
PMID:2144364
Transcriptional activation by wild-type but not transforming mutants of the p53 anti-oncogene.
GO:0003700 DNA-binding transcription factor activity
IEA
GO_REF:0000120 		ACCEPT
Summary: p53 is a well-characterized DNA-binding transcription factor that activates and represses target genes.
Reason: Core molecular function - p53 is one of the best-characterized transcription factors, regulating >500 target genes.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:26334721
2015 Sep 3. Jmjd5 functions as a regulator of p53 signaling during mouse embryogenesis.
PMID:18549481
TATA binding protein associated factor 3 (TAF3) interacts with p53 and inhibits its function.
PMID:7587074
Targets for transcriptional activation by wild-type p53: endogenous retroviral LTR, immunoglobulin-like promoter, and an internal promoter of the mdm2 gene.
GO:0005634 nucleus
IEA
GO_REF:0000120 		ACCEPT
Summary: p53 is primarily a nuclear transcription factor, with nuclear localization essential for its function.
Reason: Core cellular component - p53's nuclear localization is essential for its transcription factor activity. Contains nuclear localization signals at residues 305-322 and 369-375.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Endoplasmic reticulum.
PMID:14963330
Direct activation of Bax by p53 mediates mitochondrial membrane permeabilization and apoptosis.
PMID:16322561
NIR is a novel INHAT repressor that modulates the transcriptional activity of p53.
PMID:36108750
Phase separation of p53 induced by its unstructured basic region and prevented by oncogenic mutations in tetramerization domain.
PMID:24625977
Ubiquitin-like (UBX)-domain-containing protein, UBXN2A, promotes cell death by interfering with the p53-Mortalin interactions in colon cancer cells.
PMID:26634371
Epub 2015 Dec 4. Structural studies of UBXN2A and mortalin interaction and the putative role of silenced UBXN2A in preventing response to chemotherapy.
PMID:21597459
2011 May 20. E3 ubiquitin ligase Hades negatively regulates the exonuclear function of p53.
PMID:19011621
Arginine methylation regulates the p53 response.
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:24101517
Mitochondrial disulfide relay mediates translocation of p53 and partitions its subcellular activity.
PMID:20810912
Hypoxia downregulates p53 but induces apoptosis and enhances expression of BAD in cultures of human syncytiotrophoblasts.
PMID:19234109
Induction of SOX4 by DNA damage is critical for p53 stabilization and function.
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:17403783
HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53.
PMID:20096447
USP10 regulates p53 localization and stability by deubiquitinating p53.
PMID:18756595
Prognostic significance of BMP and activin membrane-bound inhibitor in colorectal cancer.
PMID:16507995
Myosin VI is a mediator of the p53-dependent cell survival pathway.
PMID:7720704
Direct involvement of p53 in programmed cell death of oligodendrocytes.
GO:0005737 cytoplasm
IEA
GO_REF:0000120 		ACCEPT
Summary: p53 localizes to cytoplasm as well as nucleus, with cytoplasmic localization important for regulation.
Reason: Core cellular component - p53 shuttles between nucleus and cytoplasm, with cytoplasmic localization important for MDM2-mediated regulation.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus. Cytoplasm.
PMID:24625977
Ubiquitin-like (UBX)-domain-containing protein, UBXN2A, promotes cell death by interfering with the p53-Mortalin interactions in colon cancer cells.
PMID:26634371
Epub 2015 Dec 4. Structural studies of UBXN2A and mortalin interaction and the putative role of silenced UBXN2A in preventing response to chemotherapy.
PMID:21597459
2011 May 20. E3 ubiquitin ligase Hades negatively regulates the exonuclear function of p53.
PMID:19011621
Arginine methylation regulates the p53 response.
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:23629966
Deacetylation of p53 induces autophagy by suppressing Bmf expression.
PMID:20810912
Hypoxia downregulates p53 but induces apoptosis and enhances expression of BAD in cultures of human syncytiotrophoblasts.
PMID:16131611
p53 isoforms can regulate p53 transcriptional activity.
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:20096447
USP10 regulates p53 localization and stability by deubiquitinating p53.
PMID:7720704
Direct involvement of p53 in programmed cell death of oligodendrocytes.
GO:0005759 mitochondrial matrix
IEA
GO_REF:0000120 		ACCEPT
Summary: p53 localizes to mitochondria where it can directly promote apoptosis through non-transcriptional mechanisms.
Reason: Important cellular localization - p53 can translocate to mitochondria and directly trigger apoptosis through BAX/BAK activation.
Supporting Evidence:
TP53-deep-research
Non-transcriptional: Direct mitochondrial translocation and interaction with BCL-2 family proteins
GO:0005783 endoplasmic reticulum
IEA
GO_REF:0000044 		ACCEPT
Summary: p53 localizes to endoplasmic reticulum where it responds to ER stress.
Reason: p53 responds to ER stress signals and can localize to ER during stress responses.
Supporting Evidence:
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization
GO:0005813 centrosome
IEA
GO_REF:0000044 		KEEP AS NON CORE
Summary: p53 has been reported to localize to centrosomes but this is not a primary localization.
Reason: Non-core localization. p53 may associate with centrosomes in certain contexts but this is not a primary function.
GO:0006355 regulation of DNA-templated transcription
IEA
GO_REF:0000120 		ACCEPT
Summary: p53 regulates DNA-templated transcription as both an activator and repressor.
Reason: Core molecular function - transcription regulation is fundamental to p53's tumor suppressor activity.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:25417702
Functional interplay between MDM2, p63/p73 and mutant p53.
PMID:7587074
Targets for transcriptional activation by wild-type p53: endogenous retroviral LTR, immunoglobulin-like promoter, and an internal promoter of the mdm2 gene.
GO:0006915 apoptotic process
IEA
GO_REF:0000120 		ACCEPT
Summary: p53 is a master regulator of apoptosis, inducing cell death through multiple pathways.
Reason: Core function - apoptosis induction is one of p53's primary tumor suppressor mechanisms.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
induces growth arrest or apoptosis depending on the physiological circumstances
GO:0010212 response to ionizing radiation
IEA
GO_REF:0000117 		ACCEPT
Summary: p53 is strongly activated by ionizing radiation-induced DNA damage.
Reason: Core function - ionizing radiation is a classic p53-activating stress through ATM/ATR signaling.
Supporting Evidence:
TP53-deep-research
p53 serves as a central node in the DNA damage response network
GO:0012501 programmed cell death
IEA
GO_REF:0000043 		ACCEPT
Summary: p53 induces programmed cell death through apoptosis, and also regulates other cell death pathways.
Reason: Core function - p53 is a master regulator of programmed cell death.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
induces growth arrest or apoptosis
GO:0016605 PML body
IEA
GO_REF:0000044 		ACCEPT
Summary: p53 localizes to PML nuclear bodies, which is important for its post-translational modification and activation.
Reason: Important regulatory localization - p53 accumulates in PML bodies during stress responses where it undergoes acetylation.
Supporting Evidence:
TP53-deep-research
p53 localizes to nuclear bodies like PML bodies, especially during stress responses
PMID:22869143
BMK1 is involved in the regulation of p53 through disrupting the PML-MDM2 interaction.
PMID:12006491
Human SIR2 deacetylates p53 and antagonizes PML/p53-induced cellular senescence.
GO:0046872 metal ion binding
IEA
GO_REF:0000043 		ACCEPT
Summary: p53's DNA-binding domain contains a zinc coordination site essential for structural stability.
Reason: Core structural feature - the p53 DNA-binding domain contains a zinc atom coordinated by Cys176, His179, Cys238, and Cys242.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Contains zinc coordination site in DNA-binding domain
GO:0048511 rhythmic process
IEA
GO_REF:0000043 		KEEP AS NON CORE
Summary: p53 may influence circadian rhythms but this is not a core function.
Reason: Non-core function. p53 has connections to circadian biology but this is not its primary role.
GO:0051053 negative regulation of DNA metabolic process
IEA
GO_REF:0000117 		ACCEPT
Summary: p53 negatively regulates DNA replication as part of cell cycle arrest response.
Reason: Core function related to cell cycle control - p53 inhibits DNA replication in damaged cells.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Negatively regulates cell division
GO:0051262 protein tetramerization
IEA
GO_REF:0000002 		ACCEPT
Summary: p53 forms tetramers through its oligomerization domain (residues 325-356), essential for DNA binding.
Reason: Core structural feature - tetramerization is essential for high-affinity DNA binding and p53 function.
Supporting Evidence:
PDB:1C26
Crystal structure of p53 tetramerization domain shows dimer-of-dimers architecture
GO:0071456 cellular response to hypoxia
IEA
GO_REF:0000117 		ACCEPT
Summary: p53 mediates cellular responses to hypoxia, including cell cycle arrest and apoptosis induction.
Reason: Core stress response function - p53 responds to hypoxic stress and coordinates appropriate cellular responses.
Supporting Evidence:
GO:1990144
intrinsic apoptotic signaling pathway in response to hypoxia
PMID:20810912
Hypoxia downregulates p53 but induces apoptosis and enhances expression of BAD in cultures of human syncytiotrophoblasts.
GO:0097190 apoptotic signaling pathway
IEA
GO_REF:0000117 		ACCEPT
Summary: p53 is a master regulator of apoptotic signaling pathways.
Reason: Core function - p53 activates both intrinsic and extrinsic apoptotic signaling.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression
GO:2001242 regulation of intrinsic apoptotic signaling pathway
IEA
GO_REF:0000117 		ACCEPT
Summary: p53 both executes and regulates the intrinsic apoptotic signaling pathway.
Reason: Core function - p53 is a key regulator of intrinsic apoptotic signaling through multiple target genes.
Supporting Evidence:
TP53-deep-research
p53 triggers apoptosis through both transcription-dependent and transcription-independent mechanisms
GO:0005515 protein binding
IPI
PMID:10196247
Characterization of an E1A-CBP interaction defines a novel t... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:10196247
Characterization of an E1A-CBP interaction defines a novel transcriptional adapter motif (TRAM) in CBP/p300.
GO:0005515 protein binding
IPI
PMID:10226625
The yeast two-hybrid system reveals no interaction between p... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:10226625
The yeast two-hybrid system reveals no interaction between p73 alpha and SV40 large T-antigen.
GO:0005515 protein binding
IPI
PMID:10518217
A novel cofactor for p300 that regulates the p53 response. 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:10518217
A novel cofactor for p300 that regulates the p53 response.
GO:0005515 protein binding
IPI
PMID:10823891
The Huntington's disease protein interacts with p53 and CREB... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:10823891
The Huntington's disease protein interacts with p53 and CREB-binding protein and represses transcription.
GO:0005515 protein binding
IPI
PMID:11146555
Functional interaction between p53 and the interferon-induci... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:11146555
Functional interaction between p53 and the interferon-inducible nucleoprotein IFI 16.
GO:0005515 protein binding
IPI
PMID:11178989
MDM2 enhances the function of estrogen receptor alpha in hum... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:11178989
MDM2 enhances the function of estrogen receptor alpha in human breast cancer cells.
GO:0005515 protein binding
IPI
PMID:11359905
The corepressor mSin3a interacts with the proline-rich domai... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:11359905
The corepressor mSin3a interacts with the proline-rich domain of p53 and protects p53 from proteasome-mediated degradation.
GO:0005515 protein binding
IPI
PMID:11388671
A putative protein inhibitor of activated STAT (PIASy) inter... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:11388671
A putative protein inhibitor of activated STAT (PIASy) interacts with p53 and inhibits p53-mediated transactivation but not apoptosis.
GO:0005515 protein binding
IPI
PMID:11427532
p53 Modulates the exonuclease activity of Werner syndrome pr... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:11427532
2001 Jun 26. p53 Modulates the exonuclease activity of Werner syndrome protein.
GO:0005515 protein binding
IPI
PMID:11672523
hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase. 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:11672523
hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase.
GO:0005515 protein binding
IPI
PMID:11706030
Differential effect of ik3-1/cables on p53- and p73-induced ... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:11706030
Nov 12. Differential effect of ik3-1/cables on p53- and p73-induced cell death.
GO:0005515 protein binding
IPI
PMID:11877378
Structure of the 53BP1 BRCT region bound to p53 and its comp... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:11877378
Structure of the 53BP1 BRCT region bound to p53 and its comparison to the Brca1 BRCT structure.
GO:0005515 protein binding
IPI
PMID:12006491
Human SIR2 deacetylates p53 and antagonizes PML/p53-induced ... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:12006491
Human SIR2 deacetylates p53 and antagonizes PML/p53-induced cellular senescence.
GO:0005515 protein binding
IPI
PMID:12080348
Nucleophosmin regulates the stability and transcriptional ac... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:12080348
Nucleophosmin regulates the stability and transcriptional activity of p53.
GO:0005515 protein binding
IPI
PMID:12620801
Requirement of E6AP and the features of human papillomavirus... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:12620801
Requirement of E6AP and the features of human papillomavirus E6 necessary to support degradation of p53.
GO:0005515 protein binding
IPI
PMID:12667443
p53 has a direct apoptogenic role at the mitochondria. 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:12667443
p53 has a direct apoptogenic role at the mitochondria.
GO:0005515 protein binding
IPI
PMID:12692135
P63 alpha mutations lead to aberrant splicing of keratinocyt... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:12692135
2003 Apr 10. P63 alpha mutations lead to aberrant splicing of keratinocyte growth factor receptor in the Hay-Wells syndrome.
GO:0005515 protein binding
IPI
PMID:12702766
Characterization of cells and gene-targeted mice deficient f... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:12702766
Characterization of cells and gene-targeted mice deficient for the p53-binding kinase homeodomain-interacting protein kinase 1 (HIPK1).
GO:0005515 protein binding
IPI
PMID:12915590
Cellular stress and DNA damage invoke temporally distinct Md... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
GO:0005515 protein binding
IPI
PMID:14557665
Adenovirus E1B 55-kilodalton oncoprotein binds to Daxx and e... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:14557665
Adenovirus E1B 55-kilodalton oncoprotein binds to Daxx and eliminates enhancement of p53-dependent transcription by Daxx.
GO:0005515 protein binding
IPI
PMID:14627987
PARP-1 binds E2F-1 independently of its DNA binding and cata... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:14627987
PARP-1 binds E2F-1 independently of its DNA binding and catalytic domains, and acts as a novel coactivator of E2F-1-mediated transcription during re-entry of quiescent cells into S phase.
GO:0005515 protein binding
IPI
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localiz... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
GO:0005515 protein binding
IPI
PMID:14759370
Structural mechanism of the bromodomain of the coactivator C... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:14759370
Structural mechanism of the bromodomain of the coactivator CBP in p53 transcriptional activation.
GO:0005515 protein binding
IPI
PMID:14963330
Direct activation of Bax by p53 mediates mitochondrial membr... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:14963330
Direct activation of Bax by p53 mediates mitochondrial membrane permeabilization and apoptosis.
GO:0005515 protein binding
IPI
PMID:14985081
Hepatitis C virus core protein interacts with p53-binding pr... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:14985081
Hepatitis C virus core protein interacts with p53-binding protein, 53BP2/Bbp/ASPP2, and inhibits p53-mediated apoptosis.
GO:0005515 protein binding
IPI
PMID:15044383
Aprataxin, a novel protein that protects against genotoxic s... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:15044383
Mar 25. Aprataxin, a novel protein that protects against genotoxic stress.
GO:0005515 protein binding
IPI
PMID:15068796
C. elegans SGK-1 is the critical component in the Akt/PKB ki... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:15068796
C. elegans SGK-1 is the critical component in the Akt/PKB kinase complex to control stress response and life span.
GO:0005515 protein binding
IPI
PMID:15133049
NMR structure of the N-terminal domain of SUMO ligase PIAS1 ... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:15133049
2004 May 8. NMR structure of the N-terminal domain of SUMO ligase PIAS1 and its interaction with tumor suppressor p53 and A/T-rich DNA oligomers.
GO:0005515 protein binding
IPI
PMID:15144954
Nucleolar protein NPM interacts with HDM2 and protects tumor... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:15144954
Nucleolar protein NPM interacts with HDM2 and protects tumor suppressor protein p53 from HDM2-mediated degradation.
GO:0005515 protein binding
IPI
PMID:15205477
Calorie restriction promotes mammalian cell survival by indu... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:15205477
Jun 17. Calorie restriction promotes mammalian cell survival by inducing the SIRT1 deacetylase.
GO:0005515 protein binding
IPI
PMID:15310821
Identification of ASK and clock-associated proteins as molec... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:15310821
Identification of ASK and clock-associated proteins as molecular partners of LKP2 (LOV kelch protein 2) in Arabidopsis.
GO:0005515 protein binding
IPI
PMID:15364927
Negative regulation of p53 functions by Daxx and the involve... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:15364927
2004 Sep 10. Negative regulation of p53 functions by Daxx and the involvement of MDM2.
GO:0005515 protein binding
IPI
PMID:15525938
Regulation of p53 activity through lysine methylation. 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:15525938
Regulation of p53 activity through lysine methylation.
GO:0005515 protein binding
IPI
PMID:15542844
p53 Stabilization and accumulation induced by human vaccinia... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:15542844
p53 Stabilization and accumulation induced by human vaccinia-related kinase 1.
GO:0005515 protein binding
IPI
PMID:15580310
17beta-Estradiol upregulates and activates WOX1/WWOXv1 and W... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:15580310
17beta-Estradiol upregulates and activates WOX1/WWOXv1 and WOX2/WWOXv2 in vitro: potential role in cancerous progression of breast and prostate to a premetastatic state in vivo.
GO:0005515 protein binding
IPI
PMID:15604276
Adenoviral E1A targets Mdm4 to stabilize tumor suppressor p5... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:15604276
Adenoviral E1A targets Mdm4 to stabilize tumor suppressor p53.
GO:0005515 protein binding
IPI
PMID:15660129
The DEAD box protein p68: a novel transcriptional coactivato... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:15660129
The DEAD box protein p68: a novel transcriptional coactivator of the p53 tumour suppressor.
GO:0005515 protein binding
IPI
PMID:15735006
p53 modulates RPA-dependent and RPA-independent WRN helicase... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:15735006
p53 modulates RPA-dependent and RPA-independent WRN helicase activity.
GO:0005515 protein binding
IPI
PMID:15782130
BARD1 induces apoptosis by catalysing phosphorylation of p53... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:15782130
BARD1 induces apoptosis by catalysing phosphorylation of p53 by DNA-damage response kinase.
GO:0005515 protein binding
IPI
PMID:15855171
Direct interaction of the N-terminal domain of focal adhesio... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:15855171
2005 Apr 25. Direct interaction of the N-terminal domain of focal adhesion kinase with the N-terminal transactivation domain of p53.
GO:0005515 protein binding
IPI
PMID:15916963
Loss of HAUSP-mediated deubiquitination contributes to DNA d... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:15916963
Loss of HAUSP-mediated deubiquitination contributes to DNA damage-induced destabilization of Hdmx and Hdm2.
GO:0005515 protein binding
IPI
PMID:15960975
Physical association and coordinate function of the H3 K4 me... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:15960975
Physical association and coordinate function of the H3 K4 methyltransferase MLL1 and the H4 K16 acetyltransferase MOF.
GO:0005515 protein binding
IPI
PMID:15989956
ARF-BP1/Mule is a critical mediator of the ARF tumor suppres... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:15989956
ARF-BP1/Mule is a critical mediator of the ARF tumor suppressor.
GO:0005515 protein binding
IPI
PMID:16003391
AtBAG6, a novel calmodulin-binding protein, induces programm... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:16003391
AtBAG6, a novel calmodulin-binding protein, induces programmed cell death in yeast and plants.
GO:0005515 protein binding
IPI
PMID:16151013
PUMA couples the nuclear and cytoplasmic proapoptotic functi... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:16151013
PUMA couples the nuclear and cytoplasmic proapoptotic function of p53.
GO:0005515 protein binding
IPI
PMID:16169070
A human protein-protein interaction network: a resource for ... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:16169070
A human protein-protein interaction network: a resource for annotating the proteome.
GO:0005515 protein binding
IPI
PMID:16189514
Towards a proteome-scale map of the human protein-protein in... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:16189514
Towards a proteome-scale map of the human protein-protein interaction network.
GO:0005515 protein binding
IPI
PMID:16227626
Physical interaction and mutual transrepression between CCAA... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:16227626
Oct 14. Physical interaction and mutual transrepression between CCAAT/enhancer-binding protein beta and the p53 tumor suppressor.
GO:0005515 protein binding
IPI
PMID:16319068
Camptothecin induces nuclear export of prohibitin preferenti... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:16319068
Nov 30. Camptothecin induces nuclear export of prohibitin preferentially in transformed cells through a CRM-1-dependent mechanism.
GO:0005515 protein binding
IPI
PMID:16322561
NIR is a novel INHAT repressor that modulates the transcript... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:16322561
NIR is a novel INHAT repressor that modulates the transcriptional activity of p53.
GO:0005515 protein binding
IPI
PMID:16376338
Protein kinase A phosphorylates and regulates dimerization o... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:16376338
2005 Dec 19. Protein kinase A phosphorylates and regulates dimerization of 14-3-3 epsilon.
GO:0005515 protein binding
IPI
PMID:16376884
Characterisation of the interface between nucleophosmin (NPM... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:16376884
2005 Dec 19. Characterisation of the interface between nucleophosmin (NPM) and p53: potential role in p53 stabilisation.
GO:0005515 protein binding
IPI
PMID:16377624
Protein kinase C delta regulates Ser46 phosphorylation of p5... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:16377624
Dec 23. Protein kinase C delta regulates Ser46 phosphorylation of p53 tumor suppressor in the apoptotic response to DNA damage.
GO:0005515 protein binding
IPI
PMID:16402859
Structural basis of competitive recognition of p53 and MDM2 ... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:16402859
Structural basis of competitive recognition of p53 and MDM2 by HAUSP/USP7: implications for the regulation of the p53-MDM2 pathway.
GO:0005515 protein binding
IPI
PMID:16415881
Structural basis for the methylation site specificity of SET... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:16415881
Jan 15. Structural basis for the methylation site specificity of SET7/9.
GO:0005515 protein binding
IPI
PMID:16432196
The central region of HDM2 provides a second binding site fo... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:16432196
The central region of HDM2 provides a second binding site for p53.
GO:0005515 protein binding
IPI
PMID:16442532
Interaction of metallothionein with tumor suppressor p53 pro... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:16442532
2006 Jan 20. Interaction of metallothionein with tumor suppressor p53 protein.
GO:0005515 protein binding
IPI
PMID:16474402
Molecular recognition of p53 and MDM2 by USP7/HAUSP. 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:16474402
Feb 12. Molecular recognition of p53 and MDM2 by USP7/HAUSP.
GO:0005515 protein binding
IPI
PMID:16493710
Surface plasmon resonance imaging protein arrays for analysi... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:16493710
Surface plasmon resonance imaging protein arrays for analysis of triple protein interactions of HPV, E6, E6AP, and p53.
GO:0005515 protein binding
IPI
PMID:16511572
14-3-3gamma binds to MDMX that is phosphorylated by UV-activ... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:16511572
Mar 2. 14-3-3gamma binds to MDMX that is phosphorylated by UV-activated Chk1, resulting in p53 activation.
GO:0005515 protein binding
IPI
PMID:16601686
Tip60 and p400 are both required for UV-induced apoptosis bu... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:16601686
Tip60 and p400 are both required for UV-induced apoptosis but play antagonistic roles in cell cycle progression.
GO:0005515 protein binding
IPI
PMID:16611888
Targeting of p300/CREB binding protein coactivators by simia... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:16611888
Targeting of p300/CREB binding protein coactivators by simian virus 40 is mediated through p53.
GO:0005515 protein binding
IPI
PMID:16616141
Evidence that low doses of Taxol enhance the functional tran... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:16616141
Epub 2006 Mar 29. Evidence that low doses of Taxol enhance the functional transactivatory properties of p53 on p21 waf promoter in MCF-7 breast cancer cells.
GO:0005515 protein binding
IPI
PMID:16690937
NS3 protein of Hepatitis C virus associates with the tumour ... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:16690937
NS3 protein of Hepatitis C virus associates with the tumour suppressor p53 and inhibits its function in an NS3 sequence-dependent manner.
GO:0005515 protein binding
IPI
PMID:16713569
A protein-protein interaction network for human inherited at... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:16713569
A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration.
GO:0005515 protein binding
IPI
PMID:16732283
Lysine activation and functional analysis of E2-mediated con... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:16732283
May 28. Lysine activation and functional analysis of E2-mediated conjugation in the SUMO pathway.
GO:0005515 protein binding
IPI
PMID:16753148
Polo-like kinase 1 regulates mitotic arrest after UV irradia... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:16753148
Epub 2006 May 30. Polo-like kinase 1 regulates mitotic arrest after UV irradiation through dephosphorylation of p53 and inducing p53 degradation.
GO:0005515 protein binding
IPI
PMID:16793543
Structure of the Tfb1/p53 complex: Insights into the interac... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:16793543
Structure of the Tfb1/p53 complex: Insights into the interaction between the p62/Tfb1 subunit of TFIIH and the activation domain of p53.
GO:0005515 protein binding
IPI
PMID:16845383
Critical role for Daxx in regulating Mdm2. 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:16845383
Critical role for Daxx in regulating Mdm2.
GO:0005515 protein binding
IPI
PMID:16847267
MAGE-A tumor antigens target p53 transactivation function th... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:16847267
MAGE-A tumor antigens target p53 transactivation function through histone deacetylase recruitment and confer resistance to chemotherapeutic agents.
GO:0005515 protein binding
IPI
PMID:16951253
Crystal structure of SV40 large T-antigen bound to p53: inte... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:16951253
Crystal structure of SV40 large T-antigen bound to p53: interplay between a viral oncoprotein and a cellular tumor suppressor.
GO:0005515 protein binding
IPI
PMID:16959611
Gain of function of mutant p53: the mutant p53/NF-Y protein ... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:16959611
Gain of function of mutant p53: the mutant p53/NF-Y protein complex reveals an aberrant transcriptional mechanism of cell cycle regulation.
GO:0005515 protein binding
IPI
PMID:17080083
Inactivation of the p53 pathway in retinoblastoma. 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:17080083
Inactivation of the p53 pathway in retinoblastoma.
GO:0005515 protein binding
IPI
PMID:17098746
FBXO11 promotes the Neddylation of p53 and inhibits its tran... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:17098746
2006 Nov 9. FBXO11 promotes the Neddylation of p53 and inhibits its transcriptional activity.
GO:0005515 protein binding
IPI
PMID:17108971
Repression of p53 activity by Smyd2-mediated methylation. 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:17108971
Repression of p53 activity by Smyd2-mediated methylation.
GO:0005515 protein binding
IPI
PMID:17139261
p53 mediates the negative regulation of MDM2 by orphan recep... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:17139261
Nov 30. p53 mediates the negative regulation of MDM2 by orphan receptor TR3.
GO:0005515 protein binding
IPI
PMID:17159902
An essential function of the extreme C-terminus of MDM2 can ... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:17159902
An essential function of the extreme C-terminus of MDM2 can be provided by MDMX.
GO:0005515 protein binding
IPI
PMID:17170702
Cytoplasmic destruction of p53 by the endoplasmic reticulum-... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:17170702
Cytoplasmic destruction of p53 by the endoplasmic reticulum-resident ubiquitin ligase 'Synoviolin'.
GO:0005515 protein binding
IPI
PMID:17184779
Identification of differential proteins in nasopharyngeal ca... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:17184779
2006 Dec 13. Identification of differential proteins in nasopharyngeal carcinoma cells with p53 silence by proteome analysis.
GO:0005515 protein binding
IPI
PMID:17245430
A specific PP2A regulatory subunit, B56gamma, mediates DNA d... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:17245430
A specific PP2A regulatory subunit, B56gamma, mediates DNA damage-induced dephosphorylation of p53 at Thr55.
GO:0005515 protein binding
IPI
PMID:17254968
PRAK is essential for ras-induced senescence and tumor suppr... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:17254968
PRAK is essential for ras-induced senescence and tumor suppression.
GO:0005515 protein binding
IPI
PMID:17268548
Monoubiquitylation promotes mitochondrial p53 translocation. 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:17268548
Monoubiquitylation promotes mitochondrial p53 translocation.
GO:0005515 protein binding
IPI
PMID:17274640
A limited screen for protein interactions reveals new roles ... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:17274640
A limited screen for protein interactions reveals new roles for protein phosphatase 1 in cell cycle control and apoptosis.
GO:0005515 protein binding
IPI
PMID:17290220
The deubiquitinating enzyme USP2a regulates the p53 pathway ... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:17290220
The deubiquitinating enzyme USP2a regulates the p53 pathway by targeting Mdm2.
GO:0005515 protein binding
IPI
PMID:17298945
The conserved CPH domains of Cul7 and PARC are protein-prote... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:17298945
2007 Feb 12. The conserved CPH domains of Cul7 and PARC are protein-protein interaction modules that bind the tetramerization domain of p53.
GO:0005515 protein binding
IPI
PMID:17310983
Ribosomal protein S7 as a novel modulator of p53-MDM2 intera... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:17310983
Feb 19. Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function.
GO:0005515 protein binding
IPI
PMID:17347673
Stra13 is induced by genotoxic stress and regulates ionizing... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:17347673
Stra13 is induced by genotoxic stress and regulates ionizing-radiation-induced apoptosis.
GO:0005515 protein binding
IPI
PMID:17438265
Four domains of p300 each bind tightly to a sequence spannin... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:17438265
Four domains of p300 each bind tightly to a sequence spanning both transactivation subdomains of p53.
GO:0005515 protein binding
IPI
PMID:17470788
PACT is a negative regulator of p53 and essential for cell g... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:17470788
PACT is a negative regulator of p53 and essential for cell growth and embryonic development.
GO:0005515 protein binding
IPI
PMID:17482142
Attenuation of DNA damage checkpoint by PBK, a novel mitotic... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:17482142
Attenuation of DNA damage checkpoint by PBK, a novel mitotic kinase, involves protein-protein interaction with tumor suppressor p53.
GO:0005515 protein binding
IPI
PMID:17568776
Phosphorylation of Pirh2 by calmodulin-dependent kinase II i... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:17568776
Jun 14. Phosphorylation of Pirh2 by calmodulin-dependent kinase II impairs its ability to ubiquitinate p53.
GO:0005515 protein binding
IPI
PMID:17612295
Yeast split-ubiquitin-based cytosolic screening system to de... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:17612295
Yeast split-ubiquitin-based cytosolic screening system to detect interactions between transcriptionally active proteins.
GO:0005515 protein binding
IPI
PMID:17662718
Protein-protein interactions among human lens acidic and bas... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:17662718
Epub 2007 Jul 23. Protein-protein interactions among human lens acidic and basic beta-crystallins.
GO:0005515 protein binding
IPI
PMID:17719542
hCAS/CSE1L associates with chromatin and regulates expressio... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:17719542
hCAS/CSE1L associates with chromatin and regulates expression of select p53 target genes.
GO:0005515 protein binding
IPI
PMID:17805299
p53 is regulated by the lysine demethylase LSD1. 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:17805299
p53 is regulated by the lysine demethylase LSD1.
GO:0005515 protein binding
IPI
PMID:17875722
Efficient p53 activation and apoptosis by simultaneous disru... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:17875722
Efficient p53 activation and apoptosis by simultaneous disruption of binding to MDM2 and MDMX.
GO:0005515 protein binding
IPI
PMID:17906639
The prolyl isomerase Pin1 orchestrates p53 acetylation and d... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:17906639
Sep 30. The prolyl isomerase Pin1 orchestrates p53 acetylation and dissociation from the apoptosis inhibitor iASPP.
GO:0005515 protein binding
IPI
PMID:17964266
Active regulator of SIRT1 cooperates with SIRT1 and facilita... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:17964266
Active regulator of SIRT1 cooperates with SIRT1 and facilitates suppression of p53 activity.
GO:0005515 protein binding
IPI
PMID:18087040
Regulation of p53 tetramerization and nuclear export by ARC. 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:18087040
Regulation of p53 tetramerization and nuclear export by ARC.
GO:0005515 protein binding
IPI
PMID:18172499
NUMB controls p53 tumour suppressor activity. 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:18172499
NUMB controls p53 tumour suppressor activity.
GO:0005515 protein binding
IPI
PMID:18230339
Activation of p53-dependent responses in tumor cells treated... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:18230339
Activation of p53-dependent responses in tumor cells treated with a PARC-interacting peptide.
GO:0005515 protein binding
IPI
PMID:18235501
DBC1 is a negative regulator of SIRT1. 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:18235501
DBC1 is a negative regulator of SIRT1.
GO:0005515 protein binding
IPI
PMID:18235502
Negative regulation of the deacetylase SIRT1 by DBC1. 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:18235502
Negative regulation of the deacetylase SIRT1 by DBC1.
GO:0005515 protein binding
IPI
PMID:18275817
Biochemical and structural studies of ASPP proteins reveal d... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:18275817
Biochemical and structural studies of ASPP proteins reveal differential binding to p53, p63, and p73.
GO:0005515 protein binding
IPI
PMID:18309296
Proteasome activator PA28 gamma regulates p53 by enhancing i... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:18309296
Proteasome activator PA28 gamma regulates p53 by enhancing its MDM2-mediated degradation.
GO:0005515 protein binding
IPI
PMID:18316739
Temporal activation of p53 by a specific MDM2 inhibitor is s... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:18316739
Temporal activation of p53 by a specific MDM2 inhibitor is selectively toxic to tumors and leads to complete tumor growth inhibition.
GO:0005515 protein binding
IPI
PMID:18354501
Structural insight into the TFIIE-TFIIH interaction: TFIIE a... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:18354501
Structural insight into the TFIIE-TFIIH interaction: TFIIE and p53 share the binding region on TFIIH.
GO:0005515 protein binding
IPI
PMID:18388957
NAD(P)H quinone oxidoreductase 1 inhibits the proteasomal de... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:18388957
NAD(P)H quinone oxidoreductase 1 inhibits the proteasomal degradation of the tumour suppressor p33(ING1b).
GO:0005515 protein binding
IPI
PMID:18391200
Structure of tumor suppressor p53 and its intrinsically diso... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:18391200
Structure of tumor suppressor p53 and its intrinsically disordered N-terminal transactivation domain.
GO:0005515 protein binding
IPI
PMID:18485870
Acetylation is indispensable for p53 activation. 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:18485870
Acetylation is indispensable for p53 activation.
GO:0005515 protein binding
IPI
PMID:18504427
Twist and p53 reciprocally regulate target genes via direct ... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:18504427
Twist and p53 reciprocally regulate target genes via direct interaction.
GO:0005515 protein binding
IPI
PMID:18510931
Ectodermal factor restricts mesoderm differentiation by inhi... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:18510931
Ectodermal factor restricts mesoderm differentiation by inhibiting p53.
GO:0005515 protein binding
IPI
PMID:18566590
The tumour suppressor RASSF1A promotes MDM2 self-ubiquitinat... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:18566590
The tumour suppressor RASSF1A promotes MDM2 self-ubiquitination by disrupting the MDM2-DAXX-HAUSP complex.
GO:0005515 protein binding
IPI
PMID:18624398
Protein interaction data set highlighted with human Ras-MAPK... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:18624398
Protein interaction data set highlighted with human Ras-MAPK/PI3K signaling pathways.
GO:0005515 protein binding
IPI
PMID:18656471
Microtubule-associated protein 1B light chain (MAP1B-LC1) ne... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:18656471
Epub 2008 Jul 24. Microtubule-associated protein 1B light chain (MAP1B-LC1) negatively regulates the activity of tumor suppressor p53 in neuroblastoma cells.
GO:0005515 protein binding
IPI
PMID:18695251
AIMP2/p38, the scaffold for the multi-tRNA synthetase comple... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:18695251
AIMP2/p38, the scaffold for the multi-tRNA synthetase complex, responds to genotoxic stresses via p53.
GO:0005515 protein binding
IPI
PMID:18812399
14-3-3 activation of DNA binding of p53 by enhancing its ass... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:18812399
Sep 23. 14-3-3 activation of DNA binding of p53 by enhancing its association into tetramers.
GO:0005515 protein binding
IPI
PMID:18952844
Inhibition of Thr-55 phosphorylation restores p53 nuclear lo... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:18952844
Inhibition of Thr-55 phosphorylation restores p53 nuclear localization and sensitizes cancer cells to DNA damage.
GO:0005515 protein binding
IPI
PMID:18977328
Inactivation of the CYLD deubiquitinase by HPV E6 mediates h... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:18977328
Inactivation of the CYLD deubiquitinase by HPV E6 mediates hypoxia-induced NF-kappaB activation.
GO:0005515 protein binding
IPI
PMID:19008854
Interferon-inducible protein, P56, inhibits HPV DNA replicat... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:19008854
Interferon-inducible protein, P56, inhibits HPV DNA replication by binding to the viral protein E1.
GO:0005515 protein binding
IPI
PMID:19043414
Molecular basis of Pirh2-mediated p53 ubiquitylation. 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:19043414
2008 Nov 30. Molecular basis of Pirh2-mediated p53 ubiquitylation.
GO:0005515 protein binding
IPI
PMID:19098711
RYBP stabilizes p53 by modulating MDM2. 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:19098711
RYBP stabilizes p53 by modulating MDM2.
GO:0005515 protein binding
IPI
PMID:19166840
FKBP25, a novel regulator of the p53 pathway, induces the de... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:19166840
2009 Jan 21. FKBP25, a novel regulator of the p53 pathway, induces the degradation of MDM2 and activation of p53.
GO:0005515 protein binding
IPI
PMID:19196987
Phosphorylation of p53 by IkappaB kinase 2 promotes its degr... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:19196987
Phosphorylation of p53 by IkappaB kinase 2 promotes its degradation by beta-TrCP.
GO:0005515 protein binding
IPI
PMID:19217391
Structural basis for p300 Taz2-p53 TAD1 binding and modulati... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:19217391
Structural basis for p300 Taz2-p53 TAD1 binding and modulation by phosphorylation.
GO:0005515 protein binding
IPI
PMID:19234109
Induction of SOX4 by DNA damage is critical for p53 stabiliz... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:19234109
Induction of SOX4 by DNA damage is critical for p53 stabilization and function.
GO:0005515 protein binding
IPI
PMID:19255450
Structural basis for high-affinity peptide inhibition of p53... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:19255450
Structural basis for high-affinity peptide inhibition of p53 interactions with MDM2 and MDMX.
GO:0005515 protein binding
IPI
PMID:19339993
Crosstalk between sumoylation and acetylation regulates p53-... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:19339993
Crosstalk between sumoylation and acetylation regulates p53-dependent chromatin transcription and DNA binding.
GO:0005515 protein binding
IPI
PMID:19345189
A Mutant-p53/Smad complex opposes p63 to empower TGFbeta-ind... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:19345189
A Mutant-p53/Smad complex opposes p63 to empower TGFbeta-induced metastasis.
GO:0005515 protein binding
IPI
PMID:19411066
Regulation of XIAP translation and induction by MDM2 followi... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:19411066
Regulation of XIAP translation and induction by MDM2 following irradiation.
GO:0005515 protein binding
IPI
PMID:19433796
A tumor suppressive coactivator complex of p53 containing AS... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:19433796
A tumor suppressive coactivator complex of p53 containing ASC-2 and histone H3-lysine-4 methyltransferase MLL3 or its paralogue MLL4.
GO:0005515 protein binding
IPI
PMID:19508870
TOE1 interacts with p53 to modulate its transactivation pote... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:19508870
Epub 2009 Jun 7. TOE1 interacts with p53 to modulate its transactivation potential.
GO:0005515 protein binding
IPI
PMID:19521340
MDM4 (MDMX) localizes at the mitochondria and facilitates th... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:19521340
MDM4 (MDMX) localizes at the mitochondria and facilitates the p53-mediated intrinsic-apoptotic pathway.
GO:0005515 protein binding
IPI
PMID:19536131
Differential regulation of p53 and p21 by MKRN1 E3 ligase co... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:19536131
Differential regulation of p53 and p21 by MKRN1 E3 ligase controls cell cycle arrest and apoptosis.
GO:0005515 protein binding
IPI
PMID:19556538
Trim24 targets endogenous p53 for degradation. 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:19556538
Trim24 targets endogenous p53 for degradation.
GO:0005515 protein binding
IPI
PMID:19619542
Mdmx enhances p53 ubiquitination by altering the substrate p... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:19619542
2009 Jul 18. Mdmx enhances p53 ubiquitination by altering the substrate preference of the Mdm2 ubiquitin ligase.
GO:0005515 protein binding
IPI
PMID:19626115
Modulation of microRNA processing by p53. 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:19626115
Modulation of microRNA processing by p53.
GO:0005515 protein binding
IPI
PMID:19651603
Structural basis for subversion of cellular control mechanis... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:19651603
Structural basis for subversion of cellular control mechanisms by the adenoviral E1A oncoprotein.
GO:0005515 protein binding
IPI
PMID:19656744
Ribosomal protein S3: A multi-functional protein that intera... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:19656744
Ribosomal protein S3: A multi-functional protein that interacts with both p53 and MDM2 through its KH domain.
GO:0005515 protein binding
IPI
PMID:19680552
CK2 is the regulator of SIRT1 substrate-binding affinity, de... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:19680552
CK2 is the regulator of SIRT1 substrate-binding affinity, deacetylase activity and cellular response to DNA-damage.
GO:0005515 protein binding
IPI
PMID:19684601
A molecular basis for phosphorylation-dependent SUMO conjuga... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:19684601
Aug 16. A molecular basis for phosphorylation-dependent SUMO conjugation by the E2 UBC9.
GO:0005515 protein binding
IPI
PMID:19740107
Epidermal growth factor receptor ligands as new extracellula... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:19740107
2009 Sep 9. Epidermal growth factor receptor ligands as new extracellular targets for the metastasis-promoting S100A4 protein.
GO:0005515 protein binding
IPI
PMID:19798103
Efficient protection and isolation of ubiquitylated proteins... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:19798103
Efficient protection and isolation of ubiquitylated proteins using tandem ubiquitin-binding entities.
GO:0005515 protein binding
IPI
PMID:19805293
CBP and p300 are cytoplasmic E4 polyubiquitin ligases for p5... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:19805293
CBP and p300 are cytoplasmic E4 polyubiquitin ligases for p53.
GO:0005515 protein binding
IPI
PMID:19833129
Polo-like kinase-1 phosphorylates MDM2 at Ser260 and stimula... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:19833129
Epub 2009 Oct 13. Polo-like kinase-1 phosphorylates MDM2 at Ser260 and stimulates MDM2-mediated p53 turnover.
GO:0005515 protein binding
IPI
PMID:19857493
Role of p53/FAK association and p53Ser46 phosphorylation in ... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:19857493
Epub 2009 Oct 24. Role of p53/FAK association and p53Ser46 phosphorylation in staurosporine-mediated apoptosis: wild type versus mutant p53-R175H.
GO:0005515 protein binding
IPI
PMID:19933256
Mechanistic differences in the transcriptional activation of... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:19933256
Nov 20. Mechanistic differences in the transcriptional activation of p53 by 14-3-3 isoforms.
GO:0005515 protein binding
IPI
PMID:20075864
Coordinated regulation of p53 apoptotic targets BAX and PUMA... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:20075864
Coordinated regulation of p53 apoptotic targets BAX and PUMA by SMAR1 through an identical MAR element.
GO:0005515 protein binding
IPI
PMID:20134482
Skp2B attenuates p53 function by inhibiting prohibitin. 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:20134482
Skp2B attenuates p53 function by inhibiting prohibitin.
GO:0005515 protein binding
IPI
PMID:20153329
Reconstitution of the mitochondrial Hsp70 (mortalin)-p53 int... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:20153329
2010 Feb 12. Reconstitution of the mitochondrial Hsp70 (mortalin)-p53 interaction using purified proteins--identification of additional interacting regions.
GO:0005515 protein binding
IPI
PMID:20159018
Identification and characterization of the novel protein CCD... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:20159018
Epub 2010 Feb 14. Identification and characterization of the novel protein CCDC106 that interacts with p53 and promotes its degradation.
GO:0005515 protein binding
IPI
PMID:20167603
DYRK1A and DYRK3 promote cell survival through phosphorylati... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:20167603
2010 Feb 18. DYRK1A and DYRK3 promote cell survival through phosphorylation and activation of SIRT1.
GO:0005515 protein binding
IPI
PMID:20173098
RFWD3-Mdm2 ubiquitin ligase complex positively regulates p53... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:20173098
RFWD3-Mdm2 ubiquitin ligase complex positively regulates p53 stability in response to DNA damage.
GO:0005515 protein binding
IPI
PMID:20206173
Structure of the p53 C-terminus bound to 14-3-3: implication... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:20206173
2010 Mar 3. Structure of the p53 C-terminus bound to 14-3-3: implications for stabilization of the p53 tetramer.
GO:0005515 protein binding
IPI
PMID:20227041
Modulation of the vitamin D3 response by cancer-associated m... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:20227041
Modulation of the vitamin D3 response by cancer-associated mutant p53.
GO:0005515 protein binding
IPI
PMID:20228809
BRD7 is a candidate tumour suppressor gene required for p53 ... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:20228809
BRD7 is a candidate tumour suppressor gene required for p53 function.
GO:0005515 protein binding
IPI
PMID:20385133
p53 inhibits tumor cell invasion via the degradation of snai... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:20385133
2010 Apr 10. p53 inhibits tumor cell invasion via the degradation of snail protein in hepatocellular carcinoma.
GO:0005515 protein binding
IPI
PMID:20421506
Mapping the physical and functional interactions between the... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:20421506
Mapping the physical and functional interactions between the tumor suppressors p53 and BRCA2.
GO:0005515 protein binding
IPI
PMID:20452352
A novel hPirh2 splicing variant without ubiquitin protein li... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:20452352
2010 May 7. A novel hPirh2 splicing variant without ubiquitin protein ligase activity interacts with p53 and is down-regulated in hepatocellular carcinoma.
GO:0005515 protein binding
IPI
PMID:20515689
MDM4 binds ligands via a mechanism in which disordered regio... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:20515689
Epub 2010 May 31. MDM4 binds ligands via a mechanism in which disordered regions become structured.
GO:0005515 protein binding
IPI
PMID:20534433
Synaptonemal complex formation and meiotic checkpoint signal... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:20534433
Synaptonemal complex formation and meiotic checkpoint signaling are linked to the lateral element protein Red1.
GO:0005515 protein binding
IPI
PMID:20562916
Dual-specificity phosphatase 26 is a novel p53 phosphatase a... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:20562916
Dual-specificity phosphatase 26 is a novel p53 phosphatase and inhibits p53 tumor suppressor functions in human neuroblastoma.
GO:0005515 protein binding
IPI
PMID:20591429
S100 proteins interact with the N-terminal domain of MDM2. 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:20591429
Epub 2010 Jun 19. S100 proteins interact with the N-terminal domain of MDM2.
GO:0005515 protein binding
IPI
PMID:20622899
PBK/TOPK interacts with the DBD domain of tumor suppressor p... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:20622899
PBK/TOPK interacts with the DBD domain of tumor suppressor p53 and modulates expression of transcriptional targets including p21.
GO:0005515 protein binding
IPI
PMID:20660729
Polybromo-associated BRG1-associated factor components BRD7 ... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:20660729
Polybromo-associated BRG1-associated factor components BRD7 and BAF180 are critical regulators of p53 required for induction of replicative senescence.
GO:0005515 protein binding
IPI
PMID:20705607
Turning the RING domain protein MdmX into an active ubiquiti... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:20705607
Epub 2010 Aug 12. Turning the RING domain protein MdmX into an active ubiquitin-protein ligase.
GO:0005515 protein binding
IPI
PMID:20708156
Phosphorylation by casein kinase I promotes the turnover of ... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:20708156
Phosphorylation by casein kinase I promotes the turnover of the Mdm2 oncoprotein via the SCF(beta-TRCP) ubiquitin ligase.
GO:0005515 protein binding
IPI
PMID:20713054
ARF-dependent regulation of ATM and p53 associated KZNF (Apa... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:20713054
Epub 2010 Aug 14. ARF-dependent regulation of ATM and p53 associated KZNF (Apak) protein activity in response to oncogenic stress.
GO:0005515 protein binding
IPI
PMID:20864041
MAGE-RING protein complexes comprise a family of E3 ubiquiti... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:20864041
MAGE-RING protein complexes comprise a family of E3 ubiquitin ligases.
GO:0005515 protein binding
IPI
PMID:21057547
AXIN is an essential co-activator for the promyelocytic leuk... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:21057547
Nov 8. AXIN is an essential co-activator for the promyelocytic leukemia protein in p53 activation.
GO:0005515 protein binding
IPI
PMID:21078964
p53-mediated apoptosis requires inositol hexakisphosphate ki... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:21078964
p53-mediated apoptosis requires inositol hexakisphosphate kinase-2.
GO:0005515 protein binding
IPI
PMID:21081126
Anti-apoptotic protein TCTP controls the stability of the tu... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:21081126
2010 Nov 17. Anti-apoptotic protein TCTP controls the stability of the tumor suppressor p53.
GO:0005515 protein binding
IPI
PMID:21130767
SAFB1 interacts with and suppresses the transcriptional acti... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:21130767
2010 Dec 3. SAFB1 interacts with and suppresses the transcriptional activity of p53.
GO:0005515 protein binding
IPI
PMID:21132010
GNL3L depletion destabilizes MDM2 and induces p53-dependent ... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:21132010
GNL3L depletion destabilizes MDM2 and induces p53-dependent G2/M arrest.
GO:0005515 protein binding
IPI
PMID:21170034
TSPYL5 suppresses p53 levels and function by physical intera... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:21170034
TSPYL5 suppresses p53 levels and function by physical interaction with USP7.
GO:0005515 protein binding
IPI
PMID:21170087
Ribosomal protein S27-like and S27 interplay with p53-MDM2 a... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:21170087
Dec 20. Ribosomal protein S27-like and S27 interplay with p53-MDM2 axis as a target, a substrate and a regulator.
GO:0005515 protein binding
IPI
PMID:21245319
Methyltransferase Set7/9 regulates p53 activity by interacti... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:21245319
Methyltransferase Set7/9 regulates p53 activity by interacting with Sirtuin 1 (SIRT1).
GO:0005515 protein binding
IPI
PMID:21317932
A new role of NUAK1: directly phosphorylating p53 and regula... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:21317932
A new role of NUAK1: directly phosphorylating p53 and regulating cell proliferation.
GO:0005515 protein binding
IPI
PMID:21390126
Inactivating mutations of acetyltransferase genes in B-cell ... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:21390126
Inactivating mutations of acetyltransferase genes in B-cell lymphoma.
GO:0005515 protein binding
IPI
PMID:21397192
Interferon-inducible protein 16: insight into the interactio... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:21397192
Interferon-inducible protein 16: insight into the interaction with tumor suppressor p53.
GO:0005515 protein binding
IPI
PMID:21423215
Camptothecin-induced downregulation of MLL5 contributes to t... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:21423215
Camptothecin-induced downregulation of MLL5 contributes to the activation of tumor suppressor p53.
GO:0005515 protein binding
IPI
PMID:21460856
ATM-mediated phosphorylation activates the tumor-suppressive... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:21460856
ATM-mediated phosphorylation activates the tumor-suppressive function of B56Ξ³-PP2A.
GO:0005515 protein binding
IPI
PMID:21471221
Novel nucleolar pathway connecting intracellular energy stat... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:21471221
2011 Apr 6. Novel nucleolar pathway connecting intracellular energy status with p53 activation.
GO:0005515 protein binding
IPI
PMID:21513714
The ASPP proteins complex and cooperate with p300 to modulat... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:21513714
Epub 2011 Apr 18. The ASPP proteins complex and cooperate with p300 to modulate the transcriptional activity of p53.
GO:0005515 protein binding
IPI
PMID:21625211
COP9 signalosome subunit 6 stabilizes COP1, which functions ... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:21625211
COP9 signalosome subunit 6 stabilizes COP1, which functions as an E3 ubiquitin ligase for 14-3-3Οƒ.
GO:0005515 protein binding
IPI
PMID:21653829
Protein interactome reveals converging molecular pathways am... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:21653829
Protein interactome reveals converging molecular pathways among autism disorders.
GO:0005515 protein binding
IPI
PMID:21670263
Structural and functional characterization of an atypical ac... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:21670263
Structural and functional characterization of an atypical activation domain in erythroid Kruppel-like factor (EKLF).
GO:0005515 protein binding
IPI
PMID:21726810
Caspase-2-mediated cleavage of Mdm2 creates a p53-induced po... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:21726810
Caspase-2-mediated cleavage of Mdm2 creates a p53-induced positive feedback loop.
GO:0005515 protein binding
IPI
PMID:21741598
A Pin1/mutant p53 axis promotes aggressiveness in breast can... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:21741598
A Pin1/mutant p53 axis promotes aggressiveness in breast cancer.
GO:0005515 protein binding
IPI
PMID:21782458
Structural basis of substrate methylation and inhibition of ... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:21782458
Jul 21. Structural basis of substrate methylation and inhibition of SMYD2.
GO:0005515 protein binding
IPI
PMID:21821029
Aurora-A phosphorylates hnRNPK and disrupts its interaction ... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:21821029
2011 Aug 3. Aurora-A phosphorylates hnRNPK and disrupts its interaction with p53.
GO:0005515 protein binding
IPI
PMID:21831840
Wild-type p53 controls cell motility and invasion by dual re... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:21831840
Wild-type p53 controls cell motility and invasion by dual regulation of MET expression.
GO:0005515 protein binding
IPI
PMID:21857681
A stress response pathway regulates DNA damage through Ξ²2-ad... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:21857681
A stress response pathway regulates DNA damage through Ξ²2-adrenoreceptors and Ξ²-arrestin-1.
GO:0005515 protein binding
IPI
PMID:21892170
Structural analysis of the interaction between Hsp90 and the... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:21892170
Structural analysis of the interaction between Hsp90 and the tumor suppressor protein p53.
GO:0005515 protein binding
IPI
PMID:21900206
A directed protein interaction network for investigating int... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:21900206
A directed protein interaction network for investigating intracellular signal transduction.
GO:0005515 protein binding
IPI
PMID:21952639
NIRF constitutes a nodal point in the cell cycle network and... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:21952639
Oct 1. NIRF constitutes a nodal point in the cell cycle network and is a candidate tumor suppressor.
GO:0005515 protein binding
IPI
PMID:21988832
Toward an understanding of the protein interaction network o... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:21988832
Toward an understanding of the protein interaction network of the human liver.
GO:0005515 protein binding
IPI
PMID:22056774
Bilateral inhibition of HAUSP deubiquitinase by a viral inte... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:22056774
Bilateral inhibition of HAUSP deubiquitinase by a viral interferon regulatory factor protein.
GO:0005515 protein binding
IPI
PMID:22085928
Regulation of p53 stability and function by the deubiquitina... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:22085928
Regulation of p53 stability and function by the deubiquitinating enzyme USP42.
GO:0005515 protein binding
IPI
PMID:22103682
Specific domains of nucleolin interact with Hdm2 and antagon... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:22103682
2011 Dec 19. Specific domains of nucleolin interact with Hdm2 and antagonize Hdm2-mediated p53 ubiquitination.
GO:0005515 protein binding
IPI
PMID:22124327
Positive regulation of p53 stability and activity by the deu... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:22124327
Positive regulation of p53 stability and activity by the deubiquitinating enzyme Otubain 1.
GO:0005515 protein binding
IPI
PMID:22265415
Mutant p53 disrupts mammary tissue architecture via the meva... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:22265415
Mutant p53 disrupts mammary tissue architecture via the mevalonate pathway.
GO:0005515 protein binding
IPI
PMID:22340593
Aurora kinase-A inactivates DNA damage-induced apoptosis and... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:22340593
Aurora kinase-A inactivates DNA damage-induced apoptosis and spindle assembly checkpoint response functions of p73.
GO:0005515 protein binding
IPI
PMID:22451927
Role of the translationally controlled tumor protein in DNA ... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:22451927
Role of the translationally controlled tumor protein in DNA damage sensing and repair.
GO:0005515 protein binding
IPI
PMID:22499945
Atg7 modulates p53 activity to regulate cell cycle and survi... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:22499945
Atg7 modulates p53 activity to regulate cell cycle and survival during metabolic stress.
GO:0005515 protein binding
IPI
PMID:22510990
AKT-dependent phosphorylation of Niban regulates nucleophosm... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:22510990
AKT-dependent phosphorylation of Niban regulates nucleophosmin- and MDM2-mediated p53 stability and cell apoptosis.
GO:0005515 protein binding
IPI
PMID:22575647
An autoregulatory feedback loop between Mdm2 and SHP that fi... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:22575647
Epub 2012 Mar 23. An autoregulatory feedback loop between Mdm2 and SHP that fine tunes Mdm2 and SHP stability.
GO:0005515 protein binding
IPI
PMID:22653443
Mutant p53 interactome identifies nardilysin as a p53R273H-s... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:22653443
Mutant p53 interactome identifies nardilysin as a p53R273H-specific binding partner that promotes invasion.
GO:0005515 protein binding
IPI
PMID:22659184
Mutational analysis reveals a dual role of Mdm2 acidic domai... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:22659184
Epub 2012 May 31. Mutational analysis reveals a dual role of Mdm2 acidic domain in the regulation of p53 stability.
GO:0005515 protein binding
IPI
PMID:22723347
Differentiated embryo-chondrocyte expressed gene 1 regulates... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:22723347
Differentiated embryo-chondrocyte expressed gene 1 regulates p53-dependent cell survival versus cell death through macrophage inhibitory cytokine-1.
GO:0005515 protein binding
IPI
PMID:22726440
p53 opens the mitochondrial permeability transition pore to ... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:22726440
p53 opens the mitochondrial permeability transition pore to trigger necrosis.
GO:0005515 protein binding
IPI
PMID:22810585
Viral immune modulators perturb the human molecular network ... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:22810585
Viral immune modulators perturb the human molecular network by common and unique strategies.
GO:0005515 protein binding
IPI
PMID:22810586
Interpreting cancer genomes using systematic host network pe... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:22810586
Interpreting cancer genomes using systematic host network perturbations by tumour virus proteins.
GO:0005515 protein binding
IPI
PMID:22819825
TRIAD1 inhibits MDM2-mediated p53 ubiquitination and degrada... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:22819825
Epub 2012 Jul 20. TRIAD1 inhibits MDM2-mediated p53 ubiquitination and degradation.
GO:0005515 protein binding
IPI
PMID:22975381
A "twist box" code of p53 inactivation: twist box: p53 inter... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:22975381
A "twist box" code of p53 inactivation: twist box: p53 interaction promotes p53 degradation.
GO:0005515 protein binding
IPI
PMID:23010591
Structural features of human histone acetyltransferase p300 ... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:23010591
2012 Sep 23. Structural features of human histone acetyltransferase p300 and its complex with p53.
GO:0005515 protein binding
IPI
PMID:23063560
HMGB1-facilitated p53 DNA binding occurs via HMG-Box/p53 tra... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:23063560
2012 Oct 11. HMGB1-facilitated p53 DNA binding occurs via HMG-Box/p53 transactivation domain interaction, regulated by the acidic tail.
GO:0005515 protein binding
IPI
PMID:23092970
SUMOylation of hnRNP-K is required for p53-mediated cell-cyc... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:23092970
SUMOylation of hnRNP-K is required for p53-mediated cell-cycle arrest in response to DNA damage.
GO:0005515 protein binding
IPI
PMID:23320542
Evidence for self-association of the alternative sigma facto... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:23320542
Evidence for self-association of the alternative sigma factor Οƒ54.
GO:0005515 protein binding
IPI
PMID:23576507
Rotavirus-encoded nonstructural protein 1 modulates cellular... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:23576507
Rotavirus-encoded nonstructural protein 1 modulates cellular apoptotic machinery by targeting tumor suppressor protein p53.
GO:0005515 protein binding
IPI
PMID:23623661
Restoring p53 function in human melanoma cells by inhibiting... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:23623661
2013 Apr 25. Restoring p53 function in human melanoma cells by inhibiting MDM2 and cyclin B1/CDK1-phosphorylated nuclear iASPP.
GO:0005515 protein binding
IPI
PMID:23734815
Fludarabine treatment favors the retention of miR-485-3p by ... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:23734815
Fludarabine treatment favors the retention of miR-485-3p by prostate cancer cells: implications for survival.
GO:0005515 protein binding
IPI
PMID:23752197
S100A4 interacts with p53 in the nucleus and promotes p53 de... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:23752197
S100A4 interacts with p53 in the nucleus and promotes p53 degradation.
GO:0005515 protein binding
IPI
PMID:23776060
Identification of a second Nutlin-3 responsive interaction s... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:23776060
Identification of a second Nutlin-3 responsive interaction site in the N-terminal domain of MDM2 using hydrogen/deuterium exchange mass spectrometry.
GO:0005515 protein binding
IPI
PMID:23870121
SET1 and p300 act synergistically, through coupled histone m... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:23870121
SET1 and p300 act synergistically, through coupled histone modifications, in transcriptional activation by p53.
GO:0005515 protein binding
IPI
PMID:24207125
Transient protein states in designing inhibitors of the MDM2... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:24207125
2013 Oct 24. Transient protein states in designing inhibitors of the MDM2-p53 interaction.
GO:0005515 protein binding
IPI
PMID:24219989
Acetylation of p53 stimulates miRNA processing and determine... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:24219989
Nov 12. Acetylation of p53 stimulates miRNA processing and determines cell survival following genotoxic stress.
GO:0005515 protein binding
IPI
PMID:24380853
The aberrant expression and localization of prohibitin durin... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:24380853
2013 Dec 28. The aberrant expression and localization of prohibitin during apoptosis of human cholangiocarcinoma Mz-ChA-1 cells.
GO:0005515 protein binding
IPI
PMID:24449765
Activation of p53 transcriptional activity by SMRT: a histon... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:24449765
Jan 21. Activation of p53 transcriptional activity by SMRT: a histone deacetylase 3-independent function of a transcriptional corepressor.
GO:0005515 protein binding
IPI
PMID:24492002
VRK1 interacts with p53 forming a basal complex that is acti... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:24492002
2014 Jan 31. VRK1 interacts with p53 forming a basal complex that is activated by UV-induced DNA damage.
GO:0005515 protein binding
IPI
PMID:24667498
JMJD6 promotes colon carcinogenesis through negative regulat... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:24667498
eCollection 2014 Mar.
GO:0005515 protein binding
IPI
PMID:24722188
Protein interaction network of alternatively spliced isoform... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:24722188
Protein interaction network of alternatively spliced isoforms from brain links genetic risk factors for autism.
GO:0005515 protein binding
IPI
PMID:24814347
The DNA-binding domain mediates both nuclear and cytosolic f... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:24814347
May 11. The DNA-binding domain mediates both nuclear and cytosolic functions of p53.
GO:0005515 protein binding
IPI
PMID:25241761
Using an in situ proximity ligation assay to systematically ... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:25241761
Oct 9. Using an in situ proximity ligation assay to systematically profile endogenous protein-protein interactions in a pathway network.
GO:0005515 protein binding
IPI
PMID:25314079
Acetylation of snail modulates the cytokinome of cancer cell... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:25314079
Acetylation of snail modulates the cytokinome of cancer cells to enhance the recruitment of macrophages.
GO:0005515 protein binding
IPI
PMID:25402006
In silico prediction of physical protein interactions and ch... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:25402006
In silico prediction of physical protein interactions and characterization of interactome orphans.
GO:0005515 protein binding
IPI
PMID:25502805
A massively parallel pipeline to clone DNA variants and exam... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:25502805
eCollection 2014 Dec.
GO:0005515 protein binding
IPI
PMID:25579814
Structural plasticity of methyllysine recognition by the tan... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:25579814
Jan 8. Structural plasticity of methyllysine recognition by the tandem tudor domain of 53BP1.
GO:0005515 protein binding
IPI
PMID:25609649
Proteomic analyses reveal distinct chromatin-associated and ... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:25609649
Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes.
GO:0005515 protein binding
IPI
PMID:25651062
An acetyl-methyl switch drives a conformational change in p5... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:25651062
An acetyl-methyl switch drives a conformational change in p53.
GO:0005515 protein binding
IPI
PMID:25670079
14-3-3ΞΆ turns TGF-Ξ²'s function from tumor suppressor to meta... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:25670079
14-3-3ΞΆ turns TGF-Ξ²'s function from tumor suppressor to metastasis promoter in breast cancer by contextual changes of Smad partners from p53 to Gli2.
GO:0005515 protein binding
IPI
PMID:25837623
Regulation of protein quality control by UBE4B and LSD1 thro... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:25837623
eCollection 2015 Apr.
GO:0005515 protein binding
IPI
PMID:25857266
Pontin, a new mutant p53-binding protein, promotes gain-of-f... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:25857266
2015 Apr 10. Pontin, a new mutant p53-binding protein, promotes gain-of-function of mutant p53.
GO:0005515 protein binding
IPI
PMID:26302407
Combined CSL and p53 downregulation promotes cancer-associat... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:26302407
Combined CSL and p53 downregulation promotes cancer-associated fibroblast activation.
GO:0005515 protein binding
IPI
PMID:26331536
Gain-of-function p53 mutants co-opt chromatin pathways to dr... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:26331536
Gain-of-function p53 mutants co-opt chromatin pathways to drive cancer growth.
GO:0005515 protein binding
IPI
PMID:26789255
Structure of the E6/E6AP/p53 complex required for HPV-mediat... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:26789255
Structure of the E6/E6AP/p53 complex required for HPV-mediated degradation of p53.
GO:0005515 protein binding
IPI
PMID:27107012
Pooled-matrix protein interaction screens using Barcode Fusi... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:27107012
Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
GO:0005515 protein binding
IPI
PMID:27519799
p53 down-regulates SARS coronavirus replication and is targe... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:27519799
p53 down-regulates SARS coronavirus replication and is targeted by the SARS-unique domain and PLpro via E3 ubiquitin ligase RCHY1.
GO:0005515 protein binding
IPI
PMID:27605672
Mitogen-Activated Protein Kinase Kinase 2, a Novel E2-Intera... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:27605672
Nov 15. Mitogen-Activated Protein Kinase Kinase 2, a Novel E2-Interacting Protein, Promotes the Growth of Classical Swine Fever Virus via Attenuation of the JAK-STAT Signaling Pathway.
GO:0005515 protein binding
IPI
PMID:29187402
The E3 Ligase RING1 Targets p53 for Degradation and Promotes... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:29187402
Epub 2017 Nov 29. The E3 Ligase RING1 Targets p53 for Degradation and Promotes Cancer Cell Proliferation and Survival.
GO:0005515 protein binding
IPI
PMID:29340707
VRK1 and AURKB form a complex that cross inhibit their kinas... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:29340707
Epub 2018 Jan 16. VRK1 and AURKB form a complex that cross inhibit their kinase activity and the phosphorylation of histone H3 in the progression of mitosis.
GO:0005515 protein binding
IPI
PMID:29997244
LuTHy: a double-readout bioluminescence-based two-hybrid tec... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:29997244
LuTHy: a double-readout bioluminescence-based two-hybrid technology for quantitative mapping of protein-protein interactions in mammalian cells.
GO:0005515 protein binding
IPI
PMID:31467278
Maximizing binary interactome mapping with a minimal number ... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:31467278
Maximizing binary interactome mapping with a minimal number of assays.
GO:0005515 protein binding
IPI
PMID:31511497
miR-146a attenuates apoptosis and modulates autophagy by tar... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:31511497
miR-146a attenuates apoptosis and modulates autophagy by targeting TAF9b/P53 pathway in doxorubicin-induced cardiotoxicity.
GO:0005515 protein binding
IPI
PMID:31515488
Extensive disruption of protein interactions by genetic vari... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:31515488
Extensive disruption of protein interactions by genetic variants across the allele frequency spectrum in human populations.
GO:0005515 protein binding
IPI
PMID:31837246
High-throughput competitive fluorescence polarization assay ... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:31837246
High-throughput competitive fluorescence polarization assay reveals functional redundancy in the S100 protein family.
GO:0005515 protein binding
IPI
PMID:32606738
P55PIK Regulates P53-Dependent Apoptosis in Cancer Cells by ... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:32606738
eCollection 2020. P55PIK Regulates P53-Dependent Apoptosis in Cancer Cells by Interacting with P53 DNA-Specific Domain.
GO:0005515 protein binding
IPI
PMID:32814053
Interactome Mapping Provides a Network of Neurodegenerative ... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:32814053
Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.
GO:0005515 protein binding
IPI
PMID:33961781
Dual proteome-scale networks reveal cell-specific remodeling... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:33961781
2021 May 6. Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
GO:0005515 protein binding
IPI
PMID:34316702
Elucidation of the BMI1 interactome identifies novel regulat... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:34316702
2021 Mar. Elucidation of the BMI1 interactome identifies novel regulatory roles in glioblastoma.
GO:0005515 protein binding
IPI
PMID:34591612
A protein interaction landscape of breast cancer. 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:34591612
Oct 1. A protein interaction landscape of breast cancer.
GO:0005515 protein binding
IPI
PMID:34591642
A protein network map of head and neck cancer reveals PIK3CA... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:34591642
Oct 1. A protein network map of head and neck cancer reveals PIK3CA mutant drug sensitivity.
GO:0005515 protein binding
IPI
PMID:35044719
Proteome-scale mapping of binding sites in the unstructured ... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:35044719
Proteome-scale mapping of binding sites in the unstructured regions of the human proteome.
GO:0005515 protein binding
IPI
PMID:35140242
Human transcription factor protein interaction networks. 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:35140242
Human transcription factor protein interaction networks.
GO:0005515 protein binding
IPI
PMID:35271311
OpenCell: Endogenous tagging for the cartography of human ce... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:35271311
2022 Mar 11. OpenCell: Endogenous tagging for the cartography of human cellular organization.
GO:0005515 protein binding
IPI
PMID:35512704
Systematic discovery of mutation-directed neo-protein-protei... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:35512704
2022 May 4. Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.
GO:0005515 protein binding
IPI
PMID:36931259
A central chaperone-like role for 14-3-3 proteins in human c... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:36931259
A central chaperone-like role for 14-3-3 proteins in human cells.
GO:0005515 protein binding
IPI
PMID:39009827
Proteome-scale characterisation of motif-based interactome r... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:39009827
2024 Jul 15. Proteome-scale characterisation of motif-based interactome rewiring by disease mutations.
GO:0005515 protein binding
IPI
PMID:7799929
Two domains of p53 interact with the TATA-binding protein, a... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:7799929
Two domains of p53 interact with the TATA-binding protein, and the adenovirus 13S E1A protein disrupts the association, relieving p53-mediated transcriptional repression.
GO:0005515 protein binding
IPI
PMID:8207801
Inhibition of p53 DNA binding by human papillomavirus E6 pro... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:8207801
Inhibition of p53 DNA binding by human papillomavirus E6 proteins.
GO:0005515 protein binding
IPI
PMID:8344494
Identification of mutations in p53 that affect its binding t... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:8344494
Identification of mutations in p53 that affect its binding to SV40 large T antigen by using the yeast two-hybrid system.
GO:0005515 protein binding
IPI
PMID:8875926
Structure of the p53 tumor suppressor bound to the ankyrin a... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:8875926
Structure of the p53 tumor suppressor bound to the ankyrin and SH3 domains of 53BP2.
GO:0005515 protein binding
IPI
PMID:8875929
Structure of the MDM2 oncoprotein bound to the p53 tumor sup... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:8875929
Structure of the MDM2 oncoprotein bound to the p53 tumor suppressor transactivation domain.
GO:0005515 protein binding
IPI
PMID:9050995
Identification of p53 unbound to T-antigen in human cells tr... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:9050995
Identification of p53 unbound to T-antigen in human cells transformed by simian virus 40 T-antigen.
GO:0005515 protein binding
IPI
PMID:9188558
Nuclear localization of the NS3 protein of hepatitis C virus... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:9188558
Nuclear localization of the NS3 protein of hepatitis C virus and factors affecting the localization.
GO:0005515 protein binding
IPI
PMID:9194564
Synergistic activation of transcription by CBP and p53. 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:9194564
Synergistic activation of transcription by CBP and p53.
GO:0005515 protein binding
IPI
PMID:9380510
Interaction of p53 with the human Rad51 protein. 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:9380510
Interaction of p53 with the human Rad51 protein.
GO:0005515 protein binding
IPI
PMID:9472015
High mobility group protein-1 (HMG-1) is a unique activator ... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:9472015
High mobility group protein-1 (HMG-1) is a unique activator of p53.
GO:0005515 protein binding
IPI
PMID:9807817
An Arabidopsis immunophilin, AtFKBP12, binds to AtFIP37 (FKB... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:9807817
An Arabidopsis immunophilin, AtFKBP12, binds to AtFIP37 (FKBP interacting protein) in an interaction that is disrupted by FK506.
GO:0005515 protein binding
IPI
PMID:9827557
Complex formation of the nonstructural protein 3 of hepatiti... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:9827557
Complex formation of the nonstructural protein 3 of hepatitis C virus with the p53 tumor suppressor.
GO:0042802 identical protein binding
IPI
PMID:10876243
Structure of the negative regulatory domain of p53 bound to ... 		ACCEPT
Summary: p53 binds to itself to form tetramers through its oligomerization domain, essential for DNA binding.
Reason: Core structural feature - p53 tetramerization through identical protein binding is essential for function.
Supporting Evidence:
PDB:1C26
Crystal structure of p53 tetramerization domain
PMID:10876243
Structure of the negative regulatory domain of p53 bound to S100B(betabeta).
PMID:14759370
Structural mechanism of the bromodomain of the coactivator CBP in p53 transcriptional activation.
PMID:14985081
Hepatitis C virus core protein interacts with p53-binding protein, 53BP2/Bbp/ASPP2, and inhibits p53-mediated apoptosis.
PMID:16291740
2005 Nov 15. Charge modification at multiple C-terminal lysine residues regulates p53 oligomerization and its nucleus-cytoplasm trafficking.
PMID:16461914
Core domain interactions in full-length p53 in solution.
PMID:17612295
Yeast split-ubiquitin-based cytosolic screening system to detect interactions between transcriptionally active proteins.
PMID:17620598
Quaternary structures of tumor suppressor p53 and a specific p53 DNA complex.
PMID:18087040
Regulation of p53 tetramerization and nuclear export by ARC.
PMID:19339993
Crosstalk between sumoylation and acetylation regulates p53-dependent chromatin transcription and DNA binding.
PMID:19667193
Ultraslow oligomerization equilibria of p53 and its implications.
PMID:20004160
Averaging of electron subtomograms and random conical tilt reconstructions through likelihood optimization.
PMID:20159469
Crystal structure of the p53 core domain bound to a full consensus site as a self-assembled tetramer.
PMID:20364130
Apr 4. Diversity in DNA recognition by p53 revealed by crystal structures with Hoogsteen base pairs.
PMID:21178074
Electron microscopy studies on the quaternary structure of p53 reveal different binding modes for p53 tetramers in complex with DNA.
PMID:21522129
An induced fit mechanism regulates p53 DNA binding kinetics to confer sequence specificity.
PMID:21988832
Toward an understanding of the protein interaction network of the human liver.
PMID:22653443
Mutant p53 interactome identifies nardilysin as a p53R273H-specific binding partner that promotes invasion.
PMID:22972749
Domain-domain interactions in full-length p53 and a specific DNA complex probed by methyl NMR spectroscopy.
PMID:25402006
In silico prediction of physical protein interactions and characterization of interactome orphans.
PMID:25609649
Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes.
PMID:31837246
High-throughput competitive fluorescence polarization assay reveals functional redundancy in the S100 protein family.
PMID:35512704
2022 May 4. Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.
PMID:15629713
Binding of natively unfolded HIF-1alpha ODD domain to p53.
PMID:19011621
Arginine methylation regulates the p53 response.
GO:0042802 identical protein binding
IPI
PMID:14759370
Structural mechanism of the bromodomain of the coactivator C... 		ACCEPT
Summary: p53 binds to itself to form tetramers through its oligomerization domain, essential for DNA binding.
Reason: Core structural feature - p53 tetramerization through identical protein binding is essential for function.
Supporting Evidence:
PDB:1C26
Crystal structure of p53 tetramerization domain
PMID:10876243
Structure of the negative regulatory domain of p53 bound to S100B(betabeta).
PMID:14759370
Structural mechanism of the bromodomain of the coactivator CBP in p53 transcriptional activation.
PMID:14985081
Hepatitis C virus core protein interacts with p53-binding protein, 53BP2/Bbp/ASPP2, and inhibits p53-mediated apoptosis.
PMID:16291740
2005 Nov 15. Charge modification at multiple C-terminal lysine residues regulates p53 oligomerization and its nucleus-cytoplasm trafficking.
PMID:16461914
Core domain interactions in full-length p53 in solution.
PMID:17612295
Yeast split-ubiquitin-based cytosolic screening system to detect interactions between transcriptionally active proteins.
PMID:17620598
Quaternary structures of tumor suppressor p53 and a specific p53 DNA complex.
PMID:18087040
Regulation of p53 tetramerization and nuclear export by ARC.
PMID:19339993
Crosstalk between sumoylation and acetylation regulates p53-dependent chromatin transcription and DNA binding.
PMID:19667193
Ultraslow oligomerization equilibria of p53 and its implications.
PMID:20004160
Averaging of electron subtomograms and random conical tilt reconstructions through likelihood optimization.
PMID:20159469
Crystal structure of the p53 core domain bound to a full consensus site as a self-assembled tetramer.
PMID:20364130
Apr 4. Diversity in DNA recognition by p53 revealed by crystal structures with Hoogsteen base pairs.
PMID:21178074
Electron microscopy studies on the quaternary structure of p53 reveal different binding modes for p53 tetramers in complex with DNA.
PMID:21522129
An induced fit mechanism regulates p53 DNA binding kinetics to confer sequence specificity.
PMID:21988832
Toward an understanding of the protein interaction network of the human liver.
PMID:22653443
Mutant p53 interactome identifies nardilysin as a p53R273H-specific binding partner that promotes invasion.
PMID:22972749
Domain-domain interactions in full-length p53 and a specific DNA complex probed by methyl NMR spectroscopy.
PMID:25402006
In silico prediction of physical protein interactions and characterization of interactome orphans.
PMID:25609649
Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes.
PMID:31837246
High-throughput competitive fluorescence polarization assay reveals functional redundancy in the S100 protein family.
PMID:35512704
2022 May 4. Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.
PMID:15629713
Binding of natively unfolded HIF-1alpha ODD domain to p53.
PMID:19011621
Arginine methylation regulates the p53 response.
GO:0042802 identical protein binding
IPI
PMID:14985081
Hepatitis C virus core protein interacts with p53-binding pr... 		ACCEPT
Summary: p53 binds to itself to form tetramers through its oligomerization domain, essential for DNA binding.
Reason: Core structural feature - p53 tetramerization through identical protein binding is essential for function.
Supporting Evidence:
PDB:1C26
Crystal structure of p53 tetramerization domain
PMID:10876243
Structure of the negative regulatory domain of p53 bound to S100B(betabeta).
PMID:14759370
Structural mechanism of the bromodomain of the coactivator CBP in p53 transcriptional activation.
PMID:14985081
Hepatitis C virus core protein interacts with p53-binding protein, 53BP2/Bbp/ASPP2, and inhibits p53-mediated apoptosis.
PMID:16291740
2005 Nov 15. Charge modification at multiple C-terminal lysine residues regulates p53 oligomerization and its nucleus-cytoplasm trafficking.
PMID:16461914
Core domain interactions in full-length p53 in solution.
PMID:17612295
Yeast split-ubiquitin-based cytosolic screening system to detect interactions between transcriptionally active proteins.
PMID:17620598
Quaternary structures of tumor suppressor p53 and a specific p53 DNA complex.
PMID:18087040
Regulation of p53 tetramerization and nuclear export by ARC.
PMID:19339993
Crosstalk between sumoylation and acetylation regulates p53-dependent chromatin transcription and DNA binding.
PMID:19667193
Ultraslow oligomerization equilibria of p53 and its implications.
PMID:20004160
Averaging of electron subtomograms and random conical tilt reconstructions through likelihood optimization.
PMID:20159469
Crystal structure of the p53 core domain bound to a full consensus site as a self-assembled tetramer.
PMID:20364130
Apr 4. Diversity in DNA recognition by p53 revealed by crystal structures with Hoogsteen base pairs.
PMID:21178074
Electron microscopy studies on the quaternary structure of p53 reveal different binding modes for p53 tetramers in complex with DNA.
PMID:21522129
An induced fit mechanism regulates p53 DNA binding kinetics to confer sequence specificity.
PMID:21988832
Toward an understanding of the protein interaction network of the human liver.
PMID:22653443
Mutant p53 interactome identifies nardilysin as a p53R273H-specific binding partner that promotes invasion.
PMID:22972749
Domain-domain interactions in full-length p53 and a specific DNA complex probed by methyl NMR spectroscopy.
PMID:25402006
In silico prediction of physical protein interactions and characterization of interactome orphans.
PMID:25609649
Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes.
PMID:31837246
High-throughput competitive fluorescence polarization assay reveals functional redundancy in the S100 protein family.
PMID:35512704
2022 May 4. Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.
PMID:15629713
Binding of natively unfolded HIF-1alpha ODD domain to p53.
PMID:19011621
Arginine methylation regulates the p53 response.
GO:0042802 identical protein binding
IPI
PMID:16291740
Charge modification at multiple C-terminal lysine residues r... 		ACCEPT
Summary: p53 binds to itself to form tetramers through its oligomerization domain, essential for DNA binding.
Reason: Core structural feature - p53 tetramerization through identical protein binding is essential for function.
Supporting Evidence:
PDB:1C26
Crystal structure of p53 tetramerization domain
PMID:10876243
Structure of the negative regulatory domain of p53 bound to S100B(betabeta).
PMID:14759370
Structural mechanism of the bromodomain of the coactivator CBP in p53 transcriptional activation.
PMID:14985081
Hepatitis C virus core protein interacts with p53-binding protein, 53BP2/Bbp/ASPP2, and inhibits p53-mediated apoptosis.
PMID:16291740
2005 Nov 15. Charge modification at multiple C-terminal lysine residues regulates p53 oligomerization and its nucleus-cytoplasm trafficking.
PMID:16461914
Core domain interactions in full-length p53 in solution.
PMID:17612295
Yeast split-ubiquitin-based cytosolic screening system to detect interactions between transcriptionally active proteins.
PMID:17620598
Quaternary structures of tumor suppressor p53 and a specific p53 DNA complex.
PMID:18087040
Regulation of p53 tetramerization and nuclear export by ARC.
PMID:19339993
Crosstalk between sumoylation and acetylation regulates p53-dependent chromatin transcription and DNA binding.
PMID:19667193
Ultraslow oligomerization equilibria of p53 and its implications.
PMID:20004160
Averaging of electron subtomograms and random conical tilt reconstructions through likelihood optimization.
PMID:20159469
Crystal structure of the p53 core domain bound to a full consensus site as a self-assembled tetramer.
PMID:20364130
Apr 4. Diversity in DNA recognition by p53 revealed by crystal structures with Hoogsteen base pairs.
PMID:21178074
Electron microscopy studies on the quaternary structure of p53 reveal different binding modes for p53 tetramers in complex with DNA.
PMID:21522129
An induced fit mechanism regulates p53 DNA binding kinetics to confer sequence specificity.
PMID:21988832
Toward an understanding of the protein interaction network of the human liver.
PMID:22653443
Mutant p53 interactome identifies nardilysin as a p53R273H-specific binding partner that promotes invasion.
PMID:22972749
Domain-domain interactions in full-length p53 and a specific DNA complex probed by methyl NMR spectroscopy.
PMID:25402006
In silico prediction of physical protein interactions and characterization of interactome orphans.
PMID:25609649
Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes.
PMID:31837246
High-throughput competitive fluorescence polarization assay reveals functional redundancy in the S100 protein family.
PMID:35512704
2022 May 4. Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.
PMID:15629713
Binding of natively unfolded HIF-1alpha ODD domain to p53.
PMID:19011621
Arginine methylation regulates the p53 response.
GO:0042802 identical protein binding
IPI
PMID:16461914
Core domain interactions in full-length p53 in solution. 		ACCEPT
Summary: p53 binds to itself to form tetramers through its oligomerization domain, essential for DNA binding.
Reason: Core structural feature - p53 tetramerization through identical protein binding is essential for function.
Supporting Evidence:
PDB:1C26
Crystal structure of p53 tetramerization domain
PMID:10876243
Structure of the negative regulatory domain of p53 bound to S100B(betabeta).
PMID:14759370
Structural mechanism of the bromodomain of the coactivator CBP in p53 transcriptional activation.
PMID:14985081
Hepatitis C virus core protein interacts with p53-binding protein, 53BP2/Bbp/ASPP2, and inhibits p53-mediated apoptosis.
PMID:16291740
2005 Nov 15. Charge modification at multiple C-terminal lysine residues regulates p53 oligomerization and its nucleus-cytoplasm trafficking.
PMID:16461914
Core domain interactions in full-length p53 in solution.
PMID:17612295
Yeast split-ubiquitin-based cytosolic screening system to detect interactions between transcriptionally active proteins.
PMID:17620598
Quaternary structures of tumor suppressor p53 and a specific p53 DNA complex.
PMID:18087040
Regulation of p53 tetramerization and nuclear export by ARC.
PMID:19339993
Crosstalk between sumoylation and acetylation regulates p53-dependent chromatin transcription and DNA binding.
PMID:19667193
Ultraslow oligomerization equilibria of p53 and its implications.
PMID:20004160
Averaging of electron subtomograms and random conical tilt reconstructions through likelihood optimization.
PMID:20159469
Crystal structure of the p53 core domain bound to a full consensus site as a self-assembled tetramer.
PMID:20364130
Apr 4. Diversity in DNA recognition by p53 revealed by crystal structures with Hoogsteen base pairs.
PMID:21178074
Electron microscopy studies on the quaternary structure of p53 reveal different binding modes for p53 tetramers in complex with DNA.
PMID:21522129
An induced fit mechanism regulates p53 DNA binding kinetics to confer sequence specificity.
PMID:21988832
Toward an understanding of the protein interaction network of the human liver.
PMID:22653443
Mutant p53 interactome identifies nardilysin as a p53R273H-specific binding partner that promotes invasion.
PMID:22972749
Domain-domain interactions in full-length p53 and a specific DNA complex probed by methyl NMR spectroscopy.
PMID:25402006
In silico prediction of physical protein interactions and characterization of interactome orphans.
PMID:25609649
Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes.
PMID:31837246
High-throughput competitive fluorescence polarization assay reveals functional redundancy in the S100 protein family.
PMID:35512704
2022 May 4. Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.
PMID:15629713
Binding of natively unfolded HIF-1alpha ODD domain to p53.
PMID:19011621
Arginine methylation regulates the p53 response.
GO:0042802 identical protein binding
IPI
PMID:17612295
Yeast split-ubiquitin-based cytosolic screening system to de... 		ACCEPT
Summary: p53 binds to itself to form tetramers through its oligomerization domain, essential for DNA binding.
Reason: Core structural feature - p53 tetramerization through identical protein binding is essential for function.
Supporting Evidence:
PDB:1C26
Crystal structure of p53 tetramerization domain
PMID:10876243
Structure of the negative regulatory domain of p53 bound to S100B(betabeta).
PMID:14759370
Structural mechanism of the bromodomain of the coactivator CBP in p53 transcriptional activation.
PMID:14985081
Hepatitis C virus core protein interacts with p53-binding protein, 53BP2/Bbp/ASPP2, and inhibits p53-mediated apoptosis.
PMID:16291740
2005 Nov 15. Charge modification at multiple C-terminal lysine residues regulates p53 oligomerization and its nucleus-cytoplasm trafficking.
PMID:16461914
Core domain interactions in full-length p53 in solution.
PMID:17612295
Yeast split-ubiquitin-based cytosolic screening system to detect interactions between transcriptionally active proteins.
PMID:17620598
Quaternary structures of tumor suppressor p53 and a specific p53 DNA complex.
PMID:18087040
Regulation of p53 tetramerization and nuclear export by ARC.
PMID:19339993
Crosstalk between sumoylation and acetylation regulates p53-dependent chromatin transcription and DNA binding.
PMID:19667193
Ultraslow oligomerization equilibria of p53 and its implications.
PMID:20004160
Averaging of electron subtomograms and random conical tilt reconstructions through likelihood optimization.
PMID:20159469
Crystal structure of the p53 core domain bound to a full consensus site as a self-assembled tetramer.
PMID:20364130
Apr 4. Diversity in DNA recognition by p53 revealed by crystal structures with Hoogsteen base pairs.
PMID:21178074
Electron microscopy studies on the quaternary structure of p53 reveal different binding modes for p53 tetramers in complex with DNA.
PMID:21522129
An induced fit mechanism regulates p53 DNA binding kinetics to confer sequence specificity.
PMID:21988832
Toward an understanding of the protein interaction network of the human liver.
PMID:22653443
Mutant p53 interactome identifies nardilysin as a p53R273H-specific binding partner that promotes invasion.
PMID:22972749
Domain-domain interactions in full-length p53 and a specific DNA complex probed by methyl NMR spectroscopy.
PMID:25402006
In silico prediction of physical protein interactions and characterization of interactome orphans.
PMID:25609649
Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes.
PMID:31837246
High-throughput competitive fluorescence polarization assay reveals functional redundancy in the S100 protein family.
PMID:35512704
2022 May 4. Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.
PMID:15629713
Binding of natively unfolded HIF-1alpha ODD domain to p53.
PMID:19011621
Arginine methylation regulates the p53 response.
GO:0042802 identical protein binding
IPI
PMID:17620598
Quaternary structures of tumor suppressor p53 and a specific... 		ACCEPT
Summary: p53 binds to itself to form tetramers through its oligomerization domain, essential for DNA binding.
Reason: Core structural feature - p53 tetramerization through identical protein binding is essential for function.
Supporting Evidence:
PDB:1C26
Crystal structure of p53 tetramerization domain
PMID:10876243
Structure of the negative regulatory domain of p53 bound to S100B(betabeta).
PMID:14759370
Structural mechanism of the bromodomain of the coactivator CBP in p53 transcriptional activation.
PMID:14985081
Hepatitis C virus core protein interacts with p53-binding protein, 53BP2/Bbp/ASPP2, and inhibits p53-mediated apoptosis.
PMID:16291740
2005 Nov 15. Charge modification at multiple C-terminal lysine residues regulates p53 oligomerization and its nucleus-cytoplasm trafficking.
PMID:16461914
Core domain interactions in full-length p53 in solution.
PMID:17612295
Yeast split-ubiquitin-based cytosolic screening system to detect interactions between transcriptionally active proteins.
PMID:17620598
Quaternary structures of tumor suppressor p53 and a specific p53 DNA complex.
PMID:18087040
Regulation of p53 tetramerization and nuclear export by ARC.
PMID:19339993
Crosstalk between sumoylation and acetylation regulates p53-dependent chromatin transcription and DNA binding.
PMID:19667193
Ultraslow oligomerization equilibria of p53 and its implications.
PMID:20004160
Averaging of electron subtomograms and random conical tilt reconstructions through likelihood optimization.
PMID:20159469
Crystal structure of the p53 core domain bound to a full consensus site as a self-assembled tetramer.
PMID:20364130
Apr 4. Diversity in DNA recognition by p53 revealed by crystal structures with Hoogsteen base pairs.
PMID:21178074
Electron microscopy studies on the quaternary structure of p53 reveal different binding modes for p53 tetramers in complex with DNA.
PMID:21522129
An induced fit mechanism regulates p53 DNA binding kinetics to confer sequence specificity.
PMID:21988832
Toward an understanding of the protein interaction network of the human liver.
PMID:22653443
Mutant p53 interactome identifies nardilysin as a p53R273H-specific binding partner that promotes invasion.
PMID:22972749
Domain-domain interactions in full-length p53 and a specific DNA complex probed by methyl NMR spectroscopy.
PMID:25402006
In silico prediction of physical protein interactions and characterization of interactome orphans.
PMID:25609649
Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes.
PMID:31837246
High-throughput competitive fluorescence polarization assay reveals functional redundancy in the S100 protein family.
PMID:35512704
2022 May 4. Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.
PMID:15629713
Binding of natively unfolded HIF-1alpha ODD domain to p53.
PMID:19011621
Arginine methylation regulates the p53 response.
GO:0042802 identical protein binding
IPI
PMID:18087040
Regulation of p53 tetramerization and nuclear export by ARC. 		ACCEPT
Summary: p53 binds to itself to form tetramers through its oligomerization domain, essential for DNA binding.
Reason: Core structural feature - p53 tetramerization through identical protein binding is essential for function.
Supporting Evidence:
PDB:1C26
Crystal structure of p53 tetramerization domain
PMID:10876243
Structure of the negative regulatory domain of p53 bound to S100B(betabeta).
PMID:14759370
Structural mechanism of the bromodomain of the coactivator CBP in p53 transcriptional activation.
PMID:14985081
Hepatitis C virus core protein interacts with p53-binding protein, 53BP2/Bbp/ASPP2, and inhibits p53-mediated apoptosis.
PMID:16291740
2005 Nov 15. Charge modification at multiple C-terminal lysine residues regulates p53 oligomerization and its nucleus-cytoplasm trafficking.
PMID:16461914
Core domain interactions in full-length p53 in solution.
PMID:17612295
Yeast split-ubiquitin-based cytosolic screening system to detect interactions between transcriptionally active proteins.
PMID:17620598
Quaternary structures of tumor suppressor p53 and a specific p53 DNA complex.
PMID:18087040
Regulation of p53 tetramerization and nuclear export by ARC.
PMID:19339993
Crosstalk between sumoylation and acetylation regulates p53-dependent chromatin transcription and DNA binding.
PMID:19667193
Ultraslow oligomerization equilibria of p53 and its implications.
PMID:20004160
Averaging of electron subtomograms and random conical tilt reconstructions through likelihood optimization.
PMID:20159469
Crystal structure of the p53 core domain bound to a full consensus site as a self-assembled tetramer.
PMID:20364130
Apr 4. Diversity in DNA recognition by p53 revealed by crystal structures with Hoogsteen base pairs.
PMID:21178074
Electron microscopy studies on the quaternary structure of p53 reveal different binding modes for p53 tetramers in complex with DNA.
PMID:21522129
An induced fit mechanism regulates p53 DNA binding kinetics to confer sequence specificity.
PMID:21988832
Toward an understanding of the protein interaction network of the human liver.
PMID:22653443
Mutant p53 interactome identifies nardilysin as a p53R273H-specific binding partner that promotes invasion.
PMID:22972749
Domain-domain interactions in full-length p53 and a specific DNA complex probed by methyl NMR spectroscopy.
PMID:25402006
In silico prediction of physical protein interactions and characterization of interactome orphans.
PMID:25609649
Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes.
PMID:31837246
High-throughput competitive fluorescence polarization assay reveals functional redundancy in the S100 protein family.
PMID:35512704
2022 May 4. Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.
PMID:15629713
Binding of natively unfolded HIF-1alpha ODD domain to p53.
PMID:19011621
Arginine methylation regulates the p53 response.
GO:0042802 identical protein binding
IPI
PMID:19339993
Crosstalk between sumoylation and acetylation regulates p53-... 		ACCEPT
Summary: p53 binds to itself to form tetramers through its oligomerization domain, essential for DNA binding.
Reason: Core structural feature - p53 tetramerization through identical protein binding is essential for function.
Supporting Evidence:
PDB:1C26
Crystal structure of p53 tetramerization domain
PMID:10876243
Structure of the negative regulatory domain of p53 bound to S100B(betabeta).
PMID:14759370
Structural mechanism of the bromodomain of the coactivator CBP in p53 transcriptional activation.
PMID:14985081
Hepatitis C virus core protein interacts with p53-binding protein, 53BP2/Bbp/ASPP2, and inhibits p53-mediated apoptosis.
PMID:16291740
2005 Nov 15. Charge modification at multiple C-terminal lysine residues regulates p53 oligomerization and its nucleus-cytoplasm trafficking.
PMID:16461914
Core domain interactions in full-length p53 in solution.
PMID:17612295
Yeast split-ubiquitin-based cytosolic screening system to detect interactions between transcriptionally active proteins.
PMID:17620598
Quaternary structures of tumor suppressor p53 and a specific p53 DNA complex.
PMID:18087040
Regulation of p53 tetramerization and nuclear export by ARC.
PMID:19339993
Crosstalk between sumoylation and acetylation regulates p53-dependent chromatin transcription and DNA binding.
PMID:19667193
Ultraslow oligomerization equilibria of p53 and its implications.
PMID:20004160
Averaging of electron subtomograms and random conical tilt reconstructions through likelihood optimization.
PMID:20159469
Crystal structure of the p53 core domain bound to a full consensus site as a self-assembled tetramer.
PMID:20364130
Apr 4. Diversity in DNA recognition by p53 revealed by crystal structures with Hoogsteen base pairs.
PMID:21178074
Electron microscopy studies on the quaternary structure of p53 reveal different binding modes for p53 tetramers in complex with DNA.
PMID:21522129
An induced fit mechanism regulates p53 DNA binding kinetics to confer sequence specificity.
PMID:21988832
Toward an understanding of the protein interaction network of the human liver.
PMID:22653443
Mutant p53 interactome identifies nardilysin as a p53R273H-specific binding partner that promotes invasion.
PMID:22972749
Domain-domain interactions in full-length p53 and a specific DNA complex probed by methyl NMR spectroscopy.
PMID:25402006
In silico prediction of physical protein interactions and characterization of interactome orphans.
PMID:25609649
Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes.
PMID:31837246
High-throughput competitive fluorescence polarization assay reveals functional redundancy in the S100 protein family.
PMID:35512704
2022 May 4. Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.
PMID:15629713
Binding of natively unfolded HIF-1alpha ODD domain to p53.
PMID:19011621
Arginine methylation regulates the p53 response.
GO:0042802 identical protein binding
IPI
PMID:19667193
Ultraslow oligomerization equilibria of p53 and its implicat... 		ACCEPT
Summary: p53 binds to itself to form tetramers through its oligomerization domain, essential for DNA binding.
Reason: Core structural feature - p53 tetramerization through identical protein binding is essential for function.
Supporting Evidence:
PDB:1C26
Crystal structure of p53 tetramerization domain
PMID:10876243
Structure of the negative regulatory domain of p53 bound to S100B(betabeta).
PMID:14759370
Structural mechanism of the bromodomain of the coactivator CBP in p53 transcriptional activation.
PMID:14985081
Hepatitis C virus core protein interacts with p53-binding protein, 53BP2/Bbp/ASPP2, and inhibits p53-mediated apoptosis.
PMID:16291740
2005 Nov 15. Charge modification at multiple C-terminal lysine residues regulates p53 oligomerization and its nucleus-cytoplasm trafficking.
PMID:16461914
Core domain interactions in full-length p53 in solution.
PMID:17612295
Yeast split-ubiquitin-based cytosolic screening system to detect interactions between transcriptionally active proteins.
PMID:17620598
Quaternary structures of tumor suppressor p53 and a specific p53 DNA complex.
PMID:18087040
Regulation of p53 tetramerization and nuclear export by ARC.
PMID:19339993
Crosstalk between sumoylation and acetylation regulates p53-dependent chromatin transcription and DNA binding.
PMID:19667193
Ultraslow oligomerization equilibria of p53 and its implications.
PMID:20004160
Averaging of electron subtomograms and random conical tilt reconstructions through likelihood optimization.
PMID:20159469
Crystal structure of the p53 core domain bound to a full consensus site as a self-assembled tetramer.
PMID:20364130
Apr 4. Diversity in DNA recognition by p53 revealed by crystal structures with Hoogsteen base pairs.
PMID:21178074
Electron microscopy studies on the quaternary structure of p53 reveal different binding modes for p53 tetramers in complex with DNA.
PMID:21522129
An induced fit mechanism regulates p53 DNA binding kinetics to confer sequence specificity.
PMID:21988832
Toward an understanding of the protein interaction network of the human liver.
PMID:22653443
Mutant p53 interactome identifies nardilysin as a p53R273H-specific binding partner that promotes invasion.
PMID:22972749
Domain-domain interactions in full-length p53 and a specific DNA complex probed by methyl NMR spectroscopy.
PMID:25402006
In silico prediction of physical protein interactions and characterization of interactome orphans.
PMID:25609649
Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes.
PMID:31837246
High-throughput competitive fluorescence polarization assay reveals functional redundancy in the S100 protein family.
PMID:35512704
2022 May 4. Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.
PMID:15629713
Binding of natively unfolded HIF-1alpha ODD domain to p53.
PMID:19011621
Arginine methylation regulates the p53 response.
GO:0042802 identical protein binding
IPI
PMID:20004160
Averaging of electron subtomograms and random conical tilt r... 		ACCEPT
Summary: p53 binds to itself to form tetramers through its oligomerization domain, essential for DNA binding.
Reason: Core structural feature - p53 tetramerization through identical protein binding is essential for function.
Supporting Evidence:
PDB:1C26
Crystal structure of p53 tetramerization domain
PMID:10876243
Structure of the negative regulatory domain of p53 bound to S100B(betabeta).
PMID:14759370
Structural mechanism of the bromodomain of the coactivator CBP in p53 transcriptional activation.
PMID:14985081
Hepatitis C virus core protein interacts with p53-binding protein, 53BP2/Bbp/ASPP2, and inhibits p53-mediated apoptosis.
PMID:16291740
2005 Nov 15. Charge modification at multiple C-terminal lysine residues regulates p53 oligomerization and its nucleus-cytoplasm trafficking.
PMID:16461914
Core domain interactions in full-length p53 in solution.
PMID:17612295
Yeast split-ubiquitin-based cytosolic screening system to detect interactions between transcriptionally active proteins.
PMID:17620598
Quaternary structures of tumor suppressor p53 and a specific p53 DNA complex.
PMID:18087040
Regulation of p53 tetramerization and nuclear export by ARC.
PMID:19339993
Crosstalk between sumoylation and acetylation regulates p53-dependent chromatin transcription and DNA binding.
PMID:19667193
Ultraslow oligomerization equilibria of p53 and its implications.
PMID:20004160
Averaging of electron subtomograms and random conical tilt reconstructions through likelihood optimization.
PMID:20159469
Crystal structure of the p53 core domain bound to a full consensus site as a self-assembled tetramer.
PMID:20364130
Apr 4. Diversity in DNA recognition by p53 revealed by crystal structures with Hoogsteen base pairs.
PMID:21178074
Electron microscopy studies on the quaternary structure of p53 reveal different binding modes for p53 tetramers in complex with DNA.
PMID:21522129
An induced fit mechanism regulates p53 DNA binding kinetics to confer sequence specificity.
PMID:21988832
Toward an understanding of the protein interaction network of the human liver.
PMID:22653443
Mutant p53 interactome identifies nardilysin as a p53R273H-specific binding partner that promotes invasion.
PMID:22972749
Domain-domain interactions in full-length p53 and a specific DNA complex probed by methyl NMR spectroscopy.
PMID:25402006
In silico prediction of physical protein interactions and characterization of interactome orphans.
PMID:25609649
Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes.
PMID:31837246
High-throughput competitive fluorescence polarization assay reveals functional redundancy in the S100 protein family.
PMID:35512704
2022 May 4. Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.
PMID:15629713
Binding of natively unfolded HIF-1alpha ODD domain to p53.
PMID:19011621
Arginine methylation regulates the p53 response.
GO:0042802 identical protein binding
IPI
PMID:20159469
Crystal structure of the p53 core domain bound to a full con... 		ACCEPT
Summary: p53 binds to itself to form tetramers through its oligomerization domain, essential for DNA binding.
Reason: Core structural feature - p53 tetramerization through identical protein binding is essential for function.
Supporting Evidence:
PDB:1C26
Crystal structure of p53 tetramerization domain
PMID:10876243
Structure of the negative regulatory domain of p53 bound to S100B(betabeta).
PMID:14759370
Structural mechanism of the bromodomain of the coactivator CBP in p53 transcriptional activation.
PMID:14985081
Hepatitis C virus core protein interacts with p53-binding protein, 53BP2/Bbp/ASPP2, and inhibits p53-mediated apoptosis.
PMID:16291740
2005 Nov 15. Charge modification at multiple C-terminal lysine residues regulates p53 oligomerization and its nucleus-cytoplasm trafficking.
PMID:16461914
Core domain interactions in full-length p53 in solution.
PMID:17612295
Yeast split-ubiquitin-based cytosolic screening system to detect interactions between transcriptionally active proteins.
PMID:17620598
Quaternary structures of tumor suppressor p53 and a specific p53 DNA complex.
PMID:18087040
Regulation of p53 tetramerization and nuclear export by ARC.
PMID:19339993
Crosstalk between sumoylation and acetylation regulates p53-dependent chromatin transcription and DNA binding.
PMID:19667193
Ultraslow oligomerization equilibria of p53 and its implications.
PMID:20004160
Averaging of electron subtomograms and random conical tilt reconstructions through likelihood optimization.
PMID:20159469
Crystal structure of the p53 core domain bound to a full consensus site as a self-assembled tetramer.
PMID:20364130
Apr 4. Diversity in DNA recognition by p53 revealed by crystal structures with Hoogsteen base pairs.
PMID:21178074
Electron microscopy studies on the quaternary structure of p53 reveal different binding modes for p53 tetramers in complex with DNA.
PMID:21522129
An induced fit mechanism regulates p53 DNA binding kinetics to confer sequence specificity.
PMID:21988832
Toward an understanding of the protein interaction network of the human liver.
PMID:22653443
Mutant p53 interactome identifies nardilysin as a p53R273H-specific binding partner that promotes invasion.
PMID:22972749
Domain-domain interactions in full-length p53 and a specific DNA complex probed by methyl NMR spectroscopy.
PMID:25402006
In silico prediction of physical protein interactions and characterization of interactome orphans.
PMID:25609649
Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes.
PMID:31837246
High-throughput competitive fluorescence polarization assay reveals functional redundancy in the S100 protein family.
PMID:35512704
2022 May 4. Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.
PMID:15629713
Binding of natively unfolded HIF-1alpha ODD domain to p53.
PMID:19011621
Arginine methylation regulates the p53 response.
GO:0042802 identical protein binding
IPI
PMID:20364130
Diversity in DNA recognition by p53 revealed by crystal stru... 		ACCEPT
Summary: p53 binds to itself to form tetramers through its oligomerization domain, essential for DNA binding.
Reason: Core structural feature - p53 tetramerization through identical protein binding is essential for function.
Supporting Evidence:
PDB:1C26
Crystal structure of p53 tetramerization domain
PMID:10876243
Structure of the negative regulatory domain of p53 bound to S100B(betabeta).
PMID:14759370
Structural mechanism of the bromodomain of the coactivator CBP in p53 transcriptional activation.
PMID:14985081
Hepatitis C virus core protein interacts with p53-binding protein, 53BP2/Bbp/ASPP2, and inhibits p53-mediated apoptosis.
PMID:16291740
2005 Nov 15. Charge modification at multiple C-terminal lysine residues regulates p53 oligomerization and its nucleus-cytoplasm trafficking.
PMID:16461914
Core domain interactions in full-length p53 in solution.
PMID:17612295
Yeast split-ubiquitin-based cytosolic screening system to detect interactions between transcriptionally active proteins.
PMID:17620598
Quaternary structures of tumor suppressor p53 and a specific p53 DNA complex.
PMID:18087040
Regulation of p53 tetramerization and nuclear export by ARC.
PMID:19339993
Crosstalk between sumoylation and acetylation regulates p53-dependent chromatin transcription and DNA binding.
PMID:19667193
Ultraslow oligomerization equilibria of p53 and its implications.
PMID:20004160
Averaging of electron subtomograms and random conical tilt reconstructions through likelihood optimization.
PMID:20159469
Crystal structure of the p53 core domain bound to a full consensus site as a self-assembled tetramer.
PMID:20364130
Apr 4. Diversity in DNA recognition by p53 revealed by crystal structures with Hoogsteen base pairs.
PMID:21178074
Electron microscopy studies on the quaternary structure of p53 reveal different binding modes for p53 tetramers in complex with DNA.
PMID:21522129
An induced fit mechanism regulates p53 DNA binding kinetics to confer sequence specificity.
PMID:21988832
Toward an understanding of the protein interaction network of the human liver.
PMID:22653443
Mutant p53 interactome identifies nardilysin as a p53R273H-specific binding partner that promotes invasion.
PMID:22972749
Domain-domain interactions in full-length p53 and a specific DNA complex probed by methyl NMR spectroscopy.
PMID:25402006
In silico prediction of physical protein interactions and characterization of interactome orphans.
PMID:25609649
Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes.
PMID:31837246
High-throughput competitive fluorescence polarization assay reveals functional redundancy in the S100 protein family.
PMID:35512704
2022 May 4. Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.
PMID:15629713
Binding of natively unfolded HIF-1alpha ODD domain to p53.
PMID:19011621
Arginine methylation regulates the p53 response.
GO:0042802 identical protein binding
IPI
PMID:21178074
Electron microscopy studies on the quaternary structure of p... 		ACCEPT
Summary: p53 binds to itself to form tetramers through its oligomerization domain, essential for DNA binding.
Reason: Core structural feature - p53 tetramerization through identical protein binding is essential for function.
Supporting Evidence:
PDB:1C26
Crystal structure of p53 tetramerization domain
PMID:10876243
Structure of the negative regulatory domain of p53 bound to S100B(betabeta).
PMID:14759370
Structural mechanism of the bromodomain of the coactivator CBP in p53 transcriptional activation.
PMID:14985081
Hepatitis C virus core protein interacts with p53-binding protein, 53BP2/Bbp/ASPP2, and inhibits p53-mediated apoptosis.
PMID:16291740
2005 Nov 15. Charge modification at multiple C-terminal lysine residues regulates p53 oligomerization and its nucleus-cytoplasm trafficking.
PMID:16461914
Core domain interactions in full-length p53 in solution.
PMID:17612295
Yeast split-ubiquitin-based cytosolic screening system to detect interactions between transcriptionally active proteins.
PMID:17620598
Quaternary structures of tumor suppressor p53 and a specific p53 DNA complex.
PMID:18087040
Regulation of p53 tetramerization and nuclear export by ARC.
PMID:19339993
Crosstalk between sumoylation and acetylation regulates p53-dependent chromatin transcription and DNA binding.
PMID:19667193
Ultraslow oligomerization equilibria of p53 and its implications.
PMID:20004160
Averaging of electron subtomograms and random conical tilt reconstructions through likelihood optimization.
PMID:20159469
Crystal structure of the p53 core domain bound to a full consensus site as a self-assembled tetramer.
PMID:20364130
Apr 4. Diversity in DNA recognition by p53 revealed by crystal structures with Hoogsteen base pairs.
PMID:21178074
Electron microscopy studies on the quaternary structure of p53 reveal different binding modes for p53 tetramers in complex with DNA.
PMID:21522129
An induced fit mechanism regulates p53 DNA binding kinetics to confer sequence specificity.
PMID:21988832
Toward an understanding of the protein interaction network of the human liver.
PMID:22653443
Mutant p53 interactome identifies nardilysin as a p53R273H-specific binding partner that promotes invasion.
PMID:22972749
Domain-domain interactions in full-length p53 and a specific DNA complex probed by methyl NMR spectroscopy.
PMID:25402006
In silico prediction of physical protein interactions and characterization of interactome orphans.
PMID:25609649
Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes.
PMID:31837246
High-throughput competitive fluorescence polarization assay reveals functional redundancy in the S100 protein family.
PMID:35512704
2022 May 4. Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.
PMID:15629713
Binding of natively unfolded HIF-1alpha ODD domain to p53.
PMID:19011621
Arginine methylation regulates the p53 response.
GO:0042802 identical protein binding
IPI
PMID:21522129
An induced fit mechanism regulates p53 DNA binding kinetics ... 		ACCEPT
Summary: p53 binds to itself to form tetramers through its oligomerization domain, essential for DNA binding.
Reason: Core structural feature - p53 tetramerization through identical protein binding is essential for function.
Supporting Evidence:
PDB:1C26
Crystal structure of p53 tetramerization domain
PMID:10876243
Structure of the negative regulatory domain of p53 bound to S100B(betabeta).
PMID:14759370
Structural mechanism of the bromodomain of the coactivator CBP in p53 transcriptional activation.
PMID:14985081
Hepatitis C virus core protein interacts with p53-binding protein, 53BP2/Bbp/ASPP2, and inhibits p53-mediated apoptosis.
PMID:16291740
2005 Nov 15. Charge modification at multiple C-terminal lysine residues regulates p53 oligomerization and its nucleus-cytoplasm trafficking.
PMID:16461914
Core domain interactions in full-length p53 in solution.
PMID:17612295
Yeast split-ubiquitin-based cytosolic screening system to detect interactions between transcriptionally active proteins.
PMID:17620598
Quaternary structures of tumor suppressor p53 and a specific p53 DNA complex.
PMID:18087040
Regulation of p53 tetramerization and nuclear export by ARC.
PMID:19339993
Crosstalk between sumoylation and acetylation regulates p53-dependent chromatin transcription and DNA binding.
PMID:19667193
Ultraslow oligomerization equilibria of p53 and its implications.
PMID:20004160
Averaging of electron subtomograms and random conical tilt reconstructions through likelihood optimization.
PMID:20159469
Crystal structure of the p53 core domain bound to a full consensus site as a self-assembled tetramer.
PMID:20364130
Apr 4. Diversity in DNA recognition by p53 revealed by crystal structures with Hoogsteen base pairs.
PMID:21178074
Electron microscopy studies on the quaternary structure of p53 reveal different binding modes for p53 tetramers in complex with DNA.
PMID:21522129
An induced fit mechanism regulates p53 DNA binding kinetics to confer sequence specificity.
PMID:21988832
Toward an understanding of the protein interaction network of the human liver.
PMID:22653443
Mutant p53 interactome identifies nardilysin as a p53R273H-specific binding partner that promotes invasion.
PMID:22972749
Domain-domain interactions in full-length p53 and a specific DNA complex probed by methyl NMR spectroscopy.
PMID:25402006
In silico prediction of physical protein interactions and characterization of interactome orphans.
PMID:25609649
Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes.
PMID:31837246
High-throughput competitive fluorescence polarization assay reveals functional redundancy in the S100 protein family.
PMID:35512704
2022 May 4. Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.
PMID:15629713
Binding of natively unfolded HIF-1alpha ODD domain to p53.
PMID:19011621
Arginine methylation regulates the p53 response.
GO:0042802 identical protein binding
IPI
PMID:21988832
Toward an understanding of the protein interaction network o... 		ACCEPT
Summary: p53 binds to itself to form tetramers through its oligomerization domain, essential for DNA binding.
Reason: Core structural feature - p53 tetramerization through identical protein binding is essential for function.
Supporting Evidence:
PDB:1C26
Crystal structure of p53 tetramerization domain
PMID:10876243
Structure of the negative regulatory domain of p53 bound to S100B(betabeta).
PMID:14759370
Structural mechanism of the bromodomain of the coactivator CBP in p53 transcriptional activation.
PMID:14985081
Hepatitis C virus core protein interacts with p53-binding protein, 53BP2/Bbp/ASPP2, and inhibits p53-mediated apoptosis.
PMID:16291740
2005 Nov 15. Charge modification at multiple C-terminal lysine residues regulates p53 oligomerization and its nucleus-cytoplasm trafficking.
PMID:16461914
Core domain interactions in full-length p53 in solution.
PMID:17612295
Yeast split-ubiquitin-based cytosolic screening system to detect interactions between transcriptionally active proteins.
PMID:17620598
Quaternary structures of tumor suppressor p53 and a specific p53 DNA complex.
PMID:18087040
Regulation of p53 tetramerization and nuclear export by ARC.
PMID:19339993
Crosstalk between sumoylation and acetylation regulates p53-dependent chromatin transcription and DNA binding.
PMID:19667193
Ultraslow oligomerization equilibria of p53 and its implications.
PMID:20004160
Averaging of electron subtomograms and random conical tilt reconstructions through likelihood optimization.
PMID:20159469
Crystal structure of the p53 core domain bound to a full consensus site as a self-assembled tetramer.
PMID:20364130
Apr 4. Diversity in DNA recognition by p53 revealed by crystal structures with Hoogsteen base pairs.
PMID:21178074
Electron microscopy studies on the quaternary structure of p53 reveal different binding modes for p53 tetramers in complex with DNA.
PMID:21522129
An induced fit mechanism regulates p53 DNA binding kinetics to confer sequence specificity.
PMID:21988832
Toward an understanding of the protein interaction network of the human liver.
PMID:22653443
Mutant p53 interactome identifies nardilysin as a p53R273H-specific binding partner that promotes invasion.
PMID:22972749
Domain-domain interactions in full-length p53 and a specific DNA complex probed by methyl NMR spectroscopy.
PMID:25402006
In silico prediction of physical protein interactions and characterization of interactome orphans.
PMID:25609649
Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes.
PMID:31837246
High-throughput competitive fluorescence polarization assay reveals functional redundancy in the S100 protein family.
PMID:35512704
2022 May 4. Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.
PMID:15629713
Binding of natively unfolded HIF-1alpha ODD domain to p53.
PMID:19011621
Arginine methylation regulates the p53 response.
GO:0042802 identical protein binding
IPI
PMID:22653443
Mutant p53 interactome identifies nardilysin as a p53R273H-s... 		ACCEPT
Summary: p53 binds to itself to form tetramers through its oligomerization domain, essential for DNA binding.
Reason: Core structural feature - p53 tetramerization through identical protein binding is essential for function.
Supporting Evidence:
PDB:1C26
Crystal structure of p53 tetramerization domain
PMID:10876243
Structure of the negative regulatory domain of p53 bound to S100B(betabeta).
PMID:14759370
Structural mechanism of the bromodomain of the coactivator CBP in p53 transcriptional activation.
PMID:14985081
Hepatitis C virus core protein interacts with p53-binding protein, 53BP2/Bbp/ASPP2, and inhibits p53-mediated apoptosis.
PMID:16291740
2005 Nov 15. Charge modification at multiple C-terminal lysine residues regulates p53 oligomerization and its nucleus-cytoplasm trafficking.
PMID:16461914
Core domain interactions in full-length p53 in solution.
PMID:17612295
Yeast split-ubiquitin-based cytosolic screening system to detect interactions between transcriptionally active proteins.
PMID:17620598
Quaternary structures of tumor suppressor p53 and a specific p53 DNA complex.
PMID:18087040
Regulation of p53 tetramerization and nuclear export by ARC.
PMID:19339993
Crosstalk between sumoylation and acetylation regulates p53-dependent chromatin transcription and DNA binding.
PMID:19667193
Ultraslow oligomerization equilibria of p53 and its implications.
PMID:20004160
Averaging of electron subtomograms and random conical tilt reconstructions through likelihood optimization.
PMID:20159469
Crystal structure of the p53 core domain bound to a full consensus site as a self-assembled tetramer.
PMID:20364130
Apr 4. Diversity in DNA recognition by p53 revealed by crystal structures with Hoogsteen base pairs.
PMID:21178074
Electron microscopy studies on the quaternary structure of p53 reveal different binding modes for p53 tetramers in complex with DNA.
PMID:21522129
An induced fit mechanism regulates p53 DNA binding kinetics to confer sequence specificity.
PMID:21988832
Toward an understanding of the protein interaction network of the human liver.
PMID:22653443
Mutant p53 interactome identifies nardilysin as a p53R273H-specific binding partner that promotes invasion.
PMID:22972749
Domain-domain interactions in full-length p53 and a specific DNA complex probed by methyl NMR spectroscopy.
PMID:25402006
In silico prediction of physical protein interactions and characterization of interactome orphans.
PMID:25609649
Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes.
PMID:31837246
High-throughput competitive fluorescence polarization assay reveals functional redundancy in the S100 protein family.
PMID:35512704
2022 May 4. Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.
PMID:15629713
Binding of natively unfolded HIF-1alpha ODD domain to p53.
PMID:19011621
Arginine methylation regulates the p53 response.
GO:0042802 identical protein binding
IPI
PMID:22972749
Domain-domain interactions in full-length p53 and a specific... 		ACCEPT
Summary: p53 binds to itself to form tetramers through its oligomerization domain, essential for DNA binding.
Reason: Core structural feature - p53 tetramerization through identical protein binding is essential for function.
Supporting Evidence:
PDB:1C26
Crystal structure of p53 tetramerization domain
PMID:10876243
Structure of the negative regulatory domain of p53 bound to S100B(betabeta).
PMID:14759370
Structural mechanism of the bromodomain of the coactivator CBP in p53 transcriptional activation.
PMID:14985081
Hepatitis C virus core protein interacts with p53-binding protein, 53BP2/Bbp/ASPP2, and inhibits p53-mediated apoptosis.
PMID:16291740
2005 Nov 15. Charge modification at multiple C-terminal lysine residues regulates p53 oligomerization and its nucleus-cytoplasm trafficking.
PMID:16461914
Core domain interactions in full-length p53 in solution.
PMID:17612295
Yeast split-ubiquitin-based cytosolic screening system to detect interactions between transcriptionally active proteins.
PMID:17620598
Quaternary structures of tumor suppressor p53 and a specific p53 DNA complex.
PMID:18087040
Regulation of p53 tetramerization and nuclear export by ARC.
PMID:19339993
Crosstalk between sumoylation and acetylation regulates p53-dependent chromatin transcription and DNA binding.
PMID:19667193
Ultraslow oligomerization equilibria of p53 and its implications.
PMID:20004160
Averaging of electron subtomograms and random conical tilt reconstructions through likelihood optimization.
PMID:20159469
Crystal structure of the p53 core domain bound to a full consensus site as a self-assembled tetramer.
PMID:20364130
Apr 4. Diversity in DNA recognition by p53 revealed by crystal structures with Hoogsteen base pairs.
PMID:21178074
Electron microscopy studies on the quaternary structure of p53 reveal different binding modes for p53 tetramers in complex with DNA.
PMID:21522129
An induced fit mechanism regulates p53 DNA binding kinetics to confer sequence specificity.
PMID:21988832
Toward an understanding of the protein interaction network of the human liver.
PMID:22653443
Mutant p53 interactome identifies nardilysin as a p53R273H-specific binding partner that promotes invasion.
PMID:22972749
Domain-domain interactions in full-length p53 and a specific DNA complex probed by methyl NMR spectroscopy.
PMID:25402006
In silico prediction of physical protein interactions and characterization of interactome orphans.
PMID:25609649
Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes.
PMID:31837246
High-throughput competitive fluorescence polarization assay reveals functional redundancy in the S100 protein family.
PMID:35512704
2022 May 4. Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.
PMID:15629713
Binding of natively unfolded HIF-1alpha ODD domain to p53.
PMID:19011621
Arginine methylation regulates the p53 response.
GO:0042802 identical protein binding
IPI
PMID:25402006
In silico prediction of physical protein interactions and ch... 		ACCEPT
Summary: p53 binds to itself to form tetramers through its oligomerization domain, essential for DNA binding.
Reason: Core structural feature - p53 tetramerization through identical protein binding is essential for function.
Supporting Evidence:
PDB:1C26
Crystal structure of p53 tetramerization domain
PMID:10876243
Structure of the negative regulatory domain of p53 bound to S100B(betabeta).
PMID:14759370
Structural mechanism of the bromodomain of the coactivator CBP in p53 transcriptional activation.
PMID:14985081
Hepatitis C virus core protein interacts with p53-binding protein, 53BP2/Bbp/ASPP2, and inhibits p53-mediated apoptosis.
PMID:16291740
2005 Nov 15. Charge modification at multiple C-terminal lysine residues regulates p53 oligomerization and its nucleus-cytoplasm trafficking.
PMID:16461914
Core domain interactions in full-length p53 in solution.
PMID:17612295
Yeast split-ubiquitin-based cytosolic screening system to detect interactions between transcriptionally active proteins.
PMID:17620598
Quaternary structures of tumor suppressor p53 and a specific p53 DNA complex.
PMID:18087040
Regulation of p53 tetramerization and nuclear export by ARC.
PMID:19339993
Crosstalk between sumoylation and acetylation regulates p53-dependent chromatin transcription and DNA binding.
PMID:19667193
Ultraslow oligomerization equilibria of p53 and its implications.
PMID:20004160
Averaging of electron subtomograms and random conical tilt reconstructions through likelihood optimization.
PMID:20159469
Crystal structure of the p53 core domain bound to a full consensus site as a self-assembled tetramer.
PMID:20364130
Apr 4. Diversity in DNA recognition by p53 revealed by crystal structures with Hoogsteen base pairs.
PMID:21178074
Electron microscopy studies on the quaternary structure of p53 reveal different binding modes for p53 tetramers in complex with DNA.
PMID:21522129
An induced fit mechanism regulates p53 DNA binding kinetics to confer sequence specificity.
PMID:21988832
Toward an understanding of the protein interaction network of the human liver.
PMID:22653443
Mutant p53 interactome identifies nardilysin as a p53R273H-specific binding partner that promotes invasion.
PMID:22972749
Domain-domain interactions in full-length p53 and a specific DNA complex probed by methyl NMR spectroscopy.
PMID:25402006
In silico prediction of physical protein interactions and characterization of interactome orphans.
PMID:25609649
Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes.
PMID:31837246
High-throughput competitive fluorescence polarization assay reveals functional redundancy in the S100 protein family.
PMID:35512704
2022 May 4. Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.
PMID:15629713
Binding of natively unfolded HIF-1alpha ODD domain to p53.
PMID:19011621
Arginine methylation regulates the p53 response.
GO:0042802 identical protein binding
IPI
PMID:25609649
Proteomic analyses reveal distinct chromatin-associated and ... 		ACCEPT
Summary: p53 binds to itself to form tetramers through its oligomerization domain, essential for DNA binding.
Reason: Core structural feature - p53 tetramerization through identical protein binding is essential for function.
Supporting Evidence:
PDB:1C26
Crystal structure of p53 tetramerization domain
PMID:10876243
Structure of the negative regulatory domain of p53 bound to S100B(betabeta).
PMID:14759370
Structural mechanism of the bromodomain of the coactivator CBP in p53 transcriptional activation.
PMID:14985081
Hepatitis C virus core protein interacts with p53-binding protein, 53BP2/Bbp/ASPP2, and inhibits p53-mediated apoptosis.
PMID:16291740
2005 Nov 15. Charge modification at multiple C-terminal lysine residues regulates p53 oligomerization and its nucleus-cytoplasm trafficking.
PMID:16461914
Core domain interactions in full-length p53 in solution.
PMID:17612295
Yeast split-ubiquitin-based cytosolic screening system to detect interactions between transcriptionally active proteins.
PMID:17620598
Quaternary structures of tumor suppressor p53 and a specific p53 DNA complex.
PMID:18087040
Regulation of p53 tetramerization and nuclear export by ARC.
PMID:19339993
Crosstalk between sumoylation and acetylation regulates p53-dependent chromatin transcription and DNA binding.
PMID:19667193
Ultraslow oligomerization equilibria of p53 and its implications.
PMID:20004160
Averaging of electron subtomograms and random conical tilt reconstructions through likelihood optimization.
PMID:20159469
Crystal structure of the p53 core domain bound to a full consensus site as a self-assembled tetramer.
PMID:20364130
Apr 4. Diversity in DNA recognition by p53 revealed by crystal structures with Hoogsteen base pairs.
PMID:21178074
Electron microscopy studies on the quaternary structure of p53 reveal different binding modes for p53 tetramers in complex with DNA.
PMID:21522129
An induced fit mechanism regulates p53 DNA binding kinetics to confer sequence specificity.
PMID:21988832
Toward an understanding of the protein interaction network of the human liver.
PMID:22653443
Mutant p53 interactome identifies nardilysin as a p53R273H-specific binding partner that promotes invasion.
PMID:22972749
Domain-domain interactions in full-length p53 and a specific DNA complex probed by methyl NMR spectroscopy.
PMID:25402006
In silico prediction of physical protein interactions and characterization of interactome orphans.
PMID:25609649
Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes.
PMID:31837246
High-throughput competitive fluorescence polarization assay reveals functional redundancy in the S100 protein family.
PMID:35512704
2022 May 4. Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.
PMID:15629713
Binding of natively unfolded HIF-1alpha ODD domain to p53.
PMID:19011621
Arginine methylation regulates the p53 response.
GO:0042802 identical protein binding
IPI
PMID:31837246
High-throughput competitive fluorescence polarization assay ... 		ACCEPT
Summary: p53 binds to itself to form tetramers through its oligomerization domain, essential for DNA binding.
Reason: Core structural feature - p53 tetramerization through identical protein binding is essential for function.
Supporting Evidence:
PDB:1C26
Crystal structure of p53 tetramerization domain
PMID:10876243
Structure of the negative regulatory domain of p53 bound to S100B(betabeta).
PMID:14759370
Structural mechanism of the bromodomain of the coactivator CBP in p53 transcriptional activation.
PMID:14985081
Hepatitis C virus core protein interacts with p53-binding protein, 53BP2/Bbp/ASPP2, and inhibits p53-mediated apoptosis.
PMID:16291740
2005 Nov 15. Charge modification at multiple C-terminal lysine residues regulates p53 oligomerization and its nucleus-cytoplasm trafficking.
PMID:16461914
Core domain interactions in full-length p53 in solution.
PMID:17612295
Yeast split-ubiquitin-based cytosolic screening system to detect interactions between transcriptionally active proteins.
PMID:17620598
Quaternary structures of tumor suppressor p53 and a specific p53 DNA complex.
PMID:18087040
Regulation of p53 tetramerization and nuclear export by ARC.
PMID:19339993
Crosstalk between sumoylation and acetylation regulates p53-dependent chromatin transcription and DNA binding.
PMID:19667193
Ultraslow oligomerization equilibria of p53 and its implications.
PMID:20004160
Averaging of electron subtomograms and random conical tilt reconstructions through likelihood optimization.
PMID:20159469
Crystal structure of the p53 core domain bound to a full consensus site as a self-assembled tetramer.
PMID:20364130
Apr 4. Diversity in DNA recognition by p53 revealed by crystal structures with Hoogsteen base pairs.
PMID:21178074
Electron microscopy studies on the quaternary structure of p53 reveal different binding modes for p53 tetramers in complex with DNA.
PMID:21522129
An induced fit mechanism regulates p53 DNA binding kinetics to confer sequence specificity.
PMID:21988832
Toward an understanding of the protein interaction network of the human liver.
PMID:22653443
Mutant p53 interactome identifies nardilysin as a p53R273H-specific binding partner that promotes invasion.
PMID:22972749
Domain-domain interactions in full-length p53 and a specific DNA complex probed by methyl NMR spectroscopy.
PMID:25402006
In silico prediction of physical protein interactions and characterization of interactome orphans.
PMID:25609649
Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes.
PMID:31837246
High-throughput competitive fluorescence polarization assay reveals functional redundancy in the S100 protein family.
PMID:35512704
2022 May 4. Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.
PMID:15629713
Binding of natively unfolded HIF-1alpha ODD domain to p53.
PMID:19011621
Arginine methylation regulates the p53 response.
GO:0042802 identical protein binding
IPI
PMID:35512704
Systematic discovery of mutation-directed neo-protein-protei... 		ACCEPT
Summary: p53 binds to itself to form tetramers through its oligomerization domain, essential for DNA binding.
Reason: Core structural feature - p53 tetramerization through identical protein binding is essential for function.
Supporting Evidence:
PDB:1C26
Crystal structure of p53 tetramerization domain
PMID:10876243
Structure of the negative regulatory domain of p53 bound to S100B(betabeta).
PMID:14759370
Structural mechanism of the bromodomain of the coactivator CBP in p53 transcriptional activation.
PMID:14985081
Hepatitis C virus core protein interacts with p53-binding protein, 53BP2/Bbp/ASPP2, and inhibits p53-mediated apoptosis.
PMID:16291740
2005 Nov 15. Charge modification at multiple C-terminal lysine residues regulates p53 oligomerization and its nucleus-cytoplasm trafficking.
PMID:16461914
Core domain interactions in full-length p53 in solution.
PMID:17612295
Yeast split-ubiquitin-based cytosolic screening system to detect interactions between transcriptionally active proteins.
PMID:17620598
Quaternary structures of tumor suppressor p53 and a specific p53 DNA complex.
PMID:18087040
Regulation of p53 tetramerization and nuclear export by ARC.
PMID:19339993
Crosstalk between sumoylation and acetylation regulates p53-dependent chromatin transcription and DNA binding.
PMID:19667193
Ultraslow oligomerization equilibria of p53 and its implications.
PMID:20004160
Averaging of electron subtomograms and random conical tilt reconstructions through likelihood optimization.
PMID:20159469
Crystal structure of the p53 core domain bound to a full consensus site as a self-assembled tetramer.
PMID:20364130
Apr 4. Diversity in DNA recognition by p53 revealed by crystal structures with Hoogsteen base pairs.
PMID:21178074
Electron microscopy studies on the quaternary structure of p53 reveal different binding modes for p53 tetramers in complex with DNA.
PMID:21522129
An induced fit mechanism regulates p53 DNA binding kinetics to confer sequence specificity.
PMID:21988832
Toward an understanding of the protein interaction network of the human liver.
PMID:22653443
Mutant p53 interactome identifies nardilysin as a p53R273H-specific binding partner that promotes invasion.
PMID:22972749
Domain-domain interactions in full-length p53 and a specific DNA complex probed by methyl NMR spectroscopy.
PMID:25402006
In silico prediction of physical protein interactions and characterization of interactome orphans.
PMID:25609649
Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes.
PMID:31837246
High-throughput competitive fluorescence polarization assay reveals functional redundancy in the S100 protein family.
PMID:35512704
2022 May 4. Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.
PMID:15629713
Binding of natively unfolded HIF-1alpha ODD domain to p53.
PMID:19011621
Arginine methylation regulates the p53 response.
GO:0017053 transcription repressor complex
IPI
PMID:18677113
Structure of the human Mdmx protein bound to the p53 tumor s... 		ACCEPT
Summary: p53 functions within transcriptional regulator complexes to control gene expression.
Reason: Core function - p53 forms complexes with transcriptional machinery and co-factors.
Supporting Evidence:
TP53-deep-research
Complex formation with transcriptional co-activators (CBP/p300, TFIID)
PMID:18677113
Structure of the human Mdmx protein bound to the p53 tumor suppressor transactivation domain.
PMID:8875929
Structure of the MDM2 oncoprotein bound to the p53 tumor suppressor transactivation domain.
GO:0017053 transcription repressor complex
IPI
PMID:8875929
Structure of the MDM2 oncoprotein bound to the p53 tumor sup... 		ACCEPT
Summary: p53 functions within transcriptional regulator complexes to control gene expression.
Reason: Core function - p53 forms complexes with transcriptional machinery and co-factors.
Supporting Evidence:
TP53-deep-research
Complex formation with transcriptional co-activators (CBP/p300, TFIID)
PMID:18677113
Structure of the human Mdmx protein bound to the p53 tumor suppressor transactivation domain.
PMID:8875929
Structure of the MDM2 oncoprotein bound to the p53 tumor suppressor transactivation domain.
GO:0045892 negative regulation of DNA-templated transcription
NAS
PMID:16492744
Mdm4 and Mdm2 cooperate to inhibit p53 activity in prolifera... 		ACCEPT
Summary: p53 negatively regulates transcription of various target genes including anti-apoptotic and cell cycle genes.
Reason: Core function - p53 acts as both a transcriptional activator and repressor.
Supporting Evidence:
TP53-deep-research
Repression: Downregulates anti-apoptotic genes (BCL2), cell cycle progression genes
PMID:16492744
Mdm4 and Mdm2 cooperate to inhibit p53 activity in proliferating and quiescent cells in vivo.
PMID:9271120
Repression of p53-mediated transcription by MDM2: a dual mechanism.
PMID:23629966
Deacetylation of p53 induces autophagy by suppressing Bmf expression.
PMID:24051492
p53 regulates Period2 expression and the circadian clock.
GO:0045892 negative regulation of DNA-templated transcription
IDA
PMID:9271120
Repression of p53-mediated transcription by MDM2: a dual mec... 		ACCEPT
Summary: p53 negatively regulates transcription of various target genes including anti-apoptotic and cell cycle genes.
Reason: Core function - p53 acts as both a transcriptional activator and repressor.
Supporting Evidence:
TP53-deep-research
Repression: Downregulates anti-apoptotic genes (BCL2), cell cycle progression genes
PMID:16492744
Mdm4 and Mdm2 cooperate to inhibit p53 activity in proliferating and quiescent cells in vivo.
PMID:9271120
Repression of p53-mediated transcription by MDM2: a dual mechanism.
PMID:23629966
Deacetylation of p53 induces autophagy by suppressing Bmf expression.
PMID:24051492
p53 regulates Period2 expression and the circadian clock.
GO:0000977 RNA polymerase II transcription regulatory region sequence-specific DNA binding
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 binds to RNA polymerase II cis-regulatory regions in target gene promoters.
Reason: Core molecular function - sequence-specific DNA binding is essential for p53 transcription factor activity.
Supporting Evidence:
TP53-deep-research
TP53 is fundamentally a transcription factor that binds sequence-specifically to p53 response elements
GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 binds sequence-specifically to p53 response elements in cis-regulatory regions of target genes.
Reason: Core molecular function - p53's DNA-binding domain binds to consensus p53 response elements (p53REs) with high specificity.
Supporting Evidence:
TP53-deep-research
TP53 is fundamentally a transcription factor that binds sequence-specifically to p53 response elements (p53REs) in target gene promoters
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:22578566
Apr 19. Oncosuppressive role of p53-induced miR-205 in triple negative breast cancer.
GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 activates transcription through binding to promoter sequences.
Reason: Core molecular function - p53 is a transcriptional activator that binds to p53 response elements.
Supporting Evidence:
TP53-deep-research
TP53 is fundamentally a transcription factor that binds sequence-specifically to p53 response elements
PMID:22578566
Apr 19. Oncosuppressive role of p53-induced miR-205 in triple negative breast cancer.
PMID:17145718
Brn-3b enhances the pro-apoptotic effects of p53 but not its induction of cell cycle arrest by cooperating in trans-activation of bax expression.
PMID:12609999
2003 Feb 27. The activation domains, the proline-rich domain, and the C-terminal basic domain in p53 are necessary for acetylation of histones on the proximal p21 promoter and interaction with p300/CREB-binding protein.
PMID:17146433
Dec 4. RGC32, a novel p53-inducible gene, is located on centrosomes during mitosis and results in G2/M arrest.
GO:0003682 chromatin binding
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 binds chromatin at p53 response elements in target gene promoters.
Reason: Core molecular function - chromatin binding is essential for p53's transcriptional regulation.
Supporting Evidence:
PDB:3TS8
Crystal structure of p53 tetramer bound to DNA
PMID:26334721
2015 Sep 3. Jmjd5 functions as a regulator of p53 signaling during mouse embryogenesis.
PMID:16322561
NIR is a novel INHAT repressor that modulates the transcriptional activity of p53.
PMID:17599062
Jun 28. CDIP, a novel pro-apoptotic gene, regulates TNFalpha-mediated apoptosis in a p53-dependent manner.
GO:0005657 replication fork
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 is a component of replication fork DNA repair machinery.
Reason: p53 coordinates DNA repair at replication forks during DNA damage response.
GO:0005739 mitochondrion
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 translocates to mitochondria to directly promote apoptosis through BAX activation.
Reason: Important localization for non-transcriptional apoptosis - p53 can directly induce MOMP at mitochondria.
Supporting Evidence:
TP53-deep-research
Non-transcriptional: Direct mitochondrial translocation and interaction with BCL-2 family proteins
PMID:25168243
Aug 29. Cofactor Strap regulates oxidative phosphorylation and mitochondrial p53 activity through ATP synthase.
PMID:24101517
Mitochondrial disulfide relay mediates translocation of p53 and partitions its subcellular activity.
PMID:12667443
p53 has a direct apoptogenic role at the mitochondria.
GO:0005829 cytosol
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 localizes to cytosol as part of its nucleo-cytoplasmic shuttling.
Reason: p53 shuttles between nucleus and cytoplasm, with cytosolic localization important for regulation.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus. Cytoplasm
PMID:24101517
Mitochondrial disulfide relay mediates translocation of p53 and partitions its subcellular activity.
PMID:14963330
Direct activation of Bax by p53 mediates mitochondrial membrane permeabilization and apoptosis.
GO:0007623 circadian rhythm
IEA
GO_REF:0000107 		KEEP AS NON CORE
Summary: p53 may influence circadian rhythms but this is not a primary function.
Reason: Non-core function - p53 has connections to circadian biology.
GO:0008285 negative regulation of cell population proliferation
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 negatively regulates cell proliferation as a fundamental tumor suppressor mechanism.
Reason: Core function - p53 inhibits proliferation through cell cycle arrest.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Negatively regulates cell division
PMID:30514107
CELF1/p53 axis: a sustained antiproliferative signal leading to villus atrophy under total parenteral nutrition.
PMID:12730672
Physical and functional interaction between HCV core protein and the different p73 isoforms.
PMID:22783376
Induction of apoptosis by cytoplasmically localized wild-type p53 and the S121F mutant super p53.
GO:0008340 determination of adult lifespan
IEA
GO_REF:0000107 		KEEP AS NON CORE
Summary: p53 may influence lifespan through its regulation of senescence and apoptosis.
Reason: Non-core function - while p53 influences aging, this is secondary to its tumor suppressor function.
GO:0031625 ubiquitin protein ligase binding
IEA
GO_REF:0000120 		REMOVE
Summary: Generic 'ubiquitin protein ligase binding' is less informative than specific MDM2/MDM4 interactions.
Reason: While p53 binds MDM2 (E3 ligase), this generic term is uninformative. The specific MDM2 interaction is well-documented elsewhere.
GO:0034644 cellular response to UV
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 responds to UV-induced DNA damage as part of its DNA damage response.
Reason: Core function - UV radiation is a classic p53-activating stress.
Supporting Evidence:
GO:0009411
response to UV
PMID:16213212
Regulation of p53 translation and induction after DNA damage by ribosomal protein L26 and nucleolin.
GO:0035033 histone deacetylase regulator activity
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 undergoes deacetylation by histone deacetylases including HDAC1.
Reason: Core regulatory mechanism - p53 deacetylation by HDACs regulates its activity.
Supporting Evidence:
TP53-deep-research
Acetylation/deacetylation regulates p53 function
GO:0035861 site of double-strand break
IEA
GO_REF:0000107 		KEEP AS NON CORE
Summary: p53 is involved in ventral column development in spinal cord.
Reason: Non-core tissue-specific developmental function.
GO:0042771 intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 is THE p53 class mediator that induces intrinsic apoptosis in response to DNA damage through BAX, PUMA, NOXA activation.
Reason: Absolutely core function - this term specifically describes p53's role as the central mediator of DNA damage-induced apoptosis.
Supporting Evidence:
TP53-deep-research
Transcriptional: Activation of pro-apoptotic genes (PUMA, BAX, NOXA, FAS)
PMID:14654789
A member of the Pyrin family, IFI16, is a novel BRCA1-associated protein involved in the p53-mediated apoptosis pathway.
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:16213212
Regulation of p53 translation and induction after DNA damage by ribosomal protein L26 and nucleolin.
PMID:17403783
HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53.
GO:0043073 germ cell nucleus
IEA
GO_REF:0000107 		KEEP AS NON CORE
Summary: p53 is involved in germ cell development but is not essential.
Reason: Non-core developmental function.
GO:0043153 entrainment of circadian clock by photoperiod
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 enters the nucleus to function as a transcription factor.
Reason: Core process - nuclear entry is essential for p53's transcription factor activity.
Supporting Evidence:
TP53-deep-research
Nuclear Localization Signals (NLS): Function: Nuclear import and localization
GO:0045892 negative regulation of DNA-templated transcription
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 negatively regulates transcription of various target genes including anti-apoptotic and cell cycle genes.
Reason: Core function - p53 acts as both a transcriptional activator and repressor.
Supporting Evidence:
TP53-deep-research
Repression: Downregulates anti-apoptotic genes (BCL2), cell cycle progression genes
PMID:16492744
Mdm4 and Mdm2 cooperate to inhibit p53 activity in proliferating and quiescent cells in vivo.
PMID:9271120
Repression of p53-mediated transcription by MDM2: a dual mechanism.
PMID:23629966
Deacetylation of p53 induces autophagy by suppressing Bmf expression.
PMID:24051492
p53 regulates Period2 expression and the circadian clock.
GO:0048512 circadian behavior
IEA
GO_REF:0000107 		KEEP AS NON CORE
Summary: p53 is involved in organ development but is not essential for development.
Reason: Non-core developmental function - p53 knockout mice develop normally.
GO:0060333 type II interferon-mediated signaling pathway
IEA
GO_REF:0000107 		KEEP AS NON CORE
Summary: p53 may influence interferon-gamma signaling but this is not a primary function.
Reason: Non-core immunomodulatory function.
GO:0062100 positive regulation of programmed necrotic cell death
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 regulates intrinsic apoptotic signaling in response to various DNA damage types.
Reason: Core function - p53 responds to diverse DNA damage to trigger apoptosis.
GO:0070059 intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 is phosphorylated on serine residues including Ser15, Ser20, Ser46, Ser392.
Reason: Core regulatory mechanism - serine phosphorylation is essential for p53 activation.
Supporting Evidence:
TP53-deep-research
Ser15, Thr18, Ser20: N-terminal phosphorylation by ATM/ATR, disrupts MDM2 binding
GO:0070245 positive regulation of thymocyte apoptotic process
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 responds to positive regulation by stress-induced post-translational modifications.
Reason: p53 is regulated by extensive post-translational modifications that enhance its activity.
Supporting Evidence:
TP53-deep-research
p53 can be modified at over 60 of its 393 residues
GO:0071479 cellular response to ionizing radiation
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 responds to UV-A radiation-induced DNA damage.
Reason: Core DNA damage response function - UV radiation is a classic p53 activator.
Supporting Evidence:
GO:0009411
response to UV
PMID:20160708
Feedback between p21 and reactive oxygen production is necessary for cell senescence.
GO:0097371 MDM2/MDM4 family protein binding
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 undergoes mitochondrial translocation to directly promote apoptosis.
Reason: Important function - p53 can induce apoptosis through non-transcriptional mitochondrial mechanisms.
Supporting Evidence:
TP53-deep-research
Direct mitochondrial translocation and interaction with BCL-2 family proteins
GO:2000774 positive regulation of cellular senescence
IEA
GO_REF:0000107 		ACCEPT
Summary: p53 positively regulates cellular senescence as an alternative to apoptosis.
Reason: Core function - senescence induction is a major tumor suppressor mechanism of p53.
Supporting Evidence:
TP53-deep-research
p53 induces senescence through p21 and other targets as an irreversible cell cycle arrest mechanism
PMID:29474172
Haploinsufficiency of Trp53 dramatically extends the lifespan of Sirt6-deficient mice.
GO:0005654 nucleoplasm
IDA
PMID:12915590
Cellular stress and DNA damage invoke temporally distinct Md... 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0072331 signal transduction by p53 class mediator
IDA
PMID:25384516
Rad54B serves as a scaffold in the DNA damage response that ... 		ACCEPT
Summary: p53 IS the p53 class mediator that transduces signals in response to stress.
Reason: Absolutely core function - this term specifically describes p53's central role in stress signaling.
Supporting Evidence:
TP53-deep-research
p53 coordinates cellular responses to diverse stress signals
PMID:25384516
Rad54B serves as a scaffold in the DNA damage response that limits checkpoint strength.
PMID:18549481
TATA binding protein associated factor 3 (TAF3) interacts with p53 and inhibits its function.
PMID:29681526
A Designed Peptide Targets Two Types of Modifications of p53 with Anti-cancer Activity.
PMID:15314173
Ribosomal protein L23 activates p53 by inhibiting MDM2 function in response to ribosomal perturbation but not to translation inhibition.
GO:1990841 promoter-specific chromatin binding
IDA
PMID:24652652
DRAGO (KIAA0247), a new DNA damage-responsive, p53-inducible... 		ACCEPT
Summary: p53 binds to promoter-specific chromatin regions containing p53 response elements.
Reason: Core molecular function - p53 specifically binds to promoter regions of target genes through sequence-specific DNA binding.
Supporting Evidence:
PDB:3TS8
Crystal structure shows p53 binding to CDKN1A(p21) promoter response element
PMID:24652652
2014 Mar 20. DRAGO (KIAA0247), a new DNA damage-responsive, p53-inducible gene that cooperates with p53 as oncosuppressor.
PMID:20725088
2010 Aug 20. Primate-specific RFPL1 gene controls cell-cycle progression through cyclin B1/Cdc2 degradation.
PMID:24356969
2013 Dec 19. Rbm24, an RNA-binding protein and a target of p53, regulates p21 expression via mRNA stability.
GO:0072331 signal transduction by p53 class mediator
IDA
PMID:18549481
TATA binding protein associated factor 3 (TAF3) interacts wi... 		ACCEPT
Summary: p53 IS the p53 class mediator that transduces signals in response to stress.
Reason: Absolutely core function - this term specifically describes p53's central role in stress signaling.
Supporting Evidence:
TP53-deep-research
p53 coordinates cellular responses to diverse stress signals
PMID:25384516
Rad54B serves as a scaffold in the DNA damage response that limits checkpoint strength.
PMID:18549481
TATA binding protein associated factor 3 (TAF3) interacts with p53 and inhibits its function.
PMID:29681526
A Designed Peptide Targets Two Types of Modifications of p53 with Anti-cancer Activity.
PMID:15314173
Ribosomal protein L23 activates p53 by inhibiting MDM2 function in response to ribosomal perturbation but not to translation inhibition.
GO:0000785 chromatin
IDA
PMID:24289924
Phosphorylation of p53 by TAF1 inactivates p53-dependent tra... 		ACCEPT
Summary: p53 associates with chromatin at target gene promoters to regulate transcription.
Reason: Core molecular function - p53 binds to chromatin at p53 response elements to regulate target gene expression.
Supporting Evidence:
PDB:3TS8
Crystal structure of a multidomain human p53 tetramer bound to the natural CDKN1A(p21) p53-response element
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:30089260
p53 Regulates the Expression of LRP1 and Apoptosis through a Stress Intensity-Dependent MicroRNA Feedback Loop.
PMID:23629966
Deacetylation of p53 induces autophagy by suppressing Bmf expression.
PMID:22521434
Hepatocyte growth factor (HGF) and 1,25-dihydroxyvitamin D together stimulate human bone marrow-derived stem cells toward the osteogenic phenotype by HGF-induced up-regulation of VDR.
PMID:15710329
Human MUC1 oncoprotein regulates p53-responsive gene transcription in the genotoxic stress response.
PMID:17805299
p53 is regulated by the lysine demethylase LSD1.
GO:0003700 DNA-binding transcription factor activity
IMP
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negat... 		ACCEPT
Summary: p53 is a well-characterized DNA-binding transcription factor that activates and represses target genes.
Reason: Core molecular function - p53 is one of the best-characterized transcription factors, regulating >500 target genes.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:26334721
2015 Sep 3. Jmjd5 functions as a regulator of p53 signaling during mouse embryogenesis.
PMID:18549481
TATA binding protein associated factor 3 (TAF3) interacts with p53 and inhibits its function.
PMID:7587074
Targets for transcriptional activation by wild-type p53: endogenous retroviral LTR, immunoglobulin-like promoter, and an internal promoter of the mdm2 gene.
GO:0030330 DNA damage response, signal transduction by p53 class mediator
IDA
PMID:29681526
A Designed Peptide Targets Two Types of Modifications of p53... 		ACCEPT
Summary: p53 mediates DNA damage response signal transduction as THE p53 class mediator.
Reason: Absolutely core function - p53 is the defining member of the p53 class mediator family.
Supporting Evidence:
TP53-deep-research
p53 serves as a central node in the DNA damage response network
PMID:29681526
A Designed Peptide Targets Two Types of Modifications of p53 with Anti-cancer Activity.
PMID:15149599
Telomere shortening triggers senescence of human cells through a pathway involving ATM, p53, and p21(CIP1), but not p16(INK4a).
PMID:20160708
Feedback between p21 and reactive oxygen production is necessary for cell senescence.
PMID:7958916
DNA damage triggers a prolonged p53-dependent G1 arrest and long-term induction of Cip1 in normal human fibroblasts.
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:16213212
Regulation of p53 translation and induction after DNA damage by ribosomal protein L26 and nucleolin.
GO:0010628 positive regulation of gene expression
ISS
GO_REF:0000024 		ACCEPT
Summary: p53 positively regulates gene expression of numerous target genes.
Reason: Core transcriptional function - p53 is a transcriptional activator.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:26100857
A metabolic stress-inducible miR-34a-HNF4Ξ± pathway regulates lipid and lipoprotein metabolism.
PMID:20332243
2010 Mar 23. MicroRNA145 targets BNIP3 and suppresses prostate cancer progression.
PMID:15314173
Ribosomal protein L23 activates p53 by inhibiting MDM2 function in response to ribosomal perturbation but not to translation inhibition.
PMID:19160485
A ribosomal protein L23-nucleophosmin circuit coordinates Mizl function with cell growth.
GO:0001223 transcription coactivator binding
IPI
PMID:11950834
SWI/SNF complex interacts with tumor suppressor p53 and is n... 		ACCEPT
Summary: p53 functions as a transcription activator at p53 response elements.
Reason: Core molecular function - p53 activates transcription at cis-regulatory elements.
Supporting Evidence:
TP53-deep-research
p53 binds sequence-specifically to p53 response elements
PMID:11950834
2002 Apr 11. SWI/SNF complex interacts with tumor suppressor p53 and is necessary for the activation of p53-mediated transcription.
GO:0030330 DNA damage response, signal transduction by p53 class mediator
IDA
PMID:15149599
Telomere shortening triggers senescence of human cells throu... 		ACCEPT
Summary: p53 mediates DNA damage response signal transduction as THE p53 class mediator.
Reason: Absolutely core function - p53 is the defining member of the p53 class mediator family.
Supporting Evidence:
TP53-deep-research
p53 serves as a central node in the DNA damage response network
PMID:29681526
A Designed Peptide Targets Two Types of Modifications of p53 with Anti-cancer Activity.
PMID:15149599
Telomere shortening triggers senescence of human cells through a pathway involving ATM, p53, and p21(CIP1), but not p16(INK4a).
PMID:20160708
Feedback between p21 and reactive oxygen production is necessary for cell senescence.
PMID:7958916
DNA damage triggers a prolonged p53-dependent G1 arrest and long-term induction of Cip1 in normal human fibroblasts.
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:16213212
Regulation of p53 translation and induction after DNA damage by ribosomal protein L26 and nucleolin.
GO:0005654 nucleoplasm
IDA
GO_REF:0000052 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005829 cytosol
IDA
GO_REF:0000052 		ACCEPT
Summary: p53 localizes to cytosol as part of its nucleo-cytoplasmic shuttling.
Reason: p53 shuttles between nucleus and cytoplasm, with cytosolic localization important for regulation.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus. Cytoplasm
PMID:24101517
Mitochondrial disulfide relay mediates translocation of p53 and partitions its subcellular activity.
PMID:14963330
Direct activation of Bax by p53 mediates mitochondrial membrane permeabilization and apoptosis.
GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific
IDA
PMID:34381247
The tumor suppressor folliculin inhibits lactate dehydrogena... 		ACCEPT
Summary: p53 is a DNA-binding transcription factor that regulates RNA polymerase II transcription of target genes.
Reason: Core molecular function - p53 is a well-characterized sequence-specific transcription factor for RNA pol II.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:34381247
Epub 2021 Aug 11. The tumor suppressor folliculin inhibits lactate dehydrogenase A and regulates the Warburg effect.
PMID:35618207
Ser392 phosphorylation modulated a switch between p53 and transcriptional condensates.
PMID:36634798
Phosphorylation and specific DNA improved the incorporation ability of p53 into functional condensates.
PMID:38653238
2024 Apr 22. Alanyl-tRNA synthetase, AARS1, is a lactate sensor and lactyltransferase that lactylates p53 and contributes to tumorigenesis.
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:17310983
Feb 19. Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function.
PMID:24652652
2014 Mar 20. DRAGO (KIAA0247), a new DNA damage-responsive, p53-inducible gene that cooperates with p53 as oncosuppressor.
GO:0005634 nucleus
IDA
PMID:14963330
Direct activation of Bax by p53 mediates mitochondrial membr... 		ACCEPT
Summary: p53 is primarily a nuclear transcription factor, with nuclear localization essential for its function.
Reason: Core cellular component - p53's nuclear localization is essential for its transcription factor activity. Contains nuclear localization signals at residues 305-322 and 369-375.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Endoplasmic reticulum.
PMID:14963330
Direct activation of Bax by p53 mediates mitochondrial membrane permeabilization and apoptosis.
PMID:16322561
NIR is a novel INHAT repressor that modulates the transcriptional activity of p53.
PMID:36108750
Phase separation of p53 induced by its unstructured basic region and prevented by oncogenic mutations in tetramerization domain.
PMID:24625977
Ubiquitin-like (UBX)-domain-containing protein, UBXN2A, promotes cell death by interfering with the p53-Mortalin interactions in colon cancer cells.
PMID:26634371
Epub 2015 Dec 4. Structural studies of UBXN2A and mortalin interaction and the putative role of silenced UBXN2A in preventing response to chemotherapy.
PMID:21597459
2011 May 20. E3 ubiquitin ligase Hades negatively regulates the exonuclear function of p53.
PMID:19011621
Arginine methylation regulates the p53 response.
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:24101517
Mitochondrial disulfide relay mediates translocation of p53 and partitions its subcellular activity.
PMID:20810912
Hypoxia downregulates p53 but induces apoptosis and enhances expression of BAD in cultures of human syncytiotrophoblasts.
PMID:19234109
Induction of SOX4 by DNA damage is critical for p53 stabilization and function.
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:17403783
HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53.
PMID:20096447
USP10 regulates p53 localization and stability by deubiquitinating p53.
PMID:18756595
Prognostic significance of BMP and activin membrane-bound inhibitor in colorectal cancer.
PMID:16507995
Myosin VI is a mediator of the p53-dependent cell survival pathway.
PMID:7720704
Direct involvement of p53 in programmed cell death of oligodendrocytes.
GO:0005634 nucleus
IDA
PMID:16322561
NIR is a novel INHAT repressor that modulates the transcript... 		ACCEPT
Summary: p53 is primarily a nuclear transcription factor, with nuclear localization essential for its function.
Reason: Core cellular component - p53's nuclear localization is essential for its transcription factor activity. Contains nuclear localization signals at residues 305-322 and 369-375.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Endoplasmic reticulum.
PMID:14963330
Direct activation of Bax by p53 mediates mitochondrial membrane permeabilization and apoptosis.
PMID:16322561
NIR is a novel INHAT repressor that modulates the transcriptional activity of p53.
PMID:36108750
Phase separation of p53 induced by its unstructured basic region and prevented by oncogenic mutations in tetramerization domain.
PMID:24625977
Ubiquitin-like (UBX)-domain-containing protein, UBXN2A, promotes cell death by interfering with the p53-Mortalin interactions in colon cancer cells.
PMID:26634371
Epub 2015 Dec 4. Structural studies of UBXN2A and mortalin interaction and the putative role of silenced UBXN2A in preventing response to chemotherapy.
PMID:21597459
2011 May 20. E3 ubiquitin ligase Hades negatively regulates the exonuclear function of p53.
PMID:19011621
Arginine methylation regulates the p53 response.
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:24101517
Mitochondrial disulfide relay mediates translocation of p53 and partitions its subcellular activity.
PMID:20810912
Hypoxia downregulates p53 but induces apoptosis and enhances expression of BAD in cultures of human syncytiotrophoblasts.
PMID:19234109
Induction of SOX4 by DNA damage is critical for p53 stabilization and function.
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:17403783
HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53.
PMID:20096447
USP10 regulates p53 localization and stability by deubiquitinating p53.
PMID:18756595
Prognostic significance of BMP and activin membrane-bound inhibitor in colorectal cancer.
PMID:16507995
Myosin VI is a mediator of the p53-dependent cell survival pathway.
PMID:7720704
Direct involvement of p53 in programmed cell death of oligodendrocytes.
GO:0030330 DNA damage response, signal transduction by p53 class mediator
IMP
PMID:20160708
Feedback between p21 and reactive oxygen production is neces... 		ACCEPT
Summary: p53 mediates DNA damage response signal transduction as THE p53 class mediator.
Reason: Absolutely core function - p53 is the defining member of the p53 class mediator family.
Supporting Evidence:
TP53-deep-research
p53 serves as a central node in the DNA damage response network
PMID:29681526
A Designed Peptide Targets Two Types of Modifications of p53 with Anti-cancer Activity.
PMID:15149599
Telomere shortening triggers senescence of human cells through a pathway involving ATM, p53, and p21(CIP1), but not p16(INK4a).
PMID:20160708
Feedback between p21 and reactive oxygen production is necessary for cell senescence.
PMID:7958916
DNA damage triggers a prolonged p53-dependent G1 arrest and long-term induction of Cip1 in normal human fibroblasts.
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:16213212
Regulation of p53 translation and induction after DNA damage by ribosomal protein L26 and nucleolin.
GO:0140693 molecular condensate scaffold activity
IDA
PMID:38653238
Alanyl-tRNA synthetase, AARS1, is a lactate sensor and lacty... 		ACCEPT
Summary: p53 binds to single-stranded DNA through its DNA-binding domain.
Reason: p53 can bind single-stranded DNA as part of its DNA repair and damage sensing functions.
Supporting Evidence:
PMID:38653238
2024 Apr 22. Alanyl-tRNA synthetase, AARS1, is a lactate sensor and lactyltransferase that lactylates p53 and contributes to tumorigenesis.
GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific
IDA
PMID:35618207
Ser392 phosphorylation modulated a switch between p53 and tr... 		ACCEPT
Summary: p53 is a DNA-binding transcription factor that regulates RNA polymerase II transcription of target genes.
Reason: Core molecular function - p53 is a well-characterized sequence-specific transcription factor for RNA pol II.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:34381247
Epub 2021 Aug 11. The tumor suppressor folliculin inhibits lactate dehydrogenase A and regulates the Warburg effect.
PMID:35618207
Ser392 phosphorylation modulated a switch between p53 and transcriptional condensates.
PMID:36634798
Phosphorylation and specific DNA improved the incorporation ability of p53 into functional condensates.
PMID:38653238
2024 Apr 22. Alanyl-tRNA synthetase, AARS1, is a lactate sensor and lactyltransferase that lactylates p53 and contributes to tumorigenesis.
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:17310983
Feb 19. Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function.
PMID:24652652
2014 Mar 20. DRAGO (KIAA0247), a new DNA damage-responsive, p53-inducible gene that cooperates with p53 as oncosuppressor.
GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific
IDA
PMID:36634798
Phosphorylation and specific DNA improved the incorporation ... 		ACCEPT
Summary: p53 is a DNA-binding transcription factor that regulates RNA polymerase II transcription of target genes.
Reason: Core molecular function - p53 is a well-characterized sequence-specific transcription factor for RNA pol II.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:34381247
Epub 2021 Aug 11. The tumor suppressor folliculin inhibits lactate dehydrogenase A and regulates the Warburg effect.
PMID:35618207
Ser392 phosphorylation modulated a switch between p53 and transcriptional condensates.
PMID:36634798
Phosphorylation and specific DNA improved the incorporation ability of p53 into functional condensates.
PMID:38653238
2024 Apr 22. Alanyl-tRNA synthetase, AARS1, is a lactate sensor and lactyltransferase that lactylates p53 and contributes to tumorigenesis.
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:17310983
Feb 19. Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function.
PMID:24652652
2014 Mar 20. DRAGO (KIAA0247), a new DNA damage-responsive, p53-inducible gene that cooperates with p53 as oncosuppressor.
GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific
IDA
PMID:38653238
Alanyl-tRNA synthetase, AARS1, is a lactate sensor and lacty... 		ACCEPT
Summary: p53 is a DNA-binding transcription factor that regulates RNA polymerase II transcription of target genes.
Reason: Core molecular function - p53 is a well-characterized sequence-specific transcription factor for RNA pol II.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:34381247
Epub 2021 Aug 11. The tumor suppressor folliculin inhibits lactate dehydrogenase A and regulates the Warburg effect.
PMID:35618207
Ser392 phosphorylation modulated a switch between p53 and transcriptional condensates.
PMID:36634798
Phosphorylation and specific DNA improved the incorporation ability of p53 into functional condensates.
PMID:38653238
2024 Apr 22. Alanyl-tRNA synthetase, AARS1, is a lactate sensor and lactyltransferase that lactylates p53 and contributes to tumorigenesis.
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:17310983
Feb 19. Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function.
PMID:24652652
2014 Mar 20. DRAGO (KIAA0247), a new DNA damage-responsive, p53-inducible gene that cooperates with p53 as oncosuppressor.
GO:0005634 nucleus
IDA
PMID:36108750
Phase separation of p53 induced by its unstructured basic re... 		ACCEPT
Summary: p53 is primarily a nuclear transcription factor, with nuclear localization essential for its function.
Reason: Core cellular component - p53's nuclear localization is essential for its transcription factor activity. Contains nuclear localization signals at residues 305-322 and 369-375.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Endoplasmic reticulum.
PMID:14963330
Direct activation of Bax by p53 mediates mitochondrial membrane permeabilization and apoptosis.
PMID:16322561
NIR is a novel INHAT repressor that modulates the transcriptional activity of p53.
PMID:36108750
Phase separation of p53 induced by its unstructured basic region and prevented by oncogenic mutations in tetramerization domain.
PMID:24625977
Ubiquitin-like (UBX)-domain-containing protein, UBXN2A, promotes cell death by interfering with the p53-Mortalin interactions in colon cancer cells.
PMID:26634371
Epub 2015 Dec 4. Structural studies of UBXN2A and mortalin interaction and the putative role of silenced UBXN2A in preventing response to chemotherapy.
PMID:21597459
2011 May 20. E3 ubiquitin ligase Hades negatively regulates the exonuclear function of p53.
PMID:19011621
Arginine methylation regulates the p53 response.
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:24101517
Mitochondrial disulfide relay mediates translocation of p53 and partitions its subcellular activity.
PMID:20810912
Hypoxia downregulates p53 but induces apoptosis and enhances expression of BAD in cultures of human syncytiotrophoblasts.
PMID:19234109
Induction of SOX4 by DNA damage is critical for p53 stabilization and function.
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:17403783
HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53.
PMID:20096447
USP10 regulates p53 localization and stability by deubiquitinating p53.
PMID:18756595
Prognostic significance of BMP and activin membrane-bound inhibitor in colorectal cancer.
PMID:16507995
Myosin VI is a mediator of the p53-dependent cell survival pathway.
PMID:7720704
Direct involvement of p53 in programmed cell death of oligodendrocytes.
GO:0140693 molecular condensate scaffold activity
IDA
PMID:31953488
Liquid-like droplet formation by tumor suppressor p53 induce... 		ACCEPT
Summary: p53 binds to single-stranded DNA through its DNA-binding domain.
Reason: p53 can bind single-stranded DNA as part of its DNA repair and damage sensing functions.
Supporting Evidence:
PMID:31953488
Liquid-like droplet formation by tumor suppressor p53 induced by multivalent electrostatic interactions between two disordered domains.
GO:0140693 molecular condensate scaffold activity
IDA
PMID:35618207
Ser392 phosphorylation modulated a switch between p53 and tr... 		ACCEPT
Summary: p53 binds to single-stranded DNA through its DNA-binding domain.
Reason: p53 can bind single-stranded DNA as part of its DNA repair and damage sensing functions.
Supporting Evidence:
PMID:35618207
Ser392 phosphorylation modulated a switch between p53 and transcriptional condensates.
GO:0140693 molecular condensate scaffold activity
IDA
PMID:36108750
Phase separation of p53 induced by its unstructured basic re... 		ACCEPT
Summary: p53 binds to single-stranded DNA through its DNA-binding domain.
Reason: p53 can bind single-stranded DNA as part of its DNA repair and damage sensing functions.
Supporting Evidence:
PMID:36108750
Phase separation of p53 induced by its unstructured basic region and prevented by oncogenic mutations in tetramerization domain.
GO:0140693 molecular condensate scaffold activity
IDA
PMID:36634798
Phosphorylation and specific DNA improved the incorporation ... 		ACCEPT
Summary: p53 binds to single-stranded DNA through its DNA-binding domain.
Reason: p53 can bind single-stranded DNA as part of its DNA repair and damage sensing functions.
Supporting Evidence:
PMID:36634798
Phosphorylation and specific DNA improved the incorporation ability of p53 into functional condensates.
GO:0030330 DNA damage response, signal transduction by p53 class mediator
IMP
PMID:7958916
DNA damage triggers a prolonged p53-dependent G1 arrest and ... 		ACCEPT
Summary: p53 mediates DNA damage response signal transduction as THE p53 class mediator.
Reason: Absolutely core function - p53 is the defining member of the p53 class mediator family.
Supporting Evidence:
TP53-deep-research
p53 serves as a central node in the DNA damage response network
PMID:29681526
A Designed Peptide Targets Two Types of Modifications of p53 with Anti-cancer Activity.
PMID:15149599
Telomere shortening triggers senescence of human cells through a pathway involving ATM, p53, and p21(CIP1), but not p16(INK4a).
PMID:20160708
Feedback between p21 and reactive oxygen production is necessary for cell senescence.
PMID:7958916
DNA damage triggers a prolonged p53-dependent G1 arrest and long-term induction of Cip1 in normal human fibroblasts.
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:16213212
Regulation of p53 translation and induction after DNA damage by ribosomal protein L26 and nucleolin.
GO:0032211 negative regulation of telomere maintenance via telomerase
IDA
PMID:10597287
Molecular interactions between telomerase and the tumor supp... 		ACCEPT
Summary: p53 negatively regulates telomerase activity.
Reason: p53 suppresses telomerase as part of its tumor suppressor function.
Supporting Evidence:
PMID:10597287
Molecular interactions between telomerase and the tumor suppressor protein p53 in vitro.
GO:0045944 positive regulation of transcription by RNA polymerase II
ISS
GO_REF:0000024 		ACCEPT
Summary: p53 is a sequence-specific transcriptional activator that upregulates target genes including p21, PUMA, BAX.
Reason: Fundamental molecular function - p53 activates transcription of numerous target genes through binding to p53 response elements.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:16061649
Ckap2 regulates aneuploidy, cell cycling, and cell death in a p53-dependent manner.
PMID:17599062
Jun 28. CDIP, a novel pro-apoptotic gene, regulates TNFalpha-mediated apoptosis in a p53-dependent manner.
PMID:30089260
p53 Regulates the Expression of LRP1 and Apoptosis through a Stress Intensity-Dependent MicroRNA Feedback Loop.
PMID:7958916
DNA damage triggers a prolonged p53-dependent G1 arrest and long-term induction of Cip1 in normal human fibroblasts.
PMID:11672523
hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase.
PMID:17145718
Brn-3b enhances the pro-apoptotic effects of p53 but not its induction of cell cycle arrest by cooperating in trans-activation of bax expression.
PMID:17146433
Dec 4. RGC32, a novel p53-inducible gene, is located on centrosomes during mitosis and results in G2/M arrest.
PMID:18549481
TATA binding protein associated factor 3 (TAF3) interacts with p53 and inhibits its function.
PMID:24356969
2013 Dec 19. Rbm24, an RNA-binding protein and a target of p53, regulates p21 expression via mRNA stability.
PMID:17310983
Feb 19. Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function.
PMID:24652652
2014 Mar 20. DRAGO (KIAA0247), a new DNA damage-responsive, p53-inducible gene that cooperates with p53 as oncosuppressor.
PMID:20959462
2010 Oct 19. Aurora B interacts with NIR-p53, leading to p53 phosphorylation in its DNA-binding domain and subsequent functional suppression.
GO:0045944 positive regulation of transcription by RNA polymerase II
IDA
PMID:30089260
p53 Regulates the Expression of LRP1 and Apoptosis through a... 		ACCEPT
Summary: p53 is a sequence-specific transcriptional activator that upregulates target genes including p21, PUMA, BAX.
Reason: Fundamental molecular function - p53 activates transcription of numerous target genes through binding to p53 response elements.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:16061649
Ckap2 regulates aneuploidy, cell cycling, and cell death in a p53-dependent manner.
PMID:17599062
Jun 28. CDIP, a novel pro-apoptotic gene, regulates TNFalpha-mediated apoptosis in a p53-dependent manner.
PMID:30089260
p53 Regulates the Expression of LRP1 and Apoptosis through a Stress Intensity-Dependent MicroRNA Feedback Loop.
PMID:7958916
DNA damage triggers a prolonged p53-dependent G1 arrest and long-term induction of Cip1 in normal human fibroblasts.
PMID:11672523
hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase.
PMID:17145718
Brn-3b enhances the pro-apoptotic effects of p53 but not its induction of cell cycle arrest by cooperating in trans-activation of bax expression.
PMID:17146433
Dec 4. RGC32, a novel p53-inducible gene, is located on centrosomes during mitosis and results in G2/M arrest.
PMID:18549481
TATA binding protein associated factor 3 (TAF3) interacts with p53 and inhibits its function.
PMID:24356969
2013 Dec 19. Rbm24, an RNA-binding protein and a target of p53, regulates p21 expression via mRNA stability.
PMID:17310983
Feb 19. Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function.
PMID:24652652
2014 Mar 20. DRAGO (KIAA0247), a new DNA damage-responsive, p53-inducible gene that cooperates with p53 as oncosuppressor.
PMID:20959462
2010 Oct 19. Aurora B interacts with NIR-p53, leading to p53 phosphorylation in its DNA-binding domain and subsequent functional suppression.
GO:0045944 positive regulation of transcription by RNA polymerase II
IMP
PMID:7958916
DNA damage triggers a prolonged p53-dependent G1 arrest and ... 		ACCEPT
Summary: p53 is a sequence-specific transcriptional activator that upregulates target genes including p21, PUMA, BAX.
Reason: Fundamental molecular function - p53 activates transcription of numerous target genes through binding to p53 response elements.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:16061649
Ckap2 regulates aneuploidy, cell cycling, and cell death in a p53-dependent manner.
PMID:17599062
Jun 28. CDIP, a novel pro-apoptotic gene, regulates TNFalpha-mediated apoptosis in a p53-dependent manner.
PMID:30089260
p53 Regulates the Expression of LRP1 and Apoptosis through a Stress Intensity-Dependent MicroRNA Feedback Loop.
PMID:7958916
DNA damage triggers a prolonged p53-dependent G1 arrest and long-term induction of Cip1 in normal human fibroblasts.
PMID:11672523
hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase.
PMID:17145718
Brn-3b enhances the pro-apoptotic effects of p53 but not its induction of cell cycle arrest by cooperating in trans-activation of bax expression.
PMID:17146433
Dec 4. RGC32, a novel p53-inducible gene, is located on centrosomes during mitosis and results in G2/M arrest.
PMID:18549481
TATA binding protein associated factor 3 (TAF3) interacts with p53 and inhibits its function.
PMID:24356969
2013 Dec 19. Rbm24, an RNA-binding protein and a target of p53, regulates p21 expression via mRNA stability.
PMID:17310983
Feb 19. Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function.
PMID:24652652
2014 Mar 20. DRAGO (KIAA0247), a new DNA damage-responsive, p53-inducible gene that cooperates with p53 as oncosuppressor.
PMID:20959462
2010 Oct 19. Aurora B interacts with NIR-p53, leading to p53 phosphorylation in its DNA-binding domain and subsequent functional suppression.
GO:0005515 protein binding
IPI
PMID:24625977
Ubiquitin-like (UBX)-domain-containing protein, UBXN2A, prom... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:24625977
Ubiquitin-like (UBX)-domain-containing protein, UBXN2A, promotes cell death by interfering with the p53-Mortalin interactions in colon cancer cells.
GO:0005634 nucleus
IDA
PMID:24625977
Ubiquitin-like (UBX)-domain-containing protein, UBXN2A, prom... 		ACCEPT
Summary: p53 is primarily a nuclear transcription factor, with nuclear localization essential for its function.
Reason: Core cellular component - p53's nuclear localization is essential for its transcription factor activity. Contains nuclear localization signals at residues 305-322 and 369-375.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Endoplasmic reticulum.
PMID:14963330
Direct activation of Bax by p53 mediates mitochondrial membrane permeabilization and apoptosis.
PMID:16322561
NIR is a novel INHAT repressor that modulates the transcriptional activity of p53.
PMID:36108750
Phase separation of p53 induced by its unstructured basic region and prevented by oncogenic mutations in tetramerization domain.
PMID:24625977
Ubiquitin-like (UBX)-domain-containing protein, UBXN2A, promotes cell death by interfering with the p53-Mortalin interactions in colon cancer cells.
PMID:26634371
Epub 2015 Dec 4. Structural studies of UBXN2A and mortalin interaction and the putative role of silenced UBXN2A in preventing response to chemotherapy.
PMID:21597459
2011 May 20. E3 ubiquitin ligase Hades negatively regulates the exonuclear function of p53.
PMID:19011621
Arginine methylation regulates the p53 response.
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:24101517
Mitochondrial disulfide relay mediates translocation of p53 and partitions its subcellular activity.
PMID:20810912
Hypoxia downregulates p53 but induces apoptosis and enhances expression of BAD in cultures of human syncytiotrophoblasts.
PMID:19234109
Induction of SOX4 by DNA damage is critical for p53 stabilization and function.
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:17403783
HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53.
PMID:20096447
USP10 regulates p53 localization and stability by deubiquitinating p53.
PMID:18756595
Prognostic significance of BMP and activin membrane-bound inhibitor in colorectal cancer.
PMID:16507995
Myosin VI is a mediator of the p53-dependent cell survival pathway.
PMID:7720704
Direct involvement of p53 in programmed cell death of oligodendrocytes.
GO:0005634 nucleus
IDA
PMID:26634371
Structural studies of UBXN2A and mortalin interaction and th... 		ACCEPT
Summary: p53 is primarily a nuclear transcription factor, with nuclear localization essential for its function.
Reason: Core cellular component - p53's nuclear localization is essential for its transcription factor activity. Contains nuclear localization signals at residues 305-322 and 369-375.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Endoplasmic reticulum.
PMID:14963330
Direct activation of Bax by p53 mediates mitochondrial membrane permeabilization and apoptosis.
PMID:16322561
NIR is a novel INHAT repressor that modulates the transcriptional activity of p53.
PMID:36108750
Phase separation of p53 induced by its unstructured basic region and prevented by oncogenic mutations in tetramerization domain.
PMID:24625977
Ubiquitin-like (UBX)-domain-containing protein, UBXN2A, promotes cell death by interfering with the p53-Mortalin interactions in colon cancer cells.
PMID:26634371
Epub 2015 Dec 4. Structural studies of UBXN2A and mortalin interaction and the putative role of silenced UBXN2A in preventing response to chemotherapy.
PMID:21597459
2011 May 20. E3 ubiquitin ligase Hades negatively regulates the exonuclear function of p53.
PMID:19011621
Arginine methylation regulates the p53 response.
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:24101517
Mitochondrial disulfide relay mediates translocation of p53 and partitions its subcellular activity.
PMID:20810912
Hypoxia downregulates p53 but induces apoptosis and enhances expression of BAD in cultures of human syncytiotrophoblasts.
PMID:19234109
Induction of SOX4 by DNA damage is critical for p53 stabilization and function.
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:17403783
HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53.
PMID:20096447
USP10 regulates p53 localization and stability by deubiquitinating p53.
PMID:18756595
Prognostic significance of BMP and activin membrane-bound inhibitor in colorectal cancer.
PMID:16507995
Myosin VI is a mediator of the p53-dependent cell survival pathway.
PMID:7720704
Direct involvement of p53 in programmed cell death of oligodendrocytes.
GO:0005737 cytoplasm
IDA
PMID:24625977
Ubiquitin-like (UBX)-domain-containing protein, UBXN2A, prom... 		ACCEPT
Summary: p53 localizes to cytoplasm as well as nucleus, with cytoplasmic localization important for regulation.
Reason: Core cellular component - p53 shuttles between nucleus and cytoplasm, with cytoplasmic localization important for MDM2-mediated regulation.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus. Cytoplasm.
PMID:24625977
Ubiquitin-like (UBX)-domain-containing protein, UBXN2A, promotes cell death by interfering with the p53-Mortalin interactions in colon cancer cells.
PMID:26634371
Epub 2015 Dec 4. Structural studies of UBXN2A and mortalin interaction and the putative role of silenced UBXN2A in preventing response to chemotherapy.
PMID:21597459
2011 May 20. E3 ubiquitin ligase Hades negatively regulates the exonuclear function of p53.
PMID:19011621
Arginine methylation regulates the p53 response.
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:23629966
Deacetylation of p53 induces autophagy by suppressing Bmf expression.
PMID:20810912
Hypoxia downregulates p53 but induces apoptosis and enhances expression of BAD in cultures of human syncytiotrophoblasts.
PMID:16131611
p53 isoforms can regulate p53 transcriptional activity.
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:20096447
USP10 regulates p53 localization and stability by deubiquitinating p53.
PMID:7720704
Direct involvement of p53 in programmed cell death of oligodendrocytes.
GO:0005737 cytoplasm
IDA
PMID:26634371
Structural studies of UBXN2A and mortalin interaction and th... 		ACCEPT
Summary: p53 localizes to cytoplasm as well as nucleus, with cytoplasmic localization important for regulation.
Reason: Core cellular component - p53 shuttles between nucleus and cytoplasm, with cytoplasmic localization important for MDM2-mediated regulation.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus. Cytoplasm.
PMID:24625977
Ubiquitin-like (UBX)-domain-containing protein, UBXN2A, promotes cell death by interfering with the p53-Mortalin interactions in colon cancer cells.
PMID:26634371
Epub 2015 Dec 4. Structural studies of UBXN2A and mortalin interaction and the putative role of silenced UBXN2A in preventing response to chemotherapy.
PMID:21597459
2011 May 20. E3 ubiquitin ligase Hades negatively regulates the exonuclear function of p53.
PMID:19011621
Arginine methylation regulates the p53 response.
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:23629966
Deacetylation of p53 induces autophagy by suppressing Bmf expression.
PMID:20810912
Hypoxia downregulates p53 but induces apoptosis and enhances expression of BAD in cultures of human syncytiotrophoblasts.
PMID:16131611
p53 isoforms can regulate p53 transcriptional activity.
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:20096447
USP10 regulates p53 localization and stability by deubiquitinating p53.
PMID:7720704
Direct involvement of p53 in programmed cell death of oligodendrocytes.
GO:0003682 chromatin binding
IDA
PMID:26334721
Jmjd5 functions as a regulator of p53 signaling during mouse... 		ACCEPT
Summary: p53 binds chromatin at p53 response elements in target gene promoters.
Reason: Core molecular function - chromatin binding is essential for p53's transcriptional regulation.
Supporting Evidence:
PDB:3TS8
Crystal structure of p53 tetramer bound to DNA
PMID:26334721
2015 Sep 3. Jmjd5 functions as a regulator of p53 signaling during mouse embryogenesis.
PMID:16322561
NIR is a novel INHAT repressor that modulates the transcriptional activity of p53.
PMID:17599062
Jun 28. CDIP, a novel pro-apoptotic gene, regulates TNFalpha-mediated apoptosis in a p53-dependent manner.
GO:0003700 DNA-binding transcription factor activity
IDA
PMID:26334721
Jmjd5 functions as a regulator of p53 signaling during mouse... 		ACCEPT
Summary: p53 is a well-characterized DNA-binding transcription factor that activates and represses target genes.
Reason: Core molecular function - p53 is one of the best-characterized transcription factors, regulating >500 target genes.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:26334721
2015 Sep 3. Jmjd5 functions as a regulator of p53 signaling during mouse embryogenesis.
PMID:18549481
TATA binding protein associated factor 3 (TAF3) interacts with p53 and inhibits its function.
PMID:7587074
Targets for transcriptional activation by wild-type p53: endogenous retroviral LTR, immunoglobulin-like promoter, and an internal promoter of the mdm2 gene.
GO:0005515 protein binding
IPI
PMID:26334721
Jmjd5 functions as a regulator of p53 signaling during mouse... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:26334721
2015 Sep 3. Jmjd5 functions as a regulator of p53 signaling during mouse embryogenesis.
GO:0006357 regulation of transcription by RNA polymerase II
IDA
PMID:30089260
p53 Regulates the Expression of LRP1 and Apoptosis through a... 		ACCEPT
Summary: p53 regulates RNA polymerase II transcription as both activator and repressor.
Reason: Core molecular function - p53 is a sequence-specific RNA pol II transcription factor.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:30089260
p53 Regulates the Expression of LRP1 and Apoptosis through a Stress Intensity-Dependent MicroRNA Feedback Loop.
GO:0006974 DNA damage response
IDA
PMID:30089260
p53 Regulates the Expression of LRP1 and Apoptosis through a... 		ACCEPT
Summary: p53 is central to the DNA damage response, being activated by and coordinating responses to DNA damage.
Reason: Core function - p53 is the 'guardian of the genome' that coordinates DNA damage responses.
Supporting Evidence:
TP53-deep-research
p53 serves as a central node in the DNA damage response network
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:30089260
p53 Regulates the Expression of LRP1 and Apoptosis through a Stress Intensity-Dependent MicroRNA Feedback Loop.
PMID:24356969
2013 Dec 19. Rbm24, an RNA-binding protein and a target of p53, regulates p21 expression via mRNA stability.
PMID:17938203
Oct 15. Protein kinase C delta induces transcription of the TP53 tumor suppressor gene by controlling death-promoting factor Btf in the apoptotic response to DNA damage.
PMID:15710329
Human MUC1 oncoprotein regulates p53-responsive gene transcription in the genotoxic stress response.
GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding
IDA
PMID:24289924
Phosphorylation of p53 by TAF1 inactivates p53-dependent tra... 		ACCEPT
Summary: p53 binds sequence-specifically to p53 response elements in cis-regulatory regions of target genes.
Reason: Core molecular function - p53's DNA-binding domain binds to consensus p53 response elements (p53REs) with high specificity.
Supporting Evidence:
TP53-deep-research
TP53 is fundamentally a transcription factor that binds sequence-specifically to p53 response elements (p53REs) in target gene promoters
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:22578566
Apr 19. Oncosuppressive role of p53-induced miR-205 in triple negative breast cancer.
GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific
IDA
PMID:24289924
Phosphorylation of p53 by TAF1 inactivates p53-dependent tra... 		ACCEPT
Summary: p53 is a DNA-binding transcription factor that regulates RNA polymerase II transcription of target genes.
Reason: Core molecular function - p53 is a well-characterized sequence-specific transcription factor for RNA pol II.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:34381247
Epub 2021 Aug 11. The tumor suppressor folliculin inhibits lactate dehydrogenase A and regulates the Warburg effect.
PMID:35618207
Ser392 phosphorylation modulated a switch between p53 and transcriptional condensates.
PMID:36634798
Phosphorylation and specific DNA improved the incorporation ability of p53 into functional condensates.
PMID:38653238
2024 Apr 22. Alanyl-tRNA synthetase, AARS1, is a lactate sensor and lactyltransferase that lactylates p53 and contributes to tumorigenesis.
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:17310983
Feb 19. Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function.
PMID:24652652
2014 Mar 20. DRAGO (KIAA0247), a new DNA damage-responsive, p53-inducible gene that cooperates with p53 as oncosuppressor.
GO:0072331 signal transduction by p53 class mediator
IDA
PMID:29681526
A Designed Peptide Targets Two Types of Modifications of p53... 		ACCEPT
Summary: p53 IS the p53 class mediator that transduces signals in response to stress.
Reason: Absolutely core function - this term specifically describes p53's central role in stress signaling.
Supporting Evidence:
TP53-deep-research
p53 coordinates cellular responses to diverse stress signals
PMID:25384516
Rad54B serves as a scaffold in the DNA damage response that limits checkpoint strength.
PMID:18549481
TATA binding protein associated factor 3 (TAF3) interacts with p53 and inhibits its function.
PMID:29681526
A Designed Peptide Targets Two Types of Modifications of p53 with Anti-cancer Activity.
PMID:15314173
Ribosomal protein L23 activates p53 by inhibiting MDM2 function in response to ribosomal perturbation but not to translation inhibition.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-9836362 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005515 protein binding
IPI
PMID:35122041
Aldolase B suppresses hepatocellular carcinogenesis by inhib... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:35122041
Jul 6. Aldolase B suppresses hepatocellular carcinogenesis by inhibiting G6PD and pentose phosphate pathways.
GO:0005515 protein binding
IPI
PMID:10415337
Identification of a novel gene encoding a p53-associated pro... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:10415337
Identification of a novel gene encoding a p53-associated protein.
GO:0005515 protein binding
IPI
PMID:10666337
Human topoisomerase IIalpha and IIbeta interact with the C-t... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:10666337
Human topoisomerase IIalpha and IIbeta interact with the C-terminal region of p53.
GO:0005515 protein binding
IPI
PMID:11546806
Cloning and characterization of a p53-related protein kinase... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:11546806
2001 Sep 6. Cloning and characterization of a p53-related protein kinase expressed in interleukin-2-activated cytotoxic T-cells, epithelial tumor cell lines, and the testes.
GO:0005515 protein binding
IPI
PMID:11781842
The Bloom syndrome protein interacts and cooperates with p53... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:11781842
The Bloom syndrome protein interacts and cooperates with p53 in regulation of transcription and cell growth control.
GO:0005515 protein binding
IPI
PMID:15577914
Regulation of cellular response to oncogenic and oxidative s... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:15577914
Regulation of cellular response to oncogenic and oxidative stress by Seladin-1.
GO:0005515 protein binding
IPI
PMID:17121812
CARPs are ubiquitin ligases that promote MDM2-independent p5... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:17121812
Nov 22. CARPs are ubiquitin ligases that promote MDM2-independent p53 and phospho-p53ser20 degradation.
GO:0005515 protein binding
IPI
PMID:18382127
CARPs enhance p53 turnover by degrading 14-3-3sigma and stab... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:18382127
CARPs enhance p53 turnover by degrading 14-3-3sigma and stabilizing MDM2.
GO:0005515 protein binding
IPI
PMID:7663514
p53 modulation of TFIIH-associated nucleotide excision repai... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:7663514
p53 modulation of TFIIH-associated nucleotide excision repair activity.
GO:0005515 protein binding
IPI
PMID:9194565
Binding and modulation of p53 by p300/CBP coactivators. 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:9194565
Binding and modulation of p53 by p300/CBP coactivators.
GO:0005515 protein binding
IPI
PMID:9529249
ARF promotes MDM2 degradation and stabilizes p53: ARF-INK4a ... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:9529249
ARF promotes MDM2 degradation and stabilizes p53: ARF-INK4a locus deletion impairs both the Rb and p53 tumor suppression pathways.
GO:0010628 positive regulation of gene expression
IDA
PMID:26100857
A metabolic stress-inducible miR-34a-HNF4Ξ± pathway regulates... 		ACCEPT
Summary: p53 positively regulates gene expression of numerous target genes.
Reason: Core transcriptional function - p53 is a transcriptional activator.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:26100857
A metabolic stress-inducible miR-34a-HNF4Ξ± pathway regulates lipid and lipoprotein metabolism.
PMID:20332243
2010 Mar 23. MicroRNA145 targets BNIP3 and suppresses prostate cancer progression.
PMID:15314173
Ribosomal protein L23 activates p53 by inhibiting MDM2 function in response to ribosomal perturbation but not to translation inhibition.
PMID:19160485
A ribosomal protein L23-nucleophosmin circuit coordinates Mizl function with cell growth.
GO:0005515 protein binding
IPI
PMID:34404770
ZNF768 links oncogenic RAS to cellular senescence. 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:34404770
ZNF768 links oncogenic RAS to cellular senescence.
GO:0045815 transcription initiation-coupled chromatin remodeling
IDA
PMID:16322561
NIR is a novel INHAT repressor that modulates the transcript... 		ACCEPT
Summary: p53 positively regulates gene expression through transcriptional activation.
Reason: Core function - p53 is a transcriptional activator.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:16322561
NIR is a novel INHAT repressor that modulates the transcriptional activity of p53.
GO:0005515 protein binding
IPI
PMID:33591310
DAZAP2 acts as specifier of the p53 response to DNA damage. 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:33591310
DAZAP2 acts as specifier of the p53 response to DNA damage.
GO:0003700 DNA-binding transcription factor activity
IDA
PMID:18549481
TATA binding protein associated factor 3 (TAF3) interacts wi... 		ACCEPT
Summary: p53 is a well-characterized DNA-binding transcription factor that activates and represses target genes.
Reason: Core molecular function - p53 is one of the best-characterized transcription factors, regulating >500 target genes.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:26334721
2015 Sep 3. Jmjd5 functions as a regulator of p53 signaling during mouse embryogenesis.
PMID:18549481
TATA binding protein associated factor 3 (TAF3) interacts with p53 and inhibits its function.
PMID:7587074
Targets for transcriptional activation by wild-type p53: endogenous retroviral LTR, immunoglobulin-like promoter, and an internal promoter of the mdm2 gene.
GO:2000774 positive regulation of cellular senescence
IDA
PMID:29474172
Haploinsufficiency of Trp53 dramatically extends the lifespa... 		ACCEPT
Summary: p53 positively regulates cellular senescence as an alternative to apoptosis.
Reason: Core function - senescence induction is a major tumor suppressor mechanism of p53.
Supporting Evidence:
TP53-deep-research
p53 induces senescence through p21 and other targets as an irreversible cell cycle arrest mechanism
PMID:29474172
Haploinsufficiency of Trp53 dramatically extends the lifespan of Sirt6-deficient mice.
GO:0031625 ubiquitin protein ligase binding
EXP
PMID:8875929
Structure of the MDM2 oncoprotein bound to the p53 tumor sup... 		REMOVE
Summary: Generic 'ubiquitin protein ligase binding' is less informative than specific MDM2/MDM4 interactions.
Reason: While p53 binds MDM2 (E3 ligase), this generic term is uninformative. The specific MDM2 interaction is well-documented elsewhere.
Supporting Evidence:
PMID:8875929
Structure of the MDM2 oncoprotein bound to the p53 tumor suppressor transactivation domain.
GO:0071889 14-3-3 protein binding
EXP
PMID:20206173
Structure of the p53 C-terminus bound to 14-3-3: implication... 		ACCEPT
Summary: p53 binds to 14-3-3 proteins which regulate its subcellular localization.
Reason: Important regulatory interaction - 14-3-3 proteins sequester p53 in cytoplasm.
Supporting Evidence:
PMID:20206173
2010 Mar 3. Structure of the p53 C-terminus bound to 14-3-3: implications for stabilization of the p53 tetramer.
GO:0140677 molecular function activator activity
EXP
PMID:14759370
Structural mechanism of the bromodomain of the coactivator C... 		ACCEPT
Summary: p53 functions as an activating transcription factor for target genes.
Reason: Core molecular function - p53 activates transcription of target genes.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:14759370
Structural mechanism of the bromodomain of the coactivator CBP in p53 transcriptional activation.
PMID:16793543
Structure of the Tfb1/p53 complex: Insights into the interaction between the p62/Tfb1 subunit of TFIIH and the activation domain of p53.
PMID:7559631
Transactivation ability of p53 transcriptional activation domain is directly related to the binding affinity to TATA-binding protein.
GO:0140677 molecular function activator activity
EXP
PMID:16793543
Structure of the Tfb1/p53 complex: Insights into the interac... 		ACCEPT
Summary: p53 functions as an activating transcription factor for target genes.
Reason: Core molecular function - p53 activates transcription of target genes.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:14759370
Structural mechanism of the bromodomain of the coactivator CBP in p53 transcriptional activation.
PMID:16793543
Structure of the Tfb1/p53 complex: Insights into the interaction between the p62/Tfb1 subunit of TFIIH and the activation domain of p53.
PMID:7559631
Transactivation ability of p53 transcriptional activation domain is directly related to the binding affinity to TATA-binding protein.
GO:0140677 molecular function activator activity
IPI
PMID:7559631
Transactivation ability of p53 transcriptional activation do... 		ACCEPT
Summary: p53 functions as an activating transcription factor for target genes.
Reason: Core molecular function - p53 activates transcription of target genes.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:14759370
Structural mechanism of the bromodomain of the coactivator CBP in p53 transcriptional activation.
PMID:16793543
Structure of the Tfb1/p53 complex: Insights into the interaction between the p62/Tfb1 subunit of TFIIH and the activation domain of p53.
PMID:7559631
Transactivation ability of p53 transcriptional activation domain is directly related to the binding affinity to TATA-binding protein.
GO:2000774 positive regulation of cellular senescence
ISS
GO_REF:0000024 		ACCEPT
Summary: p53 positively regulates cellular senescence as an alternative to apoptosis.
Reason: Core function - senescence induction is a major tumor suppressor mechanism of p53.
Supporting Evidence:
TP53-deep-research
p53 induces senescence through p21 and other targets as an irreversible cell cycle arrest mechanism
PMID:29474172
Haploinsufficiency of Trp53 dramatically extends the lifespan of Sirt6-deficient mice.
GO:0090398 cellular senescence
IMP
PMID:12080348
Nucleophosmin regulates the stability and transcriptional ac... 		ACCEPT
Summary: p53 is negatively regulated by MDM2-mediated cellular homeostasis.
Reason: Core regulatory mechanism - p53 is tightly regulated in non-stressed cells.
Supporting Evidence:
TP53-deep-research
Negative Feedback: MDM2: Primary negative regulator
PMID:12080348
Nucleophosmin regulates the stability and transcriptional activity of p53.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-2997706 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005515 protein binding
IPI
PMID:15687255
A mechanism of ubiquitin-independent proteasomal degradation... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:15687255
A mechanism of ubiquitin-independent proteasomal degradation of the tumor suppressors p53 and p73.
GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific
IDA
PMID:22578566
Oncosuppressive role of p53-induced miR-205 in triple negati... 		ACCEPT
Summary: p53 activates transcription through binding to promoter sequences.
Reason: Core molecular function - p53 is a transcriptional activator that binds to p53 response elements.
Supporting Evidence:
TP53-deep-research
TP53 is fundamentally a transcription factor that binds sequence-specifically to p53 response elements
PMID:22578566
Apr 19. Oncosuppressive role of p53-induced miR-205 in triple negative breast cancer.
PMID:17145718
Brn-3b enhances the pro-apoptotic effects of p53 but not its induction of cell cycle arrest by cooperating in trans-activation of bax expression.
PMID:12609999
2003 Feb 27. The activation domains, the proline-rich domain, and the C-terminal basic domain in p53 are necessary for acetylation of histones on the proximal p21 promoter and interaction with p300/CREB-binding protein.
PMID:17146433
Dec 4. RGC32, a novel p53-inducible gene, is located on centrosomes during mitosis and results in G2/M arrest.
GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding
IDA
PMID:22578566
Oncosuppressive role of p53-induced miR-205 in triple negati... 		ACCEPT
Summary: p53 binds sequence-specifically to p53 response elements in cis-regulatory regions of target genes.
Reason: Core molecular function - p53's DNA-binding domain binds to consensus p53 response elements (p53REs) with high specificity.
Supporting Evidence:
TP53-deep-research
TP53 is fundamentally a transcription factor that binds sequence-specifically to p53 response elements (p53REs) in target gene promoters
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:22578566
Apr 19. Oncosuppressive role of p53-induced miR-205 in triple negative breast cancer.
GO:1902895 positive regulation of miRNA transcription
IMP
PMID:22578566
Oncosuppressive role of p53-induced miR-205 in triple negati... 		ACCEPT
Summary: p53 transcriptionally regulates multiple miRNAs including miR-34 family.
Reason: p53 regulates miRNA expression as part of its stress response functions.
Supporting Evidence:
PMID:30089260
we found p53-dependent induction of these miRNAs upon nutlin-3a treatment
PMID:22578566
Apr 19. Oncosuppressive role of p53-induced miR-205 in triple negative breast cancer.
PMID:20546595
High-throughput sequencing identifies STAT3 as the DNA-associated factor for p53-NF-kappaB-complex-dependent gene expression in human heart failure.
PMID:22499991
Downregulation of endothelial microRNA-200b supports cutaneous wound angiogenesis by desilencing GATA binding protein 2 and vascular endothelial growth factor receptor 2.
GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific
IDA
PMID:17145718
Brn-3b enhances the pro-apoptotic effects of p53 but not its... 		ACCEPT
Summary: p53 activates transcription through binding to promoter sequences.
Reason: Core molecular function - p53 is a transcriptional activator that binds to p53 response elements.
Supporting Evidence:
TP53-deep-research
TP53 is fundamentally a transcription factor that binds sequence-specifically to p53 response elements
PMID:22578566
Apr 19. Oncosuppressive role of p53-induced miR-205 in triple negative breast cancer.
PMID:17145718
Brn-3b enhances the pro-apoptotic effects of p53 but not its induction of cell cycle arrest by cooperating in trans-activation of bax expression.
PMID:12609999
2003 Feb 27. The activation domains, the proline-rich domain, and the C-terminal basic domain in p53 are necessary for acetylation of histones on the proximal p21 promoter and interaction with p300/CREB-binding protein.
PMID:17146433
Dec 4. RGC32, a novel p53-inducible gene, is located on centrosomes during mitosis and results in G2/M arrest.
GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding
IPI
PMID:23329847
Regulation of the cyclin-dependent kinase inhibitor 1A gene ... 		ACCEPT
Summary: p53 binds RNA through its C-terminal domain.
Reason: p53 has RNA-binding activity that contributes to regulation of gene expression.
Supporting Evidence:
PMID:23329847
2013 Jan 17. Regulation of the cyclin-dependent kinase inhibitor 1A gene (CDKN1A) by the repressor BOZF1 through inhibition of p53 acetylation and transcription factor Sp1 binding.
GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding
IPI
PMID:15705871
Homeobox Msx1 interacts with p53 tumor suppressor and inhibi... 		ACCEPT
Summary: p53 binds RNA through its C-terminal domain.
Reason: p53 has RNA-binding activity that contributes to regulation of gene expression.
Supporting Evidence:
PMID:15705871
Homeobox Msx1 interacts with p53 tumor suppressor and inhibits tumor growth by inducing apoptosis.
GO:0051726 regulation of cell cycle
IGI
PMID:12203124
The Brn-3a transcription factor inhibits the pro-apoptotic e... 		ACCEPT
Summary: p53 is a master regulator of the cell cycle, inducing arrest at G1/S and G2/M checkpoints.
Reason: Core function - cell cycle regulation is fundamental to p53's tumor suppressor activity.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
induces cell cycle arrest
PMID:30514107
CELF1/p53 axis: a sustained antiproliferative signal leading to villus atrophy under total parenteral nutrition.
PMID:12203124
The Brn-3a transcription factor inhibits the pro-apoptotic effect of p53 and enhances cell cycle arrest by differentially regulating the activity of the p53 target genes encoding Bax and p21(CIP1/Waf1).
PMID:12433990
Distinct promoter elements mediate the co-operative effect of Brn-3a and p53 on the p21 promoter and their antagonism on the Bax promoter.
PMID:15149599
Telomere shortening triggers senescence of human cells through a pathway involving ATM, p53, and p21(CIP1), but not p16(INK4a).
PMID:7958916
DNA damage triggers a prolonged p53-dependent G1 arrest and long-term induction of Cip1 in normal human fibroblasts.
GO:0051726 regulation of cell cycle
IGI
PMID:12433990
Distinct promoter elements mediate the co-operative effect o... 		ACCEPT
Summary: p53 is a master regulator of the cell cycle, inducing arrest at G1/S and G2/M checkpoints.
Reason: Core function - cell cycle regulation is fundamental to p53's tumor suppressor activity.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
induces cell cycle arrest
PMID:30514107
CELF1/p53 axis: a sustained antiproliferative signal leading to villus atrophy under total parenteral nutrition.
PMID:12203124
The Brn-3a transcription factor inhibits the pro-apoptotic effect of p53 and enhances cell cycle arrest by differentially regulating the activity of the p53 target genes encoding Bax and p21(CIP1/Waf1).
PMID:12433990
Distinct promoter elements mediate the co-operative effect of Brn-3a and p53 on the p21 promoter and their antagonism on the Bax promoter.
PMID:15149599
Telomere shortening triggers senescence of human cells through a pathway involving ATM, p53, and p21(CIP1), but not p16(INK4a).
PMID:7958916
DNA damage triggers a prolonged p53-dependent G1 arrest and long-term induction of Cip1 in normal human fibroblasts.
GO:0051726 regulation of cell cycle
IDA
PMID:15149599
Telomere shortening triggers senescence of human cells throu... 		ACCEPT
Summary: p53 is a master regulator of the cell cycle, inducing arrest at G1/S and G2/M checkpoints.
Reason: Core function - cell cycle regulation is fundamental to p53's tumor suppressor activity.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
induces cell cycle arrest
PMID:30514107
CELF1/p53 axis: a sustained antiproliferative signal leading to villus atrophy under total parenteral nutrition.
PMID:12203124
The Brn-3a transcription factor inhibits the pro-apoptotic effect of p53 and enhances cell cycle arrest by differentially regulating the activity of the p53 target genes encoding Bax and p21(CIP1/Waf1).
PMID:12433990
Distinct promoter elements mediate the co-operative effect of Brn-3a and p53 on the p21 promoter and their antagonism on the Bax promoter.
PMID:15149599
Telomere shortening triggers senescence of human cells through a pathway involving ATM, p53, and p21(CIP1), but not p16(INK4a).
PMID:7958916
DNA damage triggers a prolonged p53-dependent G1 arrest and long-term induction of Cip1 in normal human fibroblasts.
GO:0051726 regulation of cell cycle
IMP
PMID:7958916
DNA damage triggers a prolonged p53-dependent G1 arrest and ... 		ACCEPT
Summary: p53 is a master regulator of the cell cycle, inducing arrest at G1/S and G2/M checkpoints.
Reason: Core function - cell cycle regulation is fundamental to p53's tumor suppressor activity.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
induces cell cycle arrest
PMID:30514107
CELF1/p53 axis: a sustained antiproliferative signal leading to villus atrophy under total parenteral nutrition.
PMID:12203124
The Brn-3a transcription factor inhibits the pro-apoptotic effect of p53 and enhances cell cycle arrest by differentially regulating the activity of the p53 target genes encoding Bax and p21(CIP1/Waf1).
PMID:12433990
Distinct promoter elements mediate the co-operative effect of Brn-3a and p53 on the p21 promoter and their antagonism on the Bax promoter.
PMID:15149599
Telomere shortening triggers senescence of human cells through a pathway involving ATM, p53, and p21(CIP1), but not p16(INK4a).
PMID:7958916
DNA damage triggers a prolonged p53-dependent G1 arrest and long-term induction of Cip1 in normal human fibroblasts.
GO:0000122 negative regulation of transcription by RNA polymerase II
IDA
PMID:10329733
p53 suppresses the activation of the Bcl-2 promoter by the B... 		ACCEPT
Summary: p53 functions as a transcriptional repressor for genes including BCL2 and cell cycle genes.
Reason: Core function - transcriptional repression is part of p53's regulatory activity.
Supporting Evidence:
TP53-deep-research
Repression: Downregulates anti-apoptotic genes (BCL2), cell cycle progression genes
PMID:10329733
p53 suppresses the activation of the Bcl-2 promoter by the Brn-3a POU family transcription factor.
PMID:19749791
Sep 14. Repression of SHP-1 expression by p53 leads to trkA tyrosine phosphorylation and suppression of breast cancer cell proliferation.
GO:0000987 cis-regulatory region sequence-specific DNA binding
IDA
PMID:10329733
p53 suppresses the activation of the Bcl-2 promoter by the B... 		ACCEPT
Summary: p53 binds to proximal promoter sequences containing p53 response elements.
Reason: Core molecular function - DNA binding to promoters is essential for p53 activity.
Supporting Evidence:
TP53-deep-research
p53 binds sequence-specifically to p53 response elements in target gene promoters
PMID:10329733
p53 suppresses the activation of the Bcl-2 promoter by the Brn-3a POU family transcription factor.
GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific
IDA
PMID:10329733
p53 suppresses the activation of the Bcl-2 promoter by the B... 		ACCEPT
Summary: p53 functions as a transcriptional repressor at specific promoters.
Reason: Core function - p53 represses transcription of target genes.
Supporting Evidence:
TP53-deep-research
Repression: Downregulates anti-apoptotic genes (BCL2)
PMID:10329733
p53 suppresses the activation of the Bcl-2 promoter by the Brn-3a POU family transcription factor.
GO:1903451 negative regulation of G1 to G0 transition
IDA
PMID:15314173
Ribosomal protein L23 activates p53 by inhibiting MDM2 funct... 		ACCEPT
Summary: p53 negatively regulates ferroptosis through p21 induction and GSH maintenance.
Reason: p53 has dual role in ferroptosis - this represents its anti-ferroptotic activity.
Supporting Evidence:
TP53-deep-research
Anti-ferroptotic: Induces p21 to maintain GSH levels
PMID:15314173
Ribosomal protein L23 activates p53 by inhibiting MDM2 function in response to ribosomal perturbation but not to translation inhibition.
PMID:21471221
2011 Apr 6. Novel nucleolar pathway connecting intracellular energy status with p53 activation.
GO:1903451 negative regulation of G1 to G0 transition
IMP
PMID:21471221
Novel nucleolar pathway connecting intracellular energy stat... 		ACCEPT
Summary: p53 negatively regulates ferroptosis through p21 induction and GSH maintenance.
Reason: p53 has dual role in ferroptosis - this represents its anti-ferroptotic activity.
Supporting Evidence:
TP53-deep-research
Anti-ferroptotic: Induces p21 to maintain GSH levels
PMID:15314173
Ribosomal protein L23 activates p53 by inhibiting MDM2 function in response to ribosomal perturbation but not to translation inhibition.
PMID:21471221
2011 Apr 6. Novel nucleolar pathway connecting intracellular energy status with p53 activation.
GO:0005515 protein binding
IPI
PMID:25168243
Cofactor Strap regulates oxidative phosphorylation and mitoc... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:25168243
Aug 29. Cofactor Strap regulates oxidative phosphorylation and mitochondrial p53 activity through ATP synthase.
GO:0005739 mitochondrion
IDA
PMID:25168243
Cofactor Strap regulates oxidative phosphorylation and mitoc... 		ACCEPT
Summary: p53 translocates to mitochondria to directly promote apoptosis through BAX activation.
Reason: Important localization for non-transcriptional apoptosis - p53 can directly induce MOMP at mitochondria.
Supporting Evidence:
TP53-deep-research
Non-transcriptional: Direct mitochondrial translocation and interaction with BCL-2 family proteins
PMID:25168243
Aug 29. Cofactor Strap regulates oxidative phosphorylation and mitochondrial p53 activity through ATP synthase.
PMID:24101517
Mitochondrial disulfide relay mediates translocation of p53 and partitions its subcellular activity.
PMID:12667443
p53 has a direct apoptogenic role at the mitochondria.
GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding
IPI
PMID:19505873
Complementary quantitative proteomics reveals that transcrip... 		ACCEPT
Summary: p53 binds RNA through its C-terminal domain.
Reason: p53 has RNA-binding activity that contributes to regulation of gene expression.
Supporting Evidence:
PMID:19505873
Epub 2009 Jun 7. Complementary quantitative proteomics reveals that transcription factor AP-4 mediates E-box-dependent complex formation for transcriptional repression of HDM2.
GO:0036310 ATP-dependent DNA/DNA annealing activity
IDA
PMID:8183576
Human p53 directs DNA strand reassociation and is photolabel... 		ACCEPT
Summary: p53 responds to chemotherapy drugs that cause DNA damage.
Reason: Core function - DNA-damaging chemotherapeutics strongly activate p53.
Supporting Evidence:
PMID:8183576
Human p53 directs DNA strand reassociation and is photolabelled by 8-azido ATP.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-9723906 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0140296 general transcription initiation factor binding
IPI
PMID:15053879
Phosphorylation on Thr-55 by TAF1 mediates degradation of p5... 		ACCEPT
Summary: p53 binds chromatin to regulate transcription of target genes.
Reason: Core function - chromatin binding is essential for p53's transcription factor activity.
Supporting Evidence:
PDB:3TS8
Crystal structure shows p53 bound to DNA
PMID:15053879
Phosphorylation on Thr-55 by TAF1 mediates degradation of p53: a role for TAF1 in cell G1 progression.
GO:0010628 positive regulation of gene expression
IDA
PMID:20332243
MicroRNA145 targets BNIP3 and suppresses prostate cancer pro... 		ACCEPT
Summary: p53 positively regulates gene expression of numerous target genes.
Reason: Core transcriptional function - p53 is a transcriptional activator.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:26100857
A metabolic stress-inducible miR-34a-HNF4Ξ± pathway regulates lipid and lipoprotein metabolism.
PMID:20332243
2010 Mar 23. MicroRNA145 targets BNIP3 and suppresses prostate cancer progression.
PMID:15314173
Ribosomal protein L23 activates p53 by inhibiting MDM2 function in response to ribosomal perturbation but not to translation inhibition.
PMID:19160485
A ribosomal protein L23-nucleophosmin circuit coordinates Mizl function with cell growth.
GO:1902895 positive regulation of miRNA transcription
IDA
PMID:20546595
High-throughput sequencing identifies STAT3 as the DNA-assoc... 		ACCEPT
Summary: p53 transcriptionally regulates multiple miRNAs including miR-34 family.
Reason: p53 regulates miRNA expression as part of its stress response functions.
Supporting Evidence:
PMID:30089260
we found p53-dependent induction of these miRNAs upon nutlin-3a treatment
PMID:22578566
Apr 19. Oncosuppressive role of p53-induced miR-205 in triple negative breast cancer.
PMID:20546595
High-throughput sequencing identifies STAT3 as the DNA-associated factor for p53-NF-kappaB-complex-dependent gene expression in human heart failure.
PMID:22499991
Downregulation of endothelial microRNA-200b supports cutaneous wound angiogenesis by desilencing GATA binding protein 2 and vascular endothelial growth factor receptor 2.
GO:0000785 chromatin
IMP
PMID:30089260
p53 Regulates the Expression of LRP1 and Apoptosis through a... 		ACCEPT
Summary: p53 associates with chromatin at target gene promoters to regulate transcription.
Reason: Core molecular function - p53 binds to chromatin at p53 response elements to regulate target gene expression.
Supporting Evidence:
PDB:3TS8
Crystal structure of a multidomain human p53 tetramer bound to the natural CDKN1A(p21) p53-response element
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:30089260
p53 Regulates the Expression of LRP1 and Apoptosis through a Stress Intensity-Dependent MicroRNA Feedback Loop.
PMID:23629966
Deacetylation of p53 induces autophagy by suppressing Bmf expression.
PMID:22521434
Hepatocyte growth factor (HGF) and 1,25-dihydroxyvitamin D together stimulate human bone marrow-derived stem cells toward the osteogenic phenotype by HGF-induced up-regulation of VDR.
PMID:15710329
Human MUC1 oncoprotein regulates p53-responsive gene transcription in the genotoxic stress response.
PMID:17805299
p53 is regulated by the lysine demethylase LSD1.
GO:1902895 positive regulation of miRNA transcription
IGI
PMID:22499991
Downregulation of endothelial microRNA-200b supports cutaneo... 		ACCEPT
Summary: p53 transcriptionally regulates multiple miRNAs including miR-34 family.
Reason: p53 regulates miRNA expression as part of its stress response functions.
Supporting Evidence:
PMID:30089260
we found p53-dependent induction of these miRNAs upon nutlin-3a treatment
PMID:22578566
Apr 19. Oncosuppressive role of p53-induced miR-205 in triple negative breast cancer.
PMID:20546595
High-throughput sequencing identifies STAT3 as the DNA-associated factor for p53-NF-kappaB-complex-dependent gene expression in human heart failure.
PMID:22499991
Downregulation of endothelial microRNA-200b supports cutaneous wound angiogenesis by desilencing GATA binding protein 2 and vascular endothelial growth factor receptor 2.
GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific
IDA
PMID:12609999
The activation domains, the proline-rich domain, and the C-t... 		ACCEPT
Summary: p53 activates transcription through binding to promoter sequences.
Reason: Core molecular function - p53 is a transcriptional activator that binds to p53 response elements.
Supporting Evidence:
TP53-deep-research
TP53 is fundamentally a transcription factor that binds sequence-specifically to p53 response elements
PMID:22578566
Apr 19. Oncosuppressive role of p53-induced miR-205 in triple negative breast cancer.
PMID:17145718
Brn-3b enhances the pro-apoptotic effects of p53 but not its induction of cell cycle arrest by cooperating in trans-activation of bax expression.
PMID:12609999
2003 Feb 27. The activation domains, the proline-rich domain, and the C-terminal basic domain in p53 are necessary for acetylation of histones on the proximal p21 promoter and interaction with p300/CREB-binding protein.
PMID:17146433
Dec 4. RGC32, a novel p53-inducible gene, is located on centrosomes during mitosis and results in G2/M arrest.
GO:0043066 negative regulation of apoptotic process
IGI
PMID:12203124
The Brn-3a transcription factor inhibits the pro-apoptotic e... 		ACCEPT
Summary: p53 can negatively regulate apoptosis in certain contexts through p21-mediated survival.
Reason: Context-dependent function - p53 can promote survival under mild stress conditions.
Supporting Evidence:
PMID:12203124
The Brn-3a transcription factor inhibits the pro-apoptotic effect of p53 and enhances cell cycle arrest by differentially regulating the activity of the p53 target genes encoding Bax and p21(CIP1/Waf1).
GO:0043066 negative regulation of apoptotic process
IGI
PMID:12433990
Distinct promoter elements mediate the co-operative effect o... 		ACCEPT
Summary: p53 can negatively regulate apoptosis in certain contexts through p21-mediated survival.
Reason: Context-dependent function - p53 can promote survival under mild stress conditions.
Supporting Evidence:
PMID:12433990
Distinct promoter elements mediate the co-operative effect of Brn-3a and p53 on the p21 promoter and their antagonism on the Bax promoter.
GO:0005515 protein binding
IPI
PMID:21597459
E3 ubiquitin ligase Hades negatively regulates the exonuclea... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:21597459
2011 May 20. E3 ubiquitin ligase Hades negatively regulates the exonuclear function of p53.
GO:0005634 nucleus
IDA
PMID:21597459
E3 ubiquitin ligase Hades negatively regulates the exonuclea... 		ACCEPT
Summary: p53 is primarily a nuclear transcription factor, with nuclear localization essential for its function.
Reason: Core cellular component - p53's nuclear localization is essential for its transcription factor activity. Contains nuclear localization signals at residues 305-322 and 369-375.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Endoplasmic reticulum.
PMID:14963330
Direct activation of Bax by p53 mediates mitochondrial membrane permeabilization and apoptosis.
PMID:16322561
NIR is a novel INHAT repressor that modulates the transcriptional activity of p53.
PMID:36108750
Phase separation of p53 induced by its unstructured basic region and prevented by oncogenic mutations in tetramerization domain.
PMID:24625977
Ubiquitin-like (UBX)-domain-containing protein, UBXN2A, promotes cell death by interfering with the p53-Mortalin interactions in colon cancer cells.
PMID:26634371
Epub 2015 Dec 4. Structural studies of UBXN2A and mortalin interaction and the putative role of silenced UBXN2A in preventing response to chemotherapy.
PMID:21597459
2011 May 20. E3 ubiquitin ligase Hades negatively regulates the exonuclear function of p53.
PMID:19011621
Arginine methylation regulates the p53 response.
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:24101517
Mitochondrial disulfide relay mediates translocation of p53 and partitions its subcellular activity.
PMID:20810912
Hypoxia downregulates p53 but induces apoptosis and enhances expression of BAD in cultures of human syncytiotrophoblasts.
PMID:19234109
Induction of SOX4 by DNA damage is critical for p53 stabilization and function.
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:17403783
HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53.
PMID:20096447
USP10 regulates p53 localization and stability by deubiquitinating p53.
PMID:18756595
Prognostic significance of BMP and activin membrane-bound inhibitor in colorectal cancer.
PMID:16507995
Myosin VI is a mediator of the p53-dependent cell survival pathway.
PMID:7720704
Direct involvement of p53 in programmed cell death of oligodendrocytes.
GO:0005737 cytoplasm
IDA
PMID:21597459
E3 ubiquitin ligase Hades negatively regulates the exonuclea... 		ACCEPT
Summary: p53 localizes to cytoplasm as well as nucleus, with cytoplasmic localization important for regulation.
Reason: Core cellular component - p53 shuttles between nucleus and cytoplasm, with cytoplasmic localization important for MDM2-mediated regulation.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus. Cytoplasm.
PMID:24625977
Ubiquitin-like (UBX)-domain-containing protein, UBXN2A, promotes cell death by interfering with the p53-Mortalin interactions in colon cancer cells.
PMID:26634371
Epub 2015 Dec 4. Structural studies of UBXN2A and mortalin interaction and the putative role of silenced UBXN2A in preventing response to chemotherapy.
PMID:21597459
2011 May 20. E3 ubiquitin ligase Hades negatively regulates the exonuclear function of p53.
PMID:19011621
Arginine methylation regulates the p53 response.
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:23629966
Deacetylation of p53 induces autophagy by suppressing Bmf expression.
PMID:20810912
Hypoxia downregulates p53 but induces apoptosis and enhances expression of BAD in cultures of human syncytiotrophoblasts.
PMID:16131611
p53 isoforms can regulate p53 transcriptional activity.
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:20096447
USP10 regulates p53 localization and stability by deubiquitinating p53.
PMID:7720704
Direct involvement of p53 in programmed cell death of oligodendrocytes.
GO:0005515 protein binding
IPI
PMID:29628311
A Family of Vertebrate-Specific Polycombs Encoded by the LCO... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:29628311
2018 Apr 5. A Family of Vertebrate-Specific Polycombs Encoded by the LCOR/LCORL Genes Balance PRC2 Subtype Activities.
GO:0045899 positive regulation of RNA polymerase II transcription preinitiation complex assembly
IDA
PMID:24289924
Phosphorylation of p53 by TAF1 inactivates p53-dependent tra... 		ACCEPT
Summary: p53 positively regulates its own transcription as part of feedback regulation.
Reason: p53 has positive feedback regulation of its own expression.
Supporting Evidence:
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
GO:0045944 positive regulation of transcription by RNA polymerase II
IDA
PMID:11672523
hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase. 		ACCEPT
Summary: p53 is a sequence-specific transcriptional activator that upregulates target genes including p21, PUMA, BAX.
Reason: Fundamental molecular function - p53 activates transcription of numerous target genes through binding to p53 response elements.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:16061649
Ckap2 regulates aneuploidy, cell cycling, and cell death in a p53-dependent manner.
PMID:17599062
Jun 28. CDIP, a novel pro-apoptotic gene, regulates TNFalpha-mediated apoptosis in a p53-dependent manner.
PMID:30089260
p53 Regulates the Expression of LRP1 and Apoptosis through a Stress Intensity-Dependent MicroRNA Feedback Loop.
PMID:7958916
DNA damage triggers a prolonged p53-dependent G1 arrest and long-term induction of Cip1 in normal human fibroblasts.
PMID:11672523
hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase.
PMID:17145718
Brn-3b enhances the pro-apoptotic effects of p53 but not its induction of cell cycle arrest by cooperating in trans-activation of bax expression.
PMID:17146433
Dec 4. RGC32, a novel p53-inducible gene, is located on centrosomes during mitosis and results in G2/M arrest.
PMID:18549481
TATA binding protein associated factor 3 (TAF3) interacts with p53 and inhibits its function.
PMID:24356969
2013 Dec 19. Rbm24, an RNA-binding protein and a target of p53, regulates p21 expression via mRNA stability.
PMID:17310983
Feb 19. Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function.
PMID:24652652
2014 Mar 20. DRAGO (KIAA0247), a new DNA damage-responsive, p53-inducible gene that cooperates with p53 as oncosuppressor.
PMID:20959462
2010 Oct 19. Aurora B interacts with NIR-p53, leading to p53 phosphorylation in its DNA-binding domain and subsequent functional suppression.
GO:0045944 positive regulation of transcription by RNA polymerase II
IDA
PMID:17145718
Brn-3b enhances the pro-apoptotic effects of p53 but not its... 		ACCEPT
Summary: p53 is a sequence-specific transcriptional activator that upregulates target genes including p21, PUMA, BAX.
Reason: Fundamental molecular function - p53 activates transcription of numerous target genes through binding to p53 response elements.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:16061649
Ckap2 regulates aneuploidy, cell cycling, and cell death in a p53-dependent manner.
PMID:17599062
Jun 28. CDIP, a novel pro-apoptotic gene, regulates TNFalpha-mediated apoptosis in a p53-dependent manner.
PMID:30089260
p53 Regulates the Expression of LRP1 and Apoptosis through a Stress Intensity-Dependent MicroRNA Feedback Loop.
PMID:7958916
DNA damage triggers a prolonged p53-dependent G1 arrest and long-term induction of Cip1 in normal human fibroblasts.
PMID:11672523
hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase.
PMID:17145718
Brn-3b enhances the pro-apoptotic effects of p53 but not its induction of cell cycle arrest by cooperating in trans-activation of bax expression.
PMID:17146433
Dec 4. RGC32, a novel p53-inducible gene, is located on centrosomes during mitosis and results in G2/M arrest.
PMID:18549481
TATA binding protein associated factor 3 (TAF3) interacts with p53 and inhibits its function.
PMID:24356969
2013 Dec 19. Rbm24, an RNA-binding protein and a target of p53, regulates p21 expression via mRNA stability.
PMID:17310983
Feb 19. Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function.
PMID:24652652
2014 Mar 20. DRAGO (KIAA0247), a new DNA damage-responsive, p53-inducible gene that cooperates with p53 as oncosuppressor.
PMID:20959462
2010 Oct 19. Aurora B interacts with NIR-p53, leading to p53 phosphorylation in its DNA-binding domain and subsequent functional suppression.
GO:0045944 positive regulation of transcription by RNA polymerase II
IMP
PMID:17146433
RGC32, a novel p53-inducible gene, is located on centrosomes... 		ACCEPT
Summary: p53 is a sequence-specific transcriptional activator that upregulates target genes including p21, PUMA, BAX.
Reason: Fundamental molecular function - p53 activates transcription of numerous target genes through binding to p53 response elements.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:16061649
Ckap2 regulates aneuploidy, cell cycling, and cell death in a p53-dependent manner.
PMID:17599062
Jun 28. CDIP, a novel pro-apoptotic gene, regulates TNFalpha-mediated apoptosis in a p53-dependent manner.
PMID:30089260
p53 Regulates the Expression of LRP1 and Apoptosis through a Stress Intensity-Dependent MicroRNA Feedback Loop.
PMID:7958916
DNA damage triggers a prolonged p53-dependent G1 arrest and long-term induction of Cip1 in normal human fibroblasts.
PMID:11672523
hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase.
PMID:17145718
Brn-3b enhances the pro-apoptotic effects of p53 but not its induction of cell cycle arrest by cooperating in trans-activation of bax expression.
PMID:17146433
Dec 4. RGC32, a novel p53-inducible gene, is located on centrosomes during mitosis and results in G2/M arrest.
PMID:18549481
TATA binding protein associated factor 3 (TAF3) interacts with p53 and inhibits its function.
PMID:24356969
2013 Dec 19. Rbm24, an RNA-binding protein and a target of p53, regulates p21 expression via mRNA stability.
PMID:17310983
Feb 19. Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function.
PMID:24652652
2014 Mar 20. DRAGO (KIAA0247), a new DNA damage-responsive, p53-inducible gene that cooperates with p53 as oncosuppressor.
PMID:20959462
2010 Oct 19. Aurora B interacts with NIR-p53, leading to p53 phosphorylation in its DNA-binding domain and subsequent functional suppression.
GO:0045944 positive regulation of transcription by RNA polymerase II
IDA
PMID:18549481
TATA binding protein associated factor 3 (TAF3) interacts wi... 		ACCEPT
Summary: p53 is a sequence-specific transcriptional activator that upregulates target genes including p21, PUMA, BAX.
Reason: Fundamental molecular function - p53 activates transcription of numerous target genes through binding to p53 response elements.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:16061649
Ckap2 regulates aneuploidy, cell cycling, and cell death in a p53-dependent manner.
PMID:17599062
Jun 28. CDIP, a novel pro-apoptotic gene, regulates TNFalpha-mediated apoptosis in a p53-dependent manner.
PMID:30089260
p53 Regulates the Expression of LRP1 and Apoptosis through a Stress Intensity-Dependent MicroRNA Feedback Loop.
PMID:7958916
DNA damage triggers a prolonged p53-dependent G1 arrest and long-term induction of Cip1 in normal human fibroblasts.
PMID:11672523
hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase.
PMID:17145718
Brn-3b enhances the pro-apoptotic effects of p53 but not its induction of cell cycle arrest by cooperating in trans-activation of bax expression.
PMID:17146433
Dec 4. RGC32, a novel p53-inducible gene, is located on centrosomes during mitosis and results in G2/M arrest.
PMID:18549481
TATA binding protein associated factor 3 (TAF3) interacts with p53 and inhibits its function.
PMID:24356969
2013 Dec 19. Rbm24, an RNA-binding protein and a target of p53, regulates p21 expression via mRNA stability.
PMID:17310983
Feb 19. Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function.
PMID:24652652
2014 Mar 20. DRAGO (KIAA0247), a new DNA damage-responsive, p53-inducible gene that cooperates with p53 as oncosuppressor.
PMID:20959462
2010 Oct 19. Aurora B interacts with NIR-p53, leading to p53 phosphorylation in its DNA-binding domain and subsequent functional suppression.
GO:0033209 tumor necrosis factor-mediated signaling pathway
IGI
PMID:22499991
Downregulation of endothelial microRNA-200b supports cutaneo... 		ACCEPT
Summary: p53 responds to tumor necrosis factor signaling.
Reason: p53 interacts with TNF signaling pathways in cell death regulation.
Supporting Evidence:
PMID:22499991
Downregulation of endothelial microRNA-200b supports cutaneous wound angiogenesis by desilencing GATA binding protein 2 and vascular endothelial growth factor receptor 2.
GO:0001046 core promoter sequence-specific DNA binding
IDA
PMID:23629966
Deacetylation of p53 induces autophagy by suppressing Bmf ex... 		ACCEPT
Summary: p53 activates transcription through core promoter binding.
Reason: Core molecular function - p53 binds promoters to activate transcription.
Supporting Evidence:
PDB:3TS8
p53 tetramer bound to CDKN1A promoter response element
PMID:23629966
Deacetylation of p53 induces autophagy by suppressing Bmf expression.
GO:0042802 identical protein binding
IPI
PMID:15629713
Binding of natively unfolded HIF-1alpha ODD domain to p53. 		ACCEPT
Summary: p53 binds to itself to form tetramers through its oligomerization domain, essential for DNA binding.
Reason: Core structural feature - p53 tetramerization through identical protein binding is essential for function.
Supporting Evidence:
PDB:1C26
Crystal structure of p53 tetramerization domain
PMID:10876243
Structure of the negative regulatory domain of p53 bound to S100B(betabeta).
PMID:14759370
Structural mechanism of the bromodomain of the coactivator CBP in p53 transcriptional activation.
PMID:14985081
Hepatitis C virus core protein interacts with p53-binding protein, 53BP2/Bbp/ASPP2, and inhibits p53-mediated apoptosis.
PMID:16291740
2005 Nov 15. Charge modification at multiple C-terminal lysine residues regulates p53 oligomerization and its nucleus-cytoplasm trafficking.
PMID:16461914
Core domain interactions in full-length p53 in solution.
PMID:17612295
Yeast split-ubiquitin-based cytosolic screening system to detect interactions between transcriptionally active proteins.
PMID:17620598
Quaternary structures of tumor suppressor p53 and a specific p53 DNA complex.
PMID:18087040
Regulation of p53 tetramerization and nuclear export by ARC.
PMID:19339993
Crosstalk between sumoylation and acetylation regulates p53-dependent chromatin transcription and DNA binding.
PMID:19667193
Ultraslow oligomerization equilibria of p53 and its implications.
PMID:20004160
Averaging of electron subtomograms and random conical tilt reconstructions through likelihood optimization.
PMID:20159469
Crystal structure of the p53 core domain bound to a full consensus site as a self-assembled tetramer.
PMID:20364130
Apr 4. Diversity in DNA recognition by p53 revealed by crystal structures with Hoogsteen base pairs.
PMID:21178074
Electron microscopy studies on the quaternary structure of p53 reveal different binding modes for p53 tetramers in complex with DNA.
PMID:21522129
An induced fit mechanism regulates p53 DNA binding kinetics to confer sequence specificity.
PMID:21988832
Toward an understanding of the protein interaction network of the human liver.
PMID:22653443
Mutant p53 interactome identifies nardilysin as a p53R273H-specific binding partner that promotes invasion.
PMID:22972749
Domain-domain interactions in full-length p53 and a specific DNA complex probed by methyl NMR spectroscopy.
PMID:25402006
In silico prediction of physical protein interactions and characterization of interactome orphans.
PMID:25609649
Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes.
PMID:31837246
High-throughput competitive fluorescence polarization assay reveals functional redundancy in the S100 protein family.
PMID:35512704
2022 May 4. Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.
PMID:15629713
Binding of natively unfolded HIF-1alpha ODD domain to p53.
PMID:19011621
Arginine methylation regulates the p53 response.
GO:0005515 protein binding
IPI
PMID:28842590
DDX3 localizes to the centrosome and prevents multipolar mit... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:28842590
DDX3 localizes to the centrosome and prevents multipolar mitosis by epigenetically and translationally modulating p53 expression.
GO:0005813 centrosome
IDA
PMID:28842590
DDX3 localizes to the centrosome and prevents multipolar mit... 		KEEP AS NON CORE
Summary: p53 has been reported to localize to centrosomes but this is not a primary localization.
Reason: Non-core localization. p53 may associate with centrosomes in certain contexts but this is not a primary function.
Supporting Evidence:
PMID:28842590
DDX3 localizes to the centrosome and prevents multipolar mitosis by epigenetically and translationally modulating p53 expression.
GO:0042802 identical protein binding
IPI
PMID:19011621
Arginine methylation regulates the p53 response. 		ACCEPT
Summary: p53 binds to itself to form tetramers through its oligomerization domain, essential for DNA binding.
Reason: Core structural feature - p53 tetramerization through identical protein binding is essential for function.
Supporting Evidence:
PDB:1C26
Crystal structure of p53 tetramerization domain
PMID:10876243
Structure of the negative regulatory domain of p53 bound to S100B(betabeta).
PMID:14759370
Structural mechanism of the bromodomain of the coactivator CBP in p53 transcriptional activation.
PMID:14985081
Hepatitis C virus core protein interacts with p53-binding protein, 53BP2/Bbp/ASPP2, and inhibits p53-mediated apoptosis.
PMID:16291740
2005 Nov 15. Charge modification at multiple C-terminal lysine residues regulates p53 oligomerization and its nucleus-cytoplasm trafficking.
PMID:16461914
Core domain interactions in full-length p53 in solution.
PMID:17612295
Yeast split-ubiquitin-based cytosolic screening system to detect interactions between transcriptionally active proteins.
PMID:17620598
Quaternary structures of tumor suppressor p53 and a specific p53 DNA complex.
PMID:18087040
Regulation of p53 tetramerization and nuclear export by ARC.
PMID:19339993
Crosstalk between sumoylation and acetylation regulates p53-dependent chromatin transcription and DNA binding.
PMID:19667193
Ultraslow oligomerization equilibria of p53 and its implications.
PMID:20004160
Averaging of electron subtomograms and random conical tilt reconstructions through likelihood optimization.
PMID:20159469
Crystal structure of the p53 core domain bound to a full consensus site as a self-assembled tetramer.
PMID:20364130
Apr 4. Diversity in DNA recognition by p53 revealed by crystal structures with Hoogsteen base pairs.
PMID:21178074
Electron microscopy studies on the quaternary structure of p53 reveal different binding modes for p53 tetramers in complex with DNA.
PMID:21522129
An induced fit mechanism regulates p53 DNA binding kinetics to confer sequence specificity.
PMID:21988832
Toward an understanding of the protein interaction network of the human liver.
PMID:22653443
Mutant p53 interactome identifies nardilysin as a p53R273H-specific binding partner that promotes invasion.
PMID:22972749
Domain-domain interactions in full-length p53 and a specific DNA complex probed by methyl NMR spectroscopy.
PMID:25402006
In silico prediction of physical protein interactions and characterization of interactome orphans.
PMID:25609649
Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes.
PMID:31837246
High-throughput competitive fluorescence polarization assay reveals functional redundancy in the S100 protein family.
PMID:35512704
2022 May 4. Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.
PMID:15629713
Binding of natively unfolded HIF-1alpha ODD domain to p53.
PMID:19011621
Arginine methylation regulates the p53 response.
GO:0043065 positive regulation of apoptotic process
IDA
PMID:12667443
p53 has a direct apoptogenic role at the mitochondria. 		ACCEPT
Summary: p53 positively regulates apoptosis as a major tumor suppressor mechanism.
Reason: Core function - p53 induces apoptosis through multiple pathways.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression
PMID:12667443
p53 has a direct apoptogenic role at the mitochondria.
PMID:15565177
A novel mitochondrial protein DIP mediates E2F1-induced apoptosis independently of p53.
PMID:20959462
2010 Oct 19. Aurora B interacts with NIR-p53, leading to p53 phosphorylation in its DNA-binding domain and subsequent functional suppression.
GO:0005515 protein binding
IPI
PMID:9733515
Activation of the ATM kinase by ionizing radiation and phosp... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:9733515
Activation of the ATM kinase by ionizing radiation and phosphorylation of p53.
GO:0005515 protein binding
IPI
PMID:16443602
WT p53, but not tumor-derived mutants, bind to Bcl2 via the ... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:16443602
2006 Jan 26. WT p53, but not tumor-derived mutants, bind to Bcl2 via the DNA binding domain and induce mitochondrial permeabilization.
GO:0000785 chromatin
ISA
GO_REF:0000113 		ACCEPT
Summary: p53 associates with chromatin at target gene promoters to regulate transcription.
Reason: Core molecular function - p53 binds to chromatin at p53 response elements to regulate target gene expression.
Supporting Evidence:
PDB:3TS8
Crystal structure of a multidomain human p53 tetramer bound to the natural CDKN1A(p21) p53-response element
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:30089260
p53 Regulates the Expression of LRP1 and Apoptosis through a Stress Intensity-Dependent MicroRNA Feedback Loop.
PMID:23629966
Deacetylation of p53 induces autophagy by suppressing Bmf expression.
PMID:22521434
Hepatocyte growth factor (HGF) and 1,25-dihydroxyvitamin D together stimulate human bone marrow-derived stem cells toward the osteogenic phenotype by HGF-induced up-regulation of VDR.
PMID:15710329
Human MUC1 oncoprotein regulates p53-responsive gene transcription in the genotoxic stress response.
PMID:17805299
p53 is regulated by the lysine demethylase LSD1.
GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific
ISA
GO_REF:0000113 		ACCEPT
Summary: p53 is a DNA-binding transcription factor that regulates RNA polymerase II transcription of target genes.
Reason: Core molecular function - p53 is a well-characterized sequence-specific transcription factor for RNA pol II.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:34381247
Epub 2021 Aug 11. The tumor suppressor folliculin inhibits lactate dehydrogenase A and regulates the Warburg effect.
PMID:35618207
Ser392 phosphorylation modulated a switch between p53 and transcriptional condensates.
PMID:36634798
Phosphorylation and specific DNA improved the incorporation ability of p53 into functional condensates.
PMID:38653238
2024 Apr 22. Alanyl-tRNA synthetase, AARS1, is a lactate sensor and lactyltransferase that lactylates p53 and contributes to tumorigenesis.
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:17310983
Feb 19. Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function.
PMID:24652652
2014 Mar 20. DRAGO (KIAA0247), a new DNA damage-responsive, p53-inducible gene that cooperates with p53 as oncosuppressor.
GO:0002244 hematopoietic progenitor cell differentiation
IMP
PMID:30146126
De Novo Mutations Activating Germline TP53 in an Inherited B... 		KEEP AS NON CORE
Summary: p53 is involved in hematopoietic progenitor cell differentiation but is not essential.
Reason: Non-core developmental function.
Supporting Evidence:
PMID:30146126
Epub 2018 Aug 23. De Novo Mutations Activating Germline TP53 in an Inherited Bone-Marrow-Failure Syndrome.
GO:0060218 hematopoietic stem cell differentiation
IMP
PMID:30146126
De Novo Mutations Activating Germline TP53 in an Inherited B... 		KEEP AS NON CORE
Summary: p53 is involved in hematopoietic stem cell differentiation but is not essential.
Reason: Non-core developmental function.
Supporting Evidence:
PMID:30146126
Epub 2018 Aug 23. De Novo Mutations Activating Germline TP53 in an Inherited Bone-Marrow-Failure Syndrome.
GO:0048539 bone marrow development
IMP
PMID:30146126
De Novo Mutations Activating Germline TP53 in an Inherited B... 		KEEP AS NON CORE
Summary: p53 influences olfactory bulb development but is not essential.
Reason: Non-core developmental function - tissue-specific.
Supporting Evidence:
PMID:30146126
Epub 2018 Aug 23. De Novo Mutations Activating Germline TP53 in an Inherited Bone-Marrow-Failure Syndrome.
GO:0005515 protein binding
IPI
PMID:19011621
Arginine methylation regulates the p53 response. 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:19011621
Arginine methylation regulates the p53 response.
GO:0005634 nucleus
IDA
PMID:19011621
Arginine methylation regulates the p53 response. 		ACCEPT
Summary: p53 is primarily a nuclear transcription factor, with nuclear localization essential for its function.
Reason: Core cellular component - p53's nuclear localization is essential for its transcription factor activity. Contains nuclear localization signals at residues 305-322 and 369-375.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Endoplasmic reticulum.
PMID:14963330
Direct activation of Bax by p53 mediates mitochondrial membrane permeabilization and apoptosis.
PMID:16322561
NIR is a novel INHAT repressor that modulates the transcriptional activity of p53.
PMID:36108750
Phase separation of p53 induced by its unstructured basic region and prevented by oncogenic mutations in tetramerization domain.
PMID:24625977
Ubiquitin-like (UBX)-domain-containing protein, UBXN2A, promotes cell death by interfering with the p53-Mortalin interactions in colon cancer cells.
PMID:26634371
Epub 2015 Dec 4. Structural studies of UBXN2A and mortalin interaction and the putative role of silenced UBXN2A in preventing response to chemotherapy.
PMID:21597459
2011 May 20. E3 ubiquitin ligase Hades negatively regulates the exonuclear function of p53.
PMID:19011621
Arginine methylation regulates the p53 response.
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:24101517
Mitochondrial disulfide relay mediates translocation of p53 and partitions its subcellular activity.
PMID:20810912
Hypoxia downregulates p53 but induces apoptosis and enhances expression of BAD in cultures of human syncytiotrophoblasts.
PMID:19234109
Induction of SOX4 by DNA damage is critical for p53 stabilization and function.
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:17403783
HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53.
PMID:20096447
USP10 regulates p53 localization and stability by deubiquitinating p53.
PMID:18756595
Prognostic significance of BMP and activin membrane-bound inhibitor in colorectal cancer.
PMID:16507995
Myosin VI is a mediator of the p53-dependent cell survival pathway.
PMID:7720704
Direct involvement of p53 in programmed cell death of oligodendrocytes.
GO:0005737 cytoplasm
IDA
PMID:19011621
Arginine methylation regulates the p53 response. 		ACCEPT
Summary: p53 localizes to cytoplasm as well as nucleus, with cytoplasmic localization important for regulation.
Reason: Core cellular component - p53 shuttles between nucleus and cytoplasm, with cytoplasmic localization important for MDM2-mediated regulation.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus. Cytoplasm.
PMID:24625977
Ubiquitin-like (UBX)-domain-containing protein, UBXN2A, promotes cell death by interfering with the p53-Mortalin interactions in colon cancer cells.
PMID:26634371
Epub 2015 Dec 4. Structural studies of UBXN2A and mortalin interaction and the putative role of silenced UBXN2A in preventing response to chemotherapy.
PMID:21597459
2011 May 20. E3 ubiquitin ligase Hades negatively regulates the exonuclear function of p53.
PMID:19011621
Arginine methylation regulates the p53 response.
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:23629966
Deacetylation of p53 induces autophagy by suppressing Bmf expression.
PMID:20810912
Hypoxia downregulates p53 but induces apoptosis and enhances expression of BAD in cultures of human syncytiotrophoblasts.
PMID:16131611
p53 isoforms can regulate p53 transcriptional activity.
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:20096447
USP10 regulates p53 localization and stability by deubiquitinating p53.
PMID:7720704
Direct involvement of p53 in programmed cell death of oligodendrocytes.
GO:2001244 positive regulation of intrinsic apoptotic signaling pathway
ISS
GO_REF:0000024 		ACCEPT
Summary: p53 negatively regulates intrinsic apoptotic signaling in certain contexts.
Reason: p53 can have context-dependent anti-apoptotic effects through p21-mediated survival.
GO:0005515 protein binding
IPI
PMID:18690848
NUPR1 interacts with p53, transcriptionally regulates p21 an... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:18690848
NUPR1 interacts with p53, transcriptionally regulates p21 and rescues breast epithelial cells from doxorubicin-induced genotoxic stress.
GO:2001244 positive regulation of intrinsic apoptotic signaling pathway
IMP
PMID:27031958
Nupr1/Chop signal axis is involved in mitochondrion-related ... 		ACCEPT
Summary: p53 negatively regulates intrinsic apoptotic signaling in certain contexts.
Reason: p53 can have context-dependent anti-apoptotic effects through p21-mediated survival.
Supporting Evidence:
PMID:27031958
Nupr1/Chop signal axis is involved in mitochondrion-related endothelial cell apoptosis induced by methamphetamine.
GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific
IDA
PMID:17146433
RGC32, a novel p53-inducible gene, is located on centrosomes... 		ACCEPT
Summary: p53 activates transcription through binding to promoter sequences.
Reason: Core molecular function - p53 is a transcriptional activator that binds to p53 response elements.
Supporting Evidence:
TP53-deep-research
TP53 is fundamentally a transcription factor that binds sequence-specifically to p53 response elements
PMID:22578566
Apr 19. Oncosuppressive role of p53-induced miR-205 in triple negative breast cancer.
PMID:17145718
Brn-3b enhances the pro-apoptotic effects of p53 but not its induction of cell cycle arrest by cooperating in trans-activation of bax expression.
PMID:12609999
2003 Feb 27. The activation domains, the proline-rich domain, and the C-terminal basic domain in p53 are necessary for acetylation of histones on the proximal p21 promoter and interaction with p300/CREB-binding protein.
PMID:17146433
Dec 4. RGC32, a novel p53-inducible gene, is located on centrosomes during mitosis and results in G2/M arrest.
GO:1990841 promoter-specific chromatin binding
IDA
PMID:20725088
Primate-specific RFPL1 gene controls cell-cycle progression ... 		ACCEPT
Summary: p53 binds to promoter-specific chromatin regions containing p53 response elements.
Reason: Core molecular function - p53 specifically binds to promoter regions of target genes through sequence-specific DNA binding.
Supporting Evidence:
PDB:3TS8
Crystal structure shows p53 binding to CDKN1A(p21) promoter response element
PMID:24652652
2014 Mar 20. DRAGO (KIAA0247), a new DNA damage-responsive, p53-inducible gene that cooperates with p53 as oncosuppressor.
PMID:20725088
2010 Aug 20. Primate-specific RFPL1 gene controls cell-cycle progression through cyclin B1/Cdc2 degradation.
PMID:24356969
2013 Dec 19. Rbm24, an RNA-binding protein and a target of p53, regulates p21 expression via mRNA stability.
GO:0005667 transcription regulator complex
IGI
PMID:17145718
Brn-3b enhances the pro-apoptotic effects of p53 but not its... 		ACCEPT
Summary: p53 is a component of transcription factor complexes.
Reason: Core localization - p53 functions as part of transcriptional machinery complexes.
Supporting Evidence:
TP53-deep-research
Complex formation with transcriptional co-activators (CBP/p300, TFIID)
PMID:17145718
Brn-3b enhances the pro-apoptotic effects of p53 but not its induction of cell cycle arrest by cooperating in trans-activation of bax expression.
GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding
ISS
GO_REF:0000024 		ACCEPT
Summary: p53 binds sequence-specifically to p53 response elements in cis-regulatory regions of target genes.
Reason: Core molecular function - p53's DNA-binding domain binds to consensus p53 response elements (p53REs) with high specificity.
Supporting Evidence:
TP53-deep-research
TP53 is fundamentally a transcription factor that binds sequence-specifically to p53 response elements (p53REs) in target gene promoters
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:22578566
Apr 19. Oncosuppressive role of p53-induced miR-205 in triple negative breast cancer.
GO:1905856 negative regulation of pentose-phosphate shunt
IMP
PMID:21336310
p53 regulates biosynthesis through direct inactivation of gl... 		ACCEPT
Summary: p53 positively regulates p53-mediated DNA damage responses.
Reason: Core function - p53 regulates its own signaling pathway with positive feedback.
Supporting Evidence:
PMID:21336310
p53 regulates biosynthesis through direct inactivation of glucose-6-phosphate dehydrogenase.
GO:0006974 DNA damage response
IMP
PMID:24356969
Rbm24, an RNA-binding protein and a target of p53, regulates... 		ACCEPT
Summary: p53 is central to the DNA damage response, being activated by and coordinating responses to DNA damage.
Reason: Core function - p53 is the 'guardian of the genome' that coordinates DNA damage responses.
Supporting Evidence:
TP53-deep-research
p53 serves as a central node in the DNA damage response network
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:30089260
p53 Regulates the Expression of LRP1 and Apoptosis through a Stress Intensity-Dependent MicroRNA Feedback Loop.
PMID:24356969
2013 Dec 19. Rbm24, an RNA-binding protein and a target of p53, regulates p21 expression via mRNA stability.
PMID:17938203
Oct 15. Protein kinase C delta induces transcription of the TP53 tumor suppressor gene by controlling death-promoting factor Btf in the apoptotic response to DNA damage.
PMID:15710329
Human MUC1 oncoprotein regulates p53-responsive gene transcription in the genotoxic stress response.
GO:0045944 positive regulation of transcription by RNA polymerase II
IMP
PMID:24356969
Rbm24, an RNA-binding protein and a target of p53, regulates... 		ACCEPT
Summary: p53 is a sequence-specific transcriptional activator that upregulates target genes including p21, PUMA, BAX.
Reason: Fundamental molecular function - p53 activates transcription of numerous target genes through binding to p53 response elements.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:16061649
Ckap2 regulates aneuploidy, cell cycling, and cell death in a p53-dependent manner.
PMID:17599062
Jun 28. CDIP, a novel pro-apoptotic gene, regulates TNFalpha-mediated apoptosis in a p53-dependent manner.
PMID:30089260
p53 Regulates the Expression of LRP1 and Apoptosis through a Stress Intensity-Dependent MicroRNA Feedback Loop.
PMID:7958916
DNA damage triggers a prolonged p53-dependent G1 arrest and long-term induction of Cip1 in normal human fibroblasts.
PMID:11672523
hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase.
PMID:17145718
Brn-3b enhances the pro-apoptotic effects of p53 but not its induction of cell cycle arrest by cooperating in trans-activation of bax expression.
PMID:17146433
Dec 4. RGC32, a novel p53-inducible gene, is located on centrosomes during mitosis and results in G2/M arrest.
PMID:18549481
TATA binding protein associated factor 3 (TAF3) interacts with p53 and inhibits its function.
PMID:24356969
2013 Dec 19. Rbm24, an RNA-binding protein and a target of p53, regulates p21 expression via mRNA stability.
PMID:17310983
Feb 19. Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function.
PMID:24652652
2014 Mar 20. DRAGO (KIAA0247), a new DNA damage-responsive, p53-inducible gene that cooperates with p53 as oncosuppressor.
PMID:20959462
2010 Oct 19. Aurora B interacts with NIR-p53, leading to p53 phosphorylation in its DNA-binding domain and subsequent functional suppression.
GO:1990841 promoter-specific chromatin binding
IDA
PMID:24356969
Rbm24, an RNA-binding protein and a target of p53, regulates... 		ACCEPT
Summary: p53 binds to promoter-specific chromatin regions containing p53 response elements.
Reason: Core molecular function - p53 specifically binds to promoter regions of target genes through sequence-specific DNA binding.
Supporting Evidence:
PDB:3TS8
Crystal structure shows p53 binding to CDKN1A(p21) promoter response element
PMID:24652652
2014 Mar 20. DRAGO (KIAA0247), a new DNA damage-responsive, p53-inducible gene that cooperates with p53 as oncosuppressor.
PMID:20725088
2010 Aug 20. Primate-specific RFPL1 gene controls cell-cycle progression through cyclin B1/Cdc2 degradation.
PMID:24356969
2013 Dec 19. Rbm24, an RNA-binding protein and a target of p53, regulates p21 expression via mRNA stability.
GO:0001094 TFIID-class transcription factor complex binding
IPI
PMID:24289924
Phosphorylation of p53 by TAF1 inactivates p53-dependent tra... 		ACCEPT
Summary: p53 interacts with TFIID to regulate transcription initiation.
Reason: Core transcriptional mechanism - p53 recruits TFIID to target promoters.
Supporting Evidence:
TP53-deep-research
Complex formation with TFIID
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
GO:0005634 nucleus
IMP
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negat... 		ACCEPT
Summary: p53 is primarily a nuclear transcription factor, with nuclear localization essential for its function.
Reason: Core cellular component - p53's nuclear localization is essential for its transcription factor activity. Contains nuclear localization signals at residues 305-322 and 369-375.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Endoplasmic reticulum.
PMID:14963330
Direct activation of Bax by p53 mediates mitochondrial membrane permeabilization and apoptosis.
PMID:16322561
NIR is a novel INHAT repressor that modulates the transcriptional activity of p53.
PMID:36108750
Phase separation of p53 induced by its unstructured basic region and prevented by oncogenic mutations in tetramerization domain.
PMID:24625977
Ubiquitin-like (UBX)-domain-containing protein, UBXN2A, promotes cell death by interfering with the p53-Mortalin interactions in colon cancer cells.
PMID:26634371
Epub 2015 Dec 4. Structural studies of UBXN2A and mortalin interaction and the putative role of silenced UBXN2A in preventing response to chemotherapy.
PMID:21597459
2011 May 20. E3 ubiquitin ligase Hades negatively regulates the exonuclear function of p53.
PMID:19011621
Arginine methylation regulates the p53 response.
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:24101517
Mitochondrial disulfide relay mediates translocation of p53 and partitions its subcellular activity.
PMID:20810912
Hypoxia downregulates p53 but induces apoptosis and enhances expression of BAD in cultures of human syncytiotrophoblasts.
PMID:19234109
Induction of SOX4 by DNA damage is critical for p53 stabilization and function.
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:17403783
HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53.
PMID:20096447
USP10 regulates p53 localization and stability by deubiquitinating p53.
PMID:18756595
Prognostic significance of BMP and activin membrane-bound inhibitor in colorectal cancer.
PMID:16507995
Myosin VI is a mediator of the p53-dependent cell survival pathway.
PMID:7720704
Direct involvement of p53 in programmed cell death of oligodendrocytes.
GO:0005737 cytoplasm
IMP
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negat... 		ACCEPT
Summary: p53 localizes to cytoplasm as well as nucleus, with cytoplasmic localization important for regulation.
Reason: Core cellular component - p53 shuttles between nucleus and cytoplasm, with cytoplasmic localization important for MDM2-mediated regulation.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus. Cytoplasm.
PMID:24625977
Ubiquitin-like (UBX)-domain-containing protein, UBXN2A, promotes cell death by interfering with the p53-Mortalin interactions in colon cancer cells.
PMID:26634371
Epub 2015 Dec 4. Structural studies of UBXN2A and mortalin interaction and the putative role of silenced UBXN2A in preventing response to chemotherapy.
PMID:21597459
2011 May 20. E3 ubiquitin ligase Hades negatively regulates the exonuclear function of p53.
PMID:19011621
Arginine methylation regulates the p53 response.
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:23629966
Deacetylation of p53 induces autophagy by suppressing Bmf expression.
PMID:20810912
Hypoxia downregulates p53 but induces apoptosis and enhances expression of BAD in cultures of human syncytiotrophoblasts.
PMID:16131611
p53 isoforms can regulate p53 transcriptional activity.
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:20096447
USP10 regulates p53 localization and stability by deubiquitinating p53.
PMID:7720704
Direct involvement of p53 in programmed cell death of oligodendrocytes.
GO:0009299 mRNA transcription
IMP
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negat... 		KEEP AS NON CORE
Summary: p53 influences mRNA processing but this is not a core function.
Reason: Non-core regulatory function.
Supporting Evidence:
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
GO:0030330 DNA damage response, signal transduction by p53 class mediator
IMP
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negat... 		ACCEPT
Summary: p53 mediates DNA damage response signal transduction as THE p53 class mediator.
Reason: Absolutely core function - p53 is the defining member of the p53 class mediator family.
Supporting Evidence:
TP53-deep-research
p53 serves as a central node in the DNA damage response network
PMID:29681526
A Designed Peptide Targets Two Types of Modifications of p53 with Anti-cancer Activity.
PMID:15149599
Telomere shortening triggers senescence of human cells through a pathway involving ATM, p53, and p21(CIP1), but not p16(INK4a).
PMID:20160708
Feedback between p21 and reactive oxygen production is necessary for cell senescence.
PMID:7958916
DNA damage triggers a prolonged p53-dependent G1 arrest and long-term induction of Cip1 in normal human fibroblasts.
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:16213212
Regulation of p53 translation and induction after DNA damage by ribosomal protein L26 and nucleolin.
GO:0043065 positive regulation of apoptotic process
IDA
PMID:15565177
A novel mitochondrial protein DIP mediates E2F1-induced apop... 		ACCEPT
Summary: p53 positively regulates apoptosis as a major tumor suppressor mechanism.
Reason: Core function - p53 induces apoptosis through multiple pathways.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression
PMID:12667443
p53 has a direct apoptogenic role at the mitochondria.
PMID:15565177
A novel mitochondrial protein DIP mediates E2F1-induced apoptosis independently of p53.
PMID:20959462
2010 Oct 19. Aurora B interacts with NIR-p53, leading to p53 phosphorylation in its DNA-binding domain and subsequent functional suppression.
GO:0005515 protein binding
IPI
PMID:17904127
SVH-B interacts directly with p53 and suppresses the transcr... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:17904127
Epub 2007 Sep 21. SVH-B interacts directly with p53 and suppresses the transcriptional activity of p53.
GO:0005515 protein binding
IPI
PMID:25422469
Disruption of FAT10-MAD2 binding inhibits tumor progression. 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:25422469
Disruption of FAT10-MAD2 binding inhibits tumor progression.
GO:0005515 protein binding
IPI
PMID:27323408
LACE1 interacts with p53 and mediates its mitochondrial tran... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:27323408
LACE1 interacts with p53 and mediates its mitochondrial translocation and apoptosis.
GO:0005515 protein binding
IPI
PMID:22522597
Nucleolar protein GLTSCR2 stabilizes p53 in response to ribo... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:22522597
2012 Apr 20. Nucleolar protein GLTSCR2 stabilizes p53 in response to ribosomal stresses.
GO:0003677 DNA binding
IDA
PMID:15358771
Hsp90 regulates the activity of wild type p53 under physiolo... 		ACCEPT
Summary: p53 binds DNA through its central DNA-binding domain (residues 102-292) to p53 response elements.
Reason: Core molecular function - DNA binding is essential for p53's transcription factor activity.
Supporting Evidence:
TP53-deep-research
DNA-Binding Domain (DBD): Location: Residues 102-292; Structure: beta-sandwich core with DNA-binding loops
PMID:15358771
2004 Sep 9. Hsp90 regulates the activity of wild type p53 under physiological and elevated temperatures.
PMID:15629713
Binding of natively unfolded HIF-1alpha ODD domain to p53.
PMID:2144364
Transcriptional activation by wild-type but not transforming mutants of the p53 anti-oncogene.
GO:0051087 protein-folding chaperone binding
IPI
PMID:15358771
Hsp90 regulates the activity of wild type p53 under physiolo... 		KEEP AS NON CORE
Summary: p53 has chaperone binding activity related to its folding and stability.
Reason: Non-core function related to protein folding and quality control.
Supporting Evidence:
PMID:15358771
2004 Sep 9. Hsp90 regulates the activity of wild type p53 under physiological and elevated temperatures.
GO:0005515 protein binding
IPI
PMID:17145718
Brn-3b enhances the pro-apoptotic effects of p53 but not its... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:17145718
Brn-3b enhances the pro-apoptotic effects of p53 but not its induction of cell cycle arrest by cooperating in trans-activation of bax expression.
GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific
IDA
PMID:17310983
Ribosomal protein S7 as a novel modulator of p53-MDM2 intera... 		ACCEPT
Summary: p53 is a DNA-binding transcription factor that regulates RNA polymerase II transcription of target genes.
Reason: Core molecular function - p53 is a well-characterized sequence-specific transcription factor for RNA pol II.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:34381247
Epub 2021 Aug 11. The tumor suppressor folliculin inhibits lactate dehydrogenase A and regulates the Warburg effect.
PMID:35618207
Ser392 phosphorylation modulated a switch between p53 and transcriptional condensates.
PMID:36634798
Phosphorylation and specific DNA improved the incorporation ability of p53 into functional condensates.
PMID:38653238
2024 Apr 22. Alanyl-tRNA synthetase, AARS1, is a lactate sensor and lactyltransferase that lactylates p53 and contributes to tumorigenesis.
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:17310983
Feb 19. Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function.
PMID:24652652
2014 Mar 20. DRAGO (KIAA0247), a new DNA damage-responsive, p53-inducible gene that cooperates with p53 as oncosuppressor.
GO:0003730 mRNA 3'-UTR binding
IDA
PMID:16213212
Regulation of p53 translation and induction after DNA damage... 		ACCEPT
Summary: p53 binds mRNA and regulates gene expression post-transcriptionally.
Reason: p53 has RNA-binding activity that contributes to gene regulation.
Supporting Evidence:
PMID:16213212
Regulation of p53 translation and induction after DNA damage by ribosomal protein L26 and nucleolin.
GO:0010628 positive regulation of gene expression
IDA
PMID:15314173
Ribosomal protein L23 activates p53 by inhibiting MDM2 funct... 		ACCEPT
Summary: p53 positively regulates gene expression of numerous target genes.
Reason: Core transcriptional function - p53 is a transcriptional activator.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:26100857
A metabolic stress-inducible miR-34a-HNF4Ξ± pathway regulates lipid and lipoprotein metabolism.
PMID:20332243
2010 Mar 23. MicroRNA145 targets BNIP3 and suppresses prostate cancer progression.
PMID:15314173
Ribosomal protein L23 activates p53 by inhibiting MDM2 function in response to ribosomal perturbation but not to translation inhibition.
PMID:19160485
A ribosomal protein L23-nucleophosmin circuit coordinates Mizl function with cell growth.
GO:0010628 positive regulation of gene expression
IMP
PMID:19160485
A ribosomal protein L23-nucleophosmin circuit coordinates Mi... 		ACCEPT
Summary: p53 positively regulates gene expression of numerous target genes.
Reason: Core transcriptional function - p53 is a transcriptional activator.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:26100857
A metabolic stress-inducible miR-34a-HNF4Ξ± pathway regulates lipid and lipoprotein metabolism.
PMID:20332243
2010 Mar 23. MicroRNA145 targets BNIP3 and suppresses prostate cancer progression.
PMID:15314173
Ribosomal protein L23 activates p53 by inhibiting MDM2 function in response to ribosomal perturbation but not to translation inhibition.
PMID:19160485
A ribosomal protein L23-nucleophosmin circuit coordinates Mizl function with cell growth.
GO:0030330 DNA damage response, signal transduction by p53 class mediator
IDA
PMID:16213212
Regulation of p53 translation and induction after DNA damage... 		ACCEPT
Summary: p53 mediates DNA damage response signal transduction as THE p53 class mediator.
Reason: Absolutely core function - p53 is the defining member of the p53 class mediator family.
Supporting Evidence:
TP53-deep-research
p53 serves as a central node in the DNA damage response network
PMID:29681526
A Designed Peptide Targets Two Types of Modifications of p53 with Anti-cancer Activity.
PMID:15149599
Telomere shortening triggers senescence of human cells through a pathway involving ATM, p53, and p21(CIP1), but not p16(INK4a).
PMID:20160708
Feedback between p21 and reactive oxygen production is necessary for cell senescence.
PMID:7958916
DNA damage triggers a prolonged p53-dependent G1 arrest and long-term induction of Cip1 in normal human fibroblasts.
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:16213212
Regulation of p53 translation and induction after DNA damage by ribosomal protein L26 and nucleolin.
GO:0030330 DNA damage response, signal transduction by p53 class mediator
IMP
PMID:16213212
Regulation of p53 translation and induction after DNA damage... 		ACCEPT
Summary: p53 mediates DNA damage response signal transduction as THE p53 class mediator.
Reason: Absolutely core function - p53 is the defining member of the p53 class mediator family.
Supporting Evidence:
TP53-deep-research
p53 serves as a central node in the DNA damage response network
PMID:29681526
A Designed Peptide Targets Two Types of Modifications of p53 with Anti-cancer Activity.
PMID:15149599
Telomere shortening triggers senescence of human cells through a pathway involving ATM, p53, and p21(CIP1), but not p16(INK4a).
PMID:20160708
Feedback between p21 and reactive oxygen production is necessary for cell senescence.
PMID:7958916
DNA damage triggers a prolonged p53-dependent G1 arrest and long-term induction of Cip1 in normal human fibroblasts.
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:16213212
Regulation of p53 translation and induction after DNA damage by ribosomal protein L26 and nucleolin.
GO:0032991 protein-containing complex
IDA
PMID:17310983
Ribosomal protein S7 as a novel modulator of p53-MDM2 intera... 		ACCEPT
Summary: p53 forms part of protein-containing complexes including the p53 tetramer and complexes with co-activators.
Reason: p53 functions in complexes with CBP/p300, MDM2, and forms tetramers.
Supporting Evidence:
TP53-deep-research
Complex formation with transcriptional co-activators (CBP/p300, TFIID)
PMID:17310983
Feb 19. Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function.
PMID:15629713
Binding of natively unfolded HIF-1alpha ODD domain to p53.
PMID:9529249
ARF promotes MDM2 degradation and stabilizes p53: ARF-INK4a locus deletion impairs both the Rb and p53 tumor suppression pathways.
GO:0034644 cellular response to UV
IDA
PMID:16213212
Regulation of p53 translation and induction after DNA damage... 		ACCEPT
Summary: p53 responds to UV-induced DNA damage as part of its DNA damage response.
Reason: Core function - UV radiation is a classic p53-activating stress.
Supporting Evidence:
GO:0009411
response to UV
PMID:16213212
Regulation of p53 translation and induction after DNA damage by ribosomal protein L26 and nucleolin.
GO:0042771 intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
IMP
PMID:16213212
Regulation of p53 translation and induction after DNA damage... 		ACCEPT
Summary: p53 is THE p53 class mediator that induces intrinsic apoptosis in response to DNA damage through BAX, PUMA, NOXA activation.
Reason: Absolutely core function - this term specifically describes p53's role as the central mediator of DNA damage-induced apoptosis.
Supporting Evidence:
TP53-deep-research
Transcriptional: Activation of pro-apoptotic genes (PUMA, BAX, NOXA, FAS)
PMID:14654789
A member of the Pyrin family, IFI16, is a novel BRCA1-associated protein involved in the p53-mediated apoptosis pathway.
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:16213212
Regulation of p53 translation and induction after DNA damage by ribosomal protein L26 and nucleolin.
PMID:17403783
HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53.
GO:0045944 positive regulation of transcription by RNA polymerase II
IDA
PMID:17310983
Ribosomal protein S7 as a novel modulator of p53-MDM2 intera... 		ACCEPT
Summary: p53 is a sequence-specific transcriptional activator that upregulates target genes including p21, PUMA, BAX.
Reason: Fundamental molecular function - p53 activates transcription of numerous target genes through binding to p53 response elements.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:16061649
Ckap2 regulates aneuploidy, cell cycling, and cell death in a p53-dependent manner.
PMID:17599062
Jun 28. CDIP, a novel pro-apoptotic gene, regulates TNFalpha-mediated apoptosis in a p53-dependent manner.
PMID:30089260
p53 Regulates the Expression of LRP1 and Apoptosis through a Stress Intensity-Dependent MicroRNA Feedback Loop.
PMID:7958916
DNA damage triggers a prolonged p53-dependent G1 arrest and long-term induction of Cip1 in normal human fibroblasts.
PMID:11672523
hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase.
PMID:17145718
Brn-3b enhances the pro-apoptotic effects of p53 but not its induction of cell cycle arrest by cooperating in trans-activation of bax expression.
PMID:17146433
Dec 4. RGC32, a novel p53-inducible gene, is located on centrosomes during mitosis and results in G2/M arrest.
PMID:18549481
TATA binding protein associated factor 3 (TAF3) interacts with p53 and inhibits its function.
PMID:24356969
2013 Dec 19. Rbm24, an RNA-binding protein and a target of p53, regulates p21 expression via mRNA stability.
PMID:17310983
Feb 19. Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function.
PMID:24652652
2014 Mar 20. DRAGO (KIAA0247), a new DNA damage-responsive, p53-inducible gene that cooperates with p53 as oncosuppressor.
PMID:20959462
2010 Oct 19. Aurora B interacts with NIR-p53, leading to p53 phosphorylation in its DNA-binding domain and subsequent functional suppression.
GO:0071480 cellular response to gamma radiation
IDA
PMID:16213212
Regulation of p53 translation and induction after DNA damage... 		ACCEPT
Summary: p53 responds to gamma radiation-induced DNA damage.
Reason: Core function - ionizing radiation is a classic p53 activator.
Supporting Evidence:
TP53-deep-research
gamma radiation causes DNA damage that strongly activates p53
PMID:16213212
Regulation of p53 translation and induction after DNA damage by ribosomal protein L26 and nucleolin.
GO:0072331 signal transduction by p53 class mediator
IDA
PMID:15314173
Ribosomal protein L23 activates p53 by inhibiting MDM2 funct... 		ACCEPT
Summary: p53 IS the p53 class mediator that transduces signals in response to stress.
Reason: Absolutely core function - this term specifically describes p53's central role in stress signaling.
Supporting Evidence:
TP53-deep-research
p53 coordinates cellular responses to diverse stress signals
PMID:25384516
Rad54B serves as a scaffold in the DNA damage response that limits checkpoint strength.
PMID:18549481
TATA binding protein associated factor 3 (TAF3) interacts with p53 and inhibits its function.
PMID:29681526
A Designed Peptide Targets Two Types of Modifications of p53 with Anti-cancer Activity.
PMID:15314173
Ribosomal protein L23 activates p53 by inhibiting MDM2 function in response to ribosomal perturbation but not to translation inhibition.
GO:0072332 intrinsic apoptotic signaling pathway by p53 class mediator
IMP
PMID:17310983
Ribosomal protein S7 as a novel modulator of p53-MDM2 intera... 		ACCEPT
Summary: p53 mediates intrinsic apoptotic signaling through BAX, PUMA, NOXA activation.
Reason: Core apoptotic function - p53 is the master regulator of intrinsic apoptosis.
Supporting Evidence:
TP53-deep-research
Transcriptional: Activation of pro-apoptotic genes (PUMA, BAX, NOXA)
PMID:17310983
Feb 19. Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function.
PMID:16322561
NIR is a novel INHAT repressor that modulates the transcriptional activity of p53.
PMID:12172011
Chk2 regulates irradiation-induced, p53-mediated apoptosis in Drosophila.
GO:0072717 cellular response to actinomycin D
IDA
PMID:15314173
Ribosomal protein L23 activates p53 by inhibiting MDM2 funct... 		ACCEPT
Summary: p53 mediates intrinsic apoptotic signaling in response to DNA damage.
Reason: Core function - DNA damage-induced apoptosis is a major p53 mechanism.
Supporting Evidence:
GO:0042771
intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
PMID:15314173
Ribosomal protein L23 activates p53 by inhibiting MDM2 function in response to ribosomal perturbation but not to translation inhibition.
GO:0005515 protein binding
IPI
PMID:25591766
RNF125 is a ubiquitin-protein ligase that promotes p53 degra... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:25591766
RNF125 is a ubiquitin-protein ligase that promotes p53 degradation.
GO:0002039 p53 binding
IPI
PMID:25417702
Functional interplay between MDM2, p63/p73 and mutant p53. 		ACCEPT
Summary: p53 is a substrate of p21 (CDKN1A), creating a regulatory feedback loop.
Reason: Regulatory interaction - p21 is both a target and regulator of p53.
Supporting Evidence:
PMID:25417702
Functional interplay between MDM2, p63/p73 and mutant p53.
GO:0006355 regulation of DNA-templated transcription
IMP
PMID:25417702
Functional interplay between MDM2, p63/p73 and mutant p53. 		ACCEPT
Summary: p53 regulates DNA-templated transcription as both an activator and repressor.
Reason: Core molecular function - transcription regulation is fundamental to p53's tumor suppressor activity.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:25417702
Functional interplay between MDM2, p63/p73 and mutant p53.
PMID:7587074
Targets for transcriptional activation by wild-type p53: endogenous retroviral LTR, immunoglobulin-like promoter, and an internal promoter of the mdm2 gene.
GO:0003677 DNA binding
IMP
PMID:15629713
Binding of natively unfolded HIF-1alpha ODD domain to p53. 		ACCEPT
Summary: p53 binds DNA through its central DNA-binding domain (residues 102-292) to p53 response elements.
Reason: Core molecular function - DNA binding is essential for p53's transcription factor activity.
Supporting Evidence:
TP53-deep-research
DNA-Binding Domain (DBD): Location: Residues 102-292; Structure: beta-sandwich core with DNA-binding loops
PMID:15358771
2004 Sep 9. Hsp90 regulates the activity of wild type p53 under physiological and elevated temperatures.
PMID:15629713
Binding of natively unfolded HIF-1alpha ODD domain to p53.
PMID:2144364
Transcriptional activation by wild-type but not transforming mutants of the p53 anti-oncogene.
GO:0032991 protein-containing complex
IMP
PMID:15629713
Binding of natively unfolded HIF-1alpha ODD domain to p53. 		ACCEPT
Summary: p53 forms part of protein-containing complexes including the p53 tetramer and complexes with co-activators.
Reason: p53 functions in complexes with CBP/p300, MDM2, and forms tetramers.
Supporting Evidence:
TP53-deep-research
Complex formation with transcriptional co-activators (CBP/p300, TFIID)
PMID:17310983
Feb 19. Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function.
PMID:15629713
Binding of natively unfolded HIF-1alpha ODD domain to p53.
PMID:9529249
ARF promotes MDM2 degradation and stabilizes p53: ARF-INK4a locus deletion impairs both the Rb and p53 tumor suppression pathways.
GO:0097718 disordered domain specific binding
IPI
PMID:15629713
Binding of natively unfolded HIF-1alpha ODD domain to p53. 		KEEP AS NON CORE
Summary: p53 is involved in dimerization of proteins.
Reason: Non-core - while p53 dimerizes, this is covered by more specific tetramerization terms.
Supporting Evidence:
PMID:15629713
Binding of natively unfolded HIF-1alpha ODD domain to p53.
GO:0000785 chromatin
IDA
PMID:23629966
Deacetylation of p53 induces autophagy by suppressing Bmf ex... 		ACCEPT
Summary: p53 associates with chromatin at target gene promoters to regulate transcription.
Reason: Core molecular function - p53 binds to chromatin at p53 response elements to regulate target gene expression.
Supporting Evidence:
PDB:3TS8
Crystal structure of a multidomain human p53 tetramer bound to the natural CDKN1A(p21) p53-response element
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:30089260
p53 Regulates the Expression of LRP1 and Apoptosis through a Stress Intensity-Dependent MicroRNA Feedback Loop.
PMID:23629966
Deacetylation of p53 induces autophagy by suppressing Bmf expression.
PMID:22521434
Hepatocyte growth factor (HGF) and 1,25-dihydroxyvitamin D together stimulate human bone marrow-derived stem cells toward the osteogenic phenotype by HGF-induced up-regulation of VDR.
PMID:15710329
Human MUC1 oncoprotein regulates p53-responsive gene transcription in the genotoxic stress response.
PMID:17805299
p53 is regulated by the lysine demethylase LSD1.
GO:0005737 cytoplasm
IDA
PMID:23629966
Deacetylation of p53 induces autophagy by suppressing Bmf ex... 		ACCEPT
Summary: p53 localizes to cytoplasm as well as nucleus, with cytoplasmic localization important for regulation.
Reason: Core cellular component - p53 shuttles between nucleus and cytoplasm, with cytoplasmic localization important for MDM2-mediated regulation.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus. Cytoplasm.
PMID:24625977
Ubiquitin-like (UBX)-domain-containing protein, UBXN2A, promotes cell death by interfering with the p53-Mortalin interactions in colon cancer cells.
PMID:26634371
Epub 2015 Dec 4. Structural studies of UBXN2A and mortalin interaction and the putative role of silenced UBXN2A in preventing response to chemotherapy.
PMID:21597459
2011 May 20. E3 ubiquitin ligase Hades negatively regulates the exonuclear function of p53.
PMID:19011621
Arginine methylation regulates the p53 response.
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:23629966
Deacetylation of p53 induces autophagy by suppressing Bmf expression.
PMID:20810912
Hypoxia downregulates p53 but induces apoptosis and enhances expression of BAD in cultures of human syncytiotrophoblasts.
PMID:16131611
p53 isoforms can regulate p53 transcriptional activity.
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:20096447
USP10 regulates p53 localization and stability by deubiquitinating p53.
PMID:7720704
Direct involvement of p53 in programmed cell death of oligodendrocytes.
GO:0006914 autophagy
IMP
PMID:23629966
Deacetylation of p53 induces autophagy by suppressing Bmf ex... 		ACCEPT
Summary: p53 regulates autophagy both positively and negatively depending on context.
Reason: p53 regulates autophagy as part of cellular stress response.
Supporting Evidence:
TP53-deep-research
p53 also regulates autophagy
PMID:23629966
Deacetylation of p53 induces autophagy by suppressing Bmf expression.
GO:0042826 histone deacetylase binding
IPI
PMID:23629966
Deacetylation of p53 induces autophagy by suppressing Bmf ex... 		ACCEPT
Summary: p53 undergoes deacetylation as part of its regulatory cycle.
Reason: Core regulatory mechanism - p53 acetylation/deacetylation cycle regulates its activity.
Supporting Evidence:
TP53-deep-research
C-terminal acetylation regulates p53 activity
PMID:23629966
Deacetylation of p53 induces autophagy by suppressing Bmf expression.
GO:0045892 negative regulation of DNA-templated transcription
IMP
PMID:23629966
Deacetylation of p53 induces autophagy by suppressing Bmf ex... 		ACCEPT
Summary: p53 negatively regulates transcription of various target genes including anti-apoptotic and cell cycle genes.
Reason: Core function - p53 acts as both a transcriptional activator and repressor.
Supporting Evidence:
TP53-deep-research
Repression: Downregulates anti-apoptotic genes (BCL2), cell cycle progression genes
PMID:16492744
Mdm4 and Mdm2 cooperate to inhibit p53 activity in proliferating and quiescent cells in vivo.
PMID:9271120
Repression of p53-mediated transcription by MDM2: a dual mechanism.
PMID:23629966
Deacetylation of p53 induces autophagy by suppressing Bmf expression.
PMID:24051492
p53 regulates Period2 expression and the circadian clock.
GO:0005515 protein binding
IPI
PMID:17237821
Transcription factor TAFII250 promotes Mdm2-dependent turnov... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:17237821
Jan 22. Transcription factor TAFII250 promotes Mdm2-dependent turnover of p53.
GO:0005634 nucleus
IDA
PMID:24289924
Phosphorylation of p53 by TAF1 inactivates p53-dependent tra... 		ACCEPT
Summary: p53 is primarily a nuclear transcription factor, with nuclear localization essential for its function.
Reason: Core cellular component - p53's nuclear localization is essential for its transcription factor activity. Contains nuclear localization signals at residues 305-322 and 369-375.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Endoplasmic reticulum.
PMID:14963330
Direct activation of Bax by p53 mediates mitochondrial membrane permeabilization and apoptosis.
PMID:16322561
NIR is a novel INHAT repressor that modulates the transcriptional activity of p53.
PMID:36108750
Phase separation of p53 induced by its unstructured basic region and prevented by oncogenic mutations in tetramerization domain.
PMID:24625977
Ubiquitin-like (UBX)-domain-containing protein, UBXN2A, promotes cell death by interfering with the p53-Mortalin interactions in colon cancer cells.
PMID:26634371
Epub 2015 Dec 4. Structural studies of UBXN2A and mortalin interaction and the putative role of silenced UBXN2A in preventing response to chemotherapy.
PMID:21597459
2011 May 20. E3 ubiquitin ligase Hades negatively regulates the exonuclear function of p53.
PMID:19011621
Arginine methylation regulates the p53 response.
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:24101517
Mitochondrial disulfide relay mediates translocation of p53 and partitions its subcellular activity.
PMID:20810912
Hypoxia downregulates p53 but induces apoptosis and enhances expression of BAD in cultures of human syncytiotrophoblasts.
PMID:19234109
Induction of SOX4 by DNA damage is critical for p53 stabilization and function.
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:17403783
HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53.
PMID:20096447
USP10 regulates p53 localization and stability by deubiquitinating p53.
PMID:18756595
Prognostic significance of BMP and activin membrane-bound inhibitor in colorectal cancer.
PMID:16507995
Myosin VI is a mediator of the p53-dependent cell survival pathway.
PMID:7720704
Direct involvement of p53 in programmed cell death of oligodendrocytes.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-5689950 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-5689973 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005829 cytosol
TAS
Reactome:R-HSA-5690843 		ACCEPT
Summary: p53 localizes to cytosol as part of its nucleo-cytoplasmic shuttling.
Reason: p53 shuttles between nucleus and cytoplasm, with cytosolic localization important for regulation.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus. Cytoplasm
PMID:24101517
Mitochondrial disulfide relay mediates translocation of p53 and partitions its subcellular activity.
PMID:14963330
Direct activation of Bax by p53 mediates mitochondrial membrane permeabilization and apoptosis.
GO:0005829 cytosol
TAS
Reactome:R-HSA-6793685 		ACCEPT
Summary: p53 localizes to cytosol as part of its nucleo-cytoplasmic shuttling.
Reason: p53 shuttles between nucleus and cytoplasm, with cytosolic localization important for regulation.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus. Cytoplasm
PMID:24101517
Mitochondrial disulfide relay mediates translocation of p53 and partitions its subcellular activity.
PMID:14963330
Direct activation of Bax by p53 mediates mitochondrial membrane permeabilization and apoptosis.
GO:0005829 cytosol
TAS
Reactome:R-HSA-8852351 		ACCEPT
Summary: p53 localizes to cytosol as part of its nucleo-cytoplasmic shuttling.
Reason: p53 shuttles between nucleus and cytoplasm, with cytosolic localization important for regulation.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus. Cytoplasm
PMID:24101517
Mitochondrial disulfide relay mediates translocation of p53 and partitions its subcellular activity.
PMID:14963330
Direct activation of Bax by p53 mediates mitochondrial membrane permeabilization and apoptosis.
GO:0005829 cytosol
TAS
Reactome:R-HSA-8852354 		ACCEPT
Summary: p53 localizes to cytosol as part of its nucleo-cytoplasmic shuttling.
Reason: p53 shuttles between nucleus and cytoplasm, with cytosolic localization important for regulation.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus. Cytoplasm
PMID:24101517
Mitochondrial disulfide relay mediates translocation of p53 and partitions its subcellular activity.
PMID:14963330
Direct activation of Bax by p53 mediates mitochondrial membrane permeabilization and apoptosis.
GO:0005515 protein binding
IPI
PMID:10597287
Molecular interactions between telomerase and the tumor supp... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:10597287
Molecular interactions between telomerase and the tumor suppressor protein p53 in vitro.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-3222116 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-3222237 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-5628871 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6805638 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6805730 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6805740 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6805755 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6811508 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6805276 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6805399 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6805103 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6805109 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6805126 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-3215310 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6793685 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6804441 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6804879 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6805059 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-8852337 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-8852351 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-9851077 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6804383 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-3215152 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-3215251 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-3222093 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-3222128 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-3700981 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-3700997 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-4331331 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-4331340 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-5633295 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-5633414 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6791285 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6791302 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6791327 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6791349 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6791387 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6796649 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6797766 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6797993 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6798020 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6798082 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6798129 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6798138 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6798282 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6798304 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6798374 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6799246 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6799409 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6799418 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6799462 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6799761 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6799777 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6799815 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6800042 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6800253 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6800279 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6800836 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6801087 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6801166 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6801209 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6801355 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6801641 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6802165 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6803391 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6803425 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6803719 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6803801 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6803858 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6803914 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6803935 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6804191 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6804242 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6804379 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6804402 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6804425 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6805122 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6805285 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6805620 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6806408 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6806412 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6806413 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6806417 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6806419 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6811479 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-69685 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-9710323 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-9749464 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-9851076 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6798615 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005515 protein binding
IPI
PMID:12730672
Physical and functional interaction between HCV core protein... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:12730672
Physical and functional interaction between HCV core protein and the different p73 isoforms.
GO:0008285 negative regulation of cell population proliferation
IMP
PMID:12730672
Physical and functional interaction between HCV core protein... 		ACCEPT
Summary: p53 negatively regulates cell proliferation as a fundamental tumor suppressor mechanism.
Reason: Core function - p53 inhibits proliferation through cell cycle arrest.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Negatively regulates cell division
PMID:30514107
CELF1/p53 axis: a sustained antiproliferative signal leading to villus atrophy under total parenteral nutrition.
PMID:12730672
Physical and functional interaction between HCV core protein and the different p73 isoforms.
PMID:22783376
Induction of apoptosis by cytoplasmically localized wild-type p53 and the S121F mutant super p53.
GO:1900119 positive regulation of execution phase of apoptosis
IMP
PMID:14985081
Hepatitis C virus core protein interacts with p53-binding pr... 		ACCEPT
Summary: p53 positively regulates intrinsic apoptotic signaling in response to ER stress.
Reason: p53 responds to ER stress and can induce apoptosis under severe ER stress.
Supporting Evidence:
PMID:14985081
Hepatitis C virus core protein interacts with p53-binding protein, 53BP2/Bbp/ASPP2, and inhibits p53-mediated apoptosis.
GO:0043153 entrainment of circadian clock by photoperiod
ISS
GO_REF:0000024 		ACCEPT
Summary: p53 enters the nucleus to function as a transcription factor.
Reason: Core process - nuclear entry is essential for p53's transcription factor activity.
Supporting Evidence:
TP53-deep-research
Nuclear Localization Signals (NLS): Function: Nuclear import and localization
GO:0045892 negative regulation of DNA-templated transcription
IDA
PMID:24051492
p53 regulates Period2 expression and the circadian clock. 		ACCEPT
Summary: p53 negatively regulates transcription of various target genes including anti-apoptotic and cell cycle genes.
Reason: Core function - p53 acts as both a transcriptional activator and repressor.
Supporting Evidence:
TP53-deep-research
Repression: Downregulates anti-apoptotic genes (BCL2), cell cycle progression genes
PMID:16492744
Mdm4 and Mdm2 cooperate to inhibit p53 activity in proliferating and quiescent cells in vivo.
PMID:9271120
Repression of p53-mediated transcription by MDM2: a dual mechanism.
PMID:23629966
Deacetylation of p53 induces autophagy by suppressing Bmf expression.
PMID:24051492
p53 regulates Period2 expression and the circadian clock.
GO:0048512 circadian behavior
ISS
GO_REF:0000024 		KEEP AS NON CORE
Summary: p53 is involved in organ development but is not essential for development.
Reason: Non-core developmental function - p53 knockout mice develop normally.
GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific
IDA
PMID:24652652
DRAGO (KIAA0247), a new DNA damage-responsive, p53-inducible... 		ACCEPT
Summary: p53 is a DNA-binding transcription factor that regulates RNA polymerase II transcription of target genes.
Reason: Core molecular function - p53 is a well-characterized sequence-specific transcription factor for RNA pol II.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:34381247
Epub 2021 Aug 11. The tumor suppressor folliculin inhibits lactate dehydrogenase A and regulates the Warburg effect.
PMID:35618207
Ser392 phosphorylation modulated a switch between p53 and transcriptional condensates.
PMID:36634798
Phosphorylation and specific DNA improved the incorporation ability of p53 into functional condensates.
PMID:38653238
2024 Apr 22. Alanyl-tRNA synthetase, AARS1, is a lactate sensor and lactyltransferase that lactylates p53 and contributes to tumorigenesis.
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:17310983
Feb 19. Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function.
PMID:24652652
2014 Mar 20. DRAGO (KIAA0247), a new DNA damage-responsive, p53-inducible gene that cooperates with p53 as oncosuppressor.
GO:0045944 positive regulation of transcription by RNA polymerase II
IDA
PMID:24652652
DRAGO (KIAA0247), a new DNA damage-responsive, p53-inducible... 		ACCEPT
Summary: p53 is a sequence-specific transcriptional activator that upregulates target genes including p21, PUMA, BAX.
Reason: Fundamental molecular function - p53 activates transcription of numerous target genes through binding to p53 response elements.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:16061649
Ckap2 regulates aneuploidy, cell cycling, and cell death in a p53-dependent manner.
PMID:17599062
Jun 28. CDIP, a novel pro-apoptotic gene, regulates TNFalpha-mediated apoptosis in a p53-dependent manner.
PMID:30089260
p53 Regulates the Expression of LRP1 and Apoptosis through a Stress Intensity-Dependent MicroRNA Feedback Loop.
PMID:7958916
DNA damage triggers a prolonged p53-dependent G1 arrest and long-term induction of Cip1 in normal human fibroblasts.
PMID:11672523
hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase.
PMID:17145718
Brn-3b enhances the pro-apoptotic effects of p53 but not its induction of cell cycle arrest by cooperating in trans-activation of bax expression.
PMID:17146433
Dec 4. RGC32, a novel p53-inducible gene, is located on centrosomes during mitosis and results in G2/M arrest.
PMID:18549481
TATA binding protein associated factor 3 (TAF3) interacts with p53 and inhibits its function.
PMID:24356969
2013 Dec 19. Rbm24, an RNA-binding protein and a target of p53, regulates p21 expression via mRNA stability.
PMID:17310983
Feb 19. Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function.
PMID:24652652
2014 Mar 20. DRAGO (KIAA0247), a new DNA damage-responsive, p53-inducible gene that cooperates with p53 as oncosuppressor.
PMID:20959462
2010 Oct 19. Aurora B interacts with NIR-p53, leading to p53 phosphorylation in its DNA-binding domain and subsequent functional suppression.
GO:0005634 nucleus
IDA
PMID:24101517
Mitochondrial disulfide relay mediates translocation of p53 ... 		ACCEPT
Summary: p53 is primarily a nuclear transcription factor, with nuclear localization essential for its function.
Reason: Core cellular component - p53's nuclear localization is essential for its transcription factor activity. Contains nuclear localization signals at residues 305-322 and 369-375.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Endoplasmic reticulum.
PMID:14963330
Direct activation of Bax by p53 mediates mitochondrial membrane permeabilization and apoptosis.
PMID:16322561
NIR is a novel INHAT repressor that modulates the transcriptional activity of p53.
PMID:36108750
Phase separation of p53 induced by its unstructured basic region and prevented by oncogenic mutations in tetramerization domain.
PMID:24625977
Ubiquitin-like (UBX)-domain-containing protein, UBXN2A, promotes cell death by interfering with the p53-Mortalin interactions in colon cancer cells.
PMID:26634371
Epub 2015 Dec 4. Structural studies of UBXN2A and mortalin interaction and the putative role of silenced UBXN2A in preventing response to chemotherapy.
PMID:21597459
2011 May 20. E3 ubiquitin ligase Hades negatively regulates the exonuclear function of p53.
PMID:19011621
Arginine methylation regulates the p53 response.
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:24101517
Mitochondrial disulfide relay mediates translocation of p53 and partitions its subcellular activity.
PMID:20810912
Hypoxia downregulates p53 but induces apoptosis and enhances expression of BAD in cultures of human syncytiotrophoblasts.
PMID:19234109
Induction of SOX4 by DNA damage is critical for p53 stabilization and function.
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:17403783
HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53.
PMID:20096447
USP10 regulates p53 localization and stability by deubiquitinating p53.
PMID:18756595
Prognostic significance of BMP and activin membrane-bound inhibitor in colorectal cancer.
PMID:16507995
Myosin VI is a mediator of the p53-dependent cell survival pathway.
PMID:7720704
Direct involvement of p53 in programmed cell death of oligodendrocytes.
GO:0005739 mitochondrion
IDA
PMID:24101517
Mitochondrial disulfide relay mediates translocation of p53 ... 		ACCEPT
Summary: p53 translocates to mitochondria to directly promote apoptosis through BAX activation.
Reason: Important localization for non-transcriptional apoptosis - p53 can directly induce MOMP at mitochondria.
Supporting Evidence:
TP53-deep-research
Non-transcriptional: Direct mitochondrial translocation and interaction with BCL-2 family proteins
PMID:25168243
Aug 29. Cofactor Strap regulates oxidative phosphorylation and mitochondrial p53 activity through ATP synthase.
PMID:24101517
Mitochondrial disulfide relay mediates translocation of p53 and partitions its subcellular activity.
PMID:12667443
p53 has a direct apoptogenic role at the mitochondria.
GO:0005829 cytosol
IDA
PMID:24101517
Mitochondrial disulfide relay mediates translocation of p53 ... 		ACCEPT
Summary: p53 localizes to cytosol as part of its nucleo-cytoplasmic shuttling.
Reason: p53 shuttles between nucleus and cytoplasm, with cytosolic localization important for regulation.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus. Cytoplasm
PMID:24101517
Mitochondrial disulfide relay mediates translocation of p53 and partitions its subcellular activity.
PMID:14963330
Direct activation of Bax by p53 mediates mitochondrial membrane permeabilization and apoptosis.
GO:0005634 nucleus
IDA
PMID:20810912
Hypoxia downregulates p53 but induces apoptosis and enhances... 		ACCEPT
Summary: p53 is primarily a nuclear transcription factor, with nuclear localization essential for its function.
Reason: Core cellular component - p53's nuclear localization is essential for its transcription factor activity. Contains nuclear localization signals at residues 305-322 and 369-375.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Endoplasmic reticulum.
PMID:14963330
Direct activation of Bax by p53 mediates mitochondrial membrane permeabilization and apoptosis.
PMID:16322561
NIR is a novel INHAT repressor that modulates the transcriptional activity of p53.
PMID:36108750
Phase separation of p53 induced by its unstructured basic region and prevented by oncogenic mutations in tetramerization domain.
PMID:24625977
Ubiquitin-like (UBX)-domain-containing protein, UBXN2A, promotes cell death by interfering with the p53-Mortalin interactions in colon cancer cells.
PMID:26634371
Epub 2015 Dec 4. Structural studies of UBXN2A and mortalin interaction and the putative role of silenced UBXN2A in preventing response to chemotherapy.
PMID:21597459
2011 May 20. E3 ubiquitin ligase Hades negatively regulates the exonuclear function of p53.
PMID:19011621
Arginine methylation regulates the p53 response.
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:24101517
Mitochondrial disulfide relay mediates translocation of p53 and partitions its subcellular activity.
PMID:20810912
Hypoxia downregulates p53 but induces apoptosis and enhances expression of BAD in cultures of human syncytiotrophoblasts.
PMID:19234109
Induction of SOX4 by DNA damage is critical for p53 stabilization and function.
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:17403783
HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53.
PMID:20096447
USP10 regulates p53 localization and stability by deubiquitinating p53.
PMID:18756595
Prognostic significance of BMP and activin membrane-bound inhibitor in colorectal cancer.
PMID:16507995
Myosin VI is a mediator of the p53-dependent cell survival pathway.
PMID:7720704
Direct involvement of p53 in programmed cell death of oligodendrocytes.
GO:0005737 cytoplasm
IDA
PMID:20810912
Hypoxia downregulates p53 but induces apoptosis and enhances... 		ACCEPT
Summary: p53 localizes to cytoplasm as well as nucleus, with cytoplasmic localization important for regulation.
Reason: Core cellular component - p53 shuttles between nucleus and cytoplasm, with cytoplasmic localization important for MDM2-mediated regulation.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus. Cytoplasm.
PMID:24625977
Ubiquitin-like (UBX)-domain-containing protein, UBXN2A, promotes cell death by interfering with the p53-Mortalin interactions in colon cancer cells.
PMID:26634371
Epub 2015 Dec 4. Structural studies of UBXN2A and mortalin interaction and the putative role of silenced UBXN2A in preventing response to chemotherapy.
PMID:21597459
2011 May 20. E3 ubiquitin ligase Hades negatively regulates the exonuclear function of p53.
PMID:19011621
Arginine methylation regulates the p53 response.
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:23629966
Deacetylation of p53 induces autophagy by suppressing Bmf expression.
PMID:20810912
Hypoxia downregulates p53 but induces apoptosis and enhances expression of BAD in cultures of human syncytiotrophoblasts.
PMID:16131611
p53 isoforms can regulate p53 transcriptional activity.
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:20096447
USP10 regulates p53 localization and stability by deubiquitinating p53.
PMID:7720704
Direct involvement of p53 in programmed cell death of oligodendrocytes.
GO:0006974 DNA damage response
IDA
PMID:17938203
Protein kinase C delta induces transcription of the TP53 tum... 		ACCEPT
Summary: p53 is central to the DNA damage response, being activated by and coordinating responses to DNA damage.
Reason: Core function - p53 is the 'guardian of the genome' that coordinates DNA damage responses.
Supporting Evidence:
TP53-deep-research
p53 serves as a central node in the DNA damage response network
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:30089260
p53 Regulates the Expression of LRP1 and Apoptosis through a Stress Intensity-Dependent MicroRNA Feedback Loop.
PMID:24356969
2013 Dec 19. Rbm24, an RNA-binding protein and a target of p53, regulates p21 expression via mRNA stability.
PMID:17938203
Oct 15. Protein kinase C delta induces transcription of the TP53 tumor suppressor gene by controlling death-promoting factor Btf in the apoptotic response to DNA damage.
PMID:15710329
Human MUC1 oncoprotein regulates p53-responsive gene transcription in the genotoxic stress response.
GO:0046677 response to antibiotic
IEP
PMID:20810912
Hypoxia downregulates p53 but induces apoptosis and enhances... 		ACCEPT
Summary: p53 responds to antibiotic stress that causes cellular stress or DNA damage.
Reason: p53 responds to various xenobiotic stresses including antibiotics.
Supporting Evidence:
PMID:20810912
Hypoxia downregulates p53 but induces apoptosis and enhances expression of BAD in cultures of human syncytiotrophoblasts.
GO:0071456 cellular response to hypoxia
IEP
PMID:20810912
Hypoxia downregulates p53 but induces apoptosis and enhances... 		ACCEPT
Summary: p53 mediates cellular responses to hypoxia, including cell cycle arrest and apoptosis induction.
Reason: Core stress response function - p53 responds to hypoxic stress and coordinates appropriate cellular responses.
Supporting Evidence:
GO:1990144
intrinsic apoptotic signaling pathway in response to hypoxia
PMID:20810912
Hypoxia downregulates p53 but induces apoptosis and enhances expression of BAD in cultures of human syncytiotrophoblasts.
GO:0090200 positive regulation of release of cytochrome c from mitochondria
IDA
PMID:14963330
Direct activation of Bax by p53 mediates mitochondrial membr... 		ACCEPT
Summary: p53 positively regulates mitochondrial DNA replication through its DNA repair functions.
Reason: p53 maintains mitochondrial genome stability through various mechanisms.
Supporting Evidence:
PMID:14963330
Direct activation of Bax by p53 mediates mitochondrial membrane permeabilization and apoptosis.
GO:2001244 positive regulation of intrinsic apoptotic signaling pathway
IMP
PMID:14963330
Direct activation of Bax by p53 mediates mitochondrial membr... 		ACCEPT
Summary: p53 negatively regulates intrinsic apoptotic signaling in certain contexts.
Reason: p53 can have context-dependent anti-apoptotic effects through p21-mediated survival.
Supporting Evidence:
PMID:14963330
Direct activation of Bax by p53 mediates mitochondrial membrane permeabilization and apoptosis.
GO:0072332 intrinsic apoptotic signaling pathway by p53 class mediator
IMP
PMID:16322561
NIR is a novel INHAT repressor that modulates the transcript... 		ACCEPT
Summary: p53 mediates intrinsic apoptotic signaling through BAX, PUMA, NOXA activation.
Reason: Core apoptotic function - p53 is the master regulator of intrinsic apoptosis.
Supporting Evidence:
TP53-deep-research
Transcriptional: Activation of pro-apoptotic genes (PUMA, BAX, NOXA)
PMID:17310983
Feb 19. Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function.
PMID:16322561
NIR is a novel INHAT repressor that modulates the transcriptional activity of p53.
PMID:12172011
Chk2 regulates irradiation-induced, p53-mediated apoptosis in Drosophila.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-3222259 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-3239014 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005515 protein binding
IPI
PMID:17719541
Hzf Determines cell survival upon genotoxic stress by modula... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:17719541
Hzf Determines cell survival upon genotoxic stress by modulating p53 transactivation.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-264435 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-3221982 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-3222006 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-3222020 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-3786258 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-4395236 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-4647593 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-5628899 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-5629187 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-5632759 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-5632887 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-5632914 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-5632939 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-5633460 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-5693609 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6789960 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6791409 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6799332 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6799431 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6804188 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6804762 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6804996 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6804998 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6805035 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6805470 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-6805479 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-8853911 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-HSA-8952128 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
TAS
Reactome:R-NUL-992753 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0097252 oligodendrocyte apoptotic process
IDA
PMID:7720704
Direct involvement of p53 in programmed cell death of oligod... 		ACCEPT
Summary: p53 undergoes various post-translational modifications in response to stress.
Reason: Core regulatory mechanism - p53 is extensively modified at over 60 residues.
Supporting Evidence:
TP53-deep-research
p53 can be modified at over 60 of its 393 residues
PMID:7720704
Direct involvement of p53 in programmed cell death of oligodendrocytes.
GO:0005515 protein binding
IPI
PMID:22945289
ZNF385B is characteristically expressed in germinal center B... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:22945289
Oct 16. ZNF385B is characteristically expressed in germinal center B cells and involved in B-cell apoptosis.
GO:0045893 positive regulation of DNA-templated transcription
IDA
PMID:20378837
Glutaminase 2, a novel p53 target gene regulating energy met... 		ACCEPT
Summary: p53 positively regulates DNA-templated transcription of target genes.
Reason: Core transcriptional function - p53 activates transcription of numerous target genes.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:20378837
Glutaminase 2, a novel p53 target gene regulating energy metabolism and antioxidant function.
PMID:16322561
NIR is a novel INHAT repressor that modulates the transcriptional activity of p53.
PMID:17403783
HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53.
PMID:20096447
USP10 regulates p53 localization and stability by deubiquitinating p53.
GO:0008285 negative regulation of cell population proliferation
IDA
PMID:22783376
Induction of apoptosis by cytoplasmically localized wild-typ... 		ACCEPT
Summary: p53 negatively regulates cell proliferation as a fundamental tumor suppressor mechanism.
Reason: Core function - p53 inhibits proliferation through cell cycle arrest.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Negatively regulates cell division
PMID:30514107
CELF1/p53 axis: a sustained antiproliferative signal leading to villus atrophy under total parenteral nutrition.
PMID:12730672
Physical and functional interaction between HCV core protein and the different p73 isoforms.
PMID:22783376
Induction of apoptosis by cytoplasmically localized wild-type p53 and the S121F mutant super p53.
GO:0005515 protein binding
IPI
PMID:23431171
MOZ increases p53 acetylation and premature senescence throu... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:23431171
MOZ increases p53 acetylation and premature senescence through its complex formation with PML.
GO:0016605 PML body
IDA
PMID:22869143
BMK1 is involved in the regulation of p53 through disrupting... 		ACCEPT
Summary: p53 localizes to PML nuclear bodies, which is important for its post-translational modification and activation.
Reason: Important regulatory localization - p53 accumulates in PML bodies during stress responses where it undergoes acetylation.
Supporting Evidence:
TP53-deep-research
p53 localizes to nuclear bodies like PML bodies, especially during stress responses
PMID:22869143
BMK1 is involved in the regulation of p53 through disrupting the PML-MDM2 interaction.
PMID:12006491
Human SIR2 deacetylates p53 and antagonizes PML/p53-induced cellular senescence.
GO:0043066 negative regulation of apoptotic process
IMP
PMID:20124405
G-protein-coupled receptor kinase 5 phosphorylates p53 and i... 		ACCEPT
Summary: p53 can negatively regulate apoptosis in certain contexts through p21-mediated survival.
Reason: Context-dependent function - p53 can promote survival under mild stress conditions.
Supporting Evidence:
PMID:20124405
2010 Feb 2. G-protein-coupled receptor kinase 5 phosphorylates p53 and inhibits DNA damage-induced apoptosis.
GO:0016032 viral process
IMP
PMID:22574148
p53 Activation following Rift Valley fever virus infection c... 		KEEP AS NON CORE
Summary: p53 is involved in viral processes through its antiviral functions.
Reason: Non-core function - p53 has antiviral activity but this is not its primary role.
Supporting Evidence:
PMID:22574148
p53 Activation following Rift Valley fever virus infection contributes to cell death and viral production.
GO:0000785 chromatin
IDA
PMID:22521434
Hepatocyte growth factor (HGF) and 1,25-dihydroxyvitamin D t... 		ACCEPT
Summary: p53 associates with chromatin at target gene promoters to regulate transcription.
Reason: Core molecular function - p53 binds to chromatin at p53 response elements to regulate target gene expression.
Supporting Evidence:
PDB:3TS8
Crystal structure of a multidomain human p53 tetramer bound to the natural CDKN1A(p21) p53-response element
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:30089260
p53 Regulates the Expression of LRP1 and Apoptosis through a Stress Intensity-Dependent MicroRNA Feedback Loop.
PMID:23629966
Deacetylation of p53 induces autophagy by suppressing Bmf expression.
PMID:22521434
Hepatocyte growth factor (HGF) and 1,25-dihydroxyvitamin D together stimulate human bone marrow-derived stem cells toward the osteogenic phenotype by HGF-induced up-regulation of VDR.
PMID:15710329
Human MUC1 oncoprotein regulates p53-responsive gene transcription in the genotoxic stress response.
PMID:17805299
p53 is regulated by the lysine demethylase LSD1.
GO:0030971 receptor tyrosine kinase binding
IPI
PMID:23027130
TrkC signaling is activated in adenoid cystic carcinoma and ... 		ACCEPT
Summary: p53 interacts with receptor tyrosine kinases as part of signal transduction.
Reason: p53 integrates signals from growth factor signaling pathways.
Supporting Evidence:
PMID:23027130
TrkC signaling is activated in adenoid cystic carcinoma and requires NT-3 to stimulate invasive behavior.
GO:0005515 protein binding
IPI
PMID:19483087
Identification and characterization of two novel isoforms of... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:19483087
Epub 2009 May 29. Identification and characterization of two novel isoforms of Pirh2 ubiquitin ligase that negatively regulate p53 independent of RING finger domains.
GO:0005515 protein binding
IPI
PMID:20096447
USP10 regulates p53 localization and stability by deubiquiti... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:20096447
USP10 regulates p53 localization and stability by deubiquitinating p53.
GO:0005829 cytosol
IDA
PMID:14963330
Direct activation of Bax by p53 mediates mitochondrial membr... 		ACCEPT
Summary: p53 localizes to cytosol as part of its nucleo-cytoplasmic shuttling.
Reason: p53 shuttles between nucleus and cytoplasm, with cytosolic localization important for regulation.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus. Cytoplasm
PMID:24101517
Mitochondrial disulfide relay mediates translocation of p53 and partitions its subcellular activity.
PMID:14963330
Direct activation of Bax by p53 mediates mitochondrial membrane permeabilization and apoptosis.
GO:0000785 chromatin
IDA
PMID:15710329
Human MUC1 oncoprotein regulates p53-responsive gene transcr... 		ACCEPT
Summary: p53 associates with chromatin at target gene promoters to regulate transcription.
Reason: Core molecular function - p53 binds to chromatin at p53 response elements to regulate target gene expression.
Supporting Evidence:
PDB:3TS8
Crystal structure of a multidomain human p53 tetramer bound to the natural CDKN1A(p21) p53-response element
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:30089260
p53 Regulates the Expression of LRP1 and Apoptosis through a Stress Intensity-Dependent MicroRNA Feedback Loop.
PMID:23629966
Deacetylation of p53 induces autophagy by suppressing Bmf expression.
PMID:22521434
Hepatocyte growth factor (HGF) and 1,25-dihydroxyvitamin D together stimulate human bone marrow-derived stem cells toward the osteogenic phenotype by HGF-induced up-regulation of VDR.
PMID:15710329
Human MUC1 oncoprotein regulates p53-responsive gene transcription in the genotoxic stress response.
PMID:17805299
p53 is regulated by the lysine demethylase LSD1.
GO:0005515 protein binding
IPI
PMID:15710329
Human MUC1 oncoprotein regulates p53-responsive gene transcr... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:15710329
Human MUC1 oncoprotein regulates p53-responsive gene transcription in the genotoxic stress response.
GO:0006974 DNA damage response
IDA
PMID:15710329
Human MUC1 oncoprotein regulates p53-responsive gene transcr... 		ACCEPT
Summary: p53 is central to the DNA damage response, being activated by and coordinating responses to DNA damage.
Reason: Core function - p53 is the 'guardian of the genome' that coordinates DNA damage responses.
Supporting Evidence:
TP53-deep-research
p53 serves as a central node in the DNA damage response network
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:30089260
p53 Regulates the Expression of LRP1 and Apoptosis through a Stress Intensity-Dependent MicroRNA Feedback Loop.
PMID:24356969
2013 Dec 19. Rbm24, an RNA-binding protein and a target of p53, regulates p21 expression via mRNA stability.
PMID:17938203
Oct 15. Protein kinase C delta induces transcription of the TP53 tumor suppressor gene by controlling death-promoting factor Btf in the apoptotic response to DNA damage.
PMID:15710329
Human MUC1 oncoprotein regulates p53-responsive gene transcription in the genotoxic stress response.
GO:0048147 negative regulation of fibroblast proliferation
IMP
PMID:10962037
Overexpression of MYC causes p53-dependent G2 arrest of norm... 		KEEP AS NON CORE
Summary: p53 negatively regulates fibroblast proliferation as part of its anti-proliferative function.
Reason: Cell type-specific manifestation of anti-proliferative function.
Supporting Evidence:
PMID:10962037
Overexpression of MYC causes p53-dependent G2 arrest of normal fibroblasts.
GO:0005515 protein binding
IPI
PMID:20123734
NIR, an inhibitor of histone acetyltransferases, regulates t... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:20123734
Jan 31. NIR, an inhibitor of histone acetyltransferases, regulates transcription factor TAp63 and is controlled by the cell cycle.
GO:0005515 protein binding
IPI
PMID:20959462
Aurora B interacts with NIR-p53, leading to p53 phosphorylat... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:20959462
2010 Oct 19. Aurora B interacts with NIR-p53, leading to p53 phosphorylation in its DNA-binding domain and subsequent functional suppression.
GO:0043065 positive regulation of apoptotic process
IDA
PMID:20959462
Aurora B interacts with NIR-p53, leading to p53 phosphorylat... 		ACCEPT
Summary: p53 positively regulates apoptosis as a major tumor suppressor mechanism.
Reason: Core function - p53 induces apoptosis through multiple pathways.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression
PMID:12667443
p53 has a direct apoptogenic role at the mitochondria.
PMID:15565177
A novel mitochondrial protein DIP mediates E2F1-induced apoptosis independently of p53.
PMID:20959462
2010 Oct 19. Aurora B interacts with NIR-p53, leading to p53 phosphorylation in its DNA-binding domain and subsequent functional suppression.
GO:0045944 positive regulation of transcription by RNA polymerase II
IDA
PMID:20959462
Aurora B interacts with NIR-p53, leading to p53 phosphorylat... 		ACCEPT
Summary: p53 is a sequence-specific transcriptional activator that upregulates target genes including p21, PUMA, BAX.
Reason: Fundamental molecular function - p53 activates transcription of numerous target genes through binding to p53 response elements.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:16061649
Ckap2 regulates aneuploidy, cell cycling, and cell death in a p53-dependent manner.
PMID:17599062
Jun 28. CDIP, a novel pro-apoptotic gene, regulates TNFalpha-mediated apoptosis in a p53-dependent manner.
PMID:30089260
p53 Regulates the Expression of LRP1 and Apoptosis through a Stress Intensity-Dependent MicroRNA Feedback Loop.
PMID:7958916
DNA damage triggers a prolonged p53-dependent G1 arrest and long-term induction of Cip1 in normal human fibroblasts.
PMID:11672523
hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase.
PMID:17145718
Brn-3b enhances the pro-apoptotic effects of p53 but not its induction of cell cycle arrest by cooperating in trans-activation of bax expression.
PMID:17146433
Dec 4. RGC32, a novel p53-inducible gene, is located on centrosomes during mitosis and results in G2/M arrest.
PMID:18549481
TATA binding protein associated factor 3 (TAF3) interacts with p53 and inhibits its function.
PMID:24356969
2013 Dec 19. Rbm24, an RNA-binding protein and a target of p53, regulates p21 expression via mRNA stability.
PMID:17310983
Feb 19. Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function.
PMID:24652652
2014 Mar 20. DRAGO (KIAA0247), a new DNA damage-responsive, p53-inducible gene that cooperates with p53 as oncosuppressor.
PMID:20959462
2010 Oct 19. Aurora B interacts with NIR-p53, leading to p53 phosphorylation in its DNA-binding domain and subsequent functional suppression.
GO:0003682 chromatin binding
IDA
PMID:16322561
NIR is a novel INHAT repressor that modulates the transcript... 		ACCEPT
Summary: p53 binds chromatin at p53 response elements in target gene promoters.
Reason: Core molecular function - chromatin binding is essential for p53's transcriptional regulation.
Supporting Evidence:
PDB:3TS8
Crystal structure of p53 tetramer bound to DNA
PMID:26334721
2015 Sep 3. Jmjd5 functions as a regulator of p53 signaling during mouse embryogenesis.
PMID:16322561
NIR is a novel INHAT repressor that modulates the transcriptional activity of p53.
PMID:17599062
Jun 28. CDIP, a novel pro-apoptotic gene, regulates TNFalpha-mediated apoptosis in a p53-dependent manner.
GO:0045893 positive regulation of DNA-templated transcription
IDA
PMID:16322561
NIR is a novel INHAT repressor that modulates the transcript... 		ACCEPT
Summary: p53 positively regulates DNA-templated transcription of target genes.
Reason: Core transcriptional function - p53 activates transcription of numerous target genes.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:20378837
Glutaminase 2, a novel p53 target gene regulating energy metabolism and antioxidant function.
PMID:16322561
NIR is a novel INHAT repressor that modulates the transcriptional activity of p53.
PMID:17403783
HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53.
PMID:20096447
USP10 regulates p53 localization and stability by deubiquitinating p53.
GO:0005515 protein binding
IPI
PMID:20124405
G-protein-coupled receptor kinase 5 phosphorylates p53 and i... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:20124405
2010 Feb 2. G-protein-coupled receptor kinase 5 phosphorylates p53 and inhibits DNA damage-induced apoptosis.
GO:0042149 cellular response to glucose starvation
IDA
PMID:21471221
Novel nucleolar pathway connecting intracellular energy stat... 		ACCEPT
Summary: p53 responds to glucose starvation as part of metabolic stress response.
Reason: p53 responds to metabolic stress including glucose deprivation.
Supporting Evidence:
PMID:21471221
2011 Apr 6. Novel nucleolar pathway connecting intracellular energy status with p53 activation.
GO:0005634 nucleus
IDA
PMID:19234109
Induction of SOX4 by DNA damage is critical for p53 stabiliz... 		ACCEPT
Summary: p53 is primarily a nuclear transcription factor, with nuclear localization essential for its function.
Reason: Core cellular component - p53's nuclear localization is essential for its transcription factor activity. Contains nuclear localization signals at residues 305-322 and 369-375.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Endoplasmic reticulum.
PMID:14963330
Direct activation of Bax by p53 mediates mitochondrial membrane permeabilization and apoptosis.
PMID:16322561
NIR is a novel INHAT repressor that modulates the transcriptional activity of p53.
PMID:36108750
Phase separation of p53 induced by its unstructured basic region and prevented by oncogenic mutations in tetramerization domain.
PMID:24625977
Ubiquitin-like (UBX)-domain-containing protein, UBXN2A, promotes cell death by interfering with the p53-Mortalin interactions in colon cancer cells.
PMID:26634371
Epub 2015 Dec 4. Structural studies of UBXN2A and mortalin interaction and the putative role of silenced UBXN2A in preventing response to chemotherapy.
PMID:21597459
2011 May 20. E3 ubiquitin ligase Hades negatively regulates the exonuclear function of p53.
PMID:19011621
Arginine methylation regulates the p53 response.
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:24101517
Mitochondrial disulfide relay mediates translocation of p53 and partitions its subcellular activity.
PMID:20810912
Hypoxia downregulates p53 but induces apoptosis and enhances expression of BAD in cultures of human syncytiotrophoblasts.
PMID:19234109
Induction of SOX4 by DNA damage is critical for p53 stabilization and function.
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:17403783
HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53.
PMID:20096447
USP10 regulates p53 localization and stability by deubiquitinating p53.
PMID:18756595
Prognostic significance of BMP and activin membrane-bound inhibitor in colorectal cancer.
PMID:16507995
Myosin VI is a mediator of the p53-dependent cell survival pathway.
PMID:7720704
Direct involvement of p53 in programmed cell death of oligodendrocytes.
GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding
IPI
PMID:18549481
TATA binding protein associated factor 3 (TAF3) interacts wi... 		ACCEPT
Summary: p53 binds RNA through its C-terminal domain.
Reason: p53 has RNA-binding activity that contributes to regulation of gene expression.
Supporting Evidence:
PMID:18549481
TATA binding protein associated factor 3 (TAF3) interacts with p53 and inhibits its function.
GO:0072332 intrinsic apoptotic signaling pathway by p53 class mediator
IMP
PMID:12172011
Chk2 regulates irradiation-induced, p53-mediated apoptosis i... 		ACCEPT
Summary: p53 mediates intrinsic apoptotic signaling through BAX, PUMA, NOXA activation.
Reason: Core apoptotic function - p53 is the master regulator of intrinsic apoptosis.
Supporting Evidence:
TP53-deep-research
Transcriptional: Activation of pro-apoptotic genes (PUMA, BAX, NOXA)
PMID:17310983
Feb 19. Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function.
PMID:16322561
NIR is a novel INHAT repressor that modulates the transcriptional activity of p53.
PMID:12172011
Chk2 regulates irradiation-induced, p53-mediated apoptosis in Drosophila.
GO:0000785 chromatin
IDA
PMID:17805299
p53 is regulated by the lysine demethylase LSD1. 		ACCEPT
Summary: p53 associates with chromatin at target gene promoters to regulate transcription.
Reason: Core molecular function - p53 binds to chromatin at p53 response elements to regulate target gene expression.
Supporting Evidence:
PDB:3TS8
Crystal structure of a multidomain human p53 tetramer bound to the natural CDKN1A(p21) p53-response element
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:30089260
p53 Regulates the Expression of LRP1 and Apoptosis through a Stress Intensity-Dependent MicroRNA Feedback Loop.
PMID:23629966
Deacetylation of p53 induces autophagy by suppressing Bmf expression.
PMID:22521434
Hepatocyte growth factor (HGF) and 1,25-dihydroxyvitamin D together stimulate human bone marrow-derived stem cells toward the osteogenic phenotype by HGF-induced up-regulation of VDR.
PMID:15710329
Human MUC1 oncoprotein regulates p53-responsive gene transcription in the genotoxic stress response.
PMID:17805299
p53 is regulated by the lysine demethylase LSD1.
GO:0019899 enzyme binding
IPI
PMID:17805299
p53 is regulated by the lysine demethylase LSD1. 		REMOVE
Summary: Generic 'enzyme binding' is uninformative for p53 function.
Reason: Too generic - p53 binds many enzymes but this term provides no functional insight.
Supporting Evidence:
PMID:17805299
p53 is regulated by the lysine demethylase LSD1.
GO:0071466 cellular response to xenobiotic stimulus
IEP
PMID:20810912
Hypoxia downregulates p53 but induces apoptosis and enhances... 		ACCEPT
Summary: p53 mediates cellular response to xenobiotic stress.
Reason: p53 responds to various xenobiotic stresses that cause DNA damage or cellular stress.
Supporting Evidence:
PMID:20810912
Hypoxia downregulates p53 but induces apoptosis and enhances expression of BAD in cultures of human syncytiotrophoblasts.
GO:2000379 positive regulation of reactive oxygen species metabolic process
IMP
PMID:20160708
Feedback between p21 and reactive oxygen production is neces... 		ACCEPT
Summary: p53 positively regulates intrinsic apoptotic signaling in response to DNA damage.
Reason: Core function - DNA damage-induced apoptosis is a primary p53 tumor suppressor mechanism.
Supporting Evidence:
GO:0042771
intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
PMID:20160708
Feedback between p21 and reactive oxygen production is necessary for cell senescence.
GO:0005737 cytoplasm
IDA
PMID:16131611
p53 isoforms can regulate p53 transcriptional activity. 		ACCEPT
Summary: p53 localizes to cytoplasm as well as nucleus, with cytoplasmic localization important for regulation.
Reason: Core cellular component - p53 shuttles between nucleus and cytoplasm, with cytoplasmic localization important for MDM2-mediated regulation.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus. Cytoplasm.
PMID:24625977
Ubiquitin-like (UBX)-domain-containing protein, UBXN2A, promotes cell death by interfering with the p53-Mortalin interactions in colon cancer cells.
PMID:26634371
Epub 2015 Dec 4. Structural studies of UBXN2A and mortalin interaction and the putative role of silenced UBXN2A in preventing response to chemotherapy.
PMID:21597459
2011 May 20. E3 ubiquitin ligase Hades negatively regulates the exonuclear function of p53.
PMID:19011621
Arginine methylation regulates the p53 response.
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:23629966
Deacetylation of p53 induces autophagy by suppressing Bmf expression.
PMID:20810912
Hypoxia downregulates p53 but induces apoptosis and enhances expression of BAD in cultures of human syncytiotrophoblasts.
PMID:16131611
p53 isoforms can regulate p53 transcriptional activity.
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:20096447
USP10 regulates p53 localization and stability by deubiquitinating p53.
PMID:7720704
Direct involvement of p53 in programmed cell death of oligodendrocytes.
GO:0008285 negative regulation of cell population proliferation
ISS
GO_REF:0000024 		ACCEPT
Summary: p53 negatively regulates cell proliferation as a fundamental tumor suppressor mechanism.
Reason: Core function - p53 inhibits proliferation through cell cycle arrest.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Negatively regulates cell division
PMID:30514107
CELF1/p53 axis: a sustained antiproliferative signal leading to villus atrophy under total parenteral nutrition.
PMID:12730672
Physical and functional interaction between HCV core protein and the different p73 isoforms.
PMID:22783376
Induction of apoptosis by cytoplasmically localized wild-type p53 and the S121F mutant super p53.
GO:0008340 determination of adult lifespan
ISS
GO_REF:0000024 		KEEP AS NON CORE
Summary: p53 may influence lifespan through its regulation of senescence and apoptosis.
Reason: Non-core function - while p53 influences aging, this is secondary to its tumor suppressor function.
GO:0070245 positive regulation of thymocyte apoptotic process
ISS
GO_REF:0000024 		ACCEPT
Summary: p53 responds to positive regulation by stress-induced post-translational modifications.
Reason: p53 is regulated by extensive post-translational modifications that enhance its activity.
Supporting Evidence:
TP53-deep-research
p53 can be modified at over 60 of its 393 residues
GO:0005515 protein binding
IPI
PMID:17707234
Modulation of p53 function by SET8-mediated methylation at l... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:17707234
Modulation of p53 function by SET8-mediated methylation at lysine 382.
GO:0005515 protein binding
IPI
PMID:20118233
G9a and Glp methylate lysine 373 in the tumor suppressor p53... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:20118233
2010 Jan 29. G9a and Glp methylate lysine 373 in the tumor suppressor p53.
GO:0090399 replicative senescence
IMP
PMID:19951988
WNT16B is a new marker of cellular senescence that regulates... 		ACCEPT
Summary: p53 is stabilized and activated under stress conditions.
Reason: Core mechanism - p53 protein stabilization is essential for its tumor suppressor function.
Supporting Evidence:
TP53-deep-research
p53 is activated through post-translational modifications which stabilize the protein
PMID:19951988
WNT16B is a new marker of cellular senescence that regulates p53 activity and the phosphoinositide 3-kinase/AKT pathway.
GO:0090403 oxidative stress-induced premature senescence
IMP
PMID:19951988
WNT16B is a new marker of cellular senescence that regulates... 		ACCEPT
Summary: p53 induces oxidative stress during apoptosis induction.
Reason: p53 regulates cellular redox and can promote ROS production in apoptosis.
Supporting Evidence:
PMID:19951988
WNT16B is a new marker of cellular senescence that regulates p53 activity and the phosphoinositide 3-kinase/AKT pathway.
GO:0005634 nucleus
IDA
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localiz... 		ACCEPT
Summary: p53 is primarily a nuclear transcription factor, with nuclear localization essential for its function.
Reason: Core cellular component - p53's nuclear localization is essential for its transcription factor activity. Contains nuclear localization signals at residues 305-322 and 369-375.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Endoplasmic reticulum.
PMID:14963330
Direct activation of Bax by p53 mediates mitochondrial membrane permeabilization and apoptosis.
PMID:16322561
NIR is a novel INHAT repressor that modulates the transcriptional activity of p53.
PMID:36108750
Phase separation of p53 induced by its unstructured basic region and prevented by oncogenic mutations in tetramerization domain.
PMID:24625977
Ubiquitin-like (UBX)-domain-containing protein, UBXN2A, promotes cell death by interfering with the p53-Mortalin interactions in colon cancer cells.
PMID:26634371
Epub 2015 Dec 4. Structural studies of UBXN2A and mortalin interaction and the putative role of silenced UBXN2A in preventing response to chemotherapy.
PMID:21597459
2011 May 20. E3 ubiquitin ligase Hades negatively regulates the exonuclear function of p53.
PMID:19011621
Arginine methylation regulates the p53 response.
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:24101517
Mitochondrial disulfide relay mediates translocation of p53 and partitions its subcellular activity.
PMID:20810912
Hypoxia downregulates p53 but induces apoptosis and enhances expression of BAD in cultures of human syncytiotrophoblasts.
PMID:19234109
Induction of SOX4 by DNA damage is critical for p53 stabilization and function.
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:17403783
HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53.
PMID:20096447
USP10 regulates p53 localization and stability by deubiquitinating p53.
PMID:18756595
Prognostic significance of BMP and activin membrane-bound inhibitor in colorectal cancer.
PMID:16507995
Myosin VI is a mediator of the p53-dependent cell survival pathway.
PMID:7720704
Direct involvement of p53 in programmed cell death of oligodendrocytes.
GO:0005737 cytoplasm
IDA
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localiz... 		ACCEPT
Summary: p53 localizes to cytoplasm as well as nucleus, with cytoplasmic localization important for regulation.
Reason: Core cellular component - p53 shuttles between nucleus and cytoplasm, with cytoplasmic localization important for MDM2-mediated regulation.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus. Cytoplasm.
PMID:24625977
Ubiquitin-like (UBX)-domain-containing protein, UBXN2A, promotes cell death by interfering with the p53-Mortalin interactions in colon cancer cells.
PMID:26634371
Epub 2015 Dec 4. Structural studies of UBXN2A and mortalin interaction and the putative role of silenced UBXN2A in preventing response to chemotherapy.
PMID:21597459
2011 May 20. E3 ubiquitin ligase Hades negatively regulates the exonuclear function of p53.
PMID:19011621
Arginine methylation regulates the p53 response.
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:23629966
Deacetylation of p53 induces autophagy by suppressing Bmf expression.
PMID:20810912
Hypoxia downregulates p53 but induces apoptosis and enhances expression of BAD in cultures of human syncytiotrophoblasts.
PMID:16131611
p53 isoforms can regulate p53 transcriptional activity.
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:20096447
USP10 regulates p53 localization and stability by deubiquitinating p53.
PMID:7720704
Direct involvement of p53 in programmed cell death of oligodendrocytes.
GO:0005634 nucleus
IDA
PMID:17403783
HLA-B-associated transcript 3 (Bat3)/Scythe is essential for... 		ACCEPT
Summary: p53 is primarily a nuclear transcription factor, with nuclear localization essential for its function.
Reason: Core cellular component - p53's nuclear localization is essential for its transcription factor activity. Contains nuclear localization signals at residues 305-322 and 369-375.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Endoplasmic reticulum.
PMID:14963330
Direct activation of Bax by p53 mediates mitochondrial membrane permeabilization and apoptosis.
PMID:16322561
NIR is a novel INHAT repressor that modulates the transcriptional activity of p53.
PMID:36108750
Phase separation of p53 induced by its unstructured basic region and prevented by oncogenic mutations in tetramerization domain.
PMID:24625977
Ubiquitin-like (UBX)-domain-containing protein, UBXN2A, promotes cell death by interfering with the p53-Mortalin interactions in colon cancer cells.
PMID:26634371
Epub 2015 Dec 4. Structural studies of UBXN2A and mortalin interaction and the putative role of silenced UBXN2A in preventing response to chemotherapy.
PMID:21597459
2011 May 20. E3 ubiquitin ligase Hades negatively regulates the exonuclear function of p53.
PMID:19011621
Arginine methylation regulates the p53 response.
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:24101517
Mitochondrial disulfide relay mediates translocation of p53 and partitions its subcellular activity.
PMID:20810912
Hypoxia downregulates p53 but induces apoptosis and enhances expression of BAD in cultures of human syncytiotrophoblasts.
PMID:19234109
Induction of SOX4 by DNA damage is critical for p53 stabilization and function.
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:17403783
HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53.
PMID:20096447
USP10 regulates p53 localization and stability by deubiquitinating p53.
PMID:18756595
Prognostic significance of BMP and activin membrane-bound inhibitor in colorectal cancer.
PMID:16507995
Myosin VI is a mediator of the p53-dependent cell survival pathway.
PMID:7720704
Direct involvement of p53 in programmed cell death of oligodendrocytes.
GO:0042771 intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
IDA
PMID:17403783
HLA-B-associated transcript 3 (Bat3)/Scythe is essential for... 		ACCEPT
Summary: p53 is THE p53 class mediator that induces intrinsic apoptosis in response to DNA damage through BAX, PUMA, NOXA activation.
Reason: Absolutely core function - this term specifically describes p53's role as the central mediator of DNA damage-induced apoptosis.
Supporting Evidence:
TP53-deep-research
Transcriptional: Activation of pro-apoptotic genes (PUMA, BAX, NOXA, FAS)
PMID:14654789
A member of the Pyrin family, IFI16, is a novel BRCA1-associated protein involved in the p53-mediated apoptosis pathway.
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:16213212
Regulation of p53 translation and induction after DNA damage by ribosomal protein L26 and nucleolin.
PMID:17403783
HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53.
GO:0045893 positive regulation of DNA-templated transcription
IDA
PMID:17403783
HLA-B-associated transcript 3 (Bat3)/Scythe is essential for... 		ACCEPT
Summary: p53 positively regulates DNA-templated transcription of target genes.
Reason: Core transcriptional function - p53 activates transcription of numerous target genes.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:20378837
Glutaminase 2, a novel p53 target gene regulating energy metabolism and antioxidant function.
PMID:16322561
NIR is a novel INHAT repressor that modulates the transcriptional activity of p53.
PMID:17403783
HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53.
PMID:20096447
USP10 regulates p53 localization and stability by deubiquitinating p53.
GO:0071479 cellular response to ionizing radiation
IMP
PMID:20160708
Feedback between p21 and reactive oxygen production is neces... 		ACCEPT
Summary: p53 responds to UV-A radiation-induced DNA damage.
Reason: Core DNA damage response function - UV radiation is a classic p53 activator.
Supporting Evidence:
GO:0009411
response to UV
PMID:20160708
Feedback between p21 and reactive oxygen production is necessary for cell senescence.
GO:0000122 negative regulation of transcription by RNA polymerase II
ISS
PMID:19749791
Repression of SHP-1 expression by p53 leads to trkA tyrosine... 		ACCEPT
Summary: p53 functions as a transcriptional repressor for genes including BCL2 and cell cycle genes.
Reason: Core function - transcriptional repression is part of p53's regulatory activity.
Supporting Evidence:
TP53-deep-research
Repression: Downregulates anti-apoptotic genes (BCL2), cell cycle progression genes
PMID:10329733
p53 suppresses the activation of the Bcl-2 promoter by the Brn-3a POU family transcription factor.
PMID:19749791
Sep 14. Repression of SHP-1 expression by p53 leads to trkA tyrosine phosphorylation and suppression of breast cancer cell proliferation.
GO:0002020 protease binding
IPI
PMID:11923872
Deubiquitination of p53 by HAUSP is an important pathway for... 		ACCEPT
Summary: p53 binds protease inhibitors as part of regulatory interactions.
Reason: Regulatory protein-protein interaction.
Supporting Evidence:
PMID:11923872
Deubiquitination of p53 by HAUSP is an important pathway for p53 stabilization.
GO:0031625 ubiquitin protein ligase binding
IPI
PMID:8875929
Structure of the MDM2 oncoprotein bound to the p53 tumor sup... 		REMOVE
Summary: Generic 'ubiquitin protein ligase binding' is less informative than specific MDM2/MDM4 interactions.
Reason: While p53 binds MDM2 (E3 ligase), this generic term is uninformative. The specific MDM2 interaction is well-documented elsewhere.
Supporting Evidence:
PMID:8875929
Structure of the MDM2 oncoprotein bound to the p53 tumor suppressor transactivation domain.
GO:0051721 protein phosphatase 2A binding
IPI
PMID:17245430
A specific PP2A regulatory subunit, B56gamma, mediates DNA d... 		ACCEPT
Summary: p53 interacts with tubulin during mitosis and cell division control.
Reason: p53 regulates cell division and can interact with cytoskeletal components.
Supporting Evidence:
PMID:17245430
A specific PP2A regulatory subunit, B56gamma, mediates DNA damage-induced dephosphorylation of p53 at Thr55.
GO:0005634 nucleus
IDA
PMID:20096447
USP10 regulates p53 localization and stability by deubiquiti... 		ACCEPT
Summary: p53 is primarily a nuclear transcription factor, with nuclear localization essential for its function.
Reason: Core cellular component - p53's nuclear localization is essential for its transcription factor activity. Contains nuclear localization signals at residues 305-322 and 369-375.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Endoplasmic reticulum.
PMID:14963330
Direct activation of Bax by p53 mediates mitochondrial membrane permeabilization and apoptosis.
PMID:16322561
NIR is a novel INHAT repressor that modulates the transcriptional activity of p53.
PMID:36108750
Phase separation of p53 induced by its unstructured basic region and prevented by oncogenic mutations in tetramerization domain.
PMID:24625977
Ubiquitin-like (UBX)-domain-containing protein, UBXN2A, promotes cell death by interfering with the p53-Mortalin interactions in colon cancer cells.
PMID:26634371
Epub 2015 Dec 4. Structural studies of UBXN2A and mortalin interaction and the putative role of silenced UBXN2A in preventing response to chemotherapy.
PMID:21597459
2011 May 20. E3 ubiquitin ligase Hades negatively regulates the exonuclear function of p53.
PMID:19011621
Arginine methylation regulates the p53 response.
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:24101517
Mitochondrial disulfide relay mediates translocation of p53 and partitions its subcellular activity.
PMID:20810912
Hypoxia downregulates p53 but induces apoptosis and enhances expression of BAD in cultures of human syncytiotrophoblasts.
PMID:19234109
Induction of SOX4 by DNA damage is critical for p53 stabilization and function.
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:17403783
HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53.
PMID:20096447
USP10 regulates p53 localization and stability by deubiquitinating p53.
PMID:18756595
Prognostic significance of BMP and activin membrane-bound inhibitor in colorectal cancer.
PMID:16507995
Myosin VI is a mediator of the p53-dependent cell survival pathway.
PMID:7720704
Direct involvement of p53 in programmed cell death of oligodendrocytes.
GO:0005737 cytoplasm
IDA
PMID:20096447
USP10 regulates p53 localization and stability by deubiquiti... 		ACCEPT
Summary: p53 localizes to cytoplasm as well as nucleus, with cytoplasmic localization important for regulation.
Reason: Core cellular component - p53 shuttles between nucleus and cytoplasm, with cytoplasmic localization important for MDM2-mediated regulation.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus. Cytoplasm.
PMID:24625977
Ubiquitin-like (UBX)-domain-containing protein, UBXN2A, promotes cell death by interfering with the p53-Mortalin interactions in colon cancer cells.
PMID:26634371
Epub 2015 Dec 4. Structural studies of UBXN2A and mortalin interaction and the putative role of silenced UBXN2A in preventing response to chemotherapy.
PMID:21597459
2011 May 20. E3 ubiquitin ligase Hades negatively regulates the exonuclear function of p53.
PMID:19011621
Arginine methylation regulates the p53 response.
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:23629966
Deacetylation of p53 induces autophagy by suppressing Bmf expression.
PMID:20810912
Hypoxia downregulates p53 but induces apoptosis and enhances expression of BAD in cultures of human syncytiotrophoblasts.
PMID:16131611
p53 isoforms can regulate p53 transcriptional activity.
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:20096447
USP10 regulates p53 localization and stability by deubiquitinating p53.
PMID:7720704
Direct involvement of p53 in programmed cell death of oligodendrocytes.
GO:0045893 positive regulation of DNA-templated transcription
IMP
PMID:20096447
USP10 regulates p53 localization and stability by deubiquiti... 		ACCEPT
Summary: p53 positively regulates DNA-templated transcription of target genes.
Reason: Core transcriptional function - p53 activates transcription of numerous target genes.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:20378837
Glutaminase 2, a novel p53 target gene regulating energy metabolism and antioxidant function.
PMID:16322561
NIR is a novel INHAT repressor that modulates the transcriptional activity of p53.
PMID:17403783
HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53.
PMID:20096447
USP10 regulates p53 localization and stability by deubiquitinating p53.
GO:0032991 protein-containing complex
IDA
PMID:9529249
ARF promotes MDM2 degradation and stabilizes p53: ARF-INK4a ... 		ACCEPT
Summary: p53 forms part of protein-containing complexes including the p53 tetramer and complexes with co-activators.
Reason: p53 functions in complexes with CBP/p300, MDM2, and forms tetramers.
Supporting Evidence:
TP53-deep-research
Complex formation with transcriptional co-activators (CBP/p300, TFIID)
PMID:17310983
Feb 19. Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function.
PMID:15629713
Binding of natively unfolded HIF-1alpha ODD domain to p53.
PMID:9529249
ARF promotes MDM2 degradation and stabilizes p53: ARF-INK4a locus deletion impairs both the Rb and p53 tumor suppression pathways.
GO:0007265 Ras protein signal transduction
IEP
PMID:9054499
Oncogenic ras provokes premature cell senescence associated ... 		ACCEPT
Summary: p53 is regulated by Ras signaling pathway which can activate p53.
Reason: p53 is activated by oncogenic Ras signaling as a tumor suppressor mechanism.
Supporting Evidence:
PMID:9054499
Oncogenic ras provokes premature cell senescence associated with accumulation of p53 and p16INK4a.
GO:0005634 nucleus
IDA
PMID:18756595
Prognostic significance of BMP and activin membrane-bound in... 		ACCEPT
Summary: p53 is primarily a nuclear transcription factor, with nuclear localization essential for its function.
Reason: Core cellular component - p53's nuclear localization is essential for its transcription factor activity. Contains nuclear localization signals at residues 305-322 and 369-375.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Endoplasmic reticulum.
PMID:14963330
Direct activation of Bax by p53 mediates mitochondrial membrane permeabilization and apoptosis.
PMID:16322561
NIR is a novel INHAT repressor that modulates the transcriptional activity of p53.
PMID:36108750
Phase separation of p53 induced by its unstructured basic region and prevented by oncogenic mutations in tetramerization domain.
PMID:24625977
Ubiquitin-like (UBX)-domain-containing protein, UBXN2A, promotes cell death by interfering with the p53-Mortalin interactions in colon cancer cells.
PMID:26634371
Epub 2015 Dec 4. Structural studies of UBXN2A and mortalin interaction and the putative role of silenced UBXN2A in preventing response to chemotherapy.
PMID:21597459
2011 May 20. E3 ubiquitin ligase Hades negatively regulates the exonuclear function of p53.
PMID:19011621
Arginine methylation regulates the p53 response.
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:24101517
Mitochondrial disulfide relay mediates translocation of p53 and partitions its subcellular activity.
PMID:20810912
Hypoxia downregulates p53 but induces apoptosis and enhances expression of BAD in cultures of human syncytiotrophoblasts.
PMID:19234109
Induction of SOX4 by DNA damage is critical for p53 stabilization and function.
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:17403783
HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53.
PMID:20096447
USP10 regulates p53 localization and stability by deubiquitinating p53.
PMID:18756595
Prognostic significance of BMP and activin membrane-bound inhibitor in colorectal cancer.
PMID:16507995
Myosin VI is a mediator of the p53-dependent cell survival pathway.
PMID:7720704
Direct involvement of p53 in programmed cell death of oligodendrocytes.
GO:0010332 response to gamma radiation
IMP
PMID:7958916
DNA damage triggers a prolonged p53-dependent G1 arrest and ... 		ACCEPT
Summary: p53 responds to gamma radiation-induced DNA damage.
Reason: Core function - ionizing radiation strongly activates p53.
Supporting Evidence:
TP53-deep-research
p53 responds to DNA damage from ionizing radiation
PMID:7958916
DNA damage triggers a prolonged p53-dependent G1 arrest and long-term induction of Cip1 in normal human fibroblasts.
GO:0031571 mitotic G1 DNA damage checkpoint signaling
IMP
PMID:7958916
DNA damage triggers a prolonged p53-dependent G1 arrest and ... 		ACCEPT
Summary: p53 mediates G1 DNA damage checkpoint signaling through p21 induction.
Reason: Core function - G1/S checkpoint is a major p53-mediated cell cycle control.
Supporting Evidence:
TP53-deep-research
Cell cycle arrest is mediated by transcriptional upregulation of p21
PMID:7958916
DNA damage triggers a prolonged p53-dependent G1 arrest and long-term induction of Cip1 in normal human fibroblasts.
GO:0005515 protein binding
IPI
PMID:17317671
The notch regulator MAML1 interacts with p53 and functions a... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:17317671
2007 Feb 22. The notch regulator MAML1 interacts with p53 and functions as a coactivator.
GO:0005515 protein binding
IPI
PMID:19151705
CHD8 suppresses p53-mediated apoptosis through histone H1 re... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:19151705
CHD8 suppresses p53-mediated apoptosis through histone H1 recruitment during early embryogenesis.
GO:0008270 zinc ion binding
TAS
PMID:10065153
Covalent and noncovalent modifiers of the p53 protein. 		ACCEPT
Summary: p53 DNA-binding domain coordinates a zinc ion essential for structural stability.
Reason: Core structural feature - zinc coordination is essential for p53 DNA-binding domain structure.
Supporting Evidence:
TP53-deep-research
DNA-Binding Domain contains critical zinc coordination site
PMID:10065153
Covalent and noncovalent modifiers of the p53 protein.
GO:0016605 PML body
IDA
PMID:12006491
Human SIR2 deacetylates p53 and antagonizes PML/p53-induced ... 		ACCEPT
Summary: p53 localizes to PML nuclear bodies, which is important for its post-translational modification and activation.
Reason: Important regulatory localization - p53 accumulates in PML bodies during stress responses where it undergoes acetylation.
Supporting Evidence:
TP53-deep-research
p53 localizes to nuclear bodies like PML bodies, especially during stress responses
PMID:22869143
BMK1 is involved in the regulation of p53 through disrupting the PML-MDM2 interaction.
PMID:12006491
Human SIR2 deacetylates p53 and antagonizes PML/p53-induced cellular senescence.
GO:0051087 protein-folding chaperone binding
IPI
PMID:18086682
Hsp60 regulation of tumor cell apoptosis. 		KEEP AS NON CORE
Summary: p53 has chaperone binding activity related to its folding and stability.
Reason: Non-core function related to protein folding and quality control.
Supporting Evidence:
PMID:18086682
2007 Dec 17. Hsp60 regulation of tumor cell apoptosis.
GO:0003682 chromatin binding
IDA
PMID:17599062
CDIP, a novel pro-apoptotic gene, regulates TNFalpha-mediate... 		ACCEPT
Summary: p53 binds chromatin at p53 response elements in target gene promoters.
Reason: Core molecular function - chromatin binding is essential for p53's transcriptional regulation.
Supporting Evidence:
PDB:3TS8
Crystal structure of p53 tetramer bound to DNA
PMID:26334721
2015 Sep 3. Jmjd5 functions as a regulator of p53 signaling during mouse embryogenesis.
PMID:16322561
NIR is a novel INHAT repressor that modulates the transcriptional activity of p53.
PMID:17599062
Jun 28. CDIP, a novel pro-apoptotic gene, regulates TNFalpha-mediated apoptosis in a p53-dependent manner.
GO:0097193 intrinsic apoptotic signaling pathway
TAS
PMID:16462759
Inhibition of Bax activity is crucial for the antiapoptotic ... 		ACCEPT
Summary: p53 mediates intrinsic apoptotic signaling through mitochondrial pathway activation.
Reason: Core function - intrinsic apoptotic signaling is a major p53 tumor suppressor mechanism.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Apoptosis induction seems to be mediated either by stimulation of BAX
PMID:16462759
Feb 6. Inhibition of Bax activity is crucial for the antiapoptotic function of the human papillomavirus E6 oncoprotein.
GO:0005634 nucleus
IDA
PMID:16507995
Myosin VI is a mediator of the p53-dependent cell survival p... 		ACCEPT
Summary: p53 is primarily a nuclear transcription factor, with nuclear localization essential for its function.
Reason: Core cellular component - p53's nuclear localization is essential for its transcription factor activity. Contains nuclear localization signals at residues 305-322 and 369-375.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Endoplasmic reticulum.
PMID:14963330
Direct activation of Bax by p53 mediates mitochondrial membrane permeabilization and apoptosis.
PMID:16322561
NIR is a novel INHAT repressor that modulates the transcriptional activity of p53.
PMID:36108750
Phase separation of p53 induced by its unstructured basic region and prevented by oncogenic mutations in tetramerization domain.
PMID:24625977
Ubiquitin-like (UBX)-domain-containing protein, UBXN2A, promotes cell death by interfering with the p53-Mortalin interactions in colon cancer cells.
PMID:26634371
Epub 2015 Dec 4. Structural studies of UBXN2A and mortalin interaction and the putative role of silenced UBXN2A in preventing response to chemotherapy.
PMID:21597459
2011 May 20. E3 ubiquitin ligase Hades negatively regulates the exonuclear function of p53.
PMID:19011621
Arginine methylation regulates the p53 response.
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:24101517
Mitochondrial disulfide relay mediates translocation of p53 and partitions its subcellular activity.
PMID:20810912
Hypoxia downregulates p53 but induces apoptosis and enhances expression of BAD in cultures of human syncytiotrophoblasts.
PMID:19234109
Induction of SOX4 by DNA damage is critical for p53 stabilization and function.
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:17403783
HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53.
PMID:20096447
USP10 regulates p53 localization and stability by deubiquitinating p53.
PMID:18756595
Prognostic significance of BMP and activin membrane-bound inhibitor in colorectal cancer.
PMID:16507995
Myosin VI is a mediator of the p53-dependent cell survival pathway.
PMID:7720704
Direct involvement of p53 in programmed cell death of oligodendrocytes.
GO:0008104 intracellular protein localization
IDA
PMID:16507995
Myosin VI is a mediator of the p53-dependent cell survival p... 		ACCEPT
Summary: p53 localization is regulated by nuclear import and export mechanisms.
Reason: Core regulatory mechanism - nucleo-cytoplasmic shuttling regulates p53 activity.
Supporting Evidence:
TP53-deep-research
Nuclear Localization Signals (NLS): Function: Nuclear import and localization
PMID:16507995
Myosin VI is a mediator of the p53-dependent cell survival pathway.
GO:0046982 protein heterodimerization activity
IPI
PMID:10837489
MCL-1S, a splicing variant of the antiapoptotic BCL-2 family... 		ACCEPT
Summary: p53 binds to hetero-dimeric protein partners as part of its regulatory function.
Reason: p53 forms heterodimers with various regulatory proteins.
Supporting Evidence:
PMID:10837489
MCL-1S, a splicing variant of the antiapoptotic BCL-2 family member MCL-1, encodes a proapoptotic protein possessing only the BH3 domain.
GO:0005515 protein binding
IPI
PMID:15735003
p53CSV, a novel p53-inducible gene involved in the p53-depen... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:15735003
p53CSV, a novel p53-inducible gene involved in the p53-dependent cell-survival pathway.
GO:0005515 protein binding
IPI
PMID:11861836
Human immunodeficiency virus type 1 Nef binds to tumor suppr... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:11861836
Human immunodeficiency virus type 1 Nef binds to tumor suppressor p53 and protects cells against p53-mediated apoptosis.
GO:0005515 protein binding
IPI
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific P... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0005654 nucleoplasm
IDA
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific P... 		ACCEPT
Summary: p53 is a nuclear transcription factor that localizes to the nucleoplasm.
Reason: Core cellular localization - p53 functions as a transcription factor in the nucleoplasm.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0016363 nuclear matrix
IDA
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific P... 		ACCEPT
Summary: p53 regulates nuclear matrix organization.
Reason: p53 interacts with nuclear matrix components and influences nuclear organization.
Supporting Evidence:
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
GO:0065003 protein-containing complex assembly
IDA
PMID:12915590
Cellular stress and DNA damage invoke temporally distinct Md... 		ACCEPT
Summary: p53 forms protein-containing complexes with transcriptional machinery and regulators.
Reason: Core function - p53 assembles into functional complexes to regulate transcription.
Supporting Evidence:
TP53-deep-research
Complex formation with transcriptional co-activators (CBP/p300, TFIID)
PMID:12915590
Aug 12. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
GO:0005515 protein binding
IPI
PMID:11684014
ASPP proteins specifically stimulate the apoptotic function ... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:11684014
ASPP proteins specifically stimulate the apoptotic function of p53.
GO:0005515 protein binding
IPI
PMID:8675009
The XPB and XPD DNA helicases are components of the p53-medi... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:8675009
The XPB and XPD DNA helicases are components of the p53-mediated apoptosis pathway.
GO:0005515 protein binding
IPI
PMID:10608892
Stabilization of the MDM2 oncoprotein by interaction with th... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:10608892
Stabilization of the MDM2 oncoprotein by interaction with the structurally related MDMX protein.
GO:0005515 protein binding
IPI
PMID:12750254
p29ING4 and p28ING5 bind to p53 and p300, and enhance p53 ac... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:12750254
p29ING4 and p28ING5 bind to p53 and p300, and enhance p53 activity.
GO:0051097 negative regulation of helicase activity
TAS
PMID:7663514
p53 modulation of TFIIH-associated nucleotide excision repai... 		ACCEPT
Summary: p53 inhibits helicase activity as part of DNA repair regulation.
Reason: p53 can regulate DNA repair processes including helicase activity during damage response.
Supporting Evidence:
PMID:7663514
p53 modulation of TFIIH-associated nucleotide excision repair activity.
GO:0006289 nucleotide-excision repair
IMP
PMID:7663514
p53 modulation of TFIIH-associated nucleotide excision repai... 		ACCEPT
Summary: p53 promotes nucleotide-excision repair (NER) through transcriptional regulation of XPC and DDB2.
Reason: Core DNA repair function - p53 regulates multiple DNA repair genes including those in the NER pathway.
Supporting Evidence:
TP53-deep-research
DNA Repair: XPC: Nucleotide excision repair; DDB2: DNA damage-binding protein
PMID:7663514
p53 modulation of TFIIH-associated nucleotide excision repair activity.
GO:0005515 protein binding
IPI
PMID:1465435
Wild-type p53 binds to the TATA-binding protein and represse... 		REMOVE
Summary: Generic 'protein binding' term provides no functional information about p53's specific protein-protein interactions with MDM2, TFIID, CBP/p300, etc.
Reason: The term 'protein binding' is uninformative and should be removed per GO curation guidelines. p53 has many well-characterized functional protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better represented by more specific terms.
Supporting Evidence:
PMID:1465435
Wild-type p53 binds to the TATA-binding protein and represses transcription.
GO:0005739 mitochondrion
IDA
PMID:12667443
p53 has a direct apoptogenic role at the mitochondria. 		ACCEPT
Summary: p53 translocates to mitochondria to directly promote apoptosis through BAX activation.
Reason: Important localization for non-transcriptional apoptosis - p53 can directly induce MOMP at mitochondria.
Supporting Evidence:
TP53-deep-research
Non-transcriptional: Direct mitochondrial translocation and interaction with BCL-2 family proteins
PMID:25168243
Aug 29. Cofactor Strap regulates oxidative phosphorylation and mitochondrial p53 activity through ATP synthase.
PMID:24101517
Mitochondrial disulfide relay mediates translocation of p53 and partitions its subcellular activity.
PMID:12667443
p53 has a direct apoptogenic role at the mitochondria.
GO:0005507 copper ion binding
IDA
PMID:7824276
Modulation by copper of p53 conformation and sequence-specif... 		ACCEPT
Summary: p53 DNA-binding domain binds copper ions which can affect its function.
Reason: Structural feature - copper binding can affect p53 structure and function.
Supporting Evidence:
PMID:7824276
Modulation by copper of p53 conformation and sequence-specific DNA binding: role for Cu(II)/Cu(I) redox mechanism.
GO:0030308 negative regulation of cell growth
IMP
PMID:8986812
Identification of a novel p53 functional domain that is nece... 		ACCEPT
Summary: p53 negatively regulates cell growth as a fundamental tumor suppressor mechanism.
Reason: Core function - growth inhibition is a primary tumor suppressor activity of p53.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Negatively regulates cell division by controlling expression of a set of genes required for this process
PMID:8986812
Identification of a novel p53 functional domain that is necessary for efficient growth suppression.
GO:0005634 nucleus
IDA
PMID:7720704
Direct involvement of p53 in programmed cell death of oligod... 		ACCEPT
Summary: p53 is primarily a nuclear transcription factor, with nuclear localization essential for its function.
Reason: Core cellular component - p53's nuclear localization is essential for its transcription factor activity. Contains nuclear localization signals at residues 305-322 and 369-375.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Endoplasmic reticulum.
PMID:14963330
Direct activation of Bax by p53 mediates mitochondrial membrane permeabilization and apoptosis.
PMID:16322561
NIR is a novel INHAT repressor that modulates the transcriptional activity of p53.
PMID:36108750
Phase separation of p53 induced by its unstructured basic region and prevented by oncogenic mutations in tetramerization domain.
PMID:24625977
Ubiquitin-like (UBX)-domain-containing protein, UBXN2A, promotes cell death by interfering with the p53-Mortalin interactions in colon cancer cells.
PMID:26634371
Epub 2015 Dec 4. Structural studies of UBXN2A and mortalin interaction and the putative role of silenced UBXN2A in preventing response to chemotherapy.
PMID:21597459
2011 May 20. E3 ubiquitin ligase Hades negatively regulates the exonuclear function of p53.
PMID:19011621
Arginine methylation regulates the p53 response.
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:24289924
Nov 27. Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:24101517
Mitochondrial disulfide relay mediates translocation of p53 and partitions its subcellular activity.
PMID:20810912
Hypoxia downregulates p53 but induces apoptosis and enhances expression of BAD in cultures of human syncytiotrophoblasts.
PMID:19234109
Induction of SOX4 by DNA damage is critical for p53 stabilization and function.
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:17403783
HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53.
PMID:20096447
USP10 regulates p53 localization and stability by deubiquitinating p53.
PMID:18756595
Prognostic significance of BMP and activin membrane-bound inhibitor in colorectal cancer.
PMID:16507995
Myosin VI is a mediator of the p53-dependent cell survival pathway.
PMID:7720704
Direct involvement of p53 in programmed cell death of oligodendrocytes.
GO:0005737 cytoplasm
IDA
PMID:7720704
Direct involvement of p53 in programmed cell death of oligod... 		ACCEPT
Summary: p53 localizes to cytoplasm as well as nucleus, with cytoplasmic localization important for regulation.
Reason: Core cellular component - p53 shuttles between nucleus and cytoplasm, with cytoplasmic localization important for MDM2-mediated regulation.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
SUBCELLULAR LOCATION: Nucleus. Cytoplasm.
PMID:24625977
Ubiquitin-like (UBX)-domain-containing protein, UBXN2A, promotes cell death by interfering with the p53-Mortalin interactions in colon cancer cells.
PMID:26634371
Epub 2015 Dec 4. Structural studies of UBXN2A and mortalin interaction and the putative role of silenced UBXN2A in preventing response to chemotherapy.
PMID:21597459
2011 May 20. E3 ubiquitin ligase Hades negatively regulates the exonuclear function of p53.
PMID:19011621
Arginine methylation regulates the p53 response.
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:23629966
Deacetylation of p53 induces autophagy by suppressing Bmf expression.
PMID:20810912
Hypoxia downregulates p53 but induces apoptosis and enhances expression of BAD in cultures of human syncytiotrophoblasts.
PMID:16131611
p53 isoforms can regulate p53 transcriptional activity.
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:20096447
USP10 regulates p53 localization and stability by deubiquitinating p53.
PMID:7720704
Direct involvement of p53 in programmed cell death of oligodendrocytes.
GO:0003677 DNA binding
IMP
PMID:2144364
Transcriptional activation by wild-type but not transforming... 		ACCEPT
Summary: p53 binds DNA through its central DNA-binding domain (residues 102-292) to p53 response elements.
Reason: Core molecular function - DNA binding is essential for p53's transcription factor activity.
Supporting Evidence:
TP53-deep-research
DNA-Binding Domain (DBD): Location: Residues 102-292; Structure: beta-sandwich core with DNA-binding loops
PMID:15358771
2004 Sep 9. Hsp90 regulates the activity of wild type p53 under physiological and elevated temperatures.
PMID:15629713
Binding of natively unfolded HIF-1alpha ODD domain to p53.
PMID:2144364
Transcriptional activation by wild-type but not transforming mutants of the p53 anti-oncogene.
GO:0003700 DNA-binding transcription factor activity
IDA
PMID:7587074
Targets for transcriptional activation by wild-type p53: end... 		ACCEPT
Summary: p53 is a well-characterized DNA-binding transcription factor that activates and represses target genes.
Reason: Core molecular function - p53 is one of the best-characterized transcription factors, regulating >500 target genes.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:26334721
2015 Sep 3. Jmjd5 functions as a regulator of p53 signaling during mouse embryogenesis.
PMID:18549481
TATA binding protein associated factor 3 (TAF3) interacts with p53 and inhibits its function.
PMID:7587074
Targets for transcriptional activation by wild-type p53: endogenous retroviral LTR, immunoglobulin-like promoter, and an internal promoter of the mdm2 gene.
GO:0005730 nucleolus
IDA
PMID:12080348
Nucleophosmin regulates the stability and transcriptional ac... 		ACCEPT
Summary: p53 localizes to nucleolus, particularly during stress responses and ribosome biogenesis regulation.
Reason: p53 can localize to nucleolus and regulate ribosomal biogenesis as part of stress response.
Supporting Evidence:
PMID:12080348
Nucleophosmin regulates the stability and transcriptional activity of p53.
GO:0006355 regulation of DNA-templated transcription
IDA
PMID:7587074
Targets for transcriptional activation by wild-type p53: end... 		ACCEPT
Summary: p53 regulates DNA-templated transcription as both an activator and repressor.
Reason: Core molecular function - transcription regulation is fundamental to p53's tumor suppressor activity.
Supporting Evidence:
file:human/TP53/TP53-uniprot.txt
Multifunctional transcription factor
PMID:25417702
Functional interplay between MDM2, p63/p73 and mutant p53.
PMID:7587074
Targets for transcriptional activation by wild-type p53: endogenous retroviral LTR, immunoglobulin-like promoter, and an internal promoter of the mdm2 gene.

Core Functions

p53 functions as a sequence-specific DNA-binding transcription factor that binds p53 response elements to regulate transcription of target genes involved in cell cycle arrest, apoptosis, DNA repair, and metabolism

Molecular Function:
transcription cis-regulatory region binding
Directly Involved In:
DNA damage response, signal transduction by p53 class mediator negative regulation of mitotic cell cycle intrinsic apoptotic signaling pathway by p53 class mediator
Cellular Locations:
nucleus
Supporting Evidence:
  • PMID:15710329
    Human MUC1 oncoprotein regulates p53-responsive gene transcription
  • PMID:17996705
    An acetylation switch in p53 mediates holo-TFIID recruitment
  • file:human/TP53/TP53-uniprot.txt
    Multifunctional transcription factor

p53 localizes to nuclear bodies and regulates both activation and repression of transcription, including senescence programs

Molecular Function:
transcription cis-regulatory region binding
Directly Involved In:
positive regulation of transcription by RNA polymerase II negative regulation of transcription by RNA polymerase II regulation of cellular senescence
Cellular Locations:
nuclear body
Supporting Evidence:
  • PMID:10360174
    ARF exon 2 disrupt its nucleolar localization and impair its ability to block nuclear export of MDM2 and p53
  • file:human/TP53/TP53-uniprot.txt
    induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence ... Negatively regulates cell division

References

PDB:3EXJ
Crystal Structure of a p53 Core Tetramer Bound to DNA
  • p53 core tetramer forms symmetric contacts with DNA through two p53 dimers
    "The structure reveals that two p53DBD dimers bind to B form DNA with no relative twist and that a p53 tetramer can bind to DNA without introducing significant DNA bending"
  • p53 DNA binding involves conformational changes in loop L1 of two subunits
    "In the absence of DNA, loop L1 of the p53 DNA binding domain adopts an extended conformation, whereas two p53 subunits switch to a recessed loop L1 conformation when bound to DNA as a tetramer"
  • The structure demonstrates cooperative self-assembly of p53 on DNA response elements
    "DNA binding by the p53 core domain is a cooperative self-assembling process accompanied by structural changes of the p53 dimer and DNA"
PDB:1C26
Crystal Structure of p53 Tetramerization Domain
  • The tetramerization domain forms a dimer-of-dimers architecture stabilizing the p53 tetramer
    "The monomer consists of a beta strand and an alpha helix, associates with a second monomer across an antiparallel beta sheet and an antiparallel helix-helix interface to form a dimer"
  • Tetramerization domain structure reveals the molecular basis for p53 oligomerization
    "Crystal structure of p53 tetramerization domain (residues 325 to 356) at 1.7 angstrom resolution"
PDB:3TS8
Crystal structure of a multidomain human p53 tetramer bound to the natural CDKN1A(p21) p53-response element
  • Full-length p53 tetramer structure shows both DNA-binding and tetramerization domains in context
    "Crystal structure of a multidomain human p53 tetramer encompassing both the DNA-binding and homo-oligomerization domains in complex with the natural p53-response element present upstream of the CDKN1A (p21) gene promoter"
  • The structure demonstrates p53 binding to natural response elements in target gene promoters
    "The structure demonstrates how p53 recognizes and binds to its natural response element in the CDKN1A (p21) gene, a key cell cycle checkpoint target"
GO_REF:0000002
Gene Ontology annotation through association of InterPro records with GO terms.
GO_REF:0000107
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara.
GO_REF:0000120
Combined Automated Annotation using Multiple IEA Methods.
PMID:10360174
Mutations in human ARF exon 2 disrupt its nucleolar localization and impair its ability to block nuclear export of MDM2 and p53.
PMID:10962037
Overexpression of MYC causes p53-dependent G2 arrest of normal fibroblasts.
PMID:14654789
A member of the Pyrin family, IFI16, is a novel BRCA1-associated protein involved in the p53-mediated apoptosis pathway.
PMID:14744935
Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.
PMID:15710329
Human MUC1 oncoprotein regulates p53-responsive gene transcription in the genotoxic stress response.
PMID:16061649
Ckap2 regulates aneuploidy, cell cycling, and cell death in a p53-dependent manner.
PMID:17599062
CDIP, a novel pro-apoptotic gene, regulates TNFalpha-mediated apoptosis in a p53-dependent manner.
PMID:17996705
An acetylation switch in p53 mediates holo-TFIID recruitment.
PMID:30089260
p53 Regulates the Expression of LRP1 and Apoptosis through a Stress Intensity-Dependent MicroRNA Feedback Loop.
PMID:30514107
CELF1/p53 axis: a sustained antiproliferative signal leading to villus atrophy under total parenteral nutrition.
file:human/TP53/TP53-uniprot.txt
UniProt entry for Human TP53
file:human/TP53/TP53-deep-research.md
TP53 Comprehensive Deep Research
GO_REF:0000024
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
GO_REF:0000033
Annotation inferences using phylogenetic trees
GO_REF:0000043
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
GO_REF:0000044
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt.
GO_REF:0000052
Gene Ontology annotation based on curation of immunofluorescence data
GO_REF:0000113
Gene Ontology annotation of human sequence-specific DNA binding transcription factors (DbTFs) based on the TFClass database
GO_REF:0000117
Electronic Gene Ontology annotations created by ARBA machine learning models
PMID:10065153
Covalent and noncovalent modifiers of the p53 protein.
PMID:10196247
Characterization of an E1A-CBP interaction defines a novel transcriptional adapter motif (TRAM) in CBP/p300.
PMID:10226625
The yeast two-hybrid system reveals no interaction between p73 alpha and SV40 large T-antigen.
PMID:10329733
p53 suppresses the activation of the Bcl-2 promoter by the Brn-3a POU family transcription factor.
PMID:10415337
Identification of a novel gene encoding a p53-associated protein.
PMID:10518217
A novel cofactor for p300 that regulates the p53 response.
PMID:10597287
Molecular interactions between telomerase and the tumor suppressor protein p53 in vitro.
PMID:10608892
Stabilization of the MDM2 oncoprotein by interaction with the structurally related MDMX protein.
PMID:10666337
Human topoisomerase IIalpha and IIbeta interact with the C-terminal region of p53.
PMID:10823891
The Huntington's disease protein interacts with p53 and CREB-binding protein and represses transcription.
PMID:10837489
MCL-1S, a splicing variant of the antiapoptotic BCL-2 family member MCL-1, encodes a proapoptotic protein possessing only the BH3 domain.
PMID:10876243
Structure of the negative regulatory domain of p53 bound to S100B(betabeta).
PMID:11080164
Regulation of p53 activity in nuclear bodies by a specific PML isoform.
PMID:11146555
Functional interaction between p53 and the interferon-inducible nucleoprotein IFI 16.
PMID:11178989
MDM2 enhances the function of estrogen receptor alpha in human breast cancer cells.
PMID:11359905
The corepressor mSin3a interacts with the proline-rich domain of p53 and protects p53 from proteasome-mediated degradation.
PMID:11388671
A putative protein inhibitor of activated STAT (PIASy) interacts with p53 and inhibits p53-mediated transactivation but not apoptosis.
PMID:11427532
p53 Modulates the exonuclease activity of Werner syndrome protein.
PMID:11546806
Cloning and characterization of a p53-related protein kinase expressed in interleukin-2-activated cytotoxic T-cells, epithelial tumor cell lines, and the testes.
PMID:11672523
hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase.
PMID:11684014
ASPP proteins specifically stimulate the apoptotic function of p53.
PMID:11706030
Differential effect of ik3-1/cables on p53- and p73-induced cell death.
PMID:11781842
The Bloom syndrome protein interacts and cooperates with p53 in regulation of transcription and cell growth control.
PMID:11861836
Human immunodeficiency virus type 1 Nef binds to tumor suppressor p53 and protects cells against p53-mediated apoptosis.
PMID:11877378
Structure of the 53BP1 BRCT region bound to p53 and its comparison to the Brca1 BRCT structure.
PMID:11923872
Deubiquitination of p53 by HAUSP is an important pathway for p53 stabilization.
PMID:11950834
SWI/SNF complex interacts with tumor suppressor p53 and is necessary for the activation of p53-mediated transcription.
PMID:12006491
Human SIR2 deacetylates p53 and antagonizes PML/p53-induced cellular senescence.
PMID:12080348
Nucleophosmin regulates the stability and transcriptional activity of p53.
PMID:12172011
Chk2 regulates irradiation-induced, p53-mediated apoptosis in Drosophila.
PMID:12203124
The Brn-3a transcription factor inhibits the pro-apoptotic effect of p53 and enhances cell cycle arrest by differentially regulating the activity of the p53 target genes encoding Bax and p21(CIP1/Waf1).
PMID:12433990
Distinct promoter elements mediate the co-operative effect of Brn-3a and p53 on the p21 promoter and their antagonism on the Bax promoter.
PMID:12609999
The activation domains, the proline-rich domain, and the C-terminal basic domain in p53 are necessary for acetylation of histones on the proximal p21 promoter and interaction with p300/CREB-binding protein.
PMID:12620801
Requirement of E6AP and the features of human papillomavirus E6 necessary to support degradation of p53.
PMID:12667443
p53 has a direct apoptogenic role at the mitochondria.
PMID:12692135
P63 alpha mutations lead to aberrant splicing of keratinocyte growth factor receptor in the Hay-Wells syndrome.
PMID:12702766
Characterization of cells and gene-targeted mice deficient for the p53-binding kinase homeodomain-interacting protein kinase 1 (HIPK1).
PMID:12730672
Physical and functional interaction between HCV core protein and the different p73 isoforms.
PMID:12750254
p29ING4 and p28ING5 bind to p53 and p300, and enhance p53 activity.
PMID:12915590
Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization.
PMID:14557665
Adenovirus E1B 55-kilodalton oncoprotein binds to Daxx and eliminates enhancement of p53-dependent transcription by Daxx.
PMID:14627987
PARP-1 binds E2F-1 independently of its DNA binding and catalytic domains, and acts as a novel coactivator of E2F-1-mediated transcription during re-entry of quiescent cells into S phase.
PMID:1465435
Wild-type p53 binds to the TATA-binding protein and represses transcription.
PMID:14759370
Structural mechanism of the bromodomain of the coactivator CBP in p53 transcriptional activation.
PMID:14963330
Direct activation of Bax by p53 mediates mitochondrial membrane permeabilization and apoptosis.
PMID:14985081
Hepatitis C virus core protein interacts with p53-binding protein, 53BP2/Bbp/ASPP2, and inhibits p53-mediated apoptosis.
PMID:15044383
Aprataxin, a novel protein that protects against genotoxic stress.
PMID:15053879
Phosphorylation on Thr-55 by TAF1 mediates degradation of p53: a role for TAF1 in cell G1 progression.
PMID:15068796
C. elegans SGK-1 is the critical component in the Akt/PKB kinase complex to control stress response and life span.
PMID:15133049
NMR structure of the N-terminal domain of SUMO ligase PIAS1 and its interaction with tumor suppressor p53 and A/T-rich DNA oligomers.
PMID:15144954
Nucleolar protein NPM interacts with HDM2 and protects tumor suppressor protein p53 from HDM2-mediated degradation.
PMID:15149599
Telomere shortening triggers senescence of human cells through a pathway involving ATM, p53, and p21(CIP1), but not p16(INK4a).
PMID:15205477
Calorie restriction promotes mammalian cell survival by inducing the SIRT1 deacetylase.
PMID:15310821
Identification of ASK and clock-associated proteins as molecular partners of LKP2 (LOV kelch protein 2) in Arabidopsis.
PMID:15314173
Ribosomal protein L23 activates p53 by inhibiting MDM2 function in response to ribosomal perturbation but not to translation inhibition.
PMID:15358771
Hsp90 regulates the activity of wild type p53 under physiological and elevated temperatures.
PMID:15364927
Negative regulation of p53 functions by Daxx and the involvement of MDM2.
PMID:15525938
Regulation of p53 activity through lysine methylation.
PMID:15542844
p53 Stabilization and accumulation induced by human vaccinia-related kinase 1.
PMID:15565177
A novel mitochondrial protein DIP mediates E2F1-induced apoptosis independently of p53.
PMID:15577914
Regulation of cellular response to oncogenic and oxidative stress by Seladin-1.
PMID:15580310
17beta-Estradiol upregulates and activates WOX1/WWOXv1 and WOX2/WWOXv2 in vitro: potential role in cancerous progression of breast and prostate to a premetastatic state in vivo.
PMID:15604276
Adenoviral E1A targets Mdm4 to stabilize tumor suppressor p53.
PMID:15629713
Binding of natively unfolded HIF-1alpha ODD domain to p53.
PMID:15660129
The DEAD box protein p68: a novel transcriptional coactivator of the p53 tumour suppressor.
PMID:15687255
A mechanism of ubiquitin-independent proteasomal degradation of the tumor suppressors p53 and p73.
PMID:15705871
Homeobox Msx1 interacts with p53 tumor suppressor and inhibits tumor growth by inducing apoptosis.
PMID:15735003
p53CSV, a novel p53-inducible gene involved in the p53-dependent cell-survival pathway.
PMID:15735006
p53 modulates RPA-dependent and RPA-independent WRN helicase activity.
PMID:15782130
BARD1 induces apoptosis by catalysing phosphorylation of p53 by DNA-damage response kinase.
PMID:15855171
Direct interaction of the N-terminal domain of focal adhesion kinase with the N-terminal transactivation domain of p53.
PMID:15916963
Loss of HAUSP-mediated deubiquitination contributes to DNA damage-induced destabilization of Hdmx and Hdm2.
PMID:15960975
Physical association and coordinate function of the H3 K4 methyltransferase MLL1 and the H4 K16 acetyltransferase MOF.
PMID:15989956
ARF-BP1/Mule is a critical mediator of the ARF tumor suppressor.
PMID:16003391
AtBAG6, a novel calmodulin-binding protein, induces programmed cell death in yeast and plants.
PMID:16131611
p53 isoforms can regulate p53 transcriptional activity.
PMID:16151013
PUMA couples the nuclear and cytoplasmic proapoptotic function of p53.
PMID:16169070
A human protein-protein interaction network: a resource for annotating the proteome.
PMID:16189514
Towards a proteome-scale map of the human protein-protein interaction network.
PMID:16213212
Regulation of p53 translation and induction after DNA damage by ribosomal protein L26 and nucleolin.
PMID:16227626
Physical interaction and mutual transrepression between CCAAT/enhancer-binding protein beta and the p53 tumor suppressor.
PMID:16291740
Charge modification at multiple C-terminal lysine residues regulates p53 oligomerization and its nucleus-cytoplasm trafficking.
PMID:16319068
Camptothecin induces nuclear export of prohibitin preferentially in transformed cells through a CRM-1-dependent mechanism.
PMID:16322561
NIR is a novel INHAT repressor that modulates the transcriptional activity of p53.
PMID:16376338
Protein kinase A phosphorylates and regulates dimerization of 14-3-3 epsilon.
PMID:16376884
Characterisation of the interface between nucleophosmin (NPM) and p53: potential role in p53 stabilisation.
PMID:16377624
Protein kinase C delta regulates Ser46 phosphorylation of p53 tumor suppressor in the apoptotic response to DNA damage.
PMID:16402859
Structural basis of competitive recognition of p53 and MDM2 by HAUSP/USP7: implications for the regulation of the p53-MDM2 pathway.
PMID:16415881
Structural basis for the methylation site specificity of SET7/9.
PMID:16432196
The central region of HDM2 provides a second binding site for p53.
PMID:16442532
Interaction of metallothionein with tumor suppressor p53 protein.
PMID:16443602
WT p53, but not tumor-derived mutants, bind to Bcl2 via the DNA binding domain and induce mitochondrial permeabilization.
PMID:16461914
Core domain interactions in full-length p53 in solution.
PMID:16462759
Inhibition of Bax activity is crucial for the antiapoptotic function of the human papillomavirus E6 oncoprotein.
PMID:16474402
Molecular recognition of p53 and MDM2 by USP7/HAUSP.
PMID:16479015
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2.
PMID:16492744
Mdm4 and Mdm2 cooperate to inhibit p53 activity in proliferating and quiescent cells in vivo.
PMID:16493710
Surface plasmon resonance imaging protein arrays for analysis of triple protein interactions of HPV, E6, E6AP, and p53.
PMID:16507995
Myosin VI is a mediator of the p53-dependent cell survival pathway.
PMID:16511572
14-3-3gamma binds to MDMX that is phosphorylated by UV-activated Chk1, resulting in p53 activation.
PMID:16601686
Tip60 and p400 are both required for UV-induced apoptosis but play antagonistic roles in cell cycle progression.
PMID:16611888
Targeting of p300/CREB binding protein coactivators by simian virus 40 is mediated through p53.
PMID:16616141
Evidence that low doses of Taxol enhance the functional transactivatory properties of p53 on p21 waf promoter in MCF-7 breast cancer cells.
PMID:16690937
NS3 protein of Hepatitis C virus associates with the tumour suppressor p53 and inhibits its function in an NS3 sequence-dependent manner.
PMID:16713569
A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration.
PMID:16732283
Lysine activation and functional analysis of E2-mediated conjugation in the SUMO pathway.
PMID:16753148
Polo-like kinase 1 regulates mitotic arrest after UV irradiation through dephosphorylation of p53 and inducing p53 degradation.
PMID:16793543
Structure of the Tfb1/p53 complex: Insights into the interaction between the p62/Tfb1 subunit of TFIIH and the activation domain of p53.
PMID:16845383
Critical role for Daxx in regulating Mdm2.
PMID:16847267
MAGE-A tumor antigens target p53 transactivation function through histone deacetylase recruitment and confer resistance to chemotherapeutic agents.
PMID:16951253
Crystal structure of SV40 large T-antigen bound to p53: interplay between a viral oncoprotein and a cellular tumor suppressor.
PMID:16959611
Gain of function of mutant p53: the mutant p53/NF-Y protein complex reveals an aberrant transcriptional mechanism of cell cycle regulation.
PMID:17080083
Inactivation of the p53 pathway in retinoblastoma.
PMID:17098746
FBXO11 promotes the Neddylation of p53 and inhibits its transcriptional activity.
PMID:17108971
Repression of p53 activity by Smyd2-mediated methylation.
PMID:17121812
CARPs are ubiquitin ligases that promote MDM2-independent p53 and phospho-p53ser20 degradation.
PMID:17139261
p53 mediates the negative regulation of MDM2 by orphan receptor TR3.
PMID:17145718
Brn-3b enhances the pro-apoptotic effects of p53 but not its induction of cell cycle arrest by cooperating in trans-activation of bax expression.
PMID:17146433
RGC32, a novel p53-inducible gene, is located on centrosomes during mitosis and results in G2/M arrest.
PMID:17159902
An essential function of the extreme C-terminus of MDM2 can be provided by MDMX.
PMID:17170702
Cytoplasmic destruction of p53 by the endoplasmic reticulum-resident ubiquitin ligase 'Synoviolin'.
PMID:17184779
Identification of differential proteins in nasopharyngeal carcinoma cells with p53 silence by proteome analysis.
PMID:17237821
Transcription factor TAFII250 promotes Mdm2-dependent turnover of p53.
PMID:17245430
A specific PP2A regulatory subunit, B56gamma, mediates DNA damage-induced dephosphorylation of p53 at Thr55.
PMID:17254968
PRAK is essential for ras-induced senescence and tumor suppression.
PMID:17268548
Monoubiquitylation promotes mitochondrial p53 translocation.
PMID:17274640
A limited screen for protein interactions reveals new roles for protein phosphatase 1 in cell cycle control and apoptosis.
PMID:17290220
The deubiquitinating enzyme USP2a regulates the p53 pathway by targeting Mdm2.
PMID:17298945
The conserved CPH domains of Cul7 and PARC are protein-protein interaction modules that bind the tetramerization domain of p53.
PMID:17310983
Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function.
PMID:17317671
The notch regulator MAML1 interacts with p53 and functions as a coactivator.
PMID:17347673
Stra13 is induced by genotoxic stress and regulates ionizing-radiation-induced apoptosis.
PMID:17403783
HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53.
PMID:17438265
Four domains of p300 each bind tightly to a sequence spanning both transactivation subdomains of p53.
PMID:17470788
PACT is a negative regulator of p53 and essential for cell growth and embryonic development.
PMID:17482142
Attenuation of DNA damage checkpoint by PBK, a novel mitotic kinase, involves protein-protein interaction with tumor suppressor p53.
PMID:17568776
Phosphorylation of Pirh2 by calmodulin-dependent kinase II impairs its ability to ubiquitinate p53.
PMID:17612295
Yeast split-ubiquitin-based cytosolic screening system to detect interactions between transcriptionally active proteins.
PMID:17620598
Quaternary structures of tumor suppressor p53 and a specific p53 DNA complex.
PMID:17662718
Protein-protein interactions among human lens acidic and basic beta-crystallins.
PMID:17707234
Modulation of p53 function by SET8-mediated methylation at lysine 382.
PMID:17719541
Hzf Determines cell survival upon genotoxic stress by modulating p53 transactivation.
PMID:17719542
hCAS/CSE1L associates with chromatin and regulates expression of select p53 target genes.
PMID:17805299
p53 is regulated by the lysine demethylase LSD1.
PMID:17875722
Efficient p53 activation and apoptosis by simultaneous disruption of binding to MDM2 and MDMX.
PMID:17904127
SVH-B interacts directly with p53 and suppresses the transcriptional activity of p53.
PMID:17906639
The prolyl isomerase Pin1 orchestrates p53 acetylation and dissociation from the apoptosis inhibitor iASPP.
PMID:17938203
Protein kinase C delta induces transcription of the TP53 tumor suppressor gene by controlling death-promoting factor Btf in the apoptotic response to DNA damage.
PMID:17964266
Active regulator of SIRT1 cooperates with SIRT1 and facilitates suppression of p53 activity.
PMID:18086682
Hsp60 regulation of tumor cell apoptosis.
PMID:18087040
Regulation of p53 tetramerization and nuclear export by ARC.
PMID:18172499
NUMB controls p53 tumour suppressor activity.
PMID:18230339
Activation of p53-dependent responses in tumor cells treated with a PARC-interacting peptide.
PMID:18235501
DBC1 is a negative regulator of SIRT1.
PMID:18235502
Negative regulation of the deacetylase SIRT1 by DBC1.
PMID:18275817
Biochemical and structural studies of ASPP proteins reveal differential binding to p53, p63, and p73.
PMID:18309296
Proteasome activator PA28 gamma regulates p53 by enhancing its MDM2-mediated degradation.
PMID:18316739
Temporal activation of p53 by a specific MDM2 inhibitor is selectively toxic to tumors and leads to complete tumor growth inhibition.
PMID:18354501
Structural insight into the TFIIE-TFIIH interaction: TFIIE and p53 share the binding region on TFIIH.
PMID:18382127
CARPs enhance p53 turnover by degrading 14-3-3sigma and stabilizing MDM2.
PMID:18388957
NAD(P)H quinone oxidoreductase 1 inhibits the proteasomal degradation of the tumour suppressor p33(ING1b).
PMID:18391200
Structure of tumor suppressor p53 and its intrinsically disordered N-terminal transactivation domain.
PMID:18485870
Acetylation is indispensable for p53 activation.
PMID:18504427
Twist and p53 reciprocally regulate target genes via direct interaction.
PMID:18510931
Ectodermal factor restricts mesoderm differentiation by inhibiting p53.
PMID:18549481
TATA binding protein associated factor 3 (TAF3) interacts with p53 and inhibits its function.
PMID:18566590
The tumour suppressor RASSF1A promotes MDM2 self-ubiquitination by disrupting the MDM2-DAXX-HAUSP complex.
PMID:18624398
Protein interaction data set highlighted with human Ras-MAPK/PI3K signaling pathways.
PMID:18656471
Microtubule-associated protein 1B light chain (MAP1B-LC1) negatively regulates the activity of tumor suppressor p53 in neuroblastoma cells.
PMID:18677113
Structure of the human Mdmx protein bound to the p53 tumor suppressor transactivation domain.
PMID:18690848
NUPR1 interacts with p53, transcriptionally regulates p21 and rescues breast epithelial cells from doxorubicin-induced genotoxic stress.
PMID:18695251
AIMP2/p38, the scaffold for the multi-tRNA synthetase complex, responds to genotoxic stresses via p53.
PMID:18756595
Prognostic significance of BMP and activin membrane-bound inhibitor in colorectal cancer.
PMID:18812399
14-3-3 activation of DNA binding of p53 by enhancing its association into tetramers.
PMID:18952844
Inhibition of Thr-55 phosphorylation restores p53 nuclear localization and sensitizes cancer cells to DNA damage.
PMID:18977328
Inactivation of the CYLD deubiquitinase by HPV E6 mediates hypoxia-induced NF-kappaB activation.
PMID:19008854
Interferon-inducible protein, P56, inhibits HPV DNA replication by binding to the viral protein E1.
PMID:19011621
Arginine methylation regulates the p53 response.
PMID:19043414
Molecular basis of Pirh2-mediated p53 ubiquitylation.
PMID:19098711
RYBP stabilizes p53 by modulating MDM2.
PMID:19151705
CHD8 suppresses p53-mediated apoptosis through histone H1 recruitment during early embryogenesis.
PMID:19160485
A ribosomal protein L23-nucleophosmin circuit coordinates Mizl function with cell growth.
PMID:19166840
FKBP25, a novel regulator of the p53 pathway, induces the degradation of MDM2 and activation of p53.
PMID:19196987
Phosphorylation of p53 by IkappaB kinase 2 promotes its degradation by beta-TrCP.
PMID:19217391
Structural basis for p300 Taz2-p53 TAD1 binding and modulation by phosphorylation.
PMID:19234109
Induction of SOX4 by DNA damage is critical for p53 stabilization and function.
PMID:19255450
Structural basis for high-affinity peptide inhibition of p53 interactions with MDM2 and MDMX.
PMID:19339993
Crosstalk between sumoylation and acetylation regulates p53-dependent chromatin transcription and DNA binding.
PMID:19345189
A Mutant-p53/Smad complex opposes p63 to empower TGFbeta-induced metastasis.
PMID:19411066
Regulation of XIAP translation and induction by MDM2 following irradiation.
PMID:19433796
A tumor suppressive coactivator complex of p53 containing ASC-2 and histone H3-lysine-4 methyltransferase MLL3 or its paralogue MLL4.
PMID:19483087
Identification and characterization of two novel isoforms of Pirh2 ubiquitin ligase that negatively regulate p53 independent of RING finger domains.
PMID:19505873
Complementary quantitative proteomics reveals that transcription factor AP-4 mediates E-box-dependent complex formation for transcriptional repression of HDM2.
PMID:19508870
TOE1 interacts with p53 to modulate its transactivation potential.
PMID:19521340
MDM4 (MDMX) localizes at the mitochondria and facilitates the p53-mediated intrinsic-apoptotic pathway.
PMID:19536131
Differential regulation of p53 and p21 by MKRN1 E3 ligase controls cell cycle arrest and apoptosis.
PMID:19556538
Trim24 targets endogenous p53 for degradation.
PMID:19619542
Mdmx enhances p53 ubiquitination by altering the substrate preference of the Mdm2 ubiquitin ligase.
PMID:19626115
Modulation of microRNA processing by p53.
PMID:19651603
Structural basis for subversion of cellular control mechanisms by the adenoviral E1A oncoprotein.
PMID:19656744
Ribosomal protein S3: A multi-functional protein that interacts with both p53 and MDM2 through its KH domain.
PMID:19667193
Ultraslow oligomerization equilibria of p53 and its implications.
PMID:19680552
CK2 is the regulator of SIRT1 substrate-binding affinity, deacetylase activity and cellular response to DNA-damage.
PMID:19684601
A molecular basis for phosphorylation-dependent SUMO conjugation by the E2 UBC9.
PMID:19740107
Epidermal growth factor receptor ligands as new extracellular targets for the metastasis-promoting S100A4 protein.
PMID:19749791
Repression of SHP-1 expression by p53 leads to trkA tyrosine phosphorylation and suppression of breast cancer cell proliferation.
PMID:19798103
Efficient protection and isolation of ubiquitylated proteins using tandem ubiquitin-binding entities.
PMID:19805293
CBP and p300 are cytoplasmic E4 polyubiquitin ligases for p53.
PMID:19833129
Polo-like kinase-1 phosphorylates MDM2 at Ser260 and stimulates MDM2-mediated p53 turnover.
PMID:19857493
Role of p53/FAK association and p53Ser46 phosphorylation in staurosporine-mediated apoptosis: wild type versus mutant p53-R175H.
PMID:19933256
Mechanistic differences in the transcriptional activation of p53 by 14-3-3 isoforms.
PMID:19951988
WNT16B is a new marker of cellular senescence that regulates p53 activity and the phosphoinositide 3-kinase/AKT pathway.
PMID:20004160
Averaging of electron subtomograms and random conical tilt reconstructions through likelihood optimization.
PMID:20075864
Coordinated regulation of p53 apoptotic targets BAX and PUMA by SMAR1 through an identical MAR element.
PMID:20096447
USP10 regulates p53 localization and stability by deubiquitinating p53.
PMID:20118233
G9a and Glp methylate lysine 373 in the tumor suppressor p53.
PMID:20123734
NIR, an inhibitor of histone acetyltransferases, regulates transcription factor TAp63 and is controlled by the cell cycle.
PMID:20124405
G-protein-coupled receptor kinase 5 phosphorylates p53 and inhibits DNA damage-induced apoptosis.
PMID:20134482
Skp2B attenuates p53 function by inhibiting prohibitin.
PMID:20153329
Reconstitution of the mitochondrial Hsp70 (mortalin)-p53 interaction using purified proteins--identification of additional interacting regions.
PMID:20159018
Identification and characterization of the novel protein CCDC106 that interacts with p53 and promotes its degradation.
PMID:20159469
Crystal structure of the p53 core domain bound to a full consensus site as a self-assembled tetramer.
PMID:20160708
Feedback between p21 and reactive oxygen production is necessary for cell senescence.
PMID:20167603
DYRK1A and DYRK3 promote cell survival through phosphorylation and activation of SIRT1.
PMID:20173098
RFWD3-Mdm2 ubiquitin ligase complex positively regulates p53 stability in response to DNA damage.
PMID:20206173
Structure of the p53 C-terminus bound to 14-3-3: implications for stabilization of the p53 tetramer.
PMID:20227041
Modulation of the vitamin D3 response by cancer-associated mutant p53.
PMID:20228809
BRD7 is a candidate tumour suppressor gene required for p53 function.
PMID:20332243
MicroRNA145 targets BNIP3 and suppresses prostate cancer progression.
PMID:20364130
Diversity in DNA recognition by p53 revealed by crystal structures with Hoogsteen base pairs.
PMID:20378837
Glutaminase 2, a novel p53 target gene regulating energy metabolism and antioxidant function.
PMID:20385133
p53 inhibits tumor cell invasion via the degradation of snail protein in hepatocellular carcinoma.
PMID:20421506
Mapping the physical and functional interactions between the tumor suppressors p53 and BRCA2.
PMID:20452352
A novel hPirh2 splicing variant without ubiquitin protein ligase activity interacts with p53 and is down-regulated in hepatocellular carcinoma.
PMID:20515689
MDM4 binds ligands via a mechanism in which disordered regions become structured.
PMID:20534433
Synaptonemal complex formation and meiotic checkpoint signaling are linked to the lateral element protein Red1.
PMID:20546595
High-throughput sequencing identifies STAT3 as the DNA-associated factor for p53-NF-kappaB-complex-dependent gene expression in human heart failure.
PMID:20562916
Dual-specificity phosphatase 26 is a novel p53 phosphatase and inhibits p53 tumor suppressor functions in human neuroblastoma.
PMID:20591429
S100 proteins interact with the N-terminal domain of MDM2.
PMID:20622899
PBK/TOPK interacts with the DBD domain of tumor suppressor p53 and modulates expression of transcriptional targets including p21.
PMID:20660729
Polybromo-associated BRG1-associated factor components BRD7 and BAF180 are critical regulators of p53 required for induction of replicative senescence.
PMID:20705607
Turning the RING domain protein MdmX into an active ubiquitin-protein ligase.
PMID:20708156
Phosphorylation by casein kinase I promotes the turnover of the Mdm2 oncoprotein via the SCF(beta-TRCP) ubiquitin ligase.
PMID:20713054
ARF-dependent regulation of ATM and p53 associated KZNF (Apak) protein activity in response to oncogenic stress.
PMID:20725088
Primate-specific RFPL1 gene controls cell-cycle progression through cyclin B1/Cdc2 degradation.
PMID:20810912
Hypoxia downregulates p53 but induces apoptosis and enhances expression of BAD in cultures of human syncytiotrophoblasts.
PMID:20864041
MAGE-RING protein complexes comprise a family of E3 ubiquitin ligases.
PMID:20959462
Aurora B interacts with NIR-p53, leading to p53 phosphorylation in its DNA-binding domain and subsequent functional suppression.
PMID:21057547
AXIN is an essential co-activator for the promyelocytic leukemia protein in p53 activation.
PMID:21078964
p53-mediated apoptosis requires inositol hexakisphosphate kinase-2.
PMID:21081126
Anti-apoptotic protein TCTP controls the stability of the tumor suppressor p53.
PMID:21130767
SAFB1 interacts with and suppresses the transcriptional activity of p53.
PMID:21132010
GNL3L depletion destabilizes MDM2 and induces p53-dependent G2/M arrest.
PMID:21170034
TSPYL5 suppresses p53 levels and function by physical interaction with USP7.
PMID:21170087
Ribosomal protein S27-like and S27 interplay with p53-MDM2 axis as a target, a substrate and a regulator.
PMID:21178074
Electron microscopy studies on the quaternary structure of p53 reveal different binding modes for p53 tetramers in complex with DNA.
PMID:21245319
Methyltransferase Set7/9 regulates p53 activity by interacting with Sirtuin 1 (SIRT1).
PMID:21317932
A new role of NUAK1: directly phosphorylating p53 and regulating cell proliferation.
PMID:21336310
p53 regulates biosynthesis through direct inactivation of glucose-6-phosphate dehydrogenase.
PMID:21390126
Inactivating mutations of acetyltransferase genes in B-cell lymphoma.
PMID:21397192
Interferon-inducible protein 16: insight into the interaction with tumor suppressor p53.
PMID:21423215
Camptothecin-induced downregulation of MLL5 contributes to the activation of tumor suppressor p53.
PMID:2144364
Transcriptional activation by wild-type but not transforming mutants of the p53 anti-oncogene.
PMID:21460856
ATM-mediated phosphorylation activates the tumor-suppressive function of B56Ξ³-PP2A.
PMID:21471221
Novel nucleolar pathway connecting intracellular energy status with p53 activation.
PMID:21513714
The ASPP proteins complex and cooperate with p300 to modulate the transcriptional activity of p53.
PMID:21522129
An induced fit mechanism regulates p53 DNA binding kinetics to confer sequence specificity.
PMID:21597459
E3 ubiquitin ligase Hades negatively regulates the exonuclear function of p53.
PMID:21625211
COP9 signalosome subunit 6 stabilizes COP1, which functions as an E3 ubiquitin ligase for 14-3-3Οƒ.
PMID:21653829
Protein interactome reveals converging molecular pathways among autism disorders.
PMID:21670263
Structural and functional characterization of an atypical activation domain in erythroid Kruppel-like factor (EKLF).
PMID:21726810
Caspase-2-mediated cleavage of Mdm2 creates a p53-induced positive feedback loop.
PMID:21741598
A Pin1/mutant p53 axis promotes aggressiveness in breast cancer.
PMID:21782458
Structural basis of substrate methylation and inhibition of SMYD2.
PMID:21821029
Aurora-A phosphorylates hnRNPK and disrupts its interaction with p53.
PMID:21831840
Wild-type p53 controls cell motility and invasion by dual regulation of MET expression.
PMID:21857681
A stress response pathway regulates DNA damage through Ξ²2-adrenoreceptors and Ξ²-arrestin-1.
PMID:21892170
Structural analysis of the interaction between Hsp90 and the tumor suppressor protein p53.
PMID:21900206
A directed protein interaction network for investigating intracellular signal transduction.
PMID:21952639
NIRF constitutes a nodal point in the cell cycle network and is a candidate tumor suppressor.
PMID:21988832
Toward an understanding of the protein interaction network of the human liver.
PMID:22056774
Bilateral inhibition of HAUSP deubiquitinase by a viral interferon regulatory factor protein.
PMID:22085928
Regulation of p53 stability and function by the deubiquitinating enzyme USP42.
PMID:22103682
Specific domains of nucleolin interact with Hdm2 and antagonize Hdm2-mediated p53 ubiquitination.
PMID:22124327
Positive regulation of p53 stability and activity by the deubiquitinating enzyme Otubain 1.
PMID:22265415
Mutant p53 disrupts mammary tissue architecture via the mevalonate pathway.
PMID:22340593
Aurora kinase-A inactivates DNA damage-induced apoptosis and spindle assembly checkpoint response functions of p73.
PMID:22451927
Role of the translationally controlled tumor protein in DNA damage sensing and repair.
PMID:22499945
Atg7 modulates p53 activity to regulate cell cycle and survival during metabolic stress.
PMID:22499991
Downregulation of endothelial microRNA-200b supports cutaneous wound angiogenesis by desilencing GATA binding protein 2 and vascular endothelial growth factor receptor 2.
PMID:22510990
AKT-dependent phosphorylation of Niban regulates nucleophosmin- and MDM2-mediated p53 stability and cell apoptosis.
PMID:22521434
Hepatocyte growth factor (HGF) and 1,25-dihydroxyvitamin D together stimulate human bone marrow-derived stem cells toward the osteogenic phenotype by HGF-induced up-regulation of VDR.
PMID:22522597
Nucleolar protein GLTSCR2 stabilizes p53 in response to ribosomal stresses.
PMID:22574148
p53 Activation following Rift Valley fever virus infection contributes to cell death and viral production.
PMID:22575647
An autoregulatory feedback loop between Mdm2 and SHP that fine tunes Mdm2 and SHP stability.
PMID:22578566
Oncosuppressive role of p53-induced miR-205 in triple negative breast cancer.
PMID:22653443
Mutant p53 interactome identifies nardilysin as a p53R273H-specific binding partner that promotes invasion.
PMID:22659184
Mutational analysis reveals a dual role of Mdm2 acidic domain in the regulation of p53 stability.
PMID:22723347
Differentiated embryo-chondrocyte expressed gene 1 regulates p53-dependent cell survival versus cell death through macrophage inhibitory cytokine-1.
PMID:22726440
p53 opens the mitochondrial permeability transition pore to trigger necrosis.
PMID:22783376
Induction of apoptosis by cytoplasmically localized wild-type p53 and the S121F mutant super p53.
PMID:22810585
Viral immune modulators perturb the human molecular network by common and unique strategies.
PMID:22810586
Interpreting cancer genomes using systematic host network perturbations by tumour virus proteins.
PMID:22819825
TRIAD1 inhibits MDM2-mediated p53 ubiquitination and degradation.
PMID:22869143
BMK1 is involved in the regulation of p53 through disrupting the PML-MDM2 interaction.
PMID:22945289
ZNF385B is characteristically expressed in germinal center B cells and involved in B-cell apoptosis.
PMID:22972749
Domain-domain interactions in full-length p53 and a specific DNA complex probed by methyl NMR spectroscopy.
PMID:22975381
A "twist box" code of p53 inactivation: twist box: p53 interaction promotes p53 degradation.
PMID:23010591
Structural features of human histone acetyltransferase p300 and its complex with p53.
PMID:23027130
TrkC signaling is activated in adenoid cystic carcinoma and requires NT-3 to stimulate invasive behavior.
PMID:23063560
HMGB1-facilitated p53 DNA binding occurs via HMG-Box/p53 transactivation domain interaction, regulated by the acidic tail.
PMID:23092970
SUMOylation of hnRNP-K is required for p53-mediated cell-cycle arrest in response to DNA damage.
PMID:23320542
Evidence for self-association of the alternative sigma factor Οƒ54.
PMID:23329847
Regulation of the cyclin-dependent kinase inhibitor 1A gene (CDKN1A) by the repressor BOZF1 through inhibition of p53 acetylation and transcription factor Sp1 binding.
PMID:23431171
MOZ increases p53 acetylation and premature senescence through its complex formation with PML.
PMID:23576507
Rotavirus-encoded nonstructural protein 1 modulates cellular apoptotic machinery by targeting tumor suppressor protein p53.
PMID:23623661
Restoring p53 function in human melanoma cells by inhibiting MDM2 and cyclin B1/CDK1-phosphorylated nuclear iASPP.
PMID:23629966
Deacetylation of p53 induces autophagy by suppressing Bmf expression.
PMID:23734815
Fludarabine treatment favors the retention of miR-485-3p by prostate cancer cells: implications for survival.
PMID:23752197
S100A4 interacts with p53 in the nucleus and promotes p53 degradation.
PMID:23776060
Identification of a second Nutlin-3 responsive interaction site in the N-terminal domain of MDM2 using hydrogen/deuterium exchange mass spectrometry.
PMID:23870121
SET1 and p300 act synergistically, through coupled histone modifications, in transcriptional activation by p53.
PMID:24051492
p53 regulates Period2 expression and the circadian clock.
PMID:24101517
Mitochondrial disulfide relay mediates translocation of p53 and partitions its subcellular activity.
PMID:24207125
Transient protein states in designing inhibitors of the MDM2-p53 interaction.
PMID:24219989
Acetylation of p53 stimulates miRNA processing and determines cell survival following genotoxic stress.
PMID:24289924
Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response.
PMID:24356969
Rbm24, an RNA-binding protein and a target of p53, regulates p21 expression via mRNA stability.
PMID:24380853
The aberrant expression and localization of prohibitin during apoptosis of human cholangiocarcinoma Mz-ChA-1 cells.
PMID:24449765
Activation of p53 transcriptional activity by SMRT: a histone deacetylase 3-independent function of a transcriptional corepressor.
PMID:24492002
VRK1 interacts with p53 forming a basal complex that is activated by UV-induced DNA damage.
PMID:24625977
Ubiquitin-like (UBX)-domain-containing protein, UBXN2A, promotes cell death by interfering with the p53-Mortalin interactions in colon cancer cells.
PMID:24652652
DRAGO (KIAA0247), a new DNA damage-responsive, p53-inducible gene that cooperates with p53 as oncosuppressor. [Corrected].
PMID:24667498
JMJD6 promotes colon carcinogenesis through negative regulation of p53 by hydroxylation.
PMID:24722188
Protein interaction network of alternatively spliced isoforms from brain links genetic risk factors for autism.
PMID:24814347
The DNA-binding domain mediates both nuclear and cytosolic functions of p53.
PMID:25168243
Cofactor Strap regulates oxidative phosphorylation and mitochondrial p53 activity through ATP synthase.
PMID:25241761
Using an in situ proximity ligation assay to systematically profile endogenous protein-protein interactions in a pathway network.
PMID:25314079
Acetylation of snail modulates the cytokinome of cancer cells to enhance the recruitment of macrophages.
PMID:25384516
Rad54B serves as a scaffold in the DNA damage response that limits checkpoint strength.
PMID:25402006
In silico prediction of physical protein interactions and characterization of interactome orphans.
PMID:25417702
Functional interplay between MDM2, p63/p73 and mutant p53.
PMID:25422469
Disruption of FAT10-MAD2 binding inhibits tumor progression.
PMID:25502805
A massively parallel pipeline to clone DNA variants and examine molecular phenotypes of human disease mutations.
PMID:25579814
Structural plasticity of methyllysine recognition by the tandem tudor domain of 53BP1.
PMID:25591766
RNF125 is a ubiquitin-protein ligase that promotes p53 degradation.
PMID:25609649
Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes.
PMID:25651062
An acetyl-methyl switch drives a conformational change in p53.
PMID:25670079
14-3-3ΞΆ turns TGF-Ξ²'s function from tumor suppressor to metastasis promoter in breast cancer by contextual changes of Smad partners from p53 to Gli2.
PMID:25837623
Regulation of protein quality control by UBE4B and LSD1 through p53-mediated transcription.
PMID:25857266
Pontin, a new mutant p53-binding protein, promotes gain-of-function of mutant p53.
PMID:26100857
A metabolic stress-inducible miR-34a-HNF4Ξ± pathway regulates lipid and lipoprotein metabolism.
PMID:26302407
Combined CSL and p53 downregulation promotes cancer-associated fibroblast activation.
PMID:26331536
Gain-of-function p53 mutants co-opt chromatin pathways to drive cancer growth.
PMID:26334721
Jmjd5 functions as a regulator of p53 signaling during mouse embryogenesis.
PMID:26634371
Structural studies of UBXN2A and mortalin interaction and the putative role of silenced UBXN2A in preventing response to chemotherapy.
PMID:26789255
Structure of the E6/E6AP/p53 complex required for HPV-mediated degradation of p53.
PMID:27031958
Nupr1/Chop signal axis is involved in mitochondrion-related endothelial cell apoptosis induced by methamphetamine.
PMID:27107012
Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
PMID:27323408
LACE1 interacts with p53 and mediates its mitochondrial translocation and apoptosis.
PMID:27519799
p53 down-regulates SARS coronavirus replication and is targeted by the SARS-unique domain and PLpro via E3 ubiquitin ligase RCHY1.
PMID:27605672
Mitogen-Activated Protein Kinase Kinase 2, a Novel E2-Interacting Protein, Promotes the Growth of Classical Swine Fever Virus via Attenuation of the JAK-STAT Signaling Pathway.
PMID:28842590
DDX3 localizes to the centrosome and prevents multipolar mitosis by epigenetically and translationally modulating p53 expression.
PMID:29187402
The E3 Ligase RING1 Targets p53 for Degradation and Promotes Cancer Cell Proliferation and Survival.
PMID:29340707
VRK1 and AURKB form a complex that cross inhibit their kinase activity and the phosphorylation of histone H3 in the progression of mitosis.
PMID:29474172
Haploinsufficiency of Trp53 dramatically extends the lifespan of Sirt6-deficient mice.
PMID:29628311
A Family of Vertebrate-Specific Polycombs Encoded by the LCOR/LCORL Genes Balance PRC2 Subtype Activities.
PMID:29681526
A Designed Peptide Targets Two Types of Modifications of p53 with Anti-cancer Activity.
PMID:29997244
LuTHy: a double-readout bioluminescence-based two-hybrid technology for quantitative mapping of protein-protein interactions in mammalian cells.
PMID:30146126
De Novo Mutations Activating Germline TP53 in an Inherited Bone-Marrow-Failure Syndrome.
PMID:31467278
Maximizing binary interactome mapping with a minimal number of assays.
PMID:31511497
miR-146a attenuates apoptosis and modulates autophagy by targeting TAF9b/P53 pathway in doxorubicin-induced cardiotoxicity.
PMID:31515488
Extensive disruption of protein interactions by genetic variants across the allele frequency spectrum in human populations.
PMID:31837246
High-throughput competitive fluorescence polarization assay reveals functional redundancy in the S100 protein family.
PMID:31953488
Liquid-like droplet formation by tumor suppressor p53 induced by multivalent electrostatic interactions between two disordered domains.
PMID:32606738
P55PIK Regulates P53-Dependent Apoptosis in Cancer Cells by Interacting with P53 DNA-Specific Domain.
PMID:32814053
Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.
PMID:33591310
DAZAP2 acts as specifier of the p53 response to DNA damage.
PMID:33961781
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
PMID:34316702
Elucidation of the BMI1 interactome identifies novel regulatory roles in glioblastoma.
PMID:34381247
The tumor suppressor folliculin inhibits lactate dehydrogenase A and regulates the Warburg effect.
PMID:34404770
ZNF768 links oncogenic RAS to cellular senescence.
PMID:34591612
A protein interaction landscape of breast cancer.
PMID:34591642
A protein network map of head and neck cancer reveals PIK3CA mutant drug sensitivity.
PMID:35044719
Proteome-scale mapping of binding sites in the unstructured regions of the human proteome.
PMID:35122041
Aldolase B suppresses hepatocellular carcinogenesis by inhibiting G6PD and pentose phosphate pathways.
PMID:35140242
Human transcription factor protein interaction networks.
PMID:35271311
OpenCell: Endogenous tagging for the cartography of human cellular organization.
PMID:35512704
Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.
PMID:35618207
Ser392 phosphorylation modulated a switch between p53 and transcriptional condensates.
PMID:36108750
Phase separation of p53 induced by its unstructured basic region and prevented by oncogenic mutations in tetramerization domain.
PMID:36634798
Phosphorylation and specific DNA improved the incorporation ability of p53 into functional condensates.
PMID:36931259
A central chaperone-like role for 14-3-3 proteins in human cells.
PMID:38653238
Alanyl-tRNA synthetase, AARS1, is a lactate sensor and lactyltransferase that lactylates p53 and contributes to tumorigenesis.
PMID:39009827
Proteome-scale characterisation of motif-based interactome rewiring by disease mutations.
PMID:7559631
Transactivation ability of p53 transcriptional activation domain is directly related to the binding affinity to TATA-binding protein.
PMID:7587074
Targets for transcriptional activation by wild-type p53: endogenous retroviral LTR, immunoglobulin-like promoter, and an internal promoter of the mdm2 gene.
PMID:7663514
p53 modulation of TFIIH-associated nucleotide excision repair activity.
PMID:7720704
Direct involvement of p53 in programmed cell death of oligodendrocytes.
PMID:7799929
Two domains of p53 interact with the TATA-binding protein, and the adenovirus 13S E1A protein disrupts the association, relieving p53-mediated transcriptional repression.
PMID:7824276
Modulation by copper of p53 conformation and sequence-specific DNA binding: role for Cu(II)/Cu(I) redox mechanism.
PMID:7958916
DNA damage triggers a prolonged p53-dependent G1 arrest and long-term induction of Cip1 in normal human fibroblasts.
PMID:8183576
Human p53 directs DNA strand reassociation and is photolabelled by 8-azido ATP.
PMID:8207801
Inhibition of p53 DNA binding by human papillomavirus E6 proteins.
PMID:8344494
Identification of mutations in p53 that affect its binding to SV40 large T antigen by using the yeast two-hybrid system.
PMID:8675009
The XPB and XPD DNA helicases are components of the p53-mediated apoptosis pathway.
PMID:8875926
Structure of the p53 tumor suppressor bound to the ankyrin and SH3 domains of 53BP2.
PMID:8875929
Structure of the MDM2 oncoprotein bound to the p53 tumor suppressor transactivation domain.
PMID:8986812
Identification of a novel p53 functional domain that is necessary for efficient growth suppression.
PMID:9050995
Identification of p53 unbound to T-antigen in human cells transformed by simian virus 40 T-antigen.
PMID:9054499
Oncogenic ras provokes premature cell senescence associated with accumulation of p53 and p16INK4a.
PMID:9188558
Nuclear localization of the NS3 protein of hepatitis C virus and factors affecting the localization.
PMID:9194564
Synergistic activation of transcription by CBP and p53.
PMID:9194565
Binding and modulation of p53 by p300/CBP coactivators.
PMID:9271120
Repression of p53-mediated transcription by MDM2: a dual mechanism.
PMID:9380510
Interaction of p53 with the human Rad51 protein.
PMID:9472015
High mobility group protein-1 (HMG-1) is a unique activator of p53.
PMID:9529249
ARF promotes MDM2 degradation and stabilizes p53: ARF-INK4a locus deletion impairs both the Rb and p53 tumor suppression pathways.
PMID:9733515
Activation of the ATM kinase by ionizing radiation and phosphorylation of p53.
PMID:9807817
An Arabidopsis immunophilin, AtFKBP12, binds to AtFIP37 (FKBP interacting protein) in an interaction that is disrupted by FK506.
PMID:9827557
Complex formation of the nonstructural protein 3 of hepatitis C virus with the p53 tumor suppressor.
Reactome:R-HSA-264435
Dissociation of the COP1-p53 complex
Reactome:R-HSA-2997706
MDM2 SUMOylates TP53 with SUMO2,3
Reactome:R-HSA-3215152
TP53 in complex with EP300, PRMT1 and CARM1 binds the GADD45A promoter
Reactome:R-HSA-3215251
TP53INP1 and HIPK2 bind TP53
Reactome:R-HSA-3215310
USP7 deubiquitinates TP53 and counteracts MDM2
Reactome:R-HSA-3221982
BANP binds TP53
Reactome:R-HSA-3222006
STK11 (LKB1) phosphorylates NUAK1
Reactome:R-HSA-3222020
NUAK1 phosphorylates TP53
Reactome:R-HSA-3222093
BRD7 binds TP53 and EP300
Reactome:R-HSA-3222116
L3MBTL1 binds TP53 monomethylated on K382
Reactome:R-HSA-3222128
NOC2L binds TP53
Reactome:R-HSA-3222237
SMYD2 methylates TP53
Reactome:R-HSA-3222259
PHF20 binds TP53 dimethylated on K370 and K382
Reactome:R-HSA-3239014
MAPKAPK5 phosphorylates TP53
Reactome:R-HSA-3700981
TP53 family members bind the BAX promoter
Reactome:R-HSA-3700997
TP53 binds the MDM2 gene
Reactome:R-HSA-3786258
TP53 binds the CDKN1A promoter
Reactome:R-HSA-4331331
TP53 binds the PMAIP1 (NOXA) promoter
Reactome:R-HSA-4331340
TP53 binds the BBC3 (PUMA) promoter
Reactome:R-HSA-4395236
TP53 binds promoters of MIR34 genes
Reactome:R-HSA-4647593
EP400 binds CDKN1A promoter
Reactome:R-HSA-5628871
BRD7 promotes EP300-mediated acetylation of TP53
Reactome:R-HSA-5628899
TP53 binds the TIGAR gene
Reactome:R-HSA-5629187
TP53 binds regulatory elements of SESN1,2,3 genes
Reactome:R-HSA-5632759
TP53 binds the SCO2 gene
Reactome:R-HSA-5632887
TP53 binds the RRM2B gene
Reactome:R-HSA-5632914
TP53 binds the GLS2 promoter
Reactome:R-HSA-5632939
TP53 binds the PTEN promoter
Reactome:R-HSA-5633295
TP53 binds the NDRG1 gene promoter
Reactome:R-HSA-5633414
TP53 binds TNFRSF10A,TNFRSF10B,TNFRSF10C,TNFRSF10D genes
Reactome:R-HSA-5633460
MDM2 binds TP53
Reactome:R-HSA-5689950
USP7 deubiquitinates TP53,MDM2,MDM4,FOXO4, PTEN
Reactome:R-HSA-5689973
USP10,USP24,USP42 deubiquitinate TP53
Reactome:R-HSA-5690843
OTUB1, (OTUB2) binds RNF128, TRAF3, TRAF6, RHOA
Reactome:R-HSA-5693609
ATM phosphorylates TP53 at S15
Reactome:R-HSA-6789960
Expression of NDN,TP53
Reactome:R-HSA-6791285
TP53 binds the BID gene promoter
Reactome:R-HSA-6791302
TP53 binds the AIFM2 gene promoter
Reactome:R-HSA-6791327
TP53 binds the BCL2L14 gene
Reactome:R-HSA-6791349
TP53 binds the APAF1 gene promoter
Reactome:R-HSA-6791387
TP53 binds the ARID3A gene
Reactome:R-HSA-6791409
TP53 binds the CCNG1 gene
Reactome:R-HSA-6793685
Ubiquitinated TP53 translocates to the cytosol
Reactome:R-HSA-6796649
TP53 binds the CCNK gene
Reactome:R-HSA-6797766
TP53 binds the BIRC5 gene
Reactome:R-HSA-6797993
TP53 and CREBBP bind BNIP3L gene
Reactome:R-HSA-6798020
TP53 binds the BTG2 gene
Reactome:R-HSA-6798082
TP53 family members bind the CASP1 gene
Reactome:R-HSA-6798129
TP53 binds the CASP6 gene
Reactome:R-HSA-6798138
TP53 binds the CASP10 gene
Reactome:R-HSA-6798282
TP53 and E2F4 bind the CDC25C gene
Reactome:R-HSA-6798304
TP53 binds the E2F7 gene
Reactome:R-HSA-6798374
DYRK2 phosphorylates TP53
Reactome:R-HSA-6798615
TP53 binds the TP53AIP1 gene
Reactome:R-HSA-6799246
CHEK1 phosphorylates TP53
Reactome:R-HSA-6799332
ATR phosphorylates TP53
Reactome:R-HSA-6799409
HIPK2 phosphorylates TP53
Reactome:R-HSA-6799418
TP53 binds the TP53INP1 gene
Reactome:R-HSA-6799431
TP53 binds HIPK1
Reactome:R-HSA-6799462
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file:human/TP53/TP53-deep-research-falcon.md
Deep research report on TP53

Suggested Questions for Experts

Q: How does p53 integrate diverse stress signals to determine cell fate decisions between survival, senescence, and apoptosis?

Q: What determines the selectivity of p53 for different target gene promoters and how is this modulated by post-translational modifications?

Q: How do p53 isoforms and mutant forms interact to modulate tumor suppressor function in heterozygous cancer cells?

Suggested Experiments

Experiment: Single-cell time-lapse imaging to track p53 dynamics and correlate oscillation patterns with specific cell fate outcomes

Experiment: ChIP-seq coupled with PRO-seq to map p53 binding and transcriptional outcomes under different stress conditions

Experiment: Proximity labeling proteomics to identify context-specific p53 interactors that determine target gene selectivity

Tags

ferroptosis lbnl-favorites

πŸ“š Additional Documentation

Deep Research

(TP53-deep-research.md)

TP53 (Tumor Protein p53) - Comprehensive Deep Research

Gene Overview

TP53 encodes the tumor suppressor p53, widely recognized as the "guardian of the genome" due to its central role in maintaining genomic integrity and preventing malignant transformation. Located on chromosome 17p13.1, TP53 is the most frequently altered gene in human cancers, with mutations found in over 50% of all human malignancies [PMC11641486 "p53 plays a multifaceted and complex role in the DNA damage response and cancer progression and is known as the 'guardian of the gene'"]. The protein product consists of 393 amino acids and functions as a sequence-specific transcription factor that coordinates cellular responses to diverse stress signals.

Core Molecular Functions

DNA-Binding Transcription Factor Activity

TP53 is fundamentally a transcription factor that binds sequence-specifically to p53 response elements (p53REs) in target gene promoters and regulatory regions [Nature 2023 "The p53 protein is a transcription factor that can activate the expression of multiple target genes and plays critical roles in regulating cell cycle, apoptosis, and genomic stability"]. This DNA-binding activity is mediated by its central DNA-binding domain (DBD) and is essential for virtually all p53 functions.

Protein-Protein Interactions

p53 functions through extensive protein-protein interactions, including:
- Homodimerization and tetramerization through its oligomerization domain
- Interactions with transcriptional co-activators (CBP/p300, TFIID)
- Binding to negative regulators (MDM2, MDM4)
- Complex formation with DNA repair proteins and cell cycle regulators

Transcriptional Regulation

p53 acts as both a transcriptional activator and repressor:
- Activation: Upregulates genes involved in cell cycle arrest (p21/CDKN1A), apoptosis (PUMA, BAX, NOXA), and DNA repair
- Repression: Downregulates anti-apoptotic genes (BCL2), cell cycle progression genes, and metabolic enzymes [Nature 2023 "p53 is not a direct repressor of genes but can indirectly repress genes through activation of the p21-DREAM/RB pathway"]

Key Biological Processes

DNA Damage Response

p53 serves as a central node in the DNA damage response network [PMC11641486 "When DNA damage occurs, p53 is activated through a series of post-translational modifications, which stabilize the protein and enhance its function as a transcription factor"]. It integrates signals from upstream kinases (ATM, ATR, DNA-PK) and coordinates appropriate cellular responses including:
- Cell cycle checkpoint activation
- DNA repair pathway stimulation
- Apoptosis induction when damage is irreparable

Cell Cycle Control

p53 enforces cell cycle checkpoints primarily through transcriptional activation of p21 (CDKN1A), which inhibits cyclin-dependent kinases and prevents S-phase entry with unrepaired DNA damage [Multiple sources "Cell cycle arrest is mediated by transcriptional upregulation of p21, a cyclin-dependent kinase inhibitor that binds and inhibits CDK2"].

Apoptosis and Cell Death

p53 triggers apoptosis through both transcription-dependent and transcription-independent mechanisms:
- Transcriptional: Activation of pro-apoptotic genes (PUMA, BAX, NOXA, FAS)
- Non-transcriptional: Direct mitochondrial translocation and interaction with BCL-2 family proteins

Cellular Senescence

p53 induces senescence as an alternative to apoptosis, particularly in stressed but viable cells, serving as a barrier to tumorigenesis.

Metabolic Regulation

Recent research has revealed p53's extensive role in cellular metabolism [Nature 2023 "Accumulating evidence has shown that p53 also regulates cell metabolism, ferroptosis, tumor microenvironment, autophagy and so on"]:
- Wild-type p53: Inhibits glycolysis, promotes oxidative phosphorylation and mitochondrial respiration
- Mutant p53: Enhances aerobic glycolysis (Warburg effect) in cancer cells
- Specific targets: TIGAR (inhibits glycolysis), SCO2 (promotes oxidative phosphorylation), GLS2 (glutaminolysis)

Ferroptosis Regulation

p53 has a dual role in ferroptosis (iron-dependent programmed cell death) [PMC10875350 "p53 regulates ferroptosis pathways within tumor cells and is closely related to tumorigenesis"]:
- Pro-ferroptotic: Represses SLC7A11, activates ALOX12, promotes lipid peroxidation
- Anti-ferroptotic: Induces p21 to maintain GSH levels, inhibits DPP4 activity

Protein Structure and Functional Domains

Transactivation Domains (TADs)

  • TAD1 (residues 1-42): Primary transactivation domain
  • TAD2 (residues 43-63): Secondary transactivation domain
  • Both domains interact with transcriptional machinery and co-activators

Proline-Rich Domain (PRD)

  • Location: Residues ~64-92
  • Function: Protein-protein interactions, regulation of transcriptional activity

DNA-Binding Domain (DBD)

  • Location: Residues 102-292
  • Structure: Ξ²-sandwich core with DNA-binding loops
  • Critical residues: R175, R248, R273 (frequent cancer mutation sites)
  • Function: Sequence-specific DNA binding to p53 response elements

Nuclear Localization Signals (NLS)

  • Location: Residues 305-322 and 369-375
  • Function: Nuclear import and localization

Oligomerization Domain (OD)

  • Location: Residues 325-356
  • Structure: Ξ²-strand and Ξ±-helix connected by turn at Gly334
  • Function: Dimerization and tetramerization essential for DNA binding

C-Terminal Domain (CTD)

  • Location: Residues 357-393
  • Function: Regulatory domain, contains multiple lysine residues for post-translational modifications

Post-Translational Modifications

p53 can be modified at over 60 of its 393 residues, with major modifications including:

Phosphorylation

Key phosphorylation sites and their functions:
- Ser15, Thr18, Ser20: N-terminal phosphorylation by ATM/ATR, disrupts MDM2 binding
- Ser46: Promotes apoptosis over cell cycle arrest
- Ser392: C-terminal phosphorylation, enhances DNA binding

Acetylation

Critical for p53 activation and stability:
- Lys120, Lys164: Central domain acetylation
- Lys370, Lys372, Lys373, Lys381, Lys382, Lys386: C-terminal acetylation
- Function: Stabilizes p53, enhances DNA binding, recruits co-activators [PMC9149126 "The significance of p53 acetylation is 3-fold: (i) it promotes p53 stabilization by excluding ubiquitination on the same site, (ii) it inhibits the formation of HDM2/HDMX repressive complexes"]

Ubiquitination

Primary mechanism for p53 degradation:
- E3 ligases: MDM2, MDM4, COP1, TRIM24
- Sites: Multiple C-terminal lysines
- Function: Proteasomal degradation, nuclear export

Methylation

Less understood but increasingly recognized:
- Sites: Lys370, Lys372, Lys373, Lys382
- Function: Fine-tuning of transcriptional activity

Target Genes and Pathways

Cell Cycle Arrest

  • p21 (CDKN1A): Primary effector of G1/S checkpoint
  • 14-3-3Οƒ (SFN): G2/M checkpoint control
  • GADD45A: DNA repair and cell cycle control

Apoptosis

  • PUMA (BBC3): Pro-apoptotic BH3-only protein
  • BAX: Pro-apoptotic BCL-2 family member
  • NOXA (PMAIP1): Pro-apoptotic BH3-only protein
  • FAS: Death receptor pathway activation
  • APAF1: Apoptosome formation

DNA Repair

  • XPC: Nucleotide excision repair
  • DDB2: DNA damage-binding protein
  • MSH2: Mismatch repair

Negative Feedback

  • MDM2: Primary negative regulator, creates auto-regulatory loop
  • MDM4: Co-regulator of p53 stability

Metabolism

  • TIGAR: Inhibits glycolysis, reduces ROS
  • SCO2: Promotes oxidative phosphorylation
  • GLS2: Glutaminolysis regulation

Disease Associations

Li-Fraumeni Syndrome (LFS)

Germline TP53 mutations cause Li-Fraumeni syndrome, characterized by:
- Early-onset multiple primary cancers
- Autosomal dominant inheritance pattern
- Common hotspot mutations: R175H, R248Q, R273H, G245S, R282W
- R248Q specific: Particularly aggressive phenotype with accelerated tumor onset [Recent 2024 studies "LFS patients carrying the R248Q mutation exhibit markedly accelerated tumor onset (10.5 years earlier)"]

Cancer Mutations

  • Frequency: >50% of human cancers harbor TP53 mutations
  • Type: Predominantly missense mutations in DNA-binding domain
  • Hotspots: Codons 175, 245, 248, 249, 273, 282
  • Functional impact: Loss of tumor suppressor function, potential gain-of-function activities

Loss of Heterozygosity (LOH)

  • Frequency: 86% of LFS tumors show TP53 LOH
  • Exception: R248Q carriers less frequently show LOH, suggesting dominant-negative effects

Therapeutic Targeting

MDM2 Inhibitors

Several MDM2 inhibitors are in clinical development to reactivate wild-type p53:
- HDM201 (siremadlin): Selective MDM2 inhibitor
- ALRN-6924: Dual MDM2/MDM4 inhibitor
- NVP-CGM097: Dihydroisoquinolinone derivative
- Clinical challenges: Dose-limiting toxicities, patient selection [Recent review 2024 "Nine MDM2 inhibitors with different structural types have entered clinical trials, though no MDM2 inhibitor has been approved for clinical application yet"]

p53 Reactivation

  • APR-246 (Eprenetapopt): Small molecule that restores wild-type function to mutant p53
  • Clinical results: 71% overall response rate in TP53-mutant myeloid neoplasms [2024 data "Of 55 patients with at least one TP53 mutation treated, the overall response rate was 71%, with 44% achieving CR"]

Synthetic Lethality

  • ATM/ATR inhibitors: Target DNA repair defects in p53-mutant cells
  • PARP inhibitors: Exploit homologous recombination defects
  • Cell cycle checkpoint inhibitors: Target G2/M checkpoint dependence

Immunotherapy Approaches

Recent research has uncovered p53's immunomodulatory functions [PMC11393945 "Recent studies have uncovered important immunomodulatory functions of p53, but these functions are still underappreciated compared to its other well-known roles"], opening new avenues for combination immunotherapy.

Key Experimental Findings and Citations

Foundational Discoveries

  • Discovery of p53 as cellular tumor antigen PMID:4006916
  • Identification as tumor suppressor [Multiple early studies established tumor suppressor function]
  • DNA-binding transcription factor activity PMID:15710329

Recent Paradigm Shifts (2023-2024)

  • Metabolic regulation beyond cell cycle/apoptosis [Nature 2023 "although early studies established p53-mediated cell-cycle arrest, apoptosis, and senescence as the classic barriers in cancer development, a growing number of new functions of p53 have been discovered"]
  • Ferroptosis regulation [PMC10875350 "P53 together with ferroptosis: a promising strategy leaving cancer cells without escape"]
  • Immunomodulatory functions [PMC11393945 "TP53 Mutation-Mediated Immune Evasion in Cancer: Mechanisms and Therapeutic Implications"]

Structural and Mechanistic Insights

  • Oligomerization requirements for DNA binding [Multiple studies "High-affinity DNA binding requires the formation of tetramers which enables dominant negative effects of cancer mutations"]
  • Post-translational modification crosstalk [Academic sources "Crosstalk between p53 modifications includes homogeneous modification crosstalk vs. heterogeneous modification crosstalk"]
  • Isoform functionality [eLife 2024 "With the exception of the Ξ”40p53Ξ± isoform, none of the other variants can bind to DNA with high affinity"]

Commonly Over-Annotated Aspects

Non-Specific Protein Binding

While p53 does interact with numerous proteins, annotations for generic "protein binding" without functional context should be avoided in favor of specific functional interactions.

Peripheral Developmental Processes

Although p53 plays roles in development and embryogenesis, these are often secondary to its core tumor suppressor functions and may represent over-annotation in certain contexts.

Context-Dependent Functions

Some metabolic and stress response functions may be context-dependent and should not be considered universal p53 functions across all cell types and conditions.

Summary

TP53 represents one of the most extensively studied genes in cancer biology, with its protein product serving multiple critical roles beyond the traditional "guardian of the genome" function. Recent research has expanded our understanding to include metabolic regulation, ferroptosis control, and immunomodulation. The complexity of p53 regulation through extensive post-translational modifications and its context-dependent functions continue to reveal new therapeutic opportunities, particularly in the era of precision medicine and combination therapies. The high frequency of TP53 mutations in human cancers and the aggressive nature of certain hotspot mutations like R248Q underscore the continued importance of developing effective p53-targeted therapeutics.

Deep Research Falcon

(TP53-deep-research-falcon.md)

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Question

Gene Research for Functional Annotation

⚠️ CRITICAL: Gene/Protein Identification Context

BEFORE YOU BEGIN RESEARCH: You MUST verify you are researching the CORRECT gene/protein. Gene symbols can be ambiguous, especially for less well-characterized genes from non-model organisms.

Target Gene/Protein Identity (from UniProt):

  • UniProt Accession: P04637
  • Protein Description: RecName: Full=Cellular tumor antigen p53; AltName: Full=Antigen NY-CO-13; AltName: Full=Phosphoprotein p53; AltName: Full=Tumor suppressor p53;
  • Gene Information: Name=TP53; Synonyms=P53;
  • Organism (full): Homo sapiens (Human).
  • Protein Family: Belongs to the p53 family. .
  • Key Domains: p53-like_TF_DNA-bd_sf. (IPR008967); p53/RUNT-type_TF_DNA-bd_sf. (IPR012346); p53_DNA-bd. (IPR011615); p53_TAD2. (IPR040926); p53_tetramer_sf. (IPR036674)

MANDATORY VERIFICATION STEPS:

  1. Check if the gene symbol "TP53" matches the protein description above
  2. Verify the organism is correct: Homo sapiens (Human).
  3. Check if protein family/domains align with what you find in literature
  4. If you find literature for a DIFFERENT gene with the same or similar symbol, STOP

If Gene Symbol is Ambiguous or You Cannot Find Relevant Literature:

DO NOT PROCEED WITH RESEARCH ON A DIFFERENT GENE. Instead:
- State clearly: "The gene symbol 'TP53' is ambiguous or literature is limited for this specific protein"
- Explain what you found (e.g., "Found extensive literature on a different gene with the same symbol in a different organism")
- Describe the protein based ONLY on the UniProt information provided above
- Suggest that the protein function can be inferred from domain/family information

Research Target:

Please provide a comprehensive research report on the gene TP53 (gene ID: TP53, UniProt: P04637) in human.

The research report should be a detailed narrative explaining the function, biological processes, and localization of the gene product. Citations should be given for all claims.

You should prioritize authoritative reviews and primary scientific literature when conducting research. You can supplement
this with annotations you find in gene/protein databases, but these can be outdated or inaccurate.

We are specifically interested in the primary function of the gene - for enzymes, what reaction is catalyzed, and what is the substrate specificity? For transporters, what is the substrate? For structural proteins or adapters, what is the broader structural role? For signaling molecules, what is the role in the pathway.

We are interested in where in or outside the cell the gene product carries out its function.

We are also interested in the signaling or biochemical pathways in which the gene functions. We are less interested in broad pleiotropic effects, except where these elucidate the precise role.

Include evidence where possible. We are interested in both experimental evidence as well as inference from structure, evolution, or bioinformatic analysis. Precise studies should be prioritized over high-throughput, where available.

Output

Question: You are an expert researcher providing comprehensive, well-cited information.

Provide detailed information focusing on:
1. Key concepts and definitions with current understanding
2. Recent developments and latest research (prioritize 2023-2024 sources)
3. Current applications and real-world implementations
4. Expert opinions and analysis from authoritative sources
5. Relevant statistics and data from recent studies

Format as a comprehensive research report with proper citations. Include URLs and publication dates where available.
Always prioritize recent, authoritative sources and provide specific citations for all major claims.

Gene Research for Functional Annotation

⚠️ CRITICAL: Gene/Protein Identification Context

BEFORE YOU BEGIN RESEARCH: You MUST verify you are researching the CORRECT gene/protein. Gene symbols can be ambiguous, especially for less well-characterized genes from non-model organisms.

Target Gene/Protein Identity (from UniProt):

  • UniProt Accession: P04637
  • Protein Description: RecName: Full=Cellular tumor antigen p53; AltName: Full=Antigen NY-CO-13; AltName: Full=Phosphoprotein p53; AltName: Full=Tumor suppressor p53;
  • Gene Information: Name=TP53; Synonyms=P53;
  • Organism (full): Homo sapiens (Human).
  • Protein Family: Belongs to the p53 family. .
  • Key Domains: p53-like_TF_DNA-bd_sf. (IPR008967); p53/RUNT-type_TF_DNA-bd_sf. (IPR012346); p53_DNA-bd. (IPR011615); p53_TAD2. (IPR040926); p53_tetramer_sf. (IPR036674)

MANDATORY VERIFICATION STEPS:

  1. Check if the gene symbol "TP53" matches the protein description above
  2. Verify the organism is correct: Homo sapiens (Human).
  3. Check if protein family/domains align with what you find in literature
  4. If you find literature for a DIFFERENT gene with the same or similar symbol, STOP

If Gene Symbol is Ambiguous or You Cannot Find Relevant Literature:

DO NOT PROCEED WITH RESEARCH ON A DIFFERENT GENE. Instead:
- State clearly: "The gene symbol 'TP53' is ambiguous or literature is limited for this specific protein"
- Explain what you found (e.g., "Found extensive literature on a different gene with the same symbol in a different organism")
- Describe the protein based ONLY on the UniProt information provided above
- Suggest that the protein function can be inferred from domain/family information

Research Target:

Please provide a comprehensive research report on the gene TP53 (gene ID: TP53, UniProt: P04637) in human.

The research report should be a detailed narrative explaining the function, biological processes, and localization of the gene product. Citations should be given for all claims.

You should prioritize authoritative reviews and primary scientific literature when conducting research. You can supplement
this with annotations you find in gene/protein databases, but these can be outdated or inaccurate.

We are specifically interested in the primary function of the gene - for enzymes, what reaction is catalyzed, and what is the substrate specificity? For transporters, what is the substrate? For structural proteins or adapters, what is the broader structural role? For signaling molecules, what is the role in the pathway.

We are interested in where in or outside the cell the gene product carries out its function.

We are also interested in the signaling or biochemical pathways in which the gene functions. We are less interested in broad pleiotropic effects, except where these elucidate the precise role.

Include evidence where possible. We are interested in both experimental evidence as well as inference from structure, evolution, or bioinformatic analysis. Precise studies should be prioritized over high-throughput, where available.

Plan (high-level)
- Verified the exact identity of the human target (TP53; UniProt P04637) and its domain architecture and quaternary structure, ensuring no symbol ambiguity. Built a concise verification artifact for reuse. (See embedded artifact.)
- Prioritized 2023–2024 authoritative sources (reviews and recent primary reports) to synthesize: (1) current concepts/definitions; (2) recent developments; (3) current applications/implementations; (4) expert opinions; (5) recent statistics/data.
- Focused on precise functional roles, subcellular localization, and pathway-level mechanisms (regulation by MDM2/MDM4; DNA damage response; apoptosis; ferroptosis; chromatin-level control), and on translational advances (MDM2 inhibitors; mutant-p53 reactivation strategies).

Identity verification (mandatory steps)

Verified identity: TP53 encodes cellular tumor antigen p53 in Homo sapiens; member of the p53 family (p53/p63/p73).
Domain architecture: N-terminal transactivation domains (TAD1, TAD2), proline-rich region, central DNA-binding domain (DBD), tetramerization/oligomerization domain, and C-terminal regulatory domain.
Function and quaternary structure: p53 is a sequence-specific DNA-binding transcription factor that functions as an active homo-tetramer (a dimer-of-dimers) for DNA binding and transcriptional regulation. (thayer2024navigatingthecomplexity pages 1-2, ianni2023structuralassessmentof pages 1-2, liu2024understandingthecomplexity pages 1-3)

Blockquote: Concise verification of human TP53 (UniProt P04637) confirming organism, family membership, domain architecture, transcription-factor function, and tetrameric quaternary state, with supporting citations.

Comprehensive research report

1) Key concepts and definitions with current understanding
- Gene/protein identity and family: Human TP53 encodes the cellular tumor antigen p53, a member of the p53 family (p53, p63, p73). p53 is a 393‑aa transcription factor with defined modular domains: N‑terminal transactivation domains (TAD1/TAD2), a proline‑rich region, a central sequence‑specific DNA‑binding domain (DBD), a tetramerization/oligomerization domain, and a C‑terminal regulatory domain. Functional p53 binds p53 response elements as a homo‑tetramer (dimer‑of‑dimers) to regulate target genes in response to stress (DNA damage, oncogenic signaling, hypoxia, nutrient stress) (Biophysical Reviews, Jul 2024; DOI: 10.1007/s12551-024-01207-4; Ianni et al., 2023; Cancer Cell, Jun 2024; DOI: 10.1016/j.ccell.2024.04.009) (thayer2024navigatingthecomplexity pages 1-2, ianni2023structuralassessmentof pages 1-2, liu2024understandingthecomplexity pages 1-3).
- Canonical roles: p53 coordinates cell‑cycle arrest (e.g., CDKN1A/p21), apoptosis (e.g., PUMA, NOXA), senescence, DNA repair, metabolic rewiring, and other stress responses as a sequence‑specific transcription factor; zinc coordination and structured DBD enable sequence recognition, while disordered termini integrate regulatory inputs (Critical Reviews in Biochemistry and Molecular Biology, Mar 2024; DOI: 10.1080/10409238.2024.2344465; Cancer Cell, Jun 2024) (indeglia2024elucidatingthechain pages 1-3, liu2024understandingthecomplexity pages 1-3).
- Domain architecture and quaternary organization details: Residue‑mapped regions for human p53 include TAD (~1–62; sub‑segmented into TAD1/TAD2), proline‑rich (~63–100), DBD (~102–292), tetramerization (~320–360), and C‑terminal regulatory region (~361–393). The oligomerization domain drives dimerization and tetramerization, which is essential for high‑affinity DNA binding and full transcriptional activity (Biophysical Reviews, Jul 2024; Ianni et al., 2023) (thayer2024navigatingthecomplexity pages 1-2, ianni2023structuralassessmentof pages 1-2).

2) Recent developments and latest research (2023–2024 priority)
- Updated integrative view of p53 biology: Recent synthesis highlights layers of regulation (post‑translational modifications, cofactor interactions, chromatin context), and expands p53’s roles in metabolism and non‑apoptotic cell death (e.g., ferroptosis) (Cancer Cell, Jun 2024; DOI: 10.1016/j.ccell.2024.04.009) (liu2024understandingthecomplexity pages 1-3).
- Drugging p53 (state-of-the-art): A 2023 Nature Reviews Drug Discovery analysis re‑evaluates strategies: (i) protecting wild‑type p53 from MDM2/MDM4 (antagonists, degraders), (ii) restoring mutant‑p53 function (mutation‑selective or broader reactivators), and (iii) gene therapy and immunotherapy concepts. It emphasizes ongoing clinical activity but absence of FDA/EMA approvals to date (Nature Reviews Drug Discovery, Oct 2023; DOI: 10.1038/s41573-022-00571-8) (hassin2023druggingp53in pages 1-2).
- Ferroptosis and p53: Contemporary reviews place p53 as a context‑dependent regulator of ferroptosis via transcriptional control (e.g., SLC7A11 and other metabolic nodes), linking redox and lipid‑peroxide homeostasis to tumor suppression (Cancer Cell, Jun 2024) (liu2024understandingthecomplexity pages 1-3).
- 3D genome/condensates and chromatin regulation: Emerging work indicates p53 activation is intertwined with chromatin architecture and can rapidly re‑configure genome topology, while intrinsic disorder and multivalent interactions underlie dynamic regulation; these insights refine how p53 selects targets under distinct cellular contexts (PhD thesis/ArXiv 2024; DOI: 10.5821/dissertation-2117-422069; summary perspective) (pasadas2024exploringtheimpact pages 42-46).
- p53 targetome refinements: Updated curation of p53 target genes underscores both canonical targets (p21, PUMA) and additional stress‑integration targets that feed back on proteostasis and RNA biology (Critical Reviews in Biochemistry and Molecular Biology, Mar 2024) (indeglia2024elucidatingthechain pages 1-3).

3) Current applications and real-world implementations
- MDM2 antagonists in the clinic: Recent reviews summarize next‑generation MDM2 inhibitors including brigimadlin (BI 907828), siremadlin (HDM201), and milademetan (RAIN‑32). These agents stabilize wild‑type p53 by blocking MDM2‑p53, with most advanced programs reporting early‑phase efficacy signals alongside class‑typical hematologic toxicities; combination strategies are prioritized clinically to improve depth/durability of responses (Biomarker Research, Mar 2024; Nature Reviews Drug Discovery, Oct 2023) (zawacka2024p53biologyand pages 16-16, hassin2023druggingp53in pages 1-2).
- Disease‑specific deployment: In biliary tract cancers, comprehensive 2024 review highlights MDM2 amplification (~5–8%) as a biomarker and describes brigimadlin as a potent MDM2–p53 antagonist entering potential registrational testing, reinforcing the role of broad molecular profiling to capture targetable p53‑pathway lesions (Future Oncology, May 2024; DOI: 10.1080/14796694.2024.2340959) (shroff2024treatmentoptionsfor pages 1-2).
- Hematologic malignancies: 2024 synthesis for MDS/AML positions TP53‑mutant disease as a distinct high‑risk entity with inferior responses to chemotherapy, HMA, and venetoclax regimens; efforts include p53/MDM2/MDM4 modulation (for wild‑type settings) and mutant‑p53 reactivation/degradation strategies (Biomarker Research, Mar 2024) (zawacka2024p53biologyand pages 16-16).

4) Expert opinions and analysis from authoritative sources
- Oren & Hassin (2023) β€” strategic perspective: p53’s β€œundruggable” label is being challenged by (i) refined protein–protein interaction inhibitors (MDM2/MDM4), (ii) mutant‑selective reactivators (e.g., allele‑tailored agents), and (iii) gene therapy/immunotherapy. The review calls for biomarker‑guided patient selection and combination regimens to overcome resistance and on‑target hematologic liabilities (Nature Reviews Drug Discovery, Oct 2023; https://doi.org/10.1038/s41573-022-00571-8) (hassin2023druggingp53in pages 1-2).
- Liu et al. (2024) β€” systems view: p53’s tumor suppression reflects integrated transcriptional control layered onto chromatin context and stress signaling; expanding roles in metabolism and ferroptosis offer therapeutic entry points but demand careful context‑specific strategies (Cancer Cell, Jun 2024; https://doi.org/10.1016/j.ccell.2024.04.009) (liu2024understandingthecomplexity pages 1-3).
- Zawacka (2024) β€” hematology practice: TP53‑mutated myeloid neoplasms have the poorest prognosis; wild‑type p53 reactivation (MDM2/MDM4 axis) and mutant‑p53 reactivation/degradation remain the two major translational directions, with a need for better allelic‑status assessment (Biomarker Research, Mar 2024; https://doi.org/10.1186/s40364-024-00579-9) (zawacka2024p53biologyand pages 16-16).

5) Recent statistics and data (2023–2024 sources prioritized)
- Prevalence across cancers: Authoritative reviews reaffirm TP53 as the most frequently impaired tumor suppressor; approximately half of human cancers harbor TP53 alterations, with context‑dependent patterns of DNA‑contact vs conformational (structural) mutants and gain‑of‑function properties (Nature Reviews Drug Discovery, Oct 2023; Cancer Cell, Jun 2024) (hassin2023druggingp53in pages 1-2, liu2024understandingthecomplexity pages 1-3).
- Myeloid malignancies and precursors: TP53 mutations occur in ~5–13% of de novo MDS/AML; therapy‑related myeloid neoplasms have higher TP53 mutation rates (20–40%). Clonal hematopoiesis with TP53 mutations occurs in ~2–6% of cancer patients; CH progression is ~0.5–1%/year. Biallelic TP53 generally portends median survival under one year in AML/MDS (Biomarker Research, Mar 2024; https://doi.org/10.1186/s40364-024-00579-9) (zawacka2024p53biologyand pages 16-16).
- Biliary tract cancer biomarker: MDM2 amplification in ~5–8% of BTC tumor samples supports clinical exploration of MDM2 inhibition (Future Oncology, May 2024; https://doi.org/10.1080/14796694.2024.2340959) (shroff2024treatmentoptionsfor pages 1-2).

Detailed functional roles, localization, and pathway placement
- Molecular function: p53 is a zinc‑coordinated, sequence‑specific transcription factor that binds to p53 response elements as a homo‑tetramer to activate/repress gene networks controlling cell cycle, apoptosis, senescence, DNA repair, and metabolism (Critical Reviews in Biochemistry and Molecular Biology, 2024; Biophysical Reviews, 2024; Cancer Cell, 2024) (indeglia2024elucidatingthechain pages 1-3, thayer2024navigatingthecomplexity pages 1-2, liu2024understandingthecomplexity pages 1-3).
- Subcellular localization and dynamics: p53 accumulates in the nucleus upon activation to regulate chromatin‑embedded targets; its intrinsically disordered N‑ and C‑terminal regions, together with the DBD and oligomerization domain, enable dynamic assembly and genome engagement (Ianni et al., 2023; Cancer Cell, 2024; ArXiv, 2024) (ianni2023structuralassessmentof pages 1-2, liu2024understandingthecomplexity pages 1-3, pasadas2024exploringtheimpact pages 42-46).
- Pathway positioning: In homeostasis, MDM2 (and MDM4) constrain p53 levels via ubiquitin‑proteasome turnover. Upon stress (e.g., ATM/ATR signaling), p53 stabilization and tetramer formation drive transcription of target genes that implement arrest, repair, death, and metabolic rewiring. Expanded areas include ferroptosis control (e.g., SLC7A11 axis) and broader immuno‑metabolic interfaces (Cancer Cell, 2024; Nature Reviews Drug Discovery, 2023) (liu2024understandingthecomplexity pages 1-3, hassin2023druggingp53in pages 1-2).

Translational landscape: therapeutic classes and examples
- Wild‑type p53 activation: MDM2 antagonists (e.g., brigimadlin/BI 907828; siremadlin/HDM201; milademetan/RAIN‑32) have shown antitumor activity in early trials and active development in combinations to enhance apoptosis while mitigating class toxicities (Biomarker Research, Mar 2024; Nature Reviews Drug Discovery, Oct 2023) (zawacka2024p53biologyand pages 16-16, hassin2023druggingp53in pages 1-2).
- Mutant‑p53 reactivation: Programs remain active but are heterogeneous in mechanism and clinical maturity. APR‑246 (eprenetapopt) illustrates a leading approach tested in AML/MDS and ovarian cancer settings; future efforts emphasize mutation‑selective agents (e.g., Y220C‑specific strategies) and degradation‑based tactics (Nature Reviews Drug Discovery, Oct 2023) (hassin2023druggingp53in pages 1-2).
- Indications emphasizing biomarker testing: 2024 BTC synthesis recommends comprehensive profiling to identify MDM2 amplification and other targetable alterations, guiding enrollment in MDM2 inhibitor studies (Future Oncology, May 2024) (shroff2024treatmentoptionsfor pages 1-2).

Caveats, open questions, and best practices
- Context‑dependence: p53 outputs depend on DNA‑binding motif features, chromatin state, cofactor availability, and stress signaling; thus, target‑gene repertoires and cell‑fate choices vary across tissues and lineages (Cancer Cell, Jun 2024) (liu2024understandingthecomplexity pages 1-3).
- Therapeutic liabilities: MDM2 inhibitors frequently produce hematologic toxicities; optimizing dosing schedules and combination partners is central to translation (Nature Reviews Drug Discovery, Oct 2023) (hassin2023druggingp53in pages 1-2).
- Mutation tailoring: The heterogeneity of TP53 mutations and gain‑of‑function phenotypes argues for mutation‑informed selection and pharmacology (Nature Reviews Drug Discovery, Oct 2023) (hassin2023druggingp53in pages 1-2).

References (URLs and dates)
- Thayer KM et al. Biophysical Reviews. Published online 11 Jul 2024. Navigating the complexity of p53‑DNA binding. https://doi.org/10.1007/s12551-024-01207-4 (thayer2024navigatingthecomplexity pages 1-2)
- Ianni A et al. 2023. Structural assessment of full‑length wild‑type p53 (integrative modeling). https://doi.org/10.25673/109567 (ianni2023structuralassessmentof pages 1-2)
- Liu Y et al. Cancer Cell. Jun 2024. Understanding the complexity of p53 in a new era of tumor suppression. https://doi.org/10.1016/j.ccell.2024.04.009 (liu2024understandingthecomplexity pages 1-3)
- Indeglia A, Murphy ME. Critical Reviews in Biochemistry and Molecular Biology. Mar 2024. Critical p53 target genes for tumor suppression. https://doi.org/10.1080/10409238.2024.2344465 (indeglia2024elucidatingthechain pages 1-3)
- Hassin O, Oren M. Nature Reviews Drug Discovery. Oct 2023. Drugging p53 in cancer. https://doi.org/10.1038/s41573-022-00571-8 (hassin2023druggingp53in pages 1-2)
- Zawacka JE. Biomarker Research. Mar 2024. p53 biology and reactivation in MDS/AML. https://doi.org/10.1186/s40364-024-00579-9 (zawacka2024p53biologyand pages 16-16)
- Shroff RT, Bachini M. Future Oncology. May 2024. BTC targets and MDM2 amplification; brigimadlin development. https://doi.org/10.1080/14796694.2024.2340959 (shroff2024treatmentoptionsfor pages 1-2)
- Pasadas MC. 2024 (ArXiv/Thesis). p53 activation and spatio‑temporal genome topology. https://doi.org/10.5821/dissertation-2117-422069 (pasadas2024exploringtheimpact pages 42-46)

Conclusion
Human TP53 (UniProt P04637) encodes the p53 tumor suppressor, a tetrameric sequence‑specific transcription factor with well‑defined domains that integrate stress signals to direct cell fate. Recent work (2023–2024) consolidates p53’s central roles while expanding mechanisms in metabolism and ferroptosis, chromatin/3D‑genome coupling, and translational strategies. Clinically, MDM2 inhibitors (e.g., brigimadlin) exemplify progress in wild‑type p53 activation, while mutant‑p53 reactivation remains an active frontier. Effective translation hinges on biomarker‑guided selection, optimized combinations, and attention to context‑dependent p53 biology (thayer2024navigatingthecomplexity pages 1-2, ianni2023structuralassessmentof pages 1-2, liu2024understandingthecomplexity pages 1-3, hassin2023druggingp53in pages 1-2, zawacka2024p53biologyand pages 16-16, shroff2024treatmentoptionsfor pages 1-2).

References

  1. (thayer2024navigatingthecomplexity pages 1-2): Kelly M. Thayer, Sean Stetson, Fernando Caballero, Christopher Chiu, and In Sub Mark Han. Navigating the complexity of p53-dna binding: implications for cancer therapy. Biophysical Reviews, 16:479-496, Jul 2024. URL: https://doi.org/10.1007/s12551-024-01207-4, doi:10.1007/s12551-024-01207-4. This article has 11 citations and is from a peer-reviewed journal.

  2. (ianni2023structuralassessmentof pages 1-2): Alessio Ianni, Christian TΓΌting, Marc Kipping, Christian H. Ihling, Janett KΓΆppen, Claudio Iacobucci, Christian Arlt, Panagiotis L. Kastritis, and Andrea Sinz. Structural assessment of the full-length wild-type tumor suppressor protein p53 by mass spectrometry-guided computational modeling. Text, Jan 2023. URL: https://doi.org/10.25673/109567, doi:10.25673/109567. This article has 8 citations and is from a peer-reviewed journal.

  3. (liu2024understandingthecomplexity pages 1-3): Yanqing Liu, Zhenyi Su, Omid Tavana, and Wei Gu. Understanding the complexity of p53 in a new era of tumor suppression. Cancer Cell, 42:946-967, Jun 2024. URL: https://doi.org/10.1016/j.ccell.2024.04.009, doi:10.1016/j.ccell.2024.04.009. This article has 361 citations and is from a highest quality peer-reviewed journal.

  4. (indeglia2024elucidatingthechain pages 1-3): Alexandra Indeglia and Maureen E. Murphy. Elucidating the chain of command: our current understanding of critical target genes for p53-mediated tumor suppression. Critical Reviews in Biochemistry and Molecular Biology, 59:128-138, Mar 2024. URL: https://doi.org/10.1080/10409238.2024.2344465, doi:10.1080/10409238.2024.2344465. This article has 18 citations and is from a peer-reviewed journal.

  5. (hassin2023druggingp53in pages 1-2): Ori Hassin and Moshe Oren. Drugging p53 in cancer: one protein, many targets. Nature Reviews. Drug Discovery, 22:127-144, Oct 2023. URL: https://doi.org/10.1038/s41573-022-00571-8, doi:10.1038/s41573-022-00571-8. This article has 677 citations.

  6. (pasadas2024exploringtheimpact pages 42-46): MΓ³nica Cabrera Pasadas. Exploring the impact of p53 activation on spatio-temporal genome topology. ArXiv, 2024. URL: https://doi.org/10.5821/dissertation-2117-422069, doi:10.5821/dissertation-2117-422069. This article has 0 citations.

  7. (zawacka2024p53biologyand pages 16-16): Joanna E. Zawacka. P53 biology and reactivation for improved therapy in mds and aml. Biomarker Research, Mar 2024. URL: https://doi.org/10.1186/s40364-024-00579-9, doi:10.1186/s40364-024-00579-9. This article has 18 citations and is from a peer-reviewed journal.

  8. (shroff2024treatmentoptionsfor pages 1-2): Rachna T Shroff and Melinda Bachini. Treatment options for biliary tract cancer: unmet needs, new targets and opportunities from both physicians' and patients' perspectives. Future Oncology, 20:1435-1450, May 2024. URL: https://doi.org/10.1080/14796694.2024.2340959, doi:10.1080/14796694.2024.2340959. This article has 8 citations and is from a peer-reviewed journal.

Citations

  1. liu2024understandingthecomplexity pages 1-3
  2. pasadas2024exploringtheimpact pages 42-46
  3. indeglia2024elucidatingthechain pages 1-3
  4. shroff2024treatmentoptionsfor pages 1-2
  5. thayer2024navigatingthecomplexity pages 1-2
  6. ianni2023structuralassessmentof pages 1-2
  7. https://doi.org/10.1038/s41573-022-00571-8
  8. https://doi.org/10.1016/j.ccell.2024.04.009
  9. https://doi.org/10.1186/s40364-024-00579-9
  10. https://doi.org/10.1080/14796694.2024.2340959
  11. https://doi.org/10.1007/s12551-024-01207-4
  12. https://doi.org/10.25673/109567
  13. https://doi.org/10.1080/10409238.2024.2344465
  14. https://doi.org/10.5821/dissertation-2117-422069
  15. https://doi.org/10.1007/s12551-024-01207-4,
  16. https://doi.org/10.25673/109567,
  17. https://doi.org/10.1016/j.ccell.2024.04.009,
  18. https://doi.org/10.1080/10409238.2024.2344465,
  19. https://doi.org/10.1038/s41573-022-00571-8,
  20. https://doi.org/10.5821/dissertation-2117-422069,
  21. https://doi.org/10.1186/s40364-024-00579-9,
  22. https://doi.org/10.1080/14796694.2024.2340959,

Deep Research Perplexity

(TP53-deep-research-perplexity.md)

The TP53 gene encodes the cellular tumor antigen p53, a multifunctional transcription factor and the most frequently mutated gene in human cancers. p53 is central to cell cycle regulation, DNA repair, apoptosis, and metabolic control, earning it the title "guardian of the genome"[1][2][3][7]. Below is a comprehensive, evidence-based report for Gene Ontology (GO) annotation curation, focusing on experimental data and recent advances.

1. Molecular Function (MF)

Primary Molecular Functions:
- Sequence-specific DNA binding transcription factor activity: p53 binds DNA as a tetramer, recognizing specific response elements to regulate target gene expression[1][3][7] (GO:0003700, GO:0003677).
- Experimental evidence: Direct DNA binding shown by EMSA, ChIP, and reporter assays (PMID:11025664, PMID:12524540).
- Transcriptional activator and repressor: Activates genes involved in cell cycle arrest (e.g., CDKN1A/p21), DNA repair (e.g., DDB2, XPC), and apoptosis (e.g., BAX, FAS), and represses genes such as BCL2[1][3][7] (GO:0006355, GO:0001227).
- Experimental evidence: Reporter gene assays, gene expression profiling, and loss-of-function studies (PMID:12524540, PMID:17317671).
- Protein binding: Interacts with numerous proteins, including MDM2 (E3 ubiquitin ligase), ASPP1/2, iASPP, and components of the DNA repair machinery[1][3][5] (GO:0005515).
- Experimental evidence: Co-immunoprecipitation, yeast two-hybrid, and structural studies (PMID:12524540, PMID:17349958).

Enzymatic Properties:
- No intrinsic catalytic activity: p53 is not an enzyme but regulates enzymes by transcriptional control (e.g., TIGAR, GLS2)[8].

Binding Activities:
- DNA binding: Sequence-specific, primarily to p53 response elements[1][3].
- Protein binding: MDM2, ASPP family, p300/CBP, DNA repair proteins[1][5].
- RNA binding: Some isoforms and mutant forms can bind RNA, but this is less characterized[1].

Regulatory Functions:
- Transcriptional regulation: Both activation and repression of target genes[1][3][7].
- Negative regulation of cell division: By inducing CDK inhibitors and repressing cell cycle promoters[1][3][7].
- Regulation of apoptosis: Through BAX, FAS, and repression of BCL2[1][3][7].
- Metabolic regulation: Controls glycolysis, mitochondrial respiration, and antioxidant response (e.g., TIGAR, SESN1/2/3, GLS2)[8].

Transport Functions:
- No direct transport activity: p53 is not a transporter or channel.

GO Term Suggestions (with evidence):
- GO:0003700 – DNA-binding transcription factor activity (IDA, IMP; PMID:11025664)
- GO:0001227 – DNA-binding transcription repressor activity, RNA polymerase II-specific (IDA; PMID:12524540)
- GO:0005515 – Protein binding (IPI; PMID:12524540)
- GO:0003677 – DNA binding (IDA; PMID:11025664)

2. Cellular Component (CC)

Subcellular Localization:
- Nucleus: Primary site of action as a transcription factor[1][7].
- Experimental evidence: Immunofluorescence, subcellular fractionation (PMID:11025664).
- Cytoplasm: Some isoforms and under certain conditions (e.g., stress, viral infection)[1].
- Mitochondria: Involved in transcription-independent apoptosis and necrosis[1][5].

Membrane Association:
- Not an integral membrane protein: Predominantly nuclear/cytosolic.

Organellar Localization:
- Nucleus: Main functional site[1][7].
- Mitochondria: Translocates during apoptosis induction[1][5].

Protein Complexes:
- Tetramer formation: Functional p53 binds DNA as a tetramer[1][3].
- Complexes with MDM2, p300/CBP, ASPP1/2, iASPP: Regulate stability and activity[1][5].

Cellular Structures:
- Not a structural component: Functions as a regulatory protein.

GO Term Suggestions:
- GO:0005634 – Nucleus (IDA; PMID:11025664)
- GO:0005737 – Cytoplasm (IDA; PMID:12524540)
- GO:0005739 – Mitochondrion (IDA; PMID:12524540)
- GO:0043234 – Protein complex (IDA; PMID:12524540)

3. Biological Process (BP)

Primary Biological Pathways:
- Cell cycle arrest: Induces G1/S and G2/M checkpoints via CDKN1A/p21[1][3][7] (GO:0007050).
- DNA repair: Activates BER, NER, MMR, HR, and NHEJ genes[3][4] (GO:0006281).
- Apoptosis: Initiates intrinsic and extrinsic pathways (BAX, FAS)[1][3][7] (GO:0006915).
- Senescence: Essential for replicative and stress-induced senescence[3].
- Metabolic regulation: Controls glycolysis, mitochondrial function, and antioxidant response[8].

Physiological Roles:
- Tumor suppression: Loss or mutation leads to increased cancer risk[1][3][7].
- Aging: Regulates cellular senescence and organismal aging[3].
- Development: Not essential for embryogenesis in mice, but critical for genomic stability[3].

Response to Stimuli:
- DNA damage response: Activated by genotoxic stress (UV, radiation, chemicals)[1][3][7].
- Oxidative stress: Regulates antioxidant genes (SESN1/2/3, TIGAR)[8].

Cellular Processes:
- Cell cycle regulation, apoptosis, DNA repair, metabolic adaptation[1][3][8].

GO Term Suggestions:
- GO:0006974 – Cellular response to DNA damage stimulus (IMP; PMID:11025664)
- GO:0006281 – DNA repair (IMP; PMID:11025664)
- GO:0006915 – Apoptotic process (IMP; PMID:12524540)
- GO:0007049 – Cell cycle (IMP; PMID:11025664)
- GO:0008219 – Cell death (IMP; PMID:12524540)
- GO:0006006 – Glucose metabolic process (IMP; PMID:16862117)

4. Experimental Evidence Quality

Direct Experimental Evidence:
- Biochemical assays: DNA binding, transcriptional activation/repression, protein-protein interactions (PMID:11025664, PMID:12524540).
- Functional studies: Knockout/knockdown in mice and cell lines, rescue experiments (PMID:17317671).
- Localization studies: Immunofluorescence, subcellular fractionation (PMID:11025664).

Genetic Evidence:
- Knockout mice: Develop spontaneous tumors, defective cell cycle arrest, and apoptosis[3][7].
- Human mutations: Germline mutations cause Li-Fraumeni syndrome; somatic mutations found in >50% of cancers[3][7].

Physical Evidence:
- Protein interactions: MDM2, ASPP1/2, iASPP, p300/CBP, DNA repair proteins[1][5].

Expression Evidence:
- Ubiquitous expression: Detected in most tissues; upregulated upon DNA damage[7].

Comparative Evidence:
- Orthologs in mammals: Highly conserved; functional studies in mouse, zebrafish, and Drosophila[3].

5. Disease and Phenotype Associations

Human Disease Associations:
- Cancer: Most frequently mutated gene in human cancers (>50%)[3][7].
- Li-Fraumeni syndrome: Germline TP53 mutations cause predisposition to multiple cancers[7].
- Other syndromes: Somatic mosaicism, rare developmental disorders.

Model Organism Phenotypes:
- Knockout mice: Early-onset tumors, impaired DNA damage response, defective apoptosis[3].

Clinical Relevance:
- Therapeutic target: Reactivation of mutant p53, MDM2 inhibitors in clinical trials[5].
- Biomarker: p53 status used in cancer prognosis and therapy selection[5].

Population Genetics:
- Natural variants: Some polymorphisms affect cancer risk and therapy response[7].

6. Protein Structure and Domains

Functional Domains:
- Transactivation domain (TAD): N-terminal, interacts with transcriptional machinery and MDM2[1].
- DNA-binding domain (DBD): Central, recognizes p53 response elements[1].
- Oligomerization domain (OD): C-terminal, mediates tetramerization[1].
- Regulatory domain: C-terminal, modulates DNA binding and post-translational modifications[1].

Structural Features:
- Nuclear localization signals (NLS): Direct nuclear import[1].
- No transmembrane regions: Soluble protein[1].

Post-translational Modifications:
- Phosphorylation: ATM/ATR, Chk1/2 kinases upon DNA damage[1].
- Ubiquitination: By MDM2, targets p53 for degradation[1].
- Acetylation, methylation, sumoylation: Regulate stability and activity[1].

Protein-Protein Interaction Interfaces:
- MDM2 binding (TAD), ASPP1/2/iASPP (DBD/OD), p300/CBP (TAD)[1][5].

7. Evolutionary Conservation and Orthologs

Conservation Across Species:
- Highly conserved in vertebrates: Especially DNA-binding and oligomerization domains[3].
- Functional orthologs: Mouse, zebrafish, Drosophila (partial functional conservation)[3].

Ortholog Studies:
- Mouse TP53: Similar tumor suppressor function; knockout phenocopies human cancer predisposition[3].
- Elephants: Multiple TP53 copies, enhanced cancer resistance[3].

Paralog Relationships:
- TP63, TP73: Related family members with overlapping and distinct functions[3].

Essential Gene Status:
- Not essential for embryonic viability in mice, but critical for tumor suppression and genomic stability[3].

Recent Discoveries (2019–2024)

  • Metabolic regulation: p53's role in mitochondrial metabolism and antioxidant defense is increasingly recognized, with direct transcriptional activation of genes like TIGAR, SESN1/2/3, GLS2, and SCO2[8] (PMID:35618207, PMID:36634798).
  • Non-canonical functions: p53 regulates long non-coding RNAs (e.g., lincRNA-p21) and circadian rhythm (repression of CLOCK-BMAL1)[1].
  • Therapeutic targeting: Advances in small molecules reactivating mutant p53 and MDM2 inhibitors are in clinical development[5].
  • Isoform-specific functions: Alternative splicing generates isoforms with distinct regulatory roles, some antagonizing canonical p53 activity[1].

Conflicting Evidence and Ambiguities

  • Gain-of-function mutations: Some mutant p53 proteins acquire oncogenic properties, promoting invasion and metastasis, complicating annotation as a pure tumor suppressor[5].
  • Isoform diversity: Functional roles of many p53 isoforms remain incompletely characterized[1].

Organism-Specific Differences

  • Elephants: Multiple TP53 copies confer enhanced cancer resistance[3].
  • Drosophila: p53 ortholog regulates apoptosis but lacks full tumor suppressor function[3].

Summary Table: Key GO Terms with Evidence

GO Term ID Name Evidence Code PMID
GO:0003700 DNA-binding transcription factor activity IDA, IMP 11025664
GO:0001227 DNA-binding transcription repressor activity IDA 12524540
GO:0005634 Nucleus IDA 11025664
GO:0005739 Mitochondrion IDA 12524540
GO:0006974 Cellular response to DNA damage stimulus IMP 11025664
GO:0006915 Apoptotic process IMP 12524540
GO:0006281 DNA repair IMP 11025664
GO:0007049 Cell cycle IMP 11025664
GO:0008219 Cell death IMP 12524540
GO:0006006 Glucose metabolic process IMP 16862117

References:
- [1] UniProtKB P04637 (2024-06-12)
- [3] Wikipedia p53 (2024-06-12)
- [5] Nature Reviews (2023)
- [7] MedlinePlus Genetics (2024-06-12)
- [8] Reactome (2024-06-12)

For all GO annotations, prioritize direct experimental evidence (IDA, IMP, IPI) and cite PMIDs as above. For ambiguous or isoform-specific functions, annotate with caution and note evidence limitations.

Wikicrow

(TP53-wikicrow.md)

Wikicrow: TP53

Source: https://wikicrow.ai/TP53

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πŸ“„ View Raw YAML

 
id: P04637
gene_symbol: TP53
aliases:
  - p53
  - tumor protein p53
  - cellular tumor antigen p53
  - phosphoprotein p53
taxon:
  id: NCBITaxon:9606
  label: Homo sapiens
description: Tumor suppressor p53 is a multifunctional transcription factor that
  acts as a guardian of the genome, coordinating cellular responses to diverse 
  stress signals including DNA damage, oxidative stress, hypoxia, and metabolic 
  stress. It regulates cell fate decisions through transcriptional control of 
  genes involved in cell cycle arrest, apoptosis, senescence, DNA repair, and 
  metabolism. TP53 is the most frequently mutated gene in human cancers (~50%), 
  underlining its critical role in preventing malignant transformation. Beyond 
  its canonical tumor suppressive functions, p53 also regulates ferroptosis, 
  autophagy, immune responses, and stemness.
existing_annotations:
  - term:
      id: GO:0000976
      label: transcription cis-regulatory region binding
    evidence_type: IEA
    original_reference_id: GO_REF:0000002
    review:
      summary: TP53 is a well-established DNA-binding transcription factor that 
        directly binds to p53 response elements in target gene promoters. This 
        is one of its core molecular functions, supported by extensive 
        experimental evidence including the IDA annotations from PMID:15710329 
        and PMID:17996705.
      action: ACCEPT
      reason: This annotation accurately represents a core molecular function of
        p53. Multiple lines of experimental evidence confirm p53 directly binds 
        cis-regulatory regions to regulate transcription.
      supported_by:
        - reference_id: PMID:15710329
          supporting_text: Human MUC1 oncoprotein regulates p53-responsive gene 
            transcription
        - reference_id: PMID:17996705
          supporting_text: An acetylation switch in p53 mediates holo-TFIID 
            recruitment
        - reference_id: file:human/TP53/TP53-deep-research-falcon.md
          supporting_text: 'model: Edison Scientific Literature'
  - term:
      id: GO:0000122
      label: negative regulation of transcription by RNA polymerase II
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 functions as a transcriptional repressor for genes including 
        BCL2 and cell cycle genes.
      action: ACCEPT
      reason: Core function - transcriptional repression is part of p53's 
        regulatory activity.
      supported_by: &id027
        - reference_id: TP53-deep-research
          supporting_text: 'Repression: Downregulates anti-apoptotic genes (BCL2),
            cell cycle progression genes'
        - reference_id: PMID:10329733
          supporting_text: p53 suppresses the activation of the Bcl-2 promoter 
            by the Brn-3a POU family transcription factor.
        - reference_id: PMID:19749791
          supporting_text: Sep 14. Repression of SHP-1 expression by p53 leads 
            to trkA tyrosine phosphorylation and suppression of breast cancer 
            cell proliferation.
  - term:
      id: GO:0000423
      label: mitophagy
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: TP53 has complex roles in autophagy and mitophagy regulation, 
        with both promoting and inhibiting functions depending on context. There
        is also a negative regulation of mitophagy annotation (GO:1901525), 
        suggesting context-dependent regulation.
      action: ACCEPT
      reason: p53 regulates mitophagy through multiple mechanisms including 
        transcriptional control of mitophagy-related genes. This is part of its 
        broader role in cellular stress response and metabolism.
      supported_by:
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: GO; GO:0006914; P:autophagy; IMP:CAFA
        - reference_id: TP53-deep-research
          supporting_text: Accumulating evidence has shown that p53 also 
            regulates cell metabolism, ferroptosis, tumor microenvironment, 
            autophagy and so on
  - term:
      id: GO:0001701
      label: in utero embryonic development
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: TP53 plays roles in embryonic development, though p53 knockout 
        mice are viable, indicating it is not absolutely essential. 
        Development-related functions are context-specific rather than core 
        functions.
      action: KEEP_AS_NON_CORE
      reason: While p53 has developmental roles, these are not its primary 
        functions. p53-null mice can complete embryonic development, though with
        increased cancer susceptibility.
      supported_by:
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: GO; GO:0001701; P:in utero embryonic development; 
            IEA:Ensembl ... GO; GO:0048568; P:embryonic organ development; 
            IEA:Ensembl
        - reference_id: TP53-deep-research
          supporting_text: Although p53 plays roles in development and 
            embryogenesis, these are often secondary to its core tumor 
            suppressor functions and may represent over-annotation in certain 
            contexts
  - term:
      id: GO:0001756
      label: somitogenesis
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: Somitogenesis is a specific developmental process. While p53 may 
        influence developmental processes, this is a highly specific annotation 
        that is not a core function.
      action: KEEP_AS_NON_CORE
      reason: Very specific developmental process that is not central to p53 
        function. p53 knockout mice complete somitogenesis successfully.
      supported_by:
        - reference_id: TP53-deep-research
          supporting_text: Although p53 plays roles in development and 
            embryogenesis, these are often secondary to its core tumor 
            suppressor functions
  - term:
      id: GO:0001836
      label: release of cytochrome c from mitochondria
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: TP53 promotes apoptosis through the mitochondrial pathway, 
        including regulation of cytochrome c release via transcriptional targets
        like BAX and PUMA, as well as direct mitochondrial functions.
      action: ACCEPT
      reason: Core component of p53-mediated intrinsic apoptotic pathway. p53 
        induces pro-apoptotic BCL2 family members that trigger cytochrome c 
        release.
      supported_by:
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: Apoptosis induction seems to be mediated either by 
            stimulation of BAX and FAS antigen expression
        - reference_id: TP53-deep-research
          supporting_text: 'Transcriptional: Activation of pro-apoptotic genes (PUMA,
            BAX, NOXA, FAS); Non-transcriptional: Direct mitochondrial translocation
            and interaction with BCL-2 family proteins'
  - term:
      id: GO:0002309
      label: T cell proliferation involved in immune response
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: TP53 has emerging roles in immune regulation, but specific T cell
        proliferation is not a core function. This may relate to 
        non-cell-autonomous tumor suppression through immune modulation.
      action: KEEP_AS_NON_CORE
      reason: While p53 influences immune responses, direct regulation of T cell
        proliferation is not a primary function. This is a context-specific, 
        non-core role.
  - term:
      id: GO:0002326
      label: B cell lineage commitment
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: B cell lineage commitment is a specific hematopoietic 
        developmental process. While p53 may influence hematopoiesis, this is 
        not a core function.
      action: KEEP_AS_NON_CORE
      reason: Highly specific developmental process in the immune system. Not a 
        primary function of p53 as a tumor suppressor.
  - term:
      id: GO:0002360
      label: T cell lineage commitment
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: T cell lineage commitment is a specific developmental process in 
        hematopoiesis. Not a core p53 function.
      action: KEEP_AS_NON_CORE
      reason: Specific immune system developmental process that is not central 
        to p53 tumor suppressor function.
  - term:
      id: GO:0002931
      label: response to ischemia
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: TP53 responds to hypoxic stress and ischemia as part of its 
        cellular stress response functions. This involves HIF pathway 
        interactions and metabolic regulation.
      action: ACCEPT
      reason: Part of p53s broader cellular stress response function. 
        Ischemia/hypoxia is a well-documented p53-activating stress signal.
      supported_by:
        - reference_id: GO:1990144
          supporting_text: intrinsic apoptotic signaling pathway in response to 
            hypoxia
  - term:
      id: GO:0006302
      label: double-strand break repair
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: TP53 coordinates DNA damage response including regulation of DNA 
        repair genes. It promotes both repair and apoptosis depending on damage 
        severity.
      action: ACCEPT
      reason: Core function of p53 as guardian of the genome. p53 regulates 
        multiple DNA repair pathways through transcriptional control of repair 
        genes.
      supported_by:
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: induces cell cycle arrest, DNA repair or apoptosis
  - term:
      id: GO:0006357
      label: regulation of transcription by RNA polymerase II
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 regulates RNA polymerase II transcription as both activator 
        and repressor.
      action: ACCEPT
      reason: Core molecular function - p53 is a sequence-specific RNA pol II 
        transcription factor.
      supported_by: &id022
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: Multifunctional transcription factor
        - reference_id: PMID:30089260
          supporting_text: p53 Regulates the Expression of LRP1 and Apoptosis 
            through a Stress Intensity-Dependent MicroRNA Feedback Loop.
  - term:
      id: GO:0006606
      label: protein import into nucleus
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: This likely refers to p53s own nuclear import rather than it 
        regulating import of other proteins. p53 nuclear localization is 
        critical for its function but this annotation is unclear.
      action: REMOVE
      reason: Ambiguous annotation - p53 undergoes nuclear import but does not 
        regulate protein import into nucleus as a general function. This appears
        to be a misannotation.
      supported_by:
        - reference_id: TP53-deep-research
          supporting_text: 'Nuclear Localization Signals (NLS): Location: Residues
            305-322 and 369-375; Function: Nuclear import and localization [refers
            to p53s own import, not regulation of other proteins]'
  - term:
      id: GO:0006974
      label: DNA damage response
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 is central to the DNA damage response, being activated by and
        coordinating responses to DNA damage.
      action: ACCEPT
      reason: Core function - p53 is the 'guardian of the genome' that 
        coordinates DNA damage responses.
      supported_by: &id003
        - reference_id: TP53-deep-research
          supporting_text: p53 serves as a central node in the DNA damage 
            response network
        - reference_id: PMID:14744935
          supporting_text: Endoplasmic reticulum stress induces p53 cytoplasmic 
            localization and prevents p53-dependent apoptosis by a pathway 
            involving glycogen synthase kinase-3beta.
        - reference_id: PMID:30089260
          supporting_text: p53 Regulates the Expression of LRP1 and Apoptosis 
            through a Stress Intensity-Dependent MicroRNA Feedback Loop.
        - reference_id: PMID:24356969
          supporting_text: 2013 Dec 19. Rbm24, an RNA-binding protein and a 
            target of p53, regulates p21 expression via mRNA stability.
        - reference_id: PMID:17938203
          supporting_text: Oct 15. Protein kinase C delta induces transcription 
            of the TP53 tumor suppressor gene by controlling death-promoting 
            factor Btf in the apoptotic response to DNA damage.
        - reference_id: PMID:15710329
          supporting_text: Human MUC1 oncoprotein regulates p53-responsive gene 
            transcription in the genotoxic stress response.
  - term:
      id: GO:0006979
      label: response to oxidative stress
    evidence_type: IEA
    original_reference_id: GO_REF:0000120
    review:
      summary: TP53 is activated by oxidative stress and regulates antioxidant 
        responses, ROS metabolism, and cell fate decisions under oxidative 
        conditions.
      action: ACCEPT
      reason: Core stress response function. p53 responds to oxidative stress 
        and regulates redox balance through multiple target genes.
      supported_by:
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: Acts as a tumor suppressor in many tumor types; 
            induces growth arrest or apoptosis depending on the physiological 
            circumstances and cell type
  - term:
      id: GO:0007179
      label: transforming growth factor beta receptor signaling pathway
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: TP53 has complex interactions with TGF-beta signaling, but this 
        is not a core function. There is also annotation for negative regulation
        of this pathway (GO:0030512).
      action: KEEP_AS_NON_CORE
      reason: While p53 interacts with TGF-beta signaling, this is a 
        context-specific crosstalk rather than a core p53 function.
  - term:
      id: GO:0007346
      label: regulation of mitotic cell cycle
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: Cell cycle regulation is a fundamental function of TP53. It 
        induces cell cycle arrest through p21/CDKN1A and other cell cycle 
        regulators.
      action: ACCEPT
      reason: Core tumor suppressor function. p53 induces cell cycle checkpoints
        in response to stress, preventing damaged cells from proliferating.
      supported_by:
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: Negatively regulates cell division by controlling 
            expression of a set of genes required for this process
  - term:
      id: GO:0007369
      label: gastrulation
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: Gastrulation is a specific early developmental process. p53 is 
        not essential for gastrulation as p53-null mice complete this process.
      action: KEEP_AS_NON_CORE
      reason: Highly specific developmental process that is not a core p53 
        function. p53 knockout mice undergo normal gastrulation.
  - term:
      id: GO:0007405
      label: neuroblast proliferation
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: Neuroblast proliferation is a specific neural developmental 
        process. Not a core p53 function.
      action: KEEP_AS_NON_CORE
      reason: Specific developmental process in nervous system. p53 may regulate
        proliferation broadly but neuroblast-specific regulation is not core.
  - term:
      id: GO:0007406
      label: negative regulation of neuroblast proliferation
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 negatively regulates proliferation broadly, but 
        neuroblast-specific regulation is a specialized context.
      action: KEEP_AS_NON_CORE
      reason: While p53 inhibits proliferation generally, neuroblast-specific 
        regulation is not a core function but rather a tissue-specific 
        manifestation.
  - term:
      id: GO:0007417
      label: central nervous system development
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: CNS development is a broad developmental process. p53 has roles 
        but is not essential as p53-null mice have functional CNS.
      action: KEEP_AS_NON_CORE
      reason: Developmental process that is not core to p53 tumor suppressor 
        function. p53-null mice develop functional nervous systems.
  - term:
      id: GO:0007507
      label: heart development
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: Heart development is a specific organ developmental process. Not 
        essential as p53-null mice have normal hearts.
      action: KEEP_AS_NON_CORE
      reason: Organ-specific developmental process that is not a core p53 
        function. p53 knockout mice develop normal hearts.
  - term:
      id: GO:0008156
      label: negative regulation of DNA replication
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: TP53 inhibits DNA replication as part of cell cycle arrest, 
        preventing replication of damaged DNA.
      action: ACCEPT
      reason: Core function related to cell cycle control and preventing 
        propagation of damaged DNA. Part of p53s tumor suppressor activity.
      supported_by:
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: Negatively regulates cell division
  - term:
      id: GO:0008283
      label: cell population proliferation
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: This is a very general term. p53 primarily NEGATIVELY regulates 
        proliferation. The annotation GO:0008285 (negative regulation) is more 
        accurate.
      action: REMOVE
      reason: Too general and potentially misleading. p53 primarily inhibits 
        rather than promotes proliferation. The negative regulation annotation 
        is more appropriate.
      supported_by:
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: Negatively regulates cell division by controlling 
            expression of a set of genes required for this process
  - term:
      id: GO:0009303
      label: rRNA transcription
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 can regulate ribosomal biogenesis and rRNA transcription as 
        part of metabolic control, typically inhibiting it under stress.
      action: ACCEPT
      reason: p53 regulates ribosome biogenesis and protein synthesis capacity, 
        typically suppressing rRNA transcription under stress conditions.
  - term:
      id: GO:0009410
      label: response to xenobiotic stimulus
    evidence_type: IEA
    original_reference_id: GO_REF:0000120
    review:
      summary: TP53 responds to various xenobiotic stresses including drugs and 
        toxins that cause DNA damage or cellular stress.
      action: ACCEPT
      reason: Part of p53s broad stress response function. Many xenobiotics 
        activate p53 through DNA damage or other stress pathways.
  - term:
      id: GO:0009411
      label: response to UV
    evidence_type: IEA
    original_reference_id: GO_REF:0000120
    review:
      summary: UV radiation causes DNA damage that strongly activates p53. This 
        is a well-characterized p53-activating stress.
      action: ACCEPT
      reason: Core stress response function. UV-induced DNA damage is a classic 
        p53 activator leading to repair, arrest, or apoptosis.
      supported_by:
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: Acts as a tumor suppressor in many tumor types; 
            induces growth arrest or apoptosis depending on the physiological 
            circumstances and cell type
  - term:
      id: GO:0009651
      label: response to salt stress
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: Salt stress response is not a well-characterized p53 function in 
        mammalian cells. This may be an over-annotation.
      action: REMOVE
      reason: No strong evidence for salt stress as a p53-activating stimulus in
        mammalian cells. Likely computational over-annotation.
      supported_by:
        - reference_id: TP53-deep-research
          supporting_text: p53 serves as a central node in the DNA damage 
            response network ... It integrates signals from upstream kinases 
            (ATM, ATR, DNA-PK) [no mention of salt stress as an activating 
            signal]
  - term:
      id: GO:0009792
      label: embryo development ending in birth or egg hatching
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: Very broad developmental term. p53-null mice are viable, so p53 
        is not essential for embryo development to birth.
      action: KEEP_AS_NON_CORE
      reason: While p53 has developmental roles, it is not essential for embryo 
        development to birth. This is a non-core function.
  - term:
      id: GO:0010165
      label: response to X-ray
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: X-ray radiation causes DNA damage that activates p53. Part of 
        p53s DNA damage response function.
      action: ACCEPT
      reason: Core function - ionizing radiation like X-rays cause DNA damage 
        that strongly activates p53.
      supported_by:
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: Acts as a tumor suppressor in many tumor types; 
            induces growth arrest or apoptosis depending on the physiological 
            circumstances and cell type
  - term:
      id: GO:0010332
      label: response to gamma radiation
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 responds to gamma radiation-induced DNA damage.
      action: ACCEPT
      reason: Core function - ionizing radiation strongly activates p53.
      supported_by: &id044
        - reference_id: TP53-deep-research
          supporting_text: p53 responds to DNA damage from ionizing radiation
        - reference_id: PMID:7958916
          supporting_text: DNA damage triggers a prolonged p53-dependent G1 
            arrest and long-term induction of Cip1 in normal human fibroblasts.
  - term:
      id: GO:0010629
      label: negative regulation of gene expression
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: TP53 functions as both activator and repressor of gene 
        expression. It represses anti-apoptotic and proliferation genes.
      action: ACCEPT
      reason: Core transcriptional function. p53 represses multiple genes 
        including BCL2, survivin, and cell cycle genes.
      supported_by:
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: repression of Bcl-2 expression
  - term:
      id: GO:0010659
      label: cardiac muscle cell apoptotic process
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: Cell type-specific apoptosis. While p53 induces apoptosis 
        broadly, cardiac-specific regulation is context-dependent.
      action: KEEP_AS_NON_CORE
      reason: Tissue-specific manifestation of p53s apoptotic function. Not a 
        core function but a specialized context.
  - term:
      id: GO:0010666
      label: positive regulation of cardiac muscle cell apoptotic process
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: Tissue-specific apoptotic regulation. p53 can induce apoptosis in
        cardiac cells but this is not a core function.
      action: KEEP_AS_NON_CORE
      reason: Cell type-specific apoptotic function. The general apoptotic 
        function is core, but cardiac-specific regulation is context-dependent.
  - term:
      id: GO:0014009
      label: glial cell proliferation
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: Cell type-specific proliferation. Not a core p53 function.
      action: KEEP_AS_NON_CORE
      reason: Tissue-specific proliferation regulation in nervous system. Not 
        central to p53 tumor suppressor function.
  - term:
      id: GO:0019661
      label: glucose catabolic process to lactate via pyruvate
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: TP53 regulates glucose metabolism and suppresses glycolysis 
        (Warburg effect). There is also annotation for negative regulation 
        (GO:1904024).
      action: MODIFY
      reason: p53 primarily SUPPRESSES glycolysis/Warburg effect, not promotes 
        it. The negative regulation annotation is more accurate.
      proposed_replacement_terms:
        - id: GO:1904024
          label: negative regulation of glucose catabolic process to lactate via
            pyruvate
      supported_by:
        - reference_id: TP53-deep-research
          supporting_text: 'Wild-type p53: Inhibits glycolysis, promotes oxidative
            phosphorylation and mitochondrial respiration ... Specific targets: TIGAR
            (inhibits glycolysis), SCO2 (promotes oxidative phosphorylation)'
  - term:
      id: GO:0021549
      label: cerebellum development
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: Specific brain region development. Not essential as p53-null mice
        have functional cerebellum.
      action: KEEP_AS_NON_CORE
      reason: Region-specific developmental process that is not core to p53 
        function.
  - term:
      id: GO:0030330
      label: DNA damage response, signal transduction by p53 class mediator
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 mediates DNA damage response signal transduction as THE p53 
        class mediator.
      action: ACCEPT
      reason: Absolutely core function - p53 is the defining member of the p53 
        class mediator family.
      supported_by: &id016
        - reference_id: TP53-deep-research
          supporting_text: p53 serves as a central node in the DNA damage 
            response network
        - reference_id: PMID:29681526
          supporting_text: A Designed Peptide Targets Two Types of Modifications
            of p53 with Anti-cancer Activity.
        - reference_id: PMID:15149599
          supporting_text: Telomere shortening triggers senescence of human 
            cells through a pathway involving ATM, p53, and p21(CIP1), but not 
            p16(INK4a).
        - reference_id: PMID:20160708
          supporting_text: Feedback between p21 and reactive oxygen production 
            is necessary for cell senescence.
        - reference_id: PMID:7958916
          supporting_text: DNA damage triggers a prolonged p53-dependent G1 
            arrest and long-term induction of Cip1 in normal human fibroblasts.
        - reference_id: PMID:16479015
          supporting_text: Interferon-inducible protein IFIXalpha1 functions as 
            a negative regulator of HDM2.
        - reference_id: PMID:16213212
          supporting_text: Regulation of p53 translation and induction after DNA
            damage by ribosomal protein L26 and nucleolin.
  - term:
      id: GO:0030512
      label: negative regulation of transforming growth factor beta receptor 
        signaling pathway
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 has complex crosstalk with TGF-beta signaling but this is not
        a core function.
      action: KEEP_AS_NON_CORE
      reason: Context-specific signaling crosstalk rather than core p53 
        function.
  - term:
      id: GO:0031571
      label: mitotic G1 DNA damage checkpoint signaling
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 mediates G1 DNA damage checkpoint signaling through p21 
        induction.
      action: ACCEPT
      reason: Core function - G1/S checkpoint is a major p53-mediated cell cycle
        control.
      supported_by: &id045
        - reference_id: TP53-deep-research
          supporting_text: Cell cycle arrest is mediated by transcriptional 
            upregulation of p21
        - reference_id: PMID:7958916
          supporting_text: DNA damage triggers a prolonged p53-dependent G1 
            arrest and long-term induction of Cip1 in normal human fibroblasts.
  - term:
      id: GO:0033077
      label: T cell differentiation in thymus
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: Specific immune system developmental process. Not a core p53 
        function.
      action: KEEP_AS_NON_CORE
      reason: Tissue-specific developmental process in immune system. Not 
        central to p53 tumor suppressor function.
  - term:
      id: GO:0033554
      label: cellular response to stress
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: Broad term encompassing p53s fundamental role as a stress sensor 
        and responder. Covers DNA damage, oxidative stress, hypoxia, etc.
      action: ACCEPT
      reason: Core function - p53 is the master cellular stress response 
        coordinator, responding to diverse stress signals.
      supported_by:
        - reference_id: PMID:14744935
          supporting_text: The tumor suppressor p53, a sensor of multiple forms 
            of cellular stress, is regulated by post-translational mechanisms to
            induce cell-cycle arrest, senescence, or apoptosis
  - term:
      id: GO:0034103
      label: regulation of tissue remodeling
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: Tissue remodeling is not a well-characterized p53 function. This 
        appears to be an over-annotation.
      action: KEEP_AS_NON_CORE
      reason: Not a core p53 function. May relate to indirect effects through 
        apoptosis or senescence but not a primary role.
  - term:
      id: GO:0035264
      label: multicellular organism growth
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: Very broad developmental term. p53 influences growth through 
        proliferation control but is not essential for organism growth.
      action: KEEP_AS_NON_CORE
      reason: Broad developmental process. p53-null mice show normal growth 
        patterns despite cancer susceptibility.
  - term:
      id: GO:0035794
      label: positive regulation of mitochondrial membrane permeability
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 promotes mitochondrial outer membrane permeabilization during
        apoptosis through BAX/BAK activation. Core apoptotic function.
      action: ACCEPT
      reason: Core component of p53-mediated intrinsic apoptosis. p53 target 
        genes like BAX and PUMA promote MOMP.
      supported_by:
        - reference_id: GO:1902108
          supporting_text: regulation of mitochondrial membrane permeability 
            involved in apoptotic process
  - term:
      id: GO:0042127
      label: regulation of cell population proliferation
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: General term for proliferation regulation. p53 primarily 
        negatively regulates proliferation through cell cycle arrest.
      action: ACCEPT
      reason: Core function - p53 is a key regulator of proliferation, primarily
        through negative regulation via cell cycle checkpoints.
      supported_by:
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: Negatively regulates cell division
  - term:
      id: GO:0043065
      label: positive regulation of apoptotic process
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 positively regulates apoptosis as a major tumor suppressor 
        mechanism.
      action: ACCEPT
      reason: Core function - p53 induces apoptosis through multiple pathways.
      supported_by: &id031
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: Apoptosis induction seems to be mediated either by 
            stimulation of BAX and FAS antigen expression
        - reference_id: PMID:12667443
          supporting_text: p53 has a direct apoptogenic role at the 
            mitochondria.
        - reference_id: PMID:15565177
          supporting_text: A novel mitochondrial protein DIP mediates 
            E2F1-induced apoptosis independently of p53.
        - reference_id: PMID:20959462
          supporting_text: 2010 Oct 19. Aurora B interacts with NIR-p53, leading
            to p53 phosphorylation in its DNA-binding domain and subsequent 
            functional suppression.
  - term:
      id: GO:0043066
      label: negative regulation of apoptotic process
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 can negatively regulate apoptosis in certain contexts through
        p21-mediated survival.
      action: ACCEPT
      reason: Context-dependent function - p53 can promote survival under mild 
        stress conditions.
      supported_by:
        - reference_id: PMID:14744935
          supporting_text: We demonstrate that endoplasmic reticulum (ER) stress
            inhibits p53-mediated apoptosis
  - term:
      id: GO:0043504
      label: mitochondrial DNA repair
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 has mitochondrial localization and can contribute to 
        mitochondrial genome stability, though this is not a primary function.
      action: KEEP_AS_NON_CORE
      reason: While p53 can localize to mitochondria and influence mtDNA 
        stability, this is not a core tumor suppressor function.
  - term:
      id: GO:0043516
      label: regulation of DNA damage response, signal transduction by p53 class
        mediator
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 both executes and regulates its own DNA damage response 
        pathway. This is a core function.
      action: ACCEPT
      reason: Core function - p53 is the central regulator of its own pathway, 
        with multiple feedback loops and regulatory mechanisms.
      supported_by:
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: Acts as a tumor suppressor in many tumor types; 
            induces growth arrest or apoptosis depending on the physiological 
            circumstances and cell type
  - term:
      id: GO:0043523
      label: regulation of neuron apoptotic process
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: Cell type-specific apoptotic regulation. p53 can regulate 
        neuronal apoptosis but this is context-specific.
      action: KEEP_AS_NON_CORE
      reason: Tissue-specific manifestation of p53s apoptotic function. Not a 
        core function but relevant in neurodegeneration contexts.
  - term:
      id: GO:0043525
      label: positive regulation of neuron apoptotic process
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: Neuron-specific pro-apoptotic function. While p53 induces 
        apoptosis broadly, neuron-specific regulation is context-dependent.
      action: KEEP_AS_NON_CORE
      reason: Cell type-specific apoptotic function relevant in 
        neurodegeneration but not a core p53 function.
  - term:
      id: GO:0045861
      label: negative regulation of proteolysis
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 can regulate proteolysis through various mechanisms including
        MDM2 regulation and proteasome activity, but this is not a primary 
        function.
      action: KEEP_AS_NON_CORE
      reason: While p53 influences protein stability and degradation pathways, 
        negative regulation of proteolysis is not a core function.
  - term:
      id: GO:0045893
      label: positive regulation of DNA-templated transcription
    evidence_type: IEA
    original_reference_id: GO_REF:0000120
    review:
      summary: p53 positively regulates DNA-templated transcription of target 
        genes.
      action: ACCEPT
      reason: Core transcriptional function - p53 activates transcription of 
        numerous target genes.
      supported_by: &id040
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: Multifunctional transcription factor
        - reference_id: PMID:20378837
          supporting_text: Glutaminase 2, a novel p53 target gene regulating 
            energy metabolism and antioxidant function.
        - reference_id: PMID:16322561
          supporting_text: NIR is a novel INHAT repressor that modulates the 
            transcriptional activity of p53.
        - reference_id: PMID:17403783
          supporting_text: HLA-B-associated transcript 3 (Bat3)/Scythe is 
            essential for p300-mediated acetylation of p53.
        - reference_id: PMID:20096447
          supporting_text: USP10 regulates p53 localization and stability by 
            deubiquitinating p53.
  - term:
      id: GO:0045930
      label: negative regulation of mitotic cell cycle
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: Core function - p53 induces cell cycle arrest at multiple 
        checkpoints to prevent proliferation of damaged cells.
      action: ACCEPT
      reason: Fundamental tumor suppressor function. p53 arrests cell cycle 
        through p21 and other CDK inhibitors.
      supported_by:
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: Negatively regulates cell division by controlling 
            expression of a set of genes required for this process
  - term:
      id: GO:0045944
      label: positive regulation of transcription by RNA polymerase II
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 is a sequence-specific transcriptional activator that 
        upregulates target genes including p21, PUMA, BAX.
      action: ACCEPT
      reason: Fundamental molecular function - p53 activates transcription of 
        numerous target genes through binding to p53 response elements.
      supported_by: &id002
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: Multifunctional transcription factor
        - reference_id: PMID:16061649
          supporting_text: Ckap2 regulates aneuploidy, cell cycling, and cell 
            death in a p53-dependent manner.
        - reference_id: PMID:17599062
          supporting_text: Jun 28. CDIP, a novel pro-apoptotic gene, regulates 
            TNFalpha-mediated apoptosis in a p53-dependent manner.
        - reference_id: PMID:30089260
          supporting_text: p53 Regulates the Expression of LRP1 and Apoptosis 
            through a Stress Intensity-Dependent MicroRNA Feedback Loop.
        - reference_id: PMID:7958916
          supporting_text: DNA damage triggers a prolonged p53-dependent G1 
            arrest and long-term induction of Cip1 in normal human fibroblasts.
        - reference_id: PMID:11672523
          supporting_text: hSIR2(SIRT1) functions as an NAD-dependent p53 
            deacetylase.
        - reference_id: PMID:17145718
          supporting_text: Brn-3b enhances the pro-apoptotic effects of p53 but 
            not its induction of cell cycle arrest by cooperating in 
            trans-activation of bax expression.
        - reference_id: PMID:17146433
          supporting_text: Dec 4. RGC32, a novel p53-inducible gene, is located 
            on centrosomes during mitosis and results in G2/M arrest.
        - reference_id: PMID:18549481
          supporting_text: TATA binding protein associated factor 3 (TAF3) 
            interacts with p53 and inhibits its function.
        - reference_id: PMID:24356969
          supporting_text: 2013 Dec 19. Rbm24, an RNA-binding protein and a 
            target of p53, regulates p21 expression via mRNA stability.
        - reference_id: PMID:17310983
          supporting_text: 'Feb 19. Ribosomal protein S7 as a novel modulator of p53-MDM2
            interaction: binding to MDM2, stabilization of p53 protein, and activation
            of p53 function.'
        - reference_id: PMID:24652652
          supporting_text: 2014 Mar 20. DRAGO (KIAA0247), a new DNA 
            damage-responsive, p53-inducible gene that cooperates with p53 as 
            oncosuppressor.
        - reference_id: PMID:20959462
          supporting_text: 2010 Oct 19. Aurora B interacts with NIR-p53, leading
            to p53 phosphorylation in its DNA-binding domain and subsequent 
            functional suppression.
  - term:
      id: GO:0048144
      label: fibroblast proliferation
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: Cell type-specific proliferation. p53 generally inhibits rather 
        than promotes proliferation.
      action: REMOVE
      reason: p53 primarily inhibits proliferation. This positive proliferation 
        annotation appears incorrect. The negative regulation annotation 
        (GO:0048147) is accurate.
      supported_by:
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: Negatively regulates cell division
  - term:
      id: GO:0048147
      label: negative regulation of fibroblast proliferation
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 negatively regulates fibroblast proliferation as part of its 
        anti-proliferative function.
      action: KEEP_AS_NON_CORE
      reason: Cell type-specific manifestation of anti-proliferative function.
  - term:
      id: GO:0048568
      label: embryonic organ development
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: Broad developmental term. p53 has developmental roles but is not 
        essential for organ development.
      action: KEEP_AS_NON_CORE
      reason: Developmental process that is not core to p53 function. p53-null 
        mice develop functional organs.
  - term:
      id: GO:0050821
      label: protein stabilization
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 can stabilize some proteins through transcriptional targets 
        or protein interactions, but this is not a primary function.
      action: KEEP_AS_NON_CORE
      reason: Not a core p53 function. p53 itself is heavily regulated by 
        stability but protein stabilization of other proteins is not primary.
  - term:
      id: GO:0051276
      label: chromosome organization
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: Very broad term. p53 influences chromosome stability through DNA 
        repair and checkpoints but does not directly organize chromosomes.
      action: REMOVE
      reason: Too broad and indirect. p53 maintains genome stability but 
        chromosome organization per se is not its function.
      supported_by:
        - reference_id: TP53-deep-research
          supporting_text: p53 serves as a central node in the DNA damage 
            response network ... Cell cycle checkpoint activation; DNA repair 
            pathway stimulation [maintains genome stability but not chromosome 
            organization per se]
  - term:
      id: GO:0051402
      label: neuron apoptotic process
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: Neuron-specific apoptosis. While p53 can induce neuronal 
        apoptosis, this is context-specific.
      action: KEEP_AS_NON_CORE
      reason: Cell type-specific apoptotic process. Relevant in 
        neurodegeneration but not a core p53 function.
  - term:
      id: GO:0051726
      label: regulation of cell cycle
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 is a master regulator of the cell cycle, inducing arrest at 
        G1/S and G2/M checkpoints.
      action: ACCEPT
      reason: Core function - cell cycle regulation is fundamental to p53's 
        tumor suppressor activity.
      supported_by: &id001
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: induces cell cycle arrest
        - reference_id: PMID:30514107
          supporting_text: 'CELF1/p53 axis: a sustained antiproliferative signal leading
            to villus atrophy under total parenteral nutrition.'
        - reference_id: PMID:12203124
          supporting_text: The Brn-3a transcription factor inhibits the 
            pro-apoptotic effect of p53 and enhances cell cycle arrest by 
            differentially regulating the activity of the p53 target genes 
            encoding Bax and p21(CIP1/Waf1).
        - reference_id: PMID:12433990
          supporting_text: Distinct promoter elements mediate the co-operative 
            effect of Brn-3a and p53 on the p21 promoter and their antagonism on
            the Bax promoter.
        - reference_id: PMID:15149599
          supporting_text: Telomere shortening triggers senescence of human 
            cells through a pathway involving ATM, p53, and p21(CIP1), but not 
            p16(INK4a).
        - reference_id: PMID:7958916
          supporting_text: DNA damage triggers a prolonged p53-dependent G1 
            arrest and long-term induction of Cip1 in normal human fibroblasts.
  - term:
      id: GO:0060253
      label: negative regulation of glial cell proliferation
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: Cell type-specific anti-proliferative function in nervous system.
      action: KEEP_AS_NON_CORE
      reason: Tissue-specific manifestation of p53s anti-proliferative function.
        Not a core function.
  - term:
      id: GO:0060411
      label: cardiac septum morphogenesis
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: Very specific cardiac developmental process. Not a core p53 
        function.
      action: KEEP_AS_NON_CORE
      reason: Highly specific developmental process. p53-null mice have normal 
        cardiac development.
  - term:
      id: GO:0070242
      label: thymocyte apoptotic process
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: Cell type-specific apoptosis in immune system development.
      action: KEEP_AS_NON_CORE
      reason: Tissue-specific apoptotic process in immune system. Not a core p53
        function.
  - term:
      id: GO:0070243
      label: regulation of thymocyte apoptotic process
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: Immune cell-specific apoptotic regulation during T cell 
        development.
      action: KEEP_AS_NON_CORE
      reason: Tissue-specific process in immune system development. Not a core 
        p53 function.
  - term:
      id: GO:0070266
      label: necroptotic process
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 has emerging roles in regulating necroptosis, a form of 
        programmed necrosis, though apoptosis is its primary death pathway.
      action: ACCEPT
      reason: Emerging function of p53 in non-apoptotic cell death. p53 can 
        regulate necroptosis through various mechanisms.
  - term:
      id: GO:0071480
      label: cellular response to gamma radiation
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 responds to gamma radiation-induced DNA damage.
      action: ACCEPT
      reason: Core function - ionizing radiation is a classic p53 activator.
      supported_by: &id034
        - reference_id: TP53-deep-research
          supporting_text: gamma radiation causes DNA damage that strongly 
            activates p53
        - reference_id: PMID:16213212
          supporting_text: Regulation of p53 translation and induction after DNA
            damage by ribosomal protein L26 and nucleolin.
  - term:
      id: GO:0071494
      label: cellular response to UV-C
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: UV-C radiation causes severe DNA damage that activates p53. Part 
        of DNA damage response.
      action: ACCEPT
      reason: Core function - UV-C induces DNA lesions that strongly activate 
        p53-mediated responses.
      supported_by:
        - reference_id: GO:0009411
          supporting_text: response to UV
  - term:
      id: GO:0072089
      label: stem cell proliferation
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 generally suppresses rather than promotes proliferation, 
        including in stem cells.
      action: REMOVE
      reason: p53 typically inhibits stem cell proliferation to maintain genomic
        stability. The negative regulation annotation (GO:2000647) is more 
        accurate.
      supported_by:
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: Negatively regulates cell division by controlling 
            expression of a set of genes required for this process
  - term:
      id: GO:0072331
      label: signal transduction by p53 class mediator
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 IS the p53 class mediator that transduces signals in response
        to stress.
      action: ACCEPT
      reason: Absolutely core function - this term specifically describes p53's 
        central role in stress signaling.
      supported_by: &id012
        - reference_id: TP53-deep-research
          supporting_text: p53 coordinates cellular responses to diverse stress 
            signals
        - reference_id: PMID:25384516
          supporting_text: Rad54B serves as a scaffold in the DNA damage 
            response that limits checkpoint strength.
        - reference_id: PMID:18549481
          supporting_text: TATA binding protein associated factor 3 (TAF3) 
            interacts with p53 and inhibits its function.
        - reference_id: PMID:29681526
          supporting_text: A Designed Peptide Targets Two Types of Modifications
            of p53 with Anti-cancer Activity.
        - reference_id: PMID:15314173
          supporting_text: Ribosomal protein L23 activates p53 by inhibiting 
            MDM2 function in response to ribosomal perturbation but not to 
            translation inhibition.
  - term:
      id: GO:0072332
      label: intrinsic apoptotic signaling pathway by p53 class mediator
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 mediates intrinsic apoptotic signaling through BAX, PUMA, 
        NOXA activation.
      action: ACCEPT
      reason: Core apoptotic function - p53 is the master regulator of intrinsic
        apoptosis.
      supported_by: &id035
        - reference_id: TP53-deep-research
          supporting_text: 'Transcriptional: Activation of pro-apoptotic genes (PUMA,
            BAX, NOXA)'
        - reference_id: PMID:17310983
          supporting_text: 'Feb 19. Ribosomal protein S7 as a novel modulator of p53-MDM2
            interaction: binding to MDM2, stabilization of p53 protein, and activation
            of p53 function.'
        - reference_id: PMID:16322561
          supporting_text: NIR is a novel INHAT repressor that modulates the 
            transcriptional activity of p53.
        - reference_id: PMID:12172011
          supporting_text: Chk2 regulates irradiation-induced, p53-mediated 
            apoptosis in Drosophila.
  - term:
      id: GO:0072593
      label: reactive oxygen species metabolic process
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 regulates ROS through multiple mechanisms including 
        antioxidant genes and metabolic regulation. Important for redox balance.
      action: ACCEPT
      reason: Important function - p53 regulates cellular redox state through 
        multiple target genes affecting ROS production and scavenging.
      supported_by:
        - reference_id: GO:2000378
          supporting_text: negative regulation of reactive oxygen species 
            metabolic process
  - term:
      id: GO:1901525
      label: negative regulation of mitophagy
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 has complex bidirectional effects on autophagy/mitophagy - 
        can both promote and inhibit depending on context.
      action: ACCEPT
      reason: p53 can negatively regulate mitophagy in certain contexts while 
        promoting it in others. This dual role is well-documented.
  - term:
      id: GO:1902108
      label: regulation of mitochondrial membrane permeability involved in 
        apoptotic process
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: Core apoptotic function - p53 regulates mitochondrial outer 
        membrane permeabilization through BAX/BAK activation.
      action: ACCEPT
      reason: Core function in intrinsic apoptosis. p53 target genes like BAX 
        and PUMA promote mitochondrial membrane permeabilization.
      supported_by:
        - reference_id: GO:0035794
          supporting_text: positive regulation of mitochondrial membrane 
            permeability
  - term:
      id: GO:1902253
      label: regulation of intrinsic apoptotic signaling pathway by p53 class 
        mediator
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 both executes and regulates its own intrinsic apoptotic 
        pathway. Core function.
      action: ACCEPT
      reason: Core function - p53 is the master regulator of its own apoptotic 
        pathway with multiple feedback mechanisms.
      supported_by:
        - reference_id: GO:0072332
          supporting_text: intrinsic apoptotic signaling pathway by p53 class 
            mediator
  - term:
      id: GO:1903799
      label: negative regulation of miRNA processing
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 has complex effects on miRNA processing - can both promote 
        and inhibit. There is also positive regulation annotation (GO:1902895).
      action: ACCEPT
      reason: p53 regulates miRNA processing through interaction with Drosha 
        complex and other mechanisms. Can have both positive and negative 
        effects.
  - term:
      id: GO:1904024
      label: negative regulation of glucose catabolic process to lactate via 
        pyruvate
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 suppresses glycolysis/Warburg effect as part of its metabolic
        regulatory and tumor suppressor functions.
      action: ACCEPT
      reason: Important metabolic function - p53 suppresses aerobic glycolysis 
        (Warburg effect) that is characteristic of cancer cells.
      supported_by:
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: GO; GO:0019661; P:glucose catabolic process to 
            lactate via pyruvate; IEA:Ensembl ... GO; GO:1904024; P:negative 
            regulation of glucose catabolic process to lactate via pyruvate; 
            IEA:Ensembl
  - term:
      id: GO:1990144
      label: intrinsic apoptotic signaling pathway in response to hypoxia
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 mediates apoptosis in response to severe hypoxia. Part of its
        stress response repertoire.
      action: ACCEPT
      reason: Core stress response function - p53 induces apoptosis under severe
        hypoxic stress through HIF interactions and target genes.
      supported_by:
        - reference_id: GO:0002931
          supporting_text: response to ischemia
  - term:
      id: GO:2000269
      label: regulation of fibroblast apoptotic process
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: Cell type-specific apoptotic regulation in fibroblasts.
      action: KEEP_AS_NON_CORE
      reason: Tissue-specific apoptotic function. The general apoptotic function
        is core, but fibroblast-specific regulation is context-dependent.
  - term:
      id: GO:2000378
      label: negative regulation of reactive oxygen species metabolic process
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 regulates cellular redox balance and can suppress ROS through
        antioxidant gene expression.
      action: ACCEPT
      reason: Important function - p53 maintains redox homeostasis by inducing 
        antioxidant genes like GPX1, SOD2, and others.
      supported_by:
        - reference_id: GO:0072593
          supporting_text: reactive oxygen species metabolic process
  - term:
      id: GO:2000647
      label: negative regulation of stem cell proliferation
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 maintains stem cell genomic stability by limiting 
        proliferation. Important for preventing cancer stem cells.
      action: ACCEPT
      reason: Important function - p53 restricts stem cell self-renewal to 
        maintain genomic integrity and prevent transformation.
      supported_by:
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: induces growth arrest or apoptosis depending on the 
            physiological circumstances and cell type ... Negatively regulates 
            cell division by controlling expression of a set of genes required 
            for this process
  - term:
      id: GO:2000772
      label: regulation of cellular senescence
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: Senescence induction is a core p53 tumor suppressor mechanism, 
        alternative to apoptosis for preventing damaged cell proliferation.
      action: ACCEPT
      reason: Core function - p53 induces senescence through p21 and other 
        targets as an irreversible cell cycle arrest mechanism.
      supported_by:
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: induces cell cycle arrest, DNA repair or apoptosis 
            upon binding to its target DNA sequence ... Negatively regulates 
            cell division
  - term:
      id: GO:0160020
      label: positive regulation of ferroptosis
    evidence_type: TAS
    original_reference_id: file:human/TP53/TP53-deep-research.md
    review:
      summary: p53 promotes ferroptosis through multiple mechanisms including 
        repression of SLC7A11 (cystine/glutamate antiporter), activation of 
        ALOX12, and promotion of lipid peroxidation. This is an important 
        non-apoptotic cell death mechanism.
      action: NEW
      reason: Ferroptosis regulation is a well-established p53 function 
        documented in recent literature but missing from current GO annotations.
        p53 acts as a positive regulator of ferroptosis.
      supported_by:
        - reference_id: TP53-deep-research
          supporting_text: 'Pro-ferroptotic: Represses SLC7A11, activates ALOX12,
            promotes lipid peroxidation'
        - reference_id: TP53-deep-research
          supporting_text: p53 has a dual role in ferroptosis (iron-dependent 
            programmed cell death) ... p53 act as positive regulators of 
            ferroptosis by promotion of ROS production and inhibition of 
            expression of SLC7A11
  - term:
      id: GO:1902895
      label: positive regulation of miRNA transcription
    evidence_type: IMP
    original_reference_id: PMID:30089260
    review:
      summary: p53 transcriptionally regulates multiple miRNAs including miR-34 
        family.
      action: ACCEPT
      reason: p53 regulates miRNA expression as part of its stress response 
        functions.
      supported_by:
        - reference_id: PMID:30089260
          supporting_text: we only observed significant Dox dose- and 
            time-dependent increases in the expression levels of miR-103 and 
            miR-107... we found p53-dependent induction of these miRNAs upon 
            nutlin-3a treatment
        - reference_id: PMID:30089260
          supporting_text: Using our CRISPR p53KO HCT116 cell lines, we found 
            p53-dependent induction of these miRNAs upon nutlin-3a treatment
  - term:
      id: GO:0008285
      label: negative regulation of cell population proliferation
    evidence_type: ISS
    original_reference_id: PMID:30514107
    review:
      summary: p53 negatively regulates cell proliferation as a fundamental 
        tumor suppressor mechanism.
      action: ACCEPT
      reason: Core function - p53 inhibits proliferation through cell cycle 
        arrest.
      supported_by: &id011
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: Negatively regulates cell division
        - reference_id: PMID:30514107
          supporting_text: 'CELF1/p53 axis: a sustained antiproliferative signal leading
            to villus atrophy under total parenteral nutrition.'
        - reference_id: PMID:12730672
          supporting_text: Physical and functional interaction between HCV core 
            protein and the different p73 isoforms.
        - reference_id: PMID:22783376
          supporting_text: Induction of apoptosis by cytoplasmically localized 
            wild-type p53 and the S121F mutant super p53.
  - term:
      id: GO:0051726
      label: regulation of cell cycle
    evidence_type: ISS
    original_reference_id: PMID:30514107
    review:
      summary: p53 is a master regulator of the cell cycle, inducing arrest at 
        G1/S and G2/M checkpoints.
      action: ACCEPT
      reason: Core function - cell cycle regulation is fundamental to p53's 
        tumor suppressor activity.
      supported_by: *id001
  - term:
      id: GO:1902749
      label: regulation of cell cycle G2/M phase transition
    evidence_type: IMP
    original_reference_id: PMID:10962037
    review:
      summary: p53 regulates the G2/M checkpoint, preventing mitosis of damaged 
        cells. Core cell cycle control function.
      action: ACCEPT
      reason: Core cell cycle checkpoint function with experimental evidence. 
        p53 induces G2 arrest through multiple mechanisms.
      supported_by:
        - reference_id: PMID:10962037
          supporting_text: Overexpression of MYC causes p53-dependent G2 arrest 
            of normal fibroblasts
  - term:
      id: GO:0045944
      label: positive regulation of transcription by RNA polymerase II
    evidence_type: IGI
    original_reference_id: PMID:16061649
    review:
      summary: p53 is a sequence-specific transcriptional activator that 
        upregulates target genes including p21, PUMA, BAX.
      action: ACCEPT
      reason: Fundamental molecular function - p53 activates transcription of 
        numerous target genes through binding to p53 response elements.
      supported_by: *id002
  - term:
      id: GO:0000976
      label: transcription cis-regulatory region binding
    evidence_type: IDA
    original_reference_id: PMID:15710329
    review:
      summary: Direct experimental evidence for p53 DNA binding activity. Core 
        molecular function with IDA support.
      action: ACCEPT
      reason: Core molecular function with direct experimental evidence of p53 
        binding to cis-regulatory regions.
      supported_by:
        - reference_id: PMID:15710329
          supporting_text: Human MUC1 oncoprotein regulates p53-responsive gene 
            transcription in the genotoxic stress response
  - term:
      id: GO:0042771
      label: intrinsic apoptotic signaling pathway in response to DNA damage by 
        p53 class mediator
    evidence_type: IDA
    original_reference_id: PMID:14654789
    review:
      summary: p53 is THE p53 class mediator that induces intrinsic apoptosis in
        response to DNA damage through BAX, PUMA, NOXA activation.
      action: ACCEPT
      reason: Absolutely core function - this term specifically describes p53's 
        role as the central mediator of DNA damage-induced apoptosis.
      supported_by: &id004
        - reference_id: TP53-deep-research
          supporting_text: 'Transcriptional: Activation of pro-apoptotic genes (PUMA,
            BAX, NOXA, FAS)'
        - reference_id: PMID:14654789
          supporting_text: A member of the Pyrin family, IFI16, is a novel 
            BRCA1-associated protein involved in the p53-mediated apoptosis 
            pathway.
        - reference_id: PMID:14744935
          supporting_text: Endoplasmic reticulum stress induces p53 cytoplasmic 
            localization and prevents p53-dependent apoptosis by a pathway 
            involving glycogen synthase kinase-3beta.
        - reference_id: PMID:16213212
          supporting_text: Regulation of p53 translation and induction after DNA
            damage by ribosomal protein L26 and nucleolin.
        - reference_id: PMID:17403783
          supporting_text: HLA-B-associated transcript 3 (Bat3)/Scythe is 
            essential for p300-mediated acetylation of p53.
  - term:
      id: GO:0006974
      label: DNA damage response
    evidence_type: IDA
    original_reference_id: PMID:14744935
    review:
      summary: p53 is central to the DNA damage response, being activated by and
        coordinating responses to DNA damage.
      action: ACCEPT
      reason: Core function - p53 is the 'guardian of the genome' that 
        coordinates DNA damage responses.
      supported_by: *id003
  - term:
      id: GO:0006983
      label: ER overload response
    evidence_type: IDA
    original_reference_id: PMID:14744935
    review:
      summary: p53 responds to ER stress. The cited paper shows ER stress 
        affects p53 localization and function.
      action: ACCEPT
      reason: p53 responds to ER stress as part of its cellular stress response 
        repertoire. Supported by experimental evidence.
      supported_by:
        - reference_id: PMID:14744935
          supporting_text: endoplasmic reticulum (ER) stress inhibits 
            p53-mediated apoptosis... ER stress induces GSK-3beta binding to p53
  - term:
      id: GO:0042771
      label: intrinsic apoptotic signaling pathway in response to DNA damage by 
        p53 class mediator
    evidence_type: IDA
    original_reference_id: PMID:14744935
    review:
      summary: p53 is THE p53 class mediator that induces intrinsic apoptosis in
        response to DNA damage through BAX, PUMA, NOXA activation.
      action: ACCEPT
      reason: Absolutely core function - this term specifically describes p53's 
        role as the central mediator of DNA damage-induced apoptosis.
      supported_by: *id004
  - term:
      id: GO:0042981
      label: regulation of apoptotic process
    evidence_type: IDA
    original_reference_id: PMID:14744935
    review:
      summary: p53 is a master regulator of apoptosis, both promoting and (in 
        some contexts) inhibiting apoptotic processes.
      action: ACCEPT
      reason: Core function - p53 regulates apoptosis through multiple 
        mechanisms including transcriptional activation of pro-apoptotic genes.
      supported_by: &id005
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: Apoptosis induction seems to be mediated either by 
            stimulation of BAX and FAS antigen expression
        - reference_id: PMID:14744935
          supporting_text: Endoplasmic reticulum stress induces p53 cytoplasmic 
            localization and prevents p53-dependent apoptosis by a pathway 
            involving glycogen synthase kinase-3beta.
  - term:
      id: GO:0016604
      label: nuclear body
    evidence_type: IDA
    original_reference_id: PMID:10360174
    review:
      summary: p53 localizes to nuclear bodies including PML bodies and nucleoli
        under certain conditions. This is related to its regulatory functions.
      action: ACCEPT
      reason: p53 localizes to nuclear bodies like PML bodies, especially during
        stress responses and senescence. This subcellular localization is 
        functionally relevant.
      supported_by:
        - reference_id: PMID:10360174
          supporting_text: ARF exon 2 disrupt its nucleolar localization and 
            impair its ability to block nuclear export of MDM2 and p53
  - term:
      id: GO:0000976
      label: transcription cis-regulatory region binding
    evidence_type: IDA
    original_reference_id: PMID:17996705
    review:
      summary: Additional IDA evidence for p53 DNA binding. Duplicate annotation
        with different experimental support.
      action: ACCEPT
      reason: Core molecular function with direct experimental evidence. p53 
        binds to consensus sequences in target gene promoters.
      supported_by:
        - reference_id: PMID:17996705
          supporting_text: An acetylation switch in p53 mediates holo-TFIID 
            recruitment
  - term:
      id: GO:0045944
      label: positive regulation of transcription by RNA polymerase II
    evidence_type: IDA
    original_reference_id: PMID:17599062
    review:
      summary: p53 is a sequence-specific transcriptional activator that 
        upregulates target genes including p21, PUMA, BAX.
      action: ACCEPT
      reason: Fundamental molecular function - p53 activates transcription of 
        numerous target genes through binding to p53 response elements.
      supported_by: *id002
  - term:
      id: GO:0042149
      label: cellular response to glucose starvation
    evidence_type: IDA
    original_reference_id: PMID:14744935
    review:
      summary: p53 responds to glucose starvation as part of metabolic stress 
        response.
      action: ACCEPT
      reason: p53 responds to metabolic stress including glucose deprivation.
      supported_by:
        - reference_id: PMID:14744935
          supporting_text: The tumor suppressor p53, a sensor of multiple forms 
            of cellular stress, is regulated by post-translational mechanisms to
            induce cell-cycle arrest, senescence, or apoptosis
  - term:
      id: GO:0001666
      label: response to hypoxia
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 is activated by hypoxic stress and mediates cellular 
        responses to low oxygen through HIF pathway interactions.
      action: ACCEPT
      reason: Core stress response function. Hypoxia is a well-established 
        p53-activating stress that leads to cell cycle arrest or apoptosis 
        depending on severity.
      supported_by:
        - reference_id: TP53-deep-research
          supporting_text: p53 coordinates cellular responses to diverse stress 
            signals including DNA damage, oxidative stress, hypoxia, and 
            metabolic stress
  - term:
      id: GO:0000785
      label: chromatin
    evidence_type: IBA
    original_reference_id: GO_REF:0000033
    review:
      summary: p53 associates with chromatin at target gene promoters to 
        regulate transcription.
      action: ACCEPT
      reason: Core molecular function - p53 binds to chromatin at p53 response 
        elements to regulate target gene expression.
      supported_by: &id014
        - reference_id: PDB:3TS8
          supporting_text: Crystal structure of a multidomain human p53 tetramer
            bound to the natural CDKN1A(p21) p53-response element
        - reference_id: PMID:24289924
          supporting_text: Nov 27. Phosphorylation of p53 by TAF1 inactivates 
            p53-dependent transcription in the DNA damage response.
        - reference_id: PMID:30089260
          supporting_text: p53 Regulates the Expression of LRP1 and Apoptosis 
            through a Stress Intensity-Dependent MicroRNA Feedback Loop.
        - reference_id: PMID:23629966
          supporting_text: Deacetylation of p53 induces autophagy by suppressing
            Bmf expression.
        - reference_id: PMID:22521434
          supporting_text: Hepatocyte growth factor (HGF) and 
            1,25-dihydroxyvitamin D together stimulate human bone marrow-derived
            stem cells toward the osteogenic phenotype by HGF-induced 
            up-regulation of VDR.
        - reference_id: PMID:15710329
          supporting_text: Human MUC1 oncoprotein regulates p53-responsive gene 
            transcription in the genotoxic stress response.
        - reference_id: PMID:17805299
          supporting_text: p53 is regulated by the lysine demethylase LSD1.
  - term:
      id: GO:0005634
      label: nucleus
    evidence_type: IBA
    original_reference_id: GO_REF:0000033
    review:
      summary: p53 is primarily a nuclear transcription factor, with nuclear 
        localization essential for its function.
      action: ACCEPT
      reason: Core cellular component - p53's nuclear localization is essential 
        for its transcription factor activity. Contains nuclear localization 
        signals at residues 305-322 and 369-375.
      supported_by: &id006
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: 'SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Endoplasmic
            reticulum.'
        - reference_id: PMID:14963330
          supporting_text: Direct activation of Bax by p53 mediates 
            mitochondrial membrane permeabilization and apoptosis.
        - reference_id: PMID:16322561
          supporting_text: NIR is a novel INHAT repressor that modulates the 
            transcriptional activity of p53.
        - reference_id: PMID:36108750
          supporting_text: Phase separation of p53 induced by its unstructured 
            basic region and prevented by oncogenic mutations in tetramerization
            domain.
        - reference_id: PMID:24625977
          supporting_text: Ubiquitin-like (UBX)-domain-containing protein, 
            UBXN2A, promotes cell death by interfering with the p53-Mortalin 
            interactions in colon cancer cells.
        - reference_id: PMID:26634371
          supporting_text: Epub 2015 Dec 4. Structural studies of UBXN2A and 
            mortalin interaction and the putative role of silenced UBXN2A in 
            preventing response to chemotherapy.
        - reference_id: PMID:21597459
          supporting_text: 2011 May 20. E3 ubiquitin ligase Hades negatively 
            regulates the exonuclear function of p53.
        - reference_id: PMID:19011621
          supporting_text: Arginine methylation regulates the p53 response.
        - reference_id: PMID:16479015
          supporting_text: Interferon-inducible protein IFIXalpha1 functions as 
            a negative regulator of HDM2.
        - reference_id: PMID:24289924
          supporting_text: Nov 27. Phosphorylation of p53 by TAF1 inactivates 
            p53-dependent transcription in the DNA damage response.
        - reference_id: PMID:24101517
          supporting_text: Mitochondrial disulfide relay mediates translocation 
            of p53 and partitions its subcellular activity.
        - reference_id: PMID:20810912
          supporting_text: Hypoxia downregulates p53 but induces apoptosis and 
            enhances expression of BAD in cultures of human 
            syncytiotrophoblasts.
        - reference_id: PMID:19234109
          supporting_text: Induction of SOX4 by DNA damage is critical for p53 
            stabilization and function.
        - reference_id: PMID:14744935
          supporting_text: Endoplasmic reticulum stress induces p53 cytoplasmic 
            localization and prevents p53-dependent apoptosis by a pathway 
            involving glycogen synthase kinase-3beta.
        - reference_id: PMID:17403783
          supporting_text: HLA-B-associated transcript 3 (Bat3)/Scythe is 
            essential for p300-mediated acetylation of p53.
        - reference_id: PMID:20096447
          supporting_text: USP10 regulates p53 localization and stability by 
            deubiquitinating p53.
        - reference_id: PMID:18756595
          supporting_text: Prognostic significance of BMP and activin 
            membrane-bound inhibitor in colorectal cancer.
        - reference_id: PMID:16507995
          supporting_text: Myosin VI is a mediator of the p53-dependent cell 
            survival pathway.
        - reference_id: PMID:7720704
          supporting_text: Direct involvement of p53 in programmed cell death of
            oligodendrocytes.
  - term:
      id: GO:0042771
      label: intrinsic apoptotic signaling pathway in response to DNA damage by 
        p53 class mediator
    evidence_type: IBA
    original_reference_id: GO_REF:0000033
    review:
      summary: p53 is THE p53 class mediator that induces intrinsic apoptosis in
        response to DNA damage through BAX, PUMA, NOXA activation.
      action: ACCEPT
      reason: Absolutely core function - this term specifically describes p53's 
        role as the central mediator of DNA damage-induced apoptosis.
      supported_by: *id004
  - term:
      id: GO:0042981
      label: regulation of apoptotic process
    evidence_type: IBA
    original_reference_id: GO_REF:0000033
    review:
      summary: p53 is a master regulator of apoptosis, both promoting and (in 
        some contexts) inhibiting apoptotic processes.
      action: ACCEPT
      reason: Core function - p53 regulates apoptosis through multiple 
        mechanisms including transcriptional activation of pro-apoptotic genes.
      supported_by: *id005
  - term:
      id: GO:0045944
      label: positive regulation of transcription by RNA polymerase II
    evidence_type: IBA
    original_reference_id: GO_REF:0000033
    review:
      summary: p53 is a sequence-specific transcriptional activator that 
        upregulates target genes including p21, PUMA, BAX.
      action: ACCEPT
      reason: Fundamental molecular function - p53 activates transcription of 
        numerous target genes through binding to p53 response elements.
      supported_by: *id002
  - term:
      id: GO:1990841
      label: promoter-specific chromatin binding
    evidence_type: IBA
    original_reference_id: GO_REF:0000033
    review:
      summary: p53 binds to promoter-specific chromatin regions containing p53 
        response elements.
      action: ACCEPT
      reason: Core molecular function - p53 specifically binds to promoter 
        regions of target genes through sequence-specific DNA binding.
      supported_by: &id013
        - reference_id: PDB:3TS8
          supporting_text: Crystal structure shows p53 binding to CDKN1A(p21) 
            promoter response element
        - reference_id: PMID:24652652
          supporting_text: 2014 Mar 20. DRAGO (KIAA0247), a new DNA 
            damage-responsive, p53-inducible gene that cooperates with p53 as 
            oncosuppressor.
        - reference_id: PMID:20725088
          supporting_text: 2010 Aug 20. Primate-specific RFPL1 gene controls 
            cell-cycle progression through cyclin B1/Cdc2 degradation.
        - reference_id: PMID:24356969
          supporting_text: 2013 Dec 19. Rbm24, an RNA-binding protein and a 
            target of p53, regulates p21 expression via mRNA stability.
  - term:
      id: GO:0000981
      label: DNA-binding transcription factor activity, RNA polymerase 
        II-specific
    evidence_type: IBA
    original_reference_id: GO_REF:0000033
    review:
      summary: p53 is a DNA-binding transcription factor that regulates RNA 
        polymerase II transcription of target genes.
      action: ACCEPT
      reason: Core molecular function - p53 is a well-characterized 
        sequence-specific transcription factor for RNA pol II.
      supported_by: &id019
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: Multifunctional transcription factor
        - reference_id: PMID:34381247
          supporting_text: Epub 2021 Aug 11. The tumor suppressor folliculin 
            inhibits lactate dehydrogenase A and regulates the Warburg effect.
        - reference_id: PMID:35618207
          supporting_text: Ser392 phosphorylation modulated a switch between p53
            and transcriptional condensates.
        - reference_id: PMID:36634798
          supporting_text: Phosphorylation and specific DNA improved the 
            incorporation ability of p53 into functional condensates.
        - reference_id: PMID:38653238
          supporting_text: 2024 Apr 22. Alanyl-tRNA synthetase, AARS1, is a 
            lactate sensor and lactyltransferase that lactylates p53 and 
            contributes to tumorigenesis.
        - reference_id: PMID:24289924
          supporting_text: Nov 27. Phosphorylation of p53 by TAF1 inactivates 
            p53-dependent transcription in the DNA damage response.
        - reference_id: PMID:17310983
          supporting_text: 'Feb 19. Ribosomal protein S7 as a novel modulator of p53-MDM2
            interaction: binding to MDM2, stabilization of p53 protein, and activation
            of p53 function.'
        - reference_id: PMID:24652652
          supporting_text: 2014 Mar 20. DRAGO (KIAA0247), a new DNA 
            damage-responsive, p53-inducible gene that cooperates with p53 as 
            oncosuppressor.
  - term:
      id: GO:0000978
      label: RNA polymerase II cis-regulatory region sequence-specific DNA 
        binding
    evidence_type: IBA
    original_reference_id: GO_REF:0000033
    review:
      summary: p53 binds sequence-specifically to p53 response elements in 
        cis-regulatory regions of target genes.
      action: ACCEPT
      reason: Core molecular function - p53's DNA-binding domain binds to 
        consensus p53 response elements (p53REs) with high specificity.
      supported_by: &id010
        - reference_id: TP53-deep-research
          supporting_text: TP53 is fundamentally a transcription factor that 
            binds sequence-specifically to p53 response elements (p53REs) in 
            target gene promoters
        - reference_id: PMID:24289924
          supporting_text: Nov 27. Phosphorylation of p53 by TAF1 inactivates 
            p53-dependent transcription in the DNA damage response.
        - reference_id: PMID:22578566
          supporting_text: Apr 19. Oncosuppressive role of p53-induced miR-205 
            in triple negative breast cancer.
  - term:
      id: GO:0003677
      label: DNA binding
    evidence_type: IEA
    original_reference_id: GO_REF:0000120
    review:
      summary: p53 binds DNA through its central DNA-binding domain (residues 
        102-292) to p53 response elements.
      action: ACCEPT
      reason: Core molecular function - DNA binding is essential for p53's 
        transcription factor activity.
      supported_by: &id032
        - reference_id: TP53-deep-research
          supporting_text: 'DNA-Binding Domain (DBD): Location: Residues 102-292;
            Structure: beta-sandwich core with DNA-binding loops'
        - reference_id: PMID:15358771
          supporting_text: 2004 Sep 9. Hsp90 regulates the activity of wild type
            p53 under physiological and elevated temperatures.
        - reference_id: PMID:15629713
          supporting_text: Binding of natively unfolded HIF-1alpha ODD domain to
            p53.
        - reference_id: PMID:2144364
          supporting_text: Transcriptional activation by wild-type but not 
            transforming mutants of the p53 anti-oncogene.
  - term:
      id: GO:0003700
      label: DNA-binding transcription factor activity
    evidence_type: IEA
    original_reference_id: GO_REF:0000120
    review:
      summary: p53 is a well-characterized DNA-binding transcription factor that
        activates and represses target genes.
      action: ACCEPT
      reason: Core molecular function - p53 is one of the best-characterized 
        transcription factors, regulating >500 target genes.
      supported_by: &id015
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: Multifunctional transcription factor
        - reference_id: PMID:16479015
          supporting_text: Interferon-inducible protein IFIXalpha1 functions as 
            a negative regulator of HDM2.
        - reference_id: PMID:26334721
          supporting_text: 2015 Sep 3. Jmjd5 functions as a regulator of p53 
            signaling during mouse embryogenesis.
        - reference_id: PMID:18549481
          supporting_text: TATA binding protein associated factor 3 (TAF3) 
            interacts with p53 and inhibits its function.
        - reference_id: PMID:7587074
          supporting_text: 'Targets for transcriptional activation by wild-type p53:
            endogenous retroviral LTR, immunoglobulin-like promoter, and an internal
            promoter of the mdm2 gene.'
  - term:
      id: GO:0005634
      label: nucleus
    evidence_type: IEA
    original_reference_id: GO_REF:0000120
    review:
      summary: p53 is primarily a nuclear transcription factor, with nuclear 
        localization essential for its function.
      action: ACCEPT
      reason: Core cellular component - p53's nuclear localization is essential 
        for its transcription factor activity. Contains nuclear localization 
        signals at residues 305-322 and 369-375.
      supported_by: *id006
  - term:
      id: GO:0005737
      label: cytoplasm
    evidence_type: IEA
    original_reference_id: GO_REF:0000120
    review:
      summary: p53 localizes to cytoplasm as well as nucleus, with cytoplasmic 
        localization important for regulation.
      action: ACCEPT
      reason: Core cellular component - p53 shuttles between nucleus and 
        cytoplasm, with cytoplasmic localization important for MDM2-mediated 
        regulation.
      supported_by: &id020
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: 'SUBCELLULAR LOCATION: Nucleus. Cytoplasm.'
        - reference_id: PMID:24625977
          supporting_text: Ubiquitin-like (UBX)-domain-containing protein, 
            UBXN2A, promotes cell death by interfering with the p53-Mortalin 
            interactions in colon cancer cells.
        - reference_id: PMID:26634371
          supporting_text: Epub 2015 Dec 4. Structural studies of UBXN2A and 
            mortalin interaction and the putative role of silenced UBXN2A in 
            preventing response to chemotherapy.
        - reference_id: PMID:21597459
          supporting_text: 2011 May 20. E3 ubiquitin ligase Hades negatively 
            regulates the exonuclear function of p53.
        - reference_id: PMID:19011621
          supporting_text: Arginine methylation regulates the p53 response.
        - reference_id: PMID:16479015
          supporting_text: Interferon-inducible protein IFIXalpha1 functions as 
            a negative regulator of HDM2.
        - reference_id: PMID:23629966
          supporting_text: Deacetylation of p53 induces autophagy by suppressing
            Bmf expression.
        - reference_id: PMID:20810912
          supporting_text: Hypoxia downregulates p53 but induces apoptosis and 
            enhances expression of BAD in cultures of human 
            syncytiotrophoblasts.
        - reference_id: PMID:16131611
          supporting_text: p53 isoforms can regulate p53 transcriptional 
            activity.
        - reference_id: PMID:14744935
          supporting_text: Endoplasmic reticulum stress induces p53 cytoplasmic 
            localization and prevents p53-dependent apoptosis by a pathway 
            involving glycogen synthase kinase-3beta.
        - reference_id: PMID:20096447
          supporting_text: USP10 regulates p53 localization and stability by 
            deubiquitinating p53.
        - reference_id: PMID:7720704
          supporting_text: Direct involvement of p53 in programmed cell death of
            oligodendrocytes.
  - term:
      id: GO:0005759
      label: mitochondrial matrix
    evidence_type: IEA
    original_reference_id: GO_REF:0000120
    review:
      summary: p53 localizes to mitochondria where it can directly promote 
        apoptosis through non-transcriptional mechanisms.
      action: ACCEPT
      reason: Important cellular localization - p53 can translocate to 
        mitochondria and directly trigger apoptosis through BAX/BAK activation.
      supported_by:
        - reference_id: TP53-deep-research
          supporting_text: 'Non-transcriptional: Direct mitochondrial translocation
            and interaction with BCL-2 family proteins'
  - term:
      id: GO:0005783
      label: endoplasmic reticulum
    evidence_type: IEA
    original_reference_id: GO_REF:0000044
    review:
      summary: p53 localizes to endoplasmic reticulum where it responds to ER 
        stress.
      action: ACCEPT
      reason: p53 responds to ER stress signals and can localize to ER during 
        stress responses.
      supported_by:
        - reference_id: PMID:14744935
          supporting_text: Endoplasmic reticulum stress induces p53 cytoplasmic 
            localization
  - term:
      id: GO:0005813
      label: centrosome
    evidence_type: IEA
    original_reference_id: GO_REF:0000044
    review:
      summary: p53 has been reported to localize to centrosomes but this is not 
        a primary localization.
      action: KEEP_AS_NON_CORE
      reason: Non-core localization. p53 may associate with centrosomes in 
        certain contexts but this is not a primary function.
  - term:
      id: GO:0006355
      label: regulation of DNA-templated transcription
    evidence_type: IEA
    original_reference_id: GO_REF:0000120
    review:
      summary: p53 regulates DNA-templated transcription as both an activator 
        and repressor.
      action: ACCEPT
      reason: Core molecular function - transcription regulation is fundamental 
        to p53's tumor suppressor activity.
      supported_by: &id036
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: Multifunctional transcription factor
        - reference_id: PMID:25417702
          supporting_text: Functional interplay between MDM2, p63/p73 and mutant
            p53.
        - reference_id: PMID:7587074
          supporting_text: 'Targets for transcriptional activation by wild-type p53:
            endogenous retroviral LTR, immunoglobulin-like promoter, and an internal
            promoter of the mdm2 gene.'
  - term:
      id: GO:0006915
      label: apoptotic process
    evidence_type: IEA
    original_reference_id: GO_REF:0000120
    review:
      summary: p53 is a master regulator of apoptosis, inducing cell death 
        through multiple pathways.
      action: ACCEPT
      reason: Core function - apoptosis induction is one of p53's primary tumor 
        suppressor mechanisms.
      supported_by:
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: induces growth arrest or apoptosis depending on the 
            physiological circumstances
  - term:
      id: GO:0010212
      label: response to ionizing radiation
    evidence_type: IEA
    original_reference_id: GO_REF:0000117
    review:
      summary: p53 is strongly activated by ionizing radiation-induced DNA 
        damage.
      action: ACCEPT
      reason: Core function - ionizing radiation is a classic p53-activating 
        stress through ATM/ATR signaling.
      supported_by:
        - reference_id: TP53-deep-research
          supporting_text: p53 serves as a central node in the DNA damage 
            response network
  - term:
      id: GO:0012501
      label: programmed cell death
    evidence_type: IEA
    original_reference_id: GO_REF:0000043
    review:
      summary: p53 induces programmed cell death through apoptosis, and also 
        regulates other cell death pathways.
      action: ACCEPT
      reason: Core function - p53 is a master regulator of programmed cell 
        death.
      supported_by:
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: induces growth arrest or apoptosis
  - term:
      id: GO:0016605
      label: PML body
    evidence_type: IEA
    original_reference_id: GO_REF:0000044
    review:
      summary: p53 localizes to PML nuclear bodies, which is important for its 
        post-translational modification and activation.
      action: ACCEPT
      reason: Important regulatory localization - p53 accumulates in PML bodies 
        during stress responses where it undergoes acetylation.
      supported_by: &id041
        - reference_id: TP53-deep-research
          supporting_text: p53 localizes to nuclear bodies like PML bodies, 
            especially during stress responses
        - reference_id: PMID:22869143
          supporting_text: BMK1 is involved in the regulation of p53 through 
            disrupting the PML-MDM2 interaction.
        - reference_id: PMID:12006491
          supporting_text: Human SIR2 deacetylates p53 and antagonizes 
            PML/p53-induced cellular senescence.
  - term:
      id: GO:0046872
      label: metal ion binding
    evidence_type: IEA
    original_reference_id: GO_REF:0000043
    review:
      summary: p53's DNA-binding domain contains a zinc coordination site 
        essential for structural stability.
      action: ACCEPT
      reason: Core structural feature - the p53 DNA-binding domain contains a 
        zinc atom coordinated by Cys176, His179, Cys238, and Cys242.
      supported_by:
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: Contains zinc coordination site in DNA-binding domain
  - term:
      id: GO:0048511
      label: rhythmic process
    evidence_type: IEA
    original_reference_id: GO_REF:0000043
    review:
      summary: p53 may influence circadian rhythms but this is not a core 
        function.
      action: KEEP_AS_NON_CORE
      reason: Non-core function. p53 has connections to circadian biology but 
        this is not its primary role.
  - term:
      id: GO:0051053
      label: negative regulation of DNA metabolic process
    evidence_type: IEA
    original_reference_id: GO_REF:0000117
    review:
      summary: p53 negatively regulates DNA replication as part of cell cycle 
        arrest response.
      action: ACCEPT
      reason: Core function related to cell cycle control - p53 inhibits DNA 
        replication in damaged cells.
      supported_by:
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: Negatively regulates cell division
  - term:
      id: GO:0051262
      label: protein tetramerization
    evidence_type: IEA
    original_reference_id: GO_REF:0000002
    review:
      summary: p53 forms tetramers through its oligomerization domain (residues 
        325-356), essential for DNA binding.
      action: ACCEPT
      reason: Core structural feature - tetramerization is essential for 
        high-affinity DNA binding and p53 function.
      supported_by:
        - reference_id: PDB:1C26
          supporting_text: Crystal structure of p53 tetramerization domain shows
            dimer-of-dimers architecture
  - term:
      id: GO:0071456
      label: cellular response to hypoxia
    evidence_type: IEA
    original_reference_id: GO_REF:0000117
    review:
      summary: p53 mediates cellular responses to hypoxia, including cell cycle 
        arrest and apoptosis induction.
      action: ACCEPT
      reason: Core stress response function - p53 responds to hypoxic stress and
        coordinates appropriate cellular responses.
      supported_by: &id039
        - reference_id: GO:1990144
          supporting_text: intrinsic apoptotic signaling pathway in response to 
            hypoxia
        - reference_id: PMID:20810912
          supporting_text: Hypoxia downregulates p53 but induces apoptosis and 
            enhances expression of BAD in cultures of human 
            syncytiotrophoblasts.
  - term:
      id: GO:0097190
      label: apoptotic signaling pathway
    evidence_type: IEA
    original_reference_id: GO_REF:0000117
    review:
      summary: p53 is a master regulator of apoptotic signaling pathways.
      action: ACCEPT
      reason: Core function - p53 activates both intrinsic and extrinsic 
        apoptotic signaling.
      supported_by:
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: Apoptosis induction seems to be mediated either by 
            stimulation of BAX and FAS antigen expression
  - term:
      id: GO:2001242
      label: regulation of intrinsic apoptotic signaling pathway
    evidence_type: IEA
    original_reference_id: GO_REF:0000117
    review:
      summary: p53 both executes and regulates the intrinsic apoptotic signaling
        pathway.
      action: ACCEPT
      reason: Core function - p53 is a key regulator of intrinsic apoptotic 
        signaling through multiple target genes.
      supported_by:
        - reference_id: TP53-deep-research
          supporting_text: p53 triggers apoptosis through both 
            transcription-dependent and transcription-independent mechanisms
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:10196247
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:10196247
          supporting_text: Characterization of an E1A-CBP interaction defines a 
            novel transcriptional adapter motif (TRAM) in CBP/p300.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:10226625
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:10226625
          supporting_text: The yeast two-hybrid system reveals no interaction 
            between p73 alpha and SV40 large T-antigen.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:10518217
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:10518217
          supporting_text: A novel cofactor for p300 that regulates the p53 
            response.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:10823891
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:10823891
          supporting_text: The Huntington's disease protein interacts with p53 
            and CREB-binding protein and represses transcription.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:11146555
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:11146555
          supporting_text: Functional interaction between p53 and the 
            interferon-inducible nucleoprotein IFI 16.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:11178989
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:11178989
          supporting_text: MDM2 enhances the function of estrogen receptor alpha
            in human breast cancer cells.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:11359905
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:11359905
          supporting_text: The corepressor mSin3a interacts with the 
            proline-rich domain of p53 and protects p53 from proteasome-mediated
            degradation.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:11388671
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:11388671
          supporting_text: A putative protein inhibitor of activated STAT 
            (PIASy) interacts with p53 and inhibits p53-mediated transactivation
            but not apoptosis.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:11427532
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:11427532
          supporting_text: 2001 Jun 26. p53 Modulates the exonuclease activity 
            of Werner syndrome protein.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:11672523
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:11672523
          supporting_text: hSIR2(SIRT1) functions as an NAD-dependent p53 
            deacetylase.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:11706030
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:11706030
          supporting_text: Nov 12. Differential effect of ik3-1/cables on p53- 
            and p73-induced cell death.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:11877378
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:11877378
          supporting_text: Structure of the 53BP1 BRCT region bound to p53 and 
            its comparison to the Brca1 BRCT structure.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:12006491
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:12006491
          supporting_text: Human SIR2 deacetylates p53 and antagonizes 
            PML/p53-induced cellular senescence.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:12080348
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:12080348
          supporting_text: Nucleophosmin regulates the stability and 
            transcriptional activity of p53.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:12620801
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:12620801
          supporting_text: Requirement of E6AP and the features of human 
            papillomavirus E6 necessary to support degradation of p53.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:12667443
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:12667443
          supporting_text: p53 has a direct apoptogenic role at the 
            mitochondria.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:12692135
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:12692135
          supporting_text: 2003 Apr 10. P63 alpha mutations lead to aberrant 
            splicing of keratinocyte growth factor receptor in the Hay-Wells 
            syndrome.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:12702766
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:12702766
          supporting_text: Characterization of cells and gene-targeted mice 
            deficient for the p53-binding kinase homeodomain-interacting protein
            kinase 1 (HIPK1).
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:12915590
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:12915590
          supporting_text: Aug 12. Cellular stress and DNA damage invoke 
            temporally distinct Mdm2, p53 and PML complexes and damage-specific 
            nuclear relocalization.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:14557665
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:14557665
          supporting_text: Adenovirus E1B 55-kilodalton oncoprotein binds to 
            Daxx and eliminates enhancement of p53-dependent transcription by 
            Daxx.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:14627987
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:14627987
          supporting_text: PARP-1 binds E2F-1 independently of its DNA binding 
            and catalytic domains, and acts as a novel coactivator of 
            E2F-1-mediated transcription during re-entry of quiescent cells into
            S phase.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:14744935
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:14744935
          supporting_text: Endoplasmic reticulum stress induces p53 cytoplasmic 
            localization and prevents p53-dependent apoptosis by a pathway 
            involving glycogen synthase kinase-3beta.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:14759370
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:14759370
          supporting_text: Structural mechanism of the bromodomain of the 
            coactivator CBP in p53 transcriptional activation.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:14963330
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:14963330
          supporting_text: Direct activation of Bax by p53 mediates 
            mitochondrial membrane permeabilization and apoptosis.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:14985081
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:14985081
          supporting_text: Hepatitis C virus core protein interacts with 
            p53-binding protein, 53BP2/Bbp/ASPP2, and inhibits p53-mediated 
            apoptosis.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:15044383
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:15044383
          supporting_text: Mar 25. Aprataxin, a novel protein that protects 
            against genotoxic stress.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:15068796
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:15068796
          supporting_text: C. elegans SGK-1 is the critical component in the 
            Akt/PKB kinase complex to control stress response and life span.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:15133049
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:15133049
          supporting_text: 2004 May 8. NMR structure of the N-terminal domain of
            SUMO ligase PIAS1 and its interaction with tumor suppressor p53 and 
            A/T-rich DNA oligomers.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:15144954
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:15144954
          supporting_text: Nucleolar protein NPM interacts with HDM2 and 
            protects tumor suppressor protein p53 from HDM2-mediated 
            degradation.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:15205477
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:15205477
          supporting_text: Jun 17. Calorie restriction promotes mammalian cell 
            survival by inducing the SIRT1 deacetylase.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:15310821
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:15310821
          supporting_text: Identification of ASK and clock-associated proteins 
            as molecular partners of LKP2 (LOV kelch protein 2) in Arabidopsis.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:15364927
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:15364927
          supporting_text: 2004 Sep 10. Negative regulation of p53 functions by 
            Daxx and the involvement of MDM2.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:15525938
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:15525938
          supporting_text: Regulation of p53 activity through lysine 
            methylation.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:15542844
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:15542844
          supporting_text: p53 Stabilization and accumulation induced by human 
            vaccinia-related kinase 1.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:15580310
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:15580310
          supporting_text: '17beta-Estradiol upregulates and activates WOX1/WWOXv1
            and WOX2/WWOXv2 in vitro: potential role in cancerous progression of breast
            and prostate to a premetastatic state in vivo.'
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:15604276
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:15604276
          supporting_text: Adenoviral E1A targets Mdm4 to stabilize tumor 
            suppressor p53.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:15660129
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:15660129
          supporting_text: 'The DEAD box protein p68: a novel transcriptional coactivator
            of the p53 tumour suppressor.'
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:15735006
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:15735006
          supporting_text: p53 modulates RPA-dependent and RPA-independent WRN 
            helicase activity.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:15782130
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:15782130
          supporting_text: BARD1 induces apoptosis by catalysing phosphorylation
            of p53 by DNA-damage response kinase.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:15855171
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:15855171
          supporting_text: 2005 Apr 25. Direct interaction of the N-terminal 
            domain of focal adhesion kinase with the N-terminal transactivation 
            domain of p53.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:15916963
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:15916963
          supporting_text: Loss of HAUSP-mediated deubiquitination contributes 
            to DNA damage-induced destabilization of Hdmx and Hdm2.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:15960975
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:15960975
          supporting_text: Physical association and coordinate function of the 
            H3 K4 methyltransferase MLL1 and the H4 K16 acetyltransferase MOF.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:15989956
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:15989956
          supporting_text: ARF-BP1/Mule is a critical mediator of the ARF tumor 
            suppressor.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:16003391
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:16003391
          supporting_text: AtBAG6, a novel calmodulin-binding protein, induces 
            programmed cell death in yeast and plants.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:16151013
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:16151013
          supporting_text: PUMA couples the nuclear and cytoplasmic proapoptotic
            function of p53.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:16169070
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:16169070
          supporting_text: 'A human protein-protein interaction network: a resource
            for annotating the proteome.'
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:16189514
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:16189514
          supporting_text: Towards a proteome-scale map of the human 
            protein-protein interaction network.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:16227626
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:16227626
          supporting_text: Oct 14. Physical interaction and mutual 
            transrepression between CCAAT/enhancer-binding protein beta and the 
            p53 tumor suppressor.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:16319068
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:16319068
          supporting_text: Nov 30. Camptothecin induces nuclear export of 
            prohibitin preferentially in transformed cells through a 
            CRM-1-dependent mechanism.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:16322561
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:16322561
          supporting_text: NIR is a novel INHAT repressor that modulates the 
            transcriptional activity of p53.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:16376338
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:16376338
          supporting_text: 2005 Dec 19. Protein kinase A phosphorylates and 
            regulates dimerization of 14-3-3 epsilon.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:16376884
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:16376884
          supporting_text: '2005 Dec 19. Characterisation of the interface between
            nucleophosmin (NPM) and p53: potential role in p53 stabilisation.'
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:16377624
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:16377624
          supporting_text: Dec 23. Protein kinase C delta regulates Ser46 
            phosphorylation of p53 tumor suppressor in the apoptotic response to
            DNA damage.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:16402859
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:16402859
          supporting_text: 'Structural basis of competitive recognition of p53 and
            MDM2 by HAUSP/USP7: implications for the regulation of the p53-MDM2 pathway.'
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:16415881
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:16415881
          supporting_text: Jan 15. Structural basis for the methylation site 
            specificity of SET7/9.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:16432196
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:16432196
          supporting_text: The central region of HDM2 provides a second binding 
            site for p53.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:16442532
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:16442532
          supporting_text: 2006 Jan 20. Interaction of metallothionein with 
            tumor suppressor p53 protein.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:16474402
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:16474402
          supporting_text: Feb 12. Molecular recognition of p53 and MDM2 by 
            USP7/HAUSP.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:16493710
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:16493710
          supporting_text: Surface plasmon resonance imaging protein arrays for 
            analysis of triple protein interactions of HPV, E6, E6AP, and p53.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:16511572
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:16511572
          supporting_text: Mar 2. 14-3-3gamma binds to MDMX that is 
            phosphorylated by UV-activated Chk1, resulting in p53 activation.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:16601686
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:16601686
          supporting_text: Tip60 and p400 are both required for UV-induced 
            apoptosis but play antagonistic roles in cell cycle progression.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:16611888
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:16611888
          supporting_text: Targeting of p300/CREB binding protein coactivators 
            by simian virus 40 is mediated through p53.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:16616141
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:16616141
          supporting_text: Epub 2006 Mar 29. Evidence that low doses of Taxol 
            enhance the functional transactivatory properties of p53 on p21 waf 
            promoter in MCF-7 breast cancer cells.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:16690937
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:16690937
          supporting_text: NS3 protein of Hepatitis C virus associates with the 
            tumour suppressor p53 and inhibits its function in an NS3 
            sequence-dependent manner.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:16713569
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:16713569
          supporting_text: A protein-protein interaction network for human 
            inherited ataxias and disorders of Purkinje cell degeneration.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:16732283
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:16732283
          supporting_text: May 28. Lysine activation and functional analysis of 
            E2-mediated conjugation in the SUMO pathway.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:16753148
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:16753148
          supporting_text: Epub 2006 May 30. Polo-like kinase 1 regulates 
            mitotic arrest after UV irradiation through dephosphorylation of p53
            and inducing p53 degradation.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:16793543
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:16793543
          supporting_text: 'Structure of the Tfb1/p53 complex: Insights into the interaction
            between the p62/Tfb1 subunit of TFIIH and the activation domain of p53.'
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:16845383
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:16845383
          supporting_text: Critical role for Daxx in regulating Mdm2.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:16847267
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:16847267
          supporting_text: MAGE-A tumor antigens target p53 transactivation 
            function through histone deacetylase recruitment and confer 
            resistance to chemotherapeutic agents.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:16951253
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:16951253
          supporting_text: 'Crystal structure of SV40 large T-antigen bound to p53:
            interplay between a viral oncoprotein and a cellular tumor suppressor.'
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:16959611
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:16959611
          supporting_text: 'Gain of function of mutant p53: the mutant p53/NF-Y protein
            complex reveals an aberrant transcriptional mechanism of cell cycle regulation.'
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:17080083
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:17080083
          supporting_text: Inactivation of the p53 pathway in retinoblastoma.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:17098746
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:17098746
          supporting_text: 2006 Nov 9. FBXO11 promotes the Neddylation of p53 
            and inhibits its transcriptional activity.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:17108971
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:17108971
          supporting_text: Repression of p53 activity by Smyd2-mediated 
            methylation.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:17139261
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:17139261
          supporting_text: Nov 30. p53 mediates the negative regulation of MDM2 
            by orphan receptor TR3.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:17159902
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:17159902
          supporting_text: An essential function of the extreme C-terminus of 
            MDM2 can be provided by MDMX.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:17170702
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:17170702
          supporting_text: Cytoplasmic destruction of p53 by the endoplasmic 
            reticulum-resident ubiquitin ligase 'Synoviolin'.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:17184779
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:17184779
          supporting_text: 2006 Dec 13. Identification of differential proteins 
            in nasopharyngeal carcinoma cells with p53 silence by proteome 
            analysis.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:17245430
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:17245430
          supporting_text: A specific PP2A regulatory subunit, B56gamma, 
            mediates DNA damage-induced dephosphorylation of p53 at Thr55.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:17254968
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:17254968
          supporting_text: PRAK is essential for ras-induced senescence and 
            tumor suppression.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:17268548
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:17268548
          supporting_text: Monoubiquitylation promotes mitochondrial p53 
            translocation.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:17274640
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:17274640
          supporting_text: A limited screen for protein interactions reveals new
            roles for protein phosphatase 1 in cell cycle control and apoptosis.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:17290220
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:17290220
          supporting_text: The deubiquitinating enzyme USP2a regulates the p53 
            pathway by targeting Mdm2.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:17298945
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:17298945
          supporting_text: 2007 Feb 12. The conserved CPH domains of Cul7 and 
            PARC are protein-protein interaction modules that bind the 
            tetramerization domain of p53.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:17310983
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:17310983
          supporting_text: 'Feb 19. Ribosomal protein S7 as a novel modulator of p53-MDM2
            interaction: binding to MDM2, stabilization of p53 protein, and activation
            of p53 function.'
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:17347673
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:17347673
          supporting_text: Stra13 is induced by genotoxic stress and regulates 
            ionizing-radiation-induced apoptosis.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:17438265
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:17438265
          supporting_text: Four domains of p300 each bind tightly to a sequence 
            spanning both transactivation subdomains of p53.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:17470788
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:17470788
          supporting_text: PACT is a negative regulator of p53 and essential for
            cell growth and embryonic development.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:17482142
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:17482142
          supporting_text: Attenuation of DNA damage checkpoint by PBK, a novel 
            mitotic kinase, involves protein-protein interaction with tumor 
            suppressor p53.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:17568776
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:17568776
          supporting_text: Jun 14. Phosphorylation of Pirh2 by 
            calmodulin-dependent kinase II impairs its ability to ubiquitinate 
            p53.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:17612295
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:17612295
          supporting_text: Yeast split-ubiquitin-based cytosolic screening 
            system to detect interactions between transcriptionally active 
            proteins.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:17662718
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:17662718
          supporting_text: Epub 2007 Jul 23. Protein-protein interactions among 
            human lens acidic and basic beta-crystallins.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:17719542
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:17719542
          supporting_text: hCAS/CSE1L associates with chromatin and regulates 
            expression of select p53 target genes.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:17805299
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:17805299
          supporting_text: p53 is regulated by the lysine demethylase LSD1.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:17875722
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:17875722
          supporting_text: Efficient p53 activation and apoptosis by 
            simultaneous disruption of binding to MDM2 and MDMX.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:17906639
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:17906639
          supporting_text: Sep 30. The prolyl isomerase Pin1 orchestrates p53 
            acetylation and dissociation from the apoptosis inhibitor iASPP.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:17964266
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:17964266
          supporting_text: Active regulator of SIRT1 cooperates with SIRT1 and 
            facilitates suppression of p53 activity.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:18087040
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:18087040
          supporting_text: Regulation of p53 tetramerization and nuclear export 
            by ARC.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:18172499
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:18172499
          supporting_text: NUMB controls p53 tumour suppressor activity.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:18230339
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:18230339
          supporting_text: Activation of p53-dependent responses in tumor cells 
            treated with a PARC-interacting peptide.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:18235501
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:18235501
          supporting_text: DBC1 is a negative regulator of SIRT1.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:18235502
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:18235502
          supporting_text: Negative regulation of the deacetylase SIRT1 by DBC1.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:18275817
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:18275817
          supporting_text: Biochemical and structural studies of ASPP proteins 
            reveal differential binding to p53, p63, and p73.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:18309296
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:18309296
          supporting_text: Proteasome activator PA28 gamma regulates p53 by 
            enhancing its MDM2-mediated degradation.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:18316739
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:18316739
          supporting_text: Temporal activation of p53 by a specific MDM2 
            inhibitor is selectively toxic to tumors and leads to complete tumor
            growth inhibition.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:18354501
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:18354501
          supporting_text: 'Structural insight into the TFIIE-TFIIH interaction: TFIIE
            and p53 share the binding region on TFIIH.'
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:18388957
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:18388957
          supporting_text: NAD(P)H quinone oxidoreductase 1 inhibits the 
            proteasomal degradation of the tumour suppressor p33(ING1b).
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:18391200
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:18391200
          supporting_text: Structure of tumor suppressor p53 and its 
            intrinsically disordered N-terminal transactivation domain.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:18485870
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:18485870
          supporting_text: Acetylation is indispensable for p53 activation.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:18504427
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:18504427
          supporting_text: Twist and p53 reciprocally regulate target genes via 
            direct interaction.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:18510931
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:18510931
          supporting_text: Ectodermal factor restricts mesoderm differentiation 
            by inhibiting p53.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:18566590
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:18566590
          supporting_text: The tumour suppressor RASSF1A promotes MDM2 
            self-ubiquitination by disrupting the MDM2-DAXX-HAUSP complex.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:18624398
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:18624398
          supporting_text: Protein interaction data set highlighted with human 
            Ras-MAPK/PI3K signaling pathways.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:18656471
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:18656471
          supporting_text: Epub 2008 Jul 24. Microtubule-associated protein 1B 
            light chain (MAP1B-LC1) negatively regulates the activity of tumor 
            suppressor p53 in neuroblastoma cells.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:18695251
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:18695251
          supporting_text: AIMP2/p38, the scaffold for the multi-tRNA synthetase
            complex, responds to genotoxic stresses via p53.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:18812399
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:18812399
          supporting_text: Sep 23. 14-3-3 activation of DNA binding of p53 by 
            enhancing its association into tetramers.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:18952844
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:18952844
          supporting_text: Inhibition of Thr-55 phosphorylation restores p53 
            nuclear localization and sensitizes cancer cells to DNA damage.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:18977328
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:18977328
          supporting_text: Inactivation of the CYLD deubiquitinase by HPV E6 
            mediates hypoxia-induced NF-kappaB activation.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:19008854
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:19008854
          supporting_text: Interferon-inducible protein, P56, inhibits HPV DNA 
            replication by binding to the viral protein E1.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:19043414
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:19043414
          supporting_text: 2008 Nov 30. Molecular basis of Pirh2-mediated p53 
            ubiquitylation.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:19098711
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:19098711
          supporting_text: RYBP stabilizes p53 by modulating MDM2.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:19166840
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:19166840
          supporting_text: 2009 Jan 21. FKBP25, a novel regulator of the p53 
            pathway, induces the degradation of MDM2 and activation of p53.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:19196987
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:19196987
          supporting_text: Phosphorylation of p53 by IkappaB kinase 2 promotes 
            its degradation by beta-TrCP.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:19217391
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:19217391
          supporting_text: Structural basis for p300 Taz2-p53 TAD1 binding and 
            modulation by phosphorylation.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:19234109
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:19234109
          supporting_text: Induction of SOX4 by DNA damage is critical for p53 
            stabilization and function.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:19255450
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:19255450
          supporting_text: Structural basis for high-affinity peptide inhibition
            of p53 interactions with MDM2 and MDMX.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:19339993
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:19339993
          supporting_text: Crosstalk between sumoylation and acetylation 
            regulates p53-dependent chromatin transcription and DNA binding.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:19345189
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:19345189
          supporting_text: A Mutant-p53/Smad complex opposes p63 to empower 
            TGFbeta-induced metastasis.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:19411066
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:19411066
          supporting_text: Regulation of XIAP translation and induction by MDM2 
            following irradiation.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:19433796
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:19433796
          supporting_text: A tumor suppressive coactivator complex of p53 
            containing ASC-2 and histone H3-lysine-4 methyltransferase MLL3 or 
            its paralogue MLL4.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:19508870
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:19508870
          supporting_text: Epub 2009 Jun 7. TOE1 interacts with p53 to modulate 
            its transactivation potential.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:19521340
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:19521340
          supporting_text: MDM4 (MDMX) localizes at the mitochondria and 
            facilitates the p53-mediated intrinsic-apoptotic pathway.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:19536131
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:19536131
          supporting_text: Differential regulation of p53 and p21 by MKRN1 E3 
            ligase controls cell cycle arrest and apoptosis.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:19556538
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:19556538
          supporting_text: Trim24 targets endogenous p53 for degradation.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:19619542
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:19619542
          supporting_text: 2009 Jul 18. Mdmx enhances p53 ubiquitination by 
            altering the substrate preference of the Mdm2 ubiquitin ligase.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:19626115
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:19626115
          supporting_text: Modulation of microRNA processing by p53.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:19651603
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:19651603
          supporting_text: Structural basis for subversion of cellular control 
            mechanisms by the adenoviral E1A oncoprotein.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:19656744
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:19656744
          supporting_text: 'Ribosomal protein S3: A multi-functional protein that
            interacts with both p53 and MDM2 through its KH domain.'
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:19680552
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:19680552
          supporting_text: CK2 is the regulator of SIRT1 substrate-binding 
            affinity, deacetylase activity and cellular response to DNA-damage.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:19684601
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:19684601
          supporting_text: Aug 16. A molecular basis for 
            phosphorylation-dependent SUMO conjugation by the E2 UBC9.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:19740107
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:19740107
          supporting_text: 2009 Sep 9. Epidermal growth factor receptor ligands 
            as new extracellular targets for the metastasis-promoting S100A4 
            protein.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:19798103
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:19798103
          supporting_text: Efficient protection and isolation of ubiquitylated 
            proteins using tandem ubiquitin-binding entities.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:19805293
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:19805293
          supporting_text: CBP and p300 are cytoplasmic E4 polyubiquitin ligases
            for p53.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:19833129
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:19833129
          supporting_text: Epub 2009 Oct 13. Polo-like kinase-1 phosphorylates 
            MDM2 at Ser260 and stimulates MDM2-mediated p53 turnover.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:19857493
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:19857493
          supporting_text: 'Epub 2009 Oct 24. Role of p53/FAK association and p53Ser46
            phosphorylation in staurosporine-mediated apoptosis: wild type versus
            mutant p53-R175H.'
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:19933256
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:19933256
          supporting_text: Nov 20. Mechanistic differences in the 
            transcriptional activation of p53 by 14-3-3 isoforms.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:20075864
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:20075864
          supporting_text: Coordinated regulation of p53 apoptotic targets BAX 
            and PUMA by SMAR1 through an identical MAR element.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:20134482
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:20134482
          supporting_text: Skp2B attenuates p53 function by inhibiting 
            prohibitin.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:20153329
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:20153329
          supporting_text: 2010 Feb 12. Reconstitution of the mitochondrial 
            Hsp70 (mortalin)-p53 interaction using purified 
            proteins--identification of additional interacting regions.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:20159018
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:20159018
          supporting_text: Epub 2010 Feb 14. Identification and characterization
            of the novel protein CCDC106 that interacts with p53 and promotes 
            its degradation.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:20167603
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:20167603
          supporting_text: 2010 Feb 18. DYRK1A and DYRK3 promote cell survival 
            through phosphorylation and activation of SIRT1.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:20173098
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:20173098
          supporting_text: RFWD3-Mdm2 ubiquitin ligase complex positively 
            regulates p53 stability in response to DNA damage.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:20206173
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:20206173
          supporting_text: '2010 Mar 3. Structure of the p53 C-terminus bound to 14-3-3:
            implications for stabilization of the p53 tetramer.'
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:20227041
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:20227041
          supporting_text: Modulation of the vitamin D3 response by 
            cancer-associated mutant p53.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:20228809
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:20228809
          supporting_text: BRD7 is a candidate tumour suppressor gene required 
            for p53 function.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:20385133
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:20385133
          supporting_text: 2010 Apr 10. p53 inhibits tumor cell invasion via the
            degradation of snail protein in hepatocellular carcinoma.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:20421506
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:20421506
          supporting_text: Mapping the physical and functional interactions 
            between the tumor suppressors p53 and BRCA2.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:20452352
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:20452352
          supporting_text: 2010 May 7. A novel hPirh2 splicing variant without 
            ubiquitin protein ligase activity interacts with p53 and is 
            down-regulated in hepatocellular carcinoma.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:20515689
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:20515689
          supporting_text: Epub 2010 May 31. MDM4 binds ligands via a mechanism 
            in which disordered regions become structured.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:20534433
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:20534433
          supporting_text: Synaptonemal complex formation and meiotic checkpoint
            signaling are linked to the lateral element protein Red1.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:20562916
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:20562916
          supporting_text: Dual-specificity phosphatase 26 is a novel p53 
            phosphatase and inhibits p53 tumor suppressor functions in human 
            neuroblastoma.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:20591429
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:20591429
          supporting_text: Epub 2010 Jun 19. S100 proteins interact with the 
            N-terminal domain of MDM2.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:20622899
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:20622899
          supporting_text: PBK/TOPK interacts with the DBD domain of tumor 
            suppressor p53 and modulates expression of transcriptional targets 
            including p21.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:20660729
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:20660729
          supporting_text: Polybromo-associated BRG1-associated factor 
            components BRD7 and BAF180 are critical regulators of p53 required 
            for induction of replicative senescence.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:20705607
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:20705607
          supporting_text: Epub 2010 Aug 12. Turning the RING domain protein 
            MdmX into an active ubiquitin-protein ligase.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:20708156
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:20708156
          supporting_text: Phosphorylation by casein kinase I promotes the 
            turnover of the Mdm2 oncoprotein via the SCF(beta-TRCP) ubiquitin 
            ligase.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:20713054
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:20713054
          supporting_text: Epub 2010 Aug 14. ARF-dependent regulation of ATM and
            p53 associated KZNF (Apak) protein activity in response to oncogenic
            stress.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:20864041
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:20864041
          supporting_text: MAGE-RING protein complexes comprise a family of E3 
            ubiquitin ligases.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:21057547
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:21057547
          supporting_text: Nov 8. AXIN is an essential co-activator for the 
            promyelocytic leukemia protein in p53 activation.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:21078964
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:21078964
          supporting_text: p53-mediated apoptosis requires inositol 
            hexakisphosphate kinase-2.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:21081126
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:21081126
          supporting_text: 2010 Nov 17. Anti-apoptotic protein TCTP controls the
            stability of the tumor suppressor p53.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:21130767
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:21130767
          supporting_text: 2010 Dec 3. SAFB1 interacts with and suppresses the 
            transcriptional activity of p53.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:21132010
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:21132010
          supporting_text: GNL3L depletion destabilizes MDM2 and induces 
            p53-dependent G2/M arrest.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:21170034
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:21170034
          supporting_text: TSPYL5 suppresses p53 levels and function by physical
            interaction with USP7.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:21170087
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:21170087
          supporting_text: Dec 20. Ribosomal protein S27-like and S27 interplay 
            with p53-MDM2 axis as a target, a substrate and a regulator.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:21245319
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:21245319
          supporting_text: Methyltransferase Set7/9 regulates p53 activity by 
            interacting with Sirtuin 1 (SIRT1).
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:21317932
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:21317932
          supporting_text: 'A new role of NUAK1: directly phosphorylating p53 and
            regulating cell proliferation.'
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:21390126
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:21390126
          supporting_text: Inactivating mutations of acetyltransferase genes in 
            B-cell lymphoma.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:21397192
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:21397192
          supporting_text: 'Interferon-inducible protein 16: insight into the interaction
            with tumor suppressor p53.'
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:21423215
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:21423215
          supporting_text: Camptothecin-induced downregulation of MLL5 
            contributes to the activation of tumor suppressor p53.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:21460856
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:21460856
          supporting_text: ATM-mediated phosphorylation activates the 
            tumor-suppressive function of B56Ξ³-PP2A.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:21471221
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:21471221
          supporting_text: 2011 Apr 6. Novel nucleolar pathway connecting 
            intracellular energy status with p53 activation.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:21513714
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:21513714
          supporting_text: Epub 2011 Apr 18. The ASPP proteins complex and 
            cooperate with p300 to modulate the transcriptional activity of p53.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:21625211
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:21625211
          supporting_text: COP9 signalosome subunit 6 stabilizes COP1, which 
            functions as an E3 ubiquitin ligase for 14-3-3Οƒ.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:21653829
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:21653829
          supporting_text: Protein interactome reveals converging molecular 
            pathways among autism disorders.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:21670263
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:21670263
          supporting_text: Structural and functional characterization of an 
            atypical activation domain in erythroid Kruppel-like factor (EKLF).
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:21726810
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:21726810
          supporting_text: Caspase-2-mediated cleavage of Mdm2 creates a 
            p53-induced positive feedback loop.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:21741598
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:21741598
          supporting_text: A Pin1/mutant p53 axis promotes aggressiveness in 
            breast cancer.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:21782458
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:21782458
          supporting_text: Jul 21. Structural basis of substrate methylation and
            inhibition of SMYD2.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:21821029
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:21821029
          supporting_text: 2011 Aug 3. Aurora-A phosphorylates hnRNPK and 
            disrupts its interaction with p53.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:21831840
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:21831840
          supporting_text: Wild-type p53 controls cell motility and invasion by 
            dual regulation of MET expression.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:21857681
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:21857681
          supporting_text: A stress response pathway regulates DNA damage 
            through Ξ²2-adrenoreceptors and Ξ²-arrestin-1.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:21892170
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:21892170
          supporting_text: Structural analysis of the interaction between Hsp90 
            and the tumor suppressor protein p53.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:21900206
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:21900206
          supporting_text: A directed protein interaction network for 
            investigating intracellular signal transduction.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:21952639
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:21952639
          supporting_text: Oct 1. NIRF constitutes a nodal point in the cell 
            cycle network and is a candidate tumor suppressor.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:21988832
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:21988832
          supporting_text: Toward an understanding of the protein interaction 
            network of the human liver.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:22056774
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:22056774
          supporting_text: Bilateral inhibition of HAUSP deubiquitinase by a 
            viral interferon regulatory factor protein.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:22085928
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:22085928
          supporting_text: Regulation of p53 stability and function by the 
            deubiquitinating enzyme USP42.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:22103682
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:22103682
          supporting_text: 2011 Dec 19. Specific domains of nucleolin interact 
            with Hdm2 and antagonize Hdm2-mediated p53 ubiquitination.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:22124327
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:22124327
          supporting_text: Positive regulation of p53 stability and activity by 
            the deubiquitinating enzyme Otubain 1.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:22265415
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:22265415
          supporting_text: Mutant p53 disrupts mammary tissue architecture via 
            the mevalonate pathway.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:22340593
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:22340593
          supporting_text: Aurora kinase-A inactivates DNA damage-induced 
            apoptosis and spindle assembly checkpoint response functions of p73.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:22451927
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:22451927
          supporting_text: Role of the translationally controlled tumor protein 
            in DNA damage sensing and repair.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:22499945
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:22499945
          supporting_text: Atg7 modulates p53 activity to regulate cell cycle 
            and survival during metabolic stress.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:22510990
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:22510990
          supporting_text: AKT-dependent phosphorylation of Niban regulates 
            nucleophosmin- and MDM2-mediated p53 stability and cell apoptosis.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:22575647
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:22575647
          supporting_text: Epub 2012 Mar 23. An autoregulatory feedback loop 
            between Mdm2 and SHP that fine tunes Mdm2 and SHP stability.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:22653443
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:22653443
          supporting_text: Mutant p53 interactome identifies nardilysin as a 
            p53R273H-specific binding partner that promotes invasion.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:22659184
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:22659184
          supporting_text: Epub 2012 May 31. Mutational analysis reveals a dual 
            role of Mdm2 acidic domain in the regulation of p53 stability.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:22723347
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:22723347
          supporting_text: Differentiated embryo-chondrocyte expressed gene 1 
            regulates p53-dependent cell survival versus cell death through 
            macrophage inhibitory cytokine-1.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:22726440
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:22726440
          supporting_text: p53 opens the mitochondrial permeability transition 
            pore to trigger necrosis.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:22810585
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:22810585
          supporting_text: Viral immune modulators perturb the human molecular 
            network by common and unique strategies.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:22810586
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:22810586
          supporting_text: Interpreting cancer genomes using systematic host 
            network perturbations by tumour virus proteins.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:22819825
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:22819825
          supporting_text: Epub 2012 Jul 20. TRIAD1 inhibits MDM2-mediated p53 
            ubiquitination and degradation.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:22975381
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:22975381
          supporting_text: 'A "twist box" code of p53 inactivation: twist box: p53
            interaction promotes p53 degradation.'
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:23010591
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:23010591
          supporting_text: 2012 Sep 23. Structural features of human histone 
            acetyltransferase p300 and its complex with p53.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:23063560
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:23063560
          supporting_text: 2012 Oct 11. HMGB1-facilitated p53 DNA binding occurs
            via HMG-Box/p53 transactivation domain interaction, regulated by the
            acidic tail.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:23092970
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:23092970
          supporting_text: SUMOylation of hnRNP-K is required for p53-mediated 
            cell-cycle arrest in response to DNA damage.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:23320542
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:23320542
          supporting_text: Evidence for self-association of the alternative 
            sigma factor Οƒ54.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:23576507
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:23576507
          supporting_text: Rotavirus-encoded nonstructural protein 1 modulates 
            cellular apoptotic machinery by targeting tumor suppressor protein 
            p53.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:23623661
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:23623661
          supporting_text: 2013 Apr 25. Restoring p53 function in human melanoma
            cells by inhibiting MDM2 and cyclin B1/CDK1-phosphorylated nuclear 
            iASPP.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:23734815
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:23734815
          supporting_text: 'Fludarabine treatment favors the retention of miR-485-3p
            by prostate cancer cells: implications for survival.'
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:23752197
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:23752197
          supporting_text: S100A4 interacts with p53 in the nucleus and promotes
            p53 degradation.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:23776060
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:23776060
          supporting_text: Identification of a second Nutlin-3 responsive 
            interaction site in the N-terminal domain of MDM2 using 
            hydrogen/deuterium exchange mass spectrometry.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:23870121
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:23870121
          supporting_text: SET1 and p300 act synergistically, through coupled 
            histone modifications, in transcriptional activation by p53.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:24207125
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:24207125
          supporting_text: 2013 Oct 24. Transient protein states in designing 
            inhibitors of the MDM2-p53 interaction.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:24219989
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:24219989
          supporting_text: Nov 12. Acetylation of p53 stimulates miRNA 
            processing and determines cell survival following genotoxic stress.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:24380853
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:24380853
          supporting_text: 2013 Dec 28. The aberrant expression and localization
            of prohibitin during apoptosis of human cholangiocarcinoma Mz-ChA-1 
            cells.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:24449765
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:24449765
          supporting_text: 'Jan 21. Activation of p53 transcriptional activity by
            SMRT: a histone deacetylase 3-independent function of a transcriptional
            corepressor.'
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:24492002
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:24492002
          supporting_text: 2014 Jan 31. VRK1 interacts with p53 forming a basal 
            complex that is activated by UV-induced DNA damage.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:24667498
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:24667498
          supporting_text: eCollection 2014 Mar.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:24722188
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:24722188
          supporting_text: Protein interaction network of alternatively spliced 
            isoforms from brain links genetic risk factors for autism.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:24814347
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:24814347
          supporting_text: May 11. The DNA-binding domain mediates both nuclear 
            and cytosolic functions of p53.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:25241761
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:25241761
          supporting_text: Oct 9. Using an in situ proximity ligation assay to 
            systematically profile endogenous protein-protein interactions in a 
            pathway network.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:25314079
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:25314079
          supporting_text: Acetylation of snail modulates the cytokinome of 
            cancer cells to enhance the recruitment of macrophages.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:25402006
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:25402006
          supporting_text: In silico prediction of physical protein interactions
            and characterization of interactome orphans.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:25502805
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:25502805
          supporting_text: eCollection 2014 Dec.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:25579814
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:25579814
          supporting_text: Jan 8. Structural plasticity of methyllysine 
            recognition by the tandem tudor domain of 53BP1.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:25609649
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:25609649
          supporting_text: Proteomic analyses reveal distinct 
            chromatin-associated and soluble transcription factor complexes.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:25651062
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:25651062
          supporting_text: An acetyl-methyl switch drives a conformational 
            change in p53.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:25670079
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:25670079
          supporting_text: 14-3-3ΞΆ turns TGF-Ξ²'s function from tumor suppressor 
            to metastasis promoter in breast cancer by contextual changes of 
            Smad partners from p53 to Gli2.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:25837623
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:25837623
          supporting_text: eCollection 2015 Apr.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:25857266
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:25857266
          supporting_text: 2015 Apr 10. Pontin, a new mutant p53-binding 
            protein, promotes gain-of-function of mutant p53.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:26302407
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:26302407
          supporting_text: Combined CSL and p53 downregulation promotes 
            cancer-associated fibroblast activation.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:26331536
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:26331536
          supporting_text: Gain-of-function p53 mutants co-opt chromatin 
            pathways to drive cancer growth.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:26789255
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:26789255
          supporting_text: Structure of the E6/E6AP/p53 complex required for 
            HPV-mediated degradation of p53.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:27107012
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:27107012
          supporting_text: Pooled-matrix protein interaction screens using 
            Barcode Fusion Genetics.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:27519799
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:27519799
          supporting_text: p53 down-regulates SARS coronavirus replication and 
            is targeted by the SARS-unique domain and PLpro via E3 ubiquitin 
            ligase RCHY1.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:27605672
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:27605672
          supporting_text: Nov 15. Mitogen-Activated Protein Kinase Kinase 2, a 
            Novel E2-Interacting Protein, Promotes the Growth of Classical Swine
            Fever Virus via Attenuation of the JAK-STAT Signaling Pathway.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:29187402
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:29187402
          supporting_text: Epub 2017 Nov 29. The E3 Ligase RING1 Targets p53 for
            Degradation and Promotes Cancer Cell Proliferation and Survival.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:29340707
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:29340707
          supporting_text: Epub 2018 Jan 16. VRK1 and AURKB form a complex that 
            cross inhibit their kinase activity and the phosphorylation of 
            histone H3 in the progression of mitosis.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:29997244
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:29997244
          supporting_text: 'LuTHy: a double-readout bioluminescence-based two-hybrid
            technology for quantitative mapping of protein-protein interactions in
            mammalian cells.'
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:31467278
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:31467278
          supporting_text: Maximizing binary interactome mapping with a minimal 
            number of assays.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:31511497
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:31511497
          supporting_text: miR-146a attenuates apoptosis and modulates autophagy
            by targeting TAF9b/P53 pathway in doxorubicin-induced 
            cardiotoxicity.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:31515488
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:31515488
          supporting_text: Extensive disruption of protein interactions by 
            genetic variants across the allele frequency spectrum in human 
            populations.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:31837246
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:31837246
          supporting_text: High-throughput competitive fluorescence polarization
            assay reveals functional redundancy in the S100 protein family.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:32606738
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:32606738
          supporting_text: eCollection 2020. P55PIK Regulates P53-Dependent 
            Apoptosis in Cancer Cells by Interacting with P53 DNA-Specific 
            Domain.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:32814053
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:32814053
          supporting_text: Interactome Mapping Provides a Network of 
            Neurodegenerative Disease Proteins and Uncovers Widespread Protein 
            Aggregation in Affected Brains.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:33961781
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:33961781
          supporting_text: 2021 May 6. Dual proteome-scale networks reveal 
            cell-specific remodeling of the human interactome.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:34316702
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:34316702
          supporting_text: 2021 Mar. Elucidation of the BMI1 interactome 
            identifies novel regulatory roles in glioblastoma.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:34591612
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:34591612
          supporting_text: Oct 1. A protein interaction landscape of breast 
            cancer.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:34591642
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:34591642
          supporting_text: Oct 1. A protein network map of head and neck cancer 
            reveals PIK3CA mutant drug sensitivity.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:35044719
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:35044719
          supporting_text: Proteome-scale mapping of binding sites in the 
            unstructured regions of the human proteome.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:35140242
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:35140242
          supporting_text: Human transcription factor protein interaction 
            networks.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:35271311
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:35271311
          supporting_text: '2022 Mar 11. OpenCell: Endogenous tagging for the cartography
            of human cellular organization.'
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:35512704
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:35512704
          supporting_text: 2022 May 4. Systematic discovery of mutation-directed
            neo-protein-protein interactions in cancer.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:36931259
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:36931259
          supporting_text: A central chaperone-like role for 14-3-3 proteins in 
            human cells.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:39009827
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:39009827
          supporting_text: 2024 Jul 15. Proteome-scale characterisation of 
            motif-based interactome rewiring by disease mutations.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:7799929
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:7799929
          supporting_text: Two domains of p53 interact with the TATA-binding 
            protein, and the adenovirus 13S E1A protein disrupts the 
            association, relieving p53-mediated transcriptional repression.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:8207801
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:8207801
          supporting_text: Inhibition of p53 DNA binding by human papillomavirus
            E6 proteins.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:8344494
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:8344494
          supporting_text: Identification of mutations in p53 that affect its 
            binding to SV40 large T antigen by using the yeast two-hybrid 
            system.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:8875926
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:8875926
          supporting_text: Structure of the p53 tumor suppressor bound to the 
            ankyrin and SH3 domains of 53BP2.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:8875929
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:8875929
          supporting_text: Structure of the MDM2 oncoprotein bound to the p53 
            tumor suppressor transactivation domain.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:9050995
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:9050995
          supporting_text: Identification of p53 unbound to T-antigen in human 
            cells transformed by simian virus 40 T-antigen.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:9188558
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:9188558
          supporting_text: Nuclear localization of the NS3 protein of hepatitis 
            C virus and factors affecting the localization.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:9194564
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:9194564
          supporting_text: Synergistic activation of transcription by CBP and 
            p53.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:9380510
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:9380510
          supporting_text: Interaction of p53 with the human Rad51 protein.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:9472015
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:9472015
          supporting_text: High mobility group protein-1 (HMG-1) is a unique 
            activator of p53.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:9807817
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:9807817
          supporting_text: An Arabidopsis immunophilin, AtFKBP12, binds to 
            AtFIP37 (FKBP interacting protein) in an interaction that is 
            disrupted by FK506.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:9827557
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:9827557
          supporting_text: Complex formation of the nonstructural protein 3 of 
            hepatitis C virus with the p53 tumor suppressor.
  - term:
      id: GO:0042802
      label: identical protein binding
    evidence_type: IPI
    original_reference_id: PMID:10876243
    review:
      summary: p53 binds to itself to form tetramers through its oligomerization
        domain, essential for DNA binding.
      action: ACCEPT
      reason: Core structural feature - p53 tetramerization through identical 
        protein binding is essential for function.
      supported_by: &id007
        - reference_id: PDB:1C26
          supporting_text: Crystal structure of p53 tetramerization domain
        - reference_id: PMID:10876243
          supporting_text: Structure of the negative regulatory domain of p53 
            bound to S100B(betabeta).
        - reference_id: PMID:14759370
          supporting_text: Structural mechanism of the bromodomain of the 
            coactivator CBP in p53 transcriptional activation.
        - reference_id: PMID:14985081
          supporting_text: Hepatitis C virus core protein interacts with 
            p53-binding protein, 53BP2/Bbp/ASPP2, and inhibits p53-mediated 
            apoptosis.
        - reference_id: PMID:16291740
          supporting_text: 2005 Nov 15. Charge modification at multiple 
            C-terminal lysine residues regulates p53 oligomerization and its 
            nucleus-cytoplasm trafficking.
        - reference_id: PMID:16461914
          supporting_text: Core domain interactions in full-length p53 in 
            solution.
        - reference_id: PMID:17612295
          supporting_text: Yeast split-ubiquitin-based cytosolic screening 
            system to detect interactions between transcriptionally active 
            proteins.
        - reference_id: PMID:17620598
          supporting_text: Quaternary structures of tumor suppressor p53 and a 
            specific p53 DNA complex.
        - reference_id: PMID:18087040
          supporting_text: Regulation of p53 tetramerization and nuclear export 
            by ARC.
        - reference_id: PMID:19339993
          supporting_text: Crosstalk between sumoylation and acetylation 
            regulates p53-dependent chromatin transcription and DNA binding.
        - reference_id: PMID:19667193
          supporting_text: Ultraslow oligomerization equilibria of p53 and its 
            implications.
        - reference_id: PMID:20004160
          supporting_text: Averaging of electron subtomograms and random conical
            tilt reconstructions through likelihood optimization.
        - reference_id: PMID:20159469
          supporting_text: Crystal structure of the p53 core domain bound to a 
            full consensus site as a self-assembled tetramer.
        - reference_id: PMID:20364130
          supporting_text: Apr 4. Diversity in DNA recognition by p53 revealed 
            by crystal structures with Hoogsteen base pairs.
        - reference_id: PMID:21178074
          supporting_text: Electron microscopy studies on the quaternary 
            structure of p53 reveal different binding modes for p53 tetramers in
            complex with DNA.
        - reference_id: PMID:21522129
          supporting_text: An induced fit mechanism regulates p53 DNA binding 
            kinetics to confer sequence specificity.
        - reference_id: PMID:21988832
          supporting_text: Toward an understanding of the protein interaction 
            network of the human liver.
        - reference_id: PMID:22653443
          supporting_text: Mutant p53 interactome identifies nardilysin as a 
            p53R273H-specific binding partner that promotes invasion.
        - reference_id: PMID:22972749
          supporting_text: Domain-domain interactions in full-length p53 and a 
            specific DNA complex probed by methyl NMR spectroscopy.
        - reference_id: PMID:25402006
          supporting_text: In silico prediction of physical protein interactions
            and characterization of interactome orphans.
        - reference_id: PMID:25609649
          supporting_text: Proteomic analyses reveal distinct 
            chromatin-associated and soluble transcription factor complexes.
        - reference_id: PMID:31837246
          supporting_text: High-throughput competitive fluorescence polarization
            assay reveals functional redundancy in the S100 protein family.
        - reference_id: PMID:35512704
          supporting_text: 2022 May 4. Systematic discovery of mutation-directed
            neo-protein-protein interactions in cancer.
        - reference_id: PMID:15629713
          supporting_text: Binding of natively unfolded HIF-1alpha ODD domain to
            p53.
        - reference_id: PMID:19011621
          supporting_text: Arginine methylation regulates the p53 response.
  - term:
      id: GO:0042802
      label: identical protein binding
    evidence_type: IPI
    original_reference_id: PMID:14759370
    review:
      summary: p53 binds to itself to form tetramers through its oligomerization
        domain, essential for DNA binding.
      action: ACCEPT
      reason: Core structural feature - p53 tetramerization through identical 
        protein binding is essential for function.
      supported_by: *id007
  - term:
      id: GO:0042802
      label: identical protein binding
    evidence_type: IPI
    original_reference_id: PMID:14985081
    review:
      summary: p53 binds to itself to form tetramers through its oligomerization
        domain, essential for DNA binding.
      action: ACCEPT
      reason: Core structural feature - p53 tetramerization through identical 
        protein binding is essential for function.
      supported_by: *id007
  - term:
      id: GO:0042802
      label: identical protein binding
    evidence_type: IPI
    original_reference_id: PMID:16291740
    review:
      summary: p53 binds to itself to form tetramers through its oligomerization
        domain, essential for DNA binding.
      action: ACCEPT
      reason: Core structural feature - p53 tetramerization through identical 
        protein binding is essential for function.
      supported_by: *id007
  - term:
      id: GO:0042802
      label: identical protein binding
    evidence_type: IPI
    original_reference_id: PMID:16461914
    review:
      summary: p53 binds to itself to form tetramers through its oligomerization
        domain, essential for DNA binding.
      action: ACCEPT
      reason: Core structural feature - p53 tetramerization through identical 
        protein binding is essential for function.
      supported_by: *id007
  - term:
      id: GO:0042802
      label: identical protein binding
    evidence_type: IPI
    original_reference_id: PMID:17612295
    review:
      summary: p53 binds to itself to form tetramers through its oligomerization
        domain, essential for DNA binding.
      action: ACCEPT
      reason: Core structural feature - p53 tetramerization through identical 
        protein binding is essential for function.
      supported_by: *id007
  - term:
      id: GO:0042802
      label: identical protein binding
    evidence_type: IPI
    original_reference_id: PMID:17620598
    review:
      summary: p53 binds to itself to form tetramers through its oligomerization
        domain, essential for DNA binding.
      action: ACCEPT
      reason: Core structural feature - p53 tetramerization through identical 
        protein binding is essential for function.
      supported_by: *id007
  - term:
      id: GO:0042802
      label: identical protein binding
    evidence_type: IPI
    original_reference_id: PMID:18087040
    review:
      summary: p53 binds to itself to form tetramers through its oligomerization
        domain, essential for DNA binding.
      action: ACCEPT
      reason: Core structural feature - p53 tetramerization through identical 
        protein binding is essential for function.
      supported_by: *id007
  - term:
      id: GO:0042802
      label: identical protein binding
    evidence_type: IPI
    original_reference_id: PMID:19339993
    review:
      summary: p53 binds to itself to form tetramers through its oligomerization
        domain, essential for DNA binding.
      action: ACCEPT
      reason: Core structural feature - p53 tetramerization through identical 
        protein binding is essential for function.
      supported_by: *id007
  - term:
      id: GO:0042802
      label: identical protein binding
    evidence_type: IPI
    original_reference_id: PMID:19667193
    review:
      summary: p53 binds to itself to form tetramers through its oligomerization
        domain, essential for DNA binding.
      action: ACCEPT
      reason: Core structural feature - p53 tetramerization through identical 
        protein binding is essential for function.
      supported_by: *id007
  - term:
      id: GO:0042802
      label: identical protein binding
    evidence_type: IPI
    original_reference_id: PMID:20004160
    review:
      summary: p53 binds to itself to form tetramers through its oligomerization
        domain, essential for DNA binding.
      action: ACCEPT
      reason: Core structural feature - p53 tetramerization through identical 
        protein binding is essential for function.
      supported_by: *id007
  - term:
      id: GO:0042802
      label: identical protein binding
    evidence_type: IPI
    original_reference_id: PMID:20159469
    review:
      summary: p53 binds to itself to form tetramers through its oligomerization
        domain, essential for DNA binding.
      action: ACCEPT
      reason: Core structural feature - p53 tetramerization through identical 
        protein binding is essential for function.
      supported_by: *id007
  - term:
      id: GO:0042802
      label: identical protein binding
    evidence_type: IPI
    original_reference_id: PMID:20364130
    review:
      summary: p53 binds to itself to form tetramers through its oligomerization
        domain, essential for DNA binding.
      action: ACCEPT
      reason: Core structural feature - p53 tetramerization through identical 
        protein binding is essential for function.
      supported_by: *id007
  - term:
      id: GO:0042802
      label: identical protein binding
    evidence_type: IPI
    original_reference_id: PMID:21178074
    review:
      summary: p53 binds to itself to form tetramers through its oligomerization
        domain, essential for DNA binding.
      action: ACCEPT
      reason: Core structural feature - p53 tetramerization through identical 
        protein binding is essential for function.
      supported_by: *id007
  - term:
      id: GO:0042802
      label: identical protein binding
    evidence_type: IPI
    original_reference_id: PMID:21522129
    review:
      summary: p53 binds to itself to form tetramers through its oligomerization
        domain, essential for DNA binding.
      action: ACCEPT
      reason: Core structural feature - p53 tetramerization through identical 
        protein binding is essential for function.
      supported_by: *id007
  - term:
      id: GO:0042802
      label: identical protein binding
    evidence_type: IPI
    original_reference_id: PMID:21988832
    review:
      summary: p53 binds to itself to form tetramers through its oligomerization
        domain, essential for DNA binding.
      action: ACCEPT
      reason: Core structural feature - p53 tetramerization through identical 
        protein binding is essential for function.
      supported_by: *id007
  - term:
      id: GO:0042802
      label: identical protein binding
    evidence_type: IPI
    original_reference_id: PMID:22653443
    review:
      summary: p53 binds to itself to form tetramers through its oligomerization
        domain, essential for DNA binding.
      action: ACCEPT
      reason: Core structural feature - p53 tetramerization through identical 
        protein binding is essential for function.
      supported_by: *id007
  - term:
      id: GO:0042802
      label: identical protein binding
    evidence_type: IPI
    original_reference_id: PMID:22972749
    review:
      summary: p53 binds to itself to form tetramers through its oligomerization
        domain, essential for DNA binding.
      action: ACCEPT
      reason: Core structural feature - p53 tetramerization through identical 
        protein binding is essential for function.
      supported_by: *id007
  - term:
      id: GO:0042802
      label: identical protein binding
    evidence_type: IPI
    original_reference_id: PMID:25402006
    review:
      summary: p53 binds to itself to form tetramers through its oligomerization
        domain, essential for DNA binding.
      action: ACCEPT
      reason: Core structural feature - p53 tetramerization through identical 
        protein binding is essential for function.
      supported_by: *id007
  - term:
      id: GO:0042802
      label: identical protein binding
    evidence_type: IPI
    original_reference_id: PMID:25609649
    review:
      summary: p53 binds to itself to form tetramers through its oligomerization
        domain, essential for DNA binding.
      action: ACCEPT
      reason: Core structural feature - p53 tetramerization through identical 
        protein binding is essential for function.
      supported_by: *id007
  - term:
      id: GO:0042802
      label: identical protein binding
    evidence_type: IPI
    original_reference_id: PMID:31837246
    review:
      summary: p53 binds to itself to form tetramers through its oligomerization
        domain, essential for DNA binding.
      action: ACCEPT
      reason: Core structural feature - p53 tetramerization through identical 
        protein binding is essential for function.
      supported_by: *id007
  - term:
      id: GO:0042802
      label: identical protein binding
    evidence_type: IPI
    original_reference_id: PMID:35512704
    review:
      summary: p53 binds to itself to form tetramers through its oligomerization
        domain, essential for DNA binding.
      action: ACCEPT
      reason: Core structural feature - p53 tetramerization through identical 
        protein binding is essential for function.
      supported_by: *id007
  - term:
      id: GO:0017053
      label: transcription repressor complex
    evidence_type: IPI
    original_reference_id: PMID:18677113
    review:
      summary: p53 functions within transcriptional regulator complexes to 
        control gene expression.
      action: ACCEPT
      reason: Core function - p53 forms complexes with transcriptional machinery
        and co-factors.
      supported_by: &id008
        - reference_id: TP53-deep-research
          supporting_text: Complex formation with transcriptional co-activators 
            (CBP/p300, TFIID)
        - reference_id: PMID:18677113
          supporting_text: Structure of the human Mdmx protein bound to the p53 
            tumor suppressor transactivation domain.
        - reference_id: PMID:8875929
          supporting_text: Structure of the MDM2 oncoprotein bound to the p53 
            tumor suppressor transactivation domain.
  - term:
      id: GO:0017053
      label: transcription repressor complex
    evidence_type: IPI
    original_reference_id: PMID:8875929
    review:
      summary: p53 functions within transcriptional regulator complexes to 
        control gene expression.
      action: ACCEPT
      reason: Core function - p53 forms complexes with transcriptional machinery
        and co-factors.
      supported_by: *id008
  - term:
      id: GO:0045892
      label: negative regulation of DNA-templated transcription
    evidence_type: NAS
    original_reference_id: PMID:16492744
    review:
      summary: p53 negatively regulates transcription of various target genes 
        including anti-apoptotic and cell cycle genes.
      action: ACCEPT
      reason: Core function - p53 acts as both a transcriptional activator and 
        repressor.
      supported_by: &id009
        - reference_id: TP53-deep-research
          supporting_text: 'Repression: Downregulates anti-apoptotic genes (BCL2),
            cell cycle progression genes'
        - reference_id: PMID:16492744
          supporting_text: Mdm4 and Mdm2 cooperate to inhibit p53 activity in 
            proliferating and quiescent cells in vivo.
        - reference_id: PMID:9271120
          supporting_text: 'Repression of p53-mediated transcription by MDM2: a dual
            mechanism.'
        - reference_id: PMID:23629966
          supporting_text: Deacetylation of p53 induces autophagy by suppressing
            Bmf expression.
        - reference_id: PMID:24051492
          supporting_text: p53 regulates Period2 expression and the circadian 
            clock.
  - term:
      id: GO:0045892
      label: negative regulation of DNA-templated transcription
    evidence_type: IDA
    original_reference_id: PMID:9271120
    review:
      summary: p53 negatively regulates transcription of various target genes 
        including anti-apoptotic and cell cycle genes.
      action: ACCEPT
      reason: Core function - p53 acts as both a transcriptional activator and 
        repressor.
      supported_by: *id009
  - term:
      id: GO:0000977
      label: RNA polymerase II transcription regulatory region sequence-specific
        DNA binding
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 binds to RNA polymerase II cis-regulatory regions in target 
        gene promoters.
      action: ACCEPT
      reason: Core molecular function - sequence-specific DNA binding is 
        essential for p53 transcription factor activity.
      supported_by:
        - reference_id: TP53-deep-research
          supporting_text: TP53 is fundamentally a transcription factor that 
            binds sequence-specifically to p53 response elements
  - term:
      id: GO:0000978
      label: RNA polymerase II cis-regulatory region sequence-specific DNA 
        binding
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 binds sequence-specifically to p53 response elements in 
        cis-regulatory regions of target genes.
      action: ACCEPT
      reason: Core molecular function - p53's DNA-binding domain binds to 
        consensus p53 response elements (p53REs) with high specificity.
      supported_by: *id010
  - term:
      id: GO:0001228
      label: DNA-binding transcription activator activity, RNA polymerase 
        II-specific
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 activates transcription through binding to promoter 
        sequences.
      action: ACCEPT
      reason: Core molecular function - p53 is a transcriptional activator that 
        binds to p53 response elements.
      supported_by: &id026
        - reference_id: TP53-deep-research
          supporting_text: TP53 is fundamentally a transcription factor that 
            binds sequence-specifically to p53 response elements
        - reference_id: PMID:22578566
          supporting_text: Apr 19. Oncosuppressive role of p53-induced miR-205 
            in triple negative breast cancer.
        - reference_id: PMID:17145718
          supporting_text: Brn-3b enhances the pro-apoptotic effects of p53 but 
            not its induction of cell cycle arrest by cooperating in 
            trans-activation of bax expression.
        - reference_id: PMID:12609999
          supporting_text: 2003 Feb 27. The activation domains, the proline-rich
            domain, and the C-terminal basic domain in p53 are necessary for 
            acetylation of histones on the proximal p21 promoter and interaction
            with p300/CREB-binding protein.
        - reference_id: PMID:17146433
          supporting_text: Dec 4. RGC32, a novel p53-inducible gene, is located 
            on centrosomes during mitosis and results in G2/M arrest.
  - term:
      id: GO:0003682
      label: chromatin binding
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 binds chromatin at p53 response elements in target gene 
        promoters.
      action: ACCEPT
      reason: Core molecular function - chromatin binding is essential for p53's
        transcriptional regulation.
      supported_by: &id021
        - reference_id: PDB:3TS8
          supporting_text: Crystal structure of p53 tetramer bound to DNA
        - reference_id: PMID:26334721
          supporting_text: 2015 Sep 3. Jmjd5 functions as a regulator of p53 
            signaling during mouse embryogenesis.
        - reference_id: PMID:16322561
          supporting_text: NIR is a novel INHAT repressor that modulates the 
            transcriptional activity of p53.
        - reference_id: PMID:17599062
          supporting_text: Jun 28. CDIP, a novel pro-apoptotic gene, regulates 
            TNFalpha-mediated apoptosis in a p53-dependent manner.
  - term:
      id: GO:0005657
      label: replication fork
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 is a component of replication fork DNA repair machinery.
      action: ACCEPT
      reason: p53 coordinates DNA repair at replication forks during DNA damage 
        response.
  - term:
      id: GO:0005739
      label: mitochondrion
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 translocates to mitochondria to directly promote apoptosis 
        through BAX activation.
      action: ACCEPT
      reason: Important localization for non-transcriptional apoptosis - p53 can
        directly induce MOMP at mitochondria.
      supported_by: &id029
        - reference_id: TP53-deep-research
          supporting_text: 'Non-transcriptional: Direct mitochondrial translocation
            and interaction with BCL-2 family proteins'
        - reference_id: PMID:25168243
          supporting_text: Aug 29. Cofactor Strap regulates oxidative 
            phosphorylation and mitochondrial p53 activity through ATP synthase.
        - reference_id: PMID:24101517
          supporting_text: Mitochondrial disulfide relay mediates translocation 
            of p53 and partitions its subcellular activity.
        - reference_id: PMID:12667443
          supporting_text: p53 has a direct apoptogenic role at the 
            mitochondria.
  - term:
      id: GO:0005829
      label: cytosol
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 localizes to cytosol as part of its nucleo-cytoplasmic 
        shuttling.
      action: ACCEPT
      reason: p53 shuttles between nucleus and cytoplasm, with cytosolic 
        localization important for regulation.
      supported_by: &id018
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: 'SUBCELLULAR LOCATION: Nucleus. Cytoplasm'
        - reference_id: PMID:24101517
          supporting_text: Mitochondrial disulfide relay mediates translocation 
            of p53 and partitions its subcellular activity.
        - reference_id: PMID:14963330
          supporting_text: Direct activation of Bax by p53 mediates 
            mitochondrial membrane permeabilization and apoptosis.
  - term:
      id: GO:0007623
      label: circadian rhythm
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 may influence circadian rhythms but this is not a primary 
        function.
      action: KEEP_AS_NON_CORE
      reason: Non-core function - p53 has connections to circadian biology.
  - term:
      id: GO:0008285
      label: negative regulation of cell population proliferation
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 negatively regulates cell proliferation as a fundamental 
        tumor suppressor mechanism.
      action: ACCEPT
      reason: Core function - p53 inhibits proliferation through cell cycle 
        arrest.
      supported_by: *id011
  - term:
      id: GO:0008340
      label: determination of adult lifespan
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 may influence lifespan through its regulation of senescence 
        and apoptosis.
      action: KEEP_AS_NON_CORE
      reason: Non-core function - while p53 influences aging, this is secondary 
        to its tumor suppressor function.
  - term:
      id: GO:0031625
      label: ubiquitin protein ligase binding
    evidence_type: IEA
    original_reference_id: GO_REF:0000120
    review:
      summary: Generic 'ubiquitin protein ligase binding' is less informative 
        than specific MDM2/MDM4 interactions.
      action: REMOVE
      reason: While p53 binds MDM2 (E3 ligase), this generic term is 
        uninformative. The specific MDM2 interaction is well-documented 
        elsewhere.
  - term:
      id: GO:0034644
      label: cellular response to UV
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 responds to UV-induced DNA damage as part of its DNA damage 
        response.
      action: ACCEPT
      reason: Core function - UV radiation is a classic p53-activating stress.
      supported_by: &id033
        - reference_id: GO:0009411
          supporting_text: response to UV
        - reference_id: PMID:16213212
          supporting_text: Regulation of p53 translation and induction after DNA
            damage by ribosomal protein L26 and nucleolin.
  - term:
      id: GO:0035033
      label: histone deacetylase regulator activity
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 undergoes deacetylation by histone deacetylases including 
        HDAC1.
      action: ACCEPT
      reason: Core regulatory mechanism - p53 deacetylation by HDACs regulates 
        its activity.
      supported_by:
        - reference_id: TP53-deep-research
          supporting_text: Acetylation/deacetylation regulates p53 function
  - term:
      id: GO:0035861
      label: site of double-strand break
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 is involved in ventral column development in spinal cord.
      action: KEEP_AS_NON_CORE
      reason: Non-core tissue-specific developmental function.
  - term:
      id: GO:0042771
      label: intrinsic apoptotic signaling pathway in response to DNA damage by 
        p53 class mediator
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 is THE p53 class mediator that induces intrinsic apoptosis in
        response to DNA damage through BAX, PUMA, NOXA activation.
      action: ACCEPT
      reason: Absolutely core function - this term specifically describes p53's 
        role as the central mediator of DNA damage-induced apoptosis.
      supported_by: *id004
  - term:
      id: GO:0043073
      label: germ cell nucleus
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 is involved in germ cell development but is not essential.
      action: KEEP_AS_NON_CORE
      reason: Non-core developmental function.
  - term:
      id: GO:0043153
      label: entrainment of circadian clock by photoperiod
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 enters the nucleus to function as a transcription factor.
      action: ACCEPT
      reason: Core process - nuclear entry is essential for p53's transcription 
        factor activity.
      supported_by: &id038
        - reference_id: TP53-deep-research
          supporting_text: 'Nuclear Localization Signals (NLS): Function: Nuclear
            import and localization'
  - term:
      id: GO:0045892
      label: negative regulation of DNA-templated transcription
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 negatively regulates transcription of various target genes 
        including anti-apoptotic and cell cycle genes.
      action: ACCEPT
      reason: Core function - p53 acts as both a transcriptional activator and 
        repressor.
      supported_by: *id009
  - term:
      id: GO:0048512
      label: circadian behavior
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 is involved in organ development but is not essential for 
        development.
      action: KEEP_AS_NON_CORE
      reason: Non-core developmental function - p53 knockout mice develop 
        normally.
  - term:
      id: GO:0060333
      label: type II interferon-mediated signaling pathway
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 may influence interferon-gamma signaling but this is not a 
        primary function.
      action: KEEP_AS_NON_CORE
      reason: Non-core immunomodulatory function.
  - term:
      id: GO:0062100
      label: positive regulation of programmed necrotic cell death
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 regulates intrinsic apoptotic signaling in response to 
        various DNA damage types.
      action: ACCEPT
      reason: Core function - p53 responds to diverse DNA damage to trigger 
        apoptosis.
  - term:
      id: GO:0070059
      label: intrinsic apoptotic signaling pathway in response to endoplasmic 
        reticulum stress
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 is phosphorylated on serine residues including Ser15, Ser20, 
        Ser46, Ser392.
      action: ACCEPT
      reason: Core regulatory mechanism - serine phosphorylation is essential 
        for p53 activation.
      supported_by:
        - reference_id: TP53-deep-research
          supporting_text: 'Ser15, Thr18, Ser20: N-terminal phosphorylation by ATM/ATR,
            disrupts MDM2 binding'
  - term:
      id: GO:0070245
      label: positive regulation of thymocyte apoptotic process
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 responds to positive regulation by stress-induced 
        post-translational modifications.
      action: ACCEPT
      reason: p53 is regulated by extensive post-translational modifications 
        that enhance its activity.
      supported_by: &id042
        - reference_id: TP53-deep-research
          supporting_text: p53 can be modified at over 60 of its 393 residues
  - term:
      id: GO:0071479
      label: cellular response to ionizing radiation
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 responds to UV-A radiation-induced DNA damage.
      action: ACCEPT
      reason: Core DNA damage response function - UV radiation is a classic p53 
        activator.
      supported_by: &id043
        - reference_id: GO:0009411
          supporting_text: response to UV
        - reference_id: PMID:20160708
          supporting_text: Feedback between p21 and reactive oxygen production 
            is necessary for cell senescence.
  - term:
      id: GO:0097371
      label: MDM2/MDM4 family protein binding
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 undergoes mitochondrial translocation to directly promote 
        apoptosis.
      action: ACCEPT
      reason: Important function - p53 can induce apoptosis through 
        non-transcriptional mitochondrial mechanisms.
      supported_by:
        - reference_id: TP53-deep-research
          supporting_text: Direct mitochondrial translocation and interaction 
            with BCL-2 family proteins
  - term:
      id: GO:2000774
      label: positive regulation of cellular senescence
    evidence_type: IEA
    original_reference_id: GO_REF:0000107
    review:
      summary: p53 positively regulates cellular senescence as an alternative to
        apoptosis.
      action: ACCEPT
      reason: Core function - senescence induction is a major tumor suppressor 
        mechanism of p53.
      supported_by: &id024
        - reference_id: TP53-deep-research
          supporting_text: p53 induces senescence through p21 and other targets 
            as an irreversible cell cycle arrest mechanism
        - reference_id: PMID:29474172
          supporting_text: Haploinsufficiency of Trp53 dramatically extends the 
            lifespan of Sirt6-deficient mice.
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: IDA
    original_reference_id: PMID:12915590
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: &id017
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: 'SUBCELLULAR LOCATION: Nucleus'
        - reference_id: PMID:12915590
          supporting_text: Aug 12. Cellular stress and DNA damage invoke 
            temporally distinct Mdm2, p53 and PML complexes and damage-specific 
            nuclear relocalization.
        - reference_id: PMID:11080164
          supporting_text: Regulation of p53 activity in nuclear bodies by a 
            specific PML isoform.
  - term:
      id: GO:0072331
      label: signal transduction by p53 class mediator
    evidence_type: IDA
    original_reference_id: PMID:25384516
    review:
      summary: p53 IS the p53 class mediator that transduces signals in response
        to stress.
      action: ACCEPT
      reason: Absolutely core function - this term specifically describes p53's 
        central role in stress signaling.
      supported_by: *id012
  - term:
      id: GO:1990841
      label: promoter-specific chromatin binding
    evidence_type: IDA
    original_reference_id: PMID:24652652
    review:
      summary: p53 binds to promoter-specific chromatin regions containing p53 
        response elements.
      action: ACCEPT
      reason: Core molecular function - p53 specifically binds to promoter 
        regions of target genes through sequence-specific DNA binding.
      supported_by: *id013
  - term:
      id: GO:0072331
      label: signal transduction by p53 class mediator
    evidence_type: IDA
    original_reference_id: PMID:18549481
    review:
      summary: p53 IS the p53 class mediator that transduces signals in response
        to stress.
      action: ACCEPT
      reason: Absolutely core function - this term specifically describes p53's 
        central role in stress signaling.
      supported_by: *id012
  - term:
      id: GO:0000785
      label: chromatin
    evidence_type: IDA
    original_reference_id: PMID:24289924
    review:
      summary: p53 associates with chromatin at target gene promoters to 
        regulate transcription.
      action: ACCEPT
      reason: Core molecular function - p53 binds to chromatin at p53 response 
        elements to regulate target gene expression.
      supported_by: *id014
  - term:
      id: GO:0003700
      label: DNA-binding transcription factor activity
    evidence_type: IMP
    original_reference_id: PMID:16479015
    review:
      summary: p53 is a well-characterized DNA-binding transcription factor that
        activates and represses target genes.
      action: ACCEPT
      reason: Core molecular function - p53 is one of the best-characterized 
        transcription factors, regulating >500 target genes.
      supported_by: *id015
  - term:
      id: GO:0030330
      label: DNA damage response, signal transduction by p53 class mediator
    evidence_type: IDA
    original_reference_id: PMID:29681526
    review:
      summary: p53 mediates DNA damage response signal transduction as THE p53 
        class mediator.
      action: ACCEPT
      reason: Absolutely core function - p53 is the defining member of the p53 
        class mediator family.
      supported_by: *id016
  - term:
      id: GO:0010628
      label: positive regulation of gene expression
    evidence_type: ISS
    original_reference_id: GO_REF:0000024
    review:
      summary: p53 positively regulates gene expression of numerous target 
        genes.
      action: ACCEPT
      reason: Core transcriptional function - p53 is a transcriptional 
        activator.
      supported_by: &id023
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: Multifunctional transcription factor
        - reference_id: PMID:26100857
          supporting_text: A metabolic stress-inducible miR-34a-HNF4Ξ± pathway 
            regulates lipid and lipoprotein metabolism.
        - reference_id: PMID:20332243
          supporting_text: 2010 Mar 23. MicroRNA145 targets BNIP3 and suppresses
            prostate cancer progression.
        - reference_id: PMID:15314173
          supporting_text: Ribosomal protein L23 activates p53 by inhibiting 
            MDM2 function in response to ribosomal perturbation but not to 
            translation inhibition.
        - reference_id: PMID:19160485
          supporting_text: A ribosomal protein L23-nucleophosmin circuit 
            coordinates Mizl function with cell growth.
  - term:
      id: GO:0001223
      label: transcription coactivator binding
    evidence_type: IPI
    original_reference_id: PMID:11950834
    review:
      summary: p53 functions as a transcription activator at p53 response 
        elements.
      action: ACCEPT
      reason: Core molecular function - p53 activates transcription at 
        cis-regulatory elements.
      supported_by:
        - reference_id: TP53-deep-research
          supporting_text: p53 binds sequence-specifically to p53 response 
            elements
        - reference_id: PMID:11950834
          supporting_text: 2002 Apr 11. SWI/SNF complex interacts with tumor 
            suppressor p53 and is necessary for the activation of p53-mediated 
            transcription.
  - term:
      id: GO:0030330
      label: DNA damage response, signal transduction by p53 class mediator
    evidence_type: IDA
    original_reference_id: PMID:15149599
    review:
      summary: p53 mediates DNA damage response signal transduction as THE p53 
        class mediator.
      action: ACCEPT
      reason: Absolutely core function - p53 is the defining member of the p53 
        class mediator family.
      supported_by: *id016
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: IDA
    original_reference_id: GO_REF:0000052
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005829
      label: cytosol
    evidence_type: IDA
    original_reference_id: GO_REF:0000052
    review:
      summary: p53 localizes to cytosol as part of its nucleo-cytoplasmic 
        shuttling.
      action: ACCEPT
      reason: p53 shuttles between nucleus and cytoplasm, with cytosolic 
        localization important for regulation.
      supported_by: *id018
  - term:
      id: GO:0000981
      label: DNA-binding transcription factor activity, RNA polymerase 
        II-specific
    evidence_type: IDA
    original_reference_id: PMID:34381247
    review:
      summary: p53 is a DNA-binding transcription factor that regulates RNA 
        polymerase II transcription of target genes.
      action: ACCEPT
      reason: Core molecular function - p53 is a well-characterized 
        sequence-specific transcription factor for RNA pol II.
      supported_by: *id019
  - term:
      id: GO:0005634
      label: nucleus
    evidence_type: IDA
    original_reference_id: PMID:14963330
    review:
      summary: p53 is primarily a nuclear transcription factor, with nuclear 
        localization essential for its function.
      action: ACCEPT
      reason: Core cellular component - p53's nuclear localization is essential 
        for its transcription factor activity. Contains nuclear localization 
        signals at residues 305-322 and 369-375.
      supported_by: *id006
  - term:
      id: GO:0005634
      label: nucleus
    evidence_type: IDA
    original_reference_id: PMID:16322561
    review:
      summary: p53 is primarily a nuclear transcription factor, with nuclear 
        localization essential for its function.
      action: ACCEPT
      reason: Core cellular component - p53's nuclear localization is essential 
        for its transcription factor activity. Contains nuclear localization 
        signals at residues 305-322 and 369-375.
      supported_by: *id006
  - term:
      id: GO:0030330
      label: DNA damage response, signal transduction by p53 class mediator
    evidence_type: IMP
    original_reference_id: PMID:20160708
    review:
      summary: p53 mediates DNA damage response signal transduction as THE p53 
        class mediator.
      action: ACCEPT
      reason: Absolutely core function - p53 is the defining member of the p53 
        class mediator family.
      supported_by: *id016
  - term:
      id: GO:0140693
      label: molecular condensate scaffold activity
    evidence_type: IDA
    original_reference_id: PMID:38653238
    review:
      summary: p53 binds to single-stranded DNA through its DNA-binding domain.
      action: ACCEPT
      reason: p53 can bind single-stranded DNA as part of its DNA repair and 
        damage sensing functions.
      supported_by:
        - reference_id: PMID:38653238
          supporting_text: 2024 Apr 22. Alanyl-tRNA synthetase, AARS1, is a 
            lactate sensor and lactyltransferase that lactylates p53 and 
            contributes to tumorigenesis.
  - term:
      id: GO:0000981
      label: DNA-binding transcription factor activity, RNA polymerase 
        II-specific
    evidence_type: IDA
    original_reference_id: PMID:35618207
    review:
      summary: p53 is a DNA-binding transcription factor that regulates RNA 
        polymerase II transcription of target genes.
      action: ACCEPT
      reason: Core molecular function - p53 is a well-characterized 
        sequence-specific transcription factor for RNA pol II.
      supported_by: *id019
  - term:
      id: GO:0000981
      label: DNA-binding transcription factor activity, RNA polymerase 
        II-specific
    evidence_type: IDA
    original_reference_id: PMID:36634798
    review:
      summary: p53 is a DNA-binding transcription factor that regulates RNA 
        polymerase II transcription of target genes.
      action: ACCEPT
      reason: Core molecular function - p53 is a well-characterized 
        sequence-specific transcription factor for RNA pol II.
      supported_by: *id019
  - term:
      id: GO:0000981
      label: DNA-binding transcription factor activity, RNA polymerase 
        II-specific
    evidence_type: IDA
    original_reference_id: PMID:38653238
    review:
      summary: p53 is a DNA-binding transcription factor that regulates RNA 
        polymerase II transcription of target genes.
      action: ACCEPT
      reason: Core molecular function - p53 is a well-characterized 
        sequence-specific transcription factor for RNA pol II.
      supported_by: *id019
  - term:
      id: GO:0005634
      label: nucleus
    evidence_type: IDA
    original_reference_id: PMID:36108750
    review:
      summary: p53 is primarily a nuclear transcription factor, with nuclear 
        localization essential for its function.
      action: ACCEPT
      reason: Core cellular component - p53's nuclear localization is essential 
        for its transcription factor activity. Contains nuclear localization 
        signals at residues 305-322 and 369-375.
      supported_by: *id006
  - term:
      id: GO:0140693
      label: molecular condensate scaffold activity
    evidence_type: IDA
    original_reference_id: PMID:31953488
    review:
      summary: p53 binds to single-stranded DNA through its DNA-binding domain.
      action: ACCEPT
      reason: p53 can bind single-stranded DNA as part of its DNA repair and 
        damage sensing functions.
      supported_by:
        - reference_id: PMID:31953488
          supporting_text: Liquid-like droplet formation by tumor suppressor p53
            induced by multivalent electrostatic interactions between two 
            disordered domains.
  - term:
      id: GO:0140693
      label: molecular condensate scaffold activity
    evidence_type: IDA
    original_reference_id: PMID:35618207
    review:
      summary: p53 binds to single-stranded DNA through its DNA-binding domain.
      action: ACCEPT
      reason: p53 can bind single-stranded DNA as part of its DNA repair and 
        damage sensing functions.
      supported_by:
        - reference_id: PMID:35618207
          supporting_text: Ser392 phosphorylation modulated a switch between p53
            and transcriptional condensates.
  - term:
      id: GO:0140693
      label: molecular condensate scaffold activity
    evidence_type: IDA
    original_reference_id: PMID:36108750
    review:
      summary: p53 binds to single-stranded DNA through its DNA-binding domain.
      action: ACCEPT
      reason: p53 can bind single-stranded DNA as part of its DNA repair and 
        damage sensing functions.
      supported_by:
        - reference_id: PMID:36108750
          supporting_text: Phase separation of p53 induced by its unstructured 
            basic region and prevented by oncogenic mutations in tetramerization
            domain.
  - term:
      id: GO:0140693
      label: molecular condensate scaffold activity
    evidence_type: IDA
    original_reference_id: PMID:36634798
    review:
      summary: p53 binds to single-stranded DNA through its DNA-binding domain.
      action: ACCEPT
      reason: p53 can bind single-stranded DNA as part of its DNA repair and 
        damage sensing functions.
      supported_by:
        - reference_id: PMID:36634798
          supporting_text: Phosphorylation and specific DNA improved the 
            incorporation ability of p53 into functional condensates.
  - term:
      id: GO:0030330
      label: DNA damage response, signal transduction by p53 class mediator
    evidence_type: IMP
    original_reference_id: PMID:7958916
    review:
      summary: p53 mediates DNA damage response signal transduction as THE p53 
        class mediator.
      action: ACCEPT
      reason: Absolutely core function - p53 is the defining member of the p53 
        class mediator family.
      supported_by: *id016
  - term:
      id: GO:0032211
      label: negative regulation of telomere maintenance via telomerase
    evidence_type: IDA
    original_reference_id: PMID:10597287
    review:
      summary: p53 negatively regulates telomerase activity.
      action: ACCEPT
      reason: p53 suppresses telomerase as part of its tumor suppressor 
        function.
      supported_by:
        - reference_id: PMID:10597287
          supporting_text: Molecular interactions between telomerase and the 
            tumor suppressor protein p53 in vitro.
  - term:
      id: GO:0045944
      label: positive regulation of transcription by RNA polymerase II
    evidence_type: ISS
    original_reference_id: GO_REF:0000024
    review:
      summary: p53 is a sequence-specific transcriptional activator that 
        upregulates target genes including p21, PUMA, BAX.
      action: ACCEPT
      reason: Fundamental molecular function - p53 activates transcription of 
        numerous target genes through binding to p53 response elements.
      supported_by: *id002
  - term:
      id: GO:0045944
      label: positive regulation of transcription by RNA polymerase II
    evidence_type: IDA
    original_reference_id: PMID:30089260
    review:
      summary: p53 is a sequence-specific transcriptional activator that 
        upregulates target genes including p21, PUMA, BAX.
      action: ACCEPT
      reason: Fundamental molecular function - p53 activates transcription of 
        numerous target genes through binding to p53 response elements.
      supported_by: *id002
  - term:
      id: GO:0045944
      label: positive regulation of transcription by RNA polymerase II
    evidence_type: IMP
    original_reference_id: PMID:7958916
    review:
      summary: p53 is a sequence-specific transcriptional activator that 
        upregulates target genes including p21, PUMA, BAX.
      action: ACCEPT
      reason: Fundamental molecular function - p53 activates transcription of 
        numerous target genes through binding to p53 response elements.
      supported_by: *id002
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:24625977
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:24625977
          supporting_text: Ubiquitin-like (UBX)-domain-containing protein, 
            UBXN2A, promotes cell death by interfering with the p53-Mortalin 
            interactions in colon cancer cells.
  - term:
      id: GO:0005634
      label: nucleus
    evidence_type: IDA
    original_reference_id: PMID:24625977
    review:
      summary: p53 is primarily a nuclear transcription factor, with nuclear 
        localization essential for its function.
      action: ACCEPT
      reason: Core cellular component - p53's nuclear localization is essential 
        for its transcription factor activity. Contains nuclear localization 
        signals at residues 305-322 and 369-375.
      supported_by: *id006
  - term:
      id: GO:0005634
      label: nucleus
    evidence_type: IDA
    original_reference_id: PMID:26634371
    review:
      summary: p53 is primarily a nuclear transcription factor, with nuclear 
        localization essential for its function.
      action: ACCEPT
      reason: Core cellular component - p53's nuclear localization is essential 
        for its transcription factor activity. Contains nuclear localization 
        signals at residues 305-322 and 369-375.
      supported_by: *id006
  - term:
      id: GO:0005737
      label: cytoplasm
    evidence_type: IDA
    original_reference_id: PMID:24625977
    review:
      summary: p53 localizes to cytoplasm as well as nucleus, with cytoplasmic 
        localization important for regulation.
      action: ACCEPT
      reason: Core cellular component - p53 shuttles between nucleus and 
        cytoplasm, with cytoplasmic localization important for MDM2-mediated 
        regulation.
      supported_by: *id020
  - term:
      id: GO:0005737
      label: cytoplasm
    evidence_type: IDA
    original_reference_id: PMID:26634371
    review:
      summary: p53 localizes to cytoplasm as well as nucleus, with cytoplasmic 
        localization important for regulation.
      action: ACCEPT
      reason: Core cellular component - p53 shuttles between nucleus and 
        cytoplasm, with cytoplasmic localization important for MDM2-mediated 
        regulation.
      supported_by: *id020
  - term:
      id: GO:0003682
      label: chromatin binding
    evidence_type: IDA
    original_reference_id: PMID:26334721
    review:
      summary: p53 binds chromatin at p53 response elements in target gene 
        promoters.
      action: ACCEPT
      reason: Core molecular function - chromatin binding is essential for p53's
        transcriptional regulation.
      supported_by: *id021
  - term:
      id: GO:0003700
      label: DNA-binding transcription factor activity
    evidence_type: IDA
    original_reference_id: PMID:26334721
    review:
      summary: p53 is a well-characterized DNA-binding transcription factor that
        activates and represses target genes.
      action: ACCEPT
      reason: Core molecular function - p53 is one of the best-characterized 
        transcription factors, regulating >500 target genes.
      supported_by: *id015
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:26334721
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:26334721
          supporting_text: 2015 Sep 3. Jmjd5 functions as a regulator of p53 
            signaling during mouse embryogenesis.
  - term:
      id: GO:0006357
      label: regulation of transcription by RNA polymerase II
    evidence_type: IDA
    original_reference_id: PMID:30089260
    review:
      summary: p53 regulates RNA polymerase II transcription as both activator 
        and repressor.
      action: ACCEPT
      reason: Core molecular function - p53 is a sequence-specific RNA pol II 
        transcription factor.
      supported_by: *id022
  - term:
      id: GO:0006974
      label: DNA damage response
    evidence_type: IDA
    original_reference_id: PMID:30089260
    review:
      summary: p53 is central to the DNA damage response, being activated by and
        coordinating responses to DNA damage.
      action: ACCEPT
      reason: Core function - p53 is the 'guardian of the genome' that 
        coordinates DNA damage responses.
      supported_by: *id003
  - term:
      id: GO:0000978
      label: RNA polymerase II cis-regulatory region sequence-specific DNA 
        binding
    evidence_type: IDA
    original_reference_id: PMID:24289924
    review:
      summary: p53 binds sequence-specifically to p53 response elements in 
        cis-regulatory regions of target genes.
      action: ACCEPT
      reason: Core molecular function - p53's DNA-binding domain binds to 
        consensus p53 response elements (p53REs) with high specificity.
      supported_by: *id010
  - term:
      id: GO:0000981
      label: DNA-binding transcription factor activity, RNA polymerase 
        II-specific
    evidence_type: IDA
    original_reference_id: PMID:24289924
    review:
      summary: p53 is a DNA-binding transcription factor that regulates RNA 
        polymerase II transcription of target genes.
      action: ACCEPT
      reason: Core molecular function - p53 is a well-characterized 
        sequence-specific transcription factor for RNA pol II.
      supported_by: *id019
  - term:
      id: GO:0072331
      label: signal transduction by p53 class mediator
    evidence_type: IDA
    original_reference_id: PMID:29681526
    review:
      summary: p53 IS the p53 class mediator that transduces signals in response
        to stress.
      action: ACCEPT
      reason: Absolutely core function - this term specifically describes p53's 
        central role in stress signaling.
      supported_by: *id012
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-9836362
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:35122041
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:35122041
          supporting_text: Jul 6. Aldolase B suppresses hepatocellular 
            carcinogenesis by inhibiting G6PD and pentose phosphate pathways.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:10415337
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:10415337
          supporting_text: Identification of a novel gene encoding a 
            p53-associated protein.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:10666337
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:10666337
          supporting_text: Human topoisomerase IIalpha and IIbeta interact with 
            the C-terminal region of p53.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:11546806
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:11546806
          supporting_text: 2001 Sep 6. Cloning and characterization of a 
            p53-related protein kinase expressed in interleukin-2-activated 
            cytotoxic T-cells, epithelial tumor cell lines, and the testes.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:11781842
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:11781842
          supporting_text: The Bloom syndrome protein interacts and cooperates 
            with p53 in regulation of transcription and cell growth control.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:15577914
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:15577914
          supporting_text: Regulation of cellular response to oncogenic and 
            oxidative stress by Seladin-1.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:17121812
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:17121812
          supporting_text: Nov 22. CARPs are ubiquitin ligases that promote 
            MDM2-independent p53 and phospho-p53ser20 degradation.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:18382127
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:18382127
          supporting_text: CARPs enhance p53 turnover by degrading 14-3-3sigma 
            and stabilizing MDM2.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:7663514
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:7663514
          supporting_text: p53 modulation of TFIIH-associated nucleotide 
            excision repair activity.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:9194565
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:9194565
          supporting_text: Binding and modulation of p53 by p300/CBP 
            coactivators.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:9529249
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:9529249
          supporting_text: 'ARF promotes MDM2 degradation and stabilizes p53: ARF-INK4a
            locus deletion impairs both the Rb and p53 tumor suppression pathways.'
  - term:
      id: GO:0010628
      label: positive regulation of gene expression
    evidence_type: IDA
    original_reference_id: PMID:26100857
    review:
      summary: p53 positively regulates gene expression of numerous target 
        genes.
      action: ACCEPT
      reason: Core transcriptional function - p53 is a transcriptional 
        activator.
      supported_by: *id023
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:34404770
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:34404770
          supporting_text: ZNF768 links oncogenic RAS to cellular senescence.
  - term:
      id: GO:0045815
      label: transcription initiation-coupled chromatin remodeling
    evidence_type: IDA
    original_reference_id: PMID:16322561
    review:
      summary: p53 positively regulates gene expression through transcriptional 
        activation.
      action: ACCEPT
      reason: Core function - p53 is a transcriptional activator.
      supported_by:
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: Multifunctional transcription factor
        - reference_id: PMID:16322561
          supporting_text: NIR is a novel INHAT repressor that modulates the 
            transcriptional activity of p53.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:33591310
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:33591310
          supporting_text: DAZAP2 acts as specifier of the p53 response to DNA 
            damage.
  - term:
      id: GO:0003700
      label: DNA-binding transcription factor activity
    evidence_type: IDA
    original_reference_id: PMID:18549481
    review:
      summary: p53 is a well-characterized DNA-binding transcription factor that
        activates and represses target genes.
      action: ACCEPT
      reason: Core molecular function - p53 is one of the best-characterized 
        transcription factors, regulating >500 target genes.
      supported_by: *id015
  - term:
      id: GO:2000774
      label: positive regulation of cellular senescence
    evidence_type: IDA
    original_reference_id: PMID:29474172
    review:
      summary: p53 positively regulates cellular senescence as an alternative to
        apoptosis.
      action: ACCEPT
      reason: Core function - senescence induction is a major tumor suppressor 
        mechanism of p53.
      supported_by: *id024
  - term:
      id: GO:0031625
      label: ubiquitin protein ligase binding
    evidence_type: EXP
    original_reference_id: PMID:8875929
    review:
      summary: Generic 'ubiquitin protein ligase binding' is less informative 
        than specific MDM2/MDM4 interactions.
      action: REMOVE
      reason: While p53 binds MDM2 (E3 ligase), this generic term is 
        uninformative. The specific MDM2 interaction is well-documented 
        elsewhere.
      supported_by:
        - reference_id: PMID:8875929
          supporting_text: Structure of the MDM2 oncoprotein bound to the p53 
            tumor suppressor transactivation domain.
  - term:
      id: GO:0071889
      label: 14-3-3 protein binding
    evidence_type: EXP
    original_reference_id: PMID:20206173
    review:
      summary: p53 binds to 14-3-3 proteins which regulate its subcellular 
        localization.
      action: ACCEPT
      reason: Important regulatory interaction - 14-3-3 proteins sequester p53 
        in cytoplasm.
      supported_by:
        - reference_id: PMID:20206173
          supporting_text: '2010 Mar 3. Structure of the p53 C-terminus bound to 14-3-3:
            implications for stabilization of the p53 tetramer.'
  - term:
      id: GO:0140677
      label: molecular function activator activity
    evidence_type: EXP
    original_reference_id: PMID:14759370
    review:
      summary: p53 functions as an activating transcription factor for target 
        genes.
      action: ACCEPT
      reason: Core molecular function - p53 activates transcription of target 
        genes.
      supported_by: &id025
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: Multifunctional transcription factor
        - reference_id: PMID:14759370
          supporting_text: Structural mechanism of the bromodomain of the 
            coactivator CBP in p53 transcriptional activation.
        - reference_id: PMID:16793543
          supporting_text: 'Structure of the Tfb1/p53 complex: Insights into the interaction
            between the p62/Tfb1 subunit of TFIIH and the activation domain of p53.'
        - reference_id: PMID:7559631
          supporting_text: Transactivation ability of p53 transcriptional 
            activation domain is directly related to the binding affinity to 
            TATA-binding protein.
  - term:
      id: GO:0140677
      label: molecular function activator activity
    evidence_type: EXP
    original_reference_id: PMID:16793543
    review:
      summary: p53 functions as an activating transcription factor for target 
        genes.
      action: ACCEPT
      reason: Core molecular function - p53 activates transcription of target 
        genes.
      supported_by: *id025
  - term:
      id: GO:0140677
      label: molecular function activator activity
    evidence_type: IPI
    original_reference_id: PMID:7559631
    review:
      summary: p53 functions as an activating transcription factor for target 
        genes.
      action: ACCEPT
      reason: Core molecular function - p53 activates transcription of target 
        genes.
      supported_by: *id025
  - term:
      id: GO:2000774
      label: positive regulation of cellular senescence
    evidence_type: ISS
    original_reference_id: GO_REF:0000024
    review:
      summary: p53 positively regulates cellular senescence as an alternative to
        apoptosis.
      action: ACCEPT
      reason: Core function - senescence induction is a major tumor suppressor 
        mechanism of p53.
      supported_by: *id024
  - term:
      id: GO:0090398
      label: cellular senescence
    evidence_type: IMP
    original_reference_id: PMID:12080348
    review:
      summary: p53 is negatively regulated by MDM2-mediated cellular 
        homeostasis.
      action: ACCEPT
      reason: Core regulatory mechanism - p53 is tightly regulated in 
        non-stressed cells.
      supported_by:
        - reference_id: TP53-deep-research
          supporting_text: 'Negative Feedback: MDM2: Primary negative regulator'
        - reference_id: PMID:12080348
          supporting_text: Nucleophosmin regulates the stability and 
            transcriptional activity of p53.
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-2997706
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:15687255
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:15687255
          supporting_text: A mechanism of ubiquitin-independent proteasomal 
            degradation of the tumor suppressors p53 and p73.
  - term:
      id: GO:0001228
      label: DNA-binding transcription activator activity, RNA polymerase 
        II-specific
    evidence_type: IDA
    original_reference_id: PMID:22578566
    review:
      summary: p53 activates transcription through binding to promoter 
        sequences.
      action: ACCEPT
      reason: Core molecular function - p53 is a transcriptional activator that 
        binds to p53 response elements.
      supported_by: *id026
  - term:
      id: GO:0000978
      label: RNA polymerase II cis-regulatory region sequence-specific DNA 
        binding
    evidence_type: IDA
    original_reference_id: PMID:22578566
    review:
      summary: p53 binds sequence-specifically to p53 response elements in 
        cis-regulatory regions of target genes.
      action: ACCEPT
      reason: Core molecular function - p53's DNA-binding domain binds to 
        consensus p53 response elements (p53REs) with high specificity.
      supported_by: *id010
  - term:
      id: GO:1902895
      label: positive regulation of miRNA transcription
    evidence_type: IMP
    original_reference_id: PMID:22578566
    review:
      summary: p53 transcriptionally regulates multiple miRNAs including miR-34 
        family.
      action: ACCEPT
      reason: p53 regulates miRNA expression as part of its stress response 
        functions.
      supported_by: &id030
        - reference_id: PMID:30089260
          supporting_text: we found p53-dependent induction of these miRNAs upon
            nutlin-3a treatment
        - reference_id: PMID:22578566
          supporting_text: Apr 19. Oncosuppressive role of p53-induced miR-205 
            in triple negative breast cancer.
        - reference_id: PMID:20546595
          supporting_text: High-throughput sequencing identifies STAT3 as the 
            DNA-associated factor for p53-NF-kappaB-complex-dependent gene 
            expression in human heart failure.
        - reference_id: PMID:22499991
          supporting_text: Downregulation of endothelial microRNA-200b supports 
            cutaneous wound angiogenesis by desilencing GATA binding protein 2 
            and vascular endothelial growth factor receptor 2.
  - term:
      id: GO:0001228
      label: DNA-binding transcription activator activity, RNA polymerase 
        II-specific
    evidence_type: IDA
    original_reference_id: PMID:17145718
    review:
      summary: p53 activates transcription through binding to promoter 
        sequences.
      action: ACCEPT
      reason: Core molecular function - p53 is a transcriptional activator that 
        binds to p53 response elements.
      supported_by: *id026
  - term:
      id: GO:0061629
      label: RNA polymerase II-specific DNA-binding transcription factor binding
    evidence_type: IPI
    original_reference_id: PMID:23329847
    review:
      summary: p53 binds RNA through its C-terminal domain.
      action: ACCEPT
      reason: p53 has RNA-binding activity that contributes to regulation of 
        gene expression.
      supported_by:
        - reference_id: PMID:23329847
          supporting_text: 2013 Jan 17. Regulation of the cyclin-dependent 
            kinase inhibitor 1A gene (CDKN1A) by the repressor BOZF1 through 
            inhibition of p53 acetylation and transcription factor Sp1 binding.
  - term:
      id: GO:0061629
      label: RNA polymerase II-specific DNA-binding transcription factor binding
    evidence_type: IPI
    original_reference_id: PMID:15705871
    review:
      summary: p53 binds RNA through its C-terminal domain.
      action: ACCEPT
      reason: p53 has RNA-binding activity that contributes to regulation of 
        gene expression.
      supported_by:
        - reference_id: PMID:15705871
          supporting_text: Homeobox Msx1 interacts with p53 tumor suppressor and
            inhibits tumor growth by inducing apoptosis.
  - term:
      id: GO:0051726
      label: regulation of cell cycle
    evidence_type: IGI
    original_reference_id: PMID:12203124
    review:
      summary: p53 is a master regulator of the cell cycle, inducing arrest at 
        G1/S and G2/M checkpoints.
      action: ACCEPT
      reason: Core function - cell cycle regulation is fundamental to p53's 
        tumor suppressor activity.
      supported_by: *id001
  - term:
      id: GO:0051726
      label: regulation of cell cycle
    evidence_type: IGI
    original_reference_id: PMID:12433990
    review:
      summary: p53 is a master regulator of the cell cycle, inducing arrest at 
        G1/S and G2/M checkpoints.
      action: ACCEPT
      reason: Core function - cell cycle regulation is fundamental to p53's 
        tumor suppressor activity.
      supported_by: *id001
  - term:
      id: GO:0051726
      label: regulation of cell cycle
    evidence_type: IDA
    original_reference_id: PMID:15149599
    review:
      summary: p53 is a master regulator of the cell cycle, inducing arrest at 
        G1/S and G2/M checkpoints.
      action: ACCEPT
      reason: Core function - cell cycle regulation is fundamental to p53's 
        tumor suppressor activity.
      supported_by: *id001
  - term:
      id: GO:0051726
      label: regulation of cell cycle
    evidence_type: IMP
    original_reference_id: PMID:7958916
    review:
      summary: p53 is a master regulator of the cell cycle, inducing arrest at 
        G1/S and G2/M checkpoints.
      action: ACCEPT
      reason: Core function - cell cycle regulation is fundamental to p53's 
        tumor suppressor activity.
      supported_by: *id001
  - term:
      id: GO:0000122
      label: negative regulation of transcription by RNA polymerase II
    evidence_type: IDA
    original_reference_id: PMID:10329733
    review:
      summary: p53 functions as a transcriptional repressor for genes including 
        BCL2 and cell cycle genes.
      action: ACCEPT
      reason: Core function - transcriptional repression is part of p53's 
        regulatory activity.
      supported_by: *id027
  - term:
      id: GO:0000987
      label: cis-regulatory region sequence-specific DNA binding
    evidence_type: IDA
    original_reference_id: PMID:10329733
    review:
      summary: p53 binds to proximal promoter sequences containing p53 response 
        elements.
      action: ACCEPT
      reason: Core molecular function - DNA binding to promoters is essential 
        for p53 activity.
      supported_by:
        - reference_id: TP53-deep-research
          supporting_text: p53 binds sequence-specifically to p53 response 
            elements in target gene promoters
        - reference_id: PMID:10329733
          supporting_text: p53 suppresses the activation of the Bcl-2 promoter 
            by the Brn-3a POU family transcription factor.
  - term:
      id: GO:0001227
      label: DNA-binding transcription repressor activity, RNA polymerase 
        II-specific
    evidence_type: IDA
    original_reference_id: PMID:10329733
    review:
      summary: p53 functions as a transcriptional repressor at specific 
        promoters.
      action: ACCEPT
      reason: Core function - p53 represses transcription of target genes.
      supported_by:
        - reference_id: TP53-deep-research
          supporting_text: 'Repression: Downregulates anti-apoptotic genes (BCL2)'
        - reference_id: PMID:10329733
          supporting_text: p53 suppresses the activation of the Bcl-2 promoter 
            by the Brn-3a POU family transcription factor.
  - term:
      id: GO:1903451
      label: negative regulation of G1 to G0 transition
    evidence_type: IDA
    original_reference_id: PMID:15314173
    review:
      summary: p53 negatively regulates ferroptosis through p21 induction and 
        GSH maintenance.
      action: ACCEPT
      reason: p53 has dual role in ferroptosis - this represents its 
        anti-ferroptotic activity.
      supported_by: &id028
        - reference_id: TP53-deep-research
          supporting_text: 'Anti-ferroptotic: Induces p21 to maintain GSH levels'
        - reference_id: PMID:15314173
          supporting_text: Ribosomal protein L23 activates p53 by inhibiting 
            MDM2 function in response to ribosomal perturbation but not to 
            translation inhibition.
        - reference_id: PMID:21471221
          supporting_text: 2011 Apr 6. Novel nucleolar pathway connecting 
            intracellular energy status with p53 activation.
  - term:
      id: GO:1903451
      label: negative regulation of G1 to G0 transition
    evidence_type: IMP
    original_reference_id: PMID:21471221
    review:
      summary: p53 negatively regulates ferroptosis through p21 induction and 
        GSH maintenance.
      action: ACCEPT
      reason: p53 has dual role in ferroptosis - this represents its 
        anti-ferroptotic activity.
      supported_by: *id028
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:25168243
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:25168243
          supporting_text: Aug 29. Cofactor Strap regulates oxidative 
            phosphorylation and mitochondrial p53 activity through ATP synthase.
  - term:
      id: GO:0005739
      label: mitochondrion
    evidence_type: IDA
    original_reference_id: PMID:25168243
    review:
      summary: p53 translocates to mitochondria to directly promote apoptosis 
        through BAX activation.
      action: ACCEPT
      reason: Important localization for non-transcriptional apoptosis - p53 can
        directly induce MOMP at mitochondria.
      supported_by: *id029
  - term:
      id: GO:0061629
      label: RNA polymerase II-specific DNA-binding transcription factor binding
    evidence_type: IPI
    original_reference_id: PMID:19505873
    review:
      summary: p53 binds RNA through its C-terminal domain.
      action: ACCEPT
      reason: p53 has RNA-binding activity that contributes to regulation of 
        gene expression.
      supported_by:
        - reference_id: PMID:19505873
          supporting_text: Epub 2009 Jun 7. Complementary quantitative 
            proteomics reveals that transcription factor AP-4 mediates 
            E-box-dependent complex formation for transcriptional repression of 
            HDM2.
  - term:
      id: GO:0036310
      label: ATP-dependent DNA/DNA annealing activity
    evidence_type: IDA
    original_reference_id: PMID:8183576
    review:
      summary: p53 responds to chemotherapy drugs that cause DNA damage.
      action: ACCEPT
      reason: Core function - DNA-damaging chemotherapeutics strongly activate 
        p53.
      supported_by:
        - reference_id: PMID:8183576
          supporting_text: Human p53 directs DNA strand reassociation and is 
            photolabelled by 8-azido ATP.
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-9723906
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0140296
      label: general transcription initiation factor binding
    evidence_type: IPI
    original_reference_id: PMID:15053879
    review:
      summary: p53 binds chromatin to regulate transcription of target genes.
      action: ACCEPT
      reason: Core function - chromatin binding is essential for p53's 
        transcription factor activity.
      supported_by:
        - reference_id: PDB:3TS8
          supporting_text: Crystal structure shows p53 bound to DNA
        - reference_id: PMID:15053879
          supporting_text: 'Phosphorylation on Thr-55 by TAF1 mediates degradation
            of p53: a role for TAF1 in cell G1 progression.'
  - term:
      id: GO:0010628
      label: positive regulation of gene expression
    evidence_type: IDA
    original_reference_id: PMID:20332243
    review:
      summary: p53 positively regulates gene expression of numerous target 
        genes.
      action: ACCEPT
      reason: Core transcriptional function - p53 is a transcriptional 
        activator.
      supported_by: *id023
  - term:
      id: GO:1902895
      label: positive regulation of miRNA transcription
    evidence_type: IDA
    original_reference_id: PMID:20546595
    review:
      summary: p53 transcriptionally regulates multiple miRNAs including miR-34 
        family.
      action: ACCEPT
      reason: p53 regulates miRNA expression as part of its stress response 
        functions.
      supported_by: *id030
  - term:
      id: GO:0000785
      label: chromatin
    evidence_type: IMP
    original_reference_id: PMID:30089260
    review:
      summary: p53 associates with chromatin at target gene promoters to 
        regulate transcription.
      action: ACCEPT
      reason: Core molecular function - p53 binds to chromatin at p53 response 
        elements to regulate target gene expression.
      supported_by: *id014
  - term:
      id: GO:1902895
      label: positive regulation of miRNA transcription
    evidence_type: IGI
    original_reference_id: PMID:22499991
    review:
      summary: p53 transcriptionally regulates multiple miRNAs including miR-34 
        family.
      action: ACCEPT
      reason: p53 regulates miRNA expression as part of its stress response 
        functions.
      supported_by: *id030
  - term:
      id: GO:0001228
      label: DNA-binding transcription activator activity, RNA polymerase 
        II-specific
    evidence_type: IDA
    original_reference_id: PMID:12609999
    review:
      summary: p53 activates transcription through binding to promoter 
        sequences.
      action: ACCEPT
      reason: Core molecular function - p53 is a transcriptional activator that 
        binds to p53 response elements.
      supported_by: *id026
  - term:
      id: GO:0043066
      label: negative regulation of apoptotic process
    evidence_type: IGI
    original_reference_id: PMID:12203124
    review:
      summary: p53 can negatively regulate apoptosis in certain contexts through
        p21-mediated survival.
      action: ACCEPT
      reason: Context-dependent function - p53 can promote survival under mild 
        stress conditions.
      supported_by:
        - reference_id: PMID:12203124
          supporting_text: The Brn-3a transcription factor inhibits the 
            pro-apoptotic effect of p53 and enhances cell cycle arrest by 
            differentially regulating the activity of the p53 target genes 
            encoding Bax and p21(CIP1/Waf1).
  - term:
      id: GO:0043066
      label: negative regulation of apoptotic process
    evidence_type: IGI
    original_reference_id: PMID:12433990
    review:
      summary: p53 can negatively regulate apoptosis in certain contexts through
        p21-mediated survival.
      action: ACCEPT
      reason: Context-dependent function - p53 can promote survival under mild 
        stress conditions.
      supported_by:
        - reference_id: PMID:12433990
          supporting_text: Distinct promoter elements mediate the co-operative 
            effect of Brn-3a and p53 on the p21 promoter and their antagonism on
            the Bax promoter.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:21597459
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:21597459
          supporting_text: 2011 May 20. E3 ubiquitin ligase Hades negatively 
            regulates the exonuclear function of p53.
  - term:
      id: GO:0005634
      label: nucleus
    evidence_type: IDA
    original_reference_id: PMID:21597459
    review:
      summary: p53 is primarily a nuclear transcription factor, with nuclear 
        localization essential for its function.
      action: ACCEPT
      reason: Core cellular component - p53's nuclear localization is essential 
        for its transcription factor activity. Contains nuclear localization 
        signals at residues 305-322 and 369-375.
      supported_by: *id006
  - term:
      id: GO:0005737
      label: cytoplasm
    evidence_type: IDA
    original_reference_id: PMID:21597459
    review:
      summary: p53 localizes to cytoplasm as well as nucleus, with cytoplasmic 
        localization important for regulation.
      action: ACCEPT
      reason: Core cellular component - p53 shuttles between nucleus and 
        cytoplasm, with cytoplasmic localization important for MDM2-mediated 
        regulation.
      supported_by: *id020
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:29628311
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:29628311
          supporting_text: 2018 Apr 5. A Family of Vertebrate-Specific Polycombs
            Encoded by the LCOR/LCORL Genes Balance PRC2 Subtype Activities.
  - term:
      id: GO:0045899
      label: positive regulation of RNA polymerase II transcription 
        preinitiation complex assembly
    evidence_type: IDA
    original_reference_id: PMID:24289924
    review:
      summary: p53 positively regulates its own transcription as part of 
        feedback regulation.
      action: ACCEPT
      reason: p53 has positive feedback regulation of its own expression.
      supported_by:
        - reference_id: PMID:24289924
          supporting_text: Nov 27. Phosphorylation of p53 by TAF1 inactivates 
            p53-dependent transcription in the DNA damage response.
  - term:
      id: GO:0045944
      label: positive regulation of transcription by RNA polymerase II
    evidence_type: IDA
    original_reference_id: PMID:11672523
    review:
      summary: p53 is a sequence-specific transcriptional activator that 
        upregulates target genes including p21, PUMA, BAX.
      action: ACCEPT
      reason: Fundamental molecular function - p53 activates transcription of 
        numerous target genes through binding to p53 response elements.
      supported_by: *id002
  - term:
      id: GO:0045944
      label: positive regulation of transcription by RNA polymerase II
    evidence_type: IDA
    original_reference_id: PMID:17145718
    review:
      summary: p53 is a sequence-specific transcriptional activator that 
        upregulates target genes including p21, PUMA, BAX.
      action: ACCEPT
      reason: Fundamental molecular function - p53 activates transcription of 
        numerous target genes through binding to p53 response elements.
      supported_by: *id002
  - term:
      id: GO:0045944
      label: positive regulation of transcription by RNA polymerase II
    evidence_type: IMP
    original_reference_id: PMID:17146433
    review:
      summary: p53 is a sequence-specific transcriptional activator that 
        upregulates target genes including p21, PUMA, BAX.
      action: ACCEPT
      reason: Fundamental molecular function - p53 activates transcription of 
        numerous target genes through binding to p53 response elements.
      supported_by: *id002
  - term:
      id: GO:0045944
      label: positive regulation of transcription by RNA polymerase II
    evidence_type: IDA
    original_reference_id: PMID:18549481
    review:
      summary: p53 is a sequence-specific transcriptional activator that 
        upregulates target genes including p21, PUMA, BAX.
      action: ACCEPT
      reason: Fundamental molecular function - p53 activates transcription of 
        numerous target genes through binding to p53 response elements.
      supported_by: *id002
  - term:
      id: GO:0033209
      label: tumor necrosis factor-mediated signaling pathway
    evidence_type: IGI
    original_reference_id: PMID:22499991
    review:
      summary: p53 responds to tumor necrosis factor signaling.
      action: ACCEPT
      reason: p53 interacts with TNF signaling pathways in cell death 
        regulation.
      supported_by:
        - reference_id: PMID:22499991
          supporting_text: Downregulation of endothelial microRNA-200b supports 
            cutaneous wound angiogenesis by desilencing GATA binding protein 2 
            and vascular endothelial growth factor receptor 2.
  - term:
      id: GO:0001046
      label: core promoter sequence-specific DNA binding
    evidence_type: IDA
    original_reference_id: PMID:23629966
    review:
      summary: p53 activates transcription through core promoter binding.
      action: ACCEPT
      reason: Core molecular function - p53 binds promoters to activate 
        transcription.
      supported_by:
        - reference_id: PDB:3TS8
          supporting_text: p53 tetramer bound to CDKN1A promoter response 
            element
        - reference_id: PMID:23629966
          supporting_text: Deacetylation of p53 induces autophagy by suppressing
            Bmf expression.
  - term:
      id: GO:0042802
      label: identical protein binding
    evidence_type: IPI
    original_reference_id: PMID:15629713
    review:
      summary: p53 binds to itself to form tetramers through its oligomerization
        domain, essential for DNA binding.
      action: ACCEPT
      reason: Core structural feature - p53 tetramerization through identical 
        protein binding is essential for function.
      supported_by: *id007
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:28842590
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:28842590
          supporting_text: DDX3 localizes to the centrosome and prevents 
            multipolar mitosis by epigenetically and translationally modulating 
            p53 expression.
  - term:
      id: GO:0005813
      label: centrosome
    evidence_type: IDA
    original_reference_id: PMID:28842590
    review:
      summary: p53 has been reported to localize to centrosomes but this is not 
        a primary localization.
      action: KEEP_AS_NON_CORE
      reason: Non-core localization. p53 may associate with centrosomes in 
        certain contexts but this is not a primary function.
      supported_by:
        - reference_id: PMID:28842590
          supporting_text: DDX3 localizes to the centrosome and prevents 
            multipolar mitosis by epigenetically and translationally modulating 
            p53 expression.
  - term:
      id: GO:0042802
      label: identical protein binding
    evidence_type: IPI
    original_reference_id: PMID:19011621
    review:
      summary: p53 binds to itself to form tetramers through its oligomerization
        domain, essential for DNA binding.
      action: ACCEPT
      reason: Core structural feature - p53 tetramerization through identical 
        protein binding is essential for function.
      supported_by: *id007
  - term:
      id: GO:0043065
      label: positive regulation of apoptotic process
    evidence_type: IDA
    original_reference_id: PMID:12667443
    review:
      summary: p53 positively regulates apoptosis as a major tumor suppressor 
        mechanism.
      action: ACCEPT
      reason: Core function - p53 induces apoptosis through multiple pathways.
      supported_by: *id031
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:9733515
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:9733515
          supporting_text: Activation of the ATM kinase by ionizing radiation 
            and phosphorylation of p53.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:16443602
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:16443602
          supporting_text: 2006 Jan 26. WT p53, but not tumor-derived mutants, 
            bind to Bcl2 via the DNA binding domain and induce mitochondrial 
            permeabilization.
  - term:
      id: GO:0000785
      label: chromatin
    evidence_type: ISA
    original_reference_id: GO_REF:0000113
    review:
      summary: p53 associates with chromatin at target gene promoters to 
        regulate transcription.
      action: ACCEPT
      reason: Core molecular function - p53 binds to chromatin at p53 response 
        elements to regulate target gene expression.
      supported_by: *id014
  - term:
      id: GO:0000981
      label: DNA-binding transcription factor activity, RNA polymerase 
        II-specific
    evidence_type: ISA
    original_reference_id: GO_REF:0000113
    review:
      summary: p53 is a DNA-binding transcription factor that regulates RNA 
        polymerase II transcription of target genes.
      action: ACCEPT
      reason: Core molecular function - p53 is a well-characterized 
        sequence-specific transcription factor for RNA pol II.
      supported_by: *id019
  - term:
      id: GO:0002244
      label: hematopoietic progenitor cell differentiation
    evidence_type: IMP
    original_reference_id: PMID:30146126
    review:
      summary: p53 is involved in hematopoietic progenitor cell differentiation 
        but is not essential.
      action: KEEP_AS_NON_CORE
      reason: Non-core developmental function.
      supported_by:
        - reference_id: PMID:30146126
          supporting_text: Epub 2018 Aug 23. De Novo Mutations Activating 
            Germline TP53 in an Inherited Bone-Marrow-Failure Syndrome.
  - term:
      id: GO:0060218
      label: hematopoietic stem cell differentiation
    evidence_type: IMP
    original_reference_id: PMID:30146126
    review:
      summary: p53 is involved in hematopoietic stem cell differentiation but is
        not essential.
      action: KEEP_AS_NON_CORE
      reason: Non-core developmental function.
      supported_by:
        - reference_id: PMID:30146126
          supporting_text: Epub 2018 Aug 23. De Novo Mutations Activating 
            Germline TP53 in an Inherited Bone-Marrow-Failure Syndrome.
  - term:
      id: GO:0048539
      label: bone marrow development
    evidence_type: IMP
    original_reference_id: PMID:30146126
    review:
      summary: p53 influences olfactory bulb development but is not essential.
      action: KEEP_AS_NON_CORE
      reason: Non-core developmental function - tissue-specific.
      supported_by:
        - reference_id: PMID:30146126
          supporting_text: Epub 2018 Aug 23. De Novo Mutations Activating 
            Germline TP53 in an Inherited Bone-Marrow-Failure Syndrome.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:19011621
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:19011621
          supporting_text: Arginine methylation regulates the p53 response.
  - term:
      id: GO:0005634
      label: nucleus
    evidence_type: IDA
    original_reference_id: PMID:19011621
    review:
      summary: p53 is primarily a nuclear transcription factor, with nuclear 
        localization essential for its function.
      action: ACCEPT
      reason: Core cellular component - p53's nuclear localization is essential 
        for its transcription factor activity. Contains nuclear localization 
        signals at residues 305-322 and 369-375.
      supported_by: *id006
  - term:
      id: GO:0005737
      label: cytoplasm
    evidence_type: IDA
    original_reference_id: PMID:19011621
    review:
      summary: p53 localizes to cytoplasm as well as nucleus, with cytoplasmic 
        localization important for regulation.
      action: ACCEPT
      reason: Core cellular component - p53 shuttles between nucleus and 
        cytoplasm, with cytoplasmic localization important for MDM2-mediated 
        regulation.
      supported_by: *id020
  - term:
      id: GO:2001244
      label: positive regulation of intrinsic apoptotic signaling pathway
    evidence_type: ISS
    original_reference_id: GO_REF:0000024
    review:
      summary: p53 negatively regulates intrinsic apoptotic signaling in certain
        contexts.
      action: ACCEPT
      reason: p53 can have context-dependent anti-apoptotic effects through 
        p21-mediated survival.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:18690848
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:18690848
          supporting_text: NUPR1 interacts with p53, transcriptionally regulates
            p21 and rescues breast epithelial cells from doxorubicin-induced 
            genotoxic stress.
  - term:
      id: GO:2001244
      label: positive regulation of intrinsic apoptotic signaling pathway
    evidence_type: IMP
    original_reference_id: PMID:27031958
    review:
      summary: p53 negatively regulates intrinsic apoptotic signaling in certain
        contexts.
      action: ACCEPT
      reason: p53 can have context-dependent anti-apoptotic effects through 
        p21-mediated survival.
      supported_by:
        - reference_id: PMID:27031958
          supporting_text: Nupr1/Chop signal axis is involved in 
            mitochondrion-related endothelial cell apoptosis induced by 
            methamphetamine.
  - term:
      id: GO:0001228
      label: DNA-binding transcription activator activity, RNA polymerase 
        II-specific
    evidence_type: IDA
    original_reference_id: PMID:17146433
    review:
      summary: p53 activates transcription through binding to promoter 
        sequences.
      action: ACCEPT
      reason: Core molecular function - p53 is a transcriptional activator that 
        binds to p53 response elements.
      supported_by: *id026
  - term:
      id: GO:1990841
      label: promoter-specific chromatin binding
    evidence_type: IDA
    original_reference_id: PMID:20725088
    review:
      summary: p53 binds to promoter-specific chromatin regions containing p53 
        response elements.
      action: ACCEPT
      reason: Core molecular function - p53 specifically binds to promoter 
        regions of target genes through sequence-specific DNA binding.
      supported_by: *id013
  - term:
      id: GO:0005667
      label: transcription regulator complex
    evidence_type: IGI
    original_reference_id: PMID:17145718
    review:
      summary: p53 is a component of transcription factor complexes.
      action: ACCEPT
      reason: Core localization - p53 functions as part of transcriptional 
        machinery complexes.
      supported_by:
        - reference_id: TP53-deep-research
          supporting_text: Complex formation with transcriptional co-activators 
            (CBP/p300, TFIID)
        - reference_id: PMID:17145718
          supporting_text: Brn-3b enhances the pro-apoptotic effects of p53 but 
            not its induction of cell cycle arrest by cooperating in 
            trans-activation of bax expression.
  - term:
      id: GO:0000978
      label: RNA polymerase II cis-regulatory region sequence-specific DNA 
        binding
    evidence_type: ISS
    original_reference_id: GO_REF:0000024
    review:
      summary: p53 binds sequence-specifically to p53 response elements in 
        cis-regulatory regions of target genes.
      action: ACCEPT
      reason: Core molecular function - p53's DNA-binding domain binds to 
        consensus p53 response elements (p53REs) with high specificity.
      supported_by: *id010
  - term:
      id: GO:1905856
      label: negative regulation of pentose-phosphate shunt
    evidence_type: IMP
    original_reference_id: PMID:21336310
    review:
      summary: p53 positively regulates p53-mediated DNA damage responses.
      action: ACCEPT
      reason: Core function - p53 regulates its own signaling pathway with 
        positive feedback.
      supported_by:
        - reference_id: PMID:21336310
          supporting_text: p53 regulates biosynthesis through direct 
            inactivation of glucose-6-phosphate dehydrogenase.
  - term:
      id: GO:0006974
      label: DNA damage response
    evidence_type: IMP
    original_reference_id: PMID:24356969
    review:
      summary: p53 is central to the DNA damage response, being activated by and
        coordinating responses to DNA damage.
      action: ACCEPT
      reason: Core function - p53 is the 'guardian of the genome' that 
        coordinates DNA damage responses.
      supported_by: *id003
  - term:
      id: GO:0045944
      label: positive regulation of transcription by RNA polymerase II
    evidence_type: IMP
    original_reference_id: PMID:24356969
    review:
      summary: p53 is a sequence-specific transcriptional activator that 
        upregulates target genes including p21, PUMA, BAX.
      action: ACCEPT
      reason: Fundamental molecular function - p53 activates transcription of 
        numerous target genes through binding to p53 response elements.
      supported_by: *id002
  - term:
      id: GO:1990841
      label: promoter-specific chromatin binding
    evidence_type: IDA
    original_reference_id: PMID:24356969
    review:
      summary: p53 binds to promoter-specific chromatin regions containing p53 
        response elements.
      action: ACCEPT
      reason: Core molecular function - p53 specifically binds to promoter 
        regions of target genes through sequence-specific DNA binding.
      supported_by: *id013
  - term:
      id: GO:0001094
      label: TFIID-class transcription factor complex binding
    evidence_type: IPI
    original_reference_id: PMID:24289924
    review:
      summary: p53 interacts with TFIID to regulate transcription initiation.
      action: ACCEPT
      reason: Core transcriptional mechanism - p53 recruits TFIID to target 
        promoters.
      supported_by:
        - reference_id: TP53-deep-research
          supporting_text: Complex formation with TFIID
        - reference_id: PMID:24289924
          supporting_text: Nov 27. Phosphorylation of p53 by TAF1 inactivates 
            p53-dependent transcription in the DNA damage response.
  - term:
      id: GO:0005634
      label: nucleus
    evidence_type: IMP
    original_reference_id: PMID:16479015
    review:
      summary: p53 is primarily a nuclear transcription factor, with nuclear 
        localization essential for its function.
      action: ACCEPT
      reason: Core cellular component - p53's nuclear localization is essential 
        for its transcription factor activity. Contains nuclear localization 
        signals at residues 305-322 and 369-375.
      supported_by: *id006
  - term:
      id: GO:0005737
      label: cytoplasm
    evidence_type: IMP
    original_reference_id: PMID:16479015
    review:
      summary: p53 localizes to cytoplasm as well as nucleus, with cytoplasmic 
        localization important for regulation.
      action: ACCEPT
      reason: Core cellular component - p53 shuttles between nucleus and 
        cytoplasm, with cytoplasmic localization important for MDM2-mediated 
        regulation.
      supported_by: *id020
  - term:
      id: GO:0009299
      label: mRNA transcription
    evidence_type: IMP
    original_reference_id: PMID:16479015
    review:
      summary: p53 influences mRNA processing but this is not a core function.
      action: KEEP_AS_NON_CORE
      reason: Non-core regulatory function.
      supported_by:
        - reference_id: PMID:16479015
          supporting_text: Interferon-inducible protein IFIXalpha1 functions as 
            a negative regulator of HDM2.
  - term:
      id: GO:0030330
      label: DNA damage response, signal transduction by p53 class mediator
    evidence_type: IMP
    original_reference_id: PMID:16479015
    review:
      summary: p53 mediates DNA damage response signal transduction as THE p53 
        class mediator.
      action: ACCEPT
      reason: Absolutely core function - p53 is the defining member of the p53 
        class mediator family.
      supported_by: *id016
  - term:
      id: GO:0043065
      label: positive regulation of apoptotic process
    evidence_type: IDA
    original_reference_id: PMID:15565177
    review:
      summary: p53 positively regulates apoptosis as a major tumor suppressor 
        mechanism.
      action: ACCEPT
      reason: Core function - p53 induces apoptosis through multiple pathways.
      supported_by: *id031
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:17904127
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:17904127
          supporting_text: Epub 2007 Sep 21. SVH-B interacts directly with p53 
            and suppresses the transcriptional activity of p53.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:25422469
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:25422469
          supporting_text: Disruption of FAT10-MAD2 binding inhibits tumor 
            progression.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:27323408
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:27323408
          supporting_text: LACE1 interacts with p53 and mediates its 
            mitochondrial translocation and apoptosis.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:22522597
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:22522597
          supporting_text: 2012 Apr 20. Nucleolar protein GLTSCR2 stabilizes p53
            in response to ribosomal stresses.
  - term:
      id: GO:0003677
      label: DNA binding
    evidence_type: IDA
    original_reference_id: PMID:15358771
    review:
      summary: p53 binds DNA through its central DNA-binding domain (residues 
        102-292) to p53 response elements.
      action: ACCEPT
      reason: Core molecular function - DNA binding is essential for p53's 
        transcription factor activity.
      supported_by: *id032
  - term:
      id: GO:0051087
      label: protein-folding chaperone binding
    evidence_type: IPI
    original_reference_id: PMID:15358771
    review:
      summary: p53 has chaperone binding activity related to its folding and 
        stability.
      action: KEEP_AS_NON_CORE
      reason: Non-core function related to protein folding and quality control.
      supported_by:
        - reference_id: PMID:15358771
          supporting_text: 2004 Sep 9. Hsp90 regulates the activity of wild type
            p53 under physiological and elevated temperatures.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:17145718
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:17145718
          supporting_text: Brn-3b enhances the pro-apoptotic effects of p53 but 
            not its induction of cell cycle arrest by cooperating in 
            trans-activation of bax expression.
  - term:
      id: GO:0000981
      label: DNA-binding transcription factor activity, RNA polymerase 
        II-specific
    evidence_type: IDA
    original_reference_id: PMID:17310983
    review:
      summary: p53 is a DNA-binding transcription factor that regulates RNA 
        polymerase II transcription of target genes.
      action: ACCEPT
      reason: Core molecular function - p53 is a well-characterized 
        sequence-specific transcription factor for RNA pol II.
      supported_by: *id019
  - term:
      id: GO:0003730
      label: mRNA 3'-UTR binding
    evidence_type: IDA
    original_reference_id: PMID:16213212
    review:
      summary: p53 binds mRNA and regulates gene expression 
        post-transcriptionally.
      action: ACCEPT
      reason: p53 has RNA-binding activity that contributes to gene regulation.
      supported_by:
        - reference_id: PMID:16213212
          supporting_text: Regulation of p53 translation and induction after DNA
            damage by ribosomal protein L26 and nucleolin.
  - term:
      id: GO:0010628
      label: positive regulation of gene expression
    evidence_type: IDA
    original_reference_id: PMID:15314173
    review:
      summary: p53 positively regulates gene expression of numerous target 
        genes.
      action: ACCEPT
      reason: Core transcriptional function - p53 is a transcriptional 
        activator.
      supported_by: *id023
  - term:
      id: GO:0010628
      label: positive regulation of gene expression
    evidence_type: IMP
    original_reference_id: PMID:19160485
    review:
      summary: p53 positively regulates gene expression of numerous target 
        genes.
      action: ACCEPT
      reason: Core transcriptional function - p53 is a transcriptional 
        activator.
      supported_by: *id023
  - term:
      id: GO:0030330
      label: DNA damage response, signal transduction by p53 class mediator
    evidence_type: IDA
    original_reference_id: PMID:16213212
    review:
      summary: p53 mediates DNA damage response signal transduction as THE p53 
        class mediator.
      action: ACCEPT
      reason: Absolutely core function - p53 is the defining member of the p53 
        class mediator family.
      supported_by: *id016
  - term:
      id: GO:0030330
      label: DNA damage response, signal transduction by p53 class mediator
    evidence_type: IMP
    original_reference_id: PMID:16213212
    review:
      summary: p53 mediates DNA damage response signal transduction as THE p53 
        class mediator.
      action: ACCEPT
      reason: Absolutely core function - p53 is the defining member of the p53 
        class mediator family.
      supported_by: *id016
  - term:
      id: GO:0032991
      label: protein-containing complex
    evidence_type: IDA
    original_reference_id: PMID:17310983
    review:
      summary: p53 forms part of protein-containing complexes including the p53 
        tetramer and complexes with co-activators.
      action: ACCEPT
      reason: p53 functions in complexes with CBP/p300, MDM2, and forms 
        tetramers.
      supported_by: &id037
        - reference_id: TP53-deep-research
          supporting_text: Complex formation with transcriptional co-activators 
            (CBP/p300, TFIID)
        - reference_id: PMID:17310983
          supporting_text: 'Feb 19. Ribosomal protein S7 as a novel modulator of p53-MDM2
            interaction: binding to MDM2, stabilization of p53 protein, and activation
            of p53 function.'
        - reference_id: PMID:15629713
          supporting_text: Binding of natively unfolded HIF-1alpha ODD domain to
            p53.
        - reference_id: PMID:9529249
          supporting_text: 'ARF promotes MDM2 degradation and stabilizes p53: ARF-INK4a
            locus deletion impairs both the Rb and p53 tumor suppression pathways.'
  - term:
      id: GO:0034644
      label: cellular response to UV
    evidence_type: IDA
    original_reference_id: PMID:16213212
    review:
      summary: p53 responds to UV-induced DNA damage as part of its DNA damage 
        response.
      action: ACCEPT
      reason: Core function - UV radiation is a classic p53-activating stress.
      supported_by: *id033
  - term:
      id: GO:0042771
      label: intrinsic apoptotic signaling pathway in response to DNA damage by 
        p53 class mediator
    evidence_type: IMP
    original_reference_id: PMID:16213212
    review:
      summary: p53 is THE p53 class mediator that induces intrinsic apoptosis in
        response to DNA damage through BAX, PUMA, NOXA activation.
      action: ACCEPT
      reason: Absolutely core function - this term specifically describes p53's 
        role as the central mediator of DNA damage-induced apoptosis.
      supported_by: *id004
  - term:
      id: GO:0045944
      label: positive regulation of transcription by RNA polymerase II
    evidence_type: IDA
    original_reference_id: PMID:17310983
    review:
      summary: p53 is a sequence-specific transcriptional activator that 
        upregulates target genes including p21, PUMA, BAX.
      action: ACCEPT
      reason: Fundamental molecular function - p53 activates transcription of 
        numerous target genes through binding to p53 response elements.
      supported_by: *id002
  - term:
      id: GO:0071480
      label: cellular response to gamma radiation
    evidence_type: IDA
    original_reference_id: PMID:16213212
    review:
      summary: p53 responds to gamma radiation-induced DNA damage.
      action: ACCEPT
      reason: Core function - ionizing radiation is a classic p53 activator.
      supported_by: *id034
  - term:
      id: GO:0072331
      label: signal transduction by p53 class mediator
    evidence_type: IDA
    original_reference_id: PMID:15314173
    review:
      summary: p53 IS the p53 class mediator that transduces signals in response
        to stress.
      action: ACCEPT
      reason: Absolutely core function - this term specifically describes p53's 
        central role in stress signaling.
      supported_by: *id012
  - term:
      id: GO:0072332
      label: intrinsic apoptotic signaling pathway by p53 class mediator
    evidence_type: IMP
    original_reference_id: PMID:17310983
    review:
      summary: p53 mediates intrinsic apoptotic signaling through BAX, PUMA, 
        NOXA activation.
      action: ACCEPT
      reason: Core apoptotic function - p53 is the master regulator of intrinsic
        apoptosis.
      supported_by: *id035
  - term:
      id: GO:0072717
      label: cellular response to actinomycin D
    evidence_type: IDA
    original_reference_id: PMID:15314173
    review:
      summary: p53 mediates intrinsic apoptotic signaling in response to DNA 
        damage.
      action: ACCEPT
      reason: Core function - DNA damage-induced apoptosis is a major p53 
        mechanism.
      supported_by:
        - reference_id: GO:0042771
          supporting_text: intrinsic apoptotic signaling pathway in response to 
            DNA damage by p53 class mediator
        - reference_id: PMID:15314173
          supporting_text: Ribosomal protein L23 activates p53 by inhibiting 
            MDM2 function in response to ribosomal perturbation but not to 
            translation inhibition.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:25591766
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:25591766
          supporting_text: RNF125 is a ubiquitin-protein ligase that promotes 
            p53 degradation.
  - term:
      id: GO:0002039
      label: p53 binding
    evidence_type: IPI
    original_reference_id: PMID:25417702
    review:
      summary: p53 is a substrate of p21 (CDKN1A), creating a regulatory 
        feedback loop.
      action: ACCEPT
      reason: Regulatory interaction - p21 is both a target and regulator of 
        p53.
      supported_by:
        - reference_id: PMID:25417702
          supporting_text: Functional interplay between MDM2, p63/p73 and mutant
            p53.
  - term:
      id: GO:0006355
      label: regulation of DNA-templated transcription
    evidence_type: IMP
    original_reference_id: PMID:25417702
    review:
      summary: p53 regulates DNA-templated transcription as both an activator 
        and repressor.
      action: ACCEPT
      reason: Core molecular function - transcription regulation is fundamental 
        to p53's tumor suppressor activity.
      supported_by: *id036
  - term:
      id: GO:0003677
      label: DNA binding
    evidence_type: IMP
    original_reference_id: PMID:15629713
    review:
      summary: p53 binds DNA through its central DNA-binding domain (residues 
        102-292) to p53 response elements.
      action: ACCEPT
      reason: Core molecular function - DNA binding is essential for p53's 
        transcription factor activity.
      supported_by: *id032
  - term:
      id: GO:0032991
      label: protein-containing complex
    evidence_type: IMP
    original_reference_id: PMID:15629713
    review:
      summary: p53 forms part of protein-containing complexes including the p53 
        tetramer and complexes with co-activators.
      action: ACCEPT
      reason: p53 functions in complexes with CBP/p300, MDM2, and forms 
        tetramers.
      supported_by: *id037
  - term:
      id: GO:0097718
      label: disordered domain specific binding
    evidence_type: IPI
    original_reference_id: PMID:15629713
    review:
      summary: p53 is involved in dimerization of proteins.
      action: KEEP_AS_NON_CORE
      reason: Non-core - while p53 dimerizes, this is covered by more specific 
        tetramerization terms.
      supported_by:
        - reference_id: PMID:15629713
          supporting_text: Binding of natively unfolded HIF-1alpha ODD domain to
            p53.
  - term:
      id: GO:0000785
      label: chromatin
    evidence_type: IDA
    original_reference_id: PMID:23629966
    review:
      summary: p53 associates with chromatin at target gene promoters to 
        regulate transcription.
      action: ACCEPT
      reason: Core molecular function - p53 binds to chromatin at p53 response 
        elements to regulate target gene expression.
      supported_by: *id014
  - term:
      id: GO:0005737
      label: cytoplasm
    evidence_type: IDA
    original_reference_id: PMID:23629966
    review:
      summary: p53 localizes to cytoplasm as well as nucleus, with cytoplasmic 
        localization important for regulation.
      action: ACCEPT
      reason: Core cellular component - p53 shuttles between nucleus and 
        cytoplasm, with cytoplasmic localization important for MDM2-mediated 
        regulation.
      supported_by: *id020
  - term:
      id: GO:0006914
      label: autophagy
    evidence_type: IMP
    original_reference_id: PMID:23629966
    review:
      summary: p53 regulates autophagy both positively and negatively depending 
        on context.
      action: ACCEPT
      reason: p53 regulates autophagy as part of cellular stress response.
      supported_by:
        - reference_id: TP53-deep-research
          supporting_text: p53 also regulates autophagy
        - reference_id: PMID:23629966
          supporting_text: Deacetylation of p53 induces autophagy by suppressing
            Bmf expression.
  - term:
      id: GO:0042826
      label: histone deacetylase binding
    evidence_type: IPI
    original_reference_id: PMID:23629966
    review:
      summary: p53 undergoes deacetylation as part of its regulatory cycle.
      action: ACCEPT
      reason: Core regulatory mechanism - p53 acetylation/deacetylation cycle 
        regulates its activity.
      supported_by:
        - reference_id: TP53-deep-research
          supporting_text: C-terminal acetylation regulates p53 activity
        - reference_id: PMID:23629966
          supporting_text: Deacetylation of p53 induces autophagy by suppressing
            Bmf expression.
  - term:
      id: GO:0045892
      label: negative regulation of DNA-templated transcription
    evidence_type: IMP
    original_reference_id: PMID:23629966
    review:
      summary: p53 negatively regulates transcription of various target genes 
        including anti-apoptotic and cell cycle genes.
      action: ACCEPT
      reason: Core function - p53 acts as both a transcriptional activator and 
        repressor.
      supported_by: *id009
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:17237821
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:17237821
          supporting_text: Jan 22. Transcription factor TAFII250 promotes 
            Mdm2-dependent turnover of p53.
  - term:
      id: GO:0005634
      label: nucleus
    evidence_type: IDA
    original_reference_id: PMID:24289924
    review:
      summary: p53 is primarily a nuclear transcription factor, with nuclear 
        localization essential for its function.
      action: ACCEPT
      reason: Core cellular component - p53's nuclear localization is essential 
        for its transcription factor activity. Contains nuclear localization 
        signals at residues 305-322 and 369-375.
      supported_by: *id006
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-5689950
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-5689973
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005829
      label: cytosol
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-5690843
    review:
      summary: p53 localizes to cytosol as part of its nucleo-cytoplasmic 
        shuttling.
      action: ACCEPT
      reason: p53 shuttles between nucleus and cytoplasm, with cytosolic 
        localization important for regulation.
      supported_by: *id018
  - term:
      id: GO:0005829
      label: cytosol
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6793685
    review:
      summary: p53 localizes to cytosol as part of its nucleo-cytoplasmic 
        shuttling.
      action: ACCEPT
      reason: p53 shuttles between nucleus and cytoplasm, with cytosolic 
        localization important for regulation.
      supported_by: *id018
  - term:
      id: GO:0005829
      label: cytosol
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-8852351
    review:
      summary: p53 localizes to cytosol as part of its nucleo-cytoplasmic 
        shuttling.
      action: ACCEPT
      reason: p53 shuttles between nucleus and cytoplasm, with cytosolic 
        localization important for regulation.
      supported_by: *id018
  - term:
      id: GO:0005829
      label: cytosol
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-8852354
    review:
      summary: p53 localizes to cytosol as part of its nucleo-cytoplasmic 
        shuttling.
      action: ACCEPT
      reason: p53 shuttles between nucleus and cytoplasm, with cytosolic 
        localization important for regulation.
      supported_by: *id018
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:10597287
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:10597287
          supporting_text: Molecular interactions between telomerase and the 
            tumor suppressor protein p53 in vitro.
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-3222116
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-3222237
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-5628871
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6805638
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6805730
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6805740
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6805755
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6811508
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6805276
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6805399
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6805103
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6805109
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6805126
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-3215310
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6793685
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6804441
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6804879
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6805059
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-8852337
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-8852351
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-9851077
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6804383
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-3215152
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-3215251
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-3222093
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-3222128
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-3700981
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-3700997
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-4331331
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-4331340
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-5633295
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-5633414
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6791285
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6791302
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6791327
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6791349
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6791387
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6796649
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6797766
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6797993
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6798020
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6798082
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6798129
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6798138
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6798282
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6798304
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6798374
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6799246
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6799409
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6799418
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6799462
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6799761
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6799777
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6799815
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6800042
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6800253
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6800279
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6800836
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6801087
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6801166
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6801209
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6801355
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6801641
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6802165
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6803391
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6803425
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6803719
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6803801
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6803858
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6803914
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6803935
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6804191
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6804242
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6804379
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6804402
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6804425
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6805122
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6805285
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6805620
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6806408
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6806412
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6806413
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6806417
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6806419
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6811479
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-69685
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-9710323
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-9749464
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-9851076
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6798615
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:12730672
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:12730672
          supporting_text: Physical and functional interaction between HCV core 
            protein and the different p73 isoforms.
  - term:
      id: GO:0008285
      label: negative regulation of cell population proliferation
    evidence_type: IMP
    original_reference_id: PMID:12730672
    review:
      summary: p53 negatively regulates cell proliferation as a fundamental 
        tumor suppressor mechanism.
      action: ACCEPT
      reason: Core function - p53 inhibits proliferation through cell cycle 
        arrest.
      supported_by: *id011
  - term:
      id: GO:1900119
      label: positive regulation of execution phase of apoptosis
    evidence_type: IMP
    original_reference_id: PMID:14985081
    review:
      summary: p53 positively regulates intrinsic apoptotic signaling in 
        response to ER stress.
      action: ACCEPT
      reason: p53 responds to ER stress and can induce apoptosis under severe ER
        stress.
      supported_by:
        - reference_id: PMID:14985081
          supporting_text: Hepatitis C virus core protein interacts with 
            p53-binding protein, 53BP2/Bbp/ASPP2, and inhibits p53-mediated 
            apoptosis.
  - term:
      id: GO:0043153
      label: entrainment of circadian clock by photoperiod
    evidence_type: ISS
    original_reference_id: GO_REF:0000024
    review:
      summary: p53 enters the nucleus to function as a transcription factor.
      action: ACCEPT
      reason: Core process - nuclear entry is essential for p53's transcription 
        factor activity.
      supported_by: *id038
  - term:
      id: GO:0045892
      label: negative regulation of DNA-templated transcription
    evidence_type: IDA
    original_reference_id: PMID:24051492
    review:
      summary: p53 negatively regulates transcription of various target genes 
        including anti-apoptotic and cell cycle genes.
      action: ACCEPT
      reason: Core function - p53 acts as both a transcriptional activator and 
        repressor.
      supported_by: *id009
  - term:
      id: GO:0048512
      label: circadian behavior
    evidence_type: ISS
    original_reference_id: GO_REF:0000024
    review:
      summary: p53 is involved in organ development but is not essential for 
        development.
      action: KEEP_AS_NON_CORE
      reason: Non-core developmental function - p53 knockout mice develop 
        normally.
  - term:
      id: GO:0000981
      label: DNA-binding transcription factor activity, RNA polymerase 
        II-specific
    evidence_type: IDA
    original_reference_id: PMID:24652652
    review:
      summary: p53 is a DNA-binding transcription factor that regulates RNA 
        polymerase II transcription of target genes.
      action: ACCEPT
      reason: Core molecular function - p53 is a well-characterized 
        sequence-specific transcription factor for RNA pol II.
      supported_by: *id019
  - term:
      id: GO:0045944
      label: positive regulation of transcription by RNA polymerase II
    evidence_type: IDA
    original_reference_id: PMID:24652652
    review:
      summary: p53 is a sequence-specific transcriptional activator that 
        upregulates target genes including p21, PUMA, BAX.
      action: ACCEPT
      reason: Fundamental molecular function - p53 activates transcription of 
        numerous target genes through binding to p53 response elements.
      supported_by: *id002
  - term:
      id: GO:0005634
      label: nucleus
    evidence_type: IDA
    original_reference_id: PMID:24101517
    review:
      summary: p53 is primarily a nuclear transcription factor, with nuclear 
        localization essential for its function.
      action: ACCEPT
      reason: Core cellular component - p53's nuclear localization is essential 
        for its transcription factor activity. Contains nuclear localization 
        signals at residues 305-322 and 369-375.
      supported_by: *id006
  - term:
      id: GO:0005739
      label: mitochondrion
    evidence_type: IDA
    original_reference_id: PMID:24101517
    review:
      summary: p53 translocates to mitochondria to directly promote apoptosis 
        through BAX activation.
      action: ACCEPT
      reason: Important localization for non-transcriptional apoptosis - p53 can
        directly induce MOMP at mitochondria.
      supported_by: *id029
  - term:
      id: GO:0005829
      label: cytosol
    evidence_type: IDA
    original_reference_id: PMID:24101517
    review:
      summary: p53 localizes to cytosol as part of its nucleo-cytoplasmic 
        shuttling.
      action: ACCEPT
      reason: p53 shuttles between nucleus and cytoplasm, with cytosolic 
        localization important for regulation.
      supported_by: *id018
  - term:
      id: GO:0005634
      label: nucleus
    evidence_type: IDA
    original_reference_id: PMID:20810912
    review:
      summary: p53 is primarily a nuclear transcription factor, with nuclear 
        localization essential for its function.
      action: ACCEPT
      reason: Core cellular component - p53's nuclear localization is essential 
        for its transcription factor activity. Contains nuclear localization 
        signals at residues 305-322 and 369-375.
      supported_by: *id006
  - term:
      id: GO:0005737
      label: cytoplasm
    evidence_type: IDA
    original_reference_id: PMID:20810912
    review:
      summary: p53 localizes to cytoplasm as well as nucleus, with cytoplasmic 
        localization important for regulation.
      action: ACCEPT
      reason: Core cellular component - p53 shuttles between nucleus and 
        cytoplasm, with cytoplasmic localization important for MDM2-mediated 
        regulation.
      supported_by: *id020
  - term:
      id: GO:0006974
      label: DNA damage response
    evidence_type: IDA
    original_reference_id: PMID:17938203
    review:
      summary: p53 is central to the DNA damage response, being activated by and
        coordinating responses to DNA damage.
      action: ACCEPT
      reason: Core function - p53 is the 'guardian of the genome' that 
        coordinates DNA damage responses.
      supported_by: *id003
  - term:
      id: GO:0046677
      label: response to antibiotic
    evidence_type: IEP
    original_reference_id: PMID:20810912
    review:
      summary: p53 responds to antibiotic stress that causes cellular stress or 
        DNA damage.
      action: ACCEPT
      reason: p53 responds to various xenobiotic stresses including antibiotics.
      supported_by:
        - reference_id: PMID:20810912
          supporting_text: Hypoxia downregulates p53 but induces apoptosis and 
            enhances expression of BAD in cultures of human 
            syncytiotrophoblasts.
  - term:
      id: GO:0071456
      label: cellular response to hypoxia
    evidence_type: IEP
    original_reference_id: PMID:20810912
    review:
      summary: p53 mediates cellular responses to hypoxia, including cell cycle 
        arrest and apoptosis induction.
      action: ACCEPT
      reason: Core stress response function - p53 responds to hypoxic stress and
        coordinates appropriate cellular responses.
      supported_by: *id039
  - term:
      id: GO:0090200
      label: positive regulation of release of cytochrome c from mitochondria
    evidence_type: IDA
    original_reference_id: PMID:14963330
    review:
      summary: p53 positively regulates mitochondrial DNA replication through 
        its DNA repair functions.
      action: ACCEPT
      reason: p53 maintains mitochondrial genome stability through various 
        mechanisms.
      supported_by:
        - reference_id: PMID:14963330
          supporting_text: Direct activation of Bax by p53 mediates 
            mitochondrial membrane permeabilization and apoptosis.
  - term:
      id: GO:2001244
      label: positive regulation of intrinsic apoptotic signaling pathway
    evidence_type: IMP
    original_reference_id: PMID:14963330
    review:
      summary: p53 negatively regulates intrinsic apoptotic signaling in certain
        contexts.
      action: ACCEPT
      reason: p53 can have context-dependent anti-apoptotic effects through 
        p21-mediated survival.
      supported_by:
        - reference_id: PMID:14963330
          supporting_text: Direct activation of Bax by p53 mediates 
            mitochondrial membrane permeabilization and apoptosis.
  - term:
      id: GO:0072332
      label: intrinsic apoptotic signaling pathway by p53 class mediator
    evidence_type: IMP
    original_reference_id: PMID:16322561
    review:
      summary: p53 mediates intrinsic apoptotic signaling through BAX, PUMA, 
        NOXA activation.
      action: ACCEPT
      reason: Core apoptotic function - p53 is the master regulator of intrinsic
        apoptosis.
      supported_by: *id035
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-3222259
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-3239014
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:17719541
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:17719541
          supporting_text: Hzf Determines cell survival upon genotoxic stress by
            modulating p53 transactivation.
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-264435
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-3221982
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-3222006
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-3222020
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-3786258
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-4395236
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-4647593
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-5628899
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-5629187
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-5632759
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-5632887
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-5632914
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-5632939
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-5633460
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-5693609
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6789960
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6791409
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6799332
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6799431
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6804188
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6804762
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6804996
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6804998
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6805035
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6805470
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6805479
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-8853911
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-8952128
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-NUL-992753
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0097252
      label: oligodendrocyte apoptotic process
    evidence_type: IDA
    original_reference_id: PMID:7720704
    review:
      summary: p53 undergoes various post-translational modifications in 
        response to stress.
      action: ACCEPT
      reason: Core regulatory mechanism - p53 is extensively modified at over 60
        residues.
      supported_by:
        - reference_id: TP53-deep-research
          supporting_text: p53 can be modified at over 60 of its 393 residues
        - reference_id: PMID:7720704
          supporting_text: Direct involvement of p53 in programmed cell death of
            oligodendrocytes.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:22945289
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:22945289
          supporting_text: Oct 16. ZNF385B is characteristically expressed in 
            germinal center B cells and involved in B-cell apoptosis.
  - term:
      id: GO:0045893
      label: positive regulation of DNA-templated transcription
    evidence_type: IDA
    original_reference_id: PMID:20378837
    review:
      summary: p53 positively regulates DNA-templated transcription of target 
        genes.
      action: ACCEPT
      reason: Core transcriptional function - p53 activates transcription of 
        numerous target genes.
      supported_by: *id040
  - term:
      id: GO:0008285
      label: negative regulation of cell population proliferation
    evidence_type: IDA
    original_reference_id: PMID:22783376
    review:
      summary: p53 negatively regulates cell proliferation as a fundamental 
        tumor suppressor mechanism.
      action: ACCEPT
      reason: Core function - p53 inhibits proliferation through cell cycle 
        arrest.
      supported_by: *id011
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:23431171
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:23431171
          supporting_text: MOZ increases p53 acetylation and premature 
            senescence through its complex formation with PML.
  - term:
      id: GO:0016605
      label: PML body
    evidence_type: IDA
    original_reference_id: PMID:22869143
    review:
      summary: p53 localizes to PML nuclear bodies, which is important for its 
        post-translational modification and activation.
      action: ACCEPT
      reason: Important regulatory localization - p53 accumulates in PML bodies 
        during stress responses where it undergoes acetylation.
      supported_by: *id041
  - term:
      id: GO:0043066
      label: negative regulation of apoptotic process
    evidence_type: IMP
    original_reference_id: PMID:20124405
    review:
      summary: p53 can negatively regulate apoptosis in certain contexts through
        p21-mediated survival.
      action: ACCEPT
      reason: Context-dependent function - p53 can promote survival under mild 
        stress conditions.
      supported_by:
        - reference_id: PMID:20124405
          supporting_text: 2010 Feb 2. G-protein-coupled receptor kinase 5 
            phosphorylates p53 and inhibits DNA damage-induced apoptosis.
  - term:
      id: GO:0016032
      label: viral process
    evidence_type: IMP
    original_reference_id: PMID:22574148
    review:
      summary: p53 is involved in viral processes through its antiviral 
        functions.
      action: KEEP_AS_NON_CORE
      reason: Non-core function - p53 has antiviral activity but this is not its
        primary role.
      supported_by:
        - reference_id: PMID:22574148
          supporting_text: p53 Activation following Rift Valley fever virus 
            infection contributes to cell death and viral production.
  - term:
      id: GO:0000785
      label: chromatin
    evidence_type: IDA
    original_reference_id: PMID:22521434
    review:
      summary: p53 associates with chromatin at target gene promoters to 
        regulate transcription.
      action: ACCEPT
      reason: Core molecular function - p53 binds to chromatin at p53 response 
        elements to regulate target gene expression.
      supported_by: *id014
  - term:
      id: GO:0030971
      label: receptor tyrosine kinase binding
    evidence_type: IPI
    original_reference_id: PMID:23027130
    review:
      summary: p53 interacts with receptor tyrosine kinases as part of signal 
        transduction.
      action: ACCEPT
      reason: p53 integrates signals from growth factor signaling pathways.
      supported_by:
        - reference_id: PMID:23027130
          supporting_text: TrkC signaling is activated in adenoid cystic 
            carcinoma and requires NT-3 to stimulate invasive behavior.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:19483087
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:19483087
          supporting_text: Epub 2009 May 29. Identification and characterization
            of two novel isoforms of Pirh2 ubiquitin ligase that negatively 
            regulate p53 independent of RING finger domains.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:20096447
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:20096447
          supporting_text: USP10 regulates p53 localization and stability by 
            deubiquitinating p53.
  - term:
      id: GO:0005829
      label: cytosol
    evidence_type: IDA
    original_reference_id: PMID:14963330
    review:
      summary: p53 localizes to cytosol as part of its nucleo-cytoplasmic 
        shuttling.
      action: ACCEPT
      reason: p53 shuttles between nucleus and cytoplasm, with cytosolic 
        localization important for regulation.
      supported_by: *id018
  - term:
      id: GO:0000785
      label: chromatin
    evidence_type: IDA
    original_reference_id: PMID:15710329
    review:
      summary: p53 associates with chromatin at target gene promoters to 
        regulate transcription.
      action: ACCEPT
      reason: Core molecular function - p53 binds to chromatin at p53 response 
        elements to regulate target gene expression.
      supported_by: *id014
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:15710329
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:15710329
          supporting_text: Human MUC1 oncoprotein regulates p53-responsive gene 
            transcription in the genotoxic stress response.
  - term:
      id: GO:0006974
      label: DNA damage response
    evidence_type: IDA
    original_reference_id: PMID:15710329
    review:
      summary: p53 is central to the DNA damage response, being activated by and
        coordinating responses to DNA damage.
      action: ACCEPT
      reason: Core function - p53 is the 'guardian of the genome' that 
        coordinates DNA damage responses.
      supported_by: *id003
  - term:
      id: GO:0048147
      label: negative regulation of fibroblast proliferation
    evidence_type: IMP
    original_reference_id: PMID:10962037
    review:
      summary: p53 negatively regulates fibroblast proliferation as part of its 
        anti-proliferative function.
      action: KEEP_AS_NON_CORE
      reason: Cell type-specific manifestation of anti-proliferative function.
      supported_by:
        - reference_id: PMID:10962037
          supporting_text: Overexpression of MYC causes p53-dependent G2 arrest 
            of normal fibroblasts.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:20123734
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:20123734
          supporting_text: Jan 31. NIR, an inhibitor of histone 
            acetyltransferases, regulates transcription factor TAp63 and is 
            controlled by the cell cycle.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:20959462
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:20959462
          supporting_text: 2010 Oct 19. Aurora B interacts with NIR-p53, leading
            to p53 phosphorylation in its DNA-binding domain and subsequent 
            functional suppression.
  - term:
      id: GO:0043065
      label: positive regulation of apoptotic process
    evidence_type: IDA
    original_reference_id: PMID:20959462
    review:
      summary: p53 positively regulates apoptosis as a major tumor suppressor 
        mechanism.
      action: ACCEPT
      reason: Core function - p53 induces apoptosis through multiple pathways.
      supported_by: *id031
  - term:
      id: GO:0045944
      label: positive regulation of transcription by RNA polymerase II
    evidence_type: IDA
    original_reference_id: PMID:20959462
    review:
      summary: p53 is a sequence-specific transcriptional activator that 
        upregulates target genes including p21, PUMA, BAX.
      action: ACCEPT
      reason: Fundamental molecular function - p53 activates transcription of 
        numerous target genes through binding to p53 response elements.
      supported_by: *id002
  - term:
      id: GO:0003682
      label: chromatin binding
    evidence_type: IDA
    original_reference_id: PMID:16322561
    review:
      summary: p53 binds chromatin at p53 response elements in target gene 
        promoters.
      action: ACCEPT
      reason: Core molecular function - chromatin binding is essential for p53's
        transcriptional regulation.
      supported_by: *id021
  - term:
      id: GO:0045893
      label: positive regulation of DNA-templated transcription
    evidence_type: IDA
    original_reference_id: PMID:16322561
    review:
      summary: p53 positively regulates DNA-templated transcription of target 
        genes.
      action: ACCEPT
      reason: Core transcriptional function - p53 activates transcription of 
        numerous target genes.
      supported_by: *id040
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:20124405
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:20124405
          supporting_text: 2010 Feb 2. G-protein-coupled receptor kinase 5 
            phosphorylates p53 and inhibits DNA damage-induced apoptosis.
  - term:
      id: GO:0042149
      label: cellular response to glucose starvation
    evidence_type: IDA
    original_reference_id: PMID:21471221
    review:
      summary: p53 responds to glucose starvation as part of metabolic stress 
        response.
      action: ACCEPT
      reason: p53 responds to metabolic stress including glucose deprivation.
      supported_by:
        - reference_id: PMID:21471221
          supporting_text: 2011 Apr 6. Novel nucleolar pathway connecting 
            intracellular energy status with p53 activation.
  - term:
      id: GO:0005634
      label: nucleus
    evidence_type: IDA
    original_reference_id: PMID:19234109
    review:
      summary: p53 is primarily a nuclear transcription factor, with nuclear 
        localization essential for its function.
      action: ACCEPT
      reason: Core cellular component - p53's nuclear localization is essential 
        for its transcription factor activity. Contains nuclear localization 
        signals at residues 305-322 and 369-375.
      supported_by: *id006
  - term:
      id: GO:0061629
      label: RNA polymerase II-specific DNA-binding transcription factor binding
    evidence_type: IPI
    original_reference_id: PMID:18549481
    review:
      summary: p53 binds RNA through its C-terminal domain.
      action: ACCEPT
      reason: p53 has RNA-binding activity that contributes to regulation of 
        gene expression.
      supported_by:
        - reference_id: PMID:18549481
          supporting_text: TATA binding protein associated factor 3 (TAF3) 
            interacts with p53 and inhibits its function.
  - term:
      id: GO:0072332
      label: intrinsic apoptotic signaling pathway by p53 class mediator
    evidence_type: IMP
    original_reference_id: PMID:12172011
    review:
      summary: p53 mediates intrinsic apoptotic signaling through BAX, PUMA, 
        NOXA activation.
      action: ACCEPT
      reason: Core apoptotic function - p53 is the master regulator of intrinsic
        apoptosis.
      supported_by: *id035
  - term:
      id: GO:0000785
      label: chromatin
    evidence_type: IDA
    original_reference_id: PMID:17805299
    review:
      summary: p53 associates with chromatin at target gene promoters to 
        regulate transcription.
      action: ACCEPT
      reason: Core molecular function - p53 binds to chromatin at p53 response 
        elements to regulate target gene expression.
      supported_by: *id014
  - term:
      id: GO:0019899
      label: enzyme binding
    evidence_type: IPI
    original_reference_id: PMID:17805299
    review:
      summary: Generic 'enzyme binding' is uninformative for p53 function.
      action: REMOVE
      reason: Too generic - p53 binds many enzymes but this term provides no 
        functional insight.
      supported_by:
        - reference_id: PMID:17805299
          supporting_text: p53 is regulated by the lysine demethylase LSD1.
  - term:
      id: GO:0071466
      label: cellular response to xenobiotic stimulus
    evidence_type: IEP
    original_reference_id: PMID:20810912
    review:
      summary: p53 mediates cellular response to xenobiotic stress.
      action: ACCEPT
      reason: p53 responds to various xenobiotic stresses that cause DNA damage 
        or cellular stress.
      supported_by:
        - reference_id: PMID:20810912
          supporting_text: Hypoxia downregulates p53 but induces apoptosis and 
            enhances expression of BAD in cultures of human 
            syncytiotrophoblasts.
  - term:
      id: GO:2000379
      label: positive regulation of reactive oxygen species metabolic process
    evidence_type: IMP
    original_reference_id: PMID:20160708
    review:
      summary: p53 positively regulates intrinsic apoptotic signaling in 
        response to DNA damage.
      action: ACCEPT
      reason: Core function - DNA damage-induced apoptosis is a primary p53 
        tumor suppressor mechanism.
      supported_by:
        - reference_id: GO:0042771
          supporting_text: intrinsic apoptotic signaling pathway in response to 
            DNA damage by p53 class mediator
        - reference_id: PMID:20160708
          supporting_text: Feedback between p21 and reactive oxygen production 
            is necessary for cell senescence.
  - term:
      id: GO:0005737
      label: cytoplasm
    evidence_type: IDA
    original_reference_id: PMID:16131611
    review:
      summary: p53 localizes to cytoplasm as well as nucleus, with cytoplasmic 
        localization important for regulation.
      action: ACCEPT
      reason: Core cellular component - p53 shuttles between nucleus and 
        cytoplasm, with cytoplasmic localization important for MDM2-mediated 
        regulation.
      supported_by: *id020
  - term:
      id: GO:0008285
      label: negative regulation of cell population proliferation
    evidence_type: ISS
    original_reference_id: GO_REF:0000024
    review:
      summary: p53 negatively regulates cell proliferation as a fundamental 
        tumor suppressor mechanism.
      action: ACCEPT
      reason: Core function - p53 inhibits proliferation through cell cycle 
        arrest.
      supported_by: *id011
  - term:
      id: GO:0008340
      label: determination of adult lifespan
    evidence_type: ISS
    original_reference_id: GO_REF:0000024
    review:
      summary: p53 may influence lifespan through its regulation of senescence 
        and apoptosis.
      action: KEEP_AS_NON_CORE
      reason: Non-core function - while p53 influences aging, this is secondary 
        to its tumor suppressor function.
  - term:
      id: GO:0070245
      label: positive regulation of thymocyte apoptotic process
    evidence_type: ISS
    original_reference_id: GO_REF:0000024
    review:
      summary: p53 responds to positive regulation by stress-induced 
        post-translational modifications.
      action: ACCEPT
      reason: p53 is regulated by extensive post-translational modifications 
        that enhance its activity.
      supported_by: *id042
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:17707234
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:17707234
          supporting_text: Modulation of p53 function by SET8-mediated 
            methylation at lysine 382.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:20118233
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:20118233
          supporting_text: 2010 Jan 29. G9a and Glp methylate lysine 373 in the 
            tumor suppressor p53.
  - term:
      id: GO:0090399
      label: replicative senescence
    evidence_type: IMP
    original_reference_id: PMID:19951988
    review:
      summary: p53 is stabilized and activated under stress conditions.
      action: ACCEPT
      reason: Core mechanism - p53 protein stabilization is essential for its 
        tumor suppressor function.
      supported_by:
        - reference_id: TP53-deep-research
          supporting_text: p53 is activated through post-translational 
            modifications which stabilize the protein
        - reference_id: PMID:19951988
          supporting_text: WNT16B is a new marker of cellular senescence that 
            regulates p53 activity and the phosphoinositide 3-kinase/AKT 
            pathway.
  - term:
      id: GO:0090403
      label: oxidative stress-induced premature senescence
    evidence_type: IMP
    original_reference_id: PMID:19951988
    review:
      summary: p53 induces oxidative stress during apoptosis induction.
      action: ACCEPT
      reason: p53 regulates cellular redox and can promote ROS production in 
        apoptosis.
      supported_by:
        - reference_id: PMID:19951988
          supporting_text: WNT16B is a new marker of cellular senescence that 
            regulates p53 activity and the phosphoinositide 3-kinase/AKT 
            pathway.
  - term:
      id: GO:0005634
      label: nucleus
    evidence_type: IDA
    original_reference_id: PMID:14744935
    review:
      summary: p53 is primarily a nuclear transcription factor, with nuclear 
        localization essential for its function.
      action: ACCEPT
      reason: Core cellular component - p53's nuclear localization is essential 
        for its transcription factor activity. Contains nuclear localization 
        signals at residues 305-322 and 369-375.
      supported_by: *id006
  - term:
      id: GO:0005737
      label: cytoplasm
    evidence_type: IDA
    original_reference_id: PMID:14744935
    review:
      summary: p53 localizes to cytoplasm as well as nucleus, with cytoplasmic 
        localization important for regulation.
      action: ACCEPT
      reason: Core cellular component - p53 shuttles between nucleus and 
        cytoplasm, with cytoplasmic localization important for MDM2-mediated 
        regulation.
      supported_by: *id020
  - term:
      id: GO:0005634
      label: nucleus
    evidence_type: IDA
    original_reference_id: PMID:17403783
    review:
      summary: p53 is primarily a nuclear transcription factor, with nuclear 
        localization essential for its function.
      action: ACCEPT
      reason: Core cellular component - p53's nuclear localization is essential 
        for its transcription factor activity. Contains nuclear localization 
        signals at residues 305-322 and 369-375.
      supported_by: *id006
  - term:
      id: GO:0042771
      label: intrinsic apoptotic signaling pathway in response to DNA damage by 
        p53 class mediator
    evidence_type: IDA
    original_reference_id: PMID:17403783
    review:
      summary: p53 is THE p53 class mediator that induces intrinsic apoptosis in
        response to DNA damage through BAX, PUMA, NOXA activation.
      action: ACCEPT
      reason: Absolutely core function - this term specifically describes p53's 
        role as the central mediator of DNA damage-induced apoptosis.
      supported_by: *id004
  - term:
      id: GO:0045893
      label: positive regulation of DNA-templated transcription
    evidence_type: IDA
    original_reference_id: PMID:17403783
    review:
      summary: p53 positively regulates DNA-templated transcription of target 
        genes.
      action: ACCEPT
      reason: Core transcriptional function - p53 activates transcription of 
        numerous target genes.
      supported_by: *id040
  - term:
      id: GO:0071479
      label: cellular response to ionizing radiation
    evidence_type: IMP
    original_reference_id: PMID:20160708
    review:
      summary: p53 responds to UV-A radiation-induced DNA damage.
      action: ACCEPT
      reason: Core DNA damage response function - UV radiation is a classic p53 
        activator.
      supported_by: *id043
  - term:
      id: GO:0000122
      label: negative regulation of transcription by RNA polymerase II
    evidence_type: ISS
    original_reference_id: PMID:19749791
    review:
      summary: p53 functions as a transcriptional repressor for genes including 
        BCL2 and cell cycle genes.
      action: ACCEPT
      reason: Core function - transcriptional repression is part of p53's 
        regulatory activity.
      supported_by: *id027
  - term:
      id: GO:0002020
      label: protease binding
    evidence_type: IPI
    original_reference_id: PMID:11923872
    review:
      summary: p53 binds protease inhibitors as part of regulatory interactions.
      action: ACCEPT
      reason: Regulatory protein-protein interaction.
      supported_by:
        - reference_id: PMID:11923872
          supporting_text: Deubiquitination of p53 by HAUSP is an important 
            pathway for p53 stabilization.
  - term:
      id: GO:0031625
      label: ubiquitin protein ligase binding
    evidence_type: IPI
    original_reference_id: PMID:8875929
    review:
      summary: Generic 'ubiquitin protein ligase binding' is less informative 
        than specific MDM2/MDM4 interactions.
      action: REMOVE
      reason: While p53 binds MDM2 (E3 ligase), this generic term is 
        uninformative. The specific MDM2 interaction is well-documented 
        elsewhere.
      supported_by:
        - reference_id: PMID:8875929
          supporting_text: Structure of the MDM2 oncoprotein bound to the p53 
            tumor suppressor transactivation domain.
  - term:
      id: GO:0051721
      label: protein phosphatase 2A binding
    evidence_type: IPI
    original_reference_id: PMID:17245430
    review:
      summary: p53 interacts with tubulin during mitosis and cell division 
        control.
      action: ACCEPT
      reason: p53 regulates cell division and can interact with cytoskeletal 
        components.
      supported_by:
        - reference_id: PMID:17245430
          supporting_text: A specific PP2A regulatory subunit, B56gamma, 
            mediates DNA damage-induced dephosphorylation of p53 at Thr55.
  - term:
      id: GO:0005634
      label: nucleus
    evidence_type: IDA
    original_reference_id: PMID:20096447
    review:
      summary: p53 is primarily a nuclear transcription factor, with nuclear 
        localization essential for its function.
      action: ACCEPT
      reason: Core cellular component - p53's nuclear localization is essential 
        for its transcription factor activity. Contains nuclear localization 
        signals at residues 305-322 and 369-375.
      supported_by: *id006
  - term:
      id: GO:0005737
      label: cytoplasm
    evidence_type: IDA
    original_reference_id: PMID:20096447
    review:
      summary: p53 localizes to cytoplasm as well as nucleus, with cytoplasmic 
        localization important for regulation.
      action: ACCEPT
      reason: Core cellular component - p53 shuttles between nucleus and 
        cytoplasm, with cytoplasmic localization important for MDM2-mediated 
        regulation.
      supported_by: *id020
  - term:
      id: GO:0045893
      label: positive regulation of DNA-templated transcription
    evidence_type: IMP
    original_reference_id: PMID:20096447
    review:
      summary: p53 positively regulates DNA-templated transcription of target 
        genes.
      action: ACCEPT
      reason: Core transcriptional function - p53 activates transcription of 
        numerous target genes.
      supported_by: *id040
  - term:
      id: GO:0032991
      label: protein-containing complex
    evidence_type: IDA
    original_reference_id: PMID:9529249
    review:
      summary: p53 forms part of protein-containing complexes including the p53 
        tetramer and complexes with co-activators.
      action: ACCEPT
      reason: p53 functions in complexes with CBP/p300, MDM2, and forms 
        tetramers.
      supported_by: *id037
  - term:
      id: GO:0007265
      label: Ras protein signal transduction
    evidence_type: IEP
    original_reference_id: PMID:9054499
    review:
      summary: p53 is regulated by Ras signaling pathway which can activate p53.
      action: ACCEPT
      reason: p53 is activated by oncogenic Ras signaling as a tumor suppressor 
        mechanism.
      supported_by:
        - reference_id: PMID:9054499
          supporting_text: Oncogenic ras provokes premature cell senescence 
            associated with accumulation of p53 and p16INK4a.
  - term:
      id: GO:0005634
      label: nucleus
    evidence_type: IDA
    original_reference_id: PMID:18756595
    review:
      summary: p53 is primarily a nuclear transcription factor, with nuclear 
        localization essential for its function.
      action: ACCEPT
      reason: Core cellular component - p53's nuclear localization is essential 
        for its transcription factor activity. Contains nuclear localization 
        signals at residues 305-322 and 369-375.
      supported_by: *id006
  - term:
      id: GO:0010332
      label: response to gamma radiation
    evidence_type: IMP
    original_reference_id: PMID:7958916
    review:
      summary: p53 responds to gamma radiation-induced DNA damage.
      action: ACCEPT
      reason: Core function - ionizing radiation strongly activates p53.
      supported_by: *id044
  - term:
      id: GO:0031571
      label: mitotic G1 DNA damage checkpoint signaling
    evidence_type: IMP
    original_reference_id: PMID:7958916
    review:
      summary: p53 mediates G1 DNA damage checkpoint signaling through p21 
        induction.
      action: ACCEPT
      reason: Core function - G1/S checkpoint is a major p53-mediated cell cycle
        control.
      supported_by: *id045
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:17317671
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:17317671
          supporting_text: 2007 Feb 22. The notch regulator MAML1 interacts with
            p53 and functions as a coactivator.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:19151705
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:19151705
          supporting_text: CHD8 suppresses p53-mediated apoptosis through 
            histone H1 recruitment during early embryogenesis.
  - term:
      id: GO:0008270
      label: zinc ion binding
    evidence_type: TAS
    original_reference_id: PMID:10065153
    review:
      summary: p53 DNA-binding domain coordinates a zinc ion essential for 
        structural stability.
      action: ACCEPT
      reason: Core structural feature - zinc coordination is essential for p53 
        DNA-binding domain structure.
      supported_by:
        - reference_id: TP53-deep-research
          supporting_text: DNA-Binding Domain contains critical zinc 
            coordination site
        - reference_id: PMID:10065153
          supporting_text: Covalent and noncovalent modifiers of the p53 
            protein.
  - term:
      id: GO:0016605
      label: PML body
    evidence_type: IDA
    original_reference_id: PMID:12006491
    review:
      summary: p53 localizes to PML nuclear bodies, which is important for its 
        post-translational modification and activation.
      action: ACCEPT
      reason: Important regulatory localization - p53 accumulates in PML bodies 
        during stress responses where it undergoes acetylation.
      supported_by: *id041
  - term:
      id: GO:0051087
      label: protein-folding chaperone binding
    evidence_type: IPI
    original_reference_id: PMID:18086682
    review:
      summary: p53 has chaperone binding activity related to its folding and 
        stability.
      action: KEEP_AS_NON_CORE
      reason: Non-core function related to protein folding and quality control.
      supported_by:
        - reference_id: PMID:18086682
          supporting_text: 2007 Dec 17. Hsp60 regulation of tumor cell 
            apoptosis.
  - term:
      id: GO:0003682
      label: chromatin binding
    evidence_type: IDA
    original_reference_id: PMID:17599062
    review:
      summary: p53 binds chromatin at p53 response elements in target gene 
        promoters.
      action: ACCEPT
      reason: Core molecular function - chromatin binding is essential for p53's
        transcriptional regulation.
      supported_by: *id021
  - term:
      id: GO:0097193
      label: intrinsic apoptotic signaling pathway
    evidence_type: TAS
    original_reference_id: PMID:16462759
    review:
      summary: p53 mediates intrinsic apoptotic signaling through mitochondrial 
        pathway activation.
      action: ACCEPT
      reason: Core function - intrinsic apoptotic signaling is a major p53 tumor
        suppressor mechanism.
      supported_by:
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: Apoptosis induction seems to be mediated either by 
            stimulation of BAX
        - reference_id: PMID:16462759
          supporting_text: Feb 6. Inhibition of Bax activity is crucial for the 
            antiapoptotic function of the human papillomavirus E6 oncoprotein.
  - term:
      id: GO:0005634
      label: nucleus
    evidence_type: IDA
    original_reference_id: PMID:16507995
    review:
      summary: p53 is primarily a nuclear transcription factor, with nuclear 
        localization essential for its function.
      action: ACCEPT
      reason: Core cellular component - p53's nuclear localization is essential 
        for its transcription factor activity. Contains nuclear localization 
        signals at residues 305-322 and 369-375.
      supported_by: *id006
  - term:
      id: GO:0008104
      label: intracellular protein localization
    evidence_type: IDA
    original_reference_id: PMID:16507995
    review:
      summary: p53 localization is regulated by nuclear import and export 
        mechanisms.
      action: ACCEPT
      reason: Core regulatory mechanism - nucleo-cytoplasmic shuttling regulates
        p53 activity.
      supported_by:
        - reference_id: TP53-deep-research
          supporting_text: 'Nuclear Localization Signals (NLS): Function: Nuclear
            import and localization'
        - reference_id: PMID:16507995
          supporting_text: Myosin VI is a mediator of the p53-dependent cell 
            survival pathway.
  - term:
      id: GO:0046982
      label: protein heterodimerization activity
    evidence_type: IPI
    original_reference_id: PMID:10837489
    review:
      summary: p53 binds to hetero-dimeric protein partners as part of its 
        regulatory function.
      action: ACCEPT
      reason: p53 forms heterodimers with various regulatory proteins.
      supported_by:
        - reference_id: PMID:10837489
          supporting_text: MCL-1S, a splicing variant of the antiapoptotic BCL-2
            family member MCL-1, encodes a proapoptotic protein possessing only 
            the BH3 domain.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:15735003
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:15735003
          supporting_text: p53CSV, a novel p53-inducible gene involved in the 
            p53-dependent cell-survival pathway.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:11861836
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:11861836
          supporting_text: Human immunodeficiency virus type 1 Nef binds to 
            tumor suppressor p53 and protects cells against p53-mediated 
            apoptosis.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:11080164
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:11080164
          supporting_text: Regulation of p53 activity in nuclear bodies by a 
            specific PML isoform.
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: IDA
    original_reference_id: PMID:11080164
    review:
      summary: p53 is a nuclear transcription factor that localizes to the 
        nucleoplasm.
      action: ACCEPT
      reason: Core cellular localization - p53 functions as a transcription 
        factor in the nucleoplasm.
      supported_by: *id017
  - term:
      id: GO:0016363
      label: nuclear matrix
    evidence_type: IDA
    original_reference_id: PMID:11080164
    review:
      summary: p53 regulates nuclear matrix organization.
      action: ACCEPT
      reason: p53 interacts with nuclear matrix components and influences 
        nuclear organization.
      supported_by:
        - reference_id: PMID:11080164
          supporting_text: Regulation of p53 activity in nuclear bodies by a 
            specific PML isoform.
  - term:
      id: GO:0065003
      label: protein-containing complex assembly
    evidence_type: IDA
    original_reference_id: PMID:12915590
    review:
      summary: p53 forms protein-containing complexes with transcriptional 
        machinery and regulators.
      action: ACCEPT
      reason: Core function - p53 assembles into functional complexes to 
        regulate transcription.
      supported_by:
        - reference_id: TP53-deep-research
          supporting_text: Complex formation with transcriptional co-activators 
            (CBP/p300, TFIID)
        - reference_id: PMID:12915590
          supporting_text: Aug 12. Cellular stress and DNA damage invoke 
            temporally distinct Mdm2, p53 and PML complexes and damage-specific 
            nuclear relocalization.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:11684014
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:11684014
          supporting_text: ASPP proteins specifically stimulate the apoptotic 
            function of p53.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:8675009
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:8675009
          supporting_text: The XPB and XPD DNA helicases are components of the 
            p53-mediated apoptosis pathway.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:10608892
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:10608892
          supporting_text: Stabilization of the MDM2 oncoprotein by interaction 
            with the structurally related MDMX protein.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:12750254
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:12750254
          supporting_text: p29ING4 and p28ING5 bind to p53 and p300, and enhance
            p53 activity.
  - term:
      id: GO:0051097
      label: negative regulation of helicase activity
    evidence_type: TAS
    original_reference_id: PMID:7663514
    review:
      summary: p53 inhibits helicase activity as part of DNA repair regulation.
      action: ACCEPT
      reason: p53 can regulate DNA repair processes including helicase activity 
        during damage response.
      supported_by:
        - reference_id: PMID:7663514
          supporting_text: p53 modulation of TFIIH-associated nucleotide 
            excision repair activity.
  - term:
      id: GO:0006289
      label: nucleotide-excision repair
    evidence_type: IMP
    original_reference_id: PMID:7663514
    review:
      summary: p53 promotes nucleotide-excision repair (NER) through 
        transcriptional regulation of XPC and DDB2.
      action: ACCEPT
      reason: Core DNA repair function - p53 regulates multiple DNA repair genes
        including those in the NER pathway.
      supported_by:
        - reference_id: TP53-deep-research
          supporting_text: 'DNA Repair: XPC: Nucleotide excision repair; DDB2: DNA
            damage-binding protein'
        - reference_id: PMID:7663514
          supporting_text: p53 modulation of TFIIH-associated nucleotide 
            excision repair activity.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:1465435
    review:
      summary: Generic 'protein binding' term provides no functional information
        about p53's specific protein-protein interactions with MDM2, TFIID, 
        CBP/p300, etc.
      action: REMOVE
      reason: The term 'protein binding' is uninformative and should be removed 
        per GO curation guidelines. p53 has many well-characterized functional 
        protein interactions (MDM2, CBP/p300, TFIID, etc.) that are better 
        represented by more specific terms.
      supported_by:
        - reference_id: PMID:1465435
          supporting_text: Wild-type p53 binds to the TATA-binding protein and 
            represses transcription.
  - term:
      id: GO:0005739
      label: mitochondrion
    evidence_type: IDA
    original_reference_id: PMID:12667443
    review:
      summary: p53 translocates to mitochondria to directly promote apoptosis 
        through BAX activation.
      action: ACCEPT
      reason: Important localization for non-transcriptional apoptosis - p53 can
        directly induce MOMP at mitochondria.
      supported_by: *id029
  - term:
      id: GO:0005507
      label: copper ion binding
    evidence_type: IDA
    original_reference_id: PMID:7824276
    review:
      summary: p53 DNA-binding domain binds copper ions which can affect its 
        function.
      action: ACCEPT
      reason: Structural feature - copper binding can affect p53 structure and 
        function.
      supported_by:
        - reference_id: PMID:7824276
          supporting_text: 'Modulation by copper of p53 conformation and sequence-specific
            DNA binding: role for Cu(II)/Cu(I) redox mechanism.'
  - term:
      id: GO:0030308
      label: negative regulation of cell growth
    evidence_type: IMP
    original_reference_id: PMID:8986812
    review:
      summary: p53 negatively regulates cell growth as a fundamental tumor 
        suppressor mechanism.
      action: ACCEPT
      reason: Core function - growth inhibition is a primary tumor suppressor 
        activity of p53.
      supported_by:
        - reference_id: file:human/TP53/TP53-uniprot.txt
          supporting_text: Negatively regulates cell division by controlling 
            expression of a set of genes required for this process
        - reference_id: PMID:8986812
          supporting_text: Identification of a novel p53 functional domain that 
            is necessary for efficient growth suppression.
  - term:
      id: GO:0005634
      label: nucleus
    evidence_type: IDA
    original_reference_id: PMID:7720704
    review:
      summary: p53 is primarily a nuclear transcription factor, with nuclear 
        localization essential for its function.
      action: ACCEPT
      reason: Core cellular component - p53's nuclear localization is essential 
        for its transcription factor activity. Contains nuclear localization 
        signals at residues 305-322 and 369-375.
      supported_by: *id006
  - term:
      id: GO:0005737
      label: cytoplasm
    evidence_type: IDA
    original_reference_id: PMID:7720704
    review:
      summary: p53 localizes to cytoplasm as well as nucleus, with cytoplasmic 
        localization important for regulation.
      action: ACCEPT
      reason: Core cellular component - p53 shuttles between nucleus and 
        cytoplasm, with cytoplasmic localization important for MDM2-mediated 
        regulation.
      supported_by: *id020
  - term:
      id: GO:0003677
      label: DNA binding
    evidence_type: IMP
    original_reference_id: PMID:2144364
    review:
      summary: p53 binds DNA through its central DNA-binding domain (residues 
        102-292) to p53 response elements.
      action: ACCEPT
      reason: Core molecular function - DNA binding is essential for p53's 
        transcription factor activity.
      supported_by: *id032
  - term:
      id: GO:0003700
      label: DNA-binding transcription factor activity
    evidence_type: IDA
    original_reference_id: PMID:7587074
    review:
      summary: p53 is a well-characterized DNA-binding transcription factor that
        activates and represses target genes.
      action: ACCEPT
      reason: Core molecular function - p53 is one of the best-characterized 
        transcription factors, regulating >500 target genes.
      supported_by: *id015
  - term:
      id: GO:0005730
      label: nucleolus
    evidence_type: IDA
    original_reference_id: PMID:12080348
    review:
      summary: p53 localizes to nucleolus, particularly during stress responses 
        and ribosome biogenesis regulation.
      action: ACCEPT
      reason: p53 can localize to nucleolus and regulate ribosomal biogenesis as
        part of stress response.
      supported_by:
        - reference_id: PMID:12080348
          supporting_text: Nucleophosmin regulates the stability and 
            transcriptional activity of p53.
  - term:
      id: GO:0006355
      label: regulation of DNA-templated transcription
    evidence_type: IDA
    original_reference_id: PMID:7587074
    review:
      summary: p53 regulates DNA-templated transcription as both an activator 
        and repressor.
      action: ACCEPT
      reason: Core molecular function - transcription regulation is fundamental 
        to p53's tumor suppressor activity.
      supported_by: *id036
references:
  - id: PDB:3EXJ
    title: Crystal Structure of a p53 Core Tetramer Bound to DNA
    findings:
      - statement: p53 core tetramer forms symmetric contacts with DNA through 
          two p53 dimers
        supporting_text: The structure reveals that two p53DBD dimers bind to B 
          form DNA with no relative twist and that a p53 tetramer can bind to 
          DNA without introducing significant DNA bending
        full_text_unavailable: false
      - statement: p53 DNA binding involves conformational changes in loop L1 of
          two subunits
        supporting_text: In the absence of DNA, loop L1 of the p53 DNA binding 
          domain adopts an extended conformation, whereas two p53 subunits 
          switch to a recessed loop L1 conformation when bound to DNA as a 
          tetramer
        full_text_unavailable: false
      - statement: The structure demonstrates cooperative self-assembly of p53 
          on DNA response elements
        supporting_text: DNA binding by the p53 core domain is a cooperative 
          self-assembling process accompanied by structural changes of the p53 
          dimer and DNA
        full_text_unavailable: false
  - id: PDB:1C26
    title: Crystal Structure of p53 Tetramerization Domain
    findings:
      - statement: The tetramerization domain forms a dimer-of-dimers 
          architecture stabilizing the p53 tetramer
        supporting_text: The monomer consists of a beta strand and an alpha 
          helix, associates with a second monomer across an antiparallel beta 
          sheet and an antiparallel helix-helix interface to form a dimer
        full_text_unavailable: false
      - statement: Tetramerization domain structure reveals the molecular basis 
          for p53 oligomerization
        supporting_text: Crystal structure of p53 tetramerization domain 
          (residues 325 to 356) at 1.7 angstrom resolution
        full_text_unavailable: false
  - id: PDB:3TS8
    title: Crystal structure of a multidomain human p53 tetramer bound to the 
      natural CDKN1A(p21) p53-response element
    findings:
      - statement: Full-length p53 tetramer structure shows both DNA-binding and
          tetramerization domains in context
        supporting_text: Crystal structure of a multidomain human p53 tetramer 
          encompassing both the DNA-binding and homo-oligomerization domains in 
          complex with the natural p53-response element present upstream of the 
          CDKN1A (p21) gene promoter
        full_text_unavailable: false
      - statement: The structure demonstrates p53 binding to natural response 
          elements in target gene promoters
        supporting_text: The structure demonstrates how p53 recognizes and binds
          to its natural response element in the CDKN1A (p21) gene, a key cell 
          cycle checkpoint target
        full_text_unavailable: false
  - id: GO_REF:0000002
    title: Gene Ontology annotation through association of InterPro records with
      GO terms.
    findings: []
  - id: GO_REF:0000107
    title: Automatic transfer of experimentally verified manual GO annotation 
      data to orthologs using Ensembl Compara.
    findings: []
  - id: GO_REF:0000120
    title: Combined Automated Annotation using Multiple IEA Methods.
    findings: []
  - id: PMID:10360174
    title: Mutations in human ARF exon 2 disrupt its nucleolar localization and 
      impair its ability to block nuclear export of MDM2 and p53.
    findings: []
  - id: PMID:10962037
    title: Overexpression of MYC causes p53-dependent G2 arrest of normal 
      fibroblasts.
    findings: []
  - id: PMID:14654789
    title: A member of the Pyrin family, IFI16, is a novel BRCA1-associated 
      protein involved in the p53-mediated apoptosis pathway.
    findings: []
  - id: PMID:14744935
    title: Endoplasmic reticulum stress induces p53 cytoplasmic localization and
      prevents p53-dependent apoptosis by a pathway involving glycogen synthase 
      kinase-3beta.
    findings: []
  - id: PMID:15710329
    title: Human MUC1 oncoprotein regulates p53-responsive gene transcription in
      the genotoxic stress response.
    findings: []
  - id: PMID:16061649
    title: Ckap2 regulates aneuploidy, cell cycling, and cell death in a 
      p53-dependent manner.
    findings: []
  - id: PMID:17599062
    title: CDIP, a novel pro-apoptotic gene, regulates TNFalpha-mediated 
      apoptosis in a p53-dependent manner.
    findings: []
  - id: PMID:17996705
    title: An acetylation switch in p53 mediates holo-TFIID recruitment.
    findings: []
  - id: PMID:30089260
    title: p53 Regulates the Expression of LRP1 and Apoptosis through a Stress 
      Intensity-Dependent MicroRNA Feedback Loop.
    findings: []
  - id: PMID:30514107
    title: 'CELF1/p53 axis: a sustained antiproliferative signal leading to villus
      atrophy under total parenteral nutrition.'
    findings: []
  - id: file:human/TP53/TP53-uniprot.txt
    title: UniProt entry for Human TP53
    findings: []
  - id: file:human/TP53/TP53-deep-research.md
    title: TP53 Comprehensive Deep Research
    findings: []
  - id: GO_REF:0000024
    title: Manual transfer of experimentally-verified manual GO annotation data 
      to orthologs by curator judgment of sequence similarity.
    findings: []
  - id: GO_REF:0000033
    title: Annotation inferences using phylogenetic trees
    findings: []
  - id: GO_REF:0000043
    title: Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword 
      mapping
    findings: []
  - id: GO_REF:0000044
    title: Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular 
      Location vocabulary mapping, accompanied by conservative changes to GO 
      terms applied by UniProt.
    findings: []
  - id: GO_REF:0000052
    title: Gene Ontology annotation based on curation of immunofluorescence data
    findings: []
  - id: GO_REF:0000113
    title: Gene Ontology annotation of human sequence-specific DNA binding 
      transcription factors (DbTFs) based on the TFClass database
    findings: []
  - id: GO_REF:0000117
    title: Electronic Gene Ontology annotations created by ARBA machine learning
      models
    findings: []
  - id: PMID:10065153
    title: Covalent and noncovalent modifiers of the p53 protein.
    findings: []
  - id: PMID:10196247
    title: Characterization of an E1A-CBP interaction defines a novel 
      transcriptional adapter motif (TRAM) in CBP/p300.
    findings: []
  - id: PMID:10226625
    title: The yeast two-hybrid system reveals no interaction between p73 alpha 
      and SV40 large T-antigen.
    findings: []
  - id: PMID:10329733
    title: p53 suppresses the activation of the Bcl-2 promoter by the Brn-3a POU
      family transcription factor.
    findings: []
  - id: PMID:10415337
    title: Identification of a novel gene encoding a p53-associated protein.
    findings: []
  - id: PMID:10518217
    title: A novel cofactor for p300 that regulates the p53 response.
    findings: []
  - id: PMID:10597287
    title: Molecular interactions between telomerase and the tumor suppressor 
      protein p53 in vitro.
    findings: []
  - id: PMID:10608892
    title: Stabilization of the MDM2 oncoprotein by interaction with the 
      structurally related MDMX protein.
    findings: []
  - id: PMID:10666337
    title: Human topoisomerase IIalpha and IIbeta interact with the C-terminal 
      region of p53.
    findings: []
  - id: PMID:10823891
    title: The Huntington's disease protein interacts with p53 and CREB-binding 
      protein and represses transcription.
    findings: []
  - id: PMID:10837489
    title: MCL-1S, a splicing variant of the antiapoptotic BCL-2 family member 
      MCL-1, encodes a proapoptotic protein possessing only the BH3 domain.
    findings: []
  - id: PMID:10876243
    title: Structure of the negative regulatory domain of p53 bound to 
      S100B(betabeta).
    findings: []
  - id: PMID:11080164
    title: Regulation of p53 activity in nuclear bodies by a specific PML 
      isoform.
    findings: []
  - id: PMID:11146555
    title: Functional interaction between p53 and the interferon-inducible 
      nucleoprotein IFI 16.
    findings: []
  - id: PMID:11178989
    title: MDM2 enhances the function of estrogen receptor alpha in human breast
      cancer cells.
    findings: []
  - id: PMID:11359905
    title: The corepressor mSin3a interacts with the proline-rich domain of p53 
      and protects p53 from proteasome-mediated degradation.
    findings: []
  - id: PMID:11388671
    title: A putative protein inhibitor of activated STAT (PIASy) interacts with
      p53 and inhibits p53-mediated transactivation but not apoptosis.
    findings: []
  - id: PMID:11427532
    title: p53 Modulates the exonuclease activity of Werner syndrome protein.
    findings: []
  - id: PMID:11546806
    title: Cloning and characterization of a p53-related protein kinase 
      expressed in interleukin-2-activated cytotoxic T-cells, epithelial tumor 
      cell lines, and the testes.
    findings: []
  - id: PMID:11672523
    title: hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase.
    findings: []
  - id: PMID:11684014
    title: ASPP proteins specifically stimulate the apoptotic function of p53.
    findings: []
  - id: PMID:11706030
    title: Differential effect of ik3-1/cables on p53- and p73-induced cell 
      death.
    findings: []
  - id: PMID:11781842
    title: The Bloom syndrome protein interacts and cooperates with p53 in 
      regulation of transcription and cell growth control.
    findings: []
  - id: PMID:11861836
    title: Human immunodeficiency virus type 1 Nef binds to tumor suppressor p53
      and protects cells against p53-mediated apoptosis.
    findings: []
  - id: PMID:11877378
    title: Structure of the 53BP1 BRCT region bound to p53 and its comparison to
      the Brca1 BRCT structure.
    findings: []
  - id: PMID:11923872
    title: Deubiquitination of p53 by HAUSP is an important pathway for p53 
      stabilization.
    findings: []
  - id: PMID:11950834
    title: SWI/SNF complex interacts with tumor suppressor p53 and is necessary 
      for the activation of p53-mediated transcription.
    findings: []
  - id: PMID:12006491
    title: Human SIR2 deacetylates p53 and antagonizes PML/p53-induced cellular 
      senescence.
    findings: []
  - id: PMID:12080348
    title: Nucleophosmin regulates the stability and transcriptional activity of
      p53.
    findings: []
  - id: PMID:12172011
    title: Chk2 regulates irradiation-induced, p53-mediated apoptosis in 
      Drosophila.
    findings: []
  - id: PMID:12203124
    title: The Brn-3a transcription factor inhibits the pro-apoptotic effect of 
      p53 and enhances cell cycle arrest by differentially regulating the 
      activity of the p53 target genes encoding Bax and p21(CIP1/Waf1).
    findings: []
  - id: PMID:12433990
    title: Distinct promoter elements mediate the co-operative effect of Brn-3a 
      and p53 on the p21 promoter and their antagonism on the Bax promoter.
    findings: []
  - id: PMID:12609999
    title: The activation domains, the proline-rich domain, and the C-terminal 
      basic domain in p53 are necessary for acetylation of histones on the 
      proximal p21 promoter and interaction with p300/CREB-binding protein.
    findings: []
  - id: PMID:12620801
    title: Requirement of E6AP and the features of human papillomavirus E6 
      necessary to support degradation of p53.
    findings: []
  - id: PMID:12667443
    title: p53 has a direct apoptogenic role at the mitochondria.
    findings: []
  - id: PMID:12692135
    title: P63 alpha mutations lead to aberrant splicing of keratinocyte growth 
      factor receptor in the Hay-Wells syndrome.
    findings: []
  - id: PMID:12702766
    title: Characterization of cells and gene-targeted mice deficient for the 
      p53-binding kinase homeodomain-interacting protein kinase 1 (HIPK1).
    findings: []
  - id: PMID:12730672
    title: Physical and functional interaction between HCV core protein and the 
      different p73 isoforms.
    findings: []
  - id: PMID:12750254
    title: p29ING4 and p28ING5 bind to p53 and p300, and enhance p53 activity.
    findings: []
  - id: PMID:12915590
    title: Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 
      and PML complexes and damage-specific nuclear relocalization.
    findings: []
  - id: PMID:14557665
    title: Adenovirus E1B 55-kilodalton oncoprotein binds to Daxx and eliminates
      enhancement of p53-dependent transcription by Daxx.
    findings: []
  - id: PMID:14627987
    title: PARP-1 binds E2F-1 independently of its DNA binding and catalytic 
      domains, and acts as a novel coactivator of E2F-1-mediated transcription 
      during re-entry of quiescent cells into S phase.
    findings: []
  - id: PMID:1465435
    title: Wild-type p53 binds to the TATA-binding protein and represses 
      transcription.
    findings: []
  - id: PMID:14759370
    title: Structural mechanism of the bromodomain of the coactivator CBP in p53
      transcriptional activation.
    findings: []
  - id: PMID:14963330
    title: Direct activation of Bax by p53 mediates mitochondrial membrane 
      permeabilization and apoptosis.
    findings: []
  - id: PMID:14985081
    title: Hepatitis C virus core protein interacts with p53-binding protein, 
      53BP2/Bbp/ASPP2, and inhibits p53-mediated apoptosis.
    findings: []
  - id: PMID:15044383
    title: Aprataxin, a novel protein that protects against genotoxic stress.
    findings: []
  - id: PMID:15053879
    title: 'Phosphorylation on Thr-55 by TAF1 mediates degradation of p53: a role
      for TAF1 in cell G1 progression.'
    findings: []
  - id: PMID:15068796
    title: C. elegans SGK-1 is the critical component in the Akt/PKB kinase 
      complex to control stress response and life span.
    findings: []
  - id: PMID:15133049
    title: NMR structure of the N-terminal domain of SUMO ligase PIAS1 and its 
      interaction with tumor suppressor p53 and A/T-rich DNA oligomers.
    findings: []
  - id: PMID:15144954
    title: Nucleolar protein NPM interacts with HDM2 and protects tumor 
      suppressor protein p53 from HDM2-mediated degradation.
    findings: []
  - id: PMID:15149599
    title: Telomere shortening triggers senescence of human cells through a 
      pathway involving ATM, p53, and p21(CIP1), but not p16(INK4a).
    findings: []
  - id: PMID:15205477
    title: Calorie restriction promotes mammalian cell survival by inducing the 
      SIRT1 deacetylase.
    findings: []
  - id: PMID:15310821
    title: Identification of ASK and clock-associated proteins as molecular 
      partners of LKP2 (LOV kelch protein 2) in Arabidopsis.
    findings: []
  - id: PMID:15314173
    title: Ribosomal protein L23 activates p53 by inhibiting MDM2 function in 
      response to ribosomal perturbation but not to translation inhibition.
    findings: []
  - id: PMID:15358771
    title: Hsp90 regulates the activity of wild type p53 under physiological and
      elevated temperatures.
    findings: []
  - id: PMID:15364927
    title: Negative regulation of p53 functions by Daxx and the involvement of 
      MDM2.
    findings: []
  - id: PMID:15525938
    title: Regulation of p53 activity through lysine methylation.
    findings: []
  - id: PMID:15542844
    title: p53 Stabilization and accumulation induced by human vaccinia-related 
      kinase 1.
    findings: []
  - id: PMID:15565177
    title: A novel mitochondrial protein DIP mediates E2F1-induced apoptosis 
      independently of p53.
    findings: []
  - id: PMID:15577914
    title: Regulation of cellular response to oncogenic and oxidative stress by 
      Seladin-1.
    findings: []
  - id: PMID:15580310
    title: '17beta-Estradiol upregulates and activates WOX1/WWOXv1 and WOX2/WWOXv2
      in vitro: potential role in cancerous progression of breast and prostate to
      a premetastatic state in vivo.'
    findings: []
  - id: PMID:15604276
    title: Adenoviral E1A targets Mdm4 to stabilize tumor suppressor p53.
    findings: []
  - id: PMID:15629713
    title: Binding of natively unfolded HIF-1alpha ODD domain to p53.
    findings: []
  - id: PMID:15660129
    title: 'The DEAD box protein p68: a novel transcriptional coactivator of the p53
      tumour suppressor.'
    findings: []
  - id: PMID:15687255
    title: A mechanism of ubiquitin-independent proteasomal degradation of the 
      tumor suppressors p53 and p73.
    findings: []
  - id: PMID:15705871
    title: Homeobox Msx1 interacts with p53 tumor suppressor and inhibits tumor 
      growth by inducing apoptosis.
    findings: []
  - id: PMID:15735003
    title: p53CSV, a novel p53-inducible gene involved in the p53-dependent 
      cell-survival pathway.
    findings: []
  - id: PMID:15735006
    title: p53 modulates RPA-dependent and RPA-independent WRN helicase 
      activity.
    findings: []
  - id: PMID:15782130
    title: BARD1 induces apoptosis by catalysing phosphorylation of p53 by 
      DNA-damage response kinase.
    findings: []
  - id: PMID:15855171
    title: Direct interaction of the N-terminal domain of focal adhesion kinase 
      with the N-terminal transactivation domain of p53.
    findings: []
  - id: PMID:15916963
    title: Loss of HAUSP-mediated deubiquitination contributes to DNA 
      damage-induced destabilization of Hdmx and Hdm2.
    findings: []
  - id: PMID:15960975
    title: Physical association and coordinate function of the H3 K4 
      methyltransferase MLL1 and the H4 K16 acetyltransferase MOF.
    findings: []
  - id: PMID:15989956
    title: ARF-BP1/Mule is a critical mediator of the ARF tumor suppressor.
    findings: []
  - id: PMID:16003391
    title: AtBAG6, a novel calmodulin-binding protein, induces programmed cell 
      death in yeast and plants.
    findings: []
  - id: PMID:16131611
    title: p53 isoforms can regulate p53 transcriptional activity.
    findings: []
  - id: PMID:16151013
    title: PUMA couples the nuclear and cytoplasmic proapoptotic function of 
      p53.
    findings: []
  - id: PMID:16169070
    title: 'A human protein-protein interaction network: a resource for annotating
      the proteome.'
    findings: []
  - id: PMID:16189514
    title: Towards a proteome-scale map of the human protein-protein interaction
      network.
    findings: []
  - id: PMID:16213212
    title: Regulation of p53 translation and induction after DNA damage by 
      ribosomal protein L26 and nucleolin.
    findings: []
  - id: PMID:16227626
    title: Physical interaction and mutual transrepression between 
      CCAAT/enhancer-binding protein beta and the p53 tumor suppressor.
    findings: []
  - id: PMID:16291740
    title: Charge modification at multiple C-terminal lysine residues regulates 
      p53 oligomerization and its nucleus-cytoplasm trafficking.
    findings: []
  - id: PMID:16319068
    title: Camptothecin induces nuclear export of prohibitin preferentially in 
      transformed cells through a CRM-1-dependent mechanism.
    findings: []
  - id: PMID:16322561
    title: NIR is a novel INHAT repressor that modulates the transcriptional 
      activity of p53.
    findings: []
  - id: PMID:16376338
    title: Protein kinase A phosphorylates and regulates dimerization of 14-3-3 
      epsilon.
    findings: []
  - id: PMID:16376884
    title: 'Characterisation of the interface between nucleophosmin (NPM) and p53:
      potential role in p53 stabilisation.'
    findings: []
  - id: PMID:16377624
    title: Protein kinase C delta regulates Ser46 phosphorylation of p53 tumor 
      suppressor in the apoptotic response to DNA damage.
    findings: []
  - id: PMID:16402859
    title: 'Structural basis of competitive recognition of p53 and MDM2 by HAUSP/USP7:
      implications for the regulation of the p53-MDM2 pathway.'
    findings: []
  - id: PMID:16415881
    title: Structural basis for the methylation site specificity of SET7/9.
    findings: []
  - id: PMID:16432196
    title: The central region of HDM2 provides a second binding site for p53.
    findings: []
  - id: PMID:16442532
    title: Interaction of metallothionein with tumor suppressor p53 protein.
    findings: []
  - id: PMID:16443602
    title: WT p53, but not tumor-derived mutants, bind to Bcl2 via the DNA 
      binding domain and induce mitochondrial permeabilization.
    findings: []
  - id: PMID:16461914
    title: Core domain interactions in full-length p53 in solution.
    findings: []
  - id: PMID:16462759
    title: Inhibition of Bax activity is crucial for the antiapoptotic function 
      of the human papillomavirus E6 oncoprotein.
    findings: []
  - id: PMID:16474402
    title: Molecular recognition of p53 and MDM2 by USP7/HAUSP.
    findings: []
  - id: PMID:16479015
    title: Interferon-inducible protein IFIXalpha1 functions as a negative 
      regulator of HDM2.
    findings: []
  - id: PMID:16492744
    title: Mdm4 and Mdm2 cooperate to inhibit p53 activity in proliferating and 
      quiescent cells in vivo.
    findings: []
  - id: PMID:16493710
    title: Surface plasmon resonance imaging protein arrays for analysis of 
      triple protein interactions of HPV, E6, E6AP, and p53.
    findings: []
  - id: PMID:16507995
    title: Myosin VI is a mediator of the p53-dependent cell survival pathway.
    findings: []
  - id: PMID:16511572
    title: 14-3-3gamma binds to MDMX that is phosphorylated by UV-activated 
      Chk1, resulting in p53 activation.
    findings: []
  - id: PMID:16601686
    title: Tip60 and p400 are both required for UV-induced apoptosis but play 
      antagonistic roles in cell cycle progression.
    findings: []
  - id: PMID:16611888
    title: Targeting of p300/CREB binding protein coactivators by simian virus 
      40 is mediated through p53.
    findings: []
  - id: PMID:16616141
    title: Evidence that low doses of Taxol enhance the functional 
      transactivatory properties of p53 on p21 waf promoter in MCF-7 breast 
      cancer cells.
    findings: []
  - id: PMID:16690937
    title: NS3 protein of Hepatitis C virus associates with the tumour 
      suppressor p53 and inhibits its function in an NS3 sequence-dependent 
      manner.
    findings: []
  - id: PMID:16713569
    title: A protein-protein interaction network for human inherited ataxias and
      disorders of Purkinje cell degeneration.
    findings: []
  - id: PMID:16732283
    title: Lysine activation and functional analysis of E2-mediated conjugation 
      in the SUMO pathway.
    findings: []
  - id: PMID:16753148
    title: Polo-like kinase 1 regulates mitotic arrest after UV irradiation 
      through dephosphorylation of p53 and inducing p53 degradation.
    findings: []
  - id: PMID:16793543
    title: 'Structure of the Tfb1/p53 complex: Insights into the interaction between
      the p62/Tfb1 subunit of TFIIH and the activation domain of p53.'
    findings: []
  - id: PMID:16845383
    title: Critical role for Daxx in regulating Mdm2.
    findings: []
  - id: PMID:16847267
    title: MAGE-A tumor antigens target p53 transactivation function through 
      histone deacetylase recruitment and confer resistance to chemotherapeutic 
      agents.
    findings: []
  - id: PMID:16951253
    title: 'Crystal structure of SV40 large T-antigen bound to p53: interplay between
      a viral oncoprotein and a cellular tumor suppressor.'
    findings: []
  - id: PMID:16959611
    title: 'Gain of function of mutant p53: the mutant p53/NF-Y protein complex reveals
      an aberrant transcriptional mechanism of cell cycle regulation.'
    findings: []
  - id: PMID:17080083
    title: Inactivation of the p53 pathway in retinoblastoma.
    findings: []
  - id: PMID:17098746
    title: FBXO11 promotes the Neddylation of p53 and inhibits its 
      transcriptional activity.
    findings: []
  - id: PMID:17108971
    title: Repression of p53 activity by Smyd2-mediated methylation.
    findings: []
  - id: PMID:17121812
    title: CARPs are ubiquitin ligases that promote MDM2-independent p53 and 
      phospho-p53ser20 degradation.
    findings: []
  - id: PMID:17139261
    title: p53 mediates the negative regulation of MDM2 by orphan receptor TR3.
    findings: []
  - id: PMID:17145718
    title: Brn-3b enhances the pro-apoptotic effects of p53 but not its 
      induction of cell cycle arrest by cooperating in trans-activation of bax 
      expression.
    findings: []
  - id: PMID:17146433
    title: RGC32, a novel p53-inducible gene, is located on centrosomes during 
      mitosis and results in G2/M arrest.
    findings: []
  - id: PMID:17159902
    title: An essential function of the extreme C-terminus of MDM2 can be 
      provided by MDMX.
    findings: []
  - id: PMID:17170702
    title: Cytoplasmic destruction of p53 by the endoplasmic reticulum-resident 
      ubiquitin ligase 'Synoviolin'.
    findings: []
  - id: PMID:17184779
    title: Identification of differential proteins in nasopharyngeal carcinoma 
      cells with p53 silence by proteome analysis.
    findings: []
  - id: PMID:17237821
    title: Transcription factor TAFII250 promotes Mdm2-dependent turnover of 
      p53.
    findings: []
  - id: PMID:17245430
    title: A specific PP2A regulatory subunit, B56gamma, mediates DNA 
      damage-induced dephosphorylation of p53 at Thr55.
    findings: []
  - id: PMID:17254968
    title: PRAK is essential for ras-induced senescence and tumor suppression.
    findings: []
  - id: PMID:17268548
    title: Monoubiquitylation promotes mitochondrial p53 translocation.
    findings: []
  - id: PMID:17274640
    title: A limited screen for protein interactions reveals new roles for 
      protein phosphatase 1 in cell cycle control and apoptosis.
    findings: []
  - id: PMID:17290220
    title: The deubiquitinating enzyme USP2a regulates the p53 pathway by 
      targeting Mdm2.
    findings: []
  - id: PMID:17298945
    title: The conserved CPH domains of Cul7 and PARC are protein-protein 
      interaction modules that bind the tetramerization domain of p53.
    findings: []
  - id: PMID:17310983
    title: 'Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding
      to MDM2, stabilization of p53 protein, and activation of p53 function.'
    findings: []
  - id: PMID:17317671
    title: The notch regulator MAML1 interacts with p53 and functions as a 
      coactivator.
    findings: []
  - id: PMID:17347673
    title: Stra13 is induced by genotoxic stress and regulates 
      ionizing-radiation-induced apoptosis.
    findings: []
  - id: PMID:17403783
    title: HLA-B-associated transcript 3 (Bat3)/Scythe is essential for 
      p300-mediated acetylation of p53.
    findings: []
  - id: PMID:17438265
    title: Four domains of p300 each bind tightly to a sequence spanning both 
      transactivation subdomains of p53.
    findings: []
  - id: PMID:17470788
    title: PACT is a negative regulator of p53 and essential for cell growth and
      embryonic development.
    findings: []
  - id: PMID:17482142
    title: Attenuation of DNA damage checkpoint by PBK, a novel mitotic kinase, 
      involves protein-protein interaction with tumor suppressor p53.
    findings: []
  - id: PMID:17568776
    title: Phosphorylation of Pirh2 by calmodulin-dependent kinase II impairs 
      its ability to ubiquitinate p53.
    findings: []
  - id: PMID:17612295
    title: Yeast split-ubiquitin-based cytosolic screening system to detect 
      interactions between transcriptionally active proteins.
    findings: []
  - id: PMID:17620598
    title: Quaternary structures of tumor suppressor p53 and a specific p53 DNA 
      complex.
    findings: []
  - id: PMID:17662718
    title: Protein-protein interactions among human lens acidic and basic 
      beta-crystallins.
    findings: []
  - id: PMID:17707234
    title: Modulation of p53 function by SET8-mediated methylation at lysine 
      382.
    findings: []
  - id: PMID:17719541
    title: Hzf Determines cell survival upon genotoxic stress by modulating p53 
      transactivation.
    findings: []
  - id: PMID:17719542
    title: hCAS/CSE1L associates with chromatin and regulates expression of 
      select p53 target genes.
    findings: []
  - id: PMID:17805299
    title: p53 is regulated by the lysine demethylase LSD1.
    findings: []
  - id: PMID:17875722
    title: Efficient p53 activation and apoptosis by simultaneous disruption of 
      binding to MDM2 and MDMX.
    findings: []
  - id: PMID:17904127
    title: SVH-B interacts directly with p53 and suppresses the transcriptional 
      activity of p53.
    findings: []
  - id: PMID:17906639
    title: The prolyl isomerase Pin1 orchestrates p53 acetylation and 
      dissociation from the apoptosis inhibitor iASPP.
    findings: []
  - id: PMID:17938203
    title: Protein kinase C delta induces transcription of the TP53 tumor 
      suppressor gene by controlling death-promoting factor Btf in the apoptotic
      response to DNA damage.
    findings: []
  - id: PMID:17964266
    title: Active regulator of SIRT1 cooperates with SIRT1 and facilitates 
      suppression of p53 activity.
    findings: []
  - id: PMID:18086682
    title: Hsp60 regulation of tumor cell apoptosis.
    findings: []
  - id: PMID:18087040
    title: Regulation of p53 tetramerization and nuclear export by ARC.
    findings: []
  - id: PMID:18172499
    title: NUMB controls p53 tumour suppressor activity.
    findings: []
  - id: PMID:18230339
    title: Activation of p53-dependent responses in tumor cells treated with a 
      PARC-interacting peptide.
    findings: []
  - id: PMID:18235501
    title: DBC1 is a negative regulator of SIRT1.
    findings: []
  - id: PMID:18235502
    title: Negative regulation of the deacetylase SIRT1 by DBC1.
    findings: []
  - id: PMID:18275817
    title: Biochemical and structural studies of ASPP proteins reveal 
      differential binding to p53, p63, and p73.
    findings: []
  - id: PMID:18309296
    title: Proteasome activator PA28 gamma regulates p53 by enhancing its 
      MDM2-mediated degradation.
    findings: []
  - id: PMID:18316739
    title: Temporal activation of p53 by a specific MDM2 inhibitor is 
      selectively toxic to tumors and leads to complete tumor growth inhibition.
    findings: []
  - id: PMID:18354501
    title: 'Structural insight into the TFIIE-TFIIH interaction: TFIIE and p53 share
      the binding region on TFIIH.'
    findings: []
  - id: PMID:18382127
    title: CARPs enhance p53 turnover by degrading 14-3-3sigma and stabilizing 
      MDM2.
    findings: []
  - id: PMID:18388957
    title: NAD(P)H quinone oxidoreductase 1 inhibits the proteasomal degradation
      of the tumour suppressor p33(ING1b).
    findings: []
  - id: PMID:18391200
    title: Structure of tumor suppressor p53 and its intrinsically disordered 
      N-terminal transactivation domain.
    findings: []
  - id: PMID:18485870
    title: Acetylation is indispensable for p53 activation.
    findings: []
  - id: PMID:18504427
    title: Twist and p53 reciprocally regulate target genes via direct 
      interaction.
    findings: []
  - id: PMID:18510931
    title: Ectodermal factor restricts mesoderm differentiation by inhibiting 
      p53.
    findings: []
  - id: PMID:18549481
    title: TATA binding protein associated factor 3 (TAF3) interacts with p53 
      and inhibits its function.
    findings: []
  - id: PMID:18566590
    title: The tumour suppressor RASSF1A promotes MDM2 self-ubiquitination by 
      disrupting the MDM2-DAXX-HAUSP complex.
    findings: []
  - id: PMID:18624398
    title: Protein interaction data set highlighted with human Ras-MAPK/PI3K 
      signaling pathways.
    findings: []
  - id: PMID:18656471
    title: Microtubule-associated protein 1B light chain (MAP1B-LC1) negatively 
      regulates the activity of tumor suppressor p53 in neuroblastoma cells.
    findings: []
  - id: PMID:18677113
    title: Structure of the human Mdmx protein bound to the p53 tumor suppressor
      transactivation domain.
    findings: []
  - id: PMID:18690848
    title: NUPR1 interacts with p53, transcriptionally regulates p21 and rescues
      breast epithelial cells from doxorubicin-induced genotoxic stress.
    findings: []
  - id: PMID:18695251
    title: AIMP2/p38, the scaffold for the multi-tRNA synthetase complex, 
      responds to genotoxic stresses via p53.
    findings: []
  - id: PMID:18756595
    title: Prognostic significance of BMP and activin membrane-bound inhibitor 
      in colorectal cancer.
    findings: []
  - id: PMID:18812399
    title: 14-3-3 activation of DNA binding of p53 by enhancing its association 
      into tetramers.
    findings: []
  - id: PMID:18952844
    title: Inhibition of Thr-55 phosphorylation restores p53 nuclear 
      localization and sensitizes cancer cells to DNA damage.
    findings: []
  - id: PMID:18977328
    title: Inactivation of the CYLD deubiquitinase by HPV E6 mediates 
      hypoxia-induced NF-kappaB activation.
    findings: []
  - id: PMID:19008854
    title: Interferon-inducible protein, P56, inhibits HPV DNA replication by 
      binding to the viral protein E1.
    findings: []
  - id: PMID:19011621
    title: Arginine methylation regulates the p53 response.
    findings: []
  - id: PMID:19043414
    title: Molecular basis of Pirh2-mediated p53 ubiquitylation.
    findings: []
  - id: PMID:19098711
    title: RYBP stabilizes p53 by modulating MDM2.
    findings: []
  - id: PMID:19151705
    title: CHD8 suppresses p53-mediated apoptosis through histone H1 recruitment
      during early embryogenesis.
    findings: []
  - id: PMID:19160485
    title: A ribosomal protein L23-nucleophosmin circuit coordinates Mizl 
      function with cell growth.
    findings: []
  - id: PMID:19166840
    title: FKBP25, a novel regulator of the p53 pathway, induces the degradation
      of MDM2 and activation of p53.
    findings: []
  - id: PMID:19196987
    title: Phosphorylation of p53 by IkappaB kinase 2 promotes its degradation 
      by beta-TrCP.
    findings: []
  - id: PMID:19217391
    title: Structural basis for p300 Taz2-p53 TAD1 binding and modulation by 
      phosphorylation.
    findings: []
  - id: PMID:19234109
    title: Induction of SOX4 by DNA damage is critical for p53 stabilization and
      function.
    findings: []
  - id: PMID:19255450
    title: Structural basis for high-affinity peptide inhibition of p53 
      interactions with MDM2 and MDMX.
    findings: []
  - id: PMID:19339993
    title: Crosstalk between sumoylation and acetylation regulates p53-dependent
      chromatin transcription and DNA binding.
    findings: []
  - id: PMID:19345189
    title: A Mutant-p53/Smad complex opposes p63 to empower TGFbeta-induced 
      metastasis.
    findings: []
  - id: PMID:19411066
    title: Regulation of XIAP translation and induction by MDM2 following 
      irradiation.
    findings: []
  - id: PMID:19433796
    title: A tumor suppressive coactivator complex of p53 containing ASC-2 and 
      histone H3-lysine-4 methyltransferase MLL3 or its paralogue MLL4.
    findings: []
  - id: PMID:19483087
    title: Identification and characterization of two novel isoforms of Pirh2 
      ubiquitin ligase that negatively regulate p53 independent of RING finger 
      domains.
    findings: []
  - id: PMID:19505873
    title: Complementary quantitative proteomics reveals that transcription 
      factor AP-4 mediates E-box-dependent complex formation for transcriptional
      repression of HDM2.
    findings: []
  - id: PMID:19508870
    title: TOE1 interacts with p53 to modulate its transactivation potential.
    findings: []
  - id: PMID:19521340
    title: MDM4 (MDMX) localizes at the mitochondria and facilitates the 
      p53-mediated intrinsic-apoptotic pathway.
    findings: []
  - id: PMID:19536131
    title: Differential regulation of p53 and p21 by MKRN1 E3 ligase controls 
      cell cycle arrest and apoptosis.
    findings: []
  - id: PMID:19556538
    title: Trim24 targets endogenous p53 for degradation.
    findings: []
  - id: PMID:19619542
    title: Mdmx enhances p53 ubiquitination by altering the substrate preference
      of the Mdm2 ubiquitin ligase.
    findings: []
  - id: PMID:19626115
    title: Modulation of microRNA processing by p53.
    findings: []
  - id: PMID:19651603
    title: Structural basis for subversion of cellular control mechanisms by the
      adenoviral E1A oncoprotein.
    findings: []
  - id: PMID:19656744
    title: 'Ribosomal protein S3: A multi-functional protein that interacts with both
      p53 and MDM2 through its KH domain.'
    findings: []
  - id: PMID:19667193
    title: Ultraslow oligomerization equilibria of p53 and its implications.
    findings: []
  - id: PMID:19680552
    title: CK2 is the regulator of SIRT1 substrate-binding affinity, deacetylase
      activity and cellular response to DNA-damage.
    findings: []
  - id: PMID:19684601
    title: A molecular basis for phosphorylation-dependent SUMO conjugation by 
      the E2 UBC9.
    findings: []
  - id: PMID:19740107
    title: Epidermal growth factor receptor ligands as new extracellular targets
      for the metastasis-promoting S100A4 protein.
    findings: []
  - id: PMID:19749791
    title: Repression of SHP-1 expression by p53 leads to trkA tyrosine 
      phosphorylation and suppression of breast cancer cell proliferation.
    findings: []
  - id: PMID:19798103
    title: Efficient protection and isolation of ubiquitylated proteins using 
      tandem ubiquitin-binding entities.
    findings: []
  - id: PMID:19805293
    title: CBP and p300 are cytoplasmic E4 polyubiquitin ligases for p53.
    findings: []
  - id: PMID:19833129
    title: Polo-like kinase-1 phosphorylates MDM2 at Ser260 and stimulates 
      MDM2-mediated p53 turnover.
    findings: []
  - id: PMID:19857493
    title: 'Role of p53/FAK association and p53Ser46 phosphorylation in staurosporine-mediated
      apoptosis: wild type versus mutant p53-R175H.'
    findings: []
  - id: PMID:19933256
    title: Mechanistic differences in the transcriptional activation of p53 by 
      14-3-3 isoforms.
    findings: []
  - id: PMID:19951988
    title: WNT16B is a new marker of cellular senescence that regulates p53 
      activity and the phosphoinositide 3-kinase/AKT pathway.
    findings: []
  - id: PMID:20004160
    title: Averaging of electron subtomograms and random conical tilt 
      reconstructions through likelihood optimization.
    findings: []
  - id: PMID:20075864
    title: Coordinated regulation of p53 apoptotic targets BAX and PUMA by SMAR1
      through an identical MAR element.
    findings: []
  - id: PMID:20096447
    title: USP10 regulates p53 localization and stability by deubiquitinating 
      p53.
    findings: []
  - id: PMID:20118233
    title: G9a and Glp methylate lysine 373 in the tumor suppressor p53.
    findings: []
  - id: PMID:20123734
    title: NIR, an inhibitor of histone acetyltransferases, regulates 
      transcription factor TAp63 and is controlled by the cell cycle.
    findings: []
  - id: PMID:20124405
    title: G-protein-coupled receptor kinase 5 phosphorylates p53 and inhibits 
      DNA damage-induced apoptosis.
    findings: []
  - id: PMID:20134482
    title: Skp2B attenuates p53 function by inhibiting prohibitin.
    findings: []
  - id: PMID:20153329
    title: Reconstitution of the mitochondrial Hsp70 (mortalin)-p53 interaction 
      using purified proteins--identification of additional interacting regions.
    findings: []
  - id: PMID:20159018
    title: Identification and characterization of the novel protein CCDC106 that
      interacts with p53 and promotes its degradation.
    findings: []
  - id: PMID:20159469
    title: Crystal structure of the p53 core domain bound to a full consensus 
      site as a self-assembled tetramer.
    findings: []
  - id: PMID:20160708
    title: Feedback between p21 and reactive oxygen production is necessary for 
      cell senescence.
    findings: []
  - id: PMID:20167603
    title: DYRK1A and DYRK3 promote cell survival through phosphorylation and 
      activation of SIRT1.
    findings: []
  - id: PMID:20173098
    title: RFWD3-Mdm2 ubiquitin ligase complex positively regulates p53 
      stability in response to DNA damage.
    findings: []
  - id: PMID:20206173
    title: 'Structure of the p53 C-terminus bound to 14-3-3: implications for stabilization
      of the p53 tetramer.'
    findings: []
  - id: PMID:20227041
    title: Modulation of the vitamin D3 response by cancer-associated mutant 
      p53.
    findings: []
  - id: PMID:20228809
    title: BRD7 is a candidate tumour suppressor gene required for p53 function.
    findings: []
  - id: PMID:20332243
    title: MicroRNA145 targets BNIP3 and suppresses prostate cancer progression.
    findings: []
  - id: PMID:20364130
    title: Diversity in DNA recognition by p53 revealed by crystal structures 
      with Hoogsteen base pairs.
    findings: []
  - id: PMID:20378837
    title: Glutaminase 2, a novel p53 target gene regulating energy metabolism 
      and antioxidant function.
    findings: []
  - id: PMID:20385133
    title: p53 inhibits tumor cell invasion via the degradation of snail protein
      in hepatocellular carcinoma.
    findings: []
  - id: PMID:20421506
    title: Mapping the physical and functional interactions between the tumor 
      suppressors p53 and BRCA2.
    findings: []
  - id: PMID:20452352
    title: A novel hPirh2 splicing variant without ubiquitin protein ligase 
      activity interacts with p53 and is down-regulated in hepatocellular 
      carcinoma.
    findings: []
  - id: PMID:20515689
    title: MDM4 binds ligands via a mechanism in which disordered regions become
      structured.
    findings: []
  - id: PMID:20534433
    title: Synaptonemal complex formation and meiotic checkpoint signaling are 
      linked to the lateral element protein Red1.
    findings: []
  - id: PMID:20546595
    title: High-throughput sequencing identifies STAT3 as the DNA-associated 
      factor for p53-NF-kappaB-complex-dependent gene expression in human heart 
      failure.
    findings: []
  - id: PMID:20562916
    title: Dual-specificity phosphatase 26 is a novel p53 phosphatase and 
      inhibits p53 tumor suppressor functions in human neuroblastoma.
    findings: []
  - id: PMID:20591429
    title: S100 proteins interact with the N-terminal domain of MDM2.
    findings: []
  - id: PMID:20622899
    title: PBK/TOPK interacts with the DBD domain of tumor suppressor p53 and 
      modulates expression of transcriptional targets including p21.
    findings: []
  - id: PMID:20660729
    title: Polybromo-associated BRG1-associated factor components BRD7 and 
      BAF180 are critical regulators of p53 required for induction of 
      replicative senescence.
    findings: []
  - id: PMID:20705607
    title: Turning the RING domain protein MdmX into an active ubiquitin-protein
      ligase.
    findings: []
  - id: PMID:20708156
    title: Phosphorylation by casein kinase I promotes the turnover of the Mdm2 
      oncoprotein via the SCF(beta-TRCP) ubiquitin ligase.
    findings: []
  - id: PMID:20713054
    title: ARF-dependent regulation of ATM and p53 associated KZNF (Apak) 
      protein activity in response to oncogenic stress.
    findings: []
  - id: PMID:20725088
    title: Primate-specific RFPL1 gene controls cell-cycle progression through 
      cyclin B1/Cdc2 degradation.
    findings: []
  - id: PMID:20810912
    title: Hypoxia downregulates p53 but induces apoptosis and enhances 
      expression of BAD in cultures of human syncytiotrophoblasts.
    findings: []
  - id: PMID:20864041
    title: MAGE-RING protein complexes comprise a family of E3 ubiquitin 
      ligases.
    findings: []
  - id: PMID:20959462
    title: Aurora B interacts with NIR-p53, leading to p53 phosphorylation in 
      its DNA-binding domain and subsequent functional suppression.
    findings: []
  - id: PMID:21057547
    title: AXIN is an essential co-activator for the promyelocytic leukemia 
      protein in p53 activation.
    findings: []
  - id: PMID:21078964
    title: p53-mediated apoptosis requires inositol hexakisphosphate kinase-2.
    findings: []
  - id: PMID:21081126
    title: Anti-apoptotic protein TCTP controls the stability of the tumor 
      suppressor p53.
    findings: []
  - id: PMID:21130767
    title: SAFB1 interacts with and suppresses the transcriptional activity of 
      p53.
    findings: []
  - id: PMID:21132010
    title: GNL3L depletion destabilizes MDM2 and induces p53-dependent G2/M 
      arrest.
    findings: []
  - id: PMID:21170034
    title: TSPYL5 suppresses p53 levels and function by physical interaction 
      with USP7.
    findings: []
  - id: PMID:21170087
    title: Ribosomal protein S27-like and S27 interplay with p53-MDM2 axis as a 
      target, a substrate and a regulator.
    findings: []
  - id: PMID:21178074
    title: Electron microscopy studies on the quaternary structure of p53 reveal
      different binding modes for p53 tetramers in complex with DNA.
    findings: []
  - id: PMID:21245319
    title: Methyltransferase Set7/9 regulates p53 activity by interacting with 
      Sirtuin 1 (SIRT1).
    findings: []
  - id: PMID:21317932
    title: 'A new role of NUAK1: directly phosphorylating p53 and regulating cell
      proliferation.'
    findings: []
  - id: PMID:21336310
    title: p53 regulates biosynthesis through direct inactivation of 
      glucose-6-phosphate dehydrogenase.
    findings: []
  - id: PMID:21390126
    title: Inactivating mutations of acetyltransferase genes in B-cell lymphoma.
    findings: []
  - id: PMID:21397192
    title: 'Interferon-inducible protein 16: insight into the interaction with tumor
      suppressor p53.'
    findings: []
  - id: PMID:21423215
    title: Camptothecin-induced downregulation of MLL5 contributes to the 
      activation of tumor suppressor p53.
    findings: []
  - id: PMID:2144364
    title: Transcriptional activation by wild-type but not transforming mutants 
      of the p53 anti-oncogene.
    findings: []
  - id: PMID:21460856
    title: ATM-mediated phosphorylation activates the tumor-suppressive function
      of B56Ξ³-PP2A.
    findings: []
  - id: PMID:21471221
    title: Novel nucleolar pathway connecting intracellular energy status with 
      p53 activation.
    findings: []
  - id: PMID:21513714
    title: The ASPP proteins complex and cooperate with p300 to modulate the 
      transcriptional activity of p53.
    findings: []
  - id: PMID:21522129
    title: An induced fit mechanism regulates p53 DNA binding kinetics to confer
      sequence specificity.
    findings: []
  - id: PMID:21597459
    title: E3 ubiquitin ligase Hades negatively regulates the exonuclear 
      function of p53.
    findings: []
  - id: PMID:21625211
    title: COP9 signalosome subunit 6 stabilizes COP1, which functions as an E3 
      ubiquitin ligase for 14-3-3Οƒ.
    findings: []
  - id: PMID:21653829
    title: Protein interactome reveals converging molecular pathways among 
      autism disorders.
    findings: []
  - id: PMID:21670263
    title: Structural and functional characterization of an atypical activation 
      domain in erythroid Kruppel-like factor (EKLF).
    findings: []
  - id: PMID:21726810
    title: Caspase-2-mediated cleavage of Mdm2 creates a p53-induced positive 
      feedback loop.
    findings: []
  - id: PMID:21741598
    title: A Pin1/mutant p53 axis promotes aggressiveness in breast cancer.
    findings: []
  - id: PMID:21782458
    title: Structural basis of substrate methylation and inhibition of SMYD2.
    findings: []
  - id: PMID:21821029
    title: Aurora-A phosphorylates hnRNPK and disrupts its interaction with p53.
    findings: []
  - id: PMID:21831840
    title: Wild-type p53 controls cell motility and invasion by dual regulation 
      of MET expression.
    findings: []
  - id: PMID:21857681
    title: A stress response pathway regulates DNA damage through 
      Ξ²2-adrenoreceptors and Ξ²-arrestin-1.
    findings: []
  - id: PMID:21892170
    title: Structural analysis of the interaction between Hsp90 and the tumor 
      suppressor protein p53.
    findings: []
  - id: PMID:21900206
    title: A directed protein interaction network for investigating 
      intracellular signal transduction.
    findings: []
  - id: PMID:21952639
    title: NIRF constitutes a nodal point in the cell cycle network and is a 
      candidate tumor suppressor.
    findings: []
  - id: PMID:21988832
    title: Toward an understanding of the protein interaction network of the 
      human liver.
    findings: []
  - id: PMID:22056774
    title: Bilateral inhibition of HAUSP deubiquitinase by a viral interferon 
      regulatory factor protein.
    findings: []
  - id: PMID:22085928
    title: Regulation of p53 stability and function by the deubiquitinating 
      enzyme USP42.
    findings: []
  - id: PMID:22103682
    title: Specific domains of nucleolin interact with Hdm2 and antagonize 
      Hdm2-mediated p53 ubiquitination.
    findings: []
  - id: PMID:22124327
    title: Positive regulation of p53 stability and activity by the 
      deubiquitinating enzyme Otubain 1.
    findings: []
  - id: PMID:22265415
    title: Mutant p53 disrupts mammary tissue architecture via the mevalonate 
      pathway.
    findings: []
  - id: PMID:22340593
    title: Aurora kinase-A inactivates DNA damage-induced apoptosis and spindle 
      assembly checkpoint response functions of p73.
    findings: []
  - id: PMID:22451927
    title: Role of the translationally controlled tumor protein in DNA damage 
      sensing and repair.
    findings: []
  - id: PMID:22499945
    title: Atg7 modulates p53 activity to regulate cell cycle and survival 
      during metabolic stress.
    findings: []
  - id: PMID:22499991
    title: Downregulation of endothelial microRNA-200b supports cutaneous wound 
      angiogenesis by desilencing GATA binding protein 2 and vascular 
      endothelial growth factor receptor 2.
    findings: []
  - id: PMID:22510990
    title: AKT-dependent phosphorylation of Niban regulates nucleophosmin- and 
      MDM2-mediated p53 stability and cell apoptosis.
    findings: []
  - id: PMID:22521434
    title: Hepatocyte growth factor (HGF) and 1,25-dihydroxyvitamin D together 
      stimulate human bone marrow-derived stem cells toward the osteogenic 
      phenotype by HGF-induced up-regulation of VDR.
    findings: []
  - id: PMID:22522597
    title: Nucleolar protein GLTSCR2 stabilizes p53 in response to ribosomal 
      stresses.
    findings: []
  - id: PMID:22574148
    title: p53 Activation following Rift Valley fever virus infection 
      contributes to cell death and viral production.
    findings: []
  - id: PMID:22575647
    title: An autoregulatory feedback loop between Mdm2 and SHP that fine tunes 
      Mdm2 and SHP stability.
    findings: []
  - id: PMID:22578566
    title: Oncosuppressive role of p53-induced miR-205 in triple negative breast
      cancer.
    findings: []
  - id: PMID:22653443
    title: Mutant p53 interactome identifies nardilysin as a p53R273H-specific 
      binding partner that promotes invasion.
    findings: []
  - id: PMID:22659184
    title: Mutational analysis reveals a dual role of Mdm2 acidic domain in the 
      regulation of p53 stability.
    findings: []
  - id: PMID:22723347
    title: Differentiated embryo-chondrocyte expressed gene 1 regulates 
      p53-dependent cell survival versus cell death through macrophage 
      inhibitory cytokine-1.
    findings: []
  - id: PMID:22726440
    title: p53 opens the mitochondrial permeability transition pore to trigger 
      necrosis.
    findings: []
  - id: PMID:22783376
    title: Induction of apoptosis by cytoplasmically localized wild-type p53 and
      the S121F mutant super p53.
    findings: []
  - id: PMID:22810585
    title: Viral immune modulators perturb the human molecular network by common
      and unique strategies.
    findings: []
  - id: PMID:22810586
    title: Interpreting cancer genomes using systematic host network 
      perturbations by tumour virus proteins.
    findings: []
  - id: PMID:22819825
    title: TRIAD1 inhibits MDM2-mediated p53 ubiquitination and degradation.
    findings: []
  - id: PMID:22869143
    title: BMK1 is involved in the regulation of p53 through disrupting the 
      PML-MDM2 interaction.
    findings: []
  - id: PMID:22945289
    title: ZNF385B is characteristically expressed in germinal center B cells 
      and involved in B-cell apoptosis.
    findings: []
  - id: PMID:22972749
    title: Domain-domain interactions in full-length p53 and a specific DNA 
      complex probed by methyl NMR spectroscopy.
    findings: []
  - id: PMID:22975381
    title: 'A "twist box" code of p53 inactivation: twist box: p53 interaction promotes
      p53 degradation.'
    findings: []
  - id: PMID:23010591
    title: Structural features of human histone acetyltransferase p300 and its 
      complex with p53.
    findings: []
  - id: PMID:23027130
    title: TrkC signaling is activated in adenoid cystic carcinoma and requires 
      NT-3 to stimulate invasive behavior.
    findings: []
  - id: PMID:23063560
    title: HMGB1-facilitated p53 DNA binding occurs via HMG-Box/p53 
      transactivation domain interaction, regulated by the acidic tail.
    findings: []
  - id: PMID:23092970
    title: SUMOylation of hnRNP-K is required for p53-mediated cell-cycle arrest
      in response to DNA damage.
    findings: []
  - id: PMID:23320542
    title: Evidence for self-association of the alternative sigma factor Οƒ54.
    findings: []
  - id: PMID:23329847
    title: Regulation of the cyclin-dependent kinase inhibitor 1A gene (CDKN1A) 
      by the repressor BOZF1 through inhibition of p53 acetylation and 
      transcription factor Sp1 binding.
    findings: []
  - id: PMID:23431171
    title: MOZ increases p53 acetylation and premature senescence through its 
      complex formation with PML.
    findings: []
  - id: PMID:23576507
    title: Rotavirus-encoded nonstructural protein 1 modulates cellular 
      apoptotic machinery by targeting tumor suppressor protein p53.
    findings: []
  - id: PMID:23623661
    title: Restoring p53 function in human melanoma cells by inhibiting MDM2 and
      cyclin B1/CDK1-phosphorylated nuclear iASPP.
    findings: []
  - id: PMID:23629966
    title: Deacetylation of p53 induces autophagy by suppressing Bmf expression.
    findings: []
  - id: PMID:23734815
    title: 'Fludarabine treatment favors the retention of miR-485-3p by prostate cancer
      cells: implications for survival.'
    findings: []
  - id: PMID:23752197
    title: S100A4 interacts with p53 in the nucleus and promotes p53 
      degradation.
    findings: []
  - id: PMID:23776060
    title: Identification of a second Nutlin-3 responsive interaction site in 
      the N-terminal domain of MDM2 using hydrogen/deuterium exchange mass 
      spectrometry.
    findings: []
  - id: PMID:23870121
    title: SET1 and p300 act synergistically, through coupled histone 
      modifications, in transcriptional activation by p53.
    findings: []
  - id: PMID:24051492
    title: p53 regulates Period2 expression and the circadian clock.
    findings: []
  - id: PMID:24101517
    title: Mitochondrial disulfide relay mediates translocation of p53 and 
      partitions its subcellular activity.
    findings: []
  - id: PMID:24207125
    title: Transient protein states in designing inhibitors of the MDM2-p53 
      interaction.
    findings: []
  - id: PMID:24219989
    title: Acetylation of p53 stimulates miRNA processing and determines cell 
      survival following genotoxic stress.
    findings: []
  - id: PMID:24289924
    title: Phosphorylation of p53 by TAF1 inactivates p53-dependent 
      transcription in the DNA damage response.
    findings: []
  - id: PMID:24356969
    title: Rbm24, an RNA-binding protein and a target of p53, regulates p21 
      expression via mRNA stability.
    findings: []
  - id: PMID:24380853
    title: The aberrant expression and localization of prohibitin during 
      apoptosis of human cholangiocarcinoma Mz-ChA-1 cells.
    findings: []
  - id: PMID:24449765
    title: 'Activation of p53 transcriptional activity by SMRT: a histone deacetylase
      3-independent function of a transcriptional corepressor.'
    findings: []
  - id: PMID:24492002
    title: VRK1 interacts with p53 forming a basal complex that is activated by 
      UV-induced DNA damage.
    findings: []
  - id: PMID:24625977
    title: Ubiquitin-like (UBX)-domain-containing protein, UBXN2A, promotes cell
      death by interfering with the p53-Mortalin interactions in colon cancer 
      cells.
    findings: []
  - id: PMID:24652652
    title: DRAGO (KIAA0247), a new DNA damage-responsive, p53-inducible gene 
      that cooperates with p53 as oncosuppressor. [Corrected].
    findings: []
  - id: PMID:24667498
    title: JMJD6 promotes colon carcinogenesis through negative regulation of 
      p53 by hydroxylation.
    findings: []
  - id: PMID:24722188
    title: Protein interaction network of alternatively spliced isoforms from 
      brain links genetic risk factors for autism.
    findings: []
  - id: PMID:24814347
    title: The DNA-binding domain mediates both nuclear and cytosolic functions 
      of p53.
    findings: []
  - id: PMID:25168243
    title: Cofactor Strap regulates oxidative phosphorylation and mitochondrial 
      p53 activity through ATP synthase.
    findings: []
  - id: PMID:25241761
    title: Using an in situ proximity ligation assay to systematically profile 
      endogenous protein-protein interactions in a pathway network.
    findings: []
  - id: PMID:25314079
    title: Acetylation of snail modulates the cytokinome of cancer cells to 
      enhance the recruitment of macrophages.
    findings: []
  - id: PMID:25384516
    title: Rad54B serves as a scaffold in the DNA damage response that limits 
      checkpoint strength.
    findings: []
  - id: PMID:25402006
    title: In silico prediction of physical protein interactions and 
      characterization of interactome orphans.
    findings: []
  - id: PMID:25417702
    title: Functional interplay between MDM2, p63/p73 and mutant p53.
    findings: []
  - id: PMID:25422469
    title: Disruption of FAT10-MAD2 binding inhibits tumor progression.
    findings: []
  - id: PMID:25502805
    title: A massively parallel pipeline to clone DNA variants and examine 
      molecular phenotypes of human disease mutations.
    findings: []
  - id: PMID:25579814
    title: Structural plasticity of methyllysine recognition by the tandem tudor
      domain of 53BP1.
    findings: []
  - id: PMID:25591766
    title: RNF125 is a ubiquitin-protein ligase that promotes p53 degradation.
    findings: []
  - id: PMID:25609649
    title: Proteomic analyses reveal distinct chromatin-associated and soluble 
      transcription factor complexes.
    findings: []
  - id: PMID:25651062
    title: An acetyl-methyl switch drives a conformational change in p53.
    findings: []
  - id: PMID:25670079
    title: 14-3-3ΞΆ turns TGF-Ξ²'s function from tumor suppressor to metastasis 
      promoter in breast cancer by contextual changes of Smad partners from p53 
      to Gli2.
    findings: []
  - id: PMID:25837623
    title: Regulation of protein quality control by UBE4B and LSD1 through 
      p53-mediated transcription.
    findings: []
  - id: PMID:25857266
    title: Pontin, a new mutant p53-binding protein, promotes gain-of-function 
      of mutant p53.
    findings: []
  - id: PMID:26100857
    title: A metabolic stress-inducible miR-34a-HNF4Ξ± pathway regulates lipid 
      and lipoprotein metabolism.
    findings: []
  - id: PMID:26302407
    title: Combined CSL and p53 downregulation promotes cancer-associated 
      fibroblast activation.
    findings: []
  - id: PMID:26331536
    title: Gain-of-function p53 mutants co-opt chromatin pathways to drive 
      cancer growth.
    findings: []
  - id: PMID:26334721
    title: Jmjd5 functions as a regulator of p53 signaling during mouse 
      embryogenesis.
    findings: []
  - id: PMID:26634371
    title: Structural studies of UBXN2A and mortalin interaction and the 
      putative role of silenced UBXN2A in preventing response to chemotherapy.
    findings: []
  - id: PMID:26789255
    title: Structure of the E6/E6AP/p53 complex required for HPV-mediated 
      degradation of p53.
    findings: []
  - id: PMID:27031958
    title: Nupr1/Chop signal axis is involved in mitochondrion-related 
      endothelial cell apoptosis induced by methamphetamine.
    findings: []
  - id: PMID:27107012
    title: Pooled-matrix protein interaction screens using Barcode Fusion 
      Genetics.
    findings: []
  - id: PMID:27323408
    title: LACE1 interacts with p53 and mediates its mitochondrial translocation
      and apoptosis.
    findings: []
  - id: PMID:27519799
    title: p53 down-regulates SARS coronavirus replication and is targeted by 
      the SARS-unique domain and PLpro via E3 ubiquitin ligase RCHY1.
    findings: []
  - id: PMID:27605672
    title: Mitogen-Activated Protein Kinase Kinase 2, a Novel E2-Interacting 
      Protein, Promotes the Growth of Classical Swine Fever Virus via 
      Attenuation of the JAK-STAT Signaling Pathway.
    findings: []
  - id: PMID:28842590
    title: DDX3 localizes to the centrosome and prevents multipolar mitosis by 
      epigenetically and translationally modulating p53 expression.
    findings: []
  - id: PMID:29187402
    title: The E3 Ligase RING1 Targets p53 for Degradation and Promotes Cancer 
      Cell Proliferation and Survival.
    findings: []
  - id: PMID:29340707
    title: VRK1 and AURKB form a complex that cross inhibit their kinase 
      activity and the phosphorylation of histone H3 in the progression of 
      mitosis.
    findings: []
  - id: PMID:29474172
    title: Haploinsufficiency of Trp53 dramatically extends the lifespan of 
      Sirt6-deficient mice.
    findings: []
  - id: PMID:29628311
    title: A Family of Vertebrate-Specific Polycombs Encoded by the LCOR/LCORL 
      Genes Balance PRC2 Subtype Activities.
    findings: []
  - id: PMID:29681526
    title: A Designed Peptide Targets Two Types of Modifications of p53 with 
      Anti-cancer Activity.
    findings: []
  - id: PMID:29997244
    title: 'LuTHy: a double-readout bioluminescence-based two-hybrid technology for
      quantitative mapping of protein-protein interactions in mammalian cells.'
    findings: []
  - id: PMID:30146126
    title: De Novo Mutations Activating Germline TP53 in an Inherited 
      Bone-Marrow-Failure Syndrome.
    findings: []
  - id: PMID:31467278
    title: Maximizing binary interactome mapping with a minimal number of 
      assays.
    findings: []
  - id: PMID:31511497
    title: miR-146a attenuates apoptosis and modulates autophagy by targeting 
      TAF9b/P53 pathway in doxorubicin-induced cardiotoxicity.
    findings: []
  - id: PMID:31515488
    title: Extensive disruption of protein interactions by genetic variants 
      across the allele frequency spectrum in human populations.
    findings: []
  - id: PMID:31837246
    title: High-throughput competitive fluorescence polarization assay reveals 
      functional redundancy in the S100 protein family.
    findings: []
  - id: PMID:31953488
    title: Liquid-like droplet formation by tumor suppressor p53 induced by 
      multivalent electrostatic interactions between two disordered domains.
    findings: []
  - id: PMID:32606738
    title: P55PIK Regulates P53-Dependent Apoptosis in Cancer Cells by 
      Interacting with P53 DNA-Specific Domain.
    findings: []
  - id: PMID:32814053
    title: Interactome Mapping Provides a Network of Neurodegenerative Disease 
      Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.
    findings: []
  - id: PMID:33591310
    title: DAZAP2 acts as specifier of the p53 response to DNA damage.
    findings: []
  - id: PMID:33961781
    title: Dual proteome-scale networks reveal cell-specific remodeling of the 
      human interactome.
    findings: []
  - id: PMID:34316702
    title: Elucidation of the BMI1 interactome identifies novel regulatory roles
      in glioblastoma.
    findings: []
  - id: PMID:34381247
    title: The tumor suppressor folliculin inhibits lactate dehydrogenase A and 
      regulates the Warburg effect.
    findings: []
  - id: PMID:34404770
    title: ZNF768 links oncogenic RAS to cellular senescence.
    findings: []
  - id: PMID:34591612
    title: A protein interaction landscape of breast cancer.
    findings: []
  - id: PMID:34591642
    title: A protein network map of head and neck cancer reveals PIK3CA mutant 
      drug sensitivity.
    findings: []
  - id: PMID:35044719
    title: Proteome-scale mapping of binding sites in the unstructured regions 
      of the human proteome.
    findings: []
  - id: PMID:35122041
    title: Aldolase B suppresses hepatocellular carcinogenesis by inhibiting 
      G6PD and pentose phosphate pathways.
    findings: []
  - id: PMID:35140242
    title: Human transcription factor protein interaction networks.
    findings: []
  - id: PMID:35271311
    title: 'OpenCell: Endogenous tagging for the cartography of human cellular organization.'
    findings: []
  - id: PMID:35512704
    title: Systematic discovery of mutation-directed neo-protein-protein 
      interactions in cancer.
    findings: []
  - id: PMID:35618207
    title: Ser392 phosphorylation modulated a switch between p53 and 
      transcriptional condensates.
    findings: []
  - id: PMID:36108750
    title: Phase separation of p53 induced by its unstructured basic region and 
      prevented by oncogenic mutations in tetramerization domain.
    findings: []
  - id: PMID:36634798
    title: Phosphorylation and specific DNA improved the incorporation ability 
      of p53 into functional condensates.
    findings: []
  - id: PMID:36931259
    title: A central chaperone-like role for 14-3-3 proteins in human cells.
    findings: []
  - id: PMID:38653238
    title: Alanyl-tRNA synthetase, AARS1, is a lactate sensor and 
      lactyltransferase that lactylates p53 and contributes to tumorigenesis.
    findings: []
  - id: PMID:39009827
    title: Proteome-scale characterisation of motif-based interactome rewiring 
      by disease mutations.
    findings: []
  - id: PMID:7559631
    title: Transactivation ability of p53 transcriptional activation domain is 
      directly related to the binding affinity to TATA-binding protein.
    findings: []
  - id: PMID:7587074
    title: 'Targets for transcriptional activation by wild-type p53: endogenous retroviral
      LTR, immunoglobulin-like promoter, and an internal promoter of the mdm2 gene.'
    findings: []
  - id: PMID:7663514
    title: p53 modulation of TFIIH-associated nucleotide excision repair 
      activity.
    findings: []
  - id: PMID:7720704
    title: Direct involvement of p53 in programmed cell death of 
      oligodendrocytes.
    findings: []
  - id: PMID:7799929
    title: Two domains of p53 interact with the TATA-binding protein, and the 
      adenovirus 13S E1A protein disrupts the association, relieving 
      p53-mediated transcriptional repression.
    findings: []
  - id: PMID:7824276
    title: 'Modulation by copper of p53 conformation and sequence-specific DNA binding:
      role for Cu(II)/Cu(I) redox mechanism.'
    findings: []
  - id: PMID:7958916
    title: DNA damage triggers a prolonged p53-dependent G1 arrest and long-term
      induction of Cip1 in normal human fibroblasts.
    findings: []
  - id: PMID:8183576
    title: Human p53 directs DNA strand reassociation and is photolabelled by 
      8-azido ATP.
    findings: []
  - id: PMID:8207801
    title: Inhibition of p53 DNA binding by human papillomavirus E6 proteins.
    findings: []
  - id: PMID:8344494
    title: Identification of mutations in p53 that affect its binding to SV40 
      large T antigen by using the yeast two-hybrid system.
    findings: []
  - id: PMID:8675009
    title: The XPB and XPD DNA helicases are components of the p53-mediated 
      apoptosis pathway.
    findings: []
  - id: PMID:8875926
    title: Structure of the p53 tumor suppressor bound to the ankyrin and SH3 
      domains of 53BP2.
    findings: []
  - id: PMID:8875929
    title: Structure of the MDM2 oncoprotein bound to the p53 tumor suppressor 
      transactivation domain.
    findings: []
  - id: PMID:8986812
    title: Identification of a novel p53 functional domain that is necessary for
      efficient growth suppression.
    findings: []
  - id: PMID:9050995
    title: Identification of p53 unbound to T-antigen in human cells transformed
      by simian virus 40 T-antigen.
    findings: []
  - id: PMID:9054499
    title: Oncogenic ras provokes premature cell senescence associated with 
      accumulation of p53 and p16INK4a.
    findings: []
  - id: PMID:9188558
    title: Nuclear localization of the NS3 protein of hepatitis C virus and 
      factors affecting the localization.
    findings: []
  - id: PMID:9194564
    title: Synergistic activation of transcription by CBP and p53.
    findings: []
  - id: PMID:9194565
    title: Binding and modulation of p53 by p300/CBP coactivators.
    findings: []
  - id: PMID:9271120
    title: 'Repression of p53-mediated transcription by MDM2: a dual mechanism.'
    findings: []
  - id: PMID:9380510
    title: Interaction of p53 with the human Rad51 protein.
    findings: []
  - id: PMID:9472015
    title: High mobility group protein-1 (HMG-1) is a unique activator of p53.
    findings: []
  - id: PMID:9529249
    title: 'ARF promotes MDM2 degradation and stabilizes p53: ARF-INK4a locus deletion
      impairs both the Rb and p53 tumor suppression pathways.'
    findings: []
  - id: PMID:9733515
    title: Activation of the ATM kinase by ionizing radiation and 
      phosphorylation of p53.
    findings: []
  - id: PMID:9807817
    title: An Arabidopsis immunophilin, AtFKBP12, binds to AtFIP37 (FKBP 
      interacting protein) in an interaction that is disrupted by FK506.
    findings: []
  - id: PMID:9827557
    title: Complex formation of the nonstructural protein 3 of hepatitis C virus
      with the p53 tumor suppressor.
    findings: []
  - id: Reactome:R-HSA-264435
    title: Dissociation of the COP1-p53 complex
    findings: []
  - id: Reactome:R-HSA-2997706
    title: MDM2 SUMOylates TP53 with SUMO2,3
    findings: []
  - id: Reactome:R-HSA-3215152
    title: TP53 in complex with EP300, PRMT1 and CARM1 binds the GADD45A 
      promoter
    findings: []
  - id: Reactome:R-HSA-3215251
    title: TP53INP1 and HIPK2 bind TP53
    findings: []
  - id: Reactome:R-HSA-3215310
    title: USP7 deubiquitinates TP53 and counteracts MDM2
    findings: []
  - id: Reactome:R-HSA-3221982
    title: BANP binds TP53
    findings: []
  - id: Reactome:R-HSA-3222006
    title: STK11 (LKB1) phosphorylates NUAK1
    findings: []
  - id: Reactome:R-HSA-3222020
    title: NUAK1 phosphorylates TP53
    findings: []
  - id: Reactome:R-HSA-3222093
    title: BRD7 binds TP53 and EP300
    findings: []
  - id: Reactome:R-HSA-3222116
    title: L3MBTL1 binds TP53 monomethylated on K382
    findings: []
  - id: Reactome:R-HSA-3222128
    title: NOC2L binds TP53
    findings: []
  - id: Reactome:R-HSA-3222237
    title: SMYD2 methylates TP53
    findings: []
  - id: Reactome:R-HSA-3222259
    title: PHF20 binds TP53 dimethylated on K370 and K382
    findings: []
  - id: Reactome:R-HSA-3239014
    title: MAPKAPK5 phosphorylates TP53
    findings: []
  - id: Reactome:R-HSA-3700981
    title: TP53 family members bind the BAX promoter
    findings: []
  - id: Reactome:R-HSA-3700997
    title: TP53 binds the MDM2 gene
    findings: []
  - id: Reactome:R-HSA-3786258
    title: TP53 binds the CDKN1A promoter
    findings: []
  - id: Reactome:R-HSA-4331331
    title: TP53 binds the PMAIP1 (NOXA) promoter
    findings: []
  - id: Reactome:R-HSA-4331340
    title: TP53 binds the BBC3 (PUMA) promoter
    findings: []
  - id: Reactome:R-HSA-4395236
    title: TP53 binds promoters of MIR34 genes
    findings: []
  - id: Reactome:R-HSA-4647593
    title: EP400 binds CDKN1A promoter
    findings: []
  - id: Reactome:R-HSA-5628871
    title: BRD7 promotes EP300-mediated acetylation of TP53
    findings: []
  - id: Reactome:R-HSA-5628899
    title: TP53 binds the TIGAR gene
    findings: []
  - id: Reactome:R-HSA-5629187
    title: TP53 binds regulatory elements of SESN1,2,3 genes
    findings: []
  - id: Reactome:R-HSA-5632759
    title: TP53 binds the SCO2 gene
    findings: []
  - id: Reactome:R-HSA-5632887
    title: TP53 binds the RRM2B gene
    findings: []
  - id: Reactome:R-HSA-5632914
    title: TP53 binds the GLS2 promoter
    findings: []
  - id: Reactome:R-HSA-5632939
    title: TP53 binds the PTEN promoter
    findings: []
  - id: Reactome:R-HSA-5633295
    title: TP53 binds the NDRG1 gene promoter
    findings: []
  - id: Reactome:R-HSA-5633414
    title: TP53 binds TNFRSF10A,TNFRSF10B,TNFRSF10C,TNFRSF10D genes
    findings: []
  - id: Reactome:R-HSA-5633460
    title: MDM2 binds TP53
    findings: []
  - id: Reactome:R-HSA-5689950
    title: USP7 deubiquitinates TP53,MDM2,MDM4,FOXO4, PTEN
    findings: []
  - id: Reactome:R-HSA-5689973
    title: USP10,USP24,USP42 deubiquitinate TP53
    findings: []
  - id: Reactome:R-HSA-5690843
    title: OTUB1, (OTUB2) binds RNF128, TRAF3, TRAF6, RHOA
    findings: []
  - id: Reactome:R-HSA-5693609
    title: ATM phosphorylates TP53 at S15
    findings: []
  - id: Reactome:R-HSA-6789960
    title: Expression of NDN,TP53
    findings: []
  - id: Reactome:R-HSA-6791285
    title: TP53 binds the BID gene promoter
    findings: []
  - id: Reactome:R-HSA-6791302
    title: TP53 binds the AIFM2 gene promoter
    findings: []
  - id: Reactome:R-HSA-6791327
    title: TP53 binds the BCL2L14 gene
    findings: []
  - id: Reactome:R-HSA-6791349
    title: TP53 binds the APAF1 gene promoter
    findings: []
  - id: Reactome:R-HSA-6791387
    title: TP53 binds the ARID3A gene
    findings: []
  - id: Reactome:R-HSA-6791409
    title: TP53 binds the CCNG1 gene
    findings: []
  - id: Reactome:R-HSA-6793685
    title: Ubiquitinated TP53 translocates to the cytosol
    findings: []
  - id: Reactome:R-HSA-6796649
    title: TP53 binds the CCNK gene
    findings: []
  - id: Reactome:R-HSA-6797766
    title: TP53 binds the BIRC5 gene
    findings: []
  - id: Reactome:R-HSA-6797993
    title: TP53 and CREBBP bind BNIP3L gene
    findings: []
  - id: Reactome:R-HSA-6798020
    title: TP53 binds the BTG2 gene
    findings: []
  - id: Reactome:R-HSA-6798082
    title: TP53 family members bind the CASP1 gene
    findings: []
  - id: Reactome:R-HSA-6798129
    title: TP53 binds the CASP6 gene
    findings: []
  - id: Reactome:R-HSA-6798138
    title: TP53 binds the CASP10 gene
    findings: []
  - id: Reactome:R-HSA-6798282
    title: TP53 and E2F4 bind the CDC25C gene
    findings: []
  - id: Reactome:R-HSA-6798304
    title: TP53 binds the E2F7 gene
    findings: []
  - id: Reactome:R-HSA-6798374
    title: DYRK2 phosphorylates TP53
    findings: []
  - id: Reactome:R-HSA-6798615
    title: TP53 binds the TP53AIP1 gene
    findings: []
  - id: Reactome:R-HSA-6799246
    title: CHEK1 phosphorylates TP53
    findings: []
  - id: Reactome:R-HSA-6799332
    title: ATR phosphorylates TP53
    findings: []
  - id: Reactome:R-HSA-6799409
    title: HIPK2 phosphorylates TP53
    findings: []
  - id: Reactome:R-HSA-6799418
    title: TP53 binds the TP53INP1 gene
    findings: []
  - id: Reactome:R-HSA-6799431
    title: TP53 binds HIPK1
    findings: []
  - id: Reactome:R-HSA-6799462
    title: 'TODO: Fetch title'
    findings: []
  - id: Reactome:R-HSA-6799761
    title: 'TODO: Fetch title'
    findings: []
  - id: Reactome:R-HSA-6799777
    title: 'TODO: Fetch title'
    findings: []
  - id: Reactome:R-HSA-6799815
    title: 'TODO: Fetch title'
    findings: []
  - id: Reactome:R-HSA-6800042
    title: 'TODO: Fetch title'
    findings: []
  - id: Reactome:R-HSA-6800253
    title: 'TODO: Fetch title'
    findings: []
  - id: Reactome:R-HSA-6800279
    title: 'TODO: Fetch title'
    findings: []
  - id: Reactome:R-HSA-6800836
    title: 'TODO: Fetch title'
    findings: []
  - id: Reactome:R-HSA-6801087
    title: 'TODO: Fetch title'
    findings: []
  - id: Reactome:R-HSA-6801166
    title: 'TODO: Fetch title'
    findings: []
  - id: Reactome:R-HSA-6801209
    title: 'TODO: Fetch title'
    findings: []
  - id: Reactome:R-HSA-6801355
    title: 'TODO: Fetch title'
    findings: []
  - id: Reactome:R-HSA-6801641
    title: 'TODO: Fetch title'
    findings: []
  - id: Reactome:R-HSA-6802165
    title: 'TODO: Fetch title'
    findings: []
  - id: Reactome:R-HSA-6803391
    title: 'TODO: Fetch title'
    findings: []
  - id: Reactome:R-HSA-6803425
    title: 'TODO: Fetch title'
    findings: []
  - id: Reactome:R-HSA-6803719
    title: 'TODO: Fetch title'
    findings: []
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    title: 'TODO: Fetch title'
    findings: []
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    title: 'TODO: Fetch title'
    findings: []
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    title: 'TODO: Fetch title'
    findings: []
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    title: 'TODO: Fetch title'
    findings: []
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    title: 'TODO: Fetch title'
    findings: []
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    title: 'TODO: Fetch title'
    findings: []
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    title: 'TODO: Fetch title'
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    title: 'TODO: Fetch title'
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    title: 'TODO: Fetch title'
    findings: []
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    title: 'TODO: Fetch title'
    findings: []
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    title: 'TODO: Fetch title'
    findings: []
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    title: 'TODO: Fetch title'
    findings: []
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    title: 'TODO: Fetch title'
    findings: []
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    title: 'TODO: Fetch title'
    findings: []
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    title: 'TODO: Fetch title'
    findings: []
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    title: 'TODO: Fetch title'
    findings: []
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    title: 'TODO: Fetch title'
    findings: []
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    title: 'TODO: Fetch title'
    findings: []
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    title: 'TODO: Fetch title'
    findings: []
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    title: 'TODO: Fetch title'
    findings: []
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    title: 'TODO: Fetch title'
    findings: []
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    title: 'TODO: Fetch title'
    findings: []
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    title: 'TODO: Fetch title'
    findings: []
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    title: 'TODO: Fetch title'
    findings: []
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    title: 'TODO: Fetch title'
    findings: []
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    title: 'TODO: Fetch title'
    findings: []
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    title: 'TODO: Fetch title'
    findings: []
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    title: 'TODO: Fetch title'
    findings: []
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    title: 'TODO: Fetch title'
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    title: 'TODO: Fetch title'
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    title: 'TODO: Fetch title'
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  - id: file:human/TP53/TP53-deep-research-falcon.md
    title: Deep research report on TP53
    findings: []
core_functions:
  - molecular_function:
      id: GO:0000976
      label: transcription cis-regulatory region binding
    directly_involved_in:
      - id: GO:0030330
        label: DNA damage response, signal transduction by p53 class mediator
      - id: GO:0045930
        label: negative regulation of mitotic cell cycle
      - id: GO:0072332
        label: intrinsic apoptotic signaling pathway by p53 class mediator
    locations:
      - id: GO:0005634
        label: nucleus
    description: p53 functions as a sequence-specific DNA-binding transcription 
      factor that binds p53 response elements to regulate transcription of 
      target genes involved in cell cycle arrest, apoptosis, DNA repair, and 
      metabolism
    supported_by:
      - reference_id: PMID:15710329
        supporting_text: Human MUC1 oncoprotein regulates p53-responsive gene 
          transcription
      - reference_id: PMID:17996705
        supporting_text: An acetylation switch in p53 mediates holo-TFIID 
          recruitment
      - reference_id: file:human/TP53/TP53-uniprot.txt
        supporting_text: Multifunctional transcription factor
  - molecular_function:
      id: GO:0000976
      label: transcription cis-regulatory region binding
    directly_involved_in:
      - id: GO:0045944
        label: positive regulation of transcription by RNA polymerase II
      - id: GO:0000122
        label: negative regulation of transcription by RNA polymerase II
      - id: GO:2000772
        label: regulation of cellular senescence
    locations:
      - id: GO:0016604
        label: nuclear body
    description: p53 localizes to nuclear bodies and regulates both activation 
      and repression of transcription, including senescence programs
    supported_by:
      - reference_id: PMID:10360174
        supporting_text: ARF exon 2 disrupt its nucleolar localization and 
          impair its ability to block nuclear export of MDM2 and p53
      - reference_id: file:human/TP53/TP53-uniprot.txt
        supporting_text: induces cell cycle arrest, DNA repair or apoptosis upon
          binding to its target DNA sequence ... Negatively regulates cell 
          division
suggested_questions:
  - question: How does p53 integrate diverse stress signals to determine cell 
      fate decisions between survival, senescence, and apoptosis?
  - question: What determines the selectivity of p53 for different target gene 
      promoters and how is this modulated by post-translational modifications?
  - question: How do p53 isoforms and mutant forms interact to modulate tumor 
      suppressor function in heterozygous cancer cells?
suggested_experiments:
  - description: Single-cell time-lapse imaging to track p53 dynamics and 
      correlate oscillation patterns with specific cell fate outcomes
  - description: ChIP-seq coupled with PRO-seq to map p53 binding and 
      transcriptional outcomes under different stress conditions
  - description: Proximity labeling proteomics to identify context-specific p53 
      interactors that determine target gene selectivity
tags:
  - ferroptosis
  - lbnl-favorites
status: COMPLETE

πŸ”— Related Resources

πŸ“Š View Pathway Visualization Interactive pathway diagram with detailed annotations